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Recombinant dna constructs and methods for controlling gene expression   

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Abstract: The present invention provides molecular constructs and methods for use thereof, including constructs including heterologous miRNA recognition sites, constructs for gene suppression including a gene suppression element embedded within an intron flanked on one or on both sides by non-protein-coding sequence, constructs containing engineered miRNA or miRNA precursors, and constructs for suppression of production of mature microRNA in a cell. Also provided are transgenic plant cells, plants, and seeds containing such constructs, and methods for their use. The invention further provides transgenic plant cells, plants, and seeds containing recombinant DNA for the ligand-controlled expression of a target sequence, which may be endogenous or exogenous. Also disclosed are novel miRNAs and miRNA precursors from crop plants including maize and soy. ...

Agent: Monsanto Company - St. Louis, MO, US
Inventors: Shihshieh Huang, Thomas Malvar, Michael H. Luethy, Philip W. Miller, Larry A. Gilbertson, Edwards Allen, Sara E. Heisel, David K. Kovalic, James K. Roberts, Nancy Houmard, Alessandra Frizz, Yuanji Zhang, Liang Guo, Linda Lutfiyya
USPTO Applicaton #: #20070083952 - Class: 800280000 (USPTO) - 04/12/07 - Class 800 
Related Terms: Intron   
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Related Patent Categories: Multicellular Living Organisms And Unmodified Parts Thereof And Related Processes, Method Of Introducing A Polynucleotide Molecule Into Or Rearrangement Of Genetic Material Within A Plant Or Plant Part, The Polynucleotide Confers Pathogen Or Pest Resistance, Plant Virus Gene Expression From The Polynucleotide
The Patent Description & Claims data below is from USPTO Patent Application 20070083952, Recombinant dna constructs and methods for controlling gene expression.

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