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Method for biomolecular sensing and system thereofMethod for biomolecular sensing and system thereof description/claimsThe Patent Description & Claims data below is from USPTO Patent Application 20080043222, Method for biomolecular sensing and system thereof. Brief Patent Description - Full Patent Description - Patent Application Claims
[0001] The present application is a continuation of U.S. patent application Ser. No. 10/282,274, filed Oct. 28, 2002, now U.S. Pat. No. 7,______, and claims the benefit of U.S. Provisional Patent Application Ser. No. 60/339,255, filed Oct. 26, 2001, which is hereby incorporated by reference in its entirety. [0002] This invention was supported by the National Science Foundation 9977617, effective date Sep. 1, 1999, and the National Institutes of Health AG18231, effective date Apr. 15, 2000. The U.S. Governnent has certain rights in the invention. FIELD OF THE INVENTION [0003] This invention relates to a method for biomolecular sensing and a system thereof. BACKGROUND OF THE INVENTION [0004] Microarraying and biological sensing are important emerging technologies with huge potential impact on clinical and research medicine. Present methodologies for microarraying and biological sensing are based on fluorescence, radioactive, colorimetry, or surface plasmon resonance assays of molecular recognition chemistry with the former garnering the most attention. [0005] Although these methodologies work, there are problems with each of them. Fluorescence and radioactivity require a special tagging chemistry and thus are time-consuming and cumbersome to use. Additionally, methodologies based on radiation are hard to scale to arrays, and have associated safety and environmental problems. Colorimetry requires chemical amplification when there are large changes in the thickness of the coating and thus is very complicated to adapt to arraying. [0006] The present invention is directed to overcoming these and other deficiencies in the art. SUMMARY OF THE INVENTION [0007] A sensing system in accordance with one embodiment of the present invention includes: a receptor for the at least one target, the receptor including a substrate and translucent coating on the substrate having front and back surfaces; a light source positioned to direct at least a portion of light from the light source toward the coating on the receptor in a manner effective to result in a condition of near perfect interference; and a detector positioned to measure the light reflected from the front and back surfaces of the coating and identifying presence of at least one target based on the measured reflected light. [0008] A method for biomolecular sensing in accordance with another embodiment of the present invention includes: providing a receptor for the at least one target, the receptor including a substrate and a translucent coating on the substrate having front and back surfaces; directing a light at the front and back surfaces of the coating on the receptor in a manner effective to result in a condition of near perfect interference; measuring the light reflected from the front and back surfaces of the coating on the receptor; and providing an output identifying the at least one target based on the measured reflected light. [0009] The present invention provides a system and method for detecting molecular adsorption based on simple reflectivity. One advantage of this reflective technique is that it works without any special tagging chemistry as is required for fluorescence or radioactivity. Additionally, the present invention has a high degree of sensitivity and can determine the precise amount of absorption of a target in a sample. Further, the present invention can be easily adapted to arraying on a large scale and can be done in situ under standard aqueous biological media. The present invention also scales favorably with the size of adsorbate so that it should be extraordinarily sensitive to large systems, such as cells that have been selectively bound using cell membrane markers. BRIEF DESCRIPTION OF THE DRAWINGS [0010] FIG. 1 is a block diagram of a biomolecular sensing system in accordance with one embodiment of the present invention; [0011] FIG. 2 is a side, cross sectional block diagram of a receptor for the biomolecular sensing system in accordance with one embodiment of the present invention; [0012] FIG. 3 is a side, cross sectional block diagram of a receptor for the biomolecular sensing system in accordance with another embodiment of the present invention; [0013] FIG. 4 is a graph of reflectivity versus coating thickness to wavelength ratio; [0014] FIG. 5 is a graph of reflectivity versus wavelength with three different thicknesses of a biomolecule on the substrate in the receptor; [0015] FIG. 6 is a graph of reflectivity versus wavelength for light striking different positions of the receptor; [0016] FIG. 7 is a graph of reflectivity versus angle for several different thicknesses of the absorbed layer; [0017] FIG. 8 is a graph of reflectivity versus wavelength for a surface with a rough oxide coating; [0018] FIG. 9 is a graph of reflectivity versus wavelength for various bandwidth sources; [0019] FIG. 10 is a graph of reflectivity versus wavelength with various angular divergences for the probe source; [0020] FIG. 11 is a three dimensional graph of spots of biotinylated DNA attached to a strepavidin functionalized surface; Continue reading about Method for biomolecular sensing and system thereof... 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