| Method and compositions for identifying anti-hiv therapeutic compounds -> Monitor Keywords |
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Method and compositions for identifying anti-hiv therapeutic compoundsRelated Patent Categories: Chemistry: Molecular Biology And Microbiology, Measuring Or Testing Process Involving Enzymes Or Micro-organisms; Composition Or Test Strip Therefore; Processes Of Forming Such Composition Or Test Strip, Involving Virus Or BacteriophageMethod and compositions for identifying anti-hiv therapeutic compounds description/claimsThe Patent Description & Claims data below is from USPTO Patent Application 20070190523, Method and compositions for identifying anti-hiv therapeutic compounds. Brief Patent Description - Full Patent Description - Patent Application Claims
CROSS-REFERENCE TO RELATED APPLICATION [0001] This application is a Divisional of U.S. patent application Ser. No. 09/994,495, filed Nov. 26, 2001. FIELD OF THE INVENTION [0002] The present invention relates to a sample collection and purification system for biological samples. The present invention also relates to a method for collecting and purifying biological samples. BACKGROUND OF THE INVENTION [0003] Many systems, devices, methods, and processes have been developed for the collection and purification of biological samples. To fully analyze the contents of such collected samples, it is often necessary to purify, archive, and analyze the samples. Such methods include transferring a sample from a collection tray to a purification tray, eluting the sample into an archive tray, and transferring the sample to an analysis tray. Frequently, polymerase chain reaction (hereafter "PCR") is subsequently utilized to amplify nucleic acid components purified from such samples. With each of these process steps comes the potential for cross-contamination between sample collection wells when a multi-well device is used. Other potential problems encountered include cumulative variability introduced during the multiple sample transfer steps, loss or dilution of sample through sample clinging or evaporation, introduction of contaminants, and/or sample misidentification. [0004] Sample collection systems that are known include those described in European Patent Application No. 0 331 127; and in U.S. Pat. Nos. 5,910,246; 4,642,220; 5,665,247; 6,277,648 B1; 6,270,980 B1; 6,153,425; 6,043,080; 5,853,586; 5,955,351; 5,955,271; 5,833,927; 5,783,087; 5,552,325; 5,436,129; 5,356,596; 5,124,041; 5,043,082; 4,990,442; and Re. 35,306. Other known systems include those described in Japanese Patent Publications JP 10-257887, and JP 10-136999. [0005] All of these patents, patent applications, and publications, and all others mentioned herein, are incorporated in their entireties herein, by reference. SUMMARY OF THE INVENTION [0006] The present invention provides a system including a plurality of devices, in each of which a biological sample can be collected, archived, purified, and/or subjected to PCR, transcription, reverse transcription (RT), reverse transcription PCR (RTPCR), or other reaction, without the need to transfer the sample to one or more additional systems or devices. The present invention also provides a system whereby a plurality of such devices can be organized so that respective samples collected in the respective devices can simultaneously be archived, purified, and subsequently analyzed, for example, subjected to PCR, transcription, RT, RTPCR, or another reaction. [0007] According to an embodiment of the present invention, a purification tray system for processing a plurality of fluid samples is provided. This system comprises a plurality of biological sample purification devices. Each device comprises a tubular body having a first end with a first end opening, and a second end with a second end opening. A species-immobilizing filter is secured within the tubular body to collect a target analyte, for example, a nucleic acid molecule or a nucleic acid molecule fragment. The species-immobilizing filter can collect the target analyte through a size-exclusion interaction, a binding interaction, an affinity interaction, or through other filtering mechanisms known to those skilled in the art. Each device further includes a removable cap adapted to seal either the first end opening or the second end opening, and can include two removable caps adapted to respectively seal the first end opening and the second end opening of the device. The purification tray system also includes a tray having a surface adapted to individually seal each of the first end openings or the second end openings of two or more of the plurality of devices. [0008] According to another embodiment of the invention, a combined multiple device and array tray system is provided whereby PCR, RT, RTPCR, or another reaction can be effectively carried out on a plurality of target nucleic acid or nucleic acid fragment samples simultaneously. The target analyte can be bound to or trapped in or on the species-immobilizing filter within each device. Subsequently, the multiple devices arranged in the array tray are subject to conditions that enable PCR amplification. [0009] According to yet another embodiment of the present invention, a sample collection, archiving, purification, and reaction device is provided in the form of a plate system. The plate system includes a plate having a plurality of through-holes with each through-hole having a species-immobilizing filter secured therein. The plate system also includes sealing trays or removable caps for sealing first and second end openings or each through-hole of the plate. A plurality of respective samples can be collected in the respective through-holes of the plate, archived, purified, analyzed, and/or subjected to PCR, transcription, RT, RTPCR, or another manipulation. [0010] Different types of devices can be designed for different specific applications, such as RNA, DNA, or total nucleic acid purification from blood samples, plant or animal cell samples, tissue samples, or microorganism samples. The devices of the present invention can be bar-coded for identifying the type of membrane, type of pre-loaded agent, intended application, and/or for sample identification. [0011] The present invention is further directed to devices that combine the features of sample collection trays, nucleic acid purification trays, archiving trays and PCR and other reaction trays, into a single, universal tray that can be processed on a work station. For example, the multi-purpose system of the present invention can provide a tray capable of automation in robotic work stations such as the Applied Biosystems automated Model 6700 work station. Alternatively, the multi-purpose system of the present invention can provide a tray capable of use in manually operated workstations such as the Applied Biosystems Model 6100 purification work station. [0012] According to a method of the present invention, a biological sample purification device is provided that includes a tubular body having a first end with a first end opening, and a second end with a second end opening, and a species-immobilizing filter secured within the tubular body. The method involves introducing a biological sample into the tubular body through at least one of the first end opening and the second end opening. By causing a pressure differential, for example, through gravity, capillary action, vacuum, or pressurized fluid, the biological sample is moved across the species-immobilizing filter such that a target analyte within the biological sample is immobilized on the species-immobilizing filter. After the species-immobilization step, an additional purification step, and/or a washing step, can be performed to further isolate the target analyte on the filter. Optionally, one or more agents, reagents, or other components can be added prior to sealing. Subsequently, the first end opening and second end opening of the device are sealed, with either removable caps, sealing trays, or a combination thereof, to form a sealed device. The sealed device can subsequently be analyzed or subjected to PCR, transcription, RT, RTPCR, or another reaction to produce a product of the target analyte. If subjected to PCR, transcription, RT, or RTPCR, the product in the sealed device can subsequently be analyzed. [0013] The sealed device, whether containing crude sample or purified sample, and whether or not subjected to a reaction such as PCR, transcription, RT, or RTPCR, can be archived for an extended period of time such as 100 hours or more, and protects the sample sealed therein from evaporation, contamination, and leaking. [0014] The present invention is especially well suited for collecting, archiving, purifying, PCR, transcription, RT, or RTPCR processing, and analyzing samples such as blood samples and other nucleic acid-containing samples. [0015] In another embodiment of the invention a method is provided for the collection of biological samples in the sealable devices disclosed herein. In another embodiment, the present invention provides a method for purifying a biological sample collected in the device. In yet another embodiment of the invention, a method is provided for archiving biological samples. [0016] The embodiments of the present invention provide several advantages over prior sample collection, archiving, purification, and PCR systems. The present invention eliminates the need to transfer samples from a collection vessel to a purification tray. The present invention also reduces the potential for sample mis-identification associated with transfer steps. Another advantage of the present invention is the reduction in purification time by the elimination of post-purification sample elution, dilution, and reaction tray transfer steps. The present invention also reduces the potential for cross-contamination among multiple sample containment devices. In addition, the present invention eliminates the need to use additional reagents, such as sample elution and dilution solutions, and the need to load such additional reagents into collection, purification, and reaction instruments, assemblies, or devices. BRIEF DESCRIPTION OF THE DRAWINGS [0017] The present invention will become more fully understood from the detailed description given herein and the accompanying drawings. The accompanying drawings and detailed description of the present invention set forth herein are illustrative only and are not intended to limit the scope of the present invention defined by the appended claims. In the accompanying drawings: [0018] FIG. 1 is a perspective, exploded, side view in partial phantom of a capsular device including removable and sealable end caps, according to an embodiment of the present invention; [0019] FIG. 2 is a perspective view of a system according to an embodiment of the present invention including an array tray having through-holes and a plurality of capsular devices each partially inserted in a respective through-hole of the array tray; Continue reading about Method and compositions for identifying anti-hiv therapeutic compounds... Full patent description for Method and compositions for identifying anti-hiv therapeutic compounds Brief Patent Description - Full Patent Description - Patent Application Claims Click on the above for other options relating to this Method and compositions for identifying anti-hiv therapeutic compounds patent application. ###  How KEYWORD MONITOR works... a FREE service from FreshPatents1. Sign up (takes 30 seconds). 2. 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