REGISTER now for FREE 
|
FREE patent keyword monitoring and additional FREE benefits. REGISTER now for FREE
|
|
|
Chemistry: Molecular Biology And Microbiology > Micro-organism, Tissue Cell Culture Or Enzyme Using Process To Synthesize A Desired Chemical Compound Or Composition > Enzymatic Production Of A Protein Or Polypeptide (e.g., Enzymatic Hydrolysis, Etc.) Enzymatic Production Of A Protein Or Polypeptide (e.g., Enzymatic Hydrolysis, Etc.)Enzymatic Production Of A Protein Or Polypeptide (e.g., Enzymatic Hydrolysis, Etc.) patent applications listed are from June 2005 to current and include Date, Patent Application Number, Patent Title, Patent Abstract summary and are linked to the corresponding patent application page.04/19/07 - 20070087404 - Poly-beta-peptides from functionalized beta-lactam monomers and antibacterial compositions containing same Disclosed is a method of making β-polypeptides. The method includes polymerizing β-lactam-containing monomers in the presence of a base initiator and a co-initiator which is not a metal-containing molecule to yield the product β-polypeptides. Specifically disclosed are methods wherein the base initiator is potassium t-butoxide, lithium bis(trimethylsilyl)amide (LiN(TMS)2), potassium bis(trimethyl-silyl)amide, ... 04/12/07 - 20070082378 - Convergent synthesis of proteins by kinetically controlled ligation The present invention concerns methods and compositions for synthesizing a polypeptide using kinetically controlled reactions involving fragments of the polypeptide for a fully convergent process. In more specific embodiments, a ligation involves reacting a first peptide having a protected cysteyl group at its N-terminal and a phenylthioester at its C-terminal ... 04/05/07 - 20070077618 - Efficient production method of ascopyrone p A method for producing ascopyrone P from 1,5-D-anhydrofructose efficiently is provided. The method produces ascopyrone P by heating a solution of 1,5-D-anhydrofructose at a pH of 10 or less and a temperature of 100° C. or higher. ... 04/05/07 - 20070077617 - Polypeptide cleavage method using ompt protease variant A polypeptide cleavage method characterized in that arginine or lysine is at the P1 position of a desired cleavage site in a polypeptide, an amino acid other than aspartic acid, glutamic acid or proline is at the P1′ position, a single basic amino acid or two or three consecutive basic ... 03/08/07 - 20070054352 - Hydrolysed casein product comprising tripeptides ipp and/or vpp Process for the preparation of a hydrolysed casein product comprising tripeptides VPP, IPP and/or LPP, wherein a substrate comprising casein or casein fragments is subjected to enzyme treatment wherein the enzyme is derived from Aspergillus, wherein the enzyme concentration is 2-10 wt. % based on casein and that the enzyme ... 03/01/07 - 20070048817 - Polynucleotides and polypeptides coded by said polynucleotides involved in the synthesis of diketopiperazine derivatives The invention concerns novel isolated natural or synthetic polynucleotides and polypeptides coded by said polynucleotides, involved in the synthesis of diketopiperazine derivatives, vectors comprising said polynucleotides, micro-organisms transformed with said polynucleotides, uses of said polynucleotides and said polypeptides, as well as methods for the synthesis of diketopiperazine derivatives, including cyclodipeptides ... 02/22/07 - 20070042459 - Dipeptide production method, l-amino acid amide hydrolase used therein, and production method of l-amino acid amide hydrolase A process for industrially advantageously producing a dipeptide via a convenient pathway starting with less expensive and easily available materials is provided. A dipeptide is produced from an L-amino acid amide and an L-amino acid by using a culture of a microbe capable of synthesizing the dipeptide from the L-amino ... 02/22/07 - 20070042458 - Erythropoietin: remodeling and glycoconjugation of erythropoietin The invention includes methods and compositions for remodeling a peptide molecule, including the addition or deletion of one or more glycosyl groups to a peptide, and/or the addition of a modifying group to a peptide. ... 02/15/07 - 20070037245 - Method of posttranslational modification by adding mycrosomal membrane in cell-free protein synthesis It is intended to provide a novel method for posttranslational modification in cell-free protein synthesis and a novel method for cell-free protein synthesis with the use of such posttranslational modification reaction. A method for synthesizing a protein in a cell-free system using an extract liquid for cell-free protein synthesis, characterized ... 02/01/07 - 20070026485 - Glycopegylation methods and proteins/peptides produced by the methods The invention includes methods and compositions for remodeling a peptide molecule, including the addition or deletion of one or more glycosyl groups to a peptide, and/or the addition of a modifying group to a peptide. ... 01/04/07 - 20070004001 - Total amino acid stabilization during cell-free protein synthesis Compositions and methods are provided for the enhanced in vitro synthesis of protein molecules, by optimizing the metabolism of amino acids present in the reaction mix, preferably all amino acids in the reaction mixture. By performing synthesis with extracts from genetically modified microbial strains that are deficient in multiple amino ... 12/28/06 - 20060292667 - Method for improving solubility and folding efficiency of target proteins using rna as molecular chaperone Disclosed is a method for improving folding efficiency and solubility of a target protein linked to a RNA-binding protein by using RNA molecule as a molecular chaperone, wherein the RNA molecule interacts with the RNA-binding protein. More particularly, the present invention discloses method for improving folding efficiency and solubility of ... 12/21/06 - 20060286629 - Processing of peptides and proteins The invention provides novel methionine aminopeptidase enzymes, their use, and related compounds and methods. ... 12/14/06 - 20060281145 - Methods for introducing mannose 6-phosphate and other oligosaccharides onto glycoproteins Methods to introduce highly phosphorylated mannopyranosyl oligosaccharide derivatives containing mannose 6-phosphate (M6P), or other oligosacharides bearing other terminal hexoses, to carbonyl groups on oxidized glycans of glycoproteins while retaining their biological activity are described. The methods are useful for modifying glycoproteins, including those produced by recombinant protein expression systems, to ... 11/30/06 - 20060269987 - Process for making a low molecular weight gelatine hydrolysate and gelatine hydrolysate compositions The present invention provides a process to make a gelatine hydrolysate, a gelatine hydrolysate and gelatine compositions comprising gelatine hydrolysates. More specifically, the invention provides gelatine compositions having improved cross-linking and dissolution properties. ... 11/09/06 - 20060252115 - Synthesis and use of anti-reverse mrna cap analogues The ability to synthesize capped RNA transcripts in vitro has been of considerable value in a variety of applications. However, one-third to one-half of the caps have, until now, been incorporated in the reverse orientation. Such reverse caps impair the translation of in vitro-synthesized mRNAs. Novel cap analogues, such as ... 11/02/06 - 20060246537 - Multi-step process for the manufacture of therapeutic protein A process for the production of therapeutic protein is provided, which includes the steps of preparing nutrient medium for culturing of cells to express the protein, culturing the cells in the presence of the nutrient medium to express the protein, preparing protein separation solution for isolating the protein, formulating the ... 10/19/06 - 20060234335 - Conjugated thiophene compound, conprising perfluorinated and alkylated sidechains, conductive organic thin film containing the compound, and field-effect type organic transistor employing the thin film where R1 and R2 are hydrogen or a substituted or unsubstituted linear, cyclic, or branched alkyl group of 1 to 20 carbon atoms, at least one of R1 and R2 being not hydrogen; and R3 and R4 are hydrogen or a substituted or unsubstituted linear, cyclic, or branched perfluoroalkyl group ... 10/05/06 - 20060223140 - Transglycosylation method and protein having transglycosylation activity The present invention provides a transglycosylation method and a protein catalyzing a transglycosylation reaction. A reaction of transferring a galactosylβ1→3N-acetylgalactosaminyl group from a sugar donor containing a sugar chain having a galactosylβ1→3N-acetylgalactosaminyl group bound thereto at the α-position, to a sugar chain acceptor, is carried out in the presence of ... 09/21/06 - 20060211083 - Products and processes for in vitro synthesis of biomolecules Provided herein are products and processes for efficiently synthesizing biomolecules in vitro using cell-free extracts derived from mammalian cells and insect cells. In an embodiment, provided is a process for preparing a cell-free extract from insect cells and mammalian cells that efficiently synthesizes post-translationally modified target proteins (e.g., glycosylated target ... 09/14/06 - 20060205030 - Dna for encoding d-hydantoin hydrolases, dna for encoding n-carbamyl-d-amino acid hydrolases, recombinant dna containing the genes, cells transformed with the recombinant dna, methods for producing proteins utilizing the transformed cells and methods for DNA for encoding a protein having D-hydantoinase activity which has a base sequence represented by Sequence ID No. 1 in the Sequence Listing. DNA for encoding a protein having D-carbamylase activity which has a base sequence represented by Sequence ID No. 3 in the Sequence Listing. ... 09/07/06 - 20060199245 - Conus protein disulfide isomerase Protein disulfide isomerase is a major component of Conus venom ducts. The invention relates to a protein disulfide isomerase (PDI) from Conus snails, a nucleic acid sequence encoding the Conus protein disulfide isomerase, and to methods for folding disulfide-rich peptides using a protein disulfide isomerase. Oxidative folding of conotoxin precursors, ... 08/10/06 - 20060177893 - Method for producing dipeptides The present invention provides a method for producing a dipeptide from starting materials that are available at low costs through a route industrially advantageous and simple. Dipeptides are produced from amino acid esters and amino acids by using a culture of a microbe having an ability to produce a dipeptide ... 08/10/06 - 20060177892 - Intracellular formation of peptide conjugates The invention includes methods and compositions for forming peptide conjugates intracellularly having a covalent linkage between a modifying group and a glycosylated or non-glycosylated peptide. The modifying group is conjugated to the peptide via a glycosyl linking group interposed between and covalently linked to both the peptide and the modifying ... 06/29/06 - 20060141559 - Extract from cultured mammalian cell, process for preparation thereof and method of cell-free protein synthesis using the extract A method for preparing a cultured mammalian cell extract liquid, comprising at least the step of rapidly freezing a cultured mammalian cell suspended in a solution for extraction; a cultured mammalian cell extract liquid prepared by the process; and a method for cell-free protein synthesis using the extract liquid. Preferably, ... 06/22/06 - 20060134737 - Ubiquitinated tnf receptor2 and its uses The present invention relates to ubiquitinated TNF receptor 2, and the uses thereof. More specifically, the invention relates to the use of TNF receptor 2 ubiquitination to deplete TNF receptor 2 from the cell membrane and cytoplasm, and relocalize it in the insoluble cell fraction. Such relocalization can be used ... 06/22/06 - 20060134736 - Human growth hormone conjugated with biocompatible polymer The present invention relates to conjugates of biocompatible polymers and hGH, particularly PEG-hGH, where the activated biocompatible polymer is conjugated to a carboxyl group of hGH at a molar ratio of 2:1 or less, preferably 1:1, methods of preparation, and related pharmaceutical compositions. The PEG-hGH conjugates have up to 20% ... 06/22/06 - 20060134735 - Process for producing extract for cell-free protein synthesis and cell extract produced thereby It is intended to provide a process for producing an extract for cell-free protein synthesis whereby the productivity of a protein and the production efficiency can be improved. Cells are cultured under suppressed growth conditions. In the stationary phase of the culture, the cells are collected and then disrupted. The ... 06/08/06 - 20060121560 - Dna fragment to promote translation reaction and method for cell-free protein synthesis system using the same The present invention provides a DNA fragment allowing easy cloning of a desired gene and capable of further improving translation efficiency, a protein expression vector and a template DNA having the DNA fragment, a mRNA obtained from the template DNA, a reaction solution for cell-free protein synthesis system containing the ... 06/08/06 - 20060121559 - Saccharide composition purification instrument The present invention provides a sugar chain purification instrument which can continuously and efficiently purify the sugar chain. According to the present invention, the instrument includes an immobilized protease column in which a carrier which can be packed in the column and on which protease is immobilized is packed and ... 05/25/06 - 20060110788 - Feeding buffers, systems, and methods for in vitro synthesis of biomolecules Compositions, methods and kits for in vitro systems for synthesis of biomolecules such as polypeptides, are provided herein. Cell extracts that provide enhanced yields of soluble proteins using in vitro protein synthesis methods are provided. The invention also includes methods for producing high yields of proteins by the addition of ... 05/11/06 - 20060099672 - Activatable recombinant neurotoxins Compositions comprising activatable recombinant neurotoxins and polypeptides derived therefrom. The invention also comprises nucleic acids encoding such polypeptides, and methods of making such polypeptides and nucleic acids. ... 05/11/06 - 20060099671 - Methods and compositions for generating angiostatin The invention provides methods of generating angiostatin in vitro comprising contacting plasminogen with a plasminogen activator and a sulfhydryl donor or contacting plasmin with a sulfhydryl donor. The invention also provides a method of treating angiogenic diseases by administering to an animal suffering from such a disease a sulfhydryl donor, ... 04/27/06 - 20060088906 - Erythropoietin: remodeling and glycoconjugation of erythropoietin The invention includes methods and compositions for remodeling a peptide molecule, including the addition or deletion of one or more glycosyl groups to a peptide, and/or the addition of a modifying group to a peptide. ... 04/20/06 - 20060084137 - Use of defibrinated blood for manufacture of hemoglobin-based oxygen carrier Red blood cells are purified by defibrinating whole blood and then filtering the defibrinated whole blood, whereby at least a portion of a plasma component is separated from the red blood cells to form a suspension of red blood cells, thereby purifying the red blood cells. Whole blood is defibrinated ... 04/20/06 - 20060084136 - Production of fusion proteins by cell-free protein synthesis The present invention relates to in vitro protein synthesis (IVPS) systems, particularly such systems using suppressor tRNAs and rare codon tRNAs to extend translation of an open reading frame into fusion protein elements, thereby generating fusion proteins in vitro. The invention also provides methods, compositions and kits using the IVPS ... 04/13/06 - 20060078963 - Methods for producing modified glycoproteins Cell lines having genetically modified glycosylation pathways that allow them to carry out a sequence of enzymatic reactions, which mimic the processing of glycoproteins in humans, have been developed. Recombinant proteins expressed in these engineered hosts yield glycoproteins more similar, if not substantially identical, to their human counterparts. The lower ... 04/13/06 - 20060078962 - Polysaccharide-based polymers and methods of making the same Gels and polymers comprising a polypeptide bound to a polysaccharide are disclosed. Specific polypeptides include, but are not limited to, polypeptides that comprise glutamine or tyrosine residues. Specific polysaccharides include, but are not limited to, chitosan. Gels and polymers of the invention can be used for the in vitro and ... 04/06/06 - 20060073543 - Process for the preparation of angiotensis converting enzyme (ace) inhibitors and its use Process for the preparation of angiotensis converting enzyme(ace)inhibitors and use thereof ... 04/06/06 - 20060073542 - Recombinant glycosyltransferase fusion proteins This invention provides recombinant glycosyltransferase fusion proteins having a desired level of expression and enzymatic activity (for example, acceptor substrate specificity or catalytic activity). The fusion proteins of the invention have a functional domain of a first glycosyltransferase joined, directly or through a peptide linker, to a subsequence of a ... 03/30/06 - 20060068462 - Method A method of producing one or more of a carbohydrate ester, a protein ester, a protein subunit ester or a hydroxyl acid ester, which method comprises admixing an acyl donor, an acyl acceptor and water to produce a high water environment comprising 5-98% water, wherein said acyl donor is a ... 03/30/06 - 20060068461 - Transglycosylation method and protein having transglycosylation activity The present invention provides a transglycosylation method and a protein catalyzing a transglycosylation reaction. A reaction of transferring a galactosylβ1→3N-acetylgalactosaminyl group from a sugar donor containing a sugar chain having a galactosylβ1→3N-acetylgalactosaminyl group bound thereto at the α-position, to a sugar chain acceptor, is carried out in the presence of ... 03/09/06 - 20060051834 - Method for preparative in vitro protein biosynthesis The invention relates to a method for preparative in vitro protein synthesis of an expression product in a cell-free transcription/translation system, comprising the following steps: a) in a reaction vessel, a reaction solution is prepared, comprising the following synthesis substances: components of the transcription/translation apparatus for a defined expression product, ... 02/23/06 - 20060040345 - Deglycosylated enzymes for conjugates By deglycosylating an enzyme derived from a eukaryotic organism prior to forming a conjugate, i.e. prior to attaching the enzyme to a molecule capable of binding to a target molecule, the sensitivity of an assay for detecting the presence or determining the quantity of a target molecule, in which the ... 02/16/06 - 20060035313 - Proteolytic fermenter A process of controlling the production of enzymatic protein hydrolysate from cold water animals, containing up to 90% free amino acids, cold fish oil, hydroxilaptite (calcium orthophosphate), proteins, peptides, vitamin, minerals and calcium salts of phosphorus. The process provides a greater degree of refining than existing technology through the use ... 02/09/06 - 20060029995 - novel class of metacaspases The invention relates to a novel class of metacaspases. More particularly, the present invention relates to the use of metacaspases, preferably plant metacaspases to process a protein at a cleavage site comprising an arginine or a lysine at the P1 position, and to the use of such metacaspases to modulate ... 02/02/06 - 20060024779 - Glycosulfopeptide inhibitors of leukocyte rolling and methods of use thereof Compounds, compositions and methods for treating conditions characterized by leukocyte rolling are described. The compounds contain glycosulfopeptide structures comprising sulfated tyrosines and sialyated, fucosylated N-acetyllactosamino glycans. The glycosulfopeptides may be conjugated or complexed to other compounds for enhancing serum half-life or for controlled release, for example. Examples of conditions treated ... 02/02/06 - 20060024778 - Method for enzymatic production of glp-2(1-33) and glp-2(1-34) peptides The invention provides methods for making peptides from a polypeptide containing at least one copy of the peptide using clostripain to excise the peptide from the polypeptide. The methods enable the use of a single, highly efficient enzymatic cleavage to produce any desired peptide sequence. ... 02/02/06 - 20060024777 - Process for the preparation of high arginine peptides A process of preparation of non-bitter high arginine peptide from groundnut, which has an arginine to lysine ratio of at least 5 and a yield of 17-20%. ... 01/19/06 - 20060014240 - Extraction and purification method of active constituents from stem of lonicera japonica thunb., its usage for anti-inflammatory and analgesic drug Disclosed is a method for extracting and purifying active constituents from honeysuckle (Lonicera japonica Thunb.) and its use. More particularly, this invention relates to an extraction and purification method of active constituents including sweroside from for honeysuckle stem (stem of honeysuckle where leaves are removed) by removing tannins, hardly soluble ... 01/12/06 - 20060008871 - Extract of e. coli cells having mutation in ribosomal protein s12, and method for producing protein in cell-free system using the extract It is intended to provide an extract, kit and a process for synthesizing a protein in a cell-free system. The extract is characterized by being prepared from mutant Escherichia coli cells having a mutation in the ribosomal protein S12 gene. In a preferred embodiment, the mutation is such a mutation ... 01/12/06 - 20060008870 - Method for universal enzymatic production of bioactive peptides The invention provides methods for making peptides from a polypeptide containing at least one copy of the peptide using clostripain to excise the peptide from the polypeptide. The methods enable the use of a single, highly efficient enzymatic cleavage to produce any desired peptide sequence. ... 12/29/05 - 20050287627 - Process for producing dipeptides or dipeptide derivatives The present invention provides a process for producing a dipeptide or a dipeptide derivative using a phosphate donor, a substance selected from the group consisting of adenosine-5′-monophosphate, adenosine-5′-diphosphate and adenosine-5′-triphosphate, one or more kinds of amino acids or amino acid derivatives, and as enzyme sources, a protein having polyphosphate kinase ... 12/29/05 - 20050287626 - Process for producing dipeptides The present invention provides a process for producing a dipeptide which comprises culturing in a medium a microorganism which has the ability to produce a protein having the activity to form the dipeptide from one or more kinds of amino acids and which has the ability to produce at least ... 11/24/05 - 20050260701 - Method for enzymatic production of glp-1 (7-36) amide peptides The invention provides methods for making peptides from a polypeptide containing at least one copy of the peptide using clostripain to excise the peptide from the polypeptide. The methods enable the use of a single, highly efficient enzymatic cleavage to produce any desired peptide sequence. ... 11/17/05 - 20050255542 - Method for producing by cell-free protein synthesis system using thioredoxin-fused protein expression vector A fusion protein of thioredoxin with a target protein is expressed in a cell-free protein synthesis system to thereby synthesize at least a part of the fusion protein as a soluble protein. It is preferable that the target protein is a highly hydrophobic protein such as a membrane protein. In ... 11/10/05 - 20050250177 - Preparation, characterization and quantification of active fractions that promote cell growth and recombinant-protein expression A process for preparing an active animal cell-growth-enhancing fraction of a hydrolysate of plant tissue, animal tissue or microorganism ultrafiltrate material, especially a fraction of a yeastolate ultrafiltrate, comprising forming a precipitate from said fraction with a water-soluble solvent is disclosed. Such solvents as alkanols, alkyl sulfoxides, ketones or alkyl ... 11/03/05 - 20050244920 - Protein synthesis system using xenopus oocyte extract It is intended to provide a cell-free protein synthesis system with the use of a Xenopus oocyte extract, which is superior in protein synthesis efficiency to the microinjection method into oocytes or the existing method with the use of an egg extract. Namely, a kit for use in a cell-free ... 10/27/05 - 20050239162 - Process for folding chemically synthesized polypeptides A method is provided for folding chemically synthesized polypeptides having two or more derivatized cysteine residues by contacting with a reducing agent in a folding buffer having a predetermined pH and temperature. ... 10/20/05 - 20050233412 - Glycoprotein synthesis Methods for making glycoproteins, both in vitro and in vivo, are provided. One method involves incorporating an unnatural amino acid into a protein and attaching one or more saccharide moieties to the unnatural amino acid. Another method involves incorporating an unnatural amino acid that includes a saccharide moiety into a ... 10/20/05 - 20050233411 - Compositions and methods for cleaving iap The present invention relates to compositions and methods for making and using Omi-related and IAP-cleaving nucleotide sequences, mutant nucleotide sequences, and polypeptide sequences expressed therefrom, including both biologically active and inactive molecules. The present invention relates to cleaving IAP using an Omi polypeptide. ... 10/13/05 - 20050227314 - Polypeptide cleavage process The invention provides a palladium-promoted hydrolytic polypeptide cleavage process which selectively cleaves the polypeptide at a Cys-His cleavage site comprising solubilizing the polypeptide in a reaction mixture comprised of a palladium promoter dissolved in a high-concentration formic acid, wherein the concentration of the formic acid in the reaction mixture is ... 10/13/05 - 20050227313 - Polypeptide cleavage process A palladatepalladium-promoted hydrolytic polypeptide cleavage process which selectively cleaves the polypeptide at a Cys-His cleavage site comprising solubilizing the polypeptide in a reaction mixture comprised of a palladatepalladium promoter dissolved in a high-concentration acidic organic acid solvent. ... 10/13/05 - 20050227312 - Method for gene expression The invention relates to a method for gene expression in a cell-free transcription/translation system, the reaction solution containing all the components necessary for the transcription/translation mechanism, amino acids, nucleotides, metabolic components which provide energy and which are necessary for synthesis, and the proteins arising during translation being immobilized on a ... 09/29/05 - 20050214900 - Enzyme/tag binding and detection system A hybrid polypeptide composed of an identification peptide and a desired functional protein are produced by recombinant DNA techniques. A DNA expression vector is constructed that includes segments of DNA coding for the identification peptide and the desired functional protein. The identification peptide consists of a specific sequence of amino ... 09/29/05 - 20050214899 - Removal of n-terminal methionine from proteins by engineered methionine aminopeptidase The present invention provides methionine aminopeptidases (MetAPs) with a broad substrate range, particularly those capable of removing the N-terminal Met from bulky or acidic penultimate residues. In preferred embodiments, these MetAPs have mutations at the 233, 206 and/or 168 positions of SEQ ID NO:1. Preferably, amino acids at these residues ... 08/25/05 - 20050186657 - Glycoprotein synthesis Methods for making glycoproteins, both in vitro and in vivo, are provided. One method involves incorporating an unnatural amino acid into a protein and attaching one or more saccharide moieties to the unnatural amino acid. Another method involves incorporating an unnatural amino acid that includes a saccharide moiety into a ... 08/25/05 - 20050186656 - Glycoprotein synthesis Methods for making glycoproteins, both in vitro and in vivo, are provided. One method involves incorporating an unnatural amino acid into a protein and attaching one or more saccharide moieties to the unnatural amino acid. Another method involves incorporating an unnatural amino acid that includes a saccharide moiety into a ... 08/25/05 - 20050186655 - Preparation containing cell extracts for cell-free protein synthesis and means for synthesizing protein using the preparation Disclosed are a preparation containing cell extracts for cell-free protein synthesis, prepared by excluding from a living organism a system, participating to inhibiting of self protein synthesis reaction, an apparatus for cell-free protein synthesis reaction equipped with a reaction tank for cell-free protein synthesis, and a kit for use therefor; ... 08/25/05 - 20050186654 - Method for producing dipeptide A method for producing a dipeptide including the step of exposing a carboxy component and an amine component to an enzyme having an activity to hydrolyze amino acid ester. Such a hydrolase has been found among enzymes in bacteria. The method is useful for producing a peptide simply and inexpensively ... 08/18/05 - 20050181471 - Glycoprotein synthesis Methods for making glycoproteins, both in vitro and in vivo, are provided. One method involves incorporating an unnatural amino acid into a protein and attaching one or more saccharide moieties to the unnatural amino acid. Another method involves incorporating an unnatural amino acid that includes a saccharide moiety into a ... 08/11/05 - 20050176093 - Selective labeling agent for phosphoproteome analysis and phosphorylated site analysis Disclosed is a method of analyzing mass of the phosphoproteins or phosphopeptides and of analyzing phosphorylated positions at a phosphoprotein or phosphopeptide, comprising the steps of: 1) dephosphorylating at least one Ser and/or Thr residue of the phosphoprotein or phosphopeptide; 2) tagging the dephosphorylated amino acid residues with a tag ... 08/11/05 - 20050176092 - Regulation of gene expression by protein methylation The invention relates to the cDNA and deduced amino acid sequence of the Coactivator Associated arginine (R) Methyltransferase protein, CARM1. A method is described for the use CARM1 to regulate gene expression in vivo. CARM1 has also been used to methylate arginine residues of histones, synthetic peptides, and other proteins. ... 08/11/05 - 20050176091 - Process for producing kiss-1 peptide A KiSS-1 Peptide or a salt thereof can be industrially mass-produced by subjecting a fused protein or peptide in which the KiSS-1 peptide is ligated to the N-terminal of a low-molecular peptide having cysteine at its N-terminal to the cleavage reaction of the peptide linkage on the amino group side ... 08/04/05 - 20050170449 - Soluble glcnac phosphotransferase The present invention relates to a soluble GlcNAc phosphotransferase, a method of making the same and a method of phosphorylating with the same. ... 07/28/05 - 20050164340 - Bioactive whey protein hydrolysate The invention relates to a partial hydrolysate of when protein which contains bioactive peptides but does not have a bitter flavour. The hydrolysate is carried out using selective enzymes which produce the active peptides and is terminated at a degree of hydrolysis before substantial bitter flavours are created. There are ... 07/28/05 - 20050164339 - Compositions and methods for dehydration and cyclization of peptides, synthetic compounds, and lantibiotics Lantibiotics are synthesized on ribosomes as prepeptides and post-translationally modified to a mature form. These modifications include dehydrations and cyclizations. Compounds and related methods of generating compounds, modified by dehydration, cyclization, or dehydration and cyclization, are disclosed. The disclosure includes in vitro approaches to effecting dehydration and cyclization leading to ... 07/28/05 - 20050164338 - H. pylori fucosyltransferases This invention provides nucleic acid and amino acid sequences of fucosyltransferases from Helicobactor pylori. The invention also provides methods to use the fucosyltransferases to synthesize oligosaccharides, glycoproteins, and glycolipids. ... 07/28/05 - 20050164337 - Process for the extraction, purification and enzymatic modification of soy 7s globulin alpha' subunit for use as hypocholesterolemizing agent A process for the extraction, purification and enzymatic modification of β-conglycinin α′ subunit, wherein β-conglycinin is selectively extracted from ground, defatted soy, then precipitated by treatment with aqueous ethanol; the enriched fraction is then subjected to Metal Affinity Chromatography (MAC) in denaturant conditions to obtain the α′ subunit, which is ... 07/28/05 - 20050164336 - Method for protein expression analysis The present invention is a method for analysis of at least peptides comprising extracting proteins from at least one set of cells; digesting the extracted proteins; derivatising the protein fragment mixture with an isotopically labelled reagent molecule; separating the protein fragment mixture by multi-dimensional chromatography; and analysing the protein fragment ... 07/21/05 - 20050158820 - In vivo production of cyclic peptides The present invention relates ti methods and compositions utilizing inteins to generated libraries of cyclic peptides in vivo. ... 07/21/05 - 20050158819 - Bioarray chip reaction apparatus and its manufacture A body 300 having a cavity 310 for mounting a substrate 120 fabricated with probe sequences at known locations according to the methods disclosed in U.S. Pat. No. 5,143,854 and PCT WO 92/10092 or others, is provided. The cavity includes inlets 350 and 360 for introducing selected fluids into the ... 07/14/05 - 20050153390 - Lyophilized preparation for synthesis of cell-free protein Lyophilized preparations from cell extract for use in cell-free protein, which exhibit an activity for protein synthesis being in no way inferior to that exhibited in low-temperature storage; and a method of utilizing the same. In particular, preparations are formed by performing lyophilization after reducing the concentration of deliquescent substances ... 07/07/05 - 20050148050 - Bioadhesive composition comprising a polyphenolic protein A bioadhesive composition comprises a bioadhesive polyphenolic protein derived from a byssus-forming mussel and a polymer-comprising carbohydrate group, but does not include enzymatic oxidizing agents or chemical cross-linking agents. ... 07/07/05 - 20050148049 - Proteolytic fermenter A process of controlling the production of enzymatic protein hydrolysate from cold water animals, containing up to 90% free amino acids, cold fish oil, hydroxilaptite (calcium orthophosphate), proteins, peptides, vitamin, minerals and calcium salts of phosphorus. The process provides a greater degree of refining than existing technology through the use ... 07/07/05 - 20050148048 - Process for producing dipeptides The present invention provides a process for producing a dipeptide which comprises: allowing an enzyme source and one or more kinds of substances selected from the group consisting of amino acid amides, amino acid esters and amino acids to be present in an aqueous medium, said enzyme source being a ... 07/07/05 - 20050148047 - Beta1,4mannosyltransferases The unique function of the gene egghead as a GDP-mannose: Glcβ1 -Cer β1,4 mannosyltransferase is disclosed. The invention discloses isolated DNA molecules and DNA constructs encoding fragments of egghead and derivatives thereof by way of amino acid deletions, substitutions or insertions exhibiting egghead activity, as well as cloning and expression ... 07/07/05 - 20050148046 - Reaction solution for cell-free protein synthesis, method of preparing the same, and protein synthesis method using the same The present invention provides a cell-free protein synthesis method characterized by a cell-free protein synthesis reaction solution that is weakly reducing and suitable for protein folding, performing a weakly reducing synthesis reaction, and preferably performing a translation reaction with the further addition of a substance catalyzing a disulfide bond exchange ... 06/30/05 - 20050142633 - Recombinantly expressed carboxypeptidase b and purification thereof The invention provides a method to produce a protein with carboxypeptidase B activity from a pro-carboxypeptidase B zymogen, derived from a non-animal host organism. Carboxypeptidase B is activated from the zymogen using non-denaturing conditions. Particularly, the activation is performed under conditions that avoid unwanted non-covalent binding of the propeptide to ... 06/30/05 - 20050142632 - Neutral protease (np) and product by using neutral protease for tissue dissociation as well as method for the production thereof The use of a neutral protease (NP) together with a collagenase consists in that a neutral protease which is not contained in a collagenase enzyme preparation and which is not produced by a recombinant production ia mixed before the beginning of a tissue dissociation with a collagenase or a collagenase ... 06/23/05 - 20050136510 - Methods of preparing crosslinked materials and bioprosthetic devices Methods for making crosslinked material, such as collagen-based material, and bioprosthetic devices. The crosslinked material includes hydroxyl groups, carboxyl groups, and amine groups prior to crosslinking. The methods include removing at least a portion of the zero-length ester crosslinks in a crosslinked material and/or blocking at least a portion of ... 06/16/05 - 20050130261 - Process for crosslinking proteins with a ketose containing 3 to 5 carbon atoms The present invention relates to a process for cross-linking proteins, according to which the crosslinking agent is a ketose containing 3 to 5 carbon atoms and said proteins are chosen from the group comprising proteins from animal tissues, from milk or from blood, such as in particular casein, gelatin or ... 06/16/05 - 20050130260 - Refolded membrane protein in monodisperse form The present invention relates to a method for preparing a solution of a refolded, recombinantly expressed or chemically synthesized eukaryotic membrane protein in monodisperse form, to methods for preparing a crystalline form of a recombinantly expressed or chemically synthesized membrane protein, to a crystalline form of a recombinantly expressed, or ... 06/16/05 - 20050130259 - Expression vector, host, fused protein, process for producing fused protein and process for producing protein It is an object of the present invention to provide an expression vector, a host, a fused protein, a protein, a process for producing a fused protein, and a process for producing a protein, which can prevent formation of an unactive abnormal protein at production of a recombinant protein, and ... 06/16/05 - 20050130258 - Ordered biological nanostructures formed from chaperonin polypeptides The following application relates to nanotemplates, nanostructures, nanoarrays and nanodevices formed from wild-type and mutated chaperonin polypeptides, methods of producing such compositions, methods of using such compositions and particular chaperonin polypeptides that can be utilized in producing such compositions. ... 06/09/05 - 20050124035 - Method for producing alpha-l-aspartyl-l-phenylalanine-beta-ester and methd for producing alpha-l-aspartyl-l-phenylalanine-alpha-methyl esther A method for producing an α-L-aspartyl-L-phenylalanine-β-ester (also named α-L-(β-o-substituted aspartyl)-L-phenylalanine), which is an intermediate of an α-L-aspartyl-L-phenylalanine-α-methyl ester (also named α-L-aspartyl-L-phenylalanine methyl ester; product name: aspartame), easily, at high yield and inexpensively without going through a complex synthesis method is provided. Further, an easy, inexpensive and high-yield production method for ... 06/09/05 - 20050124034 - Method for producing fish gelatin peptide A method for producing gelatin peptide comprises the steps of cleaning fish skin with 0.1-5% salt water and fresh water, obtaining an extract from the fish skin using fresh water in neutral pH at 50-100° C., subjecting the water phase to enzymatic digestion, concentrating solution of the digest, activated carbon ... 06/02/05 - 20050118673 - Process for creating a 3-dimensional configuration on a substrate The invention provides a process for introducing a three-dimensional configuration of micron to sub-micron size in a polymeric substrate comprising applying a catalyst for the selective removal of sub-unit parts of the polymer to at least one predetermined area of the polymer substrate via a pipette with a nano-sized orifice. ... 06/02/05 - 20050118672 - Glycopeptide remodeling using amidases This invention provides methods for modifying glycosylation patterns of glycopeptides, including recombinantly produced glycopeptides. Also provided are glycopeptide compositions in which the glycopeptides have a homogeneous glycosylation pattern. ... ### FreshPatents.com Support |