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06/25/09 - USPTO Class 435 | 1 views | #20090162866 | Prev - Next | About this Page

Compositions and methods for obtaining nucleic acids from sputum

Title: Compositions and methods for obtaining nucleic acids from sputum

Brief Patent Description - Full Patent Description - Patent Claims

The Patent Description & Claims data below is from USPTO Patent Application 20090162866, Compositions and methods for obtaining nucleic acids from sputum.

What is claimed:

1. A device for storage of nucleic acid from a biological sample, said device comprising: (a) a container having a barrier capable of disestablishment, said barrier separating a reagent compartment from a sample receiving compartment; and (b) a nucleic acid storage composition within said reagent compartment, wherein upon disestablishment of said barrier there is fluid communication between said sample receiving compartment and said reagent compartment to facilitate mixing of said biological sample with said nucleic acid storage composition.

2. The device according to claim 1, which additionally comprises means for closing said container, means for disestablishment of the barrier, or both.

3. The device according to claim 1, wherein the storage composition comprises a denaturing agent, a chelator and has a pH of greater than 5.0.

4. The device according to claim 1, which additionally comprises a protease dried on an interior surface of the device.

5. The device according to claim 4, wherein the protease is proteinase K.

6. The device according to claim 1, wherein the nucleic acid is DNA.

7. The device according to claim 1, wherein the nucleic acid is from a virus, a bacterium or a mammalian subject.

8. The device according to claim 1, wherein the biological sample is tissue or a bodily fluid.

9. The device according to claim 8, wherein the bodily fluid is sputum.

10. The device according to claim 2, wherein the displaceable barrier is a pivoting disk.

11. The device of claim 2, wherein the means for closing said container includes means for disestablishment of said barrier.

12. The device of claim 2, wherein the means for closing said container is an airtight lid.

13. A method of preserving a nucleic acid from a biological sample comprising: (a) placing a nucleic acid storage composition into a reagent compartment of a storage container; (b) placing a biological sample into a sample receiving compartment of said storage device, said sample receiving compartment separated from said reagent compartment by a barrier; (c) closing said storage container; and (d) disestablishing said barrier such that said composition contacts said biological sample to form a mixture thereby preserving the nucleic acid from said biological sample.

14. The method according to claim 13, wherein the storage composition comprises a denaturing agent, a chelator and has a pH of greater than 5.0.

15. The method according to claim 14, additionally comprising the step of storing said mixture for at least one day at room temperature.

16. The method according to claim 14, additionally comprising the step of adding a protease to the nucleic acid containing mixture.

17. The method according to claim 16, wherein the protease is proteinase K.

18. The method according to claim 13, wherein the nucleic acid is DNA.

19. The method according to claim 13, wherein the nucleic acid is from a virus, a bacterium or a mammalian subject.

20. The method according to claim 13, wherein the biological sample is tissue or a bodily fluid.

21. The method according to claim 20, wherein the bodily fluid is sputum.

22. The method of claim 13, further comprising recovery of said nucleic acid from said mixture.

23. The method of claim 13, wherein the closing of said container is coupled to the disestablishing of said barrier.

24. A sample receiving container comprising: (a) a sample receiving compartment; (b) a reagent storage compartment; (c) a barrier separating said sample receiving compartment from said reagent storage compartment and capable of disestablishment; and (d) means for closing said container that comprises means for disestablishing said barrier to allow fluid communication between said sample receiving compartment and said reagent storage compartment.

25. The container of claim 24, which additionally comprises a nucleic acid storage composition within said reagent storage compartment.

26. The container of claim 25, wherein the storage composition comprises a denaturing agent, a chelator and has a pH of greater than 5.0.

27. The container of claim 26, which additionally comprises a protease dried on an interior surface of said container.

28. The container of claim 27, wherein said protease is proteinase K.

29. The container of claim 24, wherein said barrier is a disc pivotable about a pivot axis from a closed position to an open position, such that when said barrier is in the open position there is fluid communication between sample receiving compartment from said reagent storage compartment.

Brief Patent Description - Full Patent Description - Patent Claims

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