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Very large scale immobilized polymer synthesisRelated Patent Categories: Chemical Apparatus And Process Disinfecting, Deodorizing, Preserving, Or Sterilizing, Chemical Reactor, Bench ScaleVery large scale immobilized polymer synthesis description/claimsThe Patent Description & Claims data below is from USPTO Patent Application 20060210452, Very large scale immobilized polymer synthesis. Brief Patent Description - Full Patent Description - Patent Application Claims
CROSS REFERENCE TO RELATED APPLICATIONS [0001] This application is a continuation-in-part of pending application Ser. No. 492,462, filed Mar. 7, 1990, which is a continuation-in-part of pending application Ser. No. 362,901, filed Jun. 7, 1989, assigned to the assignee of the present application, and incorporated herein by reference for all purposes. This application is related to applications with Ser. Nos. ______ and ______, (Attorney Docket Nos. 11509-26 and 11509-30) filed on the same day as the present application, and also incorporated herein by reference for all purposes. COPYRIGHT NOTICE [0002] A portion of the disclosure of this patent document contains material which is subject to copyright protection. The copyright owner has no objection to the facsimile reproduction by anyone of the patent document or the patent disclosure as it appears in the Patent and Trademark Office patent file or records, but otherwise reserves all copyright rights whatsoever. BACKGROUND OF THE INVENTION [0003] The present invention relates to the field of polymer synthesis. More specifically, the invention provides a reactor system, a masking strategy, photoremovable protective groups, data collection and processing techniques, and applications for light directed synthesis of diverse polymer sequences on substrates. SUMMARY OF THE INVENTION [0004] Methods, apparatus, and compositions for synthesis and use of techniques for diverse polymer sequences on a substrate are disclosed, as well as applications thereof. [0005] According to one aspect of the invention, an improved reactor system for synthesis of diverse polymer sequences on a substrate is provided. According to this embodiment the invention provides for a reactor for contacting reaction fluids to a substrate; a system for delivering selected reaction fluids to the reactor; a translation stage for moving a mask or substrate from at least a first relative location relative to a second relative location; a light for illuminating the substrate through a mask at selected times; and an appropriately programmed digital computer for selectively directing a flow of fluids from the reactor system, selectively activating the translation stage, and selectively illuminating the substrate so as to form a plurality of diverse polymer sequences on the substrate at predetermined locations. [0006] The invention also provides a technique for selection of linker molecules in VLSIPS. According to this aspect of the invention, the invention provides a method of screening a plurality of linker polymers for use in binding affinity studies. The invention includes the steps of forming a plurality of linker polymers on a substrate in selected regions, the linker polymers formed by the steps of recursively: on a surface of a substrate, irradiating a portion of the selected regions to remove a protective group, and contacting the surface with a monomer; contacting the plurality of linker polymers with a ligand; and contacting the ligand with a labeled receptor. [0007] According to another aspect of the invention, improved photoremovable protective groups are provided. According to this aspect of the invention a compound having the formula: wherein n=0 or 1; Y is selected from the group consisting of an oxygen of the carboxyl group of a natural or unnatural amino acid, an amino group of a natural or unnatural amino acid, or the C-5' oxygen group of a natural or unnatural deoxyribonucleic or ribonucleic acid; R.sup.1 and R.sup.2 independently are a hydrogen atom, a lower alkyl, aryl, benzyl, halogen, hydroxyl, alkoxyl, thiol, thioether, amino, nitro, carboxyl, formate, formamido, sulfido, or phosphido group; and R.sup.3 is a alkoxy, alkyl, aryl, hydrogen, or alkenyl group is provided. [0008] The invention also provides improved masking techniques for VLSIPS. According to one aspect of the masking technique, the invention provides an ordered method for forming a plurality of polymer sequences by sequential addition of reagents comprising the step of serially protecting and deprotecting portions of the plurality of polymer sequences for addition of other portions of the polymer sequences using a binary synthesis strategy. [0009] Improved data collection equipment and techniques are also provided. According to one embodiment, the instrumentation provides a system for determining affinity of a receptor to a ligand comprising: means for applying light to a surface of a substrate, the substrate comprising a plurality of ligands at predetermined locations, the means for applying directing light providing simultaneous illumination at a plurality of the predetermined locations; and an array of detectors for detecting light fluoresced at the plurality of predetermined locations. The invention further provides for improved data analysis techniques including the steps of exposing fluorescently labelled receptors to a substrate, the substrate comprising a plurality of ligands in regions at known locations; at a plurality of data collection points within each of the regions, determining an amount of light fluoresced from the data collection points; removing the data collection points deviating from a predetermined statistical distribution; and determining a relative binding affinity of the receptor to remaining data collection points. [0010] Protected amino acid N-carboxy anhydrides for use in polymer synthesis are also disclosed. According to this aspect of the invention provides a compound having the formula: where R is a side chain of a natural or unnatural amino acid and X is a photoremovable protecting group. [0011] A further understanding of the nature and advantages of the inventions herein may be realized by reference to the remaining portions of the specification and the attached drawings. BRIEF DESCRIPTION OF THE DRAWINGS [0012] FIG. 1 schematically illustrates light-directed spatially-addressable parallel chemical synthesis; [0013] FIG. 2 schematically illustrates one example of light-directed peptide synthesis; [0014] FIG. 3 is a three-dimensional representation of a portion of the checkerboard array of YGGFL and PGGFL; [0015] FIG. 4 schematically illustrates the software for the automated system for synthesizing diverse polymer sequences; [0016] FIGS. 5a and 5b illustrate operation of a program for polymer synthesis; [0017] FIGS. 6a and 6b are schematic illustrations of a "pure" binary masking strategy; [0018] FIGS. 7a and 7b are schematic illustrations of a gray code binary masking strategy; [0019] FIGS. 8a and 8b are schematic illustrations of a modified gray code binary masking strategy; Continue reading about Very large scale immobilized polymer synthesis... 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