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Chemistry: Molecular Biology And Microbiology > Measuring Or Testing Process Involving Enzymes Or Micro-organisms; Composition Or Test Strip Therefore; Processes Of Forming Such Composition Or Test Strip > Involving Hydrolase > Involving Proteinase Involving ProteinaseInvolving Proteinase patent applications listed are from June 2005 to current and include Date, Patent Application Number, Patent Title, Patent Abstract summary and are linked to the corresponding patent application page.03/22/07 - 20070065898 - Fluorescence polarization assays for binding of caspase inhibitors and probes therefor wherein the constituent variables are as defined herein. ... 03/22/07 - 20070065897 - Human prss11-like s2 serine protease and uses thereof A novel human cDNA, termed PRSS11-L, was isolated which encodes a polypeptide that belongs to the S2/HtrA serine protease family. The PRSS11-L mRNA is widely expressed in several tissues throughout the body by multi-tissue Northern blotting. The full-length PRSS11-L cDNA, was cloned, expressed and purified. Proteolytic activity was demonstrated using ... 03/22/07 - 20070065896 - Methods and kits for quantifying metabolites or analytes Methods and kits for quantifying the amount of a metabolite, such as homocysteine, methionine or cysteine, in a sample are disclosed. This involves contacting the sample with a first enzyme capable of degrading a metabolite into a plurality of reaction products and regenerating the metabolite using a second enzyme which ... 03/22/07 - 20070065895 - Substrates specific to von willebrand factor cleaving protease and method of assaying the activity The present invention relates to specific substrates for a von Willebrand factor cleaving enzyme, ADAMTS-13, as well as to diagnosis of ADAMTS-13 deficient patients, diagnostic compositions, and kits employing the substrates. Particularly preferable substrate polypeptides for ADAMTS-13 are the polypeptide which begins at amino acid 1587 and ends at amino ... 03/08/07 - 20070054346 - Method for measuring protease activity of transglutaminase and transglutaminase composition The invention provides a method for measuring the protease activity of a transglutaminase-containing product comprising: mixing an aqueous solution (sample solution) of a transglutaminase-containing product and an aqueous solution of dimethylcasein in a prescribed ratio of transglutaminase activity to quantity of dimethylcasein, allowing the mixture to stand under prescribed conditions ... 03/08/07 - 20070054345 - Expression quantification using mass spectrometry In various aspects, the present teachings provide systems, methods, assays and kits for the absolute quantitation of protein expression. In various aspects, the present teachings provide methods of determining the concentration of one or more proteins of interest in one or more samples of interest. In various aspects, the present ... 03/08/07 - 20070054344 - Novel fructosyl peptide oxidase and utilization thereof The present invention is directed to a defructosylation enzyme originating from a plant, a method of defructosylating a fructosylated peptide or protein through use of the enzyme, and a method of measuring a fructosylated peptide or protein. ... 03/01/07 - 20070048816 - Detection of proteases secreted from pathogenic microorganisms A diagnostic test kit for detecting the presence or absence of a protease (e.g., aminopeptidase) within a test sample is provided. The test kit comprises a substrate that is capable of being cleaved in the presence of the protease to release a compound. The kit also comprises a lateral flow ... 02/22/07 - 20070042452 - Detection of plasma ace2 A method of assessing a subject for aberrant cardiac function is described. The method particularly involves the measurement of a plasma biomarker, namely metallopeptidase angiotensin-converting enzyme 2 (ACE2), implicated in ischaemic heart disease. ... 02/15/07 - 20070037242 - On-line enzymatic digestion in separation-detection methods Apparatus and methods are disclosed for digesting a biopolymer in a medium. The medium is disposed adjacent a wall of a hollow element wherein at least a portion of the wall is porous. The medium is then exposed to digestion conditions to cleave the biopolymer into fragments. The fragments are ... 02/08/07 - 20070031913 - Amino-acid biosensor, fischer-ratio biosensor and health information management system Disclosed is a biosensor capable of measuring a total concentration of plural types of amino acids. An amino-acid biosensor (200) for measuring a total concentration of a plurality of specific amino acids, comprises a measuring electrode (202) which includes as components a mediator and an enzyme which selectively act on ... 02/08/07 - 20070031912 - Method for analysis of protein interaction using fluorescent protein An object of the present invention is to provide a method for analyzing the protein interaction, wherein the information about time can be obtained and the movement of protein can be monitored. The present invention provides a method for analyzing interaction between a first test protein and a second test ... 02/08/07 - 20070031911 - Isotopically-labeled proteome standards The invention provides methods for quantifying biomolecules, such as polypeptides in mass spectrometric analysis. The methods include use of a biomolecule standard having at least one atomic isotope different than that of the naturally occurring isotopes in the biomolecule of interest. Methods of the present invention also include methods for ... 02/08/07 - 20070031910 - Method of stabilizing sample A method for stabilizing a hemolyzed sample that does not affect an accuracy of determination in a measurement even after the sample is left standing at room temperature, and a hemolysis reagent solution used therefor are provided. Using a hemolysis reagent solution in which a surfactant is contained and carbon ... 02/01/07 - 20070026479 - Liquid protein markers for native gel electrophoresis Marker sets are provided for use on nondenaturing gels. The protein molecular weight markers are provided in liquid form, and are stable in liquid form for at least two months at 4 degrees C. and at least one year at −20 degrees C. Methods of using the markers and kits ... 02/01/07 - 20070026478 - Hemiasterlin affinity probes and their uses Photoaffinity probes are provided that are based hemiasterlin and derivative compounds thereof. Use of these probes to identity ending sites for these and other drugs, particularly anti-tubulin drugs, are also provided as are methods for identifying new drugs (e.g., new anti-tubulin drugs) that bind to these binding sites. ... 01/18/07 - 20070015234 - Whole cell assay for inhibitors of cathepsin k The present invention relates to a method of identifying and evaluating chemical inhibitors of cathepsin K activity in whole cells. The present invention also relates to probes that are capable of forming irreversible adducts with cathepsin K at the active site. The probes used in the instant invention comprise a ... 01/18/07 - 20070015233 - Analysis of molecules There is disclosed a method of analysing one or more molecules having a primary amine or a lysine functionality in two or more samples comprising the steps of: providing a series of reagents having the formula R1—NH—C(═NH)—OR2 where R1 and R2 are residue groups or atoms, and wherein the chemical ... 01/11/07 - 20070009982 - System for differential determination of a proteolytic enzyme level in a bodily fluid The system includes an assortment (10) of sensors (10a, 10b, 10c), the working electrode (14a) of each sensor being covered with a specific reagent of a given proteolytic enzyme, including a substrate capable of releasing leaving groups (LG) via the action of said enzyme, a measuring apparatus (20) having an ... 01/11/07 - 20070009981 - Nematode phosphoethanolamine n-methyltransferase-like sequences Nucleic acid molecules from nematodes encoding phosphoethanolamine n-methyltransferase polypeptides are described. PEAMT-like polypeptide sequences are also provided, as are vectors, host cells, and recombinant methods for production of PEAMT-like nucleotides and polypeptides. Also described are screening methods for identifying inhibitors and/or activators, as well as methods for antibody production. ... 01/11/07 - 20070009980 - Continuous fluorogenic analyte assays with dendritic amplification of signal Substrate compounds comprising a trigger moiety, a reporter system comprising a plurality of fluorescent moieties, and a multivalent self-immolative dendrimer linker linking the trigger moiety to the reporter system. ... 12/28/06 - 20060292660 - Preparations for diagnosis of pancreatic exocrine function A preparation for the diagnosis of pancreatic exocrine function by determining the amount in which a substance administered to a subject or a degradation product or metabolite thereof migrates into the blood and/or is excreted out of the body, wherein the substance is carried by a carrier and released from ... 12/28/06 - 20060292659 - Protease regulator screening assay A polypeptide containing an anchor region, a protease recognition site, and a detectable signal region can be produced recombinantly and directly attached to a solid support. The polypeptide is useful for screening protease regulators, especially protease inhibitors. ... 12/28/06 - 20060292658 - Fluorescent compound A water-soluble photoluminescent compound including a symmetrical skeletal structure of the formula (A) in which x may represent any integer, and in which the phenyl rings may be substituted, is disclosed. A protein detector comprising such a compound and a method of measuring the total dissolved protein content of a ... 12/28/06 - 20060292657 - Phex substrates and methods using same A fluorogenic PHEX substrate comprising a peptide unit; a fluorophore unit capable of conferring fluorescence on said substrate attached to an amino acid residue at a first end of the peptide unit; and a quencher unit capable of providing intrarnolecular quenching of said fluorescence attached to an amino acid residue ... 12/07/06 - 20060275857 - Enzyme sensor with a cover membrane layer covered by a hydrophilic polymer The present disclosure relates to an improved enzyme sensor comprising a cover membrane layer of a porous polymeric material, the outer surface and pore mouths of at least one face of the porous polymeric material being covered by a hydrophilic polymer. The sensor is useful determining the presence or amount ... 12/07/06 - 20060275856 - Method for analyzing cell free notch cleavage and method for drug screening The present invention provides a method for analyzing cell-free Notch cleavage which can be utilized for drug screening of γ-secretase inhibitors effective for the treatment of Alzheimer's disease and the drug screening method, is the method for analyzing cell-free Notch cleavage having a cell-free cleavage reaction step of contacting a ... 11/30/06 - 20060269981 - Diagnostic kit for hirschsprung disease (congenital megacolon) The present invention relates to a diagnostic kit for Hirschsprung disease comprising one or more appropriately stored, pre-measured histochemical enzymatic techniques. These techniques are selected from AChE, ANE, NADPH-d, SDH and LDH. In a second aspect thereof, this invention also relates to a diagnostic method for determining Hirschsprung disease. ... 11/30/06 - 20060269980 - Complete chemical and enzymatic treatment of phosphorylated and glycosylated proteins on protein chip arrays A simple and quick protocol for chemical treatment, enzymatic or chemical digestion, and subsequent identification of proteins on protein chip arrays is disclosed, together with kits therefor. The chemical treatment comprises denaturation, reduction and alkylation while enzymatic digestion encompasses deglycosylation, dephosphorylation, and digestion by various proteases. Digestion is also accomplished ... 11/30/06 - 20060269979 - High throughput glycan analysis for diagnosing and monitoring rheumatoid arthritis and other autoimmune diseases One can identify and quantify one or more glycosylation markers of an autoimmune disease such as rheumatoid arthritis by utilizing quantitative HPLC analysis of glycans which have been released from unpurified glycoproteins. The unpurified glycoproteins can be total glycoproteins or a selection of the total glycoproteins. The identified glycosylation marker ... 11/23/06 - 20060263842 - Methods and compositions for detection of microbial contaminants in peritoneal dialysis solution Methods and compositions for detection of microbial contaminants in peritoneal dialysis solutions are provided. The methods and compositions employ modified bioburden testing and the detection of peptidoglycan. A novel cause of aseptic peritonitis is provided—aseptic peritonitis associated with gram positive microbial contamination of a dialysis solution. Peptidoglycan is a major ... 11/09/06 - 20060252109 - Peptide conjugates and fluorescence detection methods for intracellular caspase assay Polypeptides labelled with a donor and acceptor pair of dyes selected from a dibenzorhodamine dye and a diamino-benzophenoxazine dye are peptide conjugates which are useful for intracellular and bead-based assays with fluorescence detection. Peptide conjugates with a caspase-recognition site undergo cleavage into peptide fragments which may be detected, located, and ... 11/02/06 - 20060246533 - Method and apparatus for performing peptide digestion on a microfluidic device The invention provides methods and apparatuses that allow a protein sample to undergo reduction, alkylation, and digestion in a continuous flow process carried out within a microfluidic device. Methods and apparatuses in accordance with the invention can be employed as part of an automated proteomics analysis carried out in an ... 11/02/06 - 20060246532 - Anthrax bioassays and methods of treating and diagnosing anthrax infection Based on the observation that exposure of cells or animals to anthrax lethal toxin results in activation of the intracellular enzyme caspase-1/IL-1 converting enzyme (ICE), which, in turn, leads to production and extracellular release of the cytokine substrates of ICE: interleukin 1 beta (IL-1β) and interleukin 18 (IL-18), disclosed herein ... 11/02/06 - 20060246531 - Chemo-enzymatic process for proteome-wide mapping of post-translational modification The invention provides a method for mapping the location of the post-translational modifications of a post-translationally modified peptide. Also provided is a solid-phase support that includes a reagent for modifying a post-translationally modified amino acid residues of a post-translationally modified, converting it into a substrate for a peptidase. ... 11/02/06 - 20060246530 - Method and reagent for the specifically identifying and quantifying one or more proteins in a sample The present invention relates to the MeCAT (Metal-chelate-complex-coded-affinity-tag)-method and to a reagent suitable for performing said method, which method includes a reproducible, systematic, qualitative and quantitative proteome characterization by means of non-isotope metal coded markers and—among other items—the most modem tandem methods of mass spectrometry. ... 10/26/06 - 20060240503 - Protease detection assay The invention provides methods and compounds for detecting protease activity in a sample solution comprising contacting the sample solution with a protease substrate labelled with an electrochemically active marker, providing conditions under which any protease which may be present in the sample may degrade the protease substrate and electrochemically determining ... 10/12/06 - 20060228768 - Method of judging the onset of periodontitis The inventor discovered that γ-GTP (γ-glutamyl transpeptidase) was scarcely contained in the gingival crevicular fluid of patients with gingivitis, but was contained in the gingival crevicular fluid of patients with periodontitis. Thus, the inventor provides a determination method of development of periodontitis by detecting or measuring γ-GTP contained in the ... 09/28/06 - 20060216779 - Method for the detection of post-translational modification activities and device system for carrying out said method The invention relates to a method for the qualitative detection of post-translational modification activities in a small liquid sample from organism cells or test chemicals. Phosphorylation/dephosphorylation of proteins by kinases and phosphatases is an example of a post-translational modification. The method is characterised in that protein fragments or polypeptides are ... 09/21/06 - 20060211078 - Caspase 9 activation and uses therefor The present invention discloses methods for activating Caspase 9 in such a way that it can be used in assays to discover modulators of Caspase 9. ... 09/21/06 - 20060211077 - Methods and compositions to diagnose disease using concatenated oligopeptide standard The present invention is a method to quantitate proteins associated with a disease or disease state comprising the steps of isolating at least two target proteins from a test sample, combining the target proteins with a known concentration of a concatenated oligopeptide standard and a proteinase to form peptide fragments ... 09/21/06 - 20060211076 - Method and device for the qualitative and/or quantitative determination of a protein and/or peptide pattern of a fluid sample, which has been taken from a human or animal body Provided are a process and a device for the qualitative and/or quantitative determination of a protein and/or peptide pattern of a liquid sample taken from a human or animal body for checking its state. The peptides and proteins of the liquid sample are processed and then subjected to analysis, wherein ... 09/07/06 - 20060199241 - Electrochemically and optically monitoring cleaving enzyme activity A marker molecule for monitoring cleaving enzyme activity is disclosed. The marker molecule includes a protein, a peptide, or an oligonucleotide. A co-factor is conjugated to the protein, the peptide, or the oligonucleotide, thereby forming a co-factor labeled protein, a co-factor labeled peptide, or a co-factor labeled oligonucleotide. The co-factor ... 08/31/06 - 20060194272 - Reference material for assaying d-dimer The present invention relates to a reference material for assaying D-dimer, comprising a fibrin degradation product, wherein no less than 70% of a total peak area has the molecular weight of no less than 500,000 in a molecular weight distribution of the fibrin degradation product, measured by gel filtration chromatography. ... 08/17/06 - 20060183180 - Methods for treating pervasive development disorders A method of utilizing the chymotrypsin level of an individual as a measure of the success of secretin, other neuropeptides, and peptides or digestive enzyme administration to such individuals, and in particular, as a prognosticative of potential secretin, other neuropeptides, peptides, and digestive enzyme administration for persons having ADHD, Autism ... 08/10/06 - 20060177887 - Method of analyzing a protein Provided a method of acquiring information on internal sequence which is applicable to a trace amount of protein and has a high reliability. A protein to be analyzed is limitedly cleaved at a specified position of amino acid to prepare a mixture of a plurality of peptide fragments; one or ... 08/10/06 - 20060177886 - Method for diagnosis of rheumatoid arthritis The present invention aims at providing a method for the diagnosis of RA. The present invention relates to a method for the diagnosis of rheumatoid arthritis, comprising measuring the dipeptidyl peptidase activity in a sample collected from a mammal. ... 08/03/06 - 20060172365 - Mass spectrometry The present invention is concerned with methods for the de novo sequencing of polypeptides from data obtained from mass spectrometry devices, particularly from (MS)n devices. ... 07/27/06 - 20060166301 - Stable chromogenic test reagent and its use in coagulation-diagnostic tests The present invention relates to a chromogenic test reagent which comprises a chromogenic peptide substrate and an inhibitor of fibrin polymerization, which is particularly suitable for being used in coagulation-diagnostic tests and which is distinguished by the fact that it exhibits a nigh degree of stability and/or a long shelf ... 07/20/06 - 20060160167 - Screening assay for enzymes involved in pathophysiology using seldi-tof mass spectrometry The invention provides methods for quantifying enzymatic activity of an enzyme with a known substrate. The methods employ SELDI-TOF mass spectrometry, and are suitable, in particular, for assaying aspects of the renin-angiotensin system. The methods may be utilized to assess and/or monitor biological conditions associated with the renin-angiotensin system prior ... 07/13/06 - 20060154325 - Synthesis of epoxide based inhibitors of cysteine proteases Epoxide based cysteine protease inhibitors containing peptide derivatives and methods for synthesizing them in sold phase are disclosed. Preferably, an epoxy succinyl “warhead” (for binding to the enzyme) is prepared, having two amino acid residues or residue-like structures on either side. Natural and non-natural amino acids may be used. The ... 07/13/06 - 20060154324 - Prostate-specific antigen probes for optical imaging This invention provides a novel probe, which contains a prostate peptide substrate. The probe can be used as a prostate diagnostic agent for optical imaging. An 11 amino acid peptide substrate is synthesized for specific prostate-specific antigen (PSA). This protected graft copolymer (PGC) consists of a poly-L-lysine (PL) backbone and ... 07/13/06 - 20060154323 - Method for obtaining dynamic and structural data pertaining to proteins and protein/ligand complexes This invention provides an NMR method for obtaining both entropic and enthalpic data on proteins and protein/ligand complexes which can be used to obtain accurate structural and dynamic data of proteins and protein complexes having a wide range of molecular weights. An embodiment of the invention provides proteins which contain ... 07/06/06 - 20060148023 - Affinity labeling of serine proteases for simultaneous detection of multiple serine protease activity levels The invention provides assay methods and reagents useful for evaluating the level of enzyme activities within living cells. Enzyme activity levels within living cells, such as Serine proteases, can be key determinates in assessing; 1) the presence of tumor (cancer) cells, 2) the predictive efficacy of a chemotherapeutic treatment regimen ... 07/06/06 - 20060148022 - Assay for tissue factor and factor viia The invention provides assays for detecting and quantitating tissue factor and factor VIIa in simple and complex biological systems. The assays are performed by detecting and/or measuring the tissue factor cofactor activity and factor VIIa enzymatic activity using aminonapthalene-based fluorogenic substrates. ... 07/06/06 - 20060148021 - Extraction of a proteinaceous macromolecule from a biological sample The invention provides a method of treating a biological sample to extract a macromolecule under acidic conditions. In one aspect the present invention provides a method for enhancing the solubilisation of at least one proteinaceous macromolecule in a biological sample without inducing substantial acid hydrolysis of said proteinaceous macromolecule, said ... 06/29/06 - 20060141552 - Regulation of human caspase-1-like protease Screening methods to identify candidate therapeutic agents using a newly identified human caspases-1-like protease. ... 06/22/06 - 20060134723 - N-or c-terminal peptide selection method for proteomics The present invention provides a new and improved method for quantitative determination and/or identification of proteins in a sample. In one aspect, the invention provides a mass spectroscopic method for comparing protein levels in two or more samples by differentially isotopically labeling each sample's proteins' N- or C-termini. In another ... 06/22/06 - 20060134722 - Method and compositions for detecting botulinum neurotoxin A molecular construct capable of fluorescent resonance energy transfer (FRET), comprising a linker peptide, a donor fluorophore moiety and an acceptor fluorophore moiety, wherein the linker peptide is a substrate of a botulinum neurotoxin selected from the group consisting of synaptobrevin, syntaxin and SNAP-25, or a fragment thereof capable being ... 06/22/06 - 20060134721 - Affinity trap reactor and single-step process for purifying angiostatin-like fragment from human plasma using the same The present invention provides an affinity trap reactor that enables the reaction between an enzyme bound to a support and substrate to proceed efficiently without its applications being restricted by the type of enzyme and substrate used. The present invention relates to an affinity trap reactor composed of a support ... 06/22/06 - 20060134720 - Method for analyzing c-terminal amino acid sequence of peptide using mass spectrometry The present invention provides a method for analyzing the C-terminal amino acid sequence of a peptide by using a reaction for successively releasing the C-terminal amino acids of the peptide, which method can suppress, when successively releasing the C-terminal amino acids of a peptide of long amino acid length, such ... 06/22/06 - 20060134719 - Novel serine protease It is disclosed that a novel polypeptide useful for screening for an agent for specific diseases to which gastrointestinal hormones are related, particularly irritable bowel syndrome, a polynucleotide encoding the polypeptide, a vector comprising the polynucleotide, and a transfected cell by the vector. It is disclosed that the polypeptide is ... 06/15/06 - 20060127965 - Bindingzyme arrays and high-throughput proteomic methods Provided herein are methods for identifying the presence or absence of a polypeptide variance between different biological samples and corresponding methods for generating a high-throughput screen to rapidly identify variances of one or more polypeptides in different biological samples. In particular, a variance in a post-translational modification on a particular ... 06/08/06 - 20060121553 - Fluorogenic enzyme assay methods, kits and compositions using charge-balancers Fluorescent compositions, methods and kits useful for, among other things, detecting, quantifying and/or characterizing enzymes. ... 06/08/06 - 20060121552 - Apparatuses and methods for determining protease activity The present invention relates to compositions and an apparatuses for determining protease activity. The compositions of the invention contain a reporter protein fused to at least one protease cleavage sequence, and a linker for attaching the protease cleavage sequence to a solid support. Methods for determining protease activity and characterizing ... 06/08/06 - 20060121551 - Novel phenoxazinone derivatives as enzyme substrates and use thereof as indicator in the detection of microorganisms with peptidase activity where R1, R2, R3, R4, R5, R6, A and X are as defined in claim 1, the reaction media comprising the same and the use thereof for the detection and/or identification and/or quantification of microorganisms expressing at least one peptidase activity. ... 06/01/06 - 20060115871 - Protein interaction reporter agents and methods for using same Particular aspects provide novel protein interaction reporter (PIR) compounds (e.g., formulas I and II), comprising at least two protein reactive moieties (e.g., N-hydroxysuccinamide), each linked to a reporter moiety (e.g., mass reporter) by a covalent labile bond that is differentially cleavable with respect to peptide bonds (e.g., by a method ... 05/25/06 - 20060110785 - Methods to differentiate protein conformers The invention is directed to methods to distinguish among different protein conformers of the same protein such as proteins which form amyloid deposits. Using the methods of the invention, one or more protein conformers in a sample can be detected, differentiated, and quantitated. An example of a protein which is ... 05/25/06 - 20060110784 - Site-specific labeling of proteins for nmr studies Methods of producing and/or analyzing spectroscopically labeled proteins, e.g., proteins site-specifically labeled with NMR active isotopes, spin-labels, chelators for paramagnetic metals, and the like, are provided. The labeled proteins are produced in translation systems including orthogonal aminoacyl tRNA synthetase/tRNA pairs. Methods for assigning NMR resonances, e.g., methods using isotopically labeled ... 05/18/06 - 20060105416 - Methods, compositions and kits pertaining to analyte determination This invention pertains to methods, kits and/or compositions for the determination of analytes by mass analysis using unique labeling reagents or sets of unique labeling reagents. The labeling reagents can be isomeric or isobaric and can be used to produce mixtures suitable for multiplex analysis of the labeled analytes. ... 05/18/06 - 20060105415 - Method for single oxygen atom incorporation into digested peptides using peptidases Optimized enzymatic conditions incorporate a single oxygen atom into digested peptides using a peptidase. The incorporation of a single oxygen atom is especially useful for proteolytic 18O labeling in comparative proteomics. The optimized proteolytic 18O labeling minimizes the generation of a mixture of isotopic isoforms of the peptides resulting from ... 04/27/06 - 20060088901 - Methods and kits for determining ubiquitin protein ligase (e3) activity The present invention provides a method and an assay kit for detecting ubiquitin ligase (E3) activity in a sample. The method includes the steps of (1) preparing a sample mixture comprising ubiquitin, a ubiquitin activating enzyme (E1), a ubiquitin conjugating enzyme (E2), a reaction buffer, and the sample; (2) incubating ... 04/20/06 - 20060084131 - Method for collecting information on the primary structure of proteins, hydrophobic microparticulate carrier used in same, and system for automatically obtaining information on the primary structure of proteins A method for obtaining information on the primary structure of peptides comprising fragmenting the proteins, which enables automation of obtaining information on the primary structure of a peptide sample; a device for use in the method; and an automatic analysis system on the primary structure of the peptides. The method ... 04/20/06 - 20060084130 - Method for diagnosing a transmissible spongiform subacute encephalopathy caused by an unconventional transmissible agent strain in a biological sample The invention relates to a method for diagnosing a transmissible spongiform subacute encephalopathy (TSSE) caused by an unconventional transmissible agent (UTA) or prion. The method involves treating a sample suspected of containing a prion with proteinase K for a time and under conditions that completely degrade normal prion protein (Prp-sen), ... 03/30/06 - 20060068457 - Substrates for tafi (a) The invention relates to compounds of general formula (I) and acid addition salts thereof, where the various symbols have the meanings given in the description and claims, the production and use thereof as substrate for the detection of TAFIa, a fibrinolysis inhibiting enzyme. The detection occurs by using the absorption ... 03/23/06 - 20060063223 - Enzymatic activities in chemokine-mediated inflammation Truncated chemokines lacking an N-terminal region that activate CCR1 and/or FPRL1 and compositions containing the truncated chemokines are provided. Methods of identifying agents that modulate CCR1 and/or FPRL1 activity either by modulating the production of the truncated chemokines or the ability of the truncated chemokines to activate CCR1 and/or FPRL1 ... 03/23/06 - 20060063222 - Fluorescence polarization assays for determining clostridial toxin activity The present invention provides a method of determining the presence or activity of a clostridial toxin by (a) treating with a sample, under conditions suitable for clostridial toxin protease activity, a clostridial toxin substrate which includes a fluorophore; a bulking group; and a clostridial toxin recognition sequence containing a cleavage ... 03/23/06 - 20060063221 - Lanthanide-based substrates and methods for determining clostridial toxin activity The present invention provides a clostridial toxin substrate that contains (a) a lanthanide donor complex; (b) an acceptor having an absorbance spectrum overlapping the emission spectrum of the lanthanide donor complex; and (c) a clostridial toxin recognition sequence containing a cleavage site that intervenes between the lanthanide donor complex and ... 03/16/06 - 20060057660 - Method for measuring protein nmr An object of the present invention is to provide a means for highly efficiently or quickly evaluating or determining the three-dimensional structure of a protein by conducting multidimensional multinuclear NMR measurement without performing a step of separating and purifying the synthesized labeled protein from other labeled proteins. The present invention ... 03/16/06 - 20060057659 - Destructible surfactants and uses thereof The present invention provides methods for enhancing chemical reactions of molecules, e.g., biomolecules, with destructible surfactants. The chemical reactions may involve and/or be associate with analysis, e.g., solubilizing, separating, purifying and/or characterizing the molecules. In one aspect, the anionic surfactants of the present invention may be selectively broken up at ... 03/09/06 - 20060051831 - Method and kit for quantitative analysis of protein The present invention provides a method for global quantitative analysis of protein that is effectively applied also for unpurified samples such as biological samples, and achieves better detection sensitivity and quantitativeness than the conventional NBS method. A method for global quantitative analysis of protein comprising: preparing two states of protein ... 02/23/06 - 20060040335 - Diagnostic and screening methods and kits associated with proteolytic activity Methods and kits for assessing proteolytic enzyme activity and a modulator's effect thereof employ a Ubiquitin or Ubiquitin-like Protein and a signal producing structure. The corresponding fusion polynucleotide is employed for production of transgenic cells, plants and animals that may be produced by stably transfection, optionally transforming the cell, plant ... 02/23/06 - 20060040334 - Method for characterising analytes Provided is a method for characterising an analyte by matrix assisted laser desorption ionisation (MALDI) mass spectrometry, which method comprises: (a) labelling the analyte with a light-absorbing label that absorbs light at a pre-determined frequency, to form a labelled analyte; (b) embedding the labelled analyte in a matrix formed from ... 02/16/06 - 20060035307 - Method and apparatus for the detection of an enzyme An apparatus for detecting pepsin comprising a solid support, and a peptide chain wherein the peptide chain is operatively configured to be cleaved by pepsin, and the peptide chain is disposed on a surface of the solid support. ... 02/16/06 - 20060035306 - Enzyme substrates, culture media containing same and use thereof in order to detect aminopeptidase activity and/or differentiate gram-positive bacteria from gram-negative bacteria R8 consists of H or Cl, and n is an integer corresponding to 0 or 1. The invention is particularly suitable for use in the field of diagnostics. ... 02/09/06 - 20060029993 - Monitoring heart failure An in vitro method for identifying subjects at risk of heart failure following a Myocardial Infarction comprising analysing the concentration of Matrix Metalloproteinase-9 in a bodily sample from a subject who has suffered a Myocardial Infarction and comparing this concentration with a reference for concentrations of Matrix Metalloproteinase-9 in individuals ... 01/19/06 - 20060014234 - Method for the enrichment and characterization of phosphorylated peptides or proteins A method for the separation and assay of phosphorylated peptides or proteins from complex mixtures of proteins and peptides is described. The method uses a diazo moiety linked by an organic group to a substrate. Kits for performing the assay are also described. The method and kits are particularly useful ... 01/19/06 - 20060014233 - Methods for measuring adamts13 activity and protein on platelets and in plasma Provided are assays to detect ADAMTS13 activity using peptide substrates. These assays can be used for diagnostic applications and to evaluate treatment of thrombotic thrombocytopenic purpura (TTP). Also provided is a novel form of ADAMTS13 found on platelets. ... 01/19/06 - 20060014232 - Immobilization method Various biomolecules can be firmly immobilized to a substrate by a method which comprises contacting a solution containing biomolecules provided with at least one tag with an immobilization substrate which has (i) binding sites for the biomolecule tag or tags, and (ii) activated reactive groups which are capable of forming ... 01/12/06 - 20060008862 - Real time methylumbelliferone-based assay A method is provided for determining the activity of an enzyme which releases methylumbelliferone (MU) from an MU-containing substrate wherein the enzyme has a pH optimum below the pKa of MU comprising: contacting a sample suspected of containing the enzyme with the MU-containing substrate at a pH suitable for activity ... 01/05/06 - 20060003399 - High throughput actin polymerization assay Methods for assaying for actin polymerization are described, as are the use of the assays to screen for modulators of actin polymerization. The screening methods can be utilized to identify active agents that interact with actin, the polymerization state of actin and other proteins or cellular components whose function is ... 12/29/05 - 20050287622 - High throughput assays for the proteolytic activities of clostridial neurotoxins In this application is described substrates for high-throughput assays of clostridial neurotoxin proteolytic activities. Two types of substrates are described for use in assays for the proteolytic activities of clostridial neurotoxins: (1) modified peptides or proteins that can serve as FRET substrates and (2) modified peptides or proteins that can ... 12/22/05 - 20050282240 - Method for identifying peptides in a biological sample The invention provides a method for isolating a cargo peptide from a sample containing a cargo peptide-carrier protein complex. The method involves contacting a sample comprising a cargo peptide-carrier protein complex with a binding moiety selective for the carrier protein, under conditions wherein the carrier protein binds non-covalently to the ... 12/15/05 - 20050277165 - Novel treatment A method of screening compounds to identify those compounds which inhibit the Asp 2 mediated cleavage of a polypeptide or protein substrate, the method comprising: providing a reaction system comprising Asp 2 and substrate; and measuring the extent of cleavage of the substrate in the presence of test compound as ... 12/08/05 - 20050272113 - Saliva test for hemolytic streptococcus A reagent for the detection of an extracellular enzymatically active protein produced by a beta-hemolytic streptococcus bacteria found in a host biological fluid includes a proteinaceous substrate or a cholesterol-containing membrane substrate for the extracellular protein. The substrate is nonspecific within the groups of beta-hemolytic streptococcus bacterium and is in ... 12/01/05 - 20050266513 - Method for identifying compounds that inhibit the activity of myc Method for identifying compounds useful for the therapy of cancers in which the activity of Myc is causally involved. The method is based on measuring the activity of the E3 ligase HectH9, which is required for ubiquitination and activation of Myc. ... 12/01/05 - 20050266512 - Detection of proteases and screening for protease inhibitors The present disclosure provides a method for detecting a protease, which is simple, sensitive, and capable of high throughput. The method detects a protease in a sample by measuring lysis of a liposome due to activation of a modified or inactive channel-forming agent. Also disclosed is a method for screening ... 11/24/05 - 20050260697 - Proteolytic markers as diagnostic biomarkers for cancer, organ injury and muscle rehabilitation/exercise overtraining The present invention identifies biomarkers that are diagnostic of nerve cell injury, organ injury, and/or neuronal disorders. Detection of different biomarkers of the invention are also diagnostic of the degree of severity of nerve injury, the cell(s) involved in the injury, and the subcellular localization of the injury. ... 11/10/05 - 20050250171 - Methods and tools for detecting collagen degradation Fluorophore-labeled collagen covalently bound to a cell culture vessel can be used to assay collagen degradation by a variety of cell types, including osteoclasts and tumor cells. Such assays provide a high throughput platform for rapid screening of large numbers of potential modulators of, for example, tumor metastasis or osteoclast ... 10/20/05 - 20050233406 - Methods for high resolution identification of solvent accessible amide hydrogens in polypeptides and for characterization of polypeptide structure The present invention provides methods of determining, at a resolution of about 1-5 amino acid residues, the position of a peptide amide hydrogen that has been labeled with an isotope of hydrogen other than 1H by determining the quantity of isotope and/or rate of exchange of peptide amide hydrogen(s) with ... 10/20/05 - 20050233405 - Inhibitors of caspase-3-mediated cleavage of essential ventricular myosin light chain The invention discloses use of peptide containing an essential ventricular myosin light chain type 1 (vMLC1) amino acid sequence, which is functional as cleavage site for caspase-3, in the screening for a compound for the treatment of chronic or acute cardiovascular disease. Further, screening methods for inhibitors of the caspase-3-mediated ... 10/13/05 - 20050227306 - Fluorescence polarization assay to detect protease cleavage The invention discloses a method of determining protease activity, in real time, using fluorescence polarization technology. In particular, the invention provides vectors and a method for their use, which expresses uncharacterized proteins conjugated to a fluorescence tag, which binds specifically to a fluorescent ligand. Cleavage of the recombinant protein results ... 10/06/05 - 20050221414 - Kit for measuring the thrombin generation in a sample of a patient's blood or plasma The invention provides a kit for measuring the thrombin generation in a sample of a patient's blood or plasma, or in a sample of clotting factors. The kit contains lyophilized tissue factor/phospholipid-complex and a lyophilized mixture containing a thrombin-substrate and CaCl2. The invention also provides processes for preparing the reagents ... 10/06/05 - 20050221413 - Sulfenyl compound, labeling reagent, and method of analyzing peptide A sulfenyl compound represented by the general formula: R—S—X (I)(wherein R represents an organic group having at least one constituent element labeled with an isotope, and X represents a leaving group); a labeling reagent comprising it; and a method of analyzing peptide using the labeling reagent. Preferably the organic group ... 09/29/05 - 20050214890 - Novel cleave-n-read system for protease activity assay and methods of use thereof The present invention provides a reliable protease activity assay system for determination of cleavage of more than one recognition/cleavage site in a single assay. The assay relies on use of a fluorescent fusion substrate which comprises a purification module (PM), a first fluorescent protein (FP1), a specific protease recognition/scission site ... 09/29/05 - 20050214889 - Apparatus for measuring biodegradability of sample using non-dispersive infrared spectrometry and method of measuring the same Disclosed is an apparatus for measuring biodegradability of a polymer sample by non-dispersive infrared spectrometry, including a compression pump (2), a first air controlling unit (4), carbon dioxide removing devices (6), a filter (8), a first cooling device (10), at least two composting vessels (12), at least two second cooling ... 09/08/05 - 20050196824 - Chaperonin and osmolyte protein folding and related screening methods The invention describes an inexpensive in vitro protein folding process for preventing large scale protein misfolding and aggregation, for concentrating aggregation prone chaperonin-protein folding intermediates in a stable non-aggregating form, and for rapidly screening these stable concentrates for the best folding solution conditions. The process comprises: (1) the formation of ... 09/08/05 - 20050196823 - Methods and apparatus for gel-free qualitative and quantitative proteome analysis, and uses therefore Methods and apparatus for qualitative and quantitative proteome analysis are provided. The methods and apparatus allow for the isolation of a subset of peptides out of complex mixtures of peptides. The isolation is based on a specific chemical and/or enzymatic alteration of one or more types of peptides. This alteration ... 08/25/05 - 20050186649 - Inhibitors of human plasmin derived from the kunitz domains This invention provides: novel proteins, which are homologous to the first Kunitz domain (K1) of lipoprotein-associated coagulation inhibitor (LACI), and which are capable of inhibiting plasmin; uses of such novel proteins in therapeutic, diagnostic, and clinical methods; and polynucleotides that encode such novel proteins. ... 08/18/05 - 20050181469 - Beta-lactamase substrates having phenolic ethers in which R is a benzyl, 2-thienylmethyl, or cyanomethyl group; R′ is selected from the group consisting of H, physiologically acceptable salts or metal, ester groups, ammonium cations, —CHR2OCO(CH2)nCH3, —CHR2OCOC(CH3)3, acylthiomethyl, acyloxy-alpha-benzyl, deltabutyrolactonyl, methoxycarbonyloxymethyl, phenyl, methylsulphinylmethyl, β-morpholinoethyl, dialkylaminoethyl, and dialkylaminocarbonyloxymethyl, in which R2 is selected from the group consisting of ... 08/18/05 - 20050181468 - Biological markers and diagnostic tests for angiotensin converting enzyme inhibitor and vasopeptidase inhibitor-associated angioedema Deficiencies in certain physiological pathways are linked with ACE or vasopeptidase inhibitor associated angioedema. Additionally, detection and/or measurement of dipeptidyl peptidase IV (DPP IV) enzyme activity and aminopeptidase P (APP) enzyme activity is a predictor of this risk. The present invention provides biological markers, diagnostic tests, and pharmaceutical indications that ... 08/11/05 - 20050176085 - Method of selective peptide isolation for the identification and quantitative analysis of proteins in complex mixtures The present invention describes a method of selective peptide isolation for the identification and quantitative analysis of proteins in complex mixture. The method comprises the selective isolation from every protein of those peptides that neither contain arginine nor histidine (NHNR peptides), and the determination of the relative concentrations of one ... 07/28/05 - 20050164327 - Beta-secretase enzyme compositions and methods Disclosed are various forms of an active, isolated β-secretase enzyme in purified and recombinant form. This enzyme is implicated in the production of amyloid plaque components which accumulate in the brains of individuals afflicted with Alzheimer's disease. Recombinant cells that produce this enzyme either alone or in combination with some ... 07/28/05 - 20050164326 - Labeling of proteomic samples during proteolysis for quantitation and sample multiplexing This invention related to methods useful in the labeling of multiple polypeptide samples and subsequent analysis of these samples by mass spectrometry, particularly in the high throughput proteomic setting. ... 07/28/05 - 20050164325 - Method of mass spectrometry A method of identifying post-translationally modified proteins is disclosed. The method comprises mass analysing peptide ions and then subtracting from the determined mass of the peptide ion the known increase in mass due to one or more modifications of interest. The resulting value which represents the mass a peptide would ... 07/28/05 - 20050164324 - Systems, methods and kits for characterizing phosphoproteomes The invention provides systems, software, methods and kits for detecting and/or quantifying phosphorylatable polypeptides and/or acetylated polypeptides in complex mixtures, such as a lysate of a cell or cellular compartment (e.g., such as an organelle). The methods can be used in high throughput assays to profile phosphoproteomes and to correlate ... 07/14/05 - 20050153384 - Identifying inhibitors of intracellular protein fibrillization Methods for identifying and characterizing inhibitors of protein filament formation are provided. These methods are particularly useful for identifying those agents which inhibit or prevent protein filament formation within neurons of mammalian subjects, particularly human subjects, such as the formation of tau filaments in Alzheimer's patients, and α-synuclein filaments in ... 07/14/05 - 20050153383 - Synthetic and recombinant substrates for the detecion of the von willebrand factor-cleaving protease A method of detecting von Willebrand Factor-cleaving protease in a test sample is disclosed. In one embodiment, the method comprises the steps of (a) obtaining a test bodily fluid sample; (b) exposing the test sample to a monomeric von Willebrand Factor fragment, wherein the fragment comprises amino acids 842 and ... 06/23/05 - 20050136504 - Method of measurement of protease and thin membranes used for said method A method for measuring protease such as matrix metalloproteinase which comprising steps of (1) bringing a biological sample such as protease-containing tissue slices and tissue extracts into contact with a thin membrane which comprises a protease substrate and a hardening agent and is formed on a surface of a support; ... 06/02/05 - 20050118665 - Methods for conducting assays for enzyme activity on protein microarrays The present invention relates to methods of conducting assays for enzymatic activity on microarrays useful for the large-scale study of protein function, screening assays, and high-throughput analysis of enzymatic reactions. The invention relates to methods of using protein chips to assay the presence, amount, activity and/or function of enzymes present ... 06/02/05 - 20050118664 - Fluorogenic protease substrates Fluorogenic protease substrates are provided that comprise a peptide doubly labelled via thiol groups of the peptide with an alkyleneamidotetramethylrhodamine (alkyleneamido-TMR) group. Preferred substrates are doubly labelled with substantially pure 5-methyleneamido-TMR or 6-methyleneamido-TMR. Methods of preparing the substrates are provided, which comprise reacting the unlabelled peptide with haloalkylamido-TMR (preferably iodoacetamido-TMR). ... ### FreshPatents.com Support |