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  Therapy for cellular accumulation in chronic inflammatory diseasesUSPTO Application #: 20080044465Title: Therapy for cellular accumulation in chronic inflammatory diseases Abstract: The present invention provides a novel method for the treatment of cellular accumulation in chronic inflammatory diseases such as rheumatoid arthritis. The method includes gene delivery and gene expression that is capable of enhancing apoptosis of accumulating cells and those cells which recruit accumulating cells. Also provided are diagnostic methods for detecting cellular accumulation diseases. (end of abstract)
Agent: Dla Piper US LLP - San Diego, CA, US Inventors: Gary S. Firestein, Nathan J. Zvaifler, Douglas R. Green USPTO Applicaton #: 20080044465 - Class: 424450000 (USPTO) Related Patent Categories: Drug, Bio-affecting And Body Treating Compositions, Preparations Characterized By Special Physical Form, Liposomes The Patent Description & Claims data below is from USPTO Patent Application 20080044465. Brief Patent Description - Full Patent Description - Patent Application Claims
[0001] The present invention claims priority under 35 U.S.C. 119 from Provisional Application Ser. No. 60/002,948 and from Provisional Application Ser. No. 60/016,316 both of which are hereby incorporated herein in their entirety including all figures, drawings, graphs, illustrations, and equations. FIELD OF THE INVENTION [0003] The present invention relates generally to programmed cell death (apoptosis) and more specifically to the diagnosis and treatment of diseases of excess cellular accumulation due to defective apoptosis and more specifically to diagnosis and treatment of rheumatoid arthritis. BACKGROUND OF THE INVENTION [0004] Rheumatoid arthritis is a chronic inflammatory arthritis that afflicts approximately 1% of adults (Mitchell, D. 1985. Rheumatoid Arthritis. P D Utsinger, N J Zvaifler, G E Ehrlich, eds. J.B. Lippincott Co., Philadelphia, pp. 133-150). The distribution of affected joints is symmetric and typically involves the small articulations of the hands and feet, although the larger appendicular joints like the shoulders and hips are often affected in established disease. Joint deformities, including ulnar deviation of the metacarpal phalangeal joints of the hand or destruction of the weight bearing joints, can occur late in the disease. [0005] The symptoms of the disease result from a massive increase in the number of cells lining the synovium of the joint. The various cell types which are present include type A synoviocytes, which have the characteristics of monocytes or terminally differentiated macrophages, and type B synoviocytes which are fibroblast-like. As these cells increase in number, the continuous inflammation causes initial symptoms. Eventually, local release of enzymes by the synovial internal lining degrade the extracellular matrix and cause deformity. [0006] The mainstays of therapy for rheumatoid arthritis include non-steroidal anti-inflammatory drugs, injectable gold salts, immunosuppressive agents, and methotrexate. While controlled studies do show some clinical benefit from these drugs, improvement is often limited and toxicity is common. Furthermore, most data suggest that these agents do not halt the rate of cartilage or bone destruction. Hence, a novel treatment that is directed at the pathogenesis of the disease with potential disease modifying activity would be a major improvement. The origin of the cells in the hyperplastic synovial lining in chronic inflammatory joint diseases remains controversial. [0007] Necrosis and apoptosis are two basic processes by which cells may die. In necrosis cell death usually is a result of cell injury. The cells tend to swell and lyse, and the cell contents ultimately spill into the extracellular space. By contrast, apoptosis is a mode of cell death in which single cells are deleted in the midst of living tissues. Apoptosis accounts for most of the programmed cell death (PCD) in tissue remodeling and for the cell loss that accompanies atrophy of adult tissues following withdrawal of endocrine and other growth stimuli. In addition, apoptosis is believed to be responsible for the physiologic death of cells in the course of normal tissue turnover (i.e., tissue homeostasis) (Kerr, J. F.,et al, 1972. Br. J. Cancer 26:239-257; Wyllie, A. H., et al. 1980. Int. Rev. Cytol. 68:251-306). [0008] The effector mechanisms of apoptosis are only incompletely understood, but the nuclear changes which occur appear to be caused by the activation of endogenous calcium-magnesium-sensitive nucleases that cleave chromatin between nucleosomes and reduce the DNA of apoptotic cells. A number of regulators of apoptosis have been identified. Some of these are already familiar as protooncogenes and oncosuppressor genes, including c-myc, bcl-2, p53, and ras. The protooncogene products and oncosuppressor proteins are believed to control cellular susceptibility to apoptosis (Isaacs, J. T. 1994. Curr. Opin. Oncol. 6:82-89). C-myc seems to determine whether cells continuously proliferate or enter apoptosis, depending on the availability of critical growth factors (Bisonnette, R. P., et al. 1994. In Apoptosis II: The Molecular Basis of Apoptosis in Disease. Cold Spring Harbor Laboratory Press). In cultured cells, expansion is usually determined by the presence of c-myc and growth factors, whereas apoptosis is seen when c-myc is present but growth factors are absent. Certain other oncogenes (e.g., ras and bcl-2) rescue cells from susceptibility to apoptosis. The oncosuppressor gene p53 is believed to initiate apoptosis by causing temporary G1/S arrest in cells expressing c-myc (Yonish-Rouach, et al. Mol. Cell Biol. 13:1415-1423.). SUMMARY OF THE INVENTION [0009] The present invention is based on the seminal discovery that a causative agent of cellular accumulation in chronic inflammatory diseases is an insufficient level of apoptosis at the site of cellular accumulation. An insufficient level of apoptosis observed in these accumulation diseases correlates with the presence of altered or mutant polynucleotides or polypeptides known to be involved in apoptosis in the accumulating cells. Taken together, these findings indicate that the insufficient apoptosis among macrophages and fibroblast-like cells which causes the arthritis pathology or among other cells in chronic inflammatory diseases, is due to deficient apoptosis attributable to genes important in regulating apoptosis (e.g., p53). [0010] Therefore, it is desirable to decrease the number of cells which may accumulate in chronic inflammatory disease by enhancing apoptosis at the site of increased cell number. Restoration of apoptosis may be accomplished by providing an apoptosis enhancing amount of functional or wild type gene, such as p53, to the tissues where cellular accumulation is occurring. Alternatively, specific therapy can provide a selective survival advantage only to cells that contain the wild type gene and gene product as opposed to a mutant or altered gene or gene product. [0011] Other features and advantages of the invention will be apparent from the following description of the preferred embodiments thereof, and from the claims. BRIEF DESCRIPTION OF THE DRAWINGS [0012] FIG. 1 shows evidence of apoptosis in the synovium of arthritis patients. A DNA ladder is readily visualized in extracted DNA from 4 synovial tissues. Actinomycin D-treated HL-60 cells are shown as a positive control (RA=ST 1, 3, 4; OA=ST 2). The figure shows a negative image of the ethidium stained gel. [0013] FIG. 2 shows an in situ end labeling (ISEL) assay on frozen sections of rheumatoid arthritis synovial tissue to demonstrate DNA strand breaks. Tissues were counterstained only with eosin, so that normal nuclei are not visualized. Nuclei with fragmented DNA (dark nuclei) are readily distinguished in the intimal lining. [0014] FIG. 3 shows a Western blot experiment demonstrating immunoreactive p53 protein in rheumatoid arthritis fibroblast-like synoviocytes. The demonstration of constitutive p53 expression suggests aberrant p53 regulation. [0015] FIG. 4 shows the distribution of p53 in rheumatoid arthritis synovium using an antibody that detects both wild type and mutant p53 protein (D07, Dako, Inc., Carpenteria, Calif.) and immunohistochemistry. The protein is strongly expressed in the intimal lining. [0016] FIG. 5 shows the distribution of mutant p53 protein using a monoclonal antibody that only detects a mutant form of p53 (monoclonal antibody PAb 240 (Oncogene Sciences, Cambridge, Mass.)). Cells in the synovial lining stain with the mutant-specific antibody and immunohistochemistry (see arrow). [0017] FIGS. 6A and 6B show a combination immunohistochemistry and ISEL. Tissues are counterstained with methyl green to show ISEL-negative nuclei. (Brown color=immunoperoxidase stain for cell surface markers; dark purple color=ISEL-positive nuclei; light turquoise color=methyl green stain of ISEL-negative nuclei) FIG. 6A shows tissue stained with anti-CD68 antibody to detect macrophages. FIG. 6B shows tissue stained with anti-CD45RO antibody to detect memory T-cells. [0018] FIG. 7 shows immunochemistry showing bcl2 expression is prominent in lymphoid aggregates, but is minimal in the intimal lining. [0019] FIG. 8 shows DNA fragmentation in FLS. Cells were treated with various concentrations of act D, anti-fas antibody, IL-1 beta, or TNF-alpha and the extent of DNA fragmentation was determined by tritiated thymidine incorporation. [0020] FIG. 9 shows a histogram from a representative experiment demonstrating fas expression on FLS. The vertical axis shows relative cell number and the horizontal axis shows relative red fluorescence. Cells were stained with either anti-fas antibody or control antibody. DESCRIPTION OF THE PREFERRED EMBODIMENTS Continue reading... Full patent description for Therapy for cellular accumulation in chronic inflammatory diseases Brief Patent Description - Full Patent Description - Patent Application Claims Click on the above for other options relating to this Therapy for cellular accumulation in chronic inflammatory diseases patent application. ###  How KEYWORD MONITOR works... a FREE service from FreshPatents1. Sign up (takes 30 seconds). 2. Fill in the keywords to be monitored. 3. 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