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Chemistry: Molecular Biology And Microbiology > Process Of Mutation, Cell Fusion, Or Genetic Modification > Introduction Of A Polynucleotide Molecule Into Or Rearrangement Of Nucleic Acid Within An Animal Cell > The Polynucleotide Is Encapsidated Within A Virus Or Viral Coat The Polynucleotide Is Encapsidated Within A Virus Or Viral CoatThe Polynucleotide Is Encapsidated Within A Virus Or Viral Coat patent applications listed are from June 2005 to current and include Date, Patent Application Number, Patent Title, Patent Abstract summary and are linked to the corresponding patent application page.03/15/07 - 20070059831 - Nucleic acids, polypeptides, compositions, and methods for modulating apoptosis The present invention relates to isolated and/or purified rat apoptosis-specific eucaryotic initiation Factor-5A (eIF-5A) and deoxyhypusine synthase (DHS) nucleic acids and polypeptides. The present invention also relates to methods of modulating apoptosis using apoptosis-specific eIF-5A and DHS, and antisense oligonucleotides and expression vectors of apoptosis-specific eIF-5A and DHS useful in ... 03/01/07 - 20070048865 - Method for acceleration of stem cell differentiation The present invention relates to methods for accelerating the differentiation of embryonic stem cells by introducing nucleic acid encoding the Tal1/Scl transcription factor into the cells, and culturing the transduced cells in a differentiation medium. ... 02/22/07 - 20070042494 - Heterologous retroviral packaging system A chimeric retroviral vector comprising sequences from at least two retroviruses, wherein at least one of the sequences encodes a cis element, and wherein the chimeric retroviral vector is capable of being packaged in a viral particle; and methods of making and using the same. ... 02/15/07 - 20070037284 - Vectors for expressing exogenous gene or exogenous nucleic acid sequences Provided are novel vectors and viral vectors capable of expressing exogenous gene or exogenous nucleic acid sequences in a target cell of interest, such as T cells, bone marrow cells, epithelial cells, liver cells and the like. The nucleic acid components of the vectors may include one or more native ... 02/01/07 - 20070026521 - Polynucleotides for use in recombinant adeno-associated virus virion production Accessory functions capable of supporting efficient recombinant AAV (rAAV) virion production in a suitable host cell are provided. The accessory functions are in the form of one or more vectors that are capable of being transferred between cells. Methods of producing rAAV virions are also provided. The methods can be ... 01/25/07 - 20070020760 - Medium supplement and animal cell culture medium An object of the present invention is to provide a medium supplement for animal cell culture and an animal cell culture medium. The present invention relates to a medium supplement for animal cell culture comprising sericin or a sericin derivative and an animal cell culture medium comprising at least said ... 01/11/07 - 20070010017 - Lentiviral packaging constructs The present invention provides novel lentiviral packaging constructs that are useful for the establishment of stable packaging cell lines and producer cell lines. In particular, the present invention provides novel packaging cell lines that are capable of constitutively expressing high levels of lentiviral proteins. ... 01/11/07 - 20070010016 - Gene transfer with adenoviruses having modified fiber proteins Methods and compositions for transducing tumor cells using adenoviral vectors which comprise: a chimeric or modified adenovirus fiber protein and the coding sequence for a therapeutic agent, are provided. The chimeric or modified adenovirus fiber protein has at least a portion of an adenovirus fiber shaft of a first serotype ... 01/11/07 - 20070010015 - Complete empty viral particles of infectious bursal disease virus (ibdv), production method thereof and applications of same Whole empty viral particles of infectious bursal disease virus (IBDV), which contain all of the antigenically-relevant proteinaceous constituents present in determinant IBDV virions. The whole empty virus particles are readily produced in suitable expression systems to provide capsids that can be used in the production of vaccines against avian disease, ... 01/04/07 - 20070004042 - Compositions and methods for helper-free production of recombinant adeno-associated viruses A method for producing recombinant adeno-associated virus in the absence of contaminating helper virus or wild-type virus involves culturing a mammalian host cell containing an rAd/AAV hybrid virus, an AAV rep sequence and an AAV cap sequence under the control of regulatory sequences directing expression thereof. The rAd/AAV hybrid virus ... 12/14/06 - 20060281180 - Vectors Provided is a lentiviral vector capable of delivering a nucleotide of interest (NOI) to a desired target site and wherein the NOI encodes the Factor VIII and the Factor VIII is expressed following delivery of the NOI to the desired target site. ... 12/07/06 - 20060275903 - Rna interference mediated inhibition of gene expression using chemically modified short interfering nucleic acid (sina) The present invention concerns methods and reagents useful in modulating gene expression in a variety of applications, including use in therapeutic, diagnostic, target validation, and genomic discovery applications. Specifically, the invention relates to synthetic chemically modified small nucleic acid molecules, such as short interfering nucleic acid (siNA), short interfering RNA ... 11/30/06 - 20060270042 - High-titer retroviral packaging cells The present invention relates to non-replicative recombinant retrovirus packaging cells able to grow in suspension in a serum-free medium. In particular, the present invention relates to a human embryonic 293SF-based cell line stably expressing gag and pol gene products from the murine Moloney leukemia virus (MLV) and the feline RD114 ... 11/30/06 - 20060270041 - Cell lines for production of replication-defective adenovirus The present invention provides cell lines for the production of E1-deleted adenovirus (rAd) vectors that complement E1A and E1B functions. The present invention also provides cell lines for the production of E1- and E2-deleted adenovirus vectors that complement E1A, E1B and E2B polymerase functions. The invention provides particular cell lines ... 11/23/06 - 20060263883 - Recombinant herpes viruses for preparing recombinant adeno-associated viruses This document describes a recombinant herpesvirus which comprises the AAV rep and cap genes and also a process for producing high-titer, highly infectious adeno-associated virus vector preparations. ... 11/16/06 - 20060258007 - Regulated vectors for selection of cells exhibiting desired phenotypes The present invention relates to expression vectors containing nucleic acid sequences encoding one or more proteins of interest linked to one or more selection markers that can be used to select cells null for such vector and to such null cells. ... 11/16/06 - 20060258006 - Retroviral vector Provided herein is a retroviral vector comprising, and capable of expressing, a nucleotide of interest (NOI), wherein the NOI encodes an RNA or protein which is harmful to a cell. ... 11/16/06 - 20060258005 - Novel virus vector There is provided a virus vector characterized in that a water-soluble polymer is directly or indirectly linked to a surface of a virus particle and a heterogeneous peptide having an affinity for integrin present on a surface of a target cell is linked to the water-soluble polymer. ... 11/09/06 - 20060252154 - Assay to identify estrogen receptor dependent ligands that regulate the hepatic lipase promoter The present invention relates to an assay system and method for testing compounds for their ability to regulate the hepatic lipase (HL) promoter. In particular, the invention relates to the identification of estrogen receptor ligands having this activity. Compounds that inhibit HL promoter activity are useful as leads, or on ... 11/09/06 - 20060252153 - Retroviral vectors for delivery of interfering rna Provided herein are retroviral vectors for delivering interfering RNA into cells. ... 11/02/06 - 20060246587 - Methods for transfecting t cells A method for transfecting T cells with a nucleic acid molecule comprising a gene such that the gene is expressed in the T cells is described. The T cells are stimulated and proliferating prior to introduction of the nucleic acid molecule. ... 10/05/06 - 20060223184 - Supports useful in incorporating biomolecules into cells and methods of using thereof Described herein are supports useful in incorporating biomolecules into cells and methods of making and using thereof. ... 09/28/06 - 20060216824 - Paramyxovirus vector encoding ribozyme and utilization thereof The present invention provides paramyxoviral vectors encoding active ribozymes. The vectors of the present invention are suitable as vectors for gene therapy to express desired ribozymes in a broad range of tissues in vivo or ex vivo. The vectors of the present invention can be used in gene therapy for ... 09/21/06 - 20060211115 - Methods of generating chimeric adenoviruses and uses for such chimeric aden oviruses A method for providing an adenovirus from a serotype which does not grow efficiently in a desired cell line with the ability to grow in that cell line is described. The method involves replacing the left and right termini of the adenovirus with the corresponding termini from an adenovirus which ... 09/14/06 - 20060205080 - Formulations for therapeutic viruses having enhanced storage stability Therapeutic viral formulations having enhanced storage stability are described. The formulations comprise a viral vector in addition to one or more of an aqueous cosolvent, a reversible viral-encoded protease inhibitor and a mild reducing agent or other agent that prevents specific degradation of viral components. ... 09/14/06 - 20060205079 - Amplifiable adeno-associated virus (aav) packaging cassettes for the production of recombinant aav vectors High-efficiency AAV packaging constructs and methods for their use are provided in the present invention. These high-efficiency packaging constructs comprise an activating element (such as the P1 sequence located within the AAV S1 integration site of human chromosome 19) amplifiably linked to one or more AAV packaging genes. The constructs ... 09/07/06 - 20060199266 - Caev-based vector systems This invention relates to caprine arthritis encephalitis virus-based vectors and vector systems that are useful in the delivery of nucleic acids to both non-dividing and dividing cells. Methods for delivering nucleic acids to both non-dividing and dividing cells using the vector systems are also disclosed. ... 08/24/06 - 20060188991 - Flexible vaccine assembly and vaccine delivery platform Herein-described are various methods for making a vaccine that are made of re-assembled virus like particles (VLP). First, the VLPs are disassembled into encapsidation intermediate populations. Each encapsidation intermediate population undergoes, for instance, chemical conjugation of unique peptide or nucleic moieties to form separate populations. Thereafter, a predetermined amount of ... 08/24/06 - 20060188990 - Novel prostate tumor-specific promoter This invention provides novel transcriptional regulatory elements from the human TRPM4 (Transient Receptor Potential-Melastatin 4) gene. These promoter and enhancer elements preferentially activate transcription in prostate tumor cells and tissues as compared to other tissues. Methods and compositions are provided to employ TRPM4 promoter elements for prostate tumor-specific expression of ... 08/17/06 - 20060183232 - Packaging cells for recombinant adenovirus In the absence of substantial sequence overlap between a recombinant adenoviral vector and the genome of a packaging cell, helper-dependent E1-containing particles (HDEP) can be formed at low frequency. The invention provides means and methods reducing or preventing the generation of HDEP. To this purpose, novel packaging cells and methods ... 08/17/06 - 20060183231 - Multiple-compartment eukaryotic expression systems Method and constructs for expressing heterologous sequences of interest in eukaryotic cells using multiple-compartment expression systems. These systems, which may be comprised of a single construct or multiple constructs, utilize at least two different promoters which are each active within a different subcellular compartment of the same eukaryotic cell. The ... 08/17/06 - 20060183230 - Vectors, cell lines and their use in obtaining extended episomal maintenance replication of hybrid plasmids and expression of gene products This disclosure shows that the BPV-1 E2 protein-dependent minichromosome maintenance element (MME) comprised of E2 multimeric binding sites can provide the stable maintenance replication function to the mouse polyomavirus (PyV) core origin plasmids in the presence of BPV-1 E2 protein and PyV large T-antigen (LT). MME dependent plasmids are lost ... 08/17/06 - 20060183229 - Regulatory constructs comprising intron 3 of prostate specific membrane antigen gene The invention provides a recombinant vector comprising an ovine adenovirus genome and a sequence encoding a heterologous polypeptide, wherein the sequence encoding the heterologous polypeptide is inserted between E4 and E3 transcription units of the ovine adenovirus genome. ... 08/17/06 - 20060183228 - viral vectors with surface or envelope components Provided are novel vectors and viral vectors capable of expressing exogenous gene or exogenous nucleic acid sequences in a target cell of interest, such as T cells, bone marrow cells, epithelial cells, liver cells and the like. The nucleic acid components of the vectors may include one or more native ... 08/17/06 - 20060183227 - Method for gene transfer into the organelles of cells: direct gene transfer to mitochondria Methods of gene transfer into cellular organelles are disclosed which provide the necessary conditions for genetic materials such as virus or recombinant DNA to be delivered directly into mitochondria from extracellular space in vitro or in vivo. Once inside the mitochondria, the genetic materials can then be translated according to ... 08/17/06 - 20060183226 - Methods for retrotransposing long interspersed elements (lines) The present invention provides methods for retrotransposing LINEs. The present invention relates to methods for transcribing RNAs comprising LINE 3′UTR fragments in cells, and retrotransposing these RNAs by using viral vectors to provide their LINE ORF proteins in trans. This invention also relates to methods for altering LINE retrotransposition target ... 08/10/06 - 20060177934 - Lentiviral ltr-deleted vector A vector capable of transducing non-dividing and/or slowly dividing cells is provided, wherein the vector is a lentiviral LTR-deleted vector. Also provided is a method for producing a protein of interest in a non-dividing or slowly dividing cell by transducing the cell with a lentiviral LTR-deleted vector and expressing the ... 08/10/06 - 20060177933 - Expression of apoa-1 and variants thereof using spliceosome mediated rna trans-splicing The present invention provides methods and compositions for generating novel nucleic acid molecules through targeted spliceosome mediated RNA trans-splicing that result in expression of an apoA-1 variant, the preferred embodiment referred to herein as the apoA-1 Milano variant. The compositions of the invention include pre-trans-splicing molecules (PTMs) designed to interact ... 08/10/06 - 20060177932 - Expansion agents for stem cells The present invention relates to a method and substance for maintaining the pluripotency and self-replication ability of a stem cell, such as a hematopoietic stem cell, while keeping it undifferentiated. Specifically, the present invention provides a composition for maintaining the expansion or pluripotency of a stem cell, comprising active STAT5, ... 08/03/06 - 20060172421 - Novel cosmid vector A novel cosmid vector and the like effectively used in generating a recombinant adenoviral vector are provided. More specifically, there are provided a cosmid vector characterized by: (1) containing an adenoviral genome having adenoviral inverted terminal repeat sequences each having a complete nucleotide sequence, (2) having a deletion in an ... 07/27/06 - 20060166364 - Use of flexible bag containers for viral production The present invention relates generally to the fields of cell banking and viral production. More particularly, it concerns a method of virus production from host cells using flexible containers. ... 07/27/06 - 20060166363 - Modified baculovirus expression system for production of pseudotyped raav vector The invention provides modifications to a baculovirus-based recombinant adeno associated virus (AAV) system including enhancement of the helper virus stability and construction of novel baculovirus vectors for rAAV pseudotyping. The modified system extends the flexibility of rAAV vector production and promotes the utility of AAV as, a clinically applicable gene ... 07/20/06 - 20060160220 - Retroviral vectors with introns The present invention relates to improved retroviral vectors. In particular, the present invention relates to retroviral vectors that retain introns in genes of interest during vector production. The present invention further provides host cells and animals comprising gene delivered by the vectors. The present invention additionally provides methods of using ... 07/13/06 - 20060154370 - Efficient gene suppression using a transfer rna promoter in herpes virus vectors to deliver small interference rnas The invention provides herpes virus nucleic acid vectors for expressing shRNAs in mammalian cells and thereby silencing target genes. The vectors include (a) a herpes virus packaging signal sequence; (b) a herpes virus origin of replication; (c) a segment expressing a light-emitting marker; and (d) a transfer RNA promoter upstream ... 07/13/06 - 20060154369 - Promoter sequences from wssv immediate early genes and their uses in recombinant dna techniques Disclosed herein are isolated promoter-regulatory regions from a newly identified WSSV immediate early (IE) gene, ie1 (immediate early gene #1), which exhibit promoter activity to drive the transcription of a target gene in non-native host cells. The isolated promoter-regulatory regions can be used in the construction of a variety of ... 07/06/06 - 20060148087 - Development of a preventive vaccine for filovirus infection in primates The present invention relates generally to viral vaccines and, more particularly, to filovirus vaccines and methods of eliciting an immune response against a filovirus or disease caused by infection with filovirus. ... 06/15/06 - 20060128020 - Methods of unidirectional, site-specific integration into a genome, compositions and kits for practicing the same The subject invention provides a unidirectional site-specific integration system for integrating a nucleic acid into the genome of a target cell. The provided system includes a site-specific integrating expression cassette (INTEC) vector, consisting of (a) a polynucleotide of interest operably linked to a promoter, (b) a single recombination site, and ... 06/15/06 - 20060128019 - Process for producing virus vector containing membrane protein having sialic acid-binding in envelope with the use of gram-positive bacterium origin nueraminidase The present invention provides methods for producing a viral vector comprising a membrane protein that binds to sialic acid as a component of the envelope, using neuraminidase (NA) derived from Gram-positive bacteria. The methods comprise the steps of culturing cells producing a viral vector in the presence of an NA ... 06/08/06 - 20060121611 - Fed-batch process for production of erythropoietin in human embryonic retina cells that express adenovirus e1a The invention provides processes for recombinant production of erythropoietin (EPO) in a human embryonic retina cell that expresses at least an adenoviral E1A protein, wherein said EPO is produced at high concentrations and wherein said EPO as produced has a high average sialic acid content per EPO molecule. ... 05/11/06 - 20060099710 - Vector for improved in vivo production of proteins A vector designed to include two tags wherein the first tag improves protein expression, folding and solubility, and the second tag promotes affinity purification, and two distinct recognition sequences for highly specific proteases. Uses of the vector include in vivo protein production. ... 05/11/06 - 20060099709 - Targeted tumor therapy by use of recombinant adenovirus vectors that selectively replicate in hypoxic regions of tumors The presently claimed subject matter provides conditionally replication competent adenoviral vectors that confer selective cytotoxicity on cells expressing HIF-1 by infecting cells that allow HIF-1 inducible promoters present within the vectors to function. Also provided are compositions and host cells based upon the vectors, as well as methods of propagating ... 05/04/06 - 20060094116 - High-efficiency wild-type-free aav helper functions The present invention provides methods and compositions for producing high titer, wild-type-free preparations of recombinant AAV (“rAAV”) virions. The compositions of the present invention include novel nucleic acids encoding AAV helper functions and AAV helper function vectors. The present invention also includes host cells transfected by the claimed nucleic acids, ... 04/27/06 - 20060088937 - Flavivirus expression and delivery system The present invention provides a gene expression system comprising: a) a self-replicating expression vector of flavivirus origin which includes the flavivirus 5′ untranslated region (UTR), at least a portion of the 5′ coding region for flavivirus core protein, the nucleotide sequence coding for the flavivirus non-structural proteins, and the complete ... 04/27/06 - 20060088936 - Raav expression systems for genetic modification of specific capsid proteins Disclosed are improved recombinant adeno-associated viral (rAAV) vectors having mutations in one or more capsid proteins. Exemplary vectors are provided that have altered affinity for heparin or heparin sulfate, as well as vectors, expression systems, and rAAV virions that lack functional VP2 protein expression, but are nevertheless, fully virulent. Also ... 04/06/06 - 20060073594 - Chimeric vectors The present invention relates to chimeric vectors. More specifically, the invention relates to recombinant poxvirus vectors and viruses that are capable of expressing an alphaviral RNA replicon expressing a heterologous sequence of interest. ... 03/23/06 - 20060063261 - Production of vaccines Means and methods for producing mammalian viruses, the method comprising infecting a culture of immortalized human cells with a virus, incubating the culture infected with virus to propagate the virus under conditions that permit growth of the virus, and to form a virus-containing medium, and removing the virus-containing medium. The ... 03/23/06 - 20060063260 - Method for producing a support carrying immobilized viruses, and the use of such a support A method for producing a support possessing a functionalized surface which is suitable for immobilizing viruses and carrying viruses which are immobilized on the functionalized surface and which are preferably infectious, as well as the use of such supports, are described. In this connection, a support material is initially provided ... 03/23/06 - 20060063259 - Production of adenovirus vectors with reduced levels of replication competent adenovirus contamination Methods, cells and recombinant adenoviral vectors are disclosed that permit the production of recombinant adenoviral vector stocks with reduced levels of contamination by replication competent adenoviruses (RCA). In certain embodiments are disclosed early region 1 (E1) deficient recombinant adenoviral vectors and complementing E1 positive host cells whose sequences are designed ... 03/23/06 - 20060063258 - Retinoic acid receptor beta-2, its agonists, and gene therapy vectors for the treatment of neurological disorders The present invention relates to the use of RARβ2 and/or an agonist thereof in the preparation of a medicament to cause neurite development. ... 03/16/06 - 20060057725 - Therapeutic retroviral vectors for gene therapy Retroviral gene therapy vectors that are optimized for erythroid specific expression and treatment of hemoglobinopathic conditions are disclosed. ... 03/02/06 - 20060046294 - Site-specific recombination systems for use in eukaryotic cells Prokaryotic recombination systems have been adapted to function in eukaryotes in order to achieve one or more of the following: DNA site specific excision, translocation, integration and inversion. These recombination systems are identified as seven members of the small serine resolvase subfamily: CinH, ParA, Tn1721, Tn5053, Tn21, Tn402, and Tn501 ... 02/16/06 - 20060035378 - Modified viral vector particles This invention relates to viral vector particles, including capsid proteins with an attachment site for the specific chemical modification of the vector particles. Furthermore, the invention relates to procedures for the production of these viral vector particles. Furthermore, the invention relates to the use of these viral vector particles as ... 02/09/06 - 20060030046 - Method for gene transfer into target cells with retrovirus A polypeptide represented by SEQ. ID No. 13, a polypeptide represented by SEQ. ID No. 30 or functional equivalents thereof and a polypeptide represented by SEQ. ID No. 17. ... 01/26/06 - 20060019396 - Retroviral vectors with enhanced efficiency of transgene expression and safety The present application discloses a MoMSV/MoMLV hybrid-based retroviral vector, wherein gag and pol genes are completely removed. ... 01/26/06 - 20060019395 - Lentiviral vectors and uses thereof The invention relates to compositions containing polynucleotide vectors capable of expressing a nucleic acid encoding a fusion polypeptide on the surface of a viral particle and/or a eukaryotic cell. ... 01/26/06 - 20060019394 - Bovine immunodeficiency virus (biv) based vectors This invention pertains to BIV constructs encompassing BIV combination vectors, BIV vectors and BIV packaging vectors and particularly the invention pertains to a three vector system comprising: a) a BIV vector construct including a DNA segment from a BIV genome, a packaging sequence to package RNA into virions; a promoter ... 01/26/06 - 20060019393 - Minimal lentiviral vector system A lentiviral vector system is described. The system comprises a lentiviral transfer vector and a packaging construct. The transfer vector comprises (a) a 5′ LTR; (b) a 3′ LTR comprising a polyadenylation signal; (c) a minimal packaging signal, (d) (i) at least one heterologous upstream enhancer (UE) sequences, and/or (ii) ... 01/19/06 - 20060014288 - Nerve regeneration The present application describes a method of regenerating nerve, which steps include generating a recombinant viral or plasmid vector comprising a DNA sequence encoding a member of a transforming growth factor superfamily of proteins operatively linked to a promoter; transfecting in vitro a population of cultured cells with the recombinant ... 01/05/06 - 20060003453 - High-efficiency aav helper functions The present invention provides methods and compositions for producing high titer preparations of recombinant AAV (“rAAV”) virions. The compositions of the present invention include AAV helper function systems and host cells. The present invention also includes methods of using AAV helper function vectors that effect the production of only small ... 01/05/06 - 20060003452 - Vector packaging cell line The invention relates to a method of increasing vector transduction in target cells. The invention provides for the recombinant engineering of a packaging cell line to be capable of expressing one or more membrane proteins which facilitate binding to, and activation of, a target cell. The invention also provides for ... 01/05/06 - 20060003451 - Cell lines and constructs useful in production of e1-deleted adenoviruses in absence of replication competent adenovirus Novel cell lines useful for trans-complementing E1-deleted adenoviral vectors are described. The cell lines are capable of providing high yields of E1-deleted adenoviral vectors in the absence of replication-competent adenovirus over multiple passages. ... 12/29/05 - 20050287672 - Avipox recombinants expressing foot and mouth disease virus genes The present invention relates to modified poxviral vectors and to methods of making and using the same. In particular, the invention relates to recombinant avipox that expresses gene products of foot and mouth disease virus (FMDV), and to compositions or vaccines that elicit immune responses directed to FMDV gene products ... 12/29/05 - 20050287671 - Soluble steroidal peptides for nucleic acid delivery Amphiphilic lipopeptide compositions for gene delivery are disclosed. An illustrative amphiphilic lipopeptide composition includes a human protamine 2 peptide conjugated to a hydrophobic moiety. Illustrative hydrophobic moieties include sterols, bile acids, and fatty acids. The amphiphilic lipopeptide composition is mixed with a nucleic acid such that the nucleic acid binds ... 12/22/05 - 20050282282 - Methods of use of viral vectors to deliver antigen to dendritic cells This invention relates to methods and compositions useful for delivering antigens to dendritic cells which are then useful for inducing T antigen specific cytotoxic T lymphocytes. This invention also provides assays for evaluating the activity of cytotoxic T lymphocytes. According to the invention, antigens are provided to dendritic cells using ... 12/22/05 - 20050282281 - Recombinant baculoviral vector containing protein transduction domain gene A recombinant baculoviral vector, which comprises a vesicular stomatitis virus glycoprotein (VSVG) gene and a protein transduction domain (PTD) gene derived from HIV-1 (human immunodeficiency virus-1) Tat gene, is useful in a gene therapy owing to its excellent efficiencies in the delivery of a target gene to the host cell ... 12/22/05 - 20050282280 - Oncolytic adenoviral vectors encoding gm-csf Selectively replicating oncolytic adenoviral vectors comprising an adenoviral packaging signal a termination signal sequence, an E2F responsive promoter operably linked to an adenoviral coding region, a heterologous coding sequence encoding GM-CSF and a right ITR are provided. The oncolytic adenoviral vectors are useful for expressing GM-CSF in transduced cells and ... 12/22/05 - 20050282279 - Expression vector encoding coronavirus-like particle The present invention relates to a expression vector for cloning the Class I viral fusion protein gene and as DNA vaccine candidate against virus infection diseases. ... 12/15/05 - 20050277194 - Complementing cell lines A packaging cell line that complements recombinant adenoviruses based on serotypes from subgroup B, preferably adenovirus type 35. The cell line is preferably derived from primary, diploid human cells that are transformed by adenovirus E1 sequences either operatively linked on one DNA molecule or located on two separate DNA molecules, ... 12/15/05 - 20050277193 - Vectors and methods for gene transfer The present invention provides a recombinant adenovirus comprising coat proteins that lack native binding. In particular, the present invention provides a recombinant adenovirus comprising a penton base protein and a fiber protein, wherein the penton base protein and the fiber protein lack native binding. The present invention further provides a ... 12/15/05 - 20050277192 - Hepatitis c virus nonstructural protein 4a (ns4a) is an enhancer element Aspects of the present invention concern the discovery of an enhancer that regulates the expression of an associated gene. More particularly, it was found that the nonstructural protein 4A (NS4A) from the hepatitis C virus (HCV) modulates the expression and immunogenicity of an associated nucleic acid. ... 12/08/05 - 20050272155 - Methods and compositions for enhancing cell adhesion properties The present invention provides a modified cell having adhesion properties that are increased as compared to the adhesion properties of an unmodified cell, comprising a) a recombinant nucleic acid encoding an integrin β3 subunit; b) a recombinant nucleic acid encoding an integrin αv subunit; c) a recombinant nucleic acid encoding ... 12/01/05 - 20050266567 - Methods for generating high titer helper-free preparations of released recombinant aav vectors This invention provides methods and compositions for producing high titer, substantially purified preparations of recombinant adeno-associated virus (AAV) that can be used as vectors for gene delivery. At the onset of vector production, AAV producer cells of this invention typically comprise one or more AAV packaging genes, an AAV vector ... 12/01/05 - 20050266566 - Recombinant sendai virus A method for regenerating Sendai virus particles by transfecting the Sendai virus genome to a host expressing all genes for the initial viral replication has been developed, enabling the genetic manipulation of Sendai virus and effective utilization of said virus as the vector. ... 12/01/05 - 20050266565 - Novel lentiviral vectors for site-specific gene insertion Murine leukemia virus (MLV) and lentivirus vectors have been used previously to deliver genes to hematopoietic stem cells (HSCs) in human gene therapy trials. However, these vectors integrate randomly into the host genome, leading to disruption or inactivation of vital host genes. The present invention discloses a novel lentiviral vector ... 12/01/05 - 20050266564 - Tetracycline-regulated gene expression in hsv-1 vectors The present invention is directed to HSV-1 vectors which rely on the tetracycline repressor and operator as a means for regulating expression. The vectors utilize VP-16 responsive promoters of HSV to control expression of the tetracycline repressor. The vectors are of particular interest as vehicles for recombinantly expressing genes in ... 11/17/05 - 20050255597 - Method and means for producing high titer, safe, recombinant lentivirus vectors Lentiviral vectors modified at the 5′ LTR or both the 5′ and 3′ LTR's are useful in the production of recombinant lentivirus vectors. Such vectors can be produced in the absence of a functional tat gene. Multiple transformation of the host cell with the vector carrying the transgene enhances virus ... 10/27/05 - 20050239205 - Gb virus b based replicons and replicon enhanced cells The present invention features GBV-B replicons and replicon enhanced cells. A GBV-B replicon is an RNA molecule able to autonomously replicate in a cultured cell and produce detectable levels of one or more GBV-B proteins. GBV-B replicon enhanced cells are cells having an increased ability to maintain a GBV-B replicon. ... 10/20/05 - 20050233457 - Recombining viral vectors for the tetracycline-regulated expression of genes The invention relates to recombining viral vectors which can be suppressed in a highly efficient manner by means of tetracycline or tetracycline derivatives, and the use thereof for expressing genes in eukaryotic cells, particularly within the framework of gene therapy. ... 10/20/05 - 20050233456 - In vivo production of cyclic peptides The present invention relates ti methods and compositions utilizing inteins to generated libraries of cyclic peptides in vivo. ... 10/06/05 - 20050221493 - Recombinant virus production for the manufacturing of vaccines The present invention relates to the production of recombinant viruses and/or recombinant viral proteins using cells that can grow in suspension and in serum-free conditions without the requirement of any animal- or human-derived components. In particular, the invention relates to the production of recombinant alphaviruses that are suitable for use ... 10/06/05 - 20050221492 - Means and methods for nucleic acid delivery vehicle design and nucleic acid transfer Cells capable of at least, in part, complementing adenovirus an adenovirus defective in E2A function. Such cells include a nucleic acid-encoding adenovirus E2A or a functional part, derivative, temperature-sensitive mutation and/or analogue thereof, integrated into the cell's genome. Methods for producing an adenovirus particle/vector with a functional deletion of E2A ... 10/06/05 - 20050221491 - In vivo selection method for determining inhibitory rna molecules A selection system suitable for use in vivo is provided, the system comprising: I) a plurality of first nucleotide sequences encoding a gene product capable of binding to and effecting the cleavage, directly or indirectly, of a nucleotide sequence, or a transcription product thereof; wherein a region of the first ... 10/06/05 - 20050221490 - Dna virus microrna and methods for inhibiting same The invention relates to isolated nucleic acid molecules comprising the sequence of a DNA virus microRNA. In another embodiment, the invention relates to single stranded DNA virus microRNA molecules. In yet another embodiment, the invention relates to single stranded anti-DNA virus microRNA molecules. ... 10/06/05 - 20050221489 - Recombinant negative strand virus rna expression systems and vaccines The present invention relates to recombinant RNA virus templates derived from and applicable to negative strand naturally non-segmented viruses, including the families Bornaviridae, Filoviridae, and Paramyxoviridae, and methods for generating such recombinant RNA virus templates, wherein the templates are RNA generated from two or more recombinant RNA molecules. The invention ... 09/29/05 - 20050214945 - Htlv-i tax induced killing of p53 null cancer cells This invention is based on the finding that expression of an exogenous nucleic acid encoding a polypeptide having human T-cell leukemia virus type I (HTLV-1) Tax activity in p53 null cells results in a sensitization of those cells to DNA damaging agents. Therefore, the present invention is directed to a ... 09/22/05 - 20050208661 - Baculovirus vector, method of producing thereof and method of gene transfer The present invention provides a novel baculovirus vector which can provide a desired protein not requiring infectivity to insect cells, on the viral particle surface; method of producing thereof; and method of gene transfer using the baculovirus vector. A method of producing a baculovirus vector including; a process of cotransfecting ... 09/22/05 - 20050208660 - Bovine immunodeficiency virus (biv) based vectors This invention pertains to BIV constructs encompassing BIV combination vectors, BIV vectors and BIV packaging vectors and particularly the invention pertains to a three vector system comprising: a) a BIV vector construct including a DNA segment from a BIV genome, a packaging sequence to package RNA into virions; a promoter ... 09/22/05 - 20050208659 - Methods for proliferating terminal differentiated cells and recombinant vectors therefor Terminal differentiated cells are proliferated by introducing a cyclin and a cyclin dependent kinase into the nucleus of terminal differentiated cells, and then cultivating or holding the cells. A method for proliferating terminal differentiated cells comprising adding a nucleotide sequence coding for a nuclear localization signal to at least one ... 09/15/05 - 20050202561 - Plasmids encoding therapeutic agents Plasmids encoding anti-HIV and anti-anthrax therapeutic agents are disclosed. Plasmid pWKK-500 encodes a fusion protein containing DP178 as a targeting moiety, the ricin A chain, an HIV protease cleavable linker, and a truncated ricin B chain. N-terminal extensions of the fusion protein include the maltose binding protein and a Factor ... 09/15/05 - 20050202560 - Lentiviral vectors derived from sivsmm/pbj14, method for their production and uses thereof The invention relates to retroviral vectors (known as lentiviral vectors), which are used to transfer genes into cells that are at cell cycle stage G0, to methods for their production and to the use thereof for transferring genes into mammalian cells. Said vectors are derived from SIVsmmPBj14 (simian immunodeficiency virus) ... 09/08/05 - 20050196865 - Gene expression system based on codon translation efficiency The present invention discloses a method for modulating the production of a protein from a polynucleotide in a CHO cell by replacing at least one codon of the polynucleotide with a synonymous codon that has a higher or lower translation efficiency in the CHO cell than the codon it replaces, ... 09/08/05 - 20050196864 - Induction and high-yield preparative purification of mesencephalic dopaminergic neuronal progenitor cells and dopaminergic neurons from human embryonic stem cells The present invention relates to an enriched or purified population of dopaminergic neuronal progenitor cells and an enriched or purified population of dopaminergic neurons. These enriched or purified populations are derived from a population of embryonic stem cells by inducing production of dopaminergic neuronal progenitor cells. A promoter or enhancer ... 09/01/05 - 20050191747 - Recombinant bovine immunodeficiency virus based gene transfer system The present invention provides recombinant lentiviral vectors and gene transfer systems which produce said vectors, cell lines utilized in the production of said recombinant lentiviral vectors, and Bovine Immunodeficiency Virus DNA sequences utilized in the recombinant vectors and gene transfer systems. ... 08/25/05 - 20050186677 - Novel mutated mammalian cells and animals Novel mutated mammalian cells are provided that have been characterized by identifying the sequence of the genes that have been mutated. Preferably, novel mutated cells are murine ES cells that stably incorporate retroviral gene trap constructs in the specifically identified genes. The novel mutated cells and animals are useful in ... 08/18/05 - 20050181507 - Gene delivery vectors with cell type specificity for mesenchymal stem cells Methods and associated materials for transducing mesenchymal stem cells with a desired nucleic acid. Mesenchymal stem cells are a recently discovered kind of stem cell for which suitable transfer vehicles are still desired. Typical gene delivery vehicles such as the adenoviruses or adeno associated viruses have no particular tropism for ... 08/11/05 - 20050176146 - Methods of virus production A simple yet effective method of increasing production of a thermo-stable virus, such as adenovirus and picornavirus, is presented. The method entails a temperature shift strategy whereby the culture of host cells are shifted to a suboptimal temperature for a period of time prior to virus infection or cells are ... 08/04/05 - 20050170507 - Novel lentiviral packaging cells Novel packaging cell lines which produce recombinant retrovirus, free of detectable helper-virus are disclosed. Also disclosed are methods of making the cell lines and methods of producing recombinant retroviruses from the cell lines. Retroviruses produced by the cell lines include lentiviruses, such as HIV, capable of transfering heterologous DNA to ... 07/28/05 - 20050164392 - Accessory functions for use in recombinant aav virion production Accessory functions capable of supporting efficient recombinant AAV (rAAV) virion production in a suitable host cell are provided. The accessory functions are in the form of one or more vectors that are capable of being transferred between cells. Methods of producing rAAV virions are also provided. The methods can be ... 07/14/05 - 20050153450 - Compositions and methods for producing recombinant adeno-associated virus The present invention provides compositions and methods of producing recombinant AAV (rAAV) virions in large amounts or high titers. Also provided are methods for producing stably transformed host cells capable of producing rAAV virions. ... 07/07/05 - 20050148078 - Bovine immunodeficiency virus (biv) based vectors This invention pertains to BIV constructs encompassing BIV combination vectors, BIV vectors and BIV packaging vectors and particularly the invention pertains to a three vector system comprising: a) a BIV vector construct including a DNA segment from a BIV genome, a packaging sequence to package RNA into virions; a promoter ... 07/07/05 - 20050148077 - Bovine immunodeficiency virus (biv) based vectors This invention pertains to BIV constructs encompassing BIV combination vectors, BIV vectors and BIV packaging vectors and particularly the invention pertains to a three vector system comprising: a) a BIV vector construct including a DNA segment from a BIV genome, a packaging sequence to package RNA into virions; a promoter ... 07/07/05 - 20050148076 - Packaging cell lines for generation of high titers of recombinant aav vectors AAV vectors may have utility for gene therapy but heretofore a significant obstacle has been the inability to generate sufficient quantities of such recombinant vectors in amounts that would be clinically useful for human gene therapy application. Stable AAV packaging cell lines have been elusive, mainly due to the activities ... 06/23/05 - 20050136539 - Reversible immortalization of human renal proximal tubular epithelial cells The present provides reversibly immortalized RPTECs and methods for making and utilizing these cells. Specifically, the present invention provides a method of reversibly immortalizing RPTECs by introducing a first vector containing a human telomerase catalytic subunit (hTERT) gene flanked by loxP sites and a second vector containing an SV40 T ... 06/16/05 - 20050130306 - Viral vectors whose replication and, optionally, passenger gene are controlled by a gene switch activated by heat in the presence or absence of a small molecule regulator The present invention relates to conditionally replicating viruses or pairs of viruses containing a gene switch that is activatable by transient heat or other proteotoxic stress in the presence or absence of a small molecule regulator. The gene switch controls the expression of a gene for a protein required for ... 06/09/05 - 20050124068 - Hepatocellular carcinoma specific promoter and uses thereof Liver cancer, particularly hepatocellular carcinoma specific promoter sequences and adenovirus vehicles are provided. By providing for transcriptional initiating regulation dependent upon transcription factors that are only active in specific, limited cell types, virus replication will be restricted to the target cells. The modified adenovirus may be used as a vehicle ... 06/02/05 - 20050118717 - Vp22 proteins and uses thereof Herpesviral VP22 proteins (and variants) are used to modify cell structure and cell division, by their newly found property of binding to microtubules in cells. Uses of VP22 to exploit this property include stabilisation of animal cellular microtubules in vivo and in vitro, e.g. to retard or arrest cell division ... ### FreshPatents.com Support |