Chemistry: molecular biology and microbiology inventions 01/08

20080026362 - Three-dimensional culture containing human articular chondrocytes with induced terminal differentiation changes, and preparation process and uses of the same: Disclosed herein is a three-dimensional culture containing human articular chondrocytes with induced terminal differentiation changes, as well as a process for preparing the same. The three-dimensional culture, which was found to mimic the biological characteristics of articular chondrocytes that undergo terminal differentiation in vivo, can be used as a tool... Agent: Townsend And Townsend And Crew, LLP

20080026364 - Identification of leucocytes bearing diagnostic markers for transmissible spongiform encephalopathies: The invention provides a simple method for diagnosing a non-symptomatic or symptomatic human or animal with transmissible spongiform encephalopathy.... Agent: Mcdonnell Boehnen Hulbert & Berghoff LLP

20080026373 - Assays based on light emission from analyte complexes within a cassette: Assays based on probes attached to surfaces enclosed within cassettes and cassettes and reading stations for the assays. After liquids flow over the probe or probe array to form an array of photo-emissive analyte complexes, and prior to reading, (a) liquid is removed by flow, and (b) a drying gas... Agent: Fish & Richardson PC

20080026369 - Chemical analysis using dynamic viscometry: The invention relates to a method for determining the reactive state of a chemical reaction process in a reaction mixture (110), in particular an amplification reaction for nucleic acids. Said method comprises a viscosity determination, which preferably uses a dynamic viscometer (1). The invention also relates to an improved dynamic... Agent: Sughrue Mion, PLLC

20080026383 - Chronic lymphocytic leukaemia: The invention relates to materials and methods for evaluating the prognosis of a patient presenting with chronic myelogenous leukaemia (CLL) and for CLL therapy.... Agent: Marshall, Gerstein & Borun LLP

20080026374 - Compositions and methods for isolation of biological molecules: The present invention provides charged separation media, kits comprising the separation media, and methods of using the same to bind and optionally isolate biological molecules. The charged separation media and methods utilize a polyion non-covalently bound to the separation media to bind the desired biological molecule to the separation media.... Agent: Senniger Powers (sgm)

20080026375 - Compositions and methods for isolation of biological molecules: The present invention provides charged separation media, kits comprising the separation media, and methods of using the same to bind and optionally isolate biological molecules. The charged separation media and methods utilize a polyion non-covalently bound to the separation media to bind the desired biological molecule to the separation media.... Agent: Senniger Powers (sgm)

20080026382 - Enzymatic labeling of rna: Methods are described in which a sample containing RNA is contacted with an enzyme having an RNA ligation activity in the presence of a labeled substrate to provide labeled RNA. Methods of performing an array analysis of a labeled RNA sample are also described.... Agent: Agilent Technologies Inc.

20080026376 - Kex2 cleavage regions of recombinant fusion proteins: The invention relates to a fusion DNA construct comprising a KEX2 region comprising a KEX2 site and a KEX2 site pre-sequence immediately 5′ to the KEX2 site, a fusion polypeptide, vectors and cells comprising the fusion DNA construct, methods for producing desired proteins from filamentous fungal cells and methods for... Agent: Lynn Marcus-wyner Genecor International, Inc.

20080026370 - Method for geno-and pathotyping pseudomonas aeruginosa: The invention relates to a method for genotyping and pathotyping bacteria of the species Pseudomonas aeruginosa by means of hybridization assays on a biochip or microarray. The invention further relates to specific oligonucleotide probes, which can be employed within the scope of the detection method, as well as to biochips... Agent: Steptoe & Johnson LLP

20080026368 - Method for the specific rapid detection of beverage-spoiling microorganisms: The invention relates to a method for the specific rapid detection of beverage-spoiling micro-organisms by means of in situ hybridisation. The invention also relates to specific oligonucleotide probes that are used in the detection method, and to kits containing said oligonucleotide probes.... Agent: Knobbe Martens Olson & Bear LLP

20080026379 - Nucleotide analogs: The invention provides nucleotide analogs for use in sequencing nucleic acid molecules.... Agent: Cooley Godward Kronish LLP Attn: Patent Group

20080026380 - Nucleotide analogs: The invention provides nucleotide analogs for use in sequencing nucleic acid molecules.... Agent: Cooley Godward Kronish LLP Attn: Patent Group

20080026381 - Nucleotide analogs: The invention provides nucleotide analogs for use in sequencing nucleic acid molecules.... Agent: Cooley Godward Kronish LLP Attn: Patent Group

20080026378 - Prediction and prophylactic treatment of type 1 diabetes: An in vitro method for predicting the onset of type 1 diabetes (T1D) in a subject, comprises the steps of: (a) measuring the concentration of at least one amino acid, amino acid derivative or amino acid metabolite in a biological sample taken from the subject; (b) determining the subject's HLA... Agent: Dinsmore & Shohl LLP

20080026377 - Skin wrinkle application of genomic replacement therapy: Genomic Replacement Therapy uses the human genome found in mantle dentin of a patient's tooth to affect other cells of the body. Mantle Dentin cells in the developed tooth do not reproduce, therefore the DNA is “younger”. Replacing the genome with a “younger” one will hopefully return youthful appearances to... Agent: John M. Pavelchik

20080026405 - Biomarkers of mild cognitive impairment and alzheimer's disease: A method for quantifying a neurodegenerative disorder in a patient that includes obtaining a fluid sample from the subject; measuring a protein biomarker complex in said fluid sample and correlating the measurement with mild cognitive impairment or Alzheimer's disease status. The biomarkers include those that comprise at least one of... Agent: Stites & Harbison PLLC

20080026411 - Diagnostic and prognostic methods and compositions of matter for cell proliferative diseases: A method for diagnosing a cell proliferative disease expressing DcR3/TR6 in a patient, which comprises the step of measuring the concentration of DcR3/TR6 in the patient's blood, plasma or serum sample, wherein a concentration of DcR3/TR6 higher than that present in the serum of a patient not suffering of a... Agent: Goudreau Gage Dubuc

20080026414 - Method for the determination of the formation of endothelins for medical diagnostic purposes, and antibodies and kits for carrying out such a method: The invention relates to an in vitro method for detecting the formation of endothelins during serious illnesses, especially cardiovascular diseases, inflammations, sepsis and cancer, in whole blood, plasma or serum of a human patient for medical diagnosis. Using this method, relatively long-lasting peptide fragments, especially a C-terminal peptide fragment, of... Agent: Heslin Rothenberg Farley & Mesiti PC

20080026416 - Model taste cells and methods of use for identifying modulators of taste sensation: The present invention provides model taste cells that naturally or recombinantly express taste receptors and relevant cellular proteins and/or molecules useful for taste signal transduction. The present invention further provides methods of use for these model taste cells for screening for compounds that modulate sweet and/or other taste signal transduction.... Agent: Sutherland Asbill & Brennan LLP

20080026419 - Method and systems for tissue culture: Systems and methods for providing dynamic mechanical loading and mechanical property assessment of tissue cultures in open dish cultures are provided. An actuator may be provided under a cell culture dish for driving the dish upward toward a fixed post member, thereby providing controlled compression of a tissue sample.... Agent: Kolisch Hartwell, P.C.

20080026425 - High-cell density fed-batch fermentation process for producing recombinant protein: Methods for producing proteins, for example, recombinant meningococcal 2086 proteins, using fed-batch fermentation with continuous input of an inducer after achieving a threshold parameter, and optionally continuous input of a carbon source, for example, a constant rate input, to improve protein yields, as well as high density protein compositions and... Agent: Hunton & Williams LLP Intellectual Property Department

20080026361 - Sperm cryoprotective media: Described herein is a composition that comprises a cryoprotectant; a membrane protectant that stabilizes or assists in stabilization of membranes of sperm; and a free radical scavenger (e.g., a reducing agent, an antioxidant).... Agent: Wolf Greenfield & Sacks, P.C.

20080026363 - Acetyl coa carboxylase 2 sequences and methods: The present invention relates generally to novel nucleotide and amino acid sequences, and more particularly to novel human acetyl CoA carboxylase 2 (ACC2) and rat ACC2 sequences. The sequences provided herein can be expressed in a recombinant format. Methods of isolating the ACC2 sequence are also provided, which can be... Agent: Louis J. Wille Bristol-myers Squibb Company

20080026366 - Biological fixative and method of using the biological fixative: A composition that includes an aldehyde, alcohol, and a ketone, the volumetric ratio of the alcohol to the ketone in the composition ranging from as low as about 0.8:1 to as high as about 4.5:1 and the volumetric ratio of the alcohol to the aldehyde in the composition ranging from... Agent: Law Office Of Phillip F. Fox

20080026365 - Hemostasis assay: Provided is a hemostasis assay comprising a reaction mixture comprising a blood product to be tested, a trigger molecule for inducing thrombin generation, a thrombin-specific substrate which, upon cleavage by thrombin, produces a measurable thrombin-specific signal, a trigger molecule for inducing plasmin generation, a plasmin-specific substrate which, upon cleavage by... Agent: Frommer Lawrence & Haug

20080026388 - Assay for measuring an enzyme's capability to modify supercoil topology of nucleic acids and modulators: Provided are methods, such as high throughput methods, of assessing or measuring the ability of an enzyme to modify the supercoil topology of a target nucleic acid, the methods comprising the steps of: (a) providing a solid support to which a capture nucleic acid is or may be immobilised, which... Agent: Speckman Law Group PLLC

20080026391 - Blood diagnosis method for dialysis patient and dialysis machine: Provided is a blood diagnosis method of providing a diagnostic marker which is general and which contributes to dialysis treatment and evaluation of clinical effects. The method includes a step of collecting blood samples from a dialysis patient before and/or after dialysis and a step of performing a gene diagnosis... Agent: Sughrue Mion, PLLC

20080026390 - Diagnosis of fetal abnormalities by comparative genomic hybridization analysis: The present invention provides systems, apparatuses, and methods to detect the presence of fetal cells when mixed with a population of maternal cells in a sample and to test fetal abnormalities, e.g. aneuploidy. The present invention involves performing comparative genomic hybridization (CGH) analysis when fetal cells are present in a... Agent: Wilson Sonsini Goodrich & Rosati

20080026397 - Electrochemical method to measure dna attachment to an electrode surface in the presence of molecular oxygen: The present disclosure provides methods and compositions for conducting an assay to detect a polynucleotide. In particular, ruthenium complexes having reduction potentials that do not coincide with the reduction potential of molecular oxygen are disclosed and amperometric techniques for their use are described. In preferred embodiments, the ruthenium complex is... Agent: Knobbe Martens Olson & Bear LLP

20080026372 - In vitro processes for producing multiple copies of primer sequence-free specific nucleic acid: This invention provides inter alia an in vitro process for producing multiple specific nucleic acid copies in which the copies are produced under isostatic conditions, e.g., temperature, buffer and ionic strength, and independently of any requirement for introducing an intermediate structure for producing the copies. In other aspects, the invention... Agent: Enzo Biochem, Inc.

20080026386 - Irf-5 haplotypes in systemic lupus erythematosus: Methods and materials involved in diagnosing SLE are provided herein. The methods and materials can be used to diagnose SLE and/or assess a mammal's susceptibility to develop SLE, based on the presence or absence of one or more IRF-5 variants.... Agent: Fish & Richardson P.C.

20080026396 - Method and nucleic acids for the analysis of astrocytomas: Chemically modified genomic sequences, oligonucleotides and/or PNA-oligomers for detecting the cytosine methylation state of genomic DNA. In addition, a method for ascertaining genetic and/or epigenetic parameters of genes for use in the characterization, classificaiton, differrentiation, grading, staging, treatment and/or diagnosis of astrocytomas, or the predisposition to astrocytomas.... Agent: Davis Wright Tremaine, LLP/seattle

20080026392 - Method for identifying novel genes: Methods and compositions for identifying novel genes that share regions of homology with known genes from target groups of genes of interest are provided. The methods comprise systematically designing oligonucleotide primers that are specific for regions of homology within the nucleotide sequences of a target group of known genes and... Agent: Alston & Bird LLP Pioneer Hi-bred International, Inc.

20080026395 - Method of detection of prostate cancer: The present invention provides methods and kits useful for detecting neplasia by measuring the methylation level of biomarkers, especially the promoter region of GSTP1 for the detection of prostate adenocarcinoma.... Agent: Lisa A. Haile, J.d., Ph.d. Dla Piper US LLP

20080026399 - Method of treating bladder and lower urinary tract syndromes: The present invention relates to bladder and lower urinary tract syndromes, particularly, irrative symptoms, and to a method of treating same using α1d-adrenergic receptor (α1ddAR) antagonists. The invention further relates to a method of screening compounds for their ability to serve as α1dAR selective antagonists.... Agent: Nixon & Vanderhye, PC

20080026393 - Method to produce single stranded dna of defined length and sequence and dna probes produced thereby: A method for producing a single stranded DNA (ssDNA) molecule of a defined length and sequence is disclosed. This method enables the preparation of, inter alia, probes of greater length than can be chemically synthesized. The method starts with a double stranded molecule, such as genomic, double stranded DNA (dsDNA)... Agent: Peters Verny , L.L.P.

20080026387 - Methods and compositions for detection of a target nucleic acid sequence utilizing a probe with a 3' flap: The invention provides compositions, kits and methods of generating a signal indicative of the presence of a target nucleic acid sequence in a sample by forming a cleavage structure. The cleavage structure is formed by incubating a sample containing a target nucleic acid with a downstream probe that forms a... Agent: Agilent Techologies Inc

20080026384 - Methods and compositions for the detection of bovine pregnancy: Provided herein are pregnancy specific marker genes, such as those shown in Tables I-III, and methods of detecting the same to determine bovine pregnancy.... Agent: Dann, Dorfman, Herrell & Skillman

20080026367 - Methods for genomic analysis: The present invention relates to business methods for discovery of therapeutic and diagnostic products by identifying variations that occur in the human genome, relating these variations to one another, and, ultimately, relating these variations to the genetic bases of phenotype such as disease resistance, disease susceptibility or drug response.... Agent: Perlegen Sciences, Inc. Legal Department

20080026394 - Methods of detecting one or more cancer markers: The present invention provides a methods and compositions for early diagnosis of cancer by rapid and specific detection of one or more cancer markers in a sample.... Agent: Patton Boggs LLP

20080026400 - Methods to identify biologically active agents and synergistic combinations: Methods are described using a particular type of assay system, the Multi-Pathway High Throughput Assay, in conjunction with a novel experimental strategy, whereby repeated cycles of experiments result in the identification of the most effective synergistic combinations of potential active agents from a library of materials. The novel experimental strategy... Agent: Ian R. Scott

20080026398 - Nucleic acid sequences for detecting genetic markers for cancer in a biological sample: Nucleic acid sequences for detecting the presence of nucleic acids, particularly mRNA, encoding human prostate-associated genetic markers encoding prostate-specific antigen (PSA), prostate specific membrane antigen (PSMA) or human kallikrein 2 (hK2) are disclosed. Preferred combinations of nucleic acid sequences amplifying and detecting the prostate-associated genetic markers RNA, used in methods... Agent: Gen Probe Incorporated

20080026385 - Oligonucleotides for cancer diagnosis: The present invention provides sets of oligonucleotides corresponding to genes encoding proteins involved in protein synthesis and/or stability or genes encoding proteins involved in the regulation of defence and/or chromatin remodelling for use in preparing transcript patterns particularly for cancer diagnosis. The invention also extends to such sets and kits... Agent: Sughrue Mion, PLLC

20080026401 - Polynucleotides encoding a novel testis-specific tubulin tyrosine-ligase-like protein, bgs42: The present invention provides novel polynucleotides encoding BGS-42 polypeptides, fragments and homologues thereof. Also provided are vectors, host cells, antibodies, and recombinant and synthetic methods for producing said polypeptides. The invention further relates to diagnostic and therapeutic methods for applying these novel BGS-42 polypeptides to the diagnosis, treatment, and/or prevention... Agent: Louis J. Wille Bristol-myers Squibb Company

20080026371 - Protein c and endothelial protein c receptor polymorphisms as indicators of subject outcome: The invention provides methods and kits for obtaining a prognosis for a subject having or at risk of developing an inflammatory condition and for identifying subjects having a greater benefit from treatment with an anti-inflammatory agent or an anti-coagulant agent. The method generally comprises determining a protein C and/or EPCR... Agent: Browdy And Neimark, P.l.l.c. 624 Ninth Street, Nw

20080026389 - Screen for anti-infective compounds: The present invention provides methods and compositions for identifying compounds useful as therapeutics and disinfectants. Such compounds would be particularly useful in treating Gram-positive bacterial infections. Such therapeutics would target the interaction of a tRNA molecule with an mRNA molecule, interrupting translation of the mRNA molecule and thus interfering with... Agent: Myers Bigel Sibley & Sajovec

20080026404 - Chimeric human t1r3 taste receptor polyptides and compositions containing same: Newly identified mammalian taste-cell-specific G protein-coupled receptors, and the genes and cDNA encoding said receptors are described. Specifically, T1R G protein-coupled receptors active in taste signaling, and the genes and cDNA encoding the same, are described, along with methods for isolating such genes and for isolating and expressing such receptors.... Agent: Hunton & Williams LLP Intellectual Property Department

20080026402 - Hematopoietin receptors hpr1 and hpr2: This invention relates to human and murine HPR1 and human and murine HPR2 polypeptides, new members of the hematopoietin receptor polypeptide family; to methods of making such HPR1 and HPR2 polypeptides; to non-human mammals in which the endogenous genomic sequences encoding HPR1 and/or HPR2 polypeptides have been partially or completely... Agent: Immunex Corporation Law Department

20080026403 - Methods, particles, and kits for determining activity of a kinase: Methods, particles and kits for determining kinase activity within a sample are provided. An embodiment of a method includes exposing a fluorescent particle to an assay, wherein the fluorescent particle includes a support substrate having one or more fluorescent materials and a peptide substrate coupled to the support substrate via... Agent: Daffer Mcdaniel LLP

20080026406 - Method for diagnosis of myelodysplastic syndromes (mds): The present invention provides a method of diagnosing a mylodysplastic syndrome, a method of ascertaining the clinical course of a myelodysplastic syndrome or a method of ascertaining response to a therapy regimen in a subject diagnosed with a myelodysplastic syndrome, and kits for effecting the same.... Agent: Pearl Cohen Zedek Latzer, LLP

20080026410 - Biomarkers for bladder cancer: The present invention provides protein-based biomarkers and biomarker combinations that are useful in qualifying bladder cancer status in a patient. In particular, the biomarkers of this invention are useful to classify a subject sample as bladder cancer or non-bladder cancer. The biomarkers can be detected by SELDI mass spectrometry.... Agent: Foley And Lardner LLP Suite 500

20080026407 - Covalent tethering of functional groups to proteins: A mutant hydrolase optionally fused to a protein of interest is provided. The mutant hydrolase is capable of forming a bond with a substrate for the corresponding nonmutant (wild-type) hydrolase which is more stable than the bond formed between the wild-type hydrolase and the substrate. Substrates for hydrolases comprising one... Agent: Schwegman, Lundberg, Woessner & Kluth, P.A.

20080026408 - Isolation of analytes: The disclosure features, inter alia, methods and compositions for detecting, isolating, and/or analyzing one or more analytes such as, but not limited to, biomolecules (e.g., proteins, glycoproteins, lipids (e.g., sphingolipids), and carbohydrates). Such methods and compositions are useful in, e.g., the discovery of novel proteins, or the amino acid sequence... Agent: Fish & Richardson PC

20080026409 - Salt-sensitive hypertension: Identification of compositions of biomarkers which diagnose and distinguish between salt-sensitive hypertension, salt-resistant hypertension, salt-independent hypertension and/or hypertensive disorders comprise identifying varying levels of biomarkers in each patient as compared to normal patients. Methods for the identification of novel biomarkers and determination of minimal maximally effective doses of pharmaceutical agents... Agent: Akerman Senterfitt

20080026412 - Beta-2 microglobulin as a biomarker for peripheral artery disease: The present invention provides β2 microglobulin as a biomarker for qualifying or assessing peripheral artery disease in a subject.... Agent: Townsend And Townsend And Crew LLP

20080026413 - Complexes and methods: The invention relates to a cell comprising an exogenous capture moiety on its cell surface, wherein said capture moiety is capable of supporting the attachment of an HLA molecule thereto. In another aspect the invention relates to a cell comprising a capture moiety on its cell surface, and an HLA... Agent: Frommer Lawrence & Haug

20080026415 - Methods for making cancer prognoses based on subcellular localization of biomarkers: This invention provides a method of making a prognosis for a patient afflicted with a type of cancer such as colon cancer, based upon quantification of biomarkers such as thymidylate synthase in subcellular compartments.... Agent: Cooper & Dunham, LLP

20080026417 - Assays for trichomonal and other hydrolases: The release by trichomonads of a hydrolase that hydrolyzes a narrowly defined class of substrates at a low pH without interference from hydrolases that are unrelated to trichomoniasis is the basis for a selective diagnostic assay for trichomoniasis that measures hydrolysis of any of these substrates by vaginal fluid at... Agent: Townsend And Townsend And Crew, LLP

20080026418 - Device and direct method for detecting antibiotic-inactivating factors: The present invention provides a method of determining the antibiotic susceptibility of a microorganism comprising the following steps. First, a culture of the microorganism whose susceptibility is to be determined is admixed with an antibiotic to which susceptibility is to be assayed, and a permeabilizing agent for the microorganism present... Agent: Stinson Morrison Hecker LLP Attn: Patent Group

20080026420 - Systems and methods for automated analysis of cells and tissues: Systems and methods for rapidly analyzing cell containing samples, for example to identify morphology or to localize and quantitate biomarkers are disclosed.... Agent: Cooper & Dunham, LLP

20080026426 - Anti-infective therapy: DNA isolates coding for human DNase and methods of obtaining such DNA are provided, together with expression systems for recombinant production of human DNase useful in therapeutic or diagnostic compositions.... Agent: Genentech, Inc.

20080026423 - Dep-1 receptor protein tyrosine phosphatase interacting proteins and related methods: Proteins are identified from human breast tumor cell lines (MDA-MB-231, T-47D and T-47D/Met) that interact specifically with the substrate-trapping mutant form of Density Enhanced Phosphatase-1 (DEP-1). These proteins include the functional component p120 catenin (p120ctn), the adaptor protein Gab 1, and the HGF/SF receptor Met. The invention relates to isolated... Agent: Seed Intellectual Property Law Group PLLC

20080026421 - Method for production of a compound in a eukaryotic cell: The present invention relates to a eukaryotic cell containing peroxisomes that are capable to fuse with a membrane-structure of the cell involved in the secretory pathway of the cell. In this way, the eukaryotic cell is able to release the peroxisomal content outside the cell. The invention also relates to... Agent: Nixon & Vanderhye, PC

20080026422 - Overexpression of aminoacyl-trna synthetases for efficient production of engineered proteins containing amino acid analogues: Methods for producing modified polypeptides containing amino acid analogues are disclosed. The invention further provides purified dihydrofolate reductase polypeptides, produced by the methods of the invention, in which the methionine residues have been replaced with homoallylglycine, homoproparglycine, norvaline, norleucine, cis-crotylglycine, trans-crotylglycine, 2-aminoheptanoic acid, 2-butynylglycine and allylglycine.... Agent: Seed Intellectual Property Law Group PLLC

20080026424 - Recombinant histidine-tagged inosine nonophosphate dehydrogenase polypeptides: The invention relates to a modified IMPDH polypeptide wherein the IMPDH polypeptide has a histidine tag and where the subdomain of the IMPDH polypeptide is modified so that the rate stability of the histidine-tagged, modified IMPDH polypeptide is maintained relative to the wild-type IMPDH polypeptide. The invention also relates to... Agent: Roche Diagnostics Operations Inc.

20080026427 - Human cdnas and proteins and uses thereof: The invention concerns GENSET polynucleotides and polypeptides. Such GENSET products may be used as reagents in forensic analyses, as chromosome markers, as tissue/cell/organelle-specific markers, in the production of expression vectors. In addition, they may be used in screening and diagnosis assays for abnormal GENSET expression and/or biological activity and for... Agent: Saliwanchik Lloyd & Saliwanchik A Professional Association

20080026428 - Method for producing nucleotide by fermentation: Nucleoside 5′-phosphate ester is produced by culturing a bacterium belonging to the genus Escherichia having an ability to produce nucleoside 5′-phosphate ester, in which ushA gene and aphA gene do not function normally, in a medium to produce and accumulate nucleoside 5′-phosphate ester in the medium, and collecting the nucleoside... Agent: Oblon, Spivak, Mcclelland Maier & Neustadt, P.C.

20080026430 - Instrument for heating and cooling: An instrument and method is provided that conveniently allows rapid amplification of nucleic acids.... Agent: Townsend And Townsend And Crew, LLP

20080026429 - Solution pre-treatment: A method of preparing a solution for use in DNA amplification reactions, including the steps of, mixing a binding agent with a base solution; subjecting the mixture (of a binding agent and base solution) to a stimulus which activates the binding agent, and upon activation, the binding agent binding to... Agent: Greer, Burns & Crain

20080026431 - Method for saccharification of woody biomass: Provided is an inexpensive and efficient saccharification method for woody biomass and a method of producing ethanol from woody biomass. A method for saccharification of woody biomass comprising hydrolysis treatment and an enzymatic treatment, in which a pretreatment process is performed prior to the enzymatic treatment and said pretreatment includes... Agent: Oblon, Spivak, Mcclelland Maier & Neustadt, P.C.

20080026432 - Novel l-threonine importer from corynebacterium and a preparation method of a strain producing l-threonine: The present invention relates to a preparation method of an L-threonine producing strain by utilizing a novel L-threonine importer identified from Corynebacterium glutamicum. The method can be advantageously used for the production of L-threonine by increasing the fermentation concentration of Lthreonine and the yield per unit thereof.... Agent: Birch Stewart Kolasch & Birch

20080026433 - Use of enzymatic resolution for the preparation of intermediates of pregabalin: Provided is the use of enzymatic resolution for the preparation of intermediates of pregabalin, including (3S)-cyano-5-methylhexanoic acid and salts thereof and R-(−)-3-(carbamoylmethyl)-5-methylhexanoic acid and salts thereof.... Agent: Kenyon & Kenyon LLP

20080026434 - Pufa polyketide synthase systems and uses thereof: Disclosed are the complete polyunsaturated fatty acid (PUFA) polyketide synthase (PKS) systems from the bacterial microorganisms Shewanella japonica and Shewanella olleyana, and biologically active fragments and homologues thereof. More particularly, this invention relates to nucleic acids encoding such PUFA PKS systems, to proteins and domains thereof that comprise such PUFA... Agent: Sheridan Ross PC

20080026435 - Pufa polyketide synthase systems and uses thereof: Disclosed are the complete polyunsaturated fatty acid (PUFA) polyketide synthase (PKS) systems from the bacterial microorganisms Shewanella japonica and Shewanella olleyana, and biologically active fragments and homologues thereof. More particularly, this invention relates to nucleic acids encoding such PUFA PKS systems, to proteins and domains thereof that comprise such PUFA... Agent: Sheridan Ross PC

20080026436 - Pufa polyketide synthase systems and uses thereof: Disclosed are the complete polyunsaturated fatty acid (PUFA) polyketide synthase (PKS) systems from the bacterial microorganisms Shewanella japonica and Shewanella olleyana, and biologically active fragments and homologues thereof. More particularly, this invention relates to nucleic acids encoding such PUFA PKS systems, to proteins and domains thereof that comprise such PUFA... Agent: Sheridan Ross PC

20080026437 - Pufa polyketide synthase systems and uses thereof: Disclosed are the complete polyunsaturated fatty acid (PUFA) polyketide synthase (PKS) systems from the bacterial microorganisms Shewanella japonica and Shewanella olleyana, and biologically active fragments and homologues thereof. More particularly, this invention relates to nucleic acids encoding such PUFA PKS systems, to proteins and domains thereof that comprise such PUFA... Agent: Sheridan Ross PC

20080026438 - Pufa polyketide synthase systems and uses thereof: The invention generally relates to polyunsaturated fatty acid (PUFA) polyketide synthase (PKS) systems isolated from or derived from non-bacterial organisms, to homologues thereof, to isolated nucleic acid molecules and recombinant nucleic acid molecules encoding biologically active domains of such a PUFA PKS system, to genetically modified organisms comprising PUFA PKS... Agent: Sheridan Ross PC

20080026439 - Pufa polyketide synthase systems and uses thereof: The invention generally relates to polyunsaturated fatty acid (PUFA) polyketide synthase (PKS) systems isolated from or derived from non-bacterial organisms, to homologues thereof, to isolated nucleic acid molecules and recombinant nucleic acid molecules encoding biologically active domains of such a PUFA PKS system, to genetically modified organisms comprising PUFA PKS... Agent: Sheridan Ross PC

20080026440 - Pufa polyketide synthase systems and uses thereof: The invention generally relates to polyunsaturated fatty acid (PUFA) polyketide synthase (PKS) systems isolated from or derived from non-bacterial organisms, to homologues thereof, to isolated nucleic acid molecules and recombinant nucleic acid molecules encoding biologically active domains of such a PUFA PKS system, to genetically modified organisms comprising PUFA PKS... Agent: Sheridan Ross PC

20080026441 - Production of probiotic bacteria using maple sap: Use of maple sap, down-graded maple syrup or mixture thereof as a carbon and energy source for the production of probiotic bacteria (e.g. Lactobacilli spp.) unexpectedly leads to an improvement in growth of the probiotic bacteria and an improvement in the yield of products produced by the bacteria.... Agent: National Research Council Of Canada 1200 Montreal Road

20080026442 - Ethanol production from biological wastes: The invention provides methods, apparatuses, and kits for producing ethanol and other alcohols. The methods involve fermenting organic material in a fermentation mixture to a biogas comprising methane; converting at least a portion of the biogas to synthesis gas comprising CO and H2; and contacting at least a portion of... Agent: Cyr & Associates, P.A.

20080026443 - Ethanol production from biological wastes: The invention provides methods, apparatuses, and kits for producing ethanol and other alcohols. The methods involve fermenting organic material in a fermentation mixture to a biogas comprising methane; converting at least a portion of the biogas to synthesis gas comprising CO and H2; and contacting at least a portion of... Agent: Cyr & Associates, P.A.

20080026444 - Process for producing protein-polymer complex: By reacting a protein conjugated with a polymer thereto with a compound having a mercapto group and eliminating a polymer ester-bound to a mercapto group of a cysteine residue, a protein-polymer complex can be produced without considerably reducing function of a protein.... Agent: Birch Stewart Kolasch & Birch

20080026445 - Oxygen scavenger coating composition: A coating composition having a multicopper oxidase enzyme and an oxidizable substrate in combination with a organic binder polymer which may be dispersed or dissolved in a non-aqueous liquid vehicle.... Agent: E I Du Pont De Nemours And Company Legal Patent Records Center

20080026447 - Assay for determining factor viia inhibitor concentration in plasma samples: This invention provides a method for determining the concentration of a factor VIIa inhibitor in a sample. A method for determining non specific binding of a factor VIIa inhibitor to proteins other than factor VIIa is also provided.... Agent: Louis J. Wille Bristol-myers Squibb Company

20080026446 - Combination of a growth factor and a protease enzyme: A composition combining an extracellular matrix-degrading protease and a growth factor related to epithelial cell functions together without inactivation or degradation of the growth factor by the protease. Preferably, the protease is plasmin/plasminogen or a related one, and the growth factor is KGF or a related one. The combination associates... Agent: Jackson Walker LLP

20080026448 - Production of hiv: The present invention provides methods for producing HIV and HIV antigen by propagating HIV, in particular HIV-1, in cells grown in chemically defined, protein free medium. The invention also provides HIV antigen produced by such methods.... Agent: Mcdermott, Will & Emery

20080026449 - Infectious bronchitis virus vaccine: Embodiments of the present invention generally relate to a novel attenuated infectious bronchitis virus (IBV) of GA-98 isolate. Further, other embodiments of the present invention generally relate to methods of immunizing avian against an infectious bronchitis virus. As well, further embodiments relate to method of making a vaccine and/or immunogenic... Agent: Intervet Inc. Patent Department

20080026450 - Method of leaching copper sulfide ores containing chalcopyrite: A method of recovering copper from copper sulfide ores, characterized by comprising: using a sulfuric acid solution having a chloride ion concentration adjusted to 6 g/L or more but less than 18 g/L and a pH adjusted to 1.6 or more but less than 2.5 as a leaching solution; and... Agent: Birch Stewart Kolasch & Birch

20080026451 - System for isolating biomolecules from a sample: The present invention provides an automated system for purification of a substance of interest. The system generally comprises an instrument for moving fluids through the system, a reagent pack for storing fluids, and a purification cartridge. The cartridge comprises two filtration units for binding substances based on different physical properties.... Agent: Latimer, Mayberry & MatthewsIPLaw, LLP

20080026452 - Device for determining distribution of glycation of erythrocytic membranes: A device for determining the distribution of glycation of erythrocytic membranes includes a container as a cell for electromagnetic-phoresis, a cathode ray and an anode ray which are connected to a direct current power supply, and a magnetic sheet which is placed outside the bottom of the container. The container... Agent: Akin Gump Strauss Hauer & Feld L.L.P.

20080026453 - Sample applicators for analytical assays: A liquid applicator for applying a liquid sample to a liquid receiving surface is disclosed. The liquid applicator comprises a bibulous line to hold a first predetermined volume of liquid for application to a liquid receiving surface. A second predetermined volume less than or equal to the first predetermined volume... Agent: Knobbe Martens Olson & Bear LLP

20080026454 - Biomolecule diagnostic devices and method for producing biomolecule diagnostic devices: A biosensor includes a substrate with a layer of receptive material disposed thereon overlying a layer containing a photo-reactive agent. The receptive material is specific for an analyte of interest. A pattern of active and inactive areas of the receptive material are defined in the receptive material layer by a... Agent: Dority & Manning, P.A.

20080026455 - Porous chamber for tissue culture in vitro: A porous chamber for tissue culture in vitro. The porous chamber includes a main body made of porous biologically absorbable polymer material, comprising an inner surface surrounding a hollow cavity, the hollow cavity having an aperture opened at an upper surface of the main body, wherein the aperture is communicated... Agent: Birch Stewart Kolasch & Birch

20080026456 - Method and apparatus for reducing pathogens in a biological sample: The present invention is directed to a method and apparatus for disinfection of biological samples, including semen and the like. In a first aspect of the present invention, a method for reducing pathogens in a biological sample includes passing the biological sample through a gradient including an enzyme suitable for... Agent: Suiter Swantz PC Llo

20080026458 - Chimeric and humanized antibodies to alpha5beta1 integrin that modulate angiogenesis: The present invention provides chimeric and humanized antibodies that specifically recognize α5β1 integrin, and methods for using the antibodies for reducing or inhibiting angiogenesis in a tissue. Also provided are methods of determining therapeutically acceptable doses of the antibodies and pharmaceutical compositions including the same.... Agent: Howrey LLP

20080026457 - Ungulates with genetically modified immune systems: The present invention provides ungulate animals, tissue and organs as well as cells and cell lines derived from such animals, tissue and organs, which lack expression of functional endogenous immunoglobulin loci. The present invention also provides ungulate animals, tissue and organs as well as cells and cell lines derived from... Agent: King & Spalding LLP

20080026459 - Isolation, selection and propagation of animal transgenic stem cells: Animal stem cells are obtained and maintained by culturing cells containing, in the genome, a selectable marker. Differential expression of the selectable marker enables preferential survival and/or division of the desired stem cells compared to the non-stem cells.... Agent: Finnegan, Henderson, Farabow, Garrett & Dunner LLP

20080026460 - Method for culturing stem cells: A three-dimensional microwell system that supports long term embryonic stem cell (ESCs) culture and formation of homogeneous embryoid bodies (EBs) is described. Microwell-cultured ESCs remain viable and undifferentiated for several weeks in culture and maintain undifferentiated replication when passaged to Matrigel®-coated, tissue culture-treated polystyrene dishes. Microwell-cultured ESCs maintain pluripotency, differentiating... Agent: Quarles & Brady LLP

20080026461 - Tissue-like organization of cells and macroscopic tissue-like constructs, generated by macromass culture of cells and the method of macromass culture: Three-dimensional tissue-like organization of cells by high cell-seeding-density culture termed as macromass culture is described. By macromass culture, cells can be made to organize themselves into a tissue-like form without the aid of a scaffold and three-dimensional macroscopic tissue-like constructs can be made wholly from cells. Tissue-like organization and macroscopic... Agent: Lackenbach Siegel

20080026462 - Meningeal-derived stem cells: Described herein are stem cells derived from the meninges; specifically, the dura mater, pia mater or arachnoid mater. Methods for isolating, differentiating and explanting these cells are described, as well. In particular embodiments, the stem cells of the present invention are differentiated into nerve cells, bone cells, cartilage cells and... Agent: Davis Wright Tremaine LLP/los Angeles

20080026463 - Matrix and method for isolation of hepatic progenitor cells: A method is provided of isolating and propagating hepatic progenitors in vitro on one or multiple extracellular matrix components comprising a collagen in polymeric form. A container for the isolation and propagation of the progenitors comprising culture dishes and/or bioreactors comprising the disclosed matrices is also provided.... Agent: Foley And Lardner LLP Suite 500

20080026464 - Nanotopographic compositions and methods for cellular organization in tissue engineered structures: The present invention relates to tissue engineered compositions and methods comprising nanotopographic surface topography (“nanotopography”) for use in modulating the organization and/or function of multiple cell types.... Agent: Ewards Angell Palmer & Dodge LLP

20080026465 - Cell culturing method using biomechanical stimulation loading and system therefor: To provide a cell culturing method employing biomechanical stimulation loading and its system for applying, in in vitro culture, stimulation similar to vertical weight load stimulation or sideward shear stress stimulation acting on tissues in an aboveground living body as repeated biomechanical stimulation to culture cells, culture tissues or a... Agent: Wenderoth, Lind & Ponack, L.L.P.

20080026466 - S-adenosyl-l-methionine synthetase promoter and its use in expression of transgenic genes in plants: A constitutive plant S-adenosyl-L-methionine synthetase (SAMS) promoter and subfragments thereof and their use in promoting the expression of one or more heterologous nucleic acid fragments in plants are described.... Agent: E I Du Pont De Nemours And Company Legal Patent Records Center

20080026467 - Novel agents for the prevention of leishmaniosis: The invention relates to nucleic acid constructions, characterized in that they comprise nucleic acids which are isolated in the sense position and which are capable of coding for an immunogenic protein of promastigotes or amastigotes of Leishmania, said nucleic acids responding to one of the sequences SEQ ID No. 1,... Agent: Nixon & Vanderhye, PC

20080020368 - Method and apparatus for exposing cells to different concentrations of analytes or drugs: A tapered microchannel structure allows individual cells to be reacted with a continuum of concentrations or dosages of an analyte or drug from one sample. A dual sandbag structure that divides up a tapered micro channel into thirds permits performing two simultaneous tests on two different sets of cells isolated... Agent: Robert K. Tendler

20080020369 - Neurotoxicity methods process: A method for testing the cytotoxicity of an implant comprising: providing neuronal cells in-vitro; providing one or more compounds of the implantable medical device; adding the one or more compounds from the implantable medical device to the in-vitro neuronal cells; and determining a measure of the cytotoxicity of the one... Agent: Advanced Neuromodulation Systems, Inc.

20080020373 - Composition for detecting the response of rectal adenocarcinomas to radiochemotherapy: A cDNA array (9984 genes) was used for expression profiling in rectal adenocarcinoma. The expression data were correlated to responsiveness to chemotherapy followed by radiotherapy. A set of 54 genes was found that were differentially expressed in responders vs. non-responders. The genes may be used as prognostic markers for determining... Agent: National Institutes Of Health C/o Venable LLP

20080020395 - Gamma secretase notch biomarkers: The present invention relates to the biomarker TFF-3 that measures γ-secretase mediated Notch processing. TFF-3 has utility in predicting and/or determining in vivo Notch-related toxicity associated with inhibition of Notch processing mediated by γ-secretase. The reagents and methods of the invention can be utilized before, after, or concurrently with, pre-clinical,... Agent: Louis J. Wille Bristol-myers Squibb Company

20080020380 - Method of denaturing and fragmenting dna or rna using ultrasound: A method and apparatus are provided for processing a DNA or RNA sample within a sample processing module. The method includes the steps of providing a sample well within the sample processing module that contains the DNA or RNA sample, coupling ultrasonic energy from an external source into the sampling... Agent: Welsh & Katz, Ltd. Jon P. Christensen

20080020372 - Methods for detecting and treating cancer: Methods and kits for detecting cancer and monitoring cancer progression are described. The method involves analyzing a sample containing nucleic acids or proteins from a patient for decreased expression of endoglycan and/or increased expression of podocalyxin.... Agent: Bereskin And Parr

20080020384 - Stabilizing composition and stabilizing method of coelenterazine solution for high-throughput measurement of luciferase activity: The present invention relates to a stabilization composition of coelenterazine or an analog thereof, a kit, a method for measuring a coelenterazine-based biological luminescence, containing coelenterazine or the analog thereof and an antioxidant.... Agent: Knobbe Martens Olson & Bear LLP

20080020401 - Immunosuppressant drug extraction reagent for immunoassays: An improved extractive reagent composition and method for extracting an immunosuppressant drug, such as sirolimus, tacrolimus or cyclosporine, from blood samples while yielding a test sample extract that has low vapor pressure and is compatible with immunoassay components. The inventive reagent composition comprises dimethyl sulfoxide (DMSO), at least one divalent... Agent: Vysis, Inc Patent Department

20080020400 - Oxidized apoa-i determination by mass spectrometry: Methods are provided for the detection and quantitation of proteins generally and apolipoprotein A-I and oxidized derivatives thereof in particular. Further provided are methods for the assessment of the risk cardiovascular disease in a subject, wherein the assessment is based on the amount of oxidized and unoxidized apolipoprotein A-I in... Agent: Barry S. Wilson Foley & Lardner LLP

20080020408 - Anti-ube4a/ufd2b polyclonal antibody and its use thereof as diagnostic and prognostic marker of 11q23 region alterations: The subject of this invention is an anti-UBE4A/Ufd2b polyclonal antibody. Moreover, this invention refers to the use of said antibody as a diagnostic and prognostic marker of alterations in the 11q23 region.... Agent: Pearne & Gordon LLP

20080020407 - Identification, isolation and elimination of cancer stem cells: Isolated populations of leukemic stem cells are provided. The cells are useful for experimental evaluation, and as a source of lineage and cell specific products, and as targets for the discovery of factors or molecules that can affect them. Detection of leukemic stem cells is useful in predicting disease progression,... Agent: Bozicevic, Field & Francis LLP

20080020428 - Method of producing meningococcal meningitis vaccine for neisseria meningitidis serotypes a,c,y, and w-135: Methods for producing quadrivalent meningococcal meningitis polysaccharide and conjugate vaccines for serotypes A, C, Y and W-135 disclosed. Neisseria meningitidis fastidious medium was designed to maximize the yield of capsular polysaccharides and generate minimal cellular biomass and endotoxin in a short duration of fermentation. The crude polysaccharides are isolated, purified,... Agent: Jeeri R. Reddy

20080020430 - Enzymatic process for preparing aminoacyl esters of monosaccharides: The present invention particularly relates to lipase mediated synthesis of aminoacyl esters of monosaccarides from unprotected amino acids and monosaccharides in non-polar solvents using lipases.... Agent: Ladas & Parry

20080020370 - Heteroduplex tracking assay: A change in viral tropism occurs in many HIV positive individuals over time and may be indicated by a shift in coreceptor use from CCR5 to CXCR4. The shift in coreceptor use to CXCR4 has been shown to correlate with increased disease progression. In patients undergoing HAART, the predominant populations... Agent: Frommer Lawrence & Haug

20080020371 - Reverse genetics system: The complete genomic sequence of maize fine streak virus (MFSV), a negative-strand RNA virus that infects plants and insects, is disclosed. The inventors have characterized the MFSV genome and identified the leader and trailer sequences, seven open reading frames as well as the functions of some of the encoded proteins,... Agent: Quarles & Brady LLP

20080020382 - Biological microbeads for various flow cytometric applications: The invention relates to biological microbeads, and in certain embodiments, to the use thereof in flow-cytometric applications. Biological microbeads are fixed cells that may be surface-modified with proteins or other molecules that have specific binding properties. These surface-bound proteins may bind to target compounds bearing fluorescent labels that facilitate detection... Agent: Davis Wright Tremaine LLP/los Angeles

20080020387 - Biomarker-optimized adhd treatment: Methods of predicting patient responsiveness to treatment of attention-deficit/hyperactivity disorder (ADHD) with selective norepinephrine reuptake inhibitors; identifying individuals requiring a higher than normal dose of atomoxetine for treating ADHD; and predicting patient responsiveness to treatment of neuropsychiatric diseases or disorders responsive to treatment with selective norepinephrine reuptake inhibitors are provided.... Agent: Eli Lilly & Company

20080020396 - Composition for amplifying nucleic acids: The invention relates to a concentrated and buffered liquid composition for amplifying nucleic acids, comprising at least one dNTP, at least one enzyme required for the amplification, at least one oligonucleotide primer, and at least one fluorescent nucleotide probe, in the presence of a polyol and/or of polyvinylpyrrolidone (PVP).... Agent: Jacobson Holman PLLC

20080020398 - Cross-reactive primers for amplifying the nucleic acids of hiv-1 and hiv-2: Compositions, methods and kits for detecting the nucleic acids of HIV-1, HIV-2, or the combination of HIV-1 and HIV-2. Particularly described are oligonucleotides that are useful as hybridization probes and amplification primers, including cross-reacting hybridization probes and cross-reacting amplification primers, for detecting very low levels of viral nucleic acids.... Agent: Gen Probe Incorporated

20080020390 - Detecting fetal chromosomal abnormalities using tandem single nucleotide polymorphisms: The invention provides tandem single nucleotide polymorphisms and methods for their use, for example, in diagnosing Down Syndrome.... Agent: Viksnins Harris & Padys Pllp

20080020379 - Diagnosis and prognosis of infectious diseases clinical phenotypes and other physiologic states using host gene expression biomarkers in blood: The present invention provides a specific set of gene expression markers from peripheral blood leukocytes that are indicative of a host response to exposure, response, and recovery infectious pathogen infections. The present invention further provides methods for identifying the specific set of gene expression markers, methods of monitoring disease progression... Agent: Naval Research Laboratory Associate Counsel (patents)

20080020376 - Enriched cdna expression libraries and methods of making and using same: Disclosed are cDNA expression libraries enriched for cDNAs that encode, for example, secretory and membrane-bound proteins and to methods for making such libraries. Also disclosed are methods for using the cDNA expression libraries disclosed herein to screen for proteins, nucleic acids, and agents that modulate the function and/or expression of... Agent: Needle & Rosenberg, P.C.

20080020378 - Fluorescent base analogues' usage in the characterization of nucleic acid molecules and their interactions: This invention provides methods, apparatus and kits for the quantitative and qualitative characterization of the nucleic acid molecule's behavior by modify the nucleic acid molecules to incorporate selected fluorescent base analogues (FBAs). The methods generally place one or more fluorescent base analogue into a nucleic acid molecule (e.g., an oligonucleotide)... Agent: Yaodong Chen

20080020383 - Haplotype markers and methods of using the same to determine response to treatment: The present invention relates to methods for diagnosing or predicting responsiveness to treatment, such as Alefacept, by determining the presence of a nucleotide at one or more polymorphic sites within a haplotype marker. The present invention identifies multiple haplotypes that are associated with response to Alefacept. The haplotype markers identified... Agent: Leydig Voit & Mayer, Ltd

20080020393 - Iron regulating protein-2 (irp-2) as a diagnostic for neurodegenerative disease: The present invention relates to the discovery of markers for neurodegenerative disease. More particularly, it was discovered that forms of IRP-2 protein that are unable to undergo oxidation at critical cysteine residues are diagnostic for neurodegenerative disease including, but not limited to Alzheimer's disease. Embodiments include nucleic acids that encode... Agent: Knobbe Martens Olson & Bear LLP

20080020394 - Kunitz domain library: Disclosed are libraries, vectors, phage particles, host cells, and methods for displaying a Kunitz domain. The libraries can include Kunitz domains that vary in at least two interaction loops with respect to one another. Varied Kunitz domains can be displayed on phage at a low valency.... Agent: Fish & Richardson PC

20080020391 - Lateral flow methods and devices for detection of nucleic acid binding proteins: Methods and devices are provided for detecting the presence or absence of nucleic acid binding proteins, such as NMP22, and other proteins, in bodily fluids.... Agent: J. Gary Mohr (datascope)

20080020388 - Method for differentiating between multiple sclerosis subtypes: Disclosed herein is a method for differentiating between multiple sclerosis subtypes in a patient. The method comprises a) determining an amount of an IAP gene expression level in a blood sample obtained from the patient; and b) correlating the amount of the IAP gene expression level in the blood sample... Agent: Philip Swain, Phd C/o Gowling Lafleur Henderson

20080020375 - Method of adjudicating on prostate cancer: The present invention relates to a method for determining a subject having a risk of having developed or developing prostate cancer, which comprises the following steps: a) a step of analyzing the presence/absence or level of mutation in the PCA-1 gene derived from the subject; and b) a step of... Agent: Wenderoth, Lind & Ponack, L.L.P.

20080020386 - Methods and apparatus for genotyping: A method for determining the human leukocyte antigen (HLA) genotype of a nucleic acid sample, comprises: contacting a nucleic acid sample with at least one nucleic acid primer set and subjecting the mixture to a nucleic acid amplification reaction; determining the size of any amplification products produced in the amplification... Agent: Dinsmore & Shohl LLP

20080020389 - Methods of detecting a target nucleic acid: The invention relates to compositions and methods of generating a signal indicative of the presence of a target nucleic acid in a sample, where the method includes forming a cleavage structure by incubating a sample comprising a target nucleic acid with a probe. The invention also includes the steps of... Agent: Agilent Techologies Inc

20080020381 - Methods, compositions, and systems for the identification of species-specific or developmental stage-specific insecticides: Ecdysteroid action in Drosophila melanogaster and other insects is mediated by the dimerization of two nuclear receptors, the ecdysone receptor (EcR) and Ultraspiracle (USP), which regulate the transcription of target genes. Disclosed are nucleic acid constructs to identify insecticides having the ability to modify insect development and growth in a... Agent: Kilpatrick Stockton LLP

20080020392 - Motion resolved molecular sequencing: Methods are provided for determining the sequence of a polynucleotide by tracking the motion of a nucleotide-selective, processive enzyme along a template, in a reaction mixture that alters the enzyme movement in a base specific manner. The movement trace of the enzyme is collected, and arranged in temporal order. The... Agent: Bozicevic, Field & Francis LLP

20080020397 - Optimization of crossover points for directed evolution: Methods and devices for more efficiently engineering diversity into recombinant polypeptides and/or nucleic acids are provided herein. For example, a variety of methods of selecting and/or assessing potential crossover sites in an amino acid sequence or a nucleotide sequence are provided, as well as the resulting chimeric product sequences. These... Agent: Beyer Weaver LLP

20080020377 - Peptide-based method for monitorting gene expression in a host cell: The present invention relates a method for monitoring the expression level of a gene in a host cell by modulating the activity of a regulatory biomolecule, comprising the steps of: (a) transforming a cell expressing a regulatory biomolecule with a nucleic acid molecule comprising an open reading frame encoding an... Agent: Reed Smith LLP

20080020374 - Reagents, kits and methods for immunodetection of epitopes on molecules: Methods for detecting and/or quantifying molecules expressing a selected epitope in a sample are disclosed. Methods for profiling proteins in a cell lysate are also disclosed. Kits for detecting and/or quantifying molecules expressing a selected epitope in a sample and kits for profiling proteins in a cell lysate are also... Agent: Cozen O'connor

20080020385 - Use of a genetic modification in the human gnaq gene for predicting risk of disease, the course of disease, and reaction to treatments: In order to determine the risk of disease, the course of a disease, the action of medicaments, the side-effects of medicaments and drug targets, a base substitution is identified in the non-translated region 5′ of the gene for the Gαq sub-unit of human G proteins, preferably the presence of two... Agent: Amster, Rothstein & Ebenstein LLP

20080020403 - Nucleic acid encoding a novel prostaglandin receptor protein and methods of use thereof: Described herein is a novel member of the prostanoid receptor family, a guinea pig prostaglandin D2 receptor. Described are the receptor, the nucleic acid that encodes it, and various uses for both.... Agent: Andrea Q. Ryan Sanofi-aventis U.s. LLC

20080020402 - Polypeptide multilayer films and methods: Polypeptide multilayer films comprising a hydrophobic designed polypeptide, methods of making the polypeptide multilayer films, and methods of designing the hydrophobic polypeptide are disclosed.... Agent: Cantor Colburn, LLP

20080020404 - Search for cancer markers by a novel screening method: The present invention relates to a method of concentrating low molecular weight peptides in the supernatant of serum-free cultured cells, said method comprising allowing the peptides to bind to a strong cation exchanger under an acid condition, and eluting them under an alkali condition to concentrate the peptide. Furthermore, peptides... Agent: Foley And Lardner LLP Suite 500

20080020399 - Use of irs-polypeptides for identifying of pharmaceutically active compounds: An assay for determining the ability of an enzyme, functional fragment, or functional derivative thereof to modify the phosphorylation status of a biotinylated polypeptide.... Agent: Andrea Q. Ryan Sanofi-aventis U.s. LLC

20080020406 - Antibodies to a human kchannel and therapeutic applications thereof: The present invention relates to a novel human K+ ion channel, to nucleic acid molecules encoding the same and to vectors comprising said nucleic acid molecules. The invention additionally relates to antibodies specifically directed to the novel K+ ion channel and to pharmaceutical compositions and diagnostic kits containing at least... Agent: Ropes & Gray LLP Patent Docketing 39/361

20080020409 - Analytical platform and method for generating protein expression profiles of cell populations: The present invention is related to analytical platforms and methods performed therewith for generating qualitative and/or quantitative protein expression profiles, in particular differential protein expression profiles, of cell populations comprising: generating lysates of one or more populations of cells, the lysates comprising a plurality of proteins expressed by the respective... Agent: Norris, Mclaughlin & Marcus, Pa

20080020410 - Assays for detection of bacillus anthracis: This invention provides novel methods, reagents, and kits that are useful for detecting B. anthracis. The methods are based on the discovery of binding agents, including recombinant polyclonal antibodies, which bind to the surface array protein of B. anthracis.... Agent: Townsend And Townsend And Crew, LLP

20080020405 - Protein binding determination and manipulation: This invention addresses method for determining protein binding sites, molecules which bind to such sites and molecules which inhibit binding to particular protein binding sites. Also considered are conjoined molecules for enhanced binding to specific protein binding sites.... Agent: Thomas M. Saunders Brown Rudnick Berlack Israels LLP

20080020411 - Porous material, composition containing porous material, and sensor comprising porous material: A porous material containing mesopores with immobilized glucose dehydrogenase is provided. The pore size is at least 10 nm.... Agent: Fitzpatrick Cella Harper & Scinto

20080020412 - Aurora expression constructs: The present invention is directed to Aurora constructs that are useful for structural and functional studies of Aurora. Specifically, engineered Aurora enzymes are provided, along with polynucleotides encoding said enzymes, vectors comprising said polynucleotides, and host cells comprising the vectors. In addition, a process of using the engineered Aurora enzymes... Agent: Heller Ehrman LLP

20080020413 - Crystalline visfatin and methods therefor: Crystals of nicotinamide phosphoribosyltransferase, methods of making the crystals, and methods of using the crystals are disclosed. The three-dimensional structures of NMPRTases are also disclosed. Also disclosed are methods for utilizing a crystal structure of an NMPRTase for identifying, designing, selecting, or testing molecules which affect NMPRTase activity, which can... Agent: Sonnenschein Nath & Rosenthal LLP

20080020414 - Cyfra 21-1 as a marker for colorectal cancer: The present invention relates to the assessment of colorectal cancer. It discloses the use of protein CYFRA 21-1 in the assessment of colorectal cancer. It also relates to a method for assessing colorectal cancer in vitro using a liquid sample, derived from an individual by measuring CYFRA 21-1 in said... Agent: Roche Diagnostics Operations Inc.

20080020415 - Diacylglycerol acyltransferases for alteration of polyunsaturated fatty acids and oil content in oleaginous organisms: Acyltransferases are provided, suitable for use in the manufacture of microbial oils enriched in omega fatty acids in oleaginous yeast (e.g., Yarrowia lipolytica). Specifically, genes encoding diacylglycerol acyltransferase (DGAT1) have been isolated from Y. lipolytica and Mortierella alpina. These genes encode enzymes that participate in the terminal step in oil... Agent: E I Du Pont De Nemours And Company Legal Patent Records Center

20080020416 - Enzyme and preparation method: A method for preparing a soluble protein comprising urokinase-type plasminogen activator (uPA) or an active fragment thereof, or a variant of either of these which has uPA activity, which method comprises contacting said protein with a buffer at a pH of from 8.5-10.5, said buffer comprising a reducing agent and... Agent: Astrazeneca R&d Boston

20080020417 - Biological dataset profiling of asthma and atopy: Methods and systems for evaluating biological dataset profiles relating to asthma and other atopic conditions are provided, where datasets comprising information for multiple cellular parameters are compared and identified, and used in the evaluation of candidate pharmacologic agents for suitability as therapeutic agents.... Agent: Bozicevic, Field & Francis LLP

20080020418 - Cftr polypeptides, fragments thereof and methods of use to overcome biosynthetic misprocessing: The invention relates to biosynthetic maturation of cell surface polypeptides and, more specifically, to particular CFTR polypeptides which exhibit increased transport to the cell surface and tripeptide amino acid sequences that promote or enhance transport of export-incompetent CFTR to the cell surface.... Agent: Fish & Richardson P.C.

20080020419 - The use of saccharomyces cerevisiae erg4 mutants for expressing mammalian glucose transporter: The invention relates to yeast strains in which a human GLUT4 transport or a human GLUT1 transporter can be functionally expressed and to particular GLUT4 transport proteins which can be functionally expressed particularly readily in yeast strains.... Agent: Andrea Q. Ryan Sanofi-aventis U.s. LLC

20080020421 - Methods of screening agents for cytotoxic and antimicrobial activity: The present invention provides for methods of screening agents for cytotoxic and/or anti-microbial activity. In particular embodiments, the loss of a fluorescent signal from the treated cells, due to cell death or inhibition of growth, is measured for several different cell types in parallel.... Agent: Fulbright & Jaworski L.L.P.

20080020420 - Oligosaccharides in a test system for the determination of the presence of an antibiotic in a fluid: Provided is a test system, a test method and a test kit for the determination of the presence of an antibiotic in a fluid based on a test medium comprising a test organism, at least one substance that provides a solid state, nutrients and an indicator, wherein the end-pH of... Agent: Nixon & Vanderhye, PC

20080020422 - Methods and compositions for the detection of microbial contaminants: The invention provides methods and compositions for the detection and/or quantification of a microbial contaminant, for example, a bacterial endotoxin or a glucan, in a sample. In particular, the invention provides a test cartridge useful in the practice of hemocyte lysate-based assays for the detection and/or quantification of a microbial... Agent: Goodwin Procter LLP Patent Administrator

20080020423 - Enzyme reactions in miniemulsions: The present invention is directed towards an asymmetric enzymic process for preparing optically active organic compounds. The process according to the invention is carried out in what are termed miniemulsions. The invention also relates to an enzymic reaction mixture which exhibits a miniemulsion.... Agent: Law Office Of Michael A. Sanzo, LLC

20080020424 - Heteromeric umami t1r1/t1r3 taste receptors and isolated cells that express same: The present invention relates to the discovery that the T1R receptors assemble to form functional taste receptors. Particularly, it has been discovered that co-expression of T1R1 and T1R3 results in a taste receptor that responds to umami taste stimuli, including monosodium glutamate. Also, it has been discovered that co-expression of... Agent: Hunton & Williams LLP Intellectual Property Department

20080020427 - Method for producing alpha-1, 6-branched alpha-1, 4-glucans from sucrose: Nucleic acid molecules which encode a branching enzyme from a bacterium of the genus Neisseria, vectors, host cell, plant cells and plants containing said nucleic acid molecules as well as starch obtainable from the plants described are described. Furthermore, an in-vitro method for producing α-1,6-branched α-1,4-glucans on the basis of... Agent: Birch Stewart Kolasch & Birch

20080020426 - Novel glucose dehydrogenase: The present invention provides glucose dehydrogenase which is excellent in heat resistance and substrate specificity and is not affected by dissolved oxygen. Specifically, the present invention relates to glucose dehydrogenase characterized by being derived from an eukaryotic organism and keeping 90% or more activity after being treated with heat at... Agent: Leydig Voit & Mayer, Ltd

20080020425 - Polypeptides having lipase activity and polynucleotides encoding same: The present invention relates to isolated polypeptides having lipase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides.... Agent: Novozymes, Inc.

20080020429 - Method for producing alpha-1, 6-branched alpha-1, 4-glucans from sucrose: Nucleic acid molecules which encode a branching enzyme from a bacterium of the genus Neisseria, vectors, host cell, plant cells and plants containing said nucleic acid molecules as well as starch obtainable from the plants described are described. Furthermore, an in-vitro method for producing α-1,6-branched α-1,4-glucans on the basis of... Agent: Birch Stewart Kolasch & Birch

20080020431 - Methods and kits for nucleic acid amplification: Compositions and methods are provided for amplifying nucleic acid molecules. The nucleic acid molecules can be used in various research and diagnostic applications, such as gene expression studies involving nucleic acid microarrays.... Agent: J. Gary Mohr (datascope)

20080020432 - Method for producing alpha-1, 6-branched alpha-1, 4-glucans from sucrose: Nucleic acid molecules which encode a branching enzyme from a bacterium of the genus Neisseria, vectors, host cell, plant cells and plants containing said nucleic acid molecules as well as starch obtainable from the plants described are described. Furthermore, an in-vitro method for producing α-1,6-branched α-1,4-glucans on the basis of... Agent: Birch Stewart Kolasch & Birch

20080020433 - Process for producing amino acids: The present invention provides a microorganism obtainable by introducing a DNA coding for NADH dehydrogenase constituting a NADH dehydrogenase complex whose number of dischargeable proton molecules per electron is zero among NADH dehydrogenase complexes functioning as a proton pump in electron transport systems of aerobic bacteria, and an industrially advantageous... Agent: Fitzpatrick Cella Harper & Scinto

20080020434 - Polypeptides and biosynthetic pathways for the production of stereoisomers of monatin and their precursors: Monatin and certain stereoisomers of monatin, such as R,R monatin and S,R monatin, as well as salts thereof, are produced using polypeptides and biosynthetic pathways. These polypeptides and biosynthetic pathways are also useful in the production of R-2-hydroxy-2-(indoly-3-ylmethyl)-4-keto glutaric acid, an intermediate that is formed in certain monatin synthesis pathways,... Agent: Sterne, Kessler, Goldstein & Fox P.l.l.c.

20080020435 - Aldolases, nucleic acids encoding them and methods for making and using them: This invention relates to polypeptides having aldolase activity, including pyruvate activity such as, without limitation, HMG and/or KHG aldolase activity, polynucleotides encoding these polypeptides, and methods of making and using these polynucleotides and polypeptides. In some embodiments, the invention is directed to polypeptides having aldolase activity, including pyruvate activity such... Agent: Sterne, Kessler, Goldstein & Fox P.l.l.c.

20080020436 - Novel gene encoding formate dehydrogenases d & e and method for preparing succinic acid using the same: Nucleotide sequences encoding formate dehydrogenases D & E and a method for preparing succinic acid using the same, more particularly, formate dehydrogenases D & E converting formate to carbon dioxide and hydrogen, fdhD and fdhE nucleotide sequences encoding the formate dehydrogenases D & E, recombinant vectors containing the nucleotide sequences,... Agent: Intellectual Property / Technology Law

20080020437 - Apparatus and method for producing fuel ethanol from biomass: Cellulosic biomass at the growing site is pulverized and hydrolyzed in collectors which transfer the comminuted biomass into pretreatment units. In the pretreatment units enzymes and/or other agents are added to the pretreatment units to disassociate the lignin from the cellulose and hemicellulose, and to further decompose the biomass to... Agent: Dann, Dorfman, Herrell & Skillman

20080020438 - Diterpene-producing unicellular organism: The present invention is directed to a unicellular organism system, such as a yeast, for producing geranylgeranyl pyrophosphate and a diterpene in vivo. The yeast cell preferably comprises an inducible nucleic acid sequence encoding geranylgeranyl pyrophosphate synthase, an inducible nucleic acid sequence encoding a soluble form of HMG-CoA reductase, a... Agent: Baker Botts L.L.P. Patent Department

20080020439 - Enzymes as corrosion inhibitors by removal of oxygen dissolved in water: The invention relates to a new process for water de-oxygenation, for application in aerosol products. The process involves the use of an enzymatic system based on an oxidase enzyme a substrate for the oxidase enzyme and catalase. These two enzymes consume oxygen by a two step reaction with the substrate... Agent: Norris, Mclaughlin & Marcus

20080020440 - Method of sequestering and/or purifying a polypeptide: The present invention relates to DNA and polypeptide constructs and related methods useful for sequestering and/or purifying polypeptides. In particular, the invention relates to hybrid polypeptides comprising a polypeptide of interest linked to a polymerisable polypeptide, a method of sequestering and/or purifying a polypeptide of interest using the hybrid polypeptide... Agent: Protigene Pty Ltd

20080020441 - Intact minicells as vectors for dna transfer and gene therapy invitro and invivo: A composition comprising recombinant, intact minicells that contain a therapeutic nucleic acid molecule is disclosed. Methods for purifying a preparation of such minicells also are disclosed. Additionally, a genetic transformation method is disclosed, which comprises (i) making recombinant, intact minicells available that contain a plasmid comprised of a first nucleic... Agent: Foley And Lardner LLP Suite 500

20080020442 - Nucleic acid and amino acid sequences relating to streptococcus pneumoniae for diagnostics and therapeutics: The invention provides isolated polypeptide and nucleic acid sequences derived from Streptococcus pneumoniae that are useful in diagnosis and therapy of pathological conditions; antibodies against the polypeptides; and methods for the production of the polypeptides. The invention also provides methods for the detection, prevention and treatment of pathological conditions resulting... Agent: Hamilton, Brook, Smith & Reynolds, P.C.

20080020444 - Methyltransferase genes and uses thereof: The present invention relates to genes associated with the tocopherol biosynthesis pathway. More particularly, the present invention provides and includes nucleic acid molecules, proteins, and antibodies associated with genes that encode polypeptides that have methyltransferase activity. The present invention also provides methods for utilizing such agents, for example in gene...