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Chemistry: Molecular Biology And Microbiology > Animal Cell, Per Se (e.g., Cell Lines, Etc.); Composition Thereof; Process Of Propagating, Maintaining Or Preserving An Animal Cell Or Composition Thereof; Process Of Isolating Or Separating An Animal Cell Or Composition Thereof; Process Of Preparing A Composition Containing An Animal Cell; Culture Media Therefore > Primate Cell, Per Se > Human HumanHuman patent applications listed are from June 2005 to current and include Date, Patent Application Number, Patent Title, Patent Abstract summary and are linked to the corresponding patent application page.04/19/07 - 20070087435 - Multicellular compositions of pluripotent human embryonic stem cells and cancer cells Methods are provided for producing novel multicellular compositions comprising cancer cells together with pluripotent human stem cells, which are capable of proliferating and differentiating into various normal cell lines and tissue structures. These novel multicellular compositions are useful for investigating the properties of cancer cells in a normal human tissue ... 04/12/07 - 20070082397 - Ex vivo progenitor and stem cell expansion for use in the treatment of disease of endodermally-derived organs Methods of ex-vivo expansion of endodermally-derived and non-endodermally-derived progenitor and stem cells, expanded populations of renewable progenitor and stem cells and to their uses in therapeutic applications such as the production of endocrine hormones and the prevention and treatment of liver and pancreatic disease. ... 04/05/07 - 20070077653 - Modulation of cell intrinsic strain to control matrix synthesis, secretion, organization and remodeling The present invention provides methods for manipulating the intrinsic strain of cells by treating tissue engineered constructs or native tissue with compounds which affect the intrinsic strain setpoint of the cells in order to modulate matrix synthesis, secretion, organization and/or remodeling so that the tissues withstand in vivo mechanical forces ... 03/15/07 - 20070059822 - Method for isolating cells from mammary secretion The present invention relates to a method for isolating progenitor cells from a human body, inclusive of all cells with stem cell-like characteristics, in particular pluripotent or multipotent progenitor cells, wherein such cells are directly or indirectly derived from human mammary secretion, be it colostrum, mature milk, or dry period ... 03/08/07 - 20070054398 - Human salivary gland-origin stem cell A novel human stem cell which can be differentiated to cells constituting a plurality of human organs including human liver is disclosed. The human stem cell according to the present invention is originated from human salivary gland, which is CD49f-positive, and which can be differentiated to (1) a nestin-positive and ... 03/01/07 - 20070048864 - Isolation of inner cell mass for the establishment of human embryonic stem cell (hesc) lines A method for isolating an inner cell mass comprising the steps of immobilizing a blastocyst stage embryo having a zona pellucida, trophectoderm, and inner cell mass, creating an aperture in the blastocyst stage embryo by laser ablation, and removing the inner cell mass from the blastocyst stage embryo through the ... 02/22/07 - 20070042491 - Amplification of cell populations from embryonic stem cells Culturing embryonic stem cells without the use of embryoid bodies leads to a increase in the frequency of predetermined cell types. ... 02/15/07 - 20070037281 - Method for differentiating stem cells in cells that produce a pancreatic hormone Described is a process for generating cells producing pancreatic hormone by cultivating and differentiating stem cells obtained from differentiated exocrine glandular tissue of an organism. The use of the cells producing pancreatic hormone is also described. ... 02/01/07 - 20070026520 - Novel cells, compositions, and methods Disclosed are compositions and methods for producing cells and stem cells. ... 01/25/07 - 20070020759 - Therapeutic reprogramming of germ line stem cells Pluripotent therapeutically programmed cells and methods for making such cells are provided. The pluripotent therapeutically programmed cells are post-natal stem cells which have been matured such that they represent either a more differentiated state or a less differentiated state after contact with stimulatory factors. The pluripotent therapeutically reprogrammed cells are ... 01/25/07 - 20070020758 - Method for the isolation and expansion of cardiac stem cells from biopsy Method for the isolation, expansion and preservation of cardiac stem cells from human or animal tissue biopsy samples to be employed in cell transplantation and functional repair of the myocardium or other organs. Cells may also be used in gene therapy for treating genetic cardiomyopathies, for treating ischemic heart diseases ... 01/18/07 - 20070015279 - Methods of selecting pancreatic endocrine cells using protein synthesis inhibitors The present invention relates to methods of selecting pancreatic endocrine cells from total pancreatic cells by incubation with protein synthesis inhibitors. ... 01/18/07 - 20070015278 - Methods of isolating differentiable cells from placental-associated tissue and uses thereof The invention relates to methods of isolating differentiable fetal-derived cells from various tissues of the placenta. Specifically, some of the methods of the present invention relate to isolating differentiable amnion-derived cells, while other methods of the present invention relate to isolating differentiable chorion-derived cells. The present invention also relates to ... 01/11/07 - 20070010013 - Oocytes derived from ovarian culture initially containing no oocytes Ovarian germ-line-competent embryonic stem cells (GLC-ESC) are cultured, either in the presence or absence of a compound having estrogenic activity. The GLC-ESC are either collected prior to specific commitment or are permitted to remain in the culture medium for a time sufficient to develop into oocytes, and the oocytes may ... 01/11/07 - 20070010012 - Differentiation of primate pluripotent stem cells to cardiomyocyte-lineage cells The present application describes the new methods for the differentiation of primate pluripotent stem cells into cardiomyocyte-lineage cells. The methods utilize sequential culturing of the primate pluripotent stem cells in certain growth factors to produce cardiomyocyte-lineage cells. In certain embodiments of the invention, the population of cells produced by the ... 01/11/07 - 20070010011 - Defined media for pluripotent stem cell culture Stem cells, including mammalian, and particularly primate primordial stem cells (pPSCs) such as human embryonic stem cells (hESCs), hold great promise for restoring cell, tissue, and organ function. However, cultivation of stem cells, particularly undifferentiated hESCs, in serum-free, feeder-free, and conditioned-medium-free conditions remains crucial for large-scale, uniform production of pluripotent ... 01/11/07 - 20070010010 - Method for efficient transfer of human blastocyst-derived stem cell (hbs cells) from a feeder-supported to a feeder-free culture system A method for the transfer of human blastocyst-derived stem cells (hBS cells) to feeder-free culture system and propagation of the cells in such a feeder-free culture system, the method comprising the following steps of (a) transferring the balstocyst-derived stem cells from feeder to feeder free culture by mechanical treatment, (b) ... 01/04/07 - 20070004038 - Preprimitive streak and mesendoderm cells This disclosure relates to compositions comprising human preprimitive streak cells and/or human mesendoderm cells as well as methods for their production. Additionally, disclosed herein are methods of identifying factors useful in the further differentiation of preprimitive streak and mesendoderm cell types. ... 12/07/06 - 20060275900 - Methods for in vitro expansion and transdifferentiation of human pancreatic acinar cells into insulin-producing cells This invention relates, e.g., to a method for expanding mammalian acinar cells, comprising culturing the cells in a cell culture system comprising a cell culture medium and a cell attachment surface, under conditions wherein the acinar cells undergo a 3-4 fold expansion together with transdifferentiation into a modified cell phenotype ... 12/07/06 - 20060275899 - Methods and compositions relating to embryonic stem cell lines The invention provides methods for generating customized hESC and lines thereof for future use in the treatment of genetic relations. In one aspect, the customized hESC lines are grown using human feeder cells or human serum from biological or genetic relations in order to reduce or preferably avoid contamination by ... 11/23/06 - 20060263881 - Methods of expanding and selecting disease associated t-cells Methods of expanding and selecting disease associated T-cells, continuous T-cell lines as well as T-cell lines obtainable by these methods are disclosed. Furthermore, pharmaceutical compositions and vaccines comprising activated disease associated T-cell are disclosed. The uses of the T-cell and T-cell lines are numerous and include methods of diagnosis, methods ... 11/23/06 - 20060263880 - Protein factors supporting undifferentiated growth of human embryonic stem cells Factors which are secreted by mouse embryonic fibroblasts which promote maintenance and support the growth of human embryonic stem (hES) cells are provided. The factors can be isolated by chromatographic techniques. The genes, cDNA and amino acid sequences for the factors are disclosed herein and identified. The cDNA of one ... 11/23/06 - 20060263879 - Methods and systems relating to embryonic stem cell lines The invention provides methods for generating customized hESC and lines thereof for future use in the treatment of genetic relations. In one aspect, the customized hESC lines are grown using feeder cells from biologically or genetic relations in order to avoid contamination by foreign pathogens or exposure to foreign antigens. ... 11/23/06 - 20060263878 - Cell culture medium and solidified preparation of cell adhesion protein or peptide The present invention provides a cell culture substrate which effectively adsorbs to a cell culture substrate such as a well, which is excellent in cell adhesion reproducibility, and whose surface is coated with a hydrophobic binding-adsorptive polymer and an immobilized preparation of cell adhesion proteins or peptides which effectively binds ... 11/16/06 - 20060258003 - Culture medium composition, culture method, and myoblasts obtained, and their uses The invention concerns a composition of culture medium of progenitor/stem cells derived from muscular tissues containing serum and/or serum fraction of human origin and/or of animal origin of insulin or a derivative thereof, and one or several compound(s) selected among the class of antioxidants and/or of vitamins. The invention also ... 11/09/06 - 20060252148 - Container for germ layer formation and method of forming germ layer The invention relates to a vessel for embryoid formation used for forming embryoid bodies from ES cells easily without complicated technique, and to a method for forming embryoid bodies easily and efficiently using the vessel. The method includes the steps of (A) providing a vessel for embryoid formation having a ... 11/02/06 - 20060246583 - Spatial control of signal transduction Substrates and matrices for spatial control of signal transduction by a biologically active, soluble molecule that transduces a signal to a cell responsive to the molecule are disclosed. ... 11/02/06 - 20060246582 - Inhibition of pancreatic islet aggregation A method for improving the health and viability of pancreatic islets and improving the outcome of pancreatic islet transplantations is described. The method includes incubating the islets with an IKVAV-containing laminin A chain peptide, such as PA22-2, either before or during the culturing of the islets in an RCCS bioreactor. ... 10/19/06 - 20060234376 - Repair and regeneration of ocular tissue using postpartum-derived cells Cells derived from postpartum umbilicus and placenta are disclosed. Pharmaceutical compositions, devices and methods for the regeneration or repair of ocular tissue using the postpartum-derived cells are also disclosed. ... 10/19/06 - 20060234375 - Use of human stem cells and/or factors they produce to promote adult mammalian cardiac repair through cardiomyocyte cell division A method for treating a subject afflicted with a cardiac disorder, in vivo, comprising (i) producing a solution comprising media conditioned from the culture of cells, in vitro, and (ii) administering the solution of step (i) to the subject, thereby treating the cardiac disorder in the subject. Methods for determining ... 10/05/06 - 20060223180 - Methods to mobilize progenitor/stem cells wherein each R is independently H or straight, branched or cyclic alkyl (1-6C), n is 1 or 2, and X is an aromatic ring, including heteroaromatic rings, or is a mercaptan; “linker” represents a bond, alkylene (1-6C) or may comprise aryl, fused aryl, oxygen atoms contained in an alkylene chain, ... 09/21/06 - 20060211110 - Human trophoblast stem cells and use thereof Existence of human trophoblast stem (hTS) cells has been suspected but unproved. The isolation of hTS cells is reported in the early stage of chorionic villi by expressions of FGF4, fgfr-2, Oct4, Thy-1, and stage-specific embryonic antigens distributed in different compartments of the cell. hTS cells are able to derive ... 09/21/06 - 20060211109 - Derivation of terminally differentiated dopaminergic neurons from human embryonic stem cells The present disclosure is directed to improved methods for efficiently producing neuroprogenitor cells and differentiated neural cells such as dopaminergic neurons and serotonergic neurons from pluripotent stem cells, for example human embryonic stem cells. Using the disclosed methods, cell populations containing a high proportion of cells positive for tyrosine hydroxylase, ... 09/14/06 - 20060205073 - Aspirating oocytes from transplanted ovarian tissue A method for aspirating an oocyte from a human female is provided. The method includes the step of providing at least one ovarian cortical piece, implanting the ovarian cortical piece in the human female at a heterotopic location, and triggering oocyte maturity in the at least one ovarian cortical piece. ... 09/14/06 - 20060205072 - Enriched pancreatic stem cell and progenitor cell populations, and methods for identifying, isolating and enriching for such populations Enriched pancreatic stem and progenitor cell populations, and methods for identifying, isolating and enriching for pancreatic stem cells using reagents that bind to cell surface markers are provided. ... 09/14/06 - 20060205071 - Methods for ex-vivo expanding stem/progenitor cells Methods of ex-vivo expansion of fetal and/or adult progenitor, and umbilical cord blood, bone marrow or peripheral blood derived stem cells in bioreactors for bone marrow transplantation, transfusion medicine, regenerative medicine and gene therapy. ... 09/07/06 - 20060199263 - Method of isolating cells from umbilical cord The present invention relates a method for isolating four cell types from a single umbilical cord as pure cultures. These cell lines (epithelial cells, fibroblasts, smooth muscle cells and endothelial cells) can be characterised and utilised in experimental models and for therapeutic purposes. Particularly, the umbilical cells isolated herein are ... 08/31/06 - 20060194315 - Compositions and methods for the control, differentiaton and/or manipulation of pluripotent cells through a gamma-secretase signaling pathway The current invention relates to the control and/or manipulation of the gamma-secretase signaling pathway in pluripotent cells to stabilize the cells in a pluripotent state and/or to control the differentiation of the pluripotent cells towards a differentiated state. The invention further includes feeder layers that contain or express ligands or ... 08/24/06 - 20060188984 - Method of providing readily available cellular material derived from cord blood, and a composition thereof The present invention is directed to the TVEMF-expansion of mammalian cord blood stem cells, preferably CD34+/CD38− cells, to compositions resulting from the TVEMF-expanded cells, and to a method of treating disease or repairing tissue with the compositions. Various benefits and advantages to the compositions of the present invention are discussed ... 08/24/06 - 20060188983 - Postpartum-derived cells for use in treatment of disease of the heart and circulatory system Cells derived from postpartum tissue are disclosed along with methods for their therapeutic use in diseases of the heart or circulatory system are disclosed. Cells may be used therapeuticall in either differentiated or undifferentiated forms, in homogenous cultures, or as populations with other cells, and in conjunction with other bioactive ... 08/17/06 - 20060183221 - Method for neural differentiation of embryonic stem cells using protease passaging techniques The present invention provides methods for human pluripotent cell culturing and for neural cell production. More particularly, the present invention provides culturing methods employing dissociating cell cultures to an essentially single cell culture, such as by employing antibody selection and bulk passaging treatments utilizing the subsequent application of Collagenase and ... 08/10/06 - 20060177927 - Method for growth of human conjunctival tissue equivalents for research, clinical ocular surface transplantation and tissue engineering The invention relates to materials and methods for producing equivalents of tissues of the ocular surface, especially conjunctival tissue equivalents. The method of the invention involves biopsy of an appropriate tissue, primary culture in a certain medium, proliferative culture in a second medium, and differentiative culture in a third medium. ... 07/27/06 - 20060166361 - Postpartum cells derived from placental tissue, and methods of making, culturing, and using the same Cells derived from postpartum placenta and methods for their isolation are provided by the invention. The invention further provides cultures and compositions of the placenta-derived cells. The placenta-derived cells of the invention have a plethora of uses, including but not limited to research, diagnostic, and therapeutic applications. ... 07/27/06 - 20060166360 - Compositions, solutions, and methods used for transplantation This invention discloses a method for reducing the intracellular lipid storage material of a cell, tissue, or organ for transplantation and features solutions, methods and kits that induce the metabolic elimination of lipid storage in a cell, tissue, or organ. In one exemplary approach, the process involves contacting a cell, ... 07/20/06 - 20060160215 - Methods of developing a stem cell line The invention relates to a methodology for creating an embryonic stem cell line. The stem cells derived from the invention are of substantially better quality of human stem cells currently known to exist. The inventors have discovered inter alia, that when the chromosome complement of a first polar body (PB-I) ... 07/20/06 - 20060160214 - Engineered intervertebral disc tissue Surgically implantable tissue engineered intervertebral disc tissues that effectively replicate the physicochemical properties of the corresponding in vivo tissues are provided. Methods for producing such tissues can involve culturing the intervertebral disc cells to produce cells surrounded by an extracellular matrix and culturing the cells and matrix on a semipermeable ... 07/13/06 - 20060154367 - Cartilage and bone repair and regeneration using postpartum-derived cells Cells derived from postpartum tissue and methods for their isolation and induction to differentiate to cells of a chondrogenic or osteogenic phenotype are provided by the invention. The invention further provides cultures and compositions of the postpartum-derived cells and products related thereto. The postpartum-derived cells of the invention and products ... 07/13/06 - 20060154366 - Treatment of osteochondral diseases using postpartum-derived cells and products thereof Cells derived from postpartum tissue and methods for their isolation and induction to differentiate to cells of a chondrogenic or osteogenic phenotype are provided by the invention. The invention further provides cultures and compositions of the postpartum-derived cells and products such as lysates related thereto. The postpartum-derived cells of the ... 07/13/06 - 20060154365 - Cultured three dimensional tissues and uses thereof The present disclosure provides compositions of three dimensional tissue that can be administered into tissues and organs using minimally invasive methods. The three dimensional tissues elaborate a repertoire of growth factors that facilitate repair or regeneration of damaged tissues and organs. ... 07/06/06 - 20060148081 - Expansion of definitive endoderm cells Disclosed herein are cell cultures comprising expanded definitive endoderm cells as well as methods for expanding definitive endoderm cells in culture. ... 07/06/06 - 20060148080 - Methods for supporting and producing human cells and tissues in non-human mammal hosts Provided are methods for culturing and producing human cell containing compositions, such as organs and blood, in non-human mammal hosts and for the preparation of animal-hosted human cell containing compositions for transplantation to human subjects. In one aspect, non-human hosts genetically modified or treated to reduce the expression of xenoantigens, ... 07/06/06 - 20060148079 - Isolation and purification of human insulin producing cells for the treatment of insulin dependent diabetes A method for producing an ex vivo population of human insulin-producing cells that produce insulin in response to glucose from a patient is disclosed. The population of insulin producing cells is derived from human epithelial cells by causing those epithelial cells to differentiate into insulin producing cells. In one embodiment, ... 07/06/06 - 20060148078 - Method of dynamically culturing embryonic stem cells he present invention is of a method of dynamically generating human embryoid bodies which can be used for generating lineage specific cells and cell lines. Specifically, the present invention can be used to generate ESC-differentiated cells for cell-replacement therapy. ... 06/29/06 - 20060141620 - System and method for forming a cardiac tissue construct A system and method for forming a cardiac tissue construct includes a chamber and cardiac myocytes provided within the chamber. The chamber is arranged to at least partially surround an intact blood vessel in vivo to facilitate formation of the three-dimensional cardiac tissue construct within the chamber. ... 06/22/06 - 20060134782 - Differentiated cells suitable for human therapy This invention provides a system for producing differentiated cells from a stem cell population for use wherever a relatively homogenous cell population is desirable. The cells contain an effector gene under control of a transcriptional control element (such as the TERT promoter) that causes the gene to be expressed in ... 06/22/06 - 20060134781 - Three-dimensional cell culture system A three-dimensional culture system provides an efficient mechanism for producing stems cells derived from adipose tissue. A three-dimensional matrix is formed in the presence of adipose tissue to incorporate the adipose tissue into the three-dimensional matrix. After incubation, the 3-D matrix may be degraded to liberate stem cells, or progeny ... 06/22/06 - 20060134780 - Transgenic animals for producing specific isotypes of human antibodies via non-cognate switch regions The present invention provides fully human antibodies in a transgenic animal of a desired isotype in response to immunization with any virtually any desired antigen. The human immunoglobulin heavy chain transgene in the foregoing animals comprises a human constant region gene segment comprising exons encoding the desired heavy chain isotype, ... 06/15/06 - 20060128013 - Directed differentiation of embryonic cells Methods are described for mapping a pathway of differentiation of a population of embryonic cells that includes exposing the cells to an exogenous factor and measuring gene expression products that are characteristic of a particular cell type or lineage. Directing differentiation of human embryonic cells relies on dissociated embryoid bodies ... 06/15/06 - 20060128012 - Substrate recognition by differentiable human mesenchymal stem cells The invention described herein provides a structure for growing isolated differentiable human mesenchymal cells, which includes a three-dimensional matrix of fibers. The matrix serves as an implantable scaffolding for delivery of differentiable human mesenchymal cells in tissue engineering. The invention further provides compositions that contain the three-dimensional matrix of fibers ... 04/20/06 - 20060084168 - Medium and culture of embryonic stem cells Previous methods for culturing human embryonic stem cells have required either fibroblast feeder cells or a medium which has been exposed to fibroblast feeder cells in order to maintain the stem cells in an undifferentiated state. It has now been found that if high levels of fibroblast growth factor, gamma ... 04/13/06 - 20060078993 - Isolation, cultivation and uses of stem/progenitor cells The present invention relates to a method for isolating stem/progenitor cells from the amniotic membrane of umbilical cord, wherein the method comprises separating the amniotic membrane from the other components of the umbilical cord in vitro, culturing the amniotic membrane tissue under conditions allowing cell proliferation, and isolating the stem/progenitor ... 04/06/06 - 20060073588 - Method for chondrocyte expansion with phenotype retention The present invention provides a method that maintains chondrocyte phenotype during serial expansion by culturing a population of chondrocytes in a defined serum-free culture medium containing cytokines and on a substrate that is modified by covalent attachment of hyaluronic acid. The underlying principle is to maintain native chondrocyte phenotype by ... 03/23/06 - 20060063255 - Method for making dendritic cell vaccines from embryonic stem cells This disclosure provides a technology for making a dendritic cell vaccine suitable for high volume manufacturing and distribution. Human stem cells are differentiated in a multi-step protocol to generate cell populations bearing a dendritic cell phenotype. The cells are loaded by pulsing with a specific tumor antigen, or by activation ... 03/16/06 - 20060057720 - Mesenchymal cells derived from human embryonic stem cells This invention provides populations of mesenchymal cells obtained from pluripotent stem cells by differentiating them ex vivo. Multipotent mesenchymal cells can in turn be differentiated into more specialized cell types such as osteoblasts, with properties that make them suitable for reconstituting musculoskeletal cell function in an individual. The compositions, methods, ... 03/16/06 - 20060057719 - Carbohydrate determinant selection system for homologous recombination This invention provides a system for selecting a cell that has undergone genetic alteration by homologous recombination from amongst a population of cells that do not have the alteration. The successfully targeted cells are identified and separated according to surface glycosylation that has changed as a result of the homologous ... 03/16/06 - 20060057718 - Stem cell and progenitor cell expansion The present invention provides compositions and methods for the expansion of stem cells and progenitor cells with adiponectin, adiponectin variants, or other molecules that activate adiponectin receptors and signaling, whereby the stem or progenitor cells retain their pluripotential phenotype after expansion. In certain embodiments, the present invention provides adiponectin-expanded stem ... 03/16/06 - 20060057717 - Human bone accessory cells Disclosed are compositions of bone accessory cells and methods for their preparation and use. Bone accessory cells are cells which are not hematopoietic and which can reconstitute the expression of bone proteins by human bone cells and support ex vivo expansion and/or differentiation of these cells. Such bone marrow-derived accessory ... 03/09/06 - 20060051865 - Systems and methods for isolating stromal cells from adipose tissue and uses thereof Methods of isolating cells from adipose tissue that have potential to differentiate into cells of mesenchymal origin, including cells of chondrogenic, osteogenic, adipogenic, and/or myogenic origin, comprising: (a) subjecting adipose tissue to an electromagnetic, sonic, or other wave energy source; and (b) centrifuging the tissue to form a pellet comprising ... 03/09/06 - 20060051864 - Tissue culture medium for culturing potentially regenerative cells and functional tissue-organs in vitro Compositions and methods are provided for culturing in vitro potentially regenerative cells (PRCs) from which functional tissue-organs are regenerated. In one aspect of the invention, a tissue culture medium is provided which comprises at least 50% of water and a sterol compound that is dissolved in a fatty acid-containing oil ... 03/09/06 - 20060051863 - Meiosis arrest in human oocytes in vitro The method for in vitro synchronisation of nuclear and cytoplasmatic maturation of GV oocytes from domestic animals or from primates can be improved if a phosphodiesterase type 3 inhibitor is added to the medium after collection of the oocytes and, thereafter, said phosphodiesterase type 3 inhibitor is removed to allow ... 03/09/06 - 20060051862 - Methods of preparing feeder cells-free, xeno-free human embryonic stem cells and stem cell cultures prepared using same The present invention is of methods of establishing and propagating human embryonic stem cell lines using feeder cells-free, xeno-free culture systems and stem cells which are capable of being maintained in an undifferentiated, pluripotent and proliferative state in culture which is free of xeno contaminants and feeder cells. ... 02/23/06 - 20060040385 - Cultured human pancreatic islets, and uses thereof A method of generating cells capable of secreting insulin is disclosed. The method comprises subjecting mammalian embryonic stem cells to set of culturing conditions suitable for differentiation of at least a portion thereof into cells displaying at least one characteristic associated with a pancreatic islet cell progenitor phenotype, and subjecting ... 02/16/06 - 20060035373 - Stem cell libraries A stem cell library is created by genetically modifying stem cells with nucleic acids encoding polypeptides which can promote stem cell differentiation into specific cell types. Alternatively, the stem cell library is exposed to an externally added factor that promotes stem cell differentiation into a desired cell line, e.g., neuronal ... 02/09/06 - 20060030042 - Maintenance of embryonic stem cells by the gsk-3 inhibitor 6-bromoindirubin-3'-oxime The present invention relates to methods for maintaining the undifferentiated state of embryonic stem cells without the use of a feeder layer by activating the Wnt signal transduction pathway or by inhibiting glycogen synthase kinase-3 activity by contacting the cell with, inter alia, 6-bromoindirubin-3′-oxime. The present invention also relates to ... 02/02/06 - 20060024824 - Magnetic cell separation method An improved magnetic target separation method is described. In particular, the magnetic target separation method can be used to separate target entities from non-target entities in a sample contained in a vessel without needing to remove the vessel from the magnetic field gradient. ... 01/19/06 - 20060014279 - Feeder independent extended culture of embryonic stem cells Previous methods for culturing human embryonic stem cells have required either fibroblast feeder cells or a medium which has been exposed to fibroblast feeder cells in order to maintain the stem cells in an undifferentiated state. It has now been found that if an antagonist of bone morphogenic protein is ... 01/19/06 - 20060014278 - Method for programmed differentiation of stem cells Provided is a method for programming the differentiation of stem cells and for the identification of the signals responsible for cell lineage establishment of differentiated cells. ... 01/05/06 - 20060003446 - Mesoderm and definitive endoderm cell populations The present invention provides cell populations that are enriched for mesendoderm and mesoderm, and cell populations that are enriched for endoderm. The cell populations of the invention are useful for generating cells for cell replacement therapy. ... 12/29/05 - 20050287664 - Cellular proliferation control factors We discovered that Shen-Dan (SD), and its ligand HEDJ/ERdj3, are important for embryonic stem cell proliferation. These factors also promote neoplastic cell proliferation. Accordingly, compositions comprising SD or HEDJ/ERdj3 are provided, as well as uses thereof. ... 12/22/05 - 20050282275 - Adipose-derived stem cells and lattices The present invention provides adipose-derived stem cells and lattices. In one aspect, the present invention provides a lipo-derived stem cell substantially free of adipocytes and red blood cells and clonal populations of connective tissue stem cells. The invention also provides a method of isolating stem cells from adipose tissues. The ... 12/22/05 - 20050282274 - Osteoblast precursors from human embryonic stem cells This invention provides populations of mesenchymal cells obtained from pluripotent stem cells by differentiating them ex vivo. Multipotent mesenchymal cells can in turn be differentiated into more specialized cell types such as osteoblasts, with properties that make them suitable for reconstituting musculoskeletal cell function in an individual. The compositions, methods, ... 12/22/05 - 20050282273 - Medium and methods for culturing of avian primordial germ cells The invention provides a novel culture medium useful for the proliferation and maintenance of avian primordial germ cells (PGCs) and encompassing a medium base, leukemia inhibitory factor, basic fibroblast growth factor, stem cell factor and insulin-like growth factor, and an avian serum. The invention also provides a method for the ... 12/22/05 - 20050282272 - Using undifferentiated embryonic stem cells to control the immune system This disclosure provides a system for minimizing the alloreactivity of tissue transplants. The patient is administered with undifferentiated embryonic stem cells or early progenitor cells. This induces a state of inflammatory quiescence or immune unresponsiveness, which in turn enhances engraftment of cells derived from the same stem cell line given ... 12/15/05 - 20050277190 - Pancreatic stem cells Pancreatic progenitor cells isolated from the pancreas of a mammal. The invention also includes pancreatic cells or neural cells differentiated from the pancreatic progenitor cells. ... 12/08/05 - 20050272148 - Method of collecting placental stem cells A method of collecting embryonic-like stem cells from a placenta which has been treated to remove residual cord blood by perfusing the drained placenta with an anticoagulant solution to flush out residual cells, collecting the residual cells and perfusion liquid from the drained placenta, and separating the embryonic-like cells from ... 12/08/05 - 20050272147 - Methods for ex vivo propagation of somatic hair follicle stem cells The present invention is directed to methods for readily propagating somatic hair follicle stem cells or melanocyte stem cells. The methods comprise enhancing guanine nucleotide (GNP) biosynthesis, thereby expanding guanine nucleotide pools. This in turn conditionally suppresses asymmetric cell kinetics in the explanted cells. The methods of the invention include ... 12/01/05 - 20050266555 - Progenitor cells, methods and uses related thereto The present invention relates to a substantially pure population of viable pancreatic progenitor cells, and methods for isolating such cells. The present invention further concerns certain therapeutic uses for such progenitor cells, and their progeny. ... 12/01/05 - 20050266554 - Pdx1 expressing endoderm Disclosed herein are cell cultures comprising PDX1-positive endoderm cells and methods of producing the same. Also disclosed herein are cell populations comprising substantially purified PDX1-positive endoderm cells as well as methods for enriching, isolating and purifying PDX1-positive endoderm cells from other cell types. Methods of identifying differentiation factors capable of ... 12/01/05 - 20050266553 - Methods of regulating differentiation in stem cells The present invention provides methods, media and compositions capable of modulating the differentiation of stem cells. Applicants have discovered that agonists of lysophospholipid receptors and ligands of class III tyrosine kinase receptors are useful in preventing the spontaneous differentiation of stem cells. The ligands and agonists may be used alone, ... 11/24/05 - 20050260750 - Process for obtaining mammalian insulin secreting cells in vitro and their uses The present invention relates to a process for obtaining mammalian insulin secreting cells in vitro, characterized in that it contains the following steps: a) preparation of the mammalian pancreatic tissues by removal of a pancreas, b) dissociation of the pancreatic tissues obtained in step (a) into isolated pancreatic cells, c) ... 11/24/05 - 20050260749 - Differentiation of stem cells to endoderm and pancreatic lineage Methods are described to increase the proportion of endoderm committed cells and pancreatic lineage cells in a culture of human embryonic stem cells which are undergoing differentiation. The method also results in a stem cell derived cell culture which does not have tumorigenic capability. ... 11/24/05 - 20050260748 - Adult stem cells and uses thereof Disclosed are compositions and methods for isolating, immortalizing and differentiating adult stem cells, for example, particular human clonal adult liver stem cells or adipose stem cells, including specialized cell culture media for the isolation and propagation of such stem cells. Also disclosed are methods of screening for toxicity, carcinogenicity and ... 11/24/05 - 20050260747 - Embryonic stem cells and neural progenitor cells derived therefrom The present invention provides undifferentiated human embryonic stem cells, methods of cultivation and propagation and production of differentiated cells. In particular it relates to the production of human ES cells capable of yielding somatic differentiated cells in vitro, and committed progenitor cells such as neural progenitor cells capable of giving ... 11/17/05 - 20050255588 - Pluripotent embryonic-like stem cells, compositions, methods and uses thereof The present invention relates to pluripotent stem cells, particularly to pluripotent embryonic-like stem cells. The invention further relates to methods of purifying pluripotent embryonic-like stem cells and to compositions, cultures and clones thereof. The present invention also relates to a method of transplanting the pluripotent stem cells of the present ... 11/10/05 - 20050250203 - Embryonic or stem-like cell lines produced by cross species nuclear transplantation An improved method of nuclear transfer involving the transplantation of donor cell nuclei into enucleated oocytes of a species different from the donor cell is provided. The resultant nuclear transfer units are useful for the production of isogenic embryonic stem cells, in particular human isogenic embryonic or stem cells. These ... 11/10/05 - 20050250202 - Adipose stromal stem cells for tissue and vascular modification The present invention provides isolated adipose derived stromal cells and methods of use thereof. ... 11/03/05 - 20050244963 - Method for obtaining mesenchymal stem cells The invention relates to the field of cell biology. In detail it relates to the obtainment of mesenchymal stem cells from human tissue. This invention might be applicable in healing within the frame of the treatment of several diseases. Due to the invention it will be possible to obtain mesenchymal ... 11/03/05 - 20050244962 - Cultivation of primate embryonic stem cells The invention relates to methods for culturing human embryonic stem cells by culturing the stem cells in an environment essentially free of mammalian fetal serum and in a stem cell culture medium including amino acids, vitamins, salts, minerals, transferring, insulin, albumin, and a fibroblast growth factor that is supplied from ... 11/03/05 - 20050244961 - Cell culture surface The invention relates to a method for culturing mammalian cells which reduces transfer of xenobiotic material to said cells. ... 11/03/05 - 20050244960 - Preparation of type ii pneumocytes from stem cells Stem cells are induced in culture to form cells which express surfactant protein C (SPC), thereby offering a convenient and abundant supply of alveolar tissue without requiring lung donation or the use of cadavers. Stem cells are cultured to give embryoid bodies, which are they maintained under conditions which cause ... 10/20/05 - 20050233448 - Materials and methods to produce stem cells A method of generating stem cells, such as human and mouse embryonic stem cells, from a stem cell culture comprising the step of perfusing the stem cell culture by flowing culture media through the stem cell culture and apparatus for generating stem cells are disclosed. Also disclosed are methods and ... 10/20/05 - 20050233447 - Dedifferentiated, programmable stem cells of monocytic orgin, and their production and use The invention relates to the production of adult dedifferentiated, programmable stem cells from human monocytes by cultivation of monocytes in a culture medium which contains M-CSF and IL-3. The invention further relates to pharmaceutical preparations, which contain the dedifferentiated, programmable stem cells and the use of these stem cells for ... 10/20/05 - 20050233446 - Defined media for stem cell culture Stem cells, including mammalian, and particularly primate primordial stem cells (pPSCs) such as human embryonic stem cells (hESCs), hold great promise for restoring cell, tissue, and organ function. However, cultivation of stem cells, particularly undifferentiated hESCs, in serum-free, feeder-free, and conditioned-medium-free conditions remains crucial for large-scale, uniform production of pluripotent ... 10/20/05 - 20050233445 - Biomimetic urothelium Urothelium is the specialised endothelium that lines much of the mammalian urinary tract. This invention relates to the field of in vitro urothelial culture. Previous methods produce tissue lacking much of the functionality of native urothelium. In this invention, stratified, differentiated mammalian urothelium is produced by passaging urothelial cells through ... 10/13/05 - 20050227353 - Methods of inducing differentiation of stem cells The present invention relates to methods of inducing differentiation of stem cells. In particular, the invention relates to methods of inducing differentiation of embryonic stem cells into muscle cells or vascular endothelial cells. The invention also includes cells, cell lines, testing models and culture systems used in the methods of ... 10/06/05 - 20050221478 - Control of es cell self renewal and lineage specification, and medium therefor Self renewal of pluripotent cells in culture is promoted using a combination of an activator of a signalling pathway downstream of a receptor of the TGF-β superfamily and an activator of a gp130 downstream signalling pathway. ... 10/06/05 - 20050221477 - Protein sustaining undifferentiated stem cells as such The sustaining stem cells for example, hematopoietic stem cells while holding in the undifferentiated state and maintaining pluripotency by providing a polypeptide having a WIF domain for example, WIF-1 to the stem cells has been successfully achieved. This polypeptide preferably also contains an EGF-like repeat. This result has been achieved ... 09/29/05 - 20050214939 - Direct differentiation method for making cardiomyocytes from human emberyonic stem cells This invention provides a new procedure for generating cardiomyocyte lineage cells from embryonic stem cells for use in regenerative medicine. Differentiating by way of embryoid body formation or in serum is no longer required. Instead, the stem cells are plated onto a solid substrate, and differentiated in the presence of ... 09/29/05 - 20050214938 - Cardiac bodies: clusters of spontaneously contracting cells for regenerating cardiac function This disclosure describes clusters of cardiomyocyte lineage cells referred to as cardiac bodies. They can be obtained by differentiating human embryonic stem cells into cells that express cardiomyocyte markers, and separating cells according to their density. Single suspended cells are removed, leaving self-aggregating clusters that can be propagated and enriched ... 09/22/05 - 20050208653 - Method of isolating bile duct progenitor cells The present invention relates to a substantially pure population of viable bile duct progenitor cells, and methods for isolating such cells. The present invention further concerns certain therapeutic uses for such progenitor cells, and their progeny. ... 09/22/05 - 20050208652 - Isogenic beta-catenin cell lines, and methods of making and using same A set of isogenic cell lines, which includes a first population of cells that express only a wild type β-catenin polypeptide and at least a second population of cells that express only an activated β-catenin polypeptide, is provided. A set of isogenic cells, including a first population of cells that ... 09/22/05 - 20050208651 - Vitro maturation of immature human oocytes The invention provides methods for in vitro maturation of immature human oocytes. In one aspect, the invention provides a method for in vitro maturation of immature human oocytes, comprising the steps of: (a) inducing in a female human subject an increase in endogenous luteinizing hormone levels, said subject having not ... 09/08/05 - 20050196859 - Propagation and/or derivation of embryonic stem cells Embryonic stem (ES) cells are cultured in the presence of a compound which selectively inhibits propagation or survival of cells other than ES cells. The ES cells have not been genetically altered. Instead, the compound inhibits a signalling pathway which is essential for propagation of differentiated cells but is not ... 09/08/05 - 20050196858 - Isolation, selection and propagation of animal transgenic stem cells Animal stem cells are obtained and maintained by culturing cells containing, in the genome, a selectable marker. Differential expression of the selectable marker enables preferential survival and/or division of the desired stem cells compared to the non-stem cells. ... 09/01/05 - 20050191744 - Method for differentiating primate embryonic stem cell into vascular cell To provide a technique of the differentiation from a primate embryonic stem cell into a vascular cell, and techniques using the same. A method for differentiating a primate embryonic stem cell into a vascular cell, comprising differentiating a primate embryonic stem cell into a VEGFR-2-positive and undifferentiated primate embryonic stem ... 09/01/05 - 20050191743 - Three-dimensional peripheral lymphoid organ cell cultures The present invention relates to a method of culturing peripheral lymphoid organ cells on a three-dimensional scaffolding which is covered or surrounded with culture medium, under conditions effective to generate and maintain mature and functional lymphoid cells, where the three-dimensional scaffolding allows cells in the culture medium to have cell ... 09/01/05 - 20050191742 - Trophoblast cell preparations Stable pluripotent trophoblast stem (TS) cell lines and uses of the cell lines are described. The cell lines comprise cells that (i) are capable of indefinite proliferation in vitro in an undifferentiated state; and (ii) are capable of differentiation into cells of the trophoblast lineage in vivo. ... 08/11/05 - 20050176140 - Method and apparatus for cell culture using a two liquid phase bioreactor Advanced Bioreactor Cell Culture Technology presents a method of cell culturing and bioprocessing incorporating molecular biology techniques, advanced process control methodology, and a process control interface applied to a two liquid phase cell culture bioreactors to proliferate, grow, and expand non-differentiated precursor cells, embryonic stem (ES) cells, endocrine progenitor cells, ... 08/04/05 - 20050170501 - Method for preparing tissue constructs The present invention relates to a method of assembling a single living tissue sheet formed of different cell populations. According to this invention at least two cell populations composing at least two living tissue sheets can be assembled into a single continuous tissue construct. The cells of this construct can ... 07/28/05 - 20050164383 - Embryonic stem cells The present invention relates to undifferentiated human embryonic stem cells, methods of cultivation and propagation, production of differentiated cells and in particular the production of human embryonic stem cells capable of yielding somatic differentiated cells in vitro, as well as committed progenitor cells capable of giving rise to mature somatic ... 07/28/05 - 20050164382 - Differentiation protocol for making human cardiomyocytes This invention provides populations human cells of the cardiomyocyte lineage. The cells are obtained by causing cultures of pluripotent stem cells to differentiate in vitro, and then harvesting cells with certain phenotypic features. Differentiated cells bear cell surface and morphologic markers characteristic of cardiomyocytes, and a proportion of them undergo ... 07/28/05 - 20050164381 - Primate embryonic stem cells A purified preparation of primate embryonic stem cells is disclosed. This preparation is characterized by the following cell surface markers: SSEA-1 (−); SSEA-4 (+); TRA-1-60 (+); TRA-1-81 (+); and alkaline phosphatase (+). In a particularly advantageous embodiment, the cells of the preparation are human embryonic stem cells, have normal karyotypes, ... 07/28/05 - 20050164380 - Stem cell culture medium and method of using said medium and the cells The present invention relates to methods and compositions concerning isolation of proliferating cells. In particular, the invention regards enrichment of stem cells in a mixture of stem cells and non-stem cells, wherein the non-stem cells may be differentiated cells. The invention exploits the non-adherent property of stem cells, as opposed ... 07/28/05 - 20050164379 - Stable cell lines expressing herg1a and herg1b A line of cultured mammalian cells includes HERG1b subunits and optionally HERG1a subunits. ... 07/28/05 - 20050164378 - Method for the production of a nerve transplant In a procedure to produce a nerve or other transplantation material, the basal membranes of muscles are colonized with Schwann cells. The colonized basal membrane structure is placed into a nutrient Solution. The Schwann cells are positioned generally continuously along the longitudinal direction of the fibers of the basal membrane ... 07/28/05 - 20050164377 - Base material for culturing embryo stem cells and culture method According to the present invention, capable of safely holding a large amount of undifferentiated embryonic stem cells to culture in the absence of feeder cells or feeder cell-derived components. Cultured embryonic stem cells can be applied to the fields of cell culture, tissue transplantation, drug development, and gene therapy. ... 07/21/05 - 20050158854 - Primate embryonic stem cells A purified preparation of primate embryonic stem cells is disclosed. This preparation is characterized by the following cell surface markers: SSEA-1 (−); SSEA-4 (+); TRA-1-60 (+); TRA-1-81 (+); and alkaline phosphatase (+). In a particularly advantageous embodiment, the cells of the preparation are human embryonic stem cells, have normal karyotypes, ... 07/21/05 - 20050158853 - Definitive endoderm Disclosed herein are cell cultures comprising definitive endoderm cells and methods of producing the same. Also disclosed herein are cell populations comprising substantially purified definitive endoderm cells as well as methods for enriching, isolating and purifying definitive endoderm cells from other cell types. ... 07/21/05 - 20050158852 - Methods for culturing human embryonic stem cells The present invention discloses a method for improving growth and survival of single human embryonic stem cells. The method includes the step of obtaining a single undifferentiated HES cell; mixing the single undifferentiated cell with an extracellular matrix (ECM) to encompass the cell; and inoculating the mixture onto feeder cells ... 07/14/05 - 20050153445 - System for expanding and differentiating human embryonic stem cells This disclosure provides an improved system for culturing human pluripotent stem cells. Traditionally, pluripotent stem cells are cultured on a layer of feeder cells (such as mouse embryonic fibroblasts) to prevent them from differentiating. In the system described here, the role of feeder cells is replaced by components added to ... 07/14/05 - 20050153444 - Using fibroblast growth factor to establish a line of embryonic stem cells This disclosure provides an improved system for culturing human pluripotent stem cells. Traditionally, pluripotent stem cells are cultured on a layer of feeder cells (such as mouse embryonic fibroblasts) to prevent them from differentiating. In the system described here, the role of feeder cells is replaced by components added to ... 07/14/05 - 20050153443 - Method for facilitating the production of differentiated cell types and tissues from embryonic and adult pluripotent and multipotent cells The invention is concerned with producing differentiated cells, tissues and organs from pluripotent and mutlipotent cells. The methods of the invention are particularly useful for producing differentiated cells from pluripotent cells wherein communication between the cells of more than one embryonic germ layer or more than one organ system are ... 07/14/05 - 20050153442 - Adipose-derived stem cells and lattices The present invention provides adipose-derived stem cells (ADSCs), adipose-derived stem cell-enriched fractions (ADSC-EF) and adipose-derived lattices, alone and combined with the ADSCs of the invention. In one aspect, the present invention provides an ADSC substantially free of adipocytes and red blood cells and clonal populations of connective tissue stem cells. ... 07/14/05 - 20050153441 - Adipose-derived stem cells and lattices The present invention provides adipose-derived stem cells (ADSCs), adipose-derived stem cell-enriched fractions (ADSC-EF) and adipose-derived lattices, alone and combined with the ADSCs of the invention. In one aspect, the present invention provides an ADSC substantially free of adipocytes and red blood cells and clonal populations of connective tissue stem cells. ... 07/07/05 - 20050148070 - Cultivation of primate embryonic stem cells The invention relates to methods for culturing human embryonic stem cells by culturing the stem cells in an environment essentially free of mammalian fetal serum and in a stem cell culture medium including amino acids, vitamins, salts, minerals, transferring, insulin, albumin, and a fibroblast growth factor that is supplied from ... 07/07/05 - 20050148069 - Igf-1 instructs multipotent adult cns neural stem cells to an oligodendroglial lineage Adult neural stem cells differentiate into neurons, astrocytes, and oligodendrocytes in the mammalian CNS, but the molecular mechanisms that control their differentiation are not yet well understood. Insulin-like growth factor-I (IGF-I) can promote the differentiation of cells already committed to an oligodendroglial lineage during development. However, it is unclear whether ... 06/30/05 - 20050142659 - Purified populations of stem cells The invention is directed to a purified population of mammalian endothelial, muscle, or neural stem cells. The invention further provides methods for isolating such populations of cells; methods for using such populations of cells for treating mammals; methods for making vectors for gene therapy; and methods for carrying out gene ... 06/23/05 - 20050136536 - Embryonic epithelial cells A population of embryonic epithelial cells produced in vitro from embryonic stem cells. In one embodiment, at least 45% of the cells express cytokeratin, for example, cytokeratin-7. ... 06/16/05 - 20050130299 - Closed cell culture system An object of the present invention is to provide a tissue cell culture system whereby a call tissue can be efficiently and quickly proliferated in vivo and the onset bacterial infection in an injured part can be avoided in the course of a treatment. More specifically, a closed cell culture ... 06/09/05 - 20050124063 - Culture system and method for maintenance and proliferation of undifferentiated human embryonic stem cells The present invention discloses an improved culture system for proliferative and undifferentiated growth of human embryonic stem cells, comprising an extracellular matrix prepared from feeder cells and a conditioned medium preconditioned by feeder cells. This invention also relates to a culture method utilizing the foregoing culture system. ... 06/09/05 - 20050124062 - Milk protein biofilm and uses thereof A biofilm for preparing laminated tissue constructs comprising at least one milk protein forming a sheet like structure can be used to support at least one layer of cells adhered to that. The biofilm allows for a easy handling of cultured cell sheets that can be used as tissue graft. ... 06/02/05 - 20050118713 - Morula derived embryonic stem cells A method for isolating a human pluripotent embryonic stem cell line derived from culturing morula stage human embryo cells is disclosed. The method includes culturing the cells in close contact with a feeder cell layer to inhibit differentiation of the cells. A preparation of human pluripotent embryonic stem cells derived ... 06/02/05 - 20050118712 - Two-stage culture protocol for isolating mesenchymal stem cells from amniotic fluid A method of isolating mesenchymal stem cells from amniotic fluid using a two-stage culture protocol comprising culturing amniocytes and culturing mesenchymal stem cells. For culturing amniocytes, primary cultures are set up using routine or standard culture protocol in a cytogenetic laboratory. Non-adhering amniotic fluids cells in the supernatant medium are ... 06/02/05 - 20050118711 - Device for applying liquid media and corresponding method The invention relates to a device (31) for applying liquid media, particularly culture media and/or reaction media, and a method fur generating suitable reaction and/or cultivation conditions. According to one preferred embodiment, said device stands on four legs (32). The drops (33) can be seen on the bottom side of ... ### FreshPatents.com Support |