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Silane mixturesSilane mixtures description/claimsThe Patent Description & Claims data below is from USPTO Patent Application 20070275411, Silane mixtures. Brief Patent Description - Full Patent Description - Patent Application Claims
BACKGROUND OF THE INVENTION [0001]Silanating reagents have been developed which react with and coat surfaces, such as silica surfaces. Monofunctional silanating reagents have been used to form monolayer surface coatings, while di- and tri-functional silanating reagents have been used to form polymerized coatings on silica surfaces, providing reaction sites for covalent attachment of various materials to the surface. Many silanating reagents, however, produce coatings with undesirable properties including instability to hydrolysis and an inadequate ability to mask the silica surface. SUMMARY OF THE INVENTION [0002]The present invention provides silanation compositions that include a mixture of two or more silanation reagents, where at least one silanation reagent includes a functional group capable of supporting polymer synthesis and at least one silanation reagent includes no functional group capable of supporting polymer synthesis. [0003]Silanation reagent compositions (mixtures), one reagent having a functional group capable of supporting polymer synthesis and another silanation reagent having no functional group capable of supporting polymer synthesis, allows for control of the density of polymers on a substrate. An overly high density of polymers on a substrate, e.g., an array of oligonucleotides (probes) on a glass chip, where the array is to be used for determining binding of complementary nucleic acids or "targets," has undesirable properties. Target binding is inhibited by an overly dense probe concentration on the surface of the substrate by steric and electrostatic repulsive forces of overly crowded probes on the surface of the substrate. Where the targets are labeled with fluorescent molecules, for example, inhibition of target binding in turn leads to an undesirable decrease in hybridization signal, which in turn could result in false negatives. [0004]It has been discovered in accordance with the present invention that by silanating with a mixture of functional and non-functional silanating reagents a silane coating is created having increased hydrolytic stability. Merely reducing the density of functional silanes without adding non-functional silanes would result in silane coatings with reduced hydrolytic stability. [0005]In one preferred embodiment, the silanation composition has at least one silanation reagent which includes a functional group capable of supporting polymer synthesis is selected from the group consisting of: [0006]N-(3-(triethoxysilyl)propyl)-4-hydroxybutyramide [0007]N,N-bis(2-hydroxyethyl)-3-aminopropyltriethoxysilane [0008]3-((2-hydroxyethyl)methylamino)propyltrimethoxysilane [0009]N-(2-hydroxyethyl)-N,N-bis(trimethoxysilylpropyl)amine [0010]1,2-bis((2-hydroxyethyl)(3-trimethoxysilylpropyl)aminoethane [0011]3-acetoxypropyltrimethoxysilane [0012](trimethoxysilyloxy)-(C.sub.3-C.sub.12-alkyl)-(triethoxysilane) [0013]3-glycidoxypropyltriethoxysilane and wherein the at least one silane reagent which does not include a functional group capable of supporting polymer synthesis is selected from the group consisting of alkyl-trimethoxysilane, 1,2-bis(trimethoxysilyl)ethane, 1,3-bis(trimethoxysilyl)propane, 1,6-bis(trimethoxysilyl)hexane, 3-(N,N-dimethylamino)propyltrimethoxysilane and N,N-bis(3-trimethoxysilylpropyl)methylamine. [0014]In one preferred embodiment, the silanation reagent including a functional group capable of supporting polymer synthesis is a masked silanation reagent. Many silanes with masked hydroxyl groups, for example, are highly volatile. Volatile, masked silanes can be readily purified by, e.g., distillation, and can be readily employed in gas-phase deposition methods of silanating substrate surfaces. Gas-phase deposition, as opposed to dipping methods provide for more efficient deposition and uniform coating of the substrate. Prior to attaching monomers the masked silanating reagent must be demasked by procedures discussed, infra. [0015]The present invention provides silanation compositions that include a mixture of two or more silanation reagents, where at least one silanation reagent includes a functional group capable of supporting polymer synthesis and at least one silanation reagent includes no functional group capable of supporting polymer synthesis in predetermined ratios. In a preferred embodiment, the ratios are weight to volume ratios (W:V). In another preferred embodiment, the rations are molar ratios. [0016]The present invention provides methods of silanating a surface that comprises contacting a silica or other silicon oxide-containing surface with a silanation composition that includes a mixture of two or more silanation reagent, where at least one silanation reagent includes a functional group capable of supporting polymer synthesis and at least one silanation reagent includes no functional group capable of supporting polymer synthesis. [0017]In a further aspect, the present invention provides a method of preparing an array of polymers that include the steps of providing a substrate, contacting a silica or other silicon oxide-containing surface of the substrate with a silanation composition that includes a mixture of two or more silanation reagents, where at least one silanation reagent includes a functional group capable of supporting polymer synthesis and at least one silanation reagent includes no functional group capable of supporting polymer synthesis; protecting the functional group capable of supporting polymer synthesis with a protecting group or reacting the functional group capable of supporting polymer synthesis with a compound having a reactive group protected by a protecting group; removing the protecting group in selected regions of the surface to provide an exposed functional or reactive group; reacting said exposed group with a monomer, wherein the monomer is coupled to the exposed group; and repeating the steps of removing and reacting to produce the array of polymers. [0018]The present invention also provides for an array of polymers on a surface of a substrate, where the surface of the substrate is silanated by a silanation composition that includes a mixture of two or more silanation reagents, where at least one silanation reagent includes a functional group capable of supporting polymer synthesis and at least one silanation reagent includes no functional group capable of supporting polymer synthesis. BRIEF DESCRIPTION OF THE DRAWINGS [0019]FIG. 1 Depicts two non functional silane compounds (Bis(3-methylamino)propyl)trimethoxysilane (R2) and Bis(trimethoxysilyl)ethane (BTMSE)) and two functional silanes (N-(hydroxyethyl)N-methylamino propyltrimethoxysilane (R2) and N-Hydroxyethyl-N,N-Bis(Trimethoxysilylpropyl)amine). [0020]FIG. 2 Depicts formulations for Silanation of various mixtures of R2+Bisb. Continue reading about Silane mixtures... Full patent description for Silane mixtures Brief Patent Description - Full Patent Description - Patent Application Claims Click on the above for other options relating to this Silane mixtures patent application. 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