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Medicinal preparation containing phenylethanoid glycosides extracted from herbaceous plant, cistanche tubulosa (schenk.) wight, process of making the same, and uses of the sameRelated Patent Categories: Drug, Bio-affecting And Body Treating Compositions, Plant Material Or Plant Extract Of Undetermined Constitution As Active Ingredient (e.g., Herbal Remedy, Herbal Extract, Powder, Oil, Etc.)Medicinal preparation containing phenylethanoid glycosides extracted from herbaceous plant, cistanche tubulosa (schenk.) wight, process of making the same, and uses of the same description/claimsThe Patent Description & Claims data below is from USPTO Patent Application 20060233896, Medicinal preparation containing phenylethanoid glycosides extracted from herbaceous plant, cistanche tubulosa (schenk.) wight, process of making the same, and uses of the same. Brief Patent Description - Full Patent Description - Patent Application Claims
FIELD OF THE INVENTION [0001] The present invention relates generally to a medicinal preparation derived from a herbaceous plant, and more particularly to a medicinal preparation containing phenylethanoid glycosides which are extracted from a herbaceous plant belonging to the genus Cistanche. The preparation is used as an active ingredient of the drug capable of prevention of senile dementia and inhibition of aggregation of blood platelets. The present invention also covers a process for making the medicinal preparation as well as the uses of the medicinal preparation. BACKGROUND OF THE INVENTION [0002] The senior citizens are generally vulnerable to various physical and mental impairments for which there is often no panacean remedy. The senile dementia is a case in point. However, it is clinically evident that the fleshy stems of herbs belonging to the genus Cistanche are effective in treatment of infertility, impotency, constipation, etc. In addition, the preparation made from the fleshy stems of such perennial herbs as described above is nourishing to blood and kidney. These parasitic and perennial herbs are widely cultivated in the northwestern provinces of China and are locally known as "desert ginseng". The most abundantly cultivated species of the genus Cistanche is Cistanche tubulosa (Schenk.) Wight. [0003] According to the systematic research done by the Japanese scientists on the chemical constituents and the pharmacological activities of the perennial herbs (genus Cistanche), phenylethanoid glycosides are the principal active ingredients of these perennial herbs. Such active ingredients are effective antioxidants, metabolic promoters, memory enhancers, sexuality enhancers, etc. The medicinal properties of various phenylethanoid glycoside compounds have been studied by many researchers. For such information, please refer to the following publications: Sato T., et al. Yakugaku Zasshi, 1985, 105 (12): 1131; Jimenez C., et al. Nat Prod Rep, 1994, 11 (6): 591; Cometa F., et al. Fitoterapia, 1993, 64 (3): 195. SUMMARY OF THE INVENTION [0004] These inventors of the present invention have had more than ten years of research experiences on the perennial herbs of the genus Cistanche. Among all species of the genus Cistanche, one species, Cistanche tubulosa (Schenk.) Wight, contains the greatest amount of phenylethanoid glycoside compounds. The inventors of the present invention introduce a novel process for extracting the phenylethanoid glycosides from Cistanche tubulosa (Schenk.) Wight, as well as a medicinal preparation containing phenylethanoid glycosides. As a result of a number of pharmacological tests conducted by these inventors of the present invention, the medicinal preparation was found to have a significant effect on memory enhancement, thrombosis prevention, inhibition of blood platelets aggregation, etc. [0005] The primary objective of the present invention is to provide a medicinal preparation containing phenylethanoid glycosides which are extracted from a herbaceous plant, Cistanche tubulosa (Schenk.) Wight. It is another objective of the present invention to provide a process for making the medicinal preparation containing phenylethanoid glycosides. It is still another objective of the present invention to provide a medicinal composition for prevention of senile dementia, and a medicinal composition for prevention of aggregation of blood platelets. [0006] The medicinal preparation of the present invention contains 10-70% of echinacoside by weight of the preparation, and 1-40% of acteoside by weight of the preparation. [0007] Preferably, the preparation of the present invention contains 25-70% of echinacoside by weight of the preparation, and 5-40% of acteoside by weight of the preparation. [0008] Preferably, the medicinal preparation of the present invention is made from the fleshy stems of Cistanche tubulosa (Schenk.) Wight. [0009] Preferably, the medicinal preparation of the present invention further contains 2'-acetylacteoside; campneoside I; campneoside II; cistantubuloside A, B.sub.1, B.sub.2, C.sub.1, C.sub.2; crenatoside; decaffeoylacteoside; isoacteoside; rhodioloside; syringalide A; 3'-.alpha.-L-rhamnopyranoside, and tubuloside A, with each in an amount less than 5% by weight of the medicinal preparation. [0010] The process of the present invention for making the medicinal preparation involves a first step in which the subterranean portions of Cistanche tubulosa (Schenk.) Wight are extracted by a first polar solvent. The extract so obtained is then introduced into a column which is packed with hydrophobic macro-porous polymeric beads, thereby enabling phenylethanoid glycosides to be adsorbed on the polymeric beads. The relatively less strongly adsorbed compounds are then eluted from the column by use of a second polar solvent serving as a mobile phase, with most of phenylethanoid glycosides still being adsorbed on the polymeric beads. Finally, phenylethanoid glycosides are eluted from the column by use of a third polar solvent so as to obtain an eluate which contains phenylethanoid glycosides. The third polar solvent is lower in polarity than the second polar solvent. [0011] Preferably, the subterranean portions of Cistanche tubulosa (Schenk.) Wight are fleshy stems. The subterranean portions which are mixed with the first polar solvent, and the resulting mixture is then cooked to boil for a period lasting 0.5-10 hours. The mixture is subsequently filtered so as to obtain a solution. The solution is an extract, which may be in a concentrated form by decompression. Preferably, the mixture contains the subterranean portions of Cistanche tubulosa (Schenk.) Wight and the first polar solvent in a weight ratio ranging from 1:4 to 1:20. Preferably, the first polar solvent is either water, or a mixed solvent containing water and ethyl alcohol. Preferably, the polymeric bead of the column used in the process of the present invention is a cross-linked polyaromatics. More preferably, the polymeric bead is formed of cross-linked polystyrene, or a cross-linked copolymer of styrene and divinyl benzene. Preferably, the second polar solvent is water. Preferably, the third polar solvent is methanol, ethanol, a water-methanol mixture, or a water-ethanol mixture. More preferably, the third polar solvent is a mixture of water and ethanol. [0012] The process of the present invention further comprises removing the solvent that is contained in the eluate so produced, thereby resulting in production of a dry preparation. [0013] The preparation of the present invention can be used to make a medicinal composition for prevention of senile dementia. The medicinal composition contains a therapeutically effective amount of the preparation as an active ingredient and a pharmaceutically acceptable carrier or diluent for the active ingredient. [0014] The preparation of the present invention can also be used to make a medicinal composition for inhibition of blood platelets aggregation. The medicinal composition contains a therapeutically effective amount of the preparation and a medicinally-allowable carrier or diluent. The preparation is used as an active ingredient of the compound. [0015] The present invention also discloses a method of treating and preventing an individual suffering senile dementia comprising administering to the individual a therapeutically effective amount of the preparation of the present invention. [0016] The present invention further discloses a method of inhibiting blood platelets aggregation in an individual comprising administering to the individual a therapeutically effective amount of the preparation of the present invention. DETAILED DESCRIPTION OF THE INVENTION [0017] The present invention provides a preparation containing phenylethanoid glycoside compounds from Cistanche tubulosa (Schenk.) Wight, which contains 10-70% of echinacoside by weight of the preparation, and 1-40% of acteoside by weight of the preparation. The phenylethanoid glycoside compounds derived from Cistanche tubulosa (Schenk.) Wight have the following chemical structure: [0018] and include 2'-acetylacteoside; campneoside I; campneoside II; cistantubuloside A, B.sub.1, B.sub.2, C.sub.1, C.sub.2; crenatoside; decaffeoylacteoside; isoacteoside; rhodioloside; syringalide A; 3'-.alpha.-L-rhamnopyranoside, and tubuloside A shown in Table 1, in which most of the compounds are in a minute or trace amount in the preparation except echinacoside and acteoside. TABLE-US-00001 TABLE 1 principal ingredients of the preparation Ingredient R.sub.1 R.sub.2 R.sub.3 R.sub.4 R.sub.5 R.sub.6 R.sub.7 2'-Acetylacteoside Ac Rha Cf H H OH OH Acteoside H Rha Cf H H OH OH Campneoside I H Rha Cf H OMe(S/R) OH OH Campneoside II H Rha Cf H OH(S/R) OH OH *Cistantubuloside A H Rha Cf Glc H H OH *Cistantubulosides B.sub.1/B.sub.2 H Rha Cm/c-Cm Glc H OH OH *Cistantubulosides C.sub.1/C.sub.2 H Rha Cf Glc OH(S/R) OH OH Decaffeoylacteoside H Rha H H H OH OH Echinacoside H Rha Cf Glc H OH OH Isoacteoside H Rha H Cf H OH OH Rhodioloside H H H H H H OH (Salidroside) Syringalide A 3'-.alpha.-L-rhamnopyranoside H Rha Cf H H H OH Tubuloside A Ac Rha Cf Glc H OH OH Crenatoside See following structure *new compound Ac: Acetyl Cf: trans-Caffeoyl Cm: trans-Coumaroyl c-Cm: cis-Coumaroyl Glc: .beta.-D-Glucopyranose Rha: .alpha.-L-Rhamnopyranose crenatoside [0019] All the compounds listed in Table 1 were verified by means of a high performance liquid chromatography, which was obtained under the following conditions: stationary phase being silicone of C18 alkyl silane; mobile phase being acetonitrile-0.05M phosphoric acid aqueous solution (gradient eluting (4:96.fwdarw.15:85)), flow rate of 1 ml/min, and detecting wavelength of 330 nm. [0020] The process of making the medicinal preparation of the present invention is explicitly described hereinafter. The process of the present invention involves two steps, which are extraction and purification. In the first step, the fleshy stems of Cistanche tubulosa (Schenk.) Wight are cut into flakes or are comminuted into fine particles or powder. The flakes or fine particles are then soaked in a medium which is water, ethanol, methanol, or low fatty alcohol. The extraction is carried out at room temperature. The filtrate is concentrated in vacuo, thereby resulting in formation of an extract. The purification of the extract is done by heating the extract in water before the extract is transferred to an adsorption column packed with macro-porous adsorption resin of D-101 type or AB-8 type. The column is eluted with water, methanol, ethanol, a water-containing methanol, or a water-containing ethanol, etc. The eluting may be carried out with a solution of a constant concentration or solutions of gradient concentrations. The eluate is collected, concentrated and then dried by a conventional drying method. Upon completion of the drying of the eluate, the medicinal preparation of the present invention is obtained. The medicinal preparation so produced contains phenylethanoid glycosides and is used for making medicinal compositions for use in prevention of senile dementia, or in inhibition of aggregation of blood platelets. Continue reading about Medicinal preparation containing phenylethanoid glycosides extracted from herbaceous plant, cistanche tubulosa (schenk.) wight, process of making the same, and uses of the same... 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