| Fluid processing device for oligonucleotide synthesis and analysis -> Monitor Keywords |
|
|
 
Fluid processing device for oligonucleotide synthesis and analysisRelated Patent Categories: Chemical Apparatus And Process Disinfecting, Deodorizing, Preserving, Or Sterilizing, Chemical Reactor, Bench ScaleFluid processing device for oligonucleotide synthesis and analysis description/claimsThe Patent Description & Claims data below is from USPTO Patent Application 20060153745, Fluid processing device for oligonucleotide synthesis and analysis. Brief Patent Description - Full Patent Description - Patent Application Claims
FIELD [0001] The present teachings relate to fluid processing devices and methods for using the same. BACKGROUND [0002] One of the challenges encountered in fluid processing devices, particularly devices designed for high throughput operations, is how to effectively control fluid flow. It is especially difficult to individually and independently control fluid flow in thousands of micro-channels without resorting to the fabrication of sophisticated valving systems which can make microfluidic devices very expensive. A device and method for controlling fluid flow in a microfluidic system is desirable. SUMMARY [0003] According to various embodiments, a fluid processing device is provided that includes a plurality of reaction sites, a first manifold in fluid communication with each of the reaction sites, a second manifold in fluid communication with each of the reaction sites, and at least one surface tension control valve positioned in at least one channel between the first manifold and at least one of the reaction sites. The reaction sites can each comprise support structures, for example beads, or an inner surface, suitable for the attachment of oligomers or oligomer precursors thereto. According to various embodiments, the fluid processing device can comprise a plurality of surface tension control valves each in fluid communication with the first manifold and one or more of the reaction sites. [0004] According to various embodiments, the fluid processing device can comprise reactants and/or reaction components capable of producing an oligomer in at least one of the reaction sites, or a system that includes sources of reactants and/or reaction components. [0005] According to various embodiments, a system is provided that can comprise a fluid processing device as described herein, and an electromagnetic radiation source capable of emitting electromagnetic radiation and directing the radiation toward one or more surface tension control valves in the device. Alternatively or additionally, the system can comprise other valve-actuating devices besides an electromagnetic radiation source. Exemplary actuators can comprise heaters adapted to direct heat toward one or more surface tension control valves, or an electrical source adapted to supply an electrical signal to one or more surface tension control valves. By controlling the one or more surface tension control valves, the systems described herein can be used in directing the flow of reaction components in an order useful for carrying out an oligonucleotide synthesis reaction within one or more of the plurality of reaction sites. [0006] According to various embodiments, a system is provided that comprises an electromagnetic radiation source or other actuating source, a reflective device, a pump, and a thermocycler. The system can be adapted so that the reflective device can direct electromagnetic radiation emitted from the electromagnetic radiation source toward the one or more surface tension control valves to selectively open or close the respective one or more surface tension control valves. The pump can be adapted to add or remove materials from the channels and reaction sites. The thermocycler can be adapted to control the temperature of the reaction sites, for example, to promote an isothermal or thermally cycled nucleic acid sequence amplification and/or detection assay. The system can comprise one or more control units to control the actuating source, to control the reflective device, to control the pump, and/or to control the thermocycler. [0007] According to various embodiments, a method is provided for synthesizing oligonucleotides or other chemical structures, from component building blocks. The method can comprise, for example, introducing a first monomer into a first fluid distribution manifold of a fluid processing device; opening at least one surface tension control valve in fluid communication with both the first fluid distribution manifold and at least one respective reaction site, to form an open surface tension control valve; moving the first monomer from the first manifold, through the at least one open surface tension control valve, and into the at least one respective reaction site; and attaching the first monomer to a first structure in the at least one respective reaction site to form an extended structure. The first monomer can be, for example, a nucleotide, a nucleotide base, a nucleotide analog, a protected chemical building block, or another monomeric building block, unit, or structure that can bond with and extend off of a support or precursor structure. The first monomer can be a protected first monomer, the extended structure can be a protected extended structure, and the method can further comprise: washing the at least one respective reaction site subsequent to the attaching; closing the at least one surface tension control valve; introducing a deprotecting agent into the first manifold; opening the at least one surface tension control valve to form at least one reopened surface tension control valve; moving the deprotecting agent from the first manifold, through the at least one reopened surface tension control valve, and into the at least one respective reaction site; and deprotecting the extended protected structure to form a deprotected extended structure. An additional monomer can then be added to the deprotected extended structure and the process can be repeated. BRIEF DESCRIPTION OF THE DRAWINGS [0008] Various embodiments of the present teachings are exemplified by the accompanying drawings. The teachings are not limited to the embodiments depicted, and include equivalent structures and methods as set forth in the following description and as would be known or recognized by those of ordinary skill in the art given the present teachings. In the drawings: [0009] FIG. 1 is a cross-sectional view through a portion of a device according to various embodiments and including a surface tension control valve; [0010] FIG. 2A is a cross-sectional view through a portion of a device according to various embodiments and including a surface tension control valve in a closed state; [0011] FIG. 2B is the same cross-sectional view shown in FIG. 2A, but after the surface tension control valve has been opened; [0012] FIG. 3A is a side view of a surface tension control valve showing in phantom the effect that actuation of a surface tension control device has on the shape of a drop of water; [0013] FIG. 3B is a side view of a surface tension control device showing in phantom the effect that actuation of the device has on the shape of a drop of water; [0014] FIG. 3C is a partial cross-sectional view of a surface tension control device showing the direction of movement of a drop of water, resulting from actuation of the valve; [0015] FIG. 4A is a cross-sectional view through a portion of a device according to various embodiments and showing a light-activated surface tension control valve in a closed state; [0016] FIG. 4B is the same cross-sectional view as shown in FIG. 4A, but wherein the light-activated surface tension control valve is in an open state; [0017] FIG. 4C is the same cross-sectional view as shown in FIG. 4A, but wherein the light-activated surface tension control valve is in an open state and liquid has passed through the valve; [0018] FIG. 4D is the same cross-sectional view as shown in FIG. 4A, but wherein the valve is in a closed state after liquid has passed through the valve; [0019] FIG. 5 is a top plan view of a portion of a fluid processing device according to various embodiments; [0020] FIG. 6 is a top plan close-up view of a portion of a fluid processing device according to various embodiments; Continue reading about Fluid processing device for oligonucleotide synthesis and analysis... Full patent description for Fluid processing device for oligonucleotide synthesis and analysis Brief Patent Description - Full Patent Description - Patent Application Claims Click on the above for other options relating to this Fluid processing device for oligonucleotide synthesis and analysis patent application. ###  How KEYWORD MONITOR works... a FREE service from FreshPatents1. Sign up (takes 30 seconds). 2. Fill in the keywords to be monitored. 3. Each week you receive an email with patent applications related to your keywords. Start now! - Receive info on patent apps like Fluid processing device for oligonucleotide synthesis and analysis or other areas of interest. ### Previous Patent Application: Apparatus for dispersing scents into the environment Next Patent Application: Mat for mounting a pollution control element in a pollution control device for the treatment of exhaust gas Industry Class: Chemical apparatus and process disinfecting, deodorizing, preserving, or sterilizing ### FreshPatents.com Support Thank you for viewing the Fluid processing device for oligonucleotide synthesis and analysis patent info. IP-related news and info Results in 0.26699 seconds Other interesting Feshpatents.com categories: Novartis , Pfizer , Philips , Polaroid , Procter & Gamble , 174 |
 * Protect your Inventions * US Patent Office filing 
PATENT INFO |
|
