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Chemistry: Molecular Biology And Microbiology > Process Of Mutation, Cell Fusion, Or Genetic Modification > Introduction Of A Polynucleotide Molecule Into Or Rearrangement Of Nucleic Acid Within An Animal Cell Introduction Of A Polynucleotide Molecule Into Or Rearrangement Of Nucleic Acid Within An Animal CellIntroduction Of A Polynucleotide Molecule Into Or Rearrangement Of Nucleic Acid Within An Animal Cell patent applications listed are from June 2005 to current and include Date, Patent Application Number, Patent Title, Patent Abstract summary and are linked to the corresponding patent application page.04/19/07 - 20070087436 - Microinjection method and device An object of the present invention is to provide a method for introducing a physiologically active substance such as a gene into a cell, which introduces a physiologically active substance such as any given gene into any given cell in a view under a microscope, while significantly reducing invasiveness to ... 03/29/07 - 20070072297 - Method for ligating nucleic acids and molecular cloning The invention provides methods of covalently joining nucleic acid molecules and methods of molecular cloning. The methods provide either sequential or simultaneous ligation of flanking or vector nucleic acid molecules to nucleic acid insert molecules by topoisomerase and DNA ligase. The methods provide for directional and non-directional covalent joining and ... 03/29/07 - 20070072296 - C/ebpalpha gene targeting constructs and uses thereof A DNA construct for the expression of a functional mammalian C/EBPα comprising the following components: (i) a first region having homology with an endogenous tumor-specific promoter gene sequence of a gene locus (ii) a DNA molecule encoding the C/EBPα; and (iii) a second region having homology with a region within ... 03/15/07 - 20070059830 - Method for creating myeloid cell lines The methods and compositions of the present invention find use in modulating myeloid cell development, particularly differentiation and proliferation. The compositions of the invention include isolated transgenic cells, transgenic tissue, transgenic animals, and transgenic mice. The methods allow generation of myeloid cell lines suitable for tissue culture. Further the invention ... 03/15/07 - 20070059829 - Cristin/r-spondin ligands active in the wnt signaling pathway and methods, compositions and kits relating thereto The present invention relates to compositions and method for the modulation of Wnt pathway signaling. The Wnt signaling pathway is instrumental in the regulation of cell proliferation, differentiation and morphogenesis. ... 03/15/07 - 20070059828 - Carrier for nucleic acid molecule delivery A carrier for nucleic acid molecule delivery formed from a saccharified copolymer comprising repeating unit (A) having a cationic group, saccharified repeating unit (B). There is further provided a carrier for nucleic acid molecule delivery formed from a saccharified copolymer comprising, in addition to the repeating units (A) and (B), ... 02/22/07 - 20070042493 - Method and process of genetic transformation using supercritical fluids Methods are provided for improving the ability of non-naturally transformable cells to take up and integrate DNA. Generally, the invention provides a method for transforming cells, comprising combining deoxyribonucleic acid (DNA) and a recipient cell culture for the uptake of the DNA, placing mixture of recipient cell culture and DNA ... 02/22/07 - 20070042492 - Amino acid sequences facilitating penetration of a substance of interest into cells and/or cell nuclei The present invention relates to an amino acid sequence being able to facilitate penetration of a substance of interest inside cells and/or cell nuclei and having the following formula (I) [(X1)p [(X)o (B)nX B X X B]m (X2)q Wherein X1 and X2 are amino acid sequences of 1 to 20 ... 01/11/07 - 20070010014 - Compositions and methods for enhanced delivery to target sites A set of compositions that includes an active agent-labeled species and a pretargeting conjugate is disclosed. The active agent-labeled species includes a first oligomeric nucleotide or mimic thereof conjugated to a first moiety coupled with a diagnostic active agent. The pretargeting conjugate includes a second oligomeric nucleotide or mimic thereof ... 01/04/07 - 20070004041 - Heirarchical assembly methods for genome engineering The present invention provides recombination based methods for assembling nucleic acids. In certain aspects the present invention provides hierarchical assembly methods for producing genome sized polynucleotide constructs. The methods may be used for assembling large polynucleotide constructs, for synthesizing synthetic genomes, or for introducing a plurality of nucleotide changes throughout ... 01/04/07 - 20070004040 - Rnai agents for maintenance of stem cells The present invention provides compositions and methods suitable for delivering RNAi agents against genetic targets in stem cells so as to direct cell growth and differentiation. ... 12/21/06 - 20060286671 - Efficient generation of expression cell lines through the use of scorable homeostatic reporter genes The present invention provides methods for site-specific recombination in a cell, as well as vectors which can be employed in such methods. The methods and vectors of the present invention can be used to obtain persistent gene expression in a cell and to modulate gene expression. One preferred method according ... 12/14/06 - 20060281179 - Inducer for differentiation of embryo stem cells into ectodermal cells method of obtaining the same and use thereof A method for obtaining a solution having activity to induce differentiation of an embryonic stem cell into an ectodermal cell or ectoderm-derived cell, which comprises culturing a stromal cell in a culture comprising a polyanionic compound and recovering the culture; a solution having activity to induce differentiation of an embryonic ... 11/30/06 - 20060270040 - Compositions and methods for treating tissue The present invention relates to the field of bacteriology. In particular, the invention relates to novel compositions and methods for altering (e.g., inhibiting) the growth and virulence of populations of pathogenic microorganisms. ... 11/23/06 - 20060263882 - Multi-cistronic vectors for gene transfer protocols The subject of the present invention is the construction of multicistronic eukaryotic plasmid expression vectors in which it is possible to express from two to four genes simultaneously and which are characterized by differently regulated bicistronic transcription units. The distinctive characteristic of these vectors is the presence of a CAP-independent ... 11/02/06 - 20060246586 - Chromosome-based platforms Artificial chromosomes, including ACes, that have been engineered to contain available sites for site-specific, recombination-directed integration of DNA of interest are provided. These artificial chromosomes provide tractable, efficient and rational engineering of the chromosome for a variety of applications. ... 11/02/06 - 20060246585 - Nucleic acid complex and method of introducing nucleic acid into cell using the same A nucleic acid is effectively introduced into a cell while suppressing the cytotoxicity by administering a nucleic acid complex comprising a high molecular weight derivative of ε-poly-L-lysine and a nucleic acid to a cell. ... 10/12/06 - 20060228800 - Novel transgenic methods using intronic rna The present invention relates to a method and composition for generating an artificial intron and its components capable of producing microRNA (miRNA) molecules and thus inducing specific gene silencing effects through intracellular RNA interference (RNAi) mechanisms, and the relative utilization thereof. The miRNA-producing intron so generated is not only useful ... 10/12/06 - 20060228799 - Use of consensus sequences for targeted homologous gene isolation and recombination in gene families The invention relates to compositions and methods for targeting sequence modifications in one or more genes of a related family of genes using enhanced homologous recombination techniques. These techniques may be used to create animal or plant models of disease as well as to identify new targets for drug or ... 10/12/06 - 20060228798 - Homologous recombination in multipotent adult progenitor cells The invention relates to methods of altering gene expression by homologous recombination in a multipotent adult progenitor cell (MAPC). In particular, methods of producing a recombinant MAPC, of correcting a genetic defect in a mammal, of providing a functional and/or therapeutic protein to a mammal, and of transforming a MAPC ... 09/28/06 - 20060216823 - Method for obtaining an enriched population of sirna-expressing cells Problems with transience of siRNA-mediated knock-down and transfection efficiency have limited the scope of RNAi-based experiments. The invention provides a tool for employing RNAi more efficiently and effectively by integrating RNAi expression with methods of cell enrichment. ... 09/21/06 - 20060211114 - Modulation of hedgehog-mediated signaling pathway The present invention provides transcription factors associated with the hedgehog signaling pathway that are regulated by dephosphorylation by phosphatases. Hedgehog response elements (HRE) that interact with the dephosphorylated transcription factors are also provided as well as methods for identifying compounds that are phosphatase inhibitors. Methods of treating tumors in a ... 09/21/06 - 20060211113 - Method and system for introducing a gene into a human stem cell A method and vector system for delivering of a gene into a human stem cell for therapeutic uses is disclosed. The method includes linking a therapeutic gene to human sperm cells through a linker and fertilizing a human oocyte. The resulting zygote may then be cultured and established as human ... 09/14/06 - 20060205078 - Induction of rna interference by aberrant rna A method is provided for the induction of aberrant RNA interference (abRNAi) by introducing ‘aberrant’ RNAs into the cell wherein a long-stranded antisense or sense RNA is simultaneously introduced with a short 4-12 mer homologous or complementary or random RNA wherein the long-short RNAs induce sequence specific inhibition of the ... 09/14/06 - 20060205077 - Targeted transgenesis using the rosa26 locus The invention provides a method for targeted transgenesis using the Rosa26 locus. Suitable nucleotide acid sequences and vectors for the targeted transgenesis and recombinase mediated transgenesis are provided. The Rosa26 locus proved to be a suitable integration site allowing strong and predictable expression of inserted transgenes carrying exogenous promoters. ... 08/24/06 - 20060188989 - Inhibition of e3-ubiquitin ligase hakai for treatment of proliferative disorders Human HAKAI (hsHAKAI), an E3-ubiquitin ligase, can be inhibited to treat proliferative disorders, such as cancers, dysplasias and hyperplasias. Effective levels of hsHAKAI can be inhibited, for example, using antisense oligonucleotides, ribozymes, interference RNA, and antibodies. Test compounds can be screened for binding to hsHAKAI, for disruption of hsHAKAI-E-cadherin binding, ... 08/24/06 - 20060188988 - Optimized high yield synthetic plasmids One aspect of the current invention is an optimized synthetic mammalian expression plasmid with a mutated origin of replication (e.g. “mut” family of plasmids). This new plasmid comprises a therapeutic element, and a replication element. The therapeutic element of the new plasmid comprises a eukaryotic promoter; a 5′ untranslated region ... 08/24/06 - 20060188987 - Targeted deletion of cellular dna sequences Disclosed herein are methods and compositions for targeted deletion of double-stranded DNA. The compositions include fusion proteins comprising a cleavage domain (or cleavage half-domain) and an engineered zinc finger domain, and polynucleotides encoding same. Methods for targeted deletion include introduction of such fusion proteins, or polynucleotides encoding same, into a ... 08/24/06 - 20060188986 - Restoration of methylation states in cells A method for altering a characteristic or state of a cell, or reprogramming a cell, comprising treating a first cell type with an agent capable of altering a characteristic or state in a cell or reprogramming a cell, and determining the degree of alteration of the treated cell by measuring ... 08/17/06 - 20060183225 - Method of enhancing homologous recombination of somatic cells and method of constructing specific antibody The present invention provides a novel method for obtaining diverse antibodies as a result of markedly enhancing the somatic homologous recombination at an antibody locus in immunocytes. By putting immunocytes in which DNA homologous recombination is occurring at an antibody locus (for example, DT40 cells and the like) into contact ... 08/10/06 - 20060177931 - Apparatus and method for co-culturing of cells A co-culture apparatus and method for, among other things, converting a progenitor cell line into a target cell line. The apparatus comprises an exterior receptacle, a removable cartridge that fits into the exterior receptacle, and a closure member to seal the apparatus. The invention is also directed to a method ... 08/03/06 - 20060172420 - Gene trap system The present invention provides a gene-trap system, which can trap any gene including transiently expressed genes. A gene can be trapped by a trap vector, which employs Cre recombinase from bacteriophage P1, and the expression of the trapped gene is converted to constitutive expression of another reporter gene. ... 08/03/06 - 20060172419 - Highly efficient gene targeting vectors and methods for gene targeting method forward epithelial cell line In the process of generating ZO-1 gene knockout mice, the present inventors developed gene targeting vectors that allow homologous recombination to occur at a high probability (90% or more). In addition, the present inventors used the targeting vectors for studying optimal electroporation conditions in epithelial cells, and as a result, ... 08/03/06 - 20060172418 - Hypercoiling polymers and their use in cellular delivery This invention pertains to certain hypercoiling polymers, and their use for the delivery of a payload into a living cell, e.g., into the nucleus of a living cell, which polymer incorporates, or is otherwise associated with, said payload. The payload may be, for example, a therapeutic payload, such as a ... 07/27/06 - 20060166362 - Method of inducing differentiation of bone marrow stromal cells to neural cells or skeletal muscle cells by introduction of notch gene There is provided a method of inducing differentiation of bone marrow stromal cells to neural cells or skeletal muscle cells by introduction of a Notch gene. Specifically, the invention provides a method of inducing differentiation of bone marrow stromal cells to neural cells or skeletal muscle cells in vitro, which ... 07/20/06 - 20060160219 - Method of transferring mutation into target nucleic acid A method of transferring a mutation into the base sequence of a target nucleic acid characterized by comprising the step of preparing a DNA having a reversed repetitive sequence wherein the base sequence of the DNA having the reversed repetitive sequence is homologous with the target nucleic acid and has ... 07/20/06 - 20060160218 - Transdifferentiation of cells and tissues Method for carrying out transdifferentiation of non-pancreatic cells to pancreatic cells involving the provision of a pancreas specific transcription factor and an activating means such as VP16 of Herpes simplex virus to non-pancreatic cells. The methods and materials provided by the invention may be used to treat pancreatic disorders, in ... 07/13/06 - 20060154368 - Transgenic mice containing cx2 gene disruptions The present invention relates to transgenic animals, as well as compositions and methods relating to the characterization of gene function. Specifically, the present invention provides transgenic mice comprising mutations in a CX2 gene. Such transgenic mice are useful as models for disease and for identifying agents that modulate gene expression ... 07/06/06 - 20060148086 - Vault and vault-like carrier molecules A method of using vaults as carrier molecules to deliver one or more than one substance to an organism, or to a specific tissue or to specific cells, or to an environmental medium. A vault-like particle. A method of preventing damage by one or more than one substance to an ... 07/06/06 - 20060148085 - High yield heterologous expression cell lines for expression of gene products with human glycosylation pattern The invention relates to ubiquitous/universal processes for establishing cells capable of stable high yield expression of a recombinant gene with human glycosylation pattern, and for establishing stable universal precursor cells available for insertion of arbitrary target genes. The invention further relates to cells obtainable by said processes ... 06/29/06 - 20060141626 - Non-random method of gene shuffling The present invention concerns the non-random assembling of DNA molecules in a DNA construct and methods of using such constructs, including the production of nucleic acid libraries. The non-random gene shuffling is preferably accomplished by the following steps. First, optionally, the amino acid sequences of proteins encoded by related gene ... 06/29/06 - 20060141625 - Expression cassette and vector for transient or stable expression of exogenous molecules The disclosure provides an expression cassette and a vector comprising the cassette for expression of a polynucleotide. The expression cassette includes a promoter/enhancer, an intervening region, and a polyadenylation signal domain. Expression systems and methods of using the expression cassette and vector are also provided. ... 06/22/06 - 20060134790 - Solid surface with immobilized degradable cationic polymer for transfecting eukaryotic cells A cell transfection/culture device is disclosed which includes a solid support coated with a degradable polymer cation as a transfection reagent. The transfection/culture device is conveniently stored at room temperature until use. Cell transfection is accomplished easily by adding the nucleic acid of interest and the cells to be transfected ... 06/22/06 - 20060134789 - Methods and products for biasing cellular development Methods are described that bias cells, such as potent and multipotent stem cells, by transfection with a nucleic acid sequence, to differentiate to a desired end-stage cell or a cell having characteristics of a desired end-stage cell. In particular embodiments, human neural stem cells are transfected with vectors comprising genes ... 06/22/06 - 20060134788 - Recombinant vector containing mj1 gene and method of site-specific integration using the same The present invention relates to a recombinant vector comprising MJ1 gene coding an integrase derived from Enterococcus temperate bacteriophage φ FC1 and an integration method using the vector. More particularly, the present invention relates to a recombinant vector comprising MJ1 gene which can make a site-specific integration in the human ... 06/22/06 - 20060134787 - Methods and compositions for enhancing the efficacy and specificity of single and double blunt-ended sirna The present invention provides methods of enhancing the efficacy and specificity of RNAi using single or double blunt-ended siRNA. The invention also provides single and double-blunt ended siRNA compositions, vectors, and transgenes containing the same for mediating silencing of a target gene. Therapeutic methods are also featured. ... 06/15/06 - 20060128018 - Directed genetic modifications of human stem cells Human embryonic stem cells can be genetically transformed by a combination of electroporation and homologous recombination. This technique makes it possible to create targeted inserts or deletions to the genome of the stem cells. This ability makes it possible to create populations of progeny cells which have differentiated into a ... 06/15/06 - 20060128017 - Process for the production of an active molecule vector used to diffuse active substances and vector thus obtained Adding glutaraldehyde, OHC—(CH2)3—COH, and Awaiting the polycondensation reaction and the formation of imines, and Recovering the poly(monomer-G) that is obtained. The monomer is selected from among the L-ornithine, the L-lysine or the L-citrulline. Further described are the biomedical vector that is obtained, and the use as a vector of active ... 05/25/06 - 20060110828 - Compositions and methods for selection of a pure population of cells from a mixed population Disclosed are methods and compositions for deriving a pure population of differentiated cells from a stem cell. The method comprises transforming a stem cell with a gene construct containing a selectable marker under the control of a tissue- or cell-type-specific regulatory element, such that the gene construct integrates at a ... 05/18/06 - 20060105459 - Transgenic mice containing nttp1 phosphatase gene disruptions The present invention relates to transgenic animals, as well as compositions and methods relating to the characterization of gene function. Specifically, the present invention provides transgenic mice comprising mutations in a NTTP1 gene. Such transgenic mice are useful as models for disease and for identifying agents that modulate gene expression ... 05/18/06 - 20060105458 - Caged tetracycline (derivatives), their generation, and their use for photoactivated gene expression Transcriptionally inactivated (caged) tetracycline or tetracycline derivative, wherein the inactivation is caused by reaction of the tetracycline (derivative) with a photosensitive protection compound, said caged tetracycline or tetracycline derivative being capable to be activated again by photolysis. ... 05/04/06 - 20060094115 - Method for the production of cells with increased development potential The invention relates to a method for producing cells having an increased development potential from cells, which are removed from an organism, wherein the removed cells are cultivated in vitro, and wherein a native nuclear non-activated MAPKAP kinase of the cultivated cell is activated or wherein an activated MAPKAP kinase ... 04/20/06 - 20060084172 - Method for producing stem cells with increased developmental potential The invention relates to a method for producing stem cells having an increased development potential from somatic stem cells, wherein a tissue sample comprising somatic stem cells or a body fluid sample comprising somatic stem cells is taken from an organism, wherein from this tissue sample or body fluid sample ... 04/13/06 - 20060078996 - Surface transfection and expression procedure The present invention relates to a method of transfecting cells comprising applying cells directly onto nucleic acids which are immobilized in transfection complexes on a surface and which transfect the cells. Preferably, the nucleic acids are immobilized in an array. In another aspect of the present invention, the method further ... 04/06/06 - 20060073593 - Compositions and methods for molecular biology The present invention provides materials and methods for the utilization of the specific interaction of replication termination sequences with their binding proteins in molecular biology applications. ... 03/30/06 - 20060068497 - Methods for isolating novel antimicrobial agents from hypermutable mammalian cells Dominant-negative alleles of human mismatch repair genes can be used to generate hypermutable cells and organisms. By introducing these genes into mammalian cells new cell lines with novel and useful properties can be prepared more efficiently than by relying on the natural rate of mutation or introduction of mutations by ... 03/30/06 - 20060068496 - Differentiation of stem cells Disclosed are compositions and methods for identifying specific cell types. ... 03/23/06 - 20060063257 - Formation of polyampholytes in the presence of a polyion Polyampholyte are able to condense nucleic acid to form small complexes which can be utilized in the delivery of nucleic acid to mammalian cells. The polyampholytes can be formed prior to interaction with nucleic acid or they can be formed in the presence of nucleic acid. Stabilized polycation/nucleic acid complexes ... 03/09/06 - 20060051869 - Transport proteins and their uses The present invention relates to transport proteins, in particular VP22 and homologues thereof, and to methods of delivering these proteins and any associated molecules to a target population of cells. This transport protein has applications in gene therapy and methods of targeting agents to cells where targeting at high efficiency ... 02/23/06 - 20060040392 - Multi-lineage progenitor cells Fetal blood multi-lineage progenitor cells that are capable of a wide spectrum of transdifferentiation are described. ... 02/23/06 - 20060040391 - Rna interference vectors The present invention relates to gene-specific silencing through RNA interference, and in particular, to vectors for expressing RNAi molecules. In some embodiments, the present invention provides compositions and methods for inducible expression of RNAi molecules, and/or for long-term expression of RNAi molecules. Hence the compositions and methods described herein are ... 02/23/06 - 20060040390 - Device, method, system, and program for intelligent in vivo cell-level chemical or genetic material delivery The invention provides a device, method, system and program for intelligent in vivo cell-level chemical or genetic material delivery; wherein multiple injectable biocompatible physical delivery device containers are used to selectively administer medicine, chemical(s) or genetic materials to a target cell in a patient, human or animal, with reduced systemic ... 02/02/06 - 20060024829 - Adenovirus packaging cell lines Adenovirus packaging cell lines for growth of an E1A/E1B deficient adenovirus that is substantially free of replication competent adenovirus (RCA) are provided. Methods for producing adenovirus substantially free of RCA are also provided, wherein the adenovirus is grown in a cell line containing coding sequences for adenovirus E1A and E1B, ... 02/02/06 - 20060024828 - Process of making a compound by forming a polymer from a template drug A method of forming polymers in the presence of nucleic acid using template polymerization. Also, a method of having the polymerization occur in heterophase systems. These methods can be used for the delivery of nucleic acids, for condensing the nucleic acid, for forming nucleic acid binding polymers, for forming supramolecular ... 01/26/06 - 20060019392 - Novel bone mineralization proteins, dna, vectors, expression systems The present invention is directed to isolated nucleic acid molecules that encode LIM mineralization protein, or LMP. The invention further provides vectors comprising nucleotide sequences that encode LMP, as well as host cells comprising those vectors. Moreover, the present invention relates to methods of inducing bone formation by transfecting osteogenic ... 01/19/06 - 20060014287 - Isolation and characterization of muscle regenerating cells Populations enriched for myogenic progenitors are obtained by selection on the basis of expression of specific cell surface markers. The muscle progenitor cells are characterized as being CD45− and CD34+, and may further be characterized as lacking expression of Mac-1 (CD11b) and positive for expression of CXCR4 (CD184) and β1-integrin ... 01/19/06 - 20060014286 - Ligand-controlled regulation of endogenous gene expression Disclosed herein are compositions comprising an engineered zinc finger protein and a nuclear hormone receptor transcriptional control domain, polynucleotides encoding one or more components of the compositions, methods of making the compositions and methods of using these compositions, for example to modulate gene expression and/or to generate transgenic plants or ... 01/12/06 - 20060008908 - Method and composition for longevity assurance This present invention provides compositions and methods for Longevity Assurance and treatment of disorders associated with age-related diseases. The compositions and methods further address age-related or incorrect or abnormal regulation of cellular homeostasis by controlling mechanisms of systemic, intracellular and extracellular ionic physiology through the administration of alkaline salts. The ... 01/12/06 - 20060008907 - Control of gene expression via light activated rna interference A method for controlling the spacing, timing and degree of gene expression that includes selecting a target mRNA, obtaining siRNA corresponding to the target mRNA, modifying the siRNA with a photo-labile group to inhibit RNA interference, introducing the modified siRNA into a cell, and selectively irradiating the cell with light ... 01/05/06 - 20060003450 - Lineage specific cells and phogenitor cells A method for generating a culture that is purified or enriched in respect of cells of a selected lineage is described in which a selectable marker, which is differentially expressed in cells of the selected lineage compared with its expression in other cells, is introduced into a multipotential cell and ... 12/29/05 - 20050287670 - Cell culturing systems, methods and apparatus The present disclosure provides cell-culturing methods, apparatus and systems wherein cells in cell cultures are subjected to novel shear forces, which provide improved and efficient target product production. The shear force is provided by a reactor apparatus connected to a cell culture apparatus by a pump for transporting said cell ... 12/29/05 - 20050287669 - Nucleic acid construct and expression vector for enhancing the production of recombinant protein, and method for the massive production of recombinant protein Disclosed herein are nucleic acid constructs and expression vectors for enhancing the production of recombinant polypeptides/proteins, and methods for the massive production of recombinant polypeptides/proteins, in which a first nucleic acid sequence encoding thioredoxin and a second nucleic acid sequence encoding hemoglobin are cloned into a host cell, thereby enhancing ... 12/29/05 - 20050287668 - Rna interference compositions and screening methods for the identification of novel genes and biological pathways The present invention provides compositions and methods for enhancing RNA interference and facilitating the use of long RNA interference molecules. Accordingly, the invention includes a variety of novel applications of RNA interference, including methods related to screening RNA interference molecules using reporter genes to identify biological pathways, genes, therapeutic compounds ... 12/29/05 - 20050287667 - Methods and compositions for the inhibition of gene expression The present invention relates to methods and compositions for the inhibition of gene expression. In particular, the present invention provides oligonucleotide-based therapeutics for the inhibition of oncogenes involved in cancers. ... 12/08/05 - 20050272154 - Gene expression with covalently modified polynucleotides A process and compound wherein nucleic acids can be modified with a host of molecules and maintain their ability to be expressed. A modifying chemical attachment of polyions to polynucleotides can be used to facilitate the change of tertiary structure of the nucleic acid and in some cases condensation of ... 12/01/05 - 20050266563 - Methods and compositions relating to neuronal cell and tissue differentiation The invention relates to methods for isolating and purifying specific types of neurons, such as cortical or other projection neurons including corticospinal motor neurons, subcerebral projection neurons, and callosal projection neurons. The invention also relates to genes that are specific for particular neuronal subtypes, and the use of such genes ... 12/01/05 - 20050266562 - Myeloid cell promoter and constructs containing same The present invention provides novel myeloid cell specific promoters, and cis-acting elements that influence the activity of a myeloid cell specific promoters, as well as promoter-heterologous gene constructs and transfected myeloid cells which include the novel promoters and/or the novel cis-acting elements. The present invention also provides a method of ... 12/01/05 - 20050266561 - Use of interfering rna in the production of transgenic animals The invention provides cells and animals, as well as methods of producing cells and animals, that express at least one interfering RNA molecule to regulate the expression of a specific gene or family of genes. The invention further provides novel iRNA molecules, as well as DNA templates for producing iRNA ... 11/24/05 - 20050260755 - Sequential delivery of oligomeric compounds The present invention provides double stranded compositions that have a region that is complementary to a target nucleic acid. The targeting strand comprises linked ribofuranosyl nucleosides and the second strand comprises linked modified nucleosides that have 3′-endo conformational geometry. The strands can be linked together or separate and may contain ... 11/24/05 - 20050260754 - Constructs and methods for the regulation of gene expression The present invention relates to constructs and methods for regulating the gene expression of at least two endogenous target genes by introducing, into a eukaryotic cell or a eukaryotic organism, an at least partially double-stranded ribonucleic acid molecule, the ribonucleic acid molecule comprising at least two ribonucleotide sequence segments which ... 11/17/05 - 20050255596 - Methods of repairing tandemly repeated dna sequences and extending cell life-span nuclear transfer This invention relates to methods for rejuvenating normal somatic cells and for making normal somatic cells of a different type having the same genotype as a normal somatic cell of interest. These cells have particular application in cell and tissue transplantation. Also encompassed are methods of re-cloning cloned animals, particularly ... 11/17/05 - 20050255595 - Genetically modified non-human mammal cells, procedure for their production and use in toxicity tests The invention concerns cells that can be used in toxicity tests and which therefore secrete, in response to toxic stress and stimuli, products that can be monitored and quantified. According to the present invention genetically modified cells are produced via the introduction of a first exogenous gene codifying a marker ... 11/03/05 - 20050244970 - Stem cell libraries A stem cell library is created by genetically modifying stem cells with nucleic acids encoding polypeptides which can promote stem cell differentiation into specific cell types. Alternatively, the stem cell library is exposed to an externally added factor that promotes stem cell differentiation into a desired cell line, e.g., neuronal ... 11/03/05 - 20050244969 - Transient immortalization The invention relates to a method for transiently immortalizing cells in which immortalizing proteins are introduced into the cells from the exterior, and to a method for obtaining cells in which organ-related cells are transiently immortalized by supplying immortalizing proteins externally and are remortalized after expansion. The invention furthermore relates ... 10/27/05 - 20050239204 - Multifunctional molecular complexes for gene transfer to cells A multifunctional molecular complex for the transfer of a nucleic acid composition to a target cell is provided. The complex is comprised of A) said nucleic acid composition and B) a transfer moiety comprising 1) one or more cationic polyamines bound to said nucleic acid compositions, 2) one or more ... 10/27/05 - 20050239203 - Regulation of endogenous gene expression in cells using zinc finger proteins The present invention provides methods for modulating expression of endogenous cellular genes using engineered zinc finger proteins. ... 10/27/05 - 20050239202 - Methods and compositions for inhibiting the function of polynucleotide sequences A therapeutic composition for inhibiting the function of a target polynucleotide sequence in a mammalian cell includes an agent that provides to a mammalian cell an at least partially double-stranded RNA molecule comprising a polynucleotide sequence of at least about 200 nucleotides in length, said polynucleotide sequence being substantially homologous ... 10/20/05 - 20050233455 - Acyclic linker-containing oligonucleotides and uses thereof Oligonucleotides having an internal acyclic linker residue, and the preparation and uses thereof, are described. Such uses include the preparation of acyclic linker-containing antisense oligonucleotides, and their use for the prevention or depletion of function of a target nucleic acid of interest, such as RNA, in a system. Such a ... 10/06/05 - 20050221488 - Methods for inducing targeted stimulation of meiotic recombination and kits for performing said methods The Invention pertains to methods, kits, molecules and cells to increase the rate or recombination and/or target recombination in dividing cells. In a particular aspect, the invention concerns methods and kits to induce targeted meiotic recombination. ... 10/06/05 - 20050221487 - Method of enhancing proliferation and/or hematopoietic differentiation of stem cells The present invention provides a method for enhancing the proliferation and/or hematopoietic differentiation and/or maintenance of mammalian stem cells. The method is useful for generating expanded populations of hematopoietic stem cells (HSCs) and thus mature blood cell lineages. This is desirable where a mammal has suffered a decrease in hematopoietic ... 09/29/05 - 20050214944 - In vivo homologous sequence targeting in cells The invention relates to methods for targeting an exogenous polynucleotide or exogenous complementary polynucleotide pair to a predetermined endogenous DNA target sequence in a target cell by homologous pairing, particularly for altering an endogenous DNA sequence, such as a chromosomal DNA sequence, typically by targeted homologous recombination. In certain embodiments, ... 09/29/05 - 20050214943 - Gene regulatory peptides The invention relates to the modulation of gene expression in a cell, also called gene control, in particular in relation to the treatment of a variety of diseases. The invention provides a method for modulating expression of a gene in a cell comprising providing the cell with a signaling molecule ... 09/22/05 - 20050208658 - Rna interference mediated inhibition of 11beta hydroxysteriod dehydrogenase-1 (11beta hsd-1) gene expression The present invention relates to compositions comprising double stranded RNA capable of inhibiting the expression of the gene encoding 11β HSD-1, and methods of using the compositions in therapeutic, prophylactic, and research methods. ... 09/22/05 - 20050208657 - Delivery of functional protein sequences by translocating polypeptides The invention provides methods for modulating a cellular process by contacting a cell in culture with a cell process-modifying molecule attached to a translocating polypeptide. For example, in one embodiment, a cell in culture is transfected with a target gene by contacting the cell in culture with a polynucleotide (that ... 09/15/05 - 20050202559 - Cancer treatment by metabolic modulations The invention provides compositions and methods for inhibiting the growth or proliferation of hyperproliferative cells or inducing regression of hyperproliferative cells. More specifically, the invention provides compositions and methods for stimulating glycogen accumulation in target cells (e.g., hyperproliferative cells) in order to increase glycogen to a level that is toxic ... 09/08/05 - 20050196863 - Pyridinium cationic lipids as gene transfer agents Pyridinium cationic lipids useful as non-viral gene delivery agents are disclosed. The agents are prepared by reaction of pyrylium salts with primary amines. Also disclosed are methods of trasfectind cells using the pyridinium cationic lipids as gene transfer agents. ... 09/08/05 - 20050196862 - Dna cassette for cellular expression of small rna We describe expression cassettes and processes for preparing expression cassettes that can be delivered to animal cells in vivo or in vitro. Delivery of the cassettes results in expression of small RNA transcripts such as siRNA in the cell. The cassettes can by used to inhibit gene expression in the ... 09/01/05 - 20050191746 - Compositions and methods for biodegradable polymer-peptide mediated transfection Complexes are disclosed which are a combination of a polynucleotide and a biodegradable polyacetal-peptide. The disclosed complexes are useful for cell transfection. ... 08/25/05 - 20050186676 - Selective cloning of homoduplex nucleic acids The subject invention provides for a method of selectively cloning homoduplex nucleic acid molecules, in particular, by using a strain of host cells that contains a conditionally expressed and/or conditionally active mismatch-recognizing enzyme, e.g., a temperature sensitive variant of the gene encoding the endonuclease VII from phage T4. Using this ... 08/25/05 - 20050186675 - Allelic series of genomic modifications in cells The present invention relates to methods of producing an allelic series of modifications in genes of interest in a cell. In particular, the invention provides methods for using nucleic acid sequence-modifying agents (e.g., chemicals, electromagnetic radiation, etc.) to introduce modifications in any nucleic acid sequence in the genome of a ... 08/18/05 - 20050181506 - Chromosome-based platforms Artificial chromosomes, including ACes, that have been engineered to contain available sites for site-specific, recombination-directed integration of DNA of interest are provided. These artificial chromosomes permit tractable, efficient, rational engineering of the chromosome for a variety of applications. ... 08/11/05 - 20050176145 - Animal integration vector and methods for its use Novel P element containing vectors and methods for their use to insert an exogenous nucleic acid into the genome of an animal are provided. The subject vectors have a pair of P element transposase recognized insertion sites, e.g. 31 base pair inverted repeats, flanking at least one transcriptionally active gene ... 08/04/05 - 20050170506 - Therapeutic reprogramming, hybrid stem cells and maturation Therapeutically programmed cells and methods for making such cells are provided. Therapeutically programmed cells are stem cells which have been matured such that they represent either a more differentiated state or a less differentiated state after contact with stimulatory factors. The therapeutically reprogrammed cells are suitable for cellular regenerative therapy ... 08/04/05 - 20050170505 - Formation of polyampholytes in the presence of a polyion Polyampholyte are able to condense nucleic acid to form small complexes which can be utilized in the delivery of nucleic acid to mammalian cells. The polyampholytes can be formed prior to interaction with nucleic acid or they can be formed in the presence of nucleic acid. Stabilized polycation/nucleic acid complexes ... 08/04/05 - 20050170504 - Method for transfer of molecular substances with prokaryontic nucleic acid-binding proteins The invention relates to a method for the transfer of molecular substances, for example proteins or nucleic acids in cells, in the case of using DNA combined with a possible gene expression. A prokaryotic nucleic acid-binding protein is used for the transfer, which is preferably obtained from a thermostable organism. ... 07/28/05 - 20050164391 - Transfection reagents Q is selected from the group consisting of N, O and S; L is any bivalent organic radical capable of linking each Q, such as C, CH, (CH2)l, or {(CH2)i-Y—(CH2)j}k, wherein Y is selected from the group consisting of CH2, an ether, a polyether, an amide, a polyamide, an ester, ... 07/21/05 - 20050158859 - Manipulation of non-terminally differentiated cells using the notch pathway The present invention is directed to methods for the expansion of non-terminally differentiated cells (“precursor cells”) using agonists of Notch function, by inhibiting the differentiation of the cells without inhibiting proliferation (mitotic activity) such that an expanded population of non-terminally differentiated cells is obtained. The cells are preferably stem or ... 07/07/05 - 20050148075 - Zinc finger binding domains for gnn Zinc finger-nucleotide binding polypeptides having binding specificity for target nucleotides containing one or GNN triplets are provided. Compositions containing such polypeptides and the use of such polypeptides and compositions for regulating gene expression are also provided. ... 06/09/05 - 20050124067 - Novel attached cell lines The present invention provides novel cell lines that may have improved adhesive qualities, transgene expression level, growth rate, and/or growth rate in serum free medium or even chemically defined compared to cells of the prior art. ... 06/09/05 - 20050124066 - Method for preparing polypeptides variants The present invention relates to a method for preparing positive polypeptide variants by shuffling different nucleotide sequences of homologous DNA sequences by in vivo recombination comprising the steps of (a) forming at least one circular plasmid comprising a DNA sequence encoding a polypeptide, (b) opening said circular plasmid(s) within the ... ### FreshPatents.com Support |