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Methods using novel chemiluminescent labelsRelated Patent Categories: Chemistry: Molecular Biology And Microbiology, Measuring Or Testing Process Involving Enzymes Or Micro-organisms; Composition Or Test Strip Therefore; Processes Of Forming Such Composition Or Test Strip, Involving Nucleic AcidMethods using novel chemiluminescent labels description/claimsThe Patent Description & Claims data below is from USPTO Patent Application 20070172878, Methods using novel chemiluminescent labels. Brief Patent Description - Full Patent Description - Patent Application Claims
CROSS-REFERENCE TO RELATED APPLICATION [0001] The present application is a continuation-in-part of applicants co-pending U.S. application Ser. No. 11/079,899 filed on Mar. 14, 2005. FIELD OF THE INVENTION [0002] The present invention relates to a new method of rapidly producing chemiluminescence from electron-rich alkenes by a simple chemical process using inexpensive, readily available reagents. The present invention relates further to chemiluminescent labeling compounds, their use in preparing chemiluminescent labeled compounds and the use of the labeled compounds in assay methods. The invention further relates to assay methods for detecting an analyte and for detecting chemiluminescent-labeled analytes. The methods are useful in immunoassays, nucleic acid probe assays and the like. BACKGROUND OF THE INVENTION [0003] Chemiluminescent detection of analytes has assumed increasing importance in a number of fields, including biomedical analysis, food testing, pathogen identification, forensic investigations and environmental contaminant screening. The means of incorporating a chemiluminescent endpoint into a test or assay can take different forms, such as a chemiluminescent substrate for an enzyme label, a chemiluminescent compound shielded within a structure such as a micelle, liposome or latex particle or by using a chemiluminescent compound as a label. Numerous compounds have been devised for these purposes (R. Handley, H. Akhavan-Tafti, A. P. Schaap, J. Clin. Ligand Assay, 20(4) 302-312 (1997)). The use of chemiluminescent compounds to label species to be detected with small molecules has attracted interest due to the ability to attach multiple labels and to generate the chemiluminescence rapidly. Nevertheless, no single labeling and detection scheme has proven superior in all applications. [0004] Chemiluminescent Labels. Luminol, isoluminol and related cyclic diacyl hydrazides were the first chemiluminescent compounds to be adapted as direct labels by modifying their structure to include a linking substituent. Their use is not satisfactory for many applications due to insufficient light generation limiting detection sensitivity. The low chemiluminescence quantum efficiency, 0.1-1%, and times as long as several minutes for all of the photons to be emitted diminish instantaneous light intensity. [0005] Acridinium esters and acridinium sulfonamides have been used extensively in chemiluminescent immunoassays. (See, e.g., U.S. Pat. Nos. 5,656,500, 5,521,103 and references cited therein). The principal advantages of these labels are the high yield of chemiluminescence (ca. 10%) coupled with the short duration of emission, typically 1-2 sec. Liberating the light energy in such a short flash creates high light intensities. The use of these labels, however, suffers from certain serious drawbacks. Acridinium esters and to a lesser extent the sulfonamides, are prone to hydrolysis to the nonluminescent carboxylic acid, the hydrolysis being accelerated at alkaline pH. The well-known problem of pseudo-base formation from attack of water at the 9 position on the ring requires a separate reaction step to regenerate the acridinium ring. [0006] Ruthenium or osmium-containing complexes produce chemiluminescence when oxidized electrochemically in the presence of a sacrificial amine electron donor. The reaction requires a more costly and complex instrument for performing the electrochemical and light detection steps simultaneously. [0007] U.S. Pat. Nos. 6,017,769 and 6,126,870 disclose a class of acridan compounds with heterosubstituted double bonds and a reactive linking group as chemiluminescent labels. Exemplary compounds contain a vinyl phosphate moiety. No examples of compounds bearing two sulfur substituents on the double bond were disclosed. [0008] U.S. Pat. Nos. 6,858,733 and 6,872,828 disclose acridan ketenedithioacetal compounds as chemiluminescent substrates for peroxidase enzymes. [0009] U.S. Pat. No. 7,186,568 discloses acridan compounds that undergo an electrochemical oxidation at an electrode to produce electrochemiluminescence for use in detection. In one embodiment the acridan compound can have a heterosubstituted double bond and a reactive linking group for use as a chemiluminescent label. Exemplary compounds contain a vinyl phosphate moiety. No examples of compounds bearing two sulfur substituents on the double bond were disclosed. [0010] U.S. Pat. No. 6,046,913 discloses methods for determining an analyte under conditions such that the analyte brings a photosensitizer and a chemiluminescent compound into close proximity. The photosensitizer generates singlet oxygen and activates the chemiluminescent compound when it is in close proximity to produce light. In preferred embodiments the photosensitizer and/or the chemiluminescent compound is associated with a latex particle or oil droplet having a bound specific binding pair member. [0011] While many large molecules are used as labels, including enzymes and the photoprotein aequorin, their use suffers the disadvantage of limiting the number of labels which can be attached to the target species and having the tendency of depositing non specifically on supports and surfaces. [0012] It remains a goal of the assay field to develop chemiluminescent labeling compounds which are small, water soluble molecules, have high chemiluminescence efficiencies, emit the light rapidly upon reaction with simple chemical activating agents, are stable on extended storage and not subject to side reactions. The present invention provides such compounds. [0013] Labeling Procedures. A wide variety of procedures for chemically binding labels to organic and biological molecules are described in the literature (see, for example: L. J. Kricka, Ligand-Binder Assays, Marcel Dekker, Inc., New York, 1985, pp. 15-51 and M. Z. Atassi, "Chemical Modification and Cleavage of Proteins," Chapter 1 in Immunochemistry of Proteins, Vol. 1, Plenum Press, New York, 1977, pp. 1-161, and references therein). Antibodies and proteins are conveniently labeled by reaction of certain nucleophilic groups present in proteins (--SH, --OH, --NH2, --COOH) with chemically reactive groups. Appropriately functionalized nucleic acids and DNA probes can also be labeled by reaction with the corresponding reactive group on a label. Many other types of molecules which can be labeled including antibodies, enzymes, protein antigens, peptides, haptens, steroids, carbohydrates, fatty acids, hormones, nucleosides and nucleotides. [0014] Chemiluminescent Detection in Gels. A method for the detection of the enzyme alkaline phosphatase in a gel using a chemiluminescent substrate has been described (N. Theodosiou, C. Chalot, C. Ziomek, BioTechniques, 13(6), 898-901(1992)). The aforementioned U.S. Pat. Nos. 6,017,769 and 6,126,870 disclose chemiluminescent labeling compounds which can be detected as a label on a compound in an electrophoresis gel. SUMMARY OF THE INVENTION [0015] It is an object of the present invention to provide methods for generating chemiluminescence from a chemiluminescent labeled compound by a simple chemical process using inexpensive, readily available reagents. [0016] It is a further object of the present invention to provide labeling compounds for preparing chemiluminescent labeled compounds. [0017] It is another object of the present invention to provide chemiluminescent labeled compounds. [0018] It is also an object of the present invention to provide labeling compounds of formula I wherein one of the groups R.sup.1-R.sup.11 is a labeling substituent. GENERAL DESCRIPTION [0019] Modern biomedical analyses require the ability to detect very small amounts of compounds due either to low abundance of the analyte in the sample or to limited sample quantity. In addition it must be possible to detect the quantity of the compound precisely over a very wide range of concentrations. Chemiluminescent labeling compounds and methods are disclosed herein which are suitable for these types of analyses. Continue reading about Methods using novel chemiluminescent labels... 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