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07/19/07 - USPTO Class 800 | 30 views | #20070169222 | Prev - Next | About this Page

Nucleic acids encoding anthelmintic agents and plants made therefrom

Title: Nucleic acids encoding anthelmintic agents and plants made therefrom

Related Patent Categories: Multicellular Living Organisms And Unmodified Parts Thereof And Related Processes, Method Of Introducing A Polynucleotide Molecule Into Or Rearrangement Of Genetic Material Within A Plant Or Plant Part, The Polynucleotide Confers Pathogen Or Pest Resistance

Brief Patent Description - Full Patent Description - Patent Claims

The Patent Description & Claims data below is from USPTO Patent Application 20070169222, Nucleic acids encoding anthelmintic agents and plants made therefrom.

1. A method of screening a transgenic plant for anthelmintic activity, comprising contacting a transgenic plant, with a plurality of nematodes under conditions effective to determine whether or not said plant has anthelmintic activity, wherein said transgenic plant contains at least one DNA construct, said construct comprising: a) a nucleic acid encoding a polypeptide effective for catalysing the conversion of a substrate to a C16, C18, or C20 monounsaturated fatty acid product selected from the group consisting of: i) wherein X is hydrogen, CoA, glycerol, a monoglyceride, a diglyceride, ACP, methyl, Na+, phosphatidylcholine, or phosphatidylethanolamine, wherein both R.sub.1 and R.sub.2 are hydroxyl, one of R.sub.1 and R.sub.2 is hydroxyl and the other is hydrogen, or one of R.sub.1 and R.sub.2 is keto and the other is hydrogen, and wherein R.sub.3 is C2, C4, or C6 alkyl; and ii) wherein X is hydrogen, CoA, glycerol, a monoglyceride, a diglyceride, ACP, methyl, Na+, phosphatidylcholine, or phosphatidylethanolamine, and wherein R.sub.3 is C2, C4, or C6 alkyl; and (b) a regulatory element operably linked to said nucleic acid encoding said polypeptide, wherein said regulatory element confers expression in a vegetative tissue of said plant.

2. The method of claim 1, wherein said nematodes are contacted with one or more roots of said transgenic plant.

3. A method of screening a transgenic plant for anthelmintic activity, comprising contacting tissue from a transgenic plant, with a plurality of nematodes under conditions effective to determine whether or not said plant tissue has anthelmintic activity, wherein said transgenic plant contains at least one DNA construct, said construct comprising: a) a nucleic acid encoding a polypeptide effective for catalysing the conversion of a substrate to a C16, C18, or C20 monounsaturated fatty acid product selected from the group consisting of: i) wherein X is hydrogen, CoA, glycerol, a monoglyceride, a diglyceride, ACP, methyl, Na+, phosphatidylcholine, or phosphatidylethanolamine, wherein both R.sub.1 and R.sub.2 are hydroxyl, one of R.sub.1 and R.sub.2 is hydroxyl and the other is hydrogen, or one of R.sub.1 and R.sub.2 is keto and the other is hydrogen, and wherein R.sub.3 is C2, C4, or C6 alkyl; and ii) wherein X is hydrogen, CoA, glycerol, a monoglyceride, a diglyceride, ACP, methyl, Na+, phosphatidylcholine, or phosphatidylethanolamine, and wherein R.sub.3 is C2, C4, or C6 alkyl; and (b) a regulatory element operably linked to said nucleic acid encoding said polypeptide, wherein said regulatory element confers expression in a vegetative tissue of said plant.

4. The method of claim 3, wherein said tissue is root tissue.

Brief Patent Description - Full Patent Description - Patent Claims

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Multicellular living organisms and unmodified parts thereof and related processes

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