| Tag nucleic acids and probe arrays -> Monitor Keywords |
|
|
  Tag nucleic acids and probe arraysUSPTO Application #: 20080032408Title: Tag nucleic acids and probe arrays Abstract: The invention provides a unique set of nucleic acid sequences which is appropriate for use for a wide variety of applications requiring nucleic acid tags. As such, the sequence tags of the presently claimed invention may be used, for example, to label biological and nonbiological materials, in genotyping applications and in a variety of other analyses. (end of abstract) Agent: Affymetrix, Inc Attn: ChiefIPCounsel, Legal Dept. - Santa Clara, CA, US Inventors: Michael Mittmann, MacDonald Morris, Thomas B. Ryder, David Lockhart USPTO Applicaton #: 20080032408 - Class: 436 56 (USPTO) The Patent Description & Claims data below is from USPTO Patent Application 20080032408. Brief Patent Description - Full Patent Description - Patent Application Claims
PRIORITY CLAIM [0001]This application claims priority of U.S. Provisional Application 60/195,585 filed Apr. 6, 2000 entitled "Tag Nucleic Acids and Probe Arrays", which is incorporated herein by reference for all purposes in its entirety. FIELD OF THE INVENTION [0002]This invention provides sets of nucleic acid tags, arrays of oligonucleotide probes, nucleic acid-tagged sets of recombinant cells and other compositions. The invention relates to the selection and interaction of nucleic acids, and nucleic acids immobilized to solid substrates, including related chemistry, biology, and medical diagnostic uses. BACKGROUND OF THE INVENTION [0003]The use of short nucleic acid sequences as "tags" to identify specific biological substances in a sample is known. For example, tags may be used as a method of or as labels for a wide variety of biological and nonbiological materials, see, for example, Dollinger, The Polymerase Chain Reaction pp. 265-274 Mullis et al., editors (Birkhauser, Boston, 1994) or as a method of screening complex chemical libraries. See, for example, Alper, Science, 264: 1399-1401 (1994); and Needels et al. PNAS 90, 10700-10704 (1993). See also U.S. Pat. Nos. 4,359,353, 4,441,943, 5,451,505 and 5,654,413. [0004]There is great necessity for sets of tag sequences which are known to hybridize effectively to their complementary probe sequences with minimal cross-hybridization between the different tag sequences. The presently claimed invention provides sets of tag sequences, tag sequence kits, and methods of using tag sequences which fulfill these requirements. SUMMARY OF THE INVENTION [0005]The presently claimed invention provides 2050 unique sequences which have been specifically chosen according to strict criteria to produce sequences suitable for a wide variety of "tagging" applications. These sequences are provided as SEQ ID NOs 1-2050. [0006]In one embodiment, some or all of SEQ ID Nos 1-2050 comprise tag sequences. In a further embodiment, some or all of SEQ ID Nos 1-2050 comprise tag-probe sequences. In a further embodiment, the tag-probe sequences are immobilized to a solid support. [0007]The unique sequences of the presently claimed invention may be used alone or in combinations of 10 or more, 100 or more, 200 or more, 500 or more, 1000 or more, 1500 or more, or 2000 or more as nucleic acid tags and/or tag-probes. BRIEF DESCRIPTION OF THE DRAWINGS [0008]FIG. 1 shows a plot of the discrimination score and the signal intensity for 2200 candidate sequences. [0009]FIG. 2 shows an example of the sequences attached to each of the four array features representing a given tag sequence. Four features, organized vertically on the probe array, represent each tag-probe. [0010]FIG. 3 shows the array features from an array designed to probe for the tag sequences of the presently claimed invention. For each of the four tag-probes shown, arranged horizontally across the array, the brightest hybridization signal is seen with the "PM" feature. [0011]FIG. 4 is a scanned image of the hybridization patterns resulting from the hybridization of 2050 different probes containing regions complementary to the SEQ ID Nos 1-2050 to an array comprised of tag-probes corresponding to SEQ ID Nos 1-2050. [0012]FIG. 5 is a scanned image of the hybridization patterns resulting from the hybridization of 50 different probes containing regions complementary to SEQ ID Nos 2001-2050 to an array identical to the array depicted in FIG. 4. [0013]FIG. 6 shows signal intensities from two different independent experiments in which 2000 biotinylated oligonucleotide tags or 50 fluorescein labeled control oligonucleotides were hybridized to arrays designed as described above. [0014]FIG. 7 shows the PM/MM ratios from the data described in FIG. 4 above. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS I. Definitions [0015]As used herein, certain terms may have the following defined meanings. [0016]As used in the specification and claims, the singular forms "a", "an" and "the" include plural references unless the context clearly dictates otherwise. For example, the term "an array" may include a plurality of arrays unless the context clearly dictates otherwise. [0017]An "array" represents an intentionally created collection of molecules which can be prepared either synthetically or biosynthetically. In particular, the term "array" herein means an intentionally created collection of polynucleotides attached to at least a first surface of at least one solid support wherein the identity of each polynucleotide at a given predefined region is known. The terms "array," "biological chip" and "chip" are used interchangeably. Continue reading... Full patent description for Tag nucleic acids and probe arrays Brief Patent Description - Full Patent Description - Patent Application Claims Click on the above for other options relating to this Tag nucleic acids and probe arrays patent application. ###  How KEYWORD MONITOR works... a FREE service from FreshPatents1. Sign up (takes 30 seconds). 2. Fill in the keywords to be monitored. 3. Each week you receive an email with patent applications related to your keywords. Start now! - Receive info on patent apps like Tag nucleic acids and probe arrays or other areas of interest. ### Previous Patent Application: Standard/reference/control for blood coagulation testing Next Patent Application: Fluorescent biomolecule labeling reagents Industry Class: Chemistry: analytical and immunological testing ### FreshPatents.com Support Thank you for viewing the Tag nucleic acids and probe arrays patent info. IP-related news and info Results in 0.89791 seconds Other interesting Feshpatents.com categories: Daimler Chrysler , DirecTV , Exxonmobil Chemical Company , Goodyear , Intel , Kyocera Wireless , |
||
