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Nucleotide sequences which code for the rpsl geneRelated Patent Categories: Chemistry: Molecular Biology And Microbiology, Micro-organism, Tissue Cell Culture Or Enzyme Using Process To Synthesize A Desired Chemical Compound Or Composition, Preparing Alpha Or Beta Amino Acid Or Substituted Amino Acid Or Salts Thereof, Lysine; Diaminopimelic Acid; Threonine; ValineNucleotide sequences which code for the rpsl gene description/claimsThe Patent Description & Claims data below is from USPTO Patent Application 20060019357, Nucleotide sequences which code for the rpsl gene. Brief Patent Description - Full Patent Description - Patent Application Claims
BACKGROUND OF THE INVENTION [0001] 1. Field of the Invention [0002] The present invention provides nucleotide sequences from coryneform bacteria which code for the rpsL gene and a process for the fermentative preparation of amino acids using bacteria in which the rpsL gene is enhanced. [0003] 2. Background of the Invention [0004] L-Amino acids, in particular L-lysine, are used in human medicine and in the pharmaceuticals industry, in the foodstuffs industry, and, very particularly, in animal nutrition. [0005] It is known that amino acids are prepared by fermentation from strains of coryneform bacteria, in particular Corynebacterium glutamicum. Because of their great importance, work is constantly being undertaken to improve the preparation processes. Improvements to the process can relate to fermentation measures, such as, for example, stirring and supply of oxygen, or the composition of the nutrient media, such as, for example, the sugar concentration during the fermentation, or the working up to the product form by, for example, ion exchange chromatography, or the intrinsic output properties of the microorganism itself. [0006] Methods of mutagenesis, selection and mutant selection are used to improve the output properties of these microorganisms. Strains which are resistant to antimetabolites or are auxotrophic for metabolites of regulatory importance and produce amino acids are obtained in this manner. [0007] Methods of the recombinant DNA technique have also been employed for some years for improving the strain of Corynebacterium strains which produce L-amino acid, by amplifying individual amino acid biosynthesis genes and investigating the effect on the amino acid production. However, there is a continuing need for new methods of producing L-amino acids. SUMMARY OF THE INVENTION [0008] It is an object of the present invention to provide new methods for an improved fermentative preparation of amino acids. [0009] The present invention is based on the discovery that bacteria in which the rpsL gene is enhanced can be used to fementatively produce amino acids. [0010] Accordingly, the invention provides an isolated polynucleotide from coryneform bacteria, comprising a polynucleotide sequence which codes for the rpsL gene chosen from the group consisting of a [0011] a) polynucleotide which is identical to the extent of at least 70% to a polynucleotide which codes for a polypeptide which comprises the amino acid sequence of SEQ ID No. 2, [0012] b) polynucleotide which codes for a polypeptide which comprises an amino acid sequence which is identical to the extent of at least 70% to the amino acid sequence of SEQ ID No. 2, [0013] c) polynucleotide which is complementary to the polynucleotides of a) or b), and d) polynucleotide comprising at least 15 successive nucleotides of the polynucleotide sequence of a), b) or c), the polypeptide preferably having the activity of the ribosomal protein S12. [0014] The present invention also provides the above-mentioned polynucleotide, this preferably being a DNA which is capable of replication, comprising: [0015] (i) the nucleotide sequence shown in SEQ ID no. 1, or [0016] (ii) at least one sequence which corresponds to sequence (i) within the range of the degeneration of the genetic code, or [0017] (iii) at least one sequence which hybridizes with the sequence complementary to sequence (i) or (ii), and optionally [0018] (iv) sense mutations of neutral function in (i) which do not modify the activity of the protein/polypeptide [0019] In addition, the present invention also provides polynucleotides chosen from the group consisting of [0020] a) polynucleotides comprising at least 15 successive nucleotides chosen from the nucleotide sequence of SEQ ID No. 1 between positions 1 and 499, [0021] b) polynucleotides comprising at least 15 successive nucleotides chosen from the nucleotide sequence of SEQ ID No. 1 between positions 500 and 883, [0022] c) polynucleotides comprising at least 15 successive nucleotides chosen from the nucleotide sequence of SEQ ID No. 1 between positions 884 and 1775. [0023] The present invention also provides [0024] a polynucleotide, in particular DNA, which is capable of replication and comprises the nucleotide sequence as shown in SEQ ID No. 1; [0025] a polynucleotide which codes for a polypeptide which comprises the amino acid sequence as shown in SEQ ID No. 2; [0026] a vector containing the polynucleotide according to the invention, in particular a shuttle vector or plasmid vector, and [0027] coryneform bacteria which contain the vector or in which the rpsL gene is enhanced. [0028] In addition, the present invention also provides the Corynebacterium glutamicum strain DM1545 deposited as DSM 13992 at the Deutsche Sammiung fur Mikroorganismen und Zellkulturen (DSMZ, German Collection of Microorganisms and Cell Cultures, Braunschweig, Germany). [0029] The present invention also provides a process for the fermentative preparation of an L-amino acid, comprising: [0030] a) fermenting coryneform bacteria which produce the L-amino acid and in which at least the rpsL gene or nucleotide sequences which code for it are enhanced, [0031] b) concentrating the L-amino acid in the medium or in the cells of the bacteria, and [0032] c) isolating the L-amino acid. [0033] The present invention also provides a process for discovering RNA, cDNA and DNA in order to isolate nucleic acids or polynucleotides or genes which code for the ribosomal protein S12 or have a high similarity with the sequence of the rpsL gene, which comprises employing the polynucleotide comprising the polynucleotide sequences as claimed in claim 1 as a hybridization probe. [0034] The present invention additionally provides a process for identifying a nucleic acid which codes for the ribosomal protein S12 or have a high similarity with the sequence of the rpsL gene, comprising: [0035] contacting a sample with the polynucleotide sequence as claimed in claim 1 under conditions under hybridization conditions such that the polynucleotide sequence as claimed in claim 1 hybridizes with said nucleic acid when said nucleic acid is present in the sample. [0036] Further, the present invention additionally provides: [0037] a DNA which originates from coryneform bacteria and codes for ribosomal S12 proteins, wherein the associated amino acid sequences between positions 38 to 48 in SEQ ID No. 2 are modified by amino acid exchange; [0038] a DNA which originates from coryneform bacteria and codes for ribosomal S12 proteins, wherein the associated amino acid sequences at position 43 in SEQ ID No. 2 contain any other proteinogenic amino acid excluding L-lysine; and [0039] a DNA which originates from coryneform bacteria and codes for ribosomal S12 proteins, wherein the associated amino acid sequences at position 43 in SEQ ID No. 2 contain L-histidine or L-arginine. [0040] The invention also provides polynucleotides which substantially comprise a polynucleotide sequence, which are obtainable by screening by means of hybridization of a corresponding gene library of a coryneform bacterium, which comprises the complete gene or parts thereof, with a probe which comprises the sequence of the polynucleotide according to the invention according to SEQ ID No. 1 or a fragment thereof, and isolation of the polynucleotide sequence mentioned. [0041] Polynucleotides which comprise the sequences according to the invention are suitable as hybridization probes for RNA, cDNA and DNA, in order to isolate, in the full length, nucleic acids or polynucleotides or genes which code for the ribosomal protein S12 or to isolate those nucleic acids or polynucleotides or genes which have a high similarity with the sequence of the rpsL gene. They can also be applied as a probe on so-called "arrays", micro arrays" or "DNA chips" in order to detect and determine the corresponding polynucleotides or sequences derived therefrom, such as e.g. RNA or cDNA. BRIEF DESCRIPTION OF THE FIGURES [0042] A more complete appreciation of the invention and many of the attendant advantages thereof will be readily obtained as the same becomes better understood by reference to the following Figure in conjunction with the detailed description below. Continue reading about Nucleotide sequences which code for the rpsl gene... Full patent description for Nucleotide sequences which code for the rpsl gene Brief Patent Description - Full Patent Description - Patent Application Claims Click on the above for other options relating to this Nucleotide sequences which code for the rpsl gene patent application. ###  How KEYWORD MONITOR works... a FREE service from FreshPatents1. Sign up (takes 30 seconds). 2. Fill in the keywords to be monitored. 3. Each week you receive an email with patent applications related to your keywords. Start now! - Receive info on patent apps like Nucleotide sequences which code for the rpsl gene or other areas of interest. ### Previous Patent Application: Polynucleotides encoding polypeptides involved in intermediates metabolism of the central metabolic pathway in methylophilus methylotrophus Next Patent Application: Process for the preparation of fexofenadine Industry Class: Chemistry: molecular biology and microbiology ### FreshPatents.com Support Thank you for viewing the Nucleotide sequences which code for the rpsl gene patent info. IP-related news and info Results in 0.13782 seconds Other interesting Feshpatents.com categories: Medical: Surgery , Surgery(2) , Surgery(3) , Drug , Drug(2) , Prosthesis , Dentistry 174 |
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