Chemistry: molecular biology and microbiology inventions 10/06

20060240399 - Long-term shelf preservation of cells and multicellular specimens by vitrification: The method of preservation by vitrification, described in the present application, provides for storage of samples at higher temperatures than in conventional methods and can be applied to cells, multicellular tissues, organs and organisms. The method of the present invention includes preparing a solution of vitrification non-permeating co-solutes (amino acids,...

20060240401 - Handheld raman body fluid analyzer: Methods and apparatus for in vitro detection of an analyte in a body fluid sample using low resolution Raman spectroscopy are disclosed. The body fluid analyzer includes a disposable strip for receiving a sample of body fluid on a target region, the target region including gold sol-gel to provide surface...

20060240400 - High-density cell array board, process for producing the same and method of using the same: Culture substrates, cell arrays, automatic chemical dispensers and assay systems necessary for conveniently assaying multiple chemicals such as drugs and toxic agents are provided. Herein used is a substrate for a high-density cell array characterized in that it has a surface comprising an ordered array of discrete microdomains coated with...

20060240402 - Marine invertebrate gametes and embryos as an assay system for therapeutic screening: The invention provides methods of using of marine invertebrate gametes and preparations thereof for drug discovery and therapeutic screening....

20060240404 - Methods of isolating genes encoding proteins of specific function and of screening for pharmaceutically active agents: A method of isolating polynucleotides encoding polypeptides affecting an organization of a subcellular organelle or structure of interest is provided. The method comprises: (a) expressing within a plurality of cells an expression library including a plurality of expression constructs each encoding a polypeptide of interest; (b) highlighting the subcellular organelle...

20060240403 - Tape magazine for a hand-held device: The present invention concerns a tape magazine especially for a hand-held device for analysing a body fluid with a test tape that can be unwound from a storage unit and wound onto a waste unit where the waste unit can be driven in order to wind forward the test tape....

20060240412 - Compositions for use in identification of adenoviruses: The present invention provides compositions, kits and methods for rapid identification and quantification of adenoviruses by molecular mass and base composition analysis....

20060240407 - Detection and identification of enteroviruses: Disclosed herein are methods of using enterovirus-specific primers for the detection and identification of enterovirus infection. Also provided herein are isolated nucleic acid molecules and kits useful for detection and diagnostic testing of enterovirus infection in a subject....

20060240414 - Genetically engineered glutaminase and its use in antiviral and anticancer therapy: DNA encoding a therapeutically suitable glutaminase has been molecularly cloned. This allows one to obtain a polypeptide which is a therapeutically suitable glutaminase free of contaminating endotoxin. It has been found that this polypeptide is a potent anti-viral agent and when coupled to an anti-tumor monoclonal antibody is a potent...

20060240413 - Medical test probe for cell sample collection: A device and method are disclosed for collecting biological samples. One embodiment includes a device for collecting biological samples comprising a probe having a cell collector located on a distal end, the probe having a plurality of distinct raised protrusions....

20060240405 - Method for assaying replication of hbv and testing ... drugs: A method for measuring the replication capacity of HBV, e.g. HBV present in a biological sample, possibly in the presence of a pharmaceutical product, in particular an antiviral agent, the method comprising: (a) possibly extracting nucleic acids contained in the biological sample; (b) PCR amplifying HBV nucleic acids using at...

20060240409 - Method for extraction and identification of nucleic acids: The invention provides a method for extracting nucleic acids from a sample. The sample contains cells, viruses, or both cells and viruses. The method include adding a lysing solution containing a detergent to the sample to lyse the cells or viruses to form a lysate, adding alcohol to the lysate...

20060240406 - Method for the detection of proteolytic enzymes: The present invention provides an improved protease assay in which the proteases are detected on basis of their capability to cleave a modified pro-caspase which will yield an activated caspase which can then further be detected. Also part of the invention are the modified pro-caspases and kits comprising said modified...

20060240408 - Method of purifying nucleic acid using silver nanoparticles: Provided is a method of purifying a target substance using silver nanoparticles. The method includes: mixing a sample containing molecules having a thiol group with the silver nanoparticles to obtain a complex of the molecules having the thiol group with the silver nanoparticles; and isolating and removing the complex from...

20060240411 - Multiplex microparticle system: Arrays of microparticle populations, each population labeled with a single fluorescent dye, are provided for use in multiplex assays. The populations form a virtual multidimensional array wherein each microparticle is identified by fluorescence intensity in two different fluorescence detection channels. The arrays are useful in a variety of assays, including...

20060240410 - Retroviral protease inhibitor combinations: The present invention is directed to a method for the treatment of mammalian retrovirus infections, such as HIV, using combinations of retroviral protease inhibitors which are effective in preventing the replication of the retroviruses in vitro or in vivo. This invention, in particular, relates to protease inhibitor compounds used in...

20060240415 - Screening compound libraries using an optical fiber array device capable of simultaneously performing multiple functional assays: Disclosed is a method of screening Compounds wherein target cells are coated onto a population of microbeads, and wherein each microbead is coated with several cells of the same cellular type and has an assay and an assay reporter associated with it. Each of the cell-coated microbeads are positioned in...

20060240417 - Agent controlling the apoptosis induction by p73: Elucidation of the interaction between p73 and ΔNp73 on the genetic and protein level, regulators for p73 or p53 apoptosis-inducing activity, a method of accelerating apoptosis of tumor cells by utilizing the regulators, p53 and p73 transactivation regulators comprising the p73 gene and ΔNp73 gene, nucleic acid pharmaceutical compositions comprising...

20060240467 - Analyte sampling element, analyte treatment device and analyte treatment method: In order to simplify the pretreatment such as collection of an analyte, treatment of the analyte, introduction of the analyte into a measurement system (reaction system) and dilution of the analyte and to improve operability in examinations using large-sized automatic analyzers and POCT devices, the present invention provides an analyte...

20060240418 - Canine gene microarrays: The present invention is based on the identification of novel canine nucleic acid sequences and the construction of canine microarrays containing a significant portion of the canine genome. The microarrays specifically hybridize to canine nucleic acid samples and may be used in drug screening and toxicity assays....

20060240422 - Caspase 10 as target for monitoring and treatment of diseases: The present invention relates to a method of monitoring and/or modulating disease-associated activatory procceses which are mediated by caspase-10 or caspase-10 isoforms....

20060240448 - Cell concentration and lysate clearance using paramagnetic particles: Methods are disclosed for using paramagnetic particles to concentrate or harvest cells. Methods are also disclosed for clearing a solution of disrupted biological material, such as a lysate of cells or a homogenate of mammalian tissue. Methods are also disclosed for using paramagnetic particles to isolate target nucleic acids, such...

20060240459 - Clckb mutation as a diagnostic and therapeutical target: The present invention relates to a method for diagnosing hypertension, and/or allergy, and/or hair loss, and/or liabilty for infection, of a human being, or a predisposition therefor; to a nucleic acid molecule coding for a human ClCKb protein comprising a genetic alteration at amino acid position 481 compared to the...

20060240453 - Clinically intelligent diagnostic devices and methods: The invention relates to the clinically intelligent design of diagnostic devices (such as microarrays) and methods of making and using such devices in differential diagnoses of specific clinical symptoms or sets of symptoms. In one aspect, the devices include various probes used to perform parallel screening of a number of...

20060240465 - Cng2b: a novel human cyclic nucleotide-gated ion channel: The invention provides isolated nucleic acid and amino acid sequences of CNG2B, antibodies to CNG2B, methods of detecting CNG2B, and methods of screening for modulators of cyclic nucleotide-gated ion channels using biologically active CNG2B. The invention further provides, in a computer system, a method of screening for mutations of human...

20060240451 - Compositions and methods employing 5' phosphate-dependent nucleic acid exonucleases: The present invention relates to compositions and methods employing 5′-phosphate-dependent nucleic acid exonucleases. In particular, the present invention provides kits and methods employing 5′-phosphate-dependent nucleic acid exonucleases for selective enrichment, isolation and amplification of a particular set of desired nucleic acid molecules from samples that also contain undesired nucleic acid...

20060240444 - Detection methods: A method of detecting one or multiple target molecules in a test sample....

20060240436 - Diagnostics and therapeutics for diseases associated with complement component 5a receptor (c5ar): The invention provides a human C5AR which is associated with the cardiovascular disorders, inflammatory diseases, gastrointestinal and liver diseases, cancer disorders, hematological disorders, respiratory diseases and urological disorders. The invention also provides assays for the identification of compounds useful in the treatment or prevention of cardiovascular disorders, inflammatory diseases, gastrointestinal...

20060240433 - Facls as modifiers of the rb pathway and methods of use: Human FACL genes are identified as modulators of the RB pathway, and thus are therapeutic targets for disorders associated with defective RB function. Methods for identifying modulators of RB, comprising screening for agents that modulate the activity of FACL are provided....

20060240458 - Flexible culture medium bag containing nutrient concentrate: Disclosed is a culture medium container such as a bag comprising a main compartment and a locus of containment that contains a nutrient concentrate until it is released at the time of use. The locus of containment may comprise separated compartments defined by frangible seals or seals comprising a water-reactive...

20060240452 - Flourecent quinacridone derivatives: The present invention relates to methods and compositions utilizing fluorescent quinacridone derivatives. In particular, the present invention relates to the use of fluorescent quinacridone derivatives for the labeling and detection of riucleic acids. The present invention thus provides improved compositions and methods for labeling biological molecules useful in the detection...

20060240470 - Gene causative of rothmund-thomson syndrome and gene product: The RecQ4 helicase gene, belonging to the RecQ helicase gene family, is revealed herein to be the causative gene of Rothmund-Thomson syndrome. The present inventors found out that it is possible to diagnose Rothmund-Thomson syndrome by detecting mutation of this gene. Further, they uncovered that it is possible to treat...

20060240426 - Gene expression in biological conditions: The present invention relates to a method of predicting the prognosis of a biological condition in animal tissue, wherein the expression of genes is examined and correlated to standards. The invention further relates to the treatment of the biological condition and. an assay for predicting the prognosis. In particular, the...

20060240441 - Gene expression profiles and methods of use: The present invention relates to gene expression profiles for lung cancer, microarrays comprising nucleic acid sequences representing gene expression profiles, and methods of using expression profiles and microarrays. The invention also provides methods and compositions for diagnostic assays for detecting cancer and therapeutic methods and compositions for treating cancer. The...

20060240425 - Genes and polypeptides relating to myeloid leukemia: The present application provides novel human genes RHBDF1 whose expression is markedly elevated in CML and AML compared to normal peripheral blood cell, and lung adenocarcinoma compared to normal lung cell. The genes and polypeptides encoded by the genes can be used, for example, in the diagnosis of a cell...

20060240450 - Genetic analysis for stratification of cancer risk: The present invention provides new methods for the assessment of cancer risk in the general population. These methods utilize particular alleles of two or more genes, in combination, to identify individuals with increased or decreased risk of cancer. Exemplified is risk assessment for breast cancer in women. In addition, personal...

20060240421 - Genetic polymorphisms sensitively predicting adverse drug reactions (adr) and drug efficacy: The invention provides diagnostic methods and kits including oligo and/or polynucleotides or derivatives, including as well antibodies determining whether a human subject is at risk of getting adverse drug reaction after statin therapy or whether the human subject is a high or low responder or a good a or bad...

20060240471 - Genetic test for the identification of carriers of complex vertebral malformations in cattle: Genetic markets for identifying bovine carriers of complex vertebral malformation (CVM) disease gene are described. The genetic markers, including the microsatellite markers, BM4129, INRAA003, BMS2790, ILSTS029, INRA123, BM220, HUJ246, BMS862, BMS937, BL1048, BMS2095 and BMS1266 and the bovine SLC35A3 gene, are located on bovine chromosome BTA3. The G/T polymorphism at...

20060240423 - Isolation and cloning of dna from uncultivated organisms: The present invention relates to a device for the isolation and/or purification of nucleic acid molecules suitable to bind and/or inactivate inhibitors of the activity of reagents or enzymes used for DNA manipulation and to separate a plurality of nucleic acid molecules with respect to their size. Moreover, the invention...

20060240432 - Lrp4/corin dopamine-producing neuron proliferation precursor cell marker: In neuron transplantation therapy, in terms of safety, it is preferable to use a cell population consisting only of a desired type of cells, and to use postmitotic neurons in consideration to avoid the risk of tumorigenesis. Moreover, greater therapeutic effects would be expected through the use of earlier progenitor...

20060240463 - Markers associated with the therapeutic efficacy of glatiramer acetate: The present invention is directed to methods and kits based, at least in part, on the identification of allele-specific responsiveness or non-responsiveness to glatiramer acetate for the treatment of immune disorders, such as relapsing-remitting multiple sclerosis. The allele-specific responsiveness or non-responsiveness is based on polymorphisms in the following genes, CTSS,...

20060240460 - Method for detecting methylated cpg islands: The present invention provides new and improved assay for detection of genomic methylated CpG islands. This new method is termed the methylated-CpG island recovery assay (MIRA). In accordance with one embodiment, MIRA comprises the steps of: (a) incubating genomic DNA fragments with a methylated CpG island binding protein in the...

20060240469 - Method for generating transcriptionally active dna fragments: A method for producing transcriptionally active DNA molecules, comprising (PCR) amplification of said DNA molecule in the presence of a first DNA fragment (F1), second DNA fragment (F2), first primer (P1), a second primer (P2), a third primer (P3), and a fourth primer (P4) wherein: F1 comprises a promoter sequence;...

20060240430 - Method for hybridisation of immobilized genomic dna: The present invention is directed to a novel method of efficiently hybridising probes onto immobilized genomic DNA and/or RNA comprising the steps of (a) providing intact genomic DNA and denaturing said intact genomic DNA; (b) immobilizing said denatured intact genomic DNA onto a matrix; said matrix comprising pore sizes within...

20060240427 - Method for the detection of pathogenic gram positive bacteria from the genera staphylococcus, enterococcus and streptococcus: The present invention is directed to a method for identification of a Gram positive pathogenic bacterium comprising an amplification step with at least a first set of amplification primers capable of amplifying a preselected nucleic acid sequence region from a first predetermined sub-group of pathogenic Gram positive bacteria, and a...

20060240419 - Method of detecting gene polymorphism: A method for detecting a genetic polymorphism(s), comprising creating oligonucleotide probes and/or oligonucleotide primers so that the probes and/or primers contain a polymorphic site(s) present in a gene encoding a receptor or a sequence complementary thereto or so that the polymorphic site(s) is/are contained in the amplified fragment when at...

20060240428 - Method of identifying disease-sensitivity gene and program and system to be used therefor: The object of this invention is to provide a method for efficiently identifying a disease susceptibility gene and, in particular, a disease susceptibility gene of a disease such as a multifactorial disease where a large number of genes are involved. This invention relates to a method for identifying a disease...

20060240468 - Method of reverse transcription: The present invention relates to reverse transcription of RNA, and in particular to reverse transcription by thermostable DNA polymerases. Thermoactinomyces vulgaris and Bacillus stearothermophilus possess reverse transcriptase activity in the presence of magnesium or manganese ions. Methods, compositions, and kits for reverse transcription and RT-PCR are also provided....

20060240429 - Method of screening compound inhibiting enzymatic activity of gwt1 gene product: The present invention enables screening for compounds that inhibit the transport of GPI-anchored proteins to fungal cell walls, using a simple assay for transacylation to GlcN-PI using membrane fraction expressing GWT1 protein. New antifungal agents can be created that inhibit the synthesis of fungal cell walls and also inhibit adhesion...

20060240449 - Methods and compositions for preparation of biological samples: Methods and compositions for preparation of biological samples are disclosed. The methods include a prelysis step and a lysis step to make the cellular DNA available for further processing, amplification or analysis. The prelysis step includes the addition of a prelysis reagent to the cells. The prelysis reagent may include...

20060240440 - Methods and compositions for the correlation of single nucleotide polymorphisms in the vitamin k epoxide reductase gene and warfarin dosage: The present invention provides a method of identifying a human subject having increased or decreased sensitivity to warfarin, comprising detecting in the subject the presence of a single nucleotide polymorphism in the VKOR gene, wherein the single nucleotide polymorphism is correlated with increased or decreased sensitivity to warfarin, thereby identifying...

20060240442 - Methods and oligonucleotides for the detection of salmonella sp., e coli 0157:h7, and listeria monocytogenes: A method for detecting a Salmonella species, E. coli 0157:H7, or Listeria monocytogenes is disclosed. The method involves amplifying a genomic nucleotide sequence of a corresponding species and detecting the amplification product. Various primers and probes that can be used in the method are also disclosed. In one embodiment, the...

20060240462 - Methods for amplification and detection of nucleic acids: Provided herein are methods for the combined amplification and detection of one or a plurality of target nucleic acid molecules. The methods encompass the use of an antisense strand of a catalytic nucleic acid in a primer for amplification such that an amplicon produced thereby includes an active catalytic nucleic...

20060240457 - Methods for characterizing agonists and partial agonists of target molecules: In one aspect, the present invention provides methods of determining whether an agent is more like a partial agonist of a target molecule than a full agonist of the same target molecule. In another aspect, the present invention provides methods to select a candidate compound that may reduce blood plasma...

20060240461 - Methods for detecting nemaline myopathy: The present invention provides identification of a mutation related to the nebulin gene (NEB), which can cause nemaline myopathy (NM). The present invention also provides a method for detecting such mutation in a human cell or individual, such as an NM-derived cell or an individual suffering from NM. The present...

20060240443 - Microarray-based single nucleotide polymorphism, sequencing, and gene expression assay method: There is disclosed a microarray-based single nucleotide polymorphism, sequencing, and gene expression assay method. Specifically, there is disclosed a method using a microarray device wherein a plurality of hybridized structures is formed by contacting the microarray under a hybridizing condition to a hybridizing solution comprising a plurality of tagged targets...

20060240454 - Mmethod of analyzing breast cancer susceptibility and resistance: A method of analyzing breast cancer susceptibility or resistance, comprising the steps of obtaining a DNA sample from a patient, genotyping the sample for the presence of the minor allele of single nucleotide polymorphisms rs6476643 or rs2182317, and correlating the identity of the allele with risk assessment data is disclosed....

20060240439 - Modified polymerases for improved incorporation of nucleotide analogues: The invention relates to modified polymerase enzymes which exhibit improved incorporation of nucleotide analogues bearing substituents at the 3′ position of the sugar moiety that are larger in size than the naturally occurring 3′ hydroxyl group. Also described are methods of using the polymerases to incorporate nucleotides into polynucleotides, particularly...

20060240416 - Multianalyte molecular analysis using application-specific random particle arrays: The present invention provides a method for the generation of novel libraries of encoded magnetic particles from sub-libraries of by the generation of novel sub-libraries of magnetic nanoparticles and encoded particles. The sub-libraries are functionalized on demand are useful in the formation of arrays. The present invention is especially useful...

20060240445 - Multiplex snp typing by bioluminometric assay coupled with terminator incorporation: The present invention provides a method for analyzing a nucleotide sequence comprising the steps of: carrying out complementary strand synthesis by adding at least one of four kinds of ddNTP corresponding to nucleotides A, G, T, and C, or derivatives thereof to a reaction vessel containing a nucleic acid sample...

20060240456 - Nanosized biological container and manufacture thereof: Nanosized biological containers that are “ghosts” of viruses for which capsids are independent of their endogenous viral nucleic acid cores, provide nano-particles of uniform size, and known numbers of sites for attachments of ligands. These containers can be filled with a fluorescent, magnetic, x-ray absorbent, nucleotide components or a radioactive...

20060240464 - Novel 27877, 18080, 14081, 32140, 50352, 16658, 14223, 16002, 50566, 65552, and 65577 molecules and uses therefor: The invention provides isolated nucleic acids molecules, designated 27877, 18080, 14081, 32140, 50352, 16658, 14223, 16002, 50566, 65552 and 65577 nucleic acid molecules. The invention also provides antisense nucleic acid molecules, recombinant expression vectors containing 27877, 18080, 14081, 32140, 50352, 16658, 14223, 16002, 50566, 65552 and 65577 nucleic acid molecules,...

20060240446 - Novel human cytomegalovirus dna sequences: Provided are novel Toledo and Towne human cytomegalovirus DNA sequences (HCMV) and proteins encoded thereby. The sequences are useful in methods and compositions for detecting HCMV infections and in immunogenic compositions for Preventing HCMV infections....

20060240420 - Nucleic acid amplification method: A method for increasing the number of polynucleotides containing sequences corresponding to a mRNA species present in a sample, comprises the steps of: (i) reverse transcription of the RNA species using a heeled 5′-amplification primer (FAP-RAND) and a heeled 3′-amplification primer (TAP-RT), wherein each primer sequence is unique, and either...

20060240447 - Oligonucleotide sequences for the detection of ricin gene and toxin: Novel oligonucleotides useful as primers and probes in the detection and identification of ricin toxin gene and gene product are provided. A method and kit for detecting and identifying a nucleic acid from Ricinus communes are also provided....

20060240431 - Oligonucletide guided analysis of gene expression: The present invention relate to methods and compositions for simultaneously analyzing multiple different polynucleotides of a nucleic acid sample. The subject methods and compositions may also be applied to analyze or identify single polynucleotide; however, the subject methods and compositions are particularly useful for analyzing large diverse populations of polynucleotides....

20060240455 - Oxazine derivatives: Oxazine derivatives with which a broad spectrum of material to be studied can be labelled and identified by means of fluorescence techniques are described....

20060240437 - Predictors of renal disease: The invention includes diagnostic, predictive, prognostic and monitoring methods and reagents for loss of renal function, e.g., diabetic nephropathy and end stage renal disease (ESRD)....

20060240466 - Prevention of type 1 diabetes and other non-polio enterovirus diseases: Live virus vaccines comprise attenuated viruses, while other vaccines comprise killed viruses or parts thereof. It has now been found that the immune response induced by oral poliovirus vaccine (OPV), which is a live vaccine, is cross-reactive with non-polio enteroviruses. OPV is therefore useful in the prevention of non-polio enterovirus...

20060240435 - Process for producing macromolecule-identifying polymer: The methods for producing macromolecule identifying polymers according to the present invention comprise the steps of polymerizing a starting monomer in an aqueous solution in the presence of a macromolecule, a crosslinking agent, and a radical polymerization initiator to produce a polymer containing the macromolecule in its interior; and removing...

20060240424 - Regulation of human p2y15 g protein-coupled receptor: Reagents which regulate human P2Y15 G protein-coupled receptor can play a role in preventing, ameliorating, or correcting bronchoconstriction or inflammation in diseases such as allergies including but not limited to asthma. Further, such reagents is useful to treat diseases of kidney function or disease related to mast cell....

20060240434 - Standardized and optimized real-time quantitative reverse transcriptase polymerase chain reaction method for detection of mrd in leukemia: The invention relates to in a real-time quantitative reverse transcriptase polymerase chain reaction (RQ-PCR) method for minimal residual disease detection in leukemic patients through amplification of a fusion gene transcript, the improvement comprising: (i) selecting amplifiable and qualified patient samples for subsequent analysis; (ii) defining optimal conditions for performing the...

20060240438 - Surface of base material being inhibited in non-specific adsorption: A substrate surface on which either a substance to detect analyte or an analyte per se is immobilized, which surface is formed by a treatment of substrate surface with a liquid which contains uncrosslinked polymer based on polyethylene glycol chain segment, said treatment conducted either simultaneously with the immobilization of...

20060240478 - Biosensor: One object of the present invention is to provide a biosensor and a production method therefor, by which hydrogel that enables immobilization of a physiologically active substance can be conveniently produced using safe raw materials. The present invention provides a biosensor which comprises a substrate having a metal layer on...

20060240476 - Construct comprising recognition domain of antibody against von willebrand factor-specific cleaving enzyme: The present invention provides an epitope recognized by an antibody (hereinafter, also referred to as an anti-ADAMTS-13 antibody) against a cleaving protease (hereinafter, also referred to as ADAMTS-13) specific to von Willebrand factor (hereinafter, also referred to as vWF), and a polypeptide comprising the epitope region. The present invention also...

20060240475 - Diagnostic method for celiac sprue: Detection of toxic gluten oligopeptides refractory to digestion and antibodies and T cells responsive thereto can be used to diagnose Celiac Sprue....

20060240479 - Fluorescent label: The present invention relates to a fluorescent label characterized by containing a hydrophilic polymer having an anionic group, a polyether derivative, and a phosphor, in which the phosphor is bound to the hydrophilic polymer via the polyether derivative, and also relates to a fluorescently labeled recognition substance labeled with the...

20060240481 - Melanocortin metallopeptode combinatorial libraries and applications: Melanocortin receptor-specific peptide combinatorial libraries for forming metallopeptides and metallopeptide combinatorial libraries are provided for use in biological, pharmaceutical and related applications. The combinatorial libraries are constructed of peptides constructed from naturally occurring amino acids, stereoisomers and modifications of such amino acids, non-protein amino acids, post-translationally modified amino acids, enzymatically...

20060240477 - Method for the production of near net-shaped metallic and/or ceramic parts: The present invention relates to a peptide comprising a symmetrical dimethylated arginine, and constitute an immunologic determinant of antibodies present in sera from patients with systemic lupus erythematosus (SLE), and wherein the methylation is a prerequisite for reacting with said antibodies. The invention also relates to the use of said...

20060240482 - Methods of mhc class ii epitope mapping, detection of autoimmune t cells and antigens, and autoimmune treatment: The present invention provides of using multimeric MHC class II/peptide complexes. In one aspect, methods provided for identifying MHC class II-restricted immune epitopes of a predetermined polypeptide antigen. Methods for identifying an immunostimulatory epitope for a predetermined polypeptide antigen are provided. In a related aspect, methods for screening a therapeutic...

20060240474 - Peptides able to switch between two structural states and their use: The present invention relates to peptides that switch between 2 structural states in response to the presence or absence of a stimulus. The present invention also relates to the use of the peptides of the present invention in an assay to detect the presence or absence of a stimulus. The...

20060240472 - Pigment protein: An object of the present invention is to provide a novel chromoprotein derived from Cnidopus japonicus. The present invention provides a chromoprotein derived from Cnidopus japonicus having the following properties: (1) the absorption maximum wavelength is 610 nm, and fluorescence is not emitted; (2) the molar absorption coefficient is 66,700...

20060240473 - Saccharide libraries: Novel methodologies for producing saccharide libraries are provided as well as the libraries themselves....

20060240480 - Use of gfap for identification of intracerebral hemorrhage: The present invention relates to the use of glial fibrillary acidic protein (GFAP) as a diagnostic marker for intracerebral hemorrhage. The invention especially relates to methods for the early detection of intracerebral hemorrhage. Such early and rapid detection can be performed rapidly e.g. by a test strip format assay. GFAP...

20060240486 - Immunoprecipitation-based assay for predicting in vivo efficacy of beta-amyloid antibodies: In various aspects, the present invention provides methods and kits for predicting the therapeutic efficacy of an immunological reagent, identifying an immunological reagent having therapeutic efficacy, or both, for the treatment of an amyloidogenic disorder by comparing the amount of Aβ monomer in an Aβ preparation which binds to the...

20060240483 - Method and kit for monitoring recruitment of proteins to the intracellular domain of a receptor in intact cells: The present invention relates to an immunological method and kit for monitoring recruitment of a protein to the intracellular domain of a receptor in intact cells....

20060240485 - Method of monitoring immunotherapy: The present invention relates to a method of monitoring an immunotherapy against amyloidosis and other diseases characterized by the deposition of abnormal protein aggregates. More specifically, it relates to a method of evaluating an immunotherapy against Alzheimer's disease, based on a novel assay that is characterized by scoring immunoreactivity levels...

20060240484 - Protein for diagnosing diabetic retinopathy: The present invention relates to material for diagnosing Diabetic retinopathy. More particularly, the present invention relates to Immunoglobulin A protein for diagnosing Diabetic retinopathy, kit for diagnosing Diabetic retinopathy comprising antibody against the protein and method for diagnosing Diabetic retinopathy. The present invention can be used as diagnosing Diabetic retinopathy....

20060240490 - Methods and compositions for identifying target cell cytolytic lymphocytes in a sample: Methods and compositions for identifying target cell cytolytic lymphocytes, e.g., T-cells, such as neoplastic cell cytolytic T-cells, in a subject are provided. In practicing the subject methods, the sample is contacted with a target cell stimulator, e.g., a neoplastic cell, and a detectably labeled granule membrane protein specific binding agent....

20060240491 - Proteins involved in cytoadhesion of plasmodium falciparum ring-stage-infected erythrocytes, antibodies which bind to the proteins, and methods for detecting infection, stage of infection and vaccines for protecting against infection: The present invention provides hybridomas and antibodies which specifically react with native P. falciparum at a surface of ring stage infected erythrocytes and uses thereof....

20060240492 - Carbon nanotube based immunosensors and methods of making and using: An immunoassay device comprises a plurality of carbon nanotubes having a first end and a second end, wherein the nanotubes are aligned substantially parallel relative to one another; a substrate responsive to an electrochemical signal, the substrate being attached to the first end of at least a portion of the...

20060240493 - Cca1 as an antifungal target: The invention provides ATP(CTP): tRNA nucleotidyltransferase (CCA1) as a novel antifungal target, screening methods for CCA1 inhibitors and their use as antifungal compounds, pharmaceutical compositions containing them and their use in medicine. Specifically in the treatment of an individual susceptible to or suffering from an anti-fungal infection. In particular the...

20060240494 - Biocidal molecules, macromolecular targets and methods of production and use: A method for identifying a compound that has a biocidal effect against a selected organism involves screening from among known or unknown peptide or non-peptide molecules, a test molecule that binds selectively to a target sequence of a multi-helical lid of a heat shock protein of the organism. The binding...

20060240489 - Carbodithioate ligands for nanotechnology and biosensing applications: The present invention is directed to methods and products related to carbodithioate ligands bonded to surfaces. The invention is further directed to molecular and biomolecular sensing methods based on analyte recognition by carbodithioate ligands bonded to surfaces....

20060240488 - Detection of an immune response to gdf-8 modulating agents: This disclosure provides methods for the detection of antibodies to a GDF-8 modulating agent such as, e.g., MYO-029, in a biological sample. Methods to detect an immune response to a GDF-8 modulating agent are also included. In particular, methods to assess an immune response in animals, including humans, to a...

20060240487 - Detection of gdf-8 modulating agents: Methods to detect GDF-8 modulating agents in animals, including humans, are provided herein, including methods to detect the presence of exogenous GDF-8 modulating agent such as a GDF-8 inhibitor in a biological sample. In particular, methods to assess the presence and/or quantity of a GDF-8 modulating agent in a biological...

20060240497 - Assaying apparatus, kit, and method for lipids and associated enzymes: Lipid assay method, kit, and apparatus involving exposure of a protein, having a lipid recognition motif that interacts with a target lipid and a competing lipid, to a solution containing the competing lipid, and determining whether the target lipid is present in the solution. The target lipid has a stronger...

20060240496 - Immunogens, derivatives and immunoassay for ethyl glucuronide: Ethyl glucuronide (“EtG”) analogs can be prepared for use as immunodiagnostic reagents and in immunodiagnostic protocols. The EtG analogs can be in the form of EtG-based immunogens, and EtG-based antigens. The EtG-based immunogens can be used for preparing anti-EtG antibodies, which can be used in immunoassays. Accordingly, improved immunoassay techniques...

20060240495 - Non-invasive assessment of intra-amniotic environment: Assessing fetal health and maturity, and the integrity and health of the amnion, is effected via protein profiling of vaginal fluid, thereby to identify various abnormal conditions during pregnancy. This approach also provides a means for gauging the duration and/or magnitude of intraamniotic/fetal inflammation that occurs during pregnancy....

20060240498 - Devices and methods for detecting amniotic fluid in vaginal secretions: The present invention relates to a diagnostic method for the detection of small quantities of amniotic fluid in the vagina. More specifically, the invention relates to the detection of PAMG-1 in the vagina using anti-PAMG-1 antibodies....

20060240499 - Methods for determining phosphoryltransferase activity using adenosine 5'-triphosphate (atp)-gamma-s: This invention relates to screening assay methods that can be applied to broad members of the protein kinase gene family and are useful in the detection and evaluation of kinase inhibitors....

20060240500 - Diagnosis of kidney damage and protection against same: Various nucleic acids and proteins have been identified by differential hybridization methods as useful as markers for diagnosing kidney damage. The identified marker proteins include (I) androgen related protein, SON protein, FUSE binding Protein 1, claudin10, heat shock protein, phospho triesterase related protein, ubiquitin protein ligase Nedd-4, and Ac39/physophilin, and...

20060240501 - Defructosylation method: The present invention is directed to a defructosylation enzyme originating from a plant, a method of defructosylating a fructosylated peptide or protein through use of the enzyme, and a method of measuring a fructosylated peptide or protein....

20060240502 - Gtp cyclohydrolase ii as a target for fungicides: The present invention relates to the identification of fungal GTP cyclohydrolase II as a target for fungicides, to a method for identifying antifungal agents based on fungal GTP cyclohydrolase II, and also to the use of compounds identified as fungicides via the abovementioned method....

20060240503 - Protease detection assay: The invention provides methods and compounds for detecting protease activity in a sample solution comprising contacting the sample solution with a protease substrate labelled with an electrochemically active marker, providing conditions under which any protease which may be present in the sample may degrade the protease substrate and electrochemically determining...

20060240504 - Evaluating bacterial lethality of containerized food production: Procedures and means for evaluating effectiveness of bacterial-lethality, following batch-processed containerized food production operations and aseptic-flow food-production operations as containerized in aseptic-containers, in preparing for non-refrigerated marketing are described. The evaluations significantly expedite determining whether thermally-processed containerized food-production is safe for non-refrigerated marketing. The presence or absence of live spore-forming...

20060240505 - Identification of targets of antimicrobial compounds: A method of determining the cellular or genetic target of an antimicrobial compound includes cloning of a bacterial gene into an expression vector with an inducible promoter and determining whether increasing expression of the cloned gene in the cell will result in resistance to an antimicrobial compound. Also, the method...

20060240507 - Cationic anti-microbial peptides and methods of use thereof: Described herein are methods of detecting a wound infection and for detecting the presence or absence of microorganisms, for example, wound pathogens in a sample, by contacting a sample with a cationic anti-microbial peptide that is degradable by an enzyme produced and/or secreted by a microorganism, and detecting degradation or...

20060240506 - Method for isolating and culturing unculturable microorganisms: The invention provides a method for isolating and culturing a previously unculturable microorganism, which comprises: (i) collecting a sample from an environmental source; (ii) counting/estimating the number of microorganisms in the sample; (iii) diluting the sample in an appropriate medium; (iv) adding a gelating agent such as to entrap one...

20060240508 - Preparation of 7-dehydrocholesterol and/or the biosynthetic intermediates and/or secondary products thereof in transgenic organisms: The present invention relates to a method for preparing 7-dehydrocholesterol and/or the biosynthetic intermediates and/or secondary products thereof by culturing organisms, in particular yeasts. Furthermore, the invention relates to the preparation of the nucleic acid constructs required for preparing the genetically modified organisms and to said genetically modified organisms, in...

20060240514 - Animal product free media and processes for obtaining a botulinum toxin: Media and processes for the fermentation of Clostridium botulinum and obtaining a botulinum toxin for use in formulating botulinum toxin pharmaceutical compositions. The growth media can contain significantly reduced levels of meat or dairy by-products using non-animal based products to replace the animal-derived products. Preferably, the media used are substantially...

20060240513 - Cultures of e1-immortalized cells and processes for culturing the same to increase product yields therefrom: The invention provides processes for culturing cells derived from embryonic retinoblast cells immortalized by adenovirus E1 sequences, preferably PER.C6® cells, to improve product yields from such cells. Feed strategies for such cells and cultures with very high cell densities are provided, resulting in high yields of products, such as recombinant...

20060240521 - Dna sequences for regulating transcription: The invention relates to a DNA sequences for regulating transcription of a structural gene encoding a polypeptide in a eukaryotic host cell comprising (a) a first DNA sequence to which RNA polymerase binds which DNA sequence comprises a mRNA initiation site; and further (b) one or more DNA sequence(s) to...

20060240523 - Endogenous and non-endogenous versions of human g protein-coupled receptors: The invention disclosed in this patent document relates to transmembrane receptors, more particularly to a human G protein-coupled receptor and to mutated (non-endogenous) versions of the human GPCRs for evidence of constitutive activity....

20060240516 - Interacting polypeptide comprising a heptapeptide pattern and a cell penetration domain: The invention relates to an interacting polypeptide consisting of or comprising a heptapeptide pattern of sequence X1X2X3X4X5X6X7 and a transduction domain, characterized in that it is a chimera polypeptide, the amino acid X7 is located between 5 and 35 amino acids of the C-terminal end of said polypeptide, and that...

20060240510 - Method for designing peptides: The present invention relates to genetic engineering and; in specific, to design, generation, and modification of recombinant peptides using a combination of phage display and intein-mediated protein cleavage reaction....

20060240509 - Myrothecium sp transformation and expression system: The present invention is related to a transformation and an expression system in which Myrothecium sp. host cells are used to express homologous or heterologous proteins or are used to genetically engineer metabolic pathways....

20060240517 - Novel receptor nucleic acids and polypeptides: Disclosed are nucleic acids encoding BAFF-R polypeptides, as well as antibodies to BAFF-R polypeptides and pharmaceutical compositions including the same. Methods of treating tumorigenic and autoimmune conditions using the nucleic acids, polypeptides, antibodies and pharmaceutical compositions of this invention are also provided....

20060240518 - Novel receptor nucleic acids and polypeptides: Disclosed are nucleic acids encoding BAFF-R polypeptides, as well as antibodies to BAFF-R polypeptides and pharmaceutical compositions including the same. Methods of treating tumorigenic and autoimmune conditions using the nucleic acids, polypeptides, antibodies and pharmaceutical compositions of this invention are also provided....

20060240519 - Novel receptor nucleic acids and polypeptides: Disclosed are nucleic acids encoding BAFF-R polypeptides, as well as antibodies to BAFF-R polypeptides and pharmaceutical compositions including the same. Methods of treating tumorigenic and autoimmune conditions using the nucleic acids, polypeptides, antibodies and pharmaceutical compositions of this invention are also provided....

20060240520 - Novel receptor nucleic acids and polypeptides: Disclosed are nucleic acids encoding BAFF-R polypeptides, as well as antibodies to BAFF-R polypeptides and pharmaceutical compositions including the same. Methods of treating tumorigenic and autoimmune conditions using the nucleic acids, polypeptides, antibodies and pharmaceutical compositions of this invention are also provided....

20060240522 - Process for bacterial production of polypeptides: Refractile particles containing a heterologous polypeptide as an insoluble aggregate are recovered from bacterial periplasm. The process involves culturing bacterial cells so as to express nucleic acid encoding phage lysozyme and nucleic acid encoding the heterologous polypeptide under separate promoters, disrupting the cells mechanically to release the phage lysozyme so...

20060240512 - Pt32 sperm protein, sperm c-yes, oocyte cytoplasmic c-yes, and uses thereof: The perinuclear theca 32 (PT32) protein is disclosed and shown to interact with tyrosine kinase c-Yes. PT32, c-Yes, fragments thereof, and molecules that bind thereto can be used in methods of enhancing fertility, treating or diagnosing diminished fertility and abnormal spermiogenesis, in providing contraception, and in identifying contraceptive and fertility-enhancing...

20060240511 - Purification of her-2 variants: The present invention provides for a novel method for purification of EGFR family proteins obtained from cultures of insect cells. The process comprises subsequent steps of a) diafiltration and exchange of culture medium with buffer, b) immobilized metal affinity chromatography (IMAC), c) size exclusion chromatography (SEC), and d) anion exchange...

20060240515 - Soluble fragments of the sars-cov spike glycoprotein: The invention relates to the spike protein from the virus (SARS-CoV) that is etiologically linked to severe acute respiratory syndrome (SARS); polypeptides and peptide fragments of the spike protein; nucleic acid segments and constructs that encode the spike protein, polypeptides and peptide fragments of the spike protein, and coupled proteins...

20060240524 - Factor vii or viia - like molecules: Conjugates of Factor VII (FVII) and Factor VIIa (FVIIA) are provided, as are methods for preparing them. Methods for producing novel polypeptides contributing to the production of such conjugates are provided. Methods of treatment by administering a FVII or FVIIa conjugate are provided....

20060240525 - Factor vii or viia - like molecules: Conjugates of Factor VII (FVII) and Factor VIIa (FVIIA) are provided, as are methods for preparing them. Methods for producing novel polypeptides contributing to the production of such conjugates are provided. Methods of treatment by administering a FVII or FVIIa conjugate are provided....

20060240526 - Fvii or fviia gla domain variants: Gla domain variants of human Factor VII or human Factor VIIa, comprising 1-15 amino acid modifications relative to human Factor VII or human Factor VIIa, wherein a hydrophobic amino acid residue has been introduced by substitution in position 34; or having an amino acid substitution in position 36; and use...

20060240527 - Process for producing fine self-aggregates: a

20060240528 - Polyketides and their synthesis: A polyketide synthase (“PKS”) of Type I is a complex multienzyme including a loading domain linked to a multiplicity of extension domains. The first extension module receives an acyl starter unit from the loading domain and each extension module adds a further ketide unit which may undergo processing (e.g. reduction)....

20060240529 - Process for the preparation of fludarabine phosphate from 2-fluoroadenine and fludarabine phosphate salts with amines or ammonia: The invention provides a process for the preparation of fludarabine phosphate from 2-fluoroadenine and 9-β-D-arabinofuranosyl-uracil using Enterobacter aerogenes (EBA). 2-Fluoroadenine is reacted with 9-β-D-arabinosyl-uracile in a water solution at pH=7 in the presence of EBA cell paste, to yield fludarabine. Fludarabine is then treated with acetic anhydride and the resulting...

20060240530 - Methods and compositions for infectious cdna of sars coronavirus: The present invention provides a cDNA of a severe acute respiratory syndrome (SARS) coronavirus, recombinant SARS coronavirus vectors, and SARS coronavirus replicon particles. Also provided are methods of making the compositions of this invention and methods of using the compositions as immunogens and/or vaccines and/or to express heterologous nucleic acids....

20060240531 - Processes for producing isomaltose and isomaltitol and use thereof: The present invention aims to provide a novel process for producing isomaltose and isomaltitol, and uses thereof, and it solves the object by establishing a process for producing isomaltose comprising a step of contacting a saccharide, having the α-1,4 glucosidic linkage as the linkage of non-reducing end and a glucose...

20060240532 - Dna encoding hydantoinase, dna encoding n-carbamyl-l-amino acid hydrolase, recombinant dna, transformed cell, method of producing protein and method of producing optically active amino acid: The present invention provides a method of producing optically active amino acids from 5-substituted hydantoin by isolating a hydantoinase gene and an N-carbamyl-L-amino acid hydrolase gene involved in an ability to convert 5-substituted hydantoin or N-carbamylamino acid into optically active amino acids from a microorganism of the genus Microbacterium having...

20060240533 - Assembly for the stabilisation of vertebral bodies of the spine: An assembly for the stabilisation of vertebral bodies (12, 14) of the spine is described. It comprises a pair of pedicle screws (20) each having a threaded shaft (20a) with a tapering first end (20b) for introduction into a vertebral body and a head portion (20c) with a second end...

20060240534 - Process for producing scyllo-inositol: It is intended to provide a novel NAD+-independent myo-inositol 2-dehydrogenase which converts myo-inositol into scyllo-inosose in the absence of NAD+; a novel enzyme scyllo-inositol dehydrogenase which stereospecifically reduces scyllo-inosose into scyllo-inositol in the presence of NADH or NADPH; and a novel microorganism which belongs to the genus Acetobacter or Burkholderia...

20060240535 - Carbonyl reductase from lactococcus lactis: Compositions and methods of using a carbonyl reductase from Lactococcus lactis (LLCR or LL-CR) are provided. One aspect provides an isolated polypeptide from Lactococcus lactis, wherein the polypeptide is an (R)-specific carbonyl reductase or a fragment thereof and reduces acetophenone or alph,beta-unsaturated carbonyl compound to form a reduced R enantiomeric...

20060240536 - Fermentation broth extraction of k-252a: Methods for separating K-252a from the cell mass of its parent fermentation broth are disclosed....

20060240537 - Plants with increased phosphorous uptake: The invention provides plant acid phosphatase coding sequences. Also provided are constructs comprising these sequences, plants transformed therewith and methods of use thereof. In certain aspects of the invention, transgenic plants are provided exhibiting improved phosphorous utilization....

20060240538 - Cell separation method using alumina: A method of separating cells or viruses using alumina is provided. The method includes: contacting a solution containing cells or viruses with alumina; and washing the alumina having cells or viruses bound thereto. According to the method, the separation efficiency of cells or viruses can be increased, the detection limit...

20060240539 - Texturizing lactic bacteria: The invention relates to a novel strain of lactic bacteria comprising at least one sequence selected from a group consisting of nucleotide sequences SEQ ID No. 1, SEQ ID No. 2, SEQ ID No. 3, SEQ ID No. 4, SEQ ID No. 5, SEQ ID No. 6, SEQ ID No....

20060240540 - Biosensor, biosensor chip, and biosensor device: A biosensor device includes a biosensor having a plurality of biosensor sections each including a working pole and a counter pole. The biosensor device further includes a singal processing circuit for measuring a current flowing from the working pole when different voltages are applied between the working poles and the...

20060240541 - Lateral flow assay systems and methods: In one aspect, a diagnostic test system includes a housing, a reader, and a data analyzer. The housing includes a port for receiving a test strip. The reader obtains separable light intensity measurements from localized regions of an area of the detection zone exposed for optical inspection, wherein each of...

20060240542 - Substrate support lift mechanism: An apparatus for positioning a substrate support within a processing chamber is provided. In one embodiment, an apparatus for positioning a substrate support includes a yoke comprising a curved surface with a first slot formed therethrough, a base comprising a first surface adapted to support the substrate support and a...

20060240543 - Microwell arrays with nanoholes: A device for conducting parallel analysis or manipulation of multiple cells or biomolecules is disclosed. In one embodiment, the device comprises a silicon chip with a microwell, and at least one membrane suspended at the bottom opening of the microwell. The suspended portion of the membrane defines a nanohole that...

20060240544 - Bioreactor for growing fungus, plant cell, tissue, organ, hairy roots and plantlet: A bioreactor includes a nutrient reservoir, a rotatable culture bed, at least one culture medium, a mist generator and a mist delivery pipe. The nutrient reservoir stores therein a nutrient solution required for the fungus, plant cell, tissue, organ, hairy roots and plantlet. The rotatable culture bed is rotated at...

20060240545 - Flow cell and a reactor for reactions between fixed reacting substances and liquid reacting substances, and a method for operating the reactor: A flow cell having a first plate having a continuous groove formed as a passage on one surface of the plate and having gas/liquid inlet/outlet portions at both the ends of the continuous groove, and a second plate to be kept in contact with said one surface of the first...

20060240546 - Tube ports and related container systems: A tube port includes a tubular first stem having an interior surface and an exterior surface extending between a first end and a longitudinally spaced apart second end. The interior surface bounds a passage that longitudinally extends through the first stem. The tube port also includes a flange encircling and...

20060240547 - Linear and membrane-like biodevices and bioreactors: A linear biodevice is provided with fibers that have a curved portion in its cross section approximately perpendicular to the longitudinal direction and approximately spherical adhesive cells that adhere to the periphery of the fiber. A membrane-like biodevice is provided with a sheet that has an opening and approximately spherical...

20060240548 - Materials for constructing cell-chips, cell-chip covers, cell-chips coats, processed cell-chips and uses thereof: A chip-device is provided for holding living cells in a plurality of wells disposed on the surface of a carrier, characterized in that the wells are configures to influence the proliferation of living cells in the wells, for example by changing the size of the wells, delaying or inhibiting cell-proliferation,...

20060240549 - Lockable cell growth chamber: A lockable cell growth chamber is disclosed wherein the lid and base of the dish are provided with at least two pairs of locking members, each comprising a notched docking member and a docking member-engaging tab....

20060240550 - Three-dimensional cell to tissue development process: The present invention relates to an improved three-dimensional cell to tissue development process using a specific time varying electromagnetic force, pulsed, square wave, with minimum fluid shear stress, freedom for 3-dimensional spatial orientation of the suspended particles and localization of particles with differing or similar sedimentation properties in a similar...

20060240551 - Neutralizing monoclonal antibodies against severe acute respiratory syndrome-associated coronavirus: The present invention provides an isolated antibody capable of binding to the receptor-binding domain of the spike protein of the severe acute respiratory syndrome-associated coronavirus (SARS-CoV) so as to competitively inhibit the binding of the SARS-CoV to host cells. These mAbs or substances can be used: 1) as passive-immunizing agents...

20060240552 - Anterior ocular-associated cell sheet, three-dimensional construct and process for producing the same: An anterior ocular segment related cell sheet or three-dimensional structure that have only a few structural defects as they have been recovered retaining the intercellular desmosome structure and the basement membrane-like protein between cell and substrate. The anterior ocular segment related cell sheet or three-dimensional structure is produced by a...

20060240553 - Cultures of human cns neural stem cells: The invention provides a cell culture including proliferating human neural stem cells with a doubling rate faster than thirty days. The invention also provides a cell culture media for proliferating mammalian neural cells including a standard defined culture medium, a carbohydrate source, a buffer, a source of hormones, one or...

20060240554 - Lipid nanoparticle based compositions and methods for the delivery of biologically active molecules: The present invention relates to novel cationic lipids, transfection agents, microparticles, nanoparticles, and short interfering nucleic acid (siNA) molecules. The invention also features compositions, and methods of use for the study, diagnosis, and treatment of traits, diseases and conditions that respond to the modulation of gene expression and/or activity in...

20060240555 - Fibrin cell supports and method of use thereof: The present invention relates to fibrin cell supports for cell cultures formed by the mixture of plasma proteins including fibrinogen and thrombin. The fibrin cell supports are preferably used for preparing a culture of cells such as keratinocytes, recovering the culture in the form of a reconstituted tissue, and transporting...

20060240556 - Use of rna interference for the creation of lineage specific es and other undifferentiated cells and production of differentiated cells in vitro by co-culture: Methods for making human ES cells and human differentiated cells and tissues for transplantation are described, whereby the cells and tissues are created following somatic cell nuclear transfer. The nuclear transfer donor is genetically modified prior to nuclear transfer such that cells of at least one developmental lineage are de-differentiated,...

20060234203 - Methods, compositions and devices utilizing stinging cells/capsules for conditioning a tissue prior to delivery of an active agent: Methods, compositions and devices utilizing stinging cells/capsules for conditioning a tissue prior to delivery of a pharmaceutical agent are described....

20060234204 - Method for vitrification of a biological specimen: The present invention relates to a method of vitrification of a biological specimen. According to the method of the present invention, a biological specimen is directly exposed to a freezing material. Upon exposure to the freezing material, the biological specimen undergoes vitrification. The biological specimen which has undergone vitrification may...

20060234207 - Device for evaluating in vitro cell migration under flow conditions, and methods of use thereof: The current invention is directed towards a device that useful for studying cell migration. It is preferred that the device is configured to study stem cell, particularly hematopoetic stem/precursor cell homing. The device allows for detailed analysis of the various phases of the multiphase homing process. There are also provided...

20060234206 - Fluorescent probes for biological studies: e

20060234205 - In vitro test system for predicting patient tolerability of therapeutic agents: Methods for predicting patient tolerability to therapeutic agents, such as cytokines, lymphokines and immunotoxins, are disclosed. The methods utilize an in vitro model of vascular leak syndrome (VLS) to assess the effect of the agent in question on the permeability of large proteins across confluent monolayers of endothelial cells (EC)....

20060234210 - Filtration device and method for removing selected materials from biological fluids: A filter and method for removing selected materials from a biological fluid sample are provided. The filter comprises an outer housing, inlet, and outlet. A plurality of filter surfaces are provided within the outer housing, and at least one coating is applied to the filter surfaces. The at least one...

20060234212 - Hepatitis-b viral variants with reduced susceptibility to nucleoside analogs and uses thereof: The present invention relates generally to the field of Hepatitis B variants exhibiting a reduced sensitivity to nucleoside analogues, both in vivo and in vitro. More in particular, reverse transcriptase mutant rtA181S is provided. Present invention provides assays and methods for detecting such variant, which assays are useful in monitoring...

20060234214 - Methods of detecting hepatitis c virus: The present invention provides a sensitive and specific test for hepatitis C virus. The test detects the presence of particular core antigens of hepatitis C, which appear earlier after initial infection than antigens or viral genetic materials used in previously-developed assays. The invention test allows detection of hepatitis C infection...

20060234209 - Microchip-based system for hiv diagnostics: The invention relates to microchip-based assays to measure HIV-associated analytes of interest (e.g., CD4 lymphocytes, HIV RNA and liver enzymes) in a sample from a subject infected with the HIV virus. Methods of the present invention are optimal for use in monitoring HIV disease in resource-poor settings....

20060234208 - Novel mechanism for hiv-1 entry into host cells and peptides inhibiting this mechanism: This invention relates to the finding that TAT interacts with gp120 at the cell surface and enhances HIV-1 entry into host cells. This invention also discloses modulators of this interaction, particularly peptides that mimic the region of gp120 involved in this interaction. These peptides interfere with TAT-mediated enhancement of infection...

20060234211 - Peptides for detection of antibody to porcine reproductive respiratory syndrome virus: The invention provides compositions and methods for the detection and quantification of PRRSV antibodies and antibody fragments using polypeptides....

20060234215 - Rapid exchange luminescence (rel) for high sensitivity detection: A bioswitch is described which includes a long-lived emitter such as a lanthanide luminophore for time gated detection of ligand binding without interfering background signal....

20060234213 - Synthetic peptides useful in biological essays for detecting infections caused by group o hiv-1 viruses: wherein Δis a biotinyl radical, a biocytinyl radical, a hydrogen atom, an acetyl (CH3CO—) radical, an aliphatic chain which may contain one or two thiol, an aldehyde functional group, or an amine functional group, Z represents peptide sequence -1-Ser-2-, -1-Gln-2-, or -1-Asn-2-, wherein -1 represents a peptide sequence of 0...

20060234223 - Association of snps in the comt locus and neighboring loci with schizophrenia, bipolar disorder, breast cancer and colorectal cancer: Methods and kits used for determining predisposition and/or diagnosis of schizophrenia, bipolar disorder, breast cancer and colorectal cancer using genotypes in the COMT locus are disclosed. Also disclosed are methods and drugs for treating these disorders. Further disclosed are methods and kits useful for prediction drug responsiveness towards mental disorders...

20060234221 - Biallelic markers of d-amino acid oxidase and uses thereof: The invention concerns the human DAO gene, polynucleotides, and biallelic markers. The invention also concerns the association established between schizophrenia and the biallelic markers. The invention provides means to determine the predisposition of individuals to schizophrenia or related CNS disorder, as well as means for the disease diagnosis and prognosis....

20060234267 - Bioarray chip reaction apparatus and its manufacture: A body 300 having a cavity 310 for mounting a substrate 120 fabricated with probe sequences at known locations according to the methods disclosed in U.S. Pat. No. 5,143,854 and PCT WO 92/10092 or others, is provided. The cavity includes inlets 350 and 360 for introducing selected fluids into the...

20060234237 - Biomarkers and methods for determining sensitivity to epidermal growth factor receptor modulators: EGFR biomakers useful in a method for identifying a mammal that will respond therapeutically to a method of treating cancer comprising administering an EGFR modulator, wherein the method comprises (a) exposing the mammal to the EGFR modulator and (b) measuring in the mammal level of at least one biomaker, wherein...

20060234259 - Biomarkers for predicting prostate cancer progression: The present invention relates to the identification and use of gene expression profiles with clinical relevance to prostate cancer. In particular, the invention provides the identity of genes whose expression, at the transcriptional and protein levels, is correlated with prostate cancer progression. Methods and kits are described for using these...

20060234287 - Breast cancer progression signatures: Methods and compositions for the identification of breast cancer progression signatures are provided. The signature profiles are identified based upon multiple sampling of reference breast tissue samples from independent cases of breast cancer and provide a reliable set of molecular criteria for identification of cells as being in one or...

20060234260 - Chlamydia trachomatis genomic sequence and polypeptides, fragments thereof and uses thereof, in particular for the diagnosis, prevention and treatment of infection: The subject of the invention is the genomic sequence and the nucleotide sequences encoding polypeptides of Chlamydia trachomatis, such as cellular envelope polypeptides, which are secreted or specific, or which are involved in metabolism, in the replication process or in virulence, polypeptides encoded by such sequences, as well as vectors...

20060234254 - Colon cancer biomarker discovery: The present application discloses an epigenetic marker for colon cancer....

20060234261 - Colorimetric readout of hybridization chain reaction: The present invention relates to the use of calorimetric hybridization chain reaction (HCR) to detect the presence of one or more target analytes in a sample. In the preferred embodiments, metastable nucleic acid monomers are provided that associate in the presence of an initiator nucleic acid. Upon exposure to the...

20060234248 - Composite organic inorganic nanoclusters: Metallic nanoclusters capable of providing an enhanced Raman signal from an organic Raman-active molecule incorporated therein are provided. The nanoclusters may be further functionalized, for example, with coatings and layers, such as adsorption layers, metal coatings, silica coatings, probes, and organic layers. The nanoclusters are generally referred to as COINs...

20060234247 - Correction of alpha-1-antitrypsin genetic defects using spliceosome mediated rna trans splicing: The present invention provides methods and compositions for generating novel nucleic acid molecules through targeted spliceosomal mediated RNA trans-splicing. The compositions of the invention include pre-trans-splicing molecules (PTMs) designed to interact with a SERPINA1 target precursor messenger RNA molecule (target pre-mRNA) and mediate a trans-splicing reaction resulting in the generation...

20060234240 - Diagnostics and therapeutics for diseases associated with 5-hydroxytryptamine 1f receptor (5-ht1f): The invention provides a human 5-HT1F which is associated with the hematological diseases, disorders of the peripheral and central nervous system, gastrointestinal diseases, cardiovascular diseases, cancer, muscle/skeleton disorders, respiratory diseases and urological diseases. The invention also provides assays for the identification of compounds useful in the treatment or prevention of...

20060234256 - Double stranded rna receptor (dsrna-r) and methods relating thereto: The present invention is directed to nucleic acid molecules and polypeptides encoding a dsRNA receptor (dsRNA-R). The dsRNA-R contains a THD, interacts with the MyD88 adapter protein, and may bind to dsRNA. The present invention is also directed to antibodies against dsRNA-R and to methods of modulating an immune response...

20060234280 - Enyzmes having alpha amylase activity and methods of making and using them: The invention relates to alpha amylases and to polynucleotides encoding the alpha amylases, and methods of making and using them. In addition methods of designing new alpha amylases and methods of use thereof are also provided. The alpha amylases have increased activity and stability at increased pH and temperature....

20060234283 - Fungal cell wall synthesis gene: A reporter system reflecting the transport process that transports GPI-anchored proteins to the cell wall was constructed and compounds inhibiting this process were discovered. Further, genes conferring resistance to the above compounds were identified and methods of screening for compounds that inhibit the activity of the proteins encoded by these...

20060234246 - Gene products differentially expressed in cancerous cells: The present invention provides polynucleotides, as well as polypeptides encoded thereby, that are differentially expressed in cancer cells. These polynucleotides are useful in a variety of diagnostic and therapeutic methods. The present invention further provides methods of reducing growth of cancer cells. These methods are useful for treating cancer....

20060234216 - Haplotype structures of chromosome 21: The present invention includes the use of any of the polymorphisms, SNP haplotype blocks or SNP haplotype patterns. In one embodiment, susceptibility to a phenotype resulting from an allele or marker in linkage disequilibrium with such polymorphic forms is evaluated. Novel therapeutic and diagnostic compounds and methods are also disclosed....

20060234225 - Height-related gene: The application relates to isolated regions and genes from the Y chromosome which encompass the Y specific growth gene GCY. Probes and primers are also provided....

20060234227 - Helix-hairpin-helix motifs to manipulate properties of dna processing enzymes: The utility of Topo V's HhH motifs for modulating DNA binding properties of a DNA processing enzyme, such as Taq DNA polymerase or PfuDNA polymerase, is demonstrated. In one embodiment, an amino acid residue comprising one or more HhH domains derived from Topoisomerase V, linked to either the NH2-terminus or...

20060234286 - Human paris-1 antigen and nucleic acids: diagnostic and therapeutic uses: The present invention relates to the discovery, identification and characterization of a novel tumor suppressor gene designated PARIS-1. The invention includes nucleotide sequences of the PARIS-1 gene and amino acid sequences of its encoded protein product(s), as well as fragments, derivatives and analogs thereof. The invention also includes the production...

20060234273 - Id-1 and id-2 genes and products as markers of epithelial cancer: A method for detection and prognosis of breast cancer and other types of cancer. The method comprises detecting expression, if any, for both an Id-1 and an Id-2 genes, or the ratio thereof, of gene products in samples of breast tissue obtained from a patient. When expressed, Id-1 gene is...

20060234269 - Laser modification and functionalization of substrates: Assay devices comprising substrates functionalized to comprise probe species on multiple separate regions are provided. Ten thousand to a hundred thousand separate regions can be provided in a substrate of one square centimeter. The separate regions can comprise separate probe species, or in another embodiment, multiple different probe species can...

20060234268 - Linear amplification of rna: The present application discloses a method for linear amplification of RNA....

20060234236 - Method and device for pcr-amplification and detection of nucleotide sequences: A DNA-Chip includes a flat carrier and an array of spots containing probe molecules (oligonucleotides) which are arranged on said carrier. Each spot is associated with a microelectrode arrangement for impedance spectroscopic detection of binding events occurring between the probe molecules and target molecules (DNA fragments) applied by way of...

20060234224 - Method and nucleic acids for the analysis of colon cell proliferative disorders: The invention provides methods and nucleic acids for detecting, differentiating or distinguishing between colon cell proliferative disorders by analysis of one or more of the genes Versican, TPEF, H-Cadherin, Calcitonin, and EYA4. The invention further provides novel nucleic acid sequences useful for the cell proliferative disorder specific analysis of said...

20060234275 - Method for collecting purified cells: The present invention provides improved methods for collecting cells for subsequent analysis. More specifically, the invention provides methods for collecting purified cell samples from a flow cytometer onto a solid support on which cell lysis occurs and genetic material is immobilized....

20060234253 - Method for detecting a target substance by using a nucleic acid probe: A method for detecting a target substance is described, which comprises the steps of: forming a first complex comprising (a) a first nucleic acid probe immobilized on a first solid phase and having a single-strand portion, (b) a nucleic acid ligand which has a binding section as a base sequence...

20060234219 - Method for detecting and identifying microorganism causative of infection: Causative microorganisms of infectious diseases are detected and/or identified rapidly and high-sensitively by taking phagocytes from the clinical specimens containing active phagocytes, immobilizing the phagocytes so taken, treating the phagocytes to improve cell membrane permeabilities thereof, further treating the phagocytes to bare DNA in the causative microorganisms which might be...

20060234263 - Method for reducing the presence of amplification inhibitors in a reaction receptacle: An automated analyzer for performing multiple diagnostic assays simultaneously includes multiple stations in which discrete aspects of the assay are performed on fluid samples contained in sample vessels. The analyzer includes stations for automatically preparing a sample, incubating the sample, preforming an analyte isolation procedure, ascertaining the presence of a...

20060234277 - Method for selectively blocking hemoglobin rna amplification: This invention provides oligonucleotides, compositions, kits, and methods for specifically blocking amplification of hemoglobin mRNA in a sample of RNA. The oligonucleotides and methods are particularly advantageous for analyzing samples of RNA extracted from whole blood samples....

20060234230 - Method of detecting gene polymorphism: A method for detecting a genetic polymorphism(s) in a gene encoding cytochrome P450 using as oligonucleotide probes and/or oligonucleotide primers at least one sequence selected from the group consisting of an at least 13 nucleotide sequence within any of the nucleotide sequences as shown in SEQ ID NOS: 1 through...

20060234228 - Method of identifying nucleic acid: Test nucleic acids are identified by the steps of: synthesizing multiple nucleic acids that comprise nucleotide sequences complementary to different regions of a test nucleic acid; and comparing the dissociation curve of a mixture of the synthesized nucleic acids. The dissociation curve of a mixture of nucleic acids shows a...

20060234235 - Methods and compositions for the diagnosis of neuroendocrine lung cancer: This invention relates to methods and compositions for the diagnosis of neuroendocrine lung cancers. In particular, the invention concerns the use of cDNA microarrays to facilitate the differential diagnosis of neuroendocrine tumor types....

20060234252 - Methods and kits for methylation detection: Methods for determining the methylation state of at least one target nucleotide that employ a reaction catalyzed by a structure-specific nuclease, typically coupled with a ligation reaction are disclosed. By detecting the cleaved flap, a ligation product, a ligation product surrogate, a hybridization complex, or combinations thereof, one can infer...

20060234266 - Methods for detecting multiple species and subspecies of neisseria: Disclosed are methods, kits, and other components for detecting multiple nucleic acids in a sample. The methods and kits may be useful for detecting Neisseria in a sample. In some embodiments, the methods include (a) reacting a mixture that includes (i) nucleic acid isolated from the sample; (ii) at least...

20060234242 - Methods for identifying therapeutic targets involved in glucose and lipid metabolism: The identification and evaluation of mRNA and protein targets associated with RNA binding proteins or mRNP complexes is described. In particular, the invention provides methods for identifying RNA binding proteins associated with physiological pathways that participate in glucose and lipid metabolism and mRNAs that exhibit coordinated gene regulation across those...

20060234271 - Methods for the diagnosis and treatment of metastatic prostate tumors: The present invention is directed to methods for the identification of a prostate cancer cell that has metastatic potential or a cell that is or is derived from a secondary prostate tumor metastasis by screening for the expression of flt-4, the cellular receptor of vascular endothelial growth factor-C and -D...

20060234274 - Methods of diagnosis: A method to detect whether a female subject is predisposed to POF, the method comprising analysing at least one or more polymorphism in the INHA gene chosen from the group consisting: −124A>G; −16C>T; TG repeat (as herein after described); and one or more polymorphism in the INHA gene which is...

20060234251 - Methods of enhancing isolation of rna from biological samples: A method of enhancing the isolation of ribonucleic acids from samples of biological or cellular material is disclosed which uses a solution of an Agent during binding of nucleic acids onto a solid phase binding material which, after elution, enhances the recovery of RNA. Washing the solid phase and eluting...

20060234250 - Methods of screening and compositions for life span modulators: Identification of nucleic acids involved in life span diseases and disorders or related diseases and disorders, and the use of such methods for identifying candidate agents which modulate life span diseases and disorders or related diseases and disorders are provided. Compositions and methods for treating life span diseases and disorders...

20060234276 - Methods of treatment of type 2 diabetes: The present invention relates generally to the field of diabetes. More particularly, it concerns the identification of genes responsible for NIDDM1 for use in diagnostic and therapeutic applications. The present invention demonstrates that the NIDDM1 locus is, in fact, the calpain 10 gene. The invention further relates to the discovery...

20060234284 - Methods of using molecular constructs for detection of biochemical reactions: The present invention relates to molecular constructs and methods of their use in detecting biochemical reactions. In particular, the invention relates to a molecular construct having a capture portion and a substrate portion, where the capture portion isolates the construct from a sample medium, and the substrate portion enables the...

20060234231 - Microarrays displaying encoded molecules: Disclosed is a microarray comprising a plurality of single stranded nucleic acid probes immobilized in discrete areas of a solid support, said probes being hybridised to a library of complexes, wherein each complex comprises an encoded molecule and a template which codes for said molecule, said template comprising a number...

20060234265 - Microarrays having multi-functional, compartmentalized analysis areas and methods of use: Microarrays are provided that include multiple analysis areas. Each analysis area can include first and second active areas, and first and second hydrophobic areas. The first hydrophobic area surrounds the first active area, and the second active area surrounds the first hydrophobic area. The microarrays are useful in analytical chemistry,...

20060234241 - Microchip, nucleic acid extracting kit, and nucleic acid extracting method: A new technical means is provided that can be produced and prepared at low cost, and in which the generation of stagnation in the flow of a liquid sample can be suppressed, microbeads can be smoothly and simply injected, filled and transported and the generation of dust can be also...

20060234239 - Modulation of insulin like growth factor i receptor expression: The present invention provides compositions and methods for modulating the expression of growth factor gene. In particular, this invention relates to compounds, particularly oligonucleotide compounds, which, in preferred embodiments, hybridize with nucleic acid molecules encoding the Insulin Like Growth Factor I receptor (IGF-I receptor or IGF-IR) and in particular human...

20060234264 - Multiplex polynucleotide synthesis: The invention provides a method of synthesizing complex mixtures of long polynucleotides by separately synthesizing and assembling shorter component oligonucleotides. In one aspect, pairs of oligonucleotides that form components of such polynucleotides are synthesized on one or more microarrays, or other large-scale parallel solid phase synthesis platforms, after which they...

20060234226 - Non-affinity purification of proteins: The present invention relates to a method for protein purification that involves the combination of non-affinity chromatography with HPTFF....

20060234270 - Normalization methods for gene expression analysis: The invention provides methods for obtaining an expected concentration for each of a plurality of housekeeping genes and using these values to obtain normalized concentrations for genes in an experimental sample using both external transcripts and internal housekeeping genes. A standard calibration curve is prepared using intensity values and concentrations...

20060234249 - Novel assays for assessing cancerous cell growth: The present invention provides novel assays for assessing cancerous cell growth. The invention is useful for the identification and validation of oncogenes and tumor suppressors, as well as for the identification and validation of therapeutic compounds for the treatment of cancer....

20060234281 - Novel galanin receptor: The present invention is directed to a novel receptor for galanin which has been designated as galanin receptor 2. The invention encompasses both the receptor protein as well as nucleic acids encoding the protein. In addition, the present invention is directed to methods and compositions which rely upon either GAL-R2...

20060234282 - Novel galanin receptor: The present invention is directed to a novel receptor for galanin which has been designated as galanin receptor 2. The invention encompasses both the receptor protein as well as nucleic acids encoding the protein. In addition, the present invention is directed to methods and compositions which rely upon either GAL-R2...

20060234290 - Novel genes and expression products therefrom: The present invention relates generally to the identification of the products of gene expression in cancerous tissue or other tissue associated with an aberrant medical condition. Identification of such expression products enables the development of a range of diagnostic and therapeutic agents. In one embodiment of the present invention, a...

20060234229 - Novel method for monitoring biomolecular interactions: The present invention relates to methods for monitoring interactions between target-molecules and at least one analyte comprising: (a) contacting a plurality of target-molecules, said target-molecules being immobilized on a porous substrate, with a sample, said sample comprising at least one analyte; (b) incubating said target-molecules with said sample under conditions...

20060234232 - Novel n-acetylgalactosamine transferases and nucleic acids encoding the same: An enzyme which transfers N-acetylgalactosamine to N-acetylglucosamine via a β1-4 linkage was isolated and the structure of its gene was explained. This led to the production of said enzyme or the like by genetic engineering techniques, the production of oligosaccharides using said enzyme, and the diagnosis of diseases on the...

20060234220 - Novel n-acetylglucosamine transferase, nucleic acid encoding the same, antibody against the same and use thereof for diagnosing cancer or tumor: Described are an enzyme having an activity to transfer N-acetylglucosamine to N-acetylgalactosaminyl group through a β1,3-linkage; nucleic acid coding for the enzyme; and method for diagnosis of a cancer and/or tumor, especially a cancer and/or tumor of a digestive organ using the expression amount of the gene of the enzyme...

20060234255 - Novel proteins and nucleic acids encoding same: The present invention provides novel isolated polynucleotides and small molecule target proteins encoded by the polynucleotides. Antibodies that immunospecifically bind to a novel small molecule target protein or any derivative, variant, mutant or fragment of that protein, polynucleotide or antibody are disclosed, as are methods in which the small molecule...

20060234234 - Nucleic acid amplification primers for pcr-based clonality studies: The invention relates to PCR-based clonality studies for among others early diagnosis of lymphoproliferative disorders. Provided is a set of nucleic acid amplification primers comprising a forward primer, or a variant thereof, and a reverse primer, or a variant thereof, capable of amplifying a rearrangement selected from the group consisting...

20060234288 - Nucleic acid encoding proteins involved in protein degradation, products and methods related thereto: In accordance with the present invention, there are provided novel Siah-Mediated-Degradation-Proteins (SMDPs) and/or SCF-Complex Proteins (SCPs). Nucleic acid sequences encoding such proteins and assays employing same are also disclosed. The invention SMDPs and/or SCPs can be employed in a variety of ways, for example, for the production of anti-SMDP and/or...

20060234218 - P450 proteins: This invention relates to proteins, termed BAA92678.1 and BAA31683.1, herein identified as P450 enzymes and to the use of these proteins and nucleic acid sequences from the encoding genes in the diagnosis, prevention and treatment of disease....

20060234245 - Pathogenic gene for coronary artery disease: A method of determining a person at risk of developing coronary artery disease includes detecting an alteration of at least one of an MEF2A gene, genes regulated by MEF2A transcription factor, or genes that regulate expression of MEF2A transcription factor of the patient. The alteration substantially reduces the transcription activity...

20060234262 - Physiogenomic method for predicting clinical outcomes of treatments in patients: A physiogenomic-based method for predicting the outcome of treatment regimens in human patients based upon association screening to identify genetic markers and related physiological characteristics that influence the disease status of a patient, the progression to disease and response to the treatment. By repeating the analysis quantitatively for each of...

20060234238 - Polymerase-based protocols for generating chimeric oligonucleotides: The present invention relates to methods of generating chimeric oligonucleotides without the need for subcloning. The methods of the invention are polymerase-based, and may optionally be adapted for use with reagents available in commercially available mutagenesis kits. Major applications of this method include directed evolution and other areas that benefit...

20060234233 - Random transposon insertion in staphylococcus aureus and use thereof to identify essential genes: The invention provides a method for generating a database of candidate essential genes in Staphylococcus aureus, as well as otherwise important genes that, when mutated, lead to a growth attenuated phenotype. Such genes and mutants of such genes are important for identifying antibacterial agents suitable for treating and preventing S....

20060234243 - Sample preparing unit: The invention relates to a unit for preparing reaction mixtures for chemical reactions, in particular polymerase chain reactions. The inventive unit comprises an input, output and at least one membrane. Said invention is characterised in that a compound bound to the membrane contains substances necessary for carrying out chemical reactions....

20060234279 - Screening using polarization anisotropy in fret emissions: Methods and apparatus are described for detecting specific binding between first and second chemical entities. The first chemical entity in association with a first fluorophore is immobilized. The second chemical entity is allowed to bind with the immobilized first chemical entity. The second chemical entity is or becomes coupled to...

20060234278 - Sequences used to identify if a female mammal comprises a mutation in the alpha-fetoprotein sequence(s) or a partial or total deletion of the alpha-fetoprotein sequence(s): The present invention is related to specific sequences, preferably present in a diagnostic kit to identify if a female mammal comprises in her genome a mutation in the alpha-fetoprotein sequence or a partial or total deletion of this alpha-fetoprotein sequence, present heterozygously or homozygously (on both allele)....

20060234217 - Shrimp alkaline phosphatase: e

20060234222 - Soluble recombinant protein production: Described is a method of producing a soluble bioactive domain of a protein, the method comprising the step of selecting suitable soluble subunits of a protein and assessing the produced protein for desired activity. The method may comprise the steps of amplifying DNA encoding at least one candidate soluble domain,...

20060234289 - Sp110, a polypeptide component of the nuclear body: Cloning and characterization of a full length cDNA encoding Sp110 (speckled 110), a novel 110 kDa polypeptide, is disclosed. It is disclosed that Sp110 is a component of the nuclear body, is expressed in leukocytes, and is also expressed in other types of cells, including endothelial cells, smooth muscle cells,...

20060234258 - Stanniocalcin (stc1) mrna assay method: The present invention provides an RNA amplification process, comprising steps in which double-stranded DNA containing a promoter sequence is produced using a first primer and second primer, at least one of which has the promoter sequence at the 5′ end, and reverse transcriptase, the double-stranded DNA is used as template...

20060234244 - System for analyzing bio chips using gene ontology and a method thereof: Disclosed is a system for analyzing a bio chip using Gene Ontology(hereinafter referred to “GO”) and a method thereof. According to a preferred embodiment of the present invention, it is provided a system for analyzing a bio chip comprising: a GO(gene ontology) term assigning part for receiving a statistical clustering...

20060234257 - Therapeutic polypeptides, nucleic acids encoding same, and methods of use: Disclosed herein are nucleic acid sequences that encode novel polypeptides. Also disclosed are polypeptides encoded by these nucleic acid sequences, and antibodies that immunospecifically bind to the polypeptide, as well as derivatives, variants, mutants, or fragments of the novel polypeptide, polynucleotide, or antibody specific to the polypeptide. The invention further...

20060234285 - Tumor necrosis factor receptors 6 alpha & 6 beta: The present invention relates to novel Tumor Necrosis Factor Receptor proteins. In particular, isolated nucleic acid molecules are provided encoding the human TNFR-6α & -6β proteins. TNFR-6α & -6β polypeptides are also provided as are vectors, host cells and recombinant methods for producing the same. The invention further relates to...

20060234272 - Using gene panels to predict tissue sensitivity to ionizing radiation: A method of predicting sensitivity to radiation comprises selecting at least one of a first panel of genes associated with increased chromosomal damage and a second panel of genes associated with reduced chromosomal damage. The tissue is exposed to radiation. The RNA of the tissue is measured providing measured RNA...

20060234306 - Assay method: A new method for assaying the ability of a compound to block the binding of an α4 integrin to a binding partner thereof provides a useful screening tool....

20060234300 - Cardiac hormones for assessing cardiovascular risk: The present invention relates to the use of cardiac hormones, particularly natriuretic peptides, for diagnosing the cardiovascular risk of a patient who is a candidate for adminitstration of a cox-2-inhibiting compound, in particular an NSAID, selective cox-2 inhibitior, or steroid. More particularly, the present invention relates to the use of...

20060234304 - Diagnosis of acute myocardial, ischemic diseases: A method for the diagnosis of acute myocardial, ischaemic diseases, for example acute myocardial infarct, particularly without increasing ST-paths in EKG (NSTEMI). At least two markers on a patient, who is to examined, are determined. A kit for carrying out said diagnostic method, including a test strip for carrying out...

20060234294 - Functional domain and associated molecule of dock2 essentially required in lymphocyte migration: The present invention is related to provide a method for screening a substance interfering in the association of DOCK2 and ELMO1, a method for screening a substance interfering in the association of ELMO1 and Tiam1, and a method for searching a therapeutic agent for immune related diseases such as allergy,...

20060234305 - Gene encoding g-protein coupled receptor and gene product thereof: The present invention, the objects of which are to find out a gene encoding a protein having functions equivalent to those of G-protein-coupled receptor (GPCR) and the protein, and to provide a method for identifying a compound that regulates functions and/or expression of the protein, and to provide a useful...

20060234302 - Libraries and methods for isolating antibodies: The disclosure features methods for identifying antibodies that bind to a carbohydrate moiety. Libraries coding for antibodies that bind to a carbohydrate moiety are provided. The libraries can be provided by modifying a pre-existing nucleic acid library. Antibodies that bind to a carbohydrate moiety are described....

20060234308 - Method for determining the substrate specificity of an enzymatic activity and a device therefor: The invention relates to a method for determining the substrate specificity of an enzymatic activity comprising the following steps: providing an assembly comprising a plurality of amino acid sequences on a planar surface of a support material, whereby the amino acids are directionally immobilised; contacting and/or incubating of an enzymatic...

20060234301 - Method for diagnosing multiple sclerosis: Disclosed is a method for diagnosing multiple sclerosis and more particularly to a method for diagnosing multiple sclerosis by measuring levels of antibodies to glycans in a biological sample....

20060234296 - Method for generating high affinity antibodies: The invention relates to high affinity human monoclonal antibodies, particularly those directed against isotypic determinants of immunoglobulin E (IgE), as well as direct equivalents and derivatives of these antibodies. These antibodies bind to their respective target with an affinity at least 100 fold greater than the original parent antibody. These...

20060234303 - Methods for the design of libraries of protein variants: Methods for designing libraries of protein variants are provided....

20060234292 - Methods of diagnosing and treating diabetes and insulin resistance: The present invention provides compositions and methods for diagnosing and treating diabetes and insulin resistance. In particular, the invention provides methods of identifying modulators of the polynucleotides or polypeptides of the invention and using those modulators to treat diabetes, as well as methods of diagnosing diabetes by measuring the levels...

20060234307 - Modified peptides as therapeutic agents: The present invention concerns fusion of Fc domains with Ang-2 binding peptides and a process for preparing such molecules. In this invention, pharmacologically active compounds are prepared by a process comprising (a) selecting at least one random peptide that binds to Ang-2; and (b) preparing a pharmacologic agent comprising an...

20060234293 - Polypeptide methods and means: The structure of a RAD51-BRC repeat sequence complex structure is provided. The structure can be used in modelling the interaction of molecular structures such as potential pharmaceutical compounds. Mutant RAD51 and BRCA2 polypeptides and RAD51-BRC repeat sequence chimaera proteins and are also provided. The mutants may be used in assays...

20060234299 - Protein scaffolds and uses thereof: Specific monomer domains and multimers comprising the monomer domains are provided. Methods, compositions, libraries and cells that express one or more library member, along with kits and integrated systems, are also included in the present invention....

20060234295 - Sandwich immunoassay for identifying partial proanp peptides: Disclosed is an improved sandwich immunoassay for identifying partial proANP peptides in cardiac and sepsis diagnosis by using two antibodies which specifically bond to partial sequences in the mid-regional area of NT-proANP, extending from amino acid 53 to amino acid 83 of NT-proANP....

20060234297 - Screening method: The present invention provides a screening means for a novel therapeutic drug for arteriosclerosis capable of suppressing the progression of arteriosclerosis due to infection with Chlamydia pneumoniae. Specifically, provided is a screening method for a therapeutic substance for arteriosclerosis and/or a prophylactic/therapeutic substance for complications thereof, which comprises using cells...

20060234291 - Site selective acylation: Disclosed are novel polypeptides of a four helix bundle formed of two dimerized helix-loop-helix motifs, wherein either both have a sequence according to SEQ. ID No. 1, SEQ. ID No. 2, SEQ. ID No. 3, SEQ. ID No. 4, SEQ. ID No. 5, or SEQ. ID No. 7, or one...

20060234298 - Systems and methods for rapidly changing the solution environment around sensors: The invention provides microfluidic systems for altering the solution environment around a nanoscopic or microscopic object, such as a sensor, and methods for using the same. The invention also provides a system and methods for modulating, controlling, preparing, and studying receptors....

20060234312 - Detection of surface-associated human leukocyte elastase: In order to accurately and reliably quantitate HLE on the plasma membranes of the lymphocytes and mononuclear phagocytes, a test sample containing the lymphocytes and mononuclear phagocytes is initially treated with a first antiserum specific for CD4 receptors on the plasma membrane or with a second antiserum specific for chemokine...

20060234310 - Enriched antigen-specific t-cells and related therapeutic and prophylactic compositions and methods: T-cell responses are initiated via contact with MHC/peptide complexes on antigen presenting cells (APCs). The fate of these complexes, however, is unknown. Here, using live APCs expressing MHC class I molecules fused with green-fluorescent protein, we show that peptide-specific T-cell/APC interaction induces clusters of MHC I molecules to congregate within...

20060234311 - Isolating cells expressing secreted proteins: A method of detecting and isolating cells that produce any secreted protein of interest (POI), comprising: a) constructing a cell line transiently or stably expressing a cell surface capture molecule, which binds the POI, by transfecting the cell line with a nucleic acid that encodes such cell surface capture molecule;...

20060234313 - Modulation of angiogenesis through targeting of arginyl transferase (ate1): The invention provides methods and compositions for modulating angiogenesis in a subject. The methods of modulating angiogenesis in a subject include administering to the subject a modulator of N-terminal arginylation activity. The invention also provides a method of identifying such a modulator and a method of in vitro screening for...

20060234309 - Quality assays for antigen presenting cells: The present invention provides methods for evaluating the quality of a preparation of antigen presenting cells, such as dendritic cells. Assays for antigen-independent co-stimulation of T cells and for presentation of predetermined antigen by APC's are provided....

20060234315 - Markers for sudden death in heart failure: A method for screening, diagnosis or prognosis of sudden death in heart failure in a mammalian subject, for determining sudden death in heart failure in a mammalian subject, for identifying a mammalian subject at risk of sudden death in heart failure, or for monitoring the effect of therapy administered to...

20060234316 - Methods for distinguishing type-1 from type-2 diabetes: The present invention provides a new method for the prediction of, or diagnosis of, auto-immune diseases, thereby alerting the subject to the presence of, or propensity to develop, an auto-immune disease so that preventative or therapeutic regiments may be initiated or changed so as to treat, modulate or prevent expansion...

20060234317 - Method of screening: A method of detecting the presence of a functionally inhibitory immunointeractive molecule in a biological sample. Preferably, the immunointeractive molecule is directed to a pathogen derived antigen and, more particularly, a parasite derived antigen and, even more particularly, a Plasmodium drived antigen. The method of the present invention facilitates detection...

20060234318 - Cancer associated gene mina 53, protein mina 53 and monoclonal antibody thereof: Myc protein is an unevenly distributed intermediate agent for cell proliferation, and activates a gene expression via E.box. Mina 53 gene encodes a protein of 53 kDa molecular weight and is present in the nucleoplasm and nucleolus. Mina 53 mRNA and protein expression are induced by artificial introduction of c-Myc...

20060234319 - Disease determination method, data generation method for disease determination and data generation system for disease determination: Supernatant of the urinary sample (SP1) was mixed with an acid or alkali and heated (SP2) and the sample for fluorescence assay was prepared after adjusting its acidity to alkaline (SP3). Next, using fluorometry, three-dimensional fluorescence spectrum consisted of an excitation light wavelength, fluorescence wavelength and fluorescence intensity (SP4). Then,...

20060234320 - Pyroglutamyl peptidase and its gene: The present invention provides DNA encoding novel pyroglutamyl peptidase derived from Aspergillus oryzae, pyroglutamyl peptidase which is produced by using the DNA, and a method for producing a protein lysate with a good flavor at a high hydrolysis rate....

20060234322 - Ehrlichia canis diva (differentiate infected from vaccinated animals): Ehrlichia canis antigens that can be used to differentiate E. canis infected animals from animals that have been challenged with E. canis, e.g., vaccinated against E. canis, are disclosed. The invention also provides compositions and methods for determining the presence of E. canis antigens and antibodies....

20060234321 - Syphilis diagnostic tests and kits: The present invention provides methods and kits for determining whether a human subject is infected with T. pallidum, and isolated fragments of T. pallidum protein Tp92 that can be used in the methods and kits of the invention....

20060234314 - Selective binding and analysis of macromolecules: There is disclosed a method for selectively binding micromolecules having a lysine functionality comprising the steps of: providing a sample containing one or more species of macromolecules, each having a lysine functionality; providing a binding reagent having the formula X—NH—C(═NH)—OR or X—L—NH—C(═NH)—OR where X is an affinity label that selectively...

20060234323 - Luminescent atp detection with extended linearity: A luminescent assay capable of detecting a wider range of ATP concentrations is provided....

20060234324 - Method for enhancing activity of luciferase with fluorescence activity: Methods for enhancing luminescence of a luciferase (BFP-aq) with fluorescence activity derived from a calcium-binding photoprotein are provided. To a luciferase solution with fluorescence activity that contains an apoprotein, a calcium-binding photoprotein, which is constituted such that a coelenteramide or an analog thereof is coordinated inside, a coelenterazine that is...

20060234325 - Hematological assay and kit: The present invention is directed to a kit and a method for a fast and direct determination of the coagulation potential of a sample of blood or plasma utilising a thrombin substrate. The kit comprises at least one activator of the plasmatic substrate with a KM preferably coagulation system and...

20060234328 - Anthrax lethal factor is a mapk kinase protease: The present invention relates to in vitro and ex vivo methods of screening for modulators, homologues, and mimetics of lethal factor mitogen activated protein kinase kinase (MAPKK) protease activity, as well as methods of treating cancer by administering LF to transformed cells....

20060234326 - Mass spectrometry methods for simultaneous detection of metabolic enzyme activity and metabolite levels: The present invention provides a method for detecting a metabolic disorder in an individual. The method involves (a) contacting a sample containing (i) one or more metabolically indicative enzymes and (ii) one or more metabolic analytes, with one or more substrates for said one or more enzymes to produce a...

20060234327 - Methods for identifying enzyme inhibitors and protein kinases: The present invention provides methods for identifying compounds that inhibit kinases. In addition, methods for profiling protein kinases are also provided. In addition, methods for determining the mode of action of kinase inhibitors are also provided....

20060234329 - Method for determining the antiradical defense potential and use thereof, in particular in veterinary and human preventive therapeutics: The present invention relates to a method for determining in vitro the global antiradical defense potential of a living organism or a physical, chemical or biological agent. Said method, which comprises the use of free radicals as a means for inducing cellular lysis and the evaluation of said cellular lysis,...

20060234330 - Variable resistance sterilization process challenge device and method: A sterilization process challenge device has a sterilization indicator contained within a container; and a variable diffusion restriction into said container....

20060234331 - Method of selecting escherichia coli strain overexpressing foreign gene escherichia coli mutant thus selected and process for producing enzyme and compound using the same: s

20060234332 - Predicting hepatotoxicity using cell based assays: Cell based assays are used to assess the hepatotoxicity of a stimulus. Imaging technologies are used to analyze the effects of a stimulus on hepatocytes. Image analysis may characterize the stimulus on the basis of whether it is hepatotoxic, and if so what type of pathology is exhibited; e.g., apoptosis,...

20060234334 - Bacterial hemoglobin genes and their use to increase carotenoid production: Genes encoding bacterial oxygen binding proteins are provided. Recombinant expression of at least one of the present bacterial hemoglobin genes increased the growth characteristics and/or carotenoid production levels in microbial host cells grown under microaerobic conditions....

20060234333 - Method for producing carotenoids or their precursors using genetically modified organisms of the blakeslea genus, carotenoids or their precursors produced by said method and use thereof: The invention relates to a method for producing carotenoids or their precursors using genetically modified organisms of the Blakeslea genus. Said method comprises the following steps (i) transformation of at least one of the cells, (ii) optional homokaryotic conversion of the cells obtained in step (i) to produce cells, in...

20060234335 - Conjugated thiophene compound, conprising perfluorinated and alkylated sidechains, conductive organic thin film containing the compound, and field-effect type organic transistor employing the thin film: where R1 and R2 are hydrogen or a substituted or unsubstituted linear, cyclic, or branched alkyl group of 1 to 20 carbon atoms, at least one of R1 and R2 being not hydrogen; and R3 and R4 are hydrogen or a substituted or unsubstituted linear, cyclic, or branched perfluoroalkyl group...

20060234350 - Endogenous and non-endogenous versions of human g protein-coupled receptors: The invention disclosed in this patent document relates to transmembrane receptors, more particularly to a human G protein-coupled receptor for which the endogenous ligand is unknown, and to mutated (non-endogenous) versions of the human GPCRs for evidence of constitutive activity....

20060234346 - Expression of mammalian proteins in pseudomonas fluorescens: The invention is a process for improved production of a recombinant mammalian protein by expression in a Pseudomonad, particularly in a Pseudomonas fluorescens organism. The process improves production of mammalian proteins, particularly human or human-derived proteins, over known expression systems such as E. coli in comparable circumstances Processes for improved...

20060234345 - Expression of soluble, active eukaryotic glycosyltransferases in prokaryotic organisms: The present invention provides enhanced methods of producing soluble, active eukaryotic glycosyltransferases in prokaryotic microorganisms that have an oxidizing environment....

20060234337 - Expression system for actinomycete-origin cytochrome p-450 in escherichia coli: This invention relates to a system for the expression of cytochrome P-450 gene in host Escherichia coli, and provides Escherichia coli which contains actinomycete ferredoxin gene and also ferredoxin gene and ferredoxin reductase gene which are xenogenic to Escherichia coli. Thus, this invention is useful for the promotion of effective...

20060234349 - Fungal cell wall synthesis gene: A reporter system reflecting the transport process that transports GPI-anchored proteins to the cell wall was constructed and compounds inhibiting this process were discovered. Further, genes conferring resistance to the above compounds were identified and methods of screening for compounds that inhibit the activity of the proteins encoded by these...

20060234342 - Human dna topoisomerase i alpha: Disclosed is a human is a hTopI-α polypeptide and DNA (RNA) encoding such hTopI-α polypeptide. Also provided is a procedure for producing such polypeptide by recombinant techniques and antibodies and antagonists against such polypeptide. Also provided are methods of using the antibodies and antagonist inhibitors to inhibit the action of...

20060234340 - Human heavy chain antibody expression in flamentous fungi: The present invention relates to a method for producing a functional human immunoglobulin, wherein a human heavy chain immunoglobulin, devoid of any light chain, is expressed, comprising the steps of: a) transforming a filamentous host cell with a recombinant construct encoding a modified human heavy chain immunoglobulin, wherein the modifications...

20060234338 - Isomaltose synthase-knockout microorganism belonging eumycota: It is intended to provide a host microorganism whereby the expression of a gene encoding a target protein can be effectively elevated in the case of transferring the gene. It is also intended to provide a transformant whereby a target protein can be produced at a high efficiency. It is...

20060234339 - Method of expressing protein having unnatural amino acid integrated thereinto: A method of expressing a protein having an unnatural amino acid integrated thereinto characterized by comprising expressing (A) a mutant tyrosyl tRNA synthase which is a mutant of Escherichia coli-origin tyrosyl tRNA synthase and has an elevated specificity for a unnatural tyrosine derivative compared with the specificity for tyrosine, (B)...

20060234348 - Method of production of rna polymerase protein: A method of forming a stable RNA polymerase enzyme by expressing protein 2a in cells, pelleting the cells expressing protein 2a, macerating the cells expressing protein 2a to obtain a cell lysate, and filtering the cell lysate through an affinity resin. A stable RNA polymerase enzyme for copying RNA in...

20060234336 - Methylotrophic bacterium for the production of recombinant proteins and other products: The present invention relates to a method of producing a recombinant peptide, a recombinant protein, or a product from metabolic engineering using a genetically modified methylotrophic bacterium, and more particularly to Methylobacterium extorquens ATCC 55366. The method comprises introducing am expression vector into the methylotrophic bacterium, the expression vector comprising...

20060234341 - Process for producing phosphorylase: It is intended to provide a process for conveniently producing phosphorylase at a high purity using a phosphorylase-producing microorganism. Namely, a process for producing phosphorylase characterized by culturing a phosphorylase-producing microorganism in a medium containing phosphoric acid or its salt at a concentration of 50 mM or above and collecting...

20060234344 - Protein kinases: The present invention relates to novel kinase polypetides, nucleotide sequences encoding the novel kinase polypetides, as well as various products and methods useful for the diagnosis and treatment of various kinase-related disease and conditions....

20060234347 - Targeting multiple angiogenic pathways for cancer therapy using soluble tyrosine kinase receptors: Multivalent soluble receptor proteins that bind to more than one angiogenic factor are described. Nucleotide and vector sequences which encode the multivalent soluble receptor protein, as well as host cells which comprise them and methods of making and using them are also described. The multivalent soluble receptor proteins and vectors...

20060234343 - Truncated egf receptor: The present invention relates to truncated EGF receptor molecules that exhibit increased binding affinities for EGFR ligands such as EGF and TGF1. The present invention also relates to methods of screening for EGF receptor ligands and methods of treatment which involve the use of these molecules....

20060234352 - Homogeneity and secretion of recombinant proteins in mammalian systems: The present invention relates to a method for improving homogeneity and/or secretion of a recombinant protein of interest expressed in mammalian cells by replacing the endogenous signal peptide sequence of the DNA encoding the protein of interest with that of human hGH. Specifically, the present invention relates to a method...

20060234351 - Yeast protein expression secretion system: This invention discloses novel prepro-insulin polypeptides. The polypeptides consist of an N-terminal region, derived from N-terminal regions of secretory proteins, and a downstream insulin polypeptide region. The N-terminal region directs the polypeptides efficiently into the secretory pathway of yeasts. Modifications at the N-terminal region, just adjacent to the insulin polypeptide...

20060234353 - Strain belonging to the genus streptomyces and being capable of producing nemadictin and process for producing nemadictin using the strain: In the present invention, a recombination of gene groups of nemadectin aglycon biosynthesis is performed for obtaining C-13 hydroxylnemadectin, to which sugar groups can be attached, and a production strain which produces C-13 hydroxylnemadectin is produced. Further, C-13 glycosylnemadectin producing strain is prepared by introducing aveBI-BVIII genes involving glycosidation of...

20060234354 - Preparation of fucosylated oligosaccharides: This invention provides methods for practical enzymatic conversion of GDP-mannose to GDP-fucose. These methods are useful for efficient synthesis of reactants used in the synthesis of fucosylated oligosaccharides....

20060234355 - Alpha-agarase and process for producing the same: A polypeptide having an α-agarase activity, a gene encoding the polypeptide, a method for producing the polypeptide by genetic engineering and a method for producing an agarooligosaccharide using the polypeptide....

20060234356 - Novel polynucleotides encoding useful polypeptides in corynebacterium glutamicum ssp. lactofermentum: The present invention provides novel polypeptides and polynucleotides useful as biotechnological tools, specifically identified in a coryneform bacterium Corynebacterium glutamicum ssp. lactofermentum and methods of producing substances in organisms having enhanced or attenuated expression of these polypeptides and/or polynucleotides....

20060234357 - Novel polynucleotides encoding useful polypeptides in corynebacterium glutamicum ssp. lactofermentum: The present invention provides novel polypeptides and polynucleotides useful as biotechnological tools, specifically identified in a coryneform bacterium Corynebacterium glutamicum ssp. lactofermentum and methods of producing substances in organisms having enhanced or attenuated expression of these polypeptides and/or polynucleotides....

20060234358 - Method for the microbial production of aromatic amino acids and other metabolites of the aromatic amino acid biosynthetic pathway: A process for the microbial production of aromatic amino acids and other metabolites of the aromatic amino acid biosynthetic pathway The invention relates to a process for the microbial production of aromatic amino acids and other metabolites of the aromatic amino acid biosynthetic pathway. Microbially produced substances such as fine...

20060234359 - Method for the production or r-a-lipoic acid by fermentation: The invention relates to a method for the production of R-α-lipoic acid by fermentation, characterised in that a cell with a weakened lipoyl protein ligase A activity is cultivated in a culture medium, whereby the cell precipitates enantiomerically pure R-α-lipoic acid in the free form in the culture medium and...

20060234360 - Ascorbic acid production from d-glucose in yeast: Herein is disclosed a method of generating ascorbic acid from yeast transformed with a mannose epimerase. In a further embodiment, the yeast can be further transformed with a myoinositol phosphatase. In the method, the transformed yeast can produce L-ascorbic acid from D-glucose. The transformed yeast has been observed to have...

20060234361 - Method for producing l-lactic acid: A method for producing L-lactic acid is disclosed, which comprises the following steps: (a) culturing spores of Rhizopus oryzae in a medium containing a carbon source and a nitrogen source, forming dispersed mycelia, (b) seeding said mycelia into a fermentation medium containing ammonia; (c) starting the fermentation, meanwhile, the pH...

20060234362 - Enzymatic method of making 1,2-diol derivatives and their esters with succine anhydride: The present invention relates to a new process for the preparation of optically active alcohols represented by the general formula 2 and their esters represented by the general formula 3 in scheme 1. In more detail, this invention relates to the process for the preparation of optically active alcohols and...

20060234363 - Synthesis of 1,2,4-butanetriol enantiomers from carbohydrates: A bioengineered synthesis scheme for the production of L-1,2,4-butanetriol, D-1,2,4-butanetriol and racemic mixtures thereof from a carbon source is provided. Methods of producing L-1,2,4-butanetriol, D-1,2,4-butanetriol and racemic mixtures thereof are also provided. Methods are also provided for converting D-1,2,4-butanetriol and L-1,2,4,-butanetriol to D,L-1,2,4-butanetriol trinitrate....

20060234364 - Genetically modified yeast species and fermenation processes using genetically modified yeast: Yeast cells are transformed with an exogenous xylose isomerase gene. Additional genetic modifications enhance the ability of the transformed cells to ferment xylose to ethanol or other desired fermentation products. Those modifications', include deletion of non-specific or specific aldose reductase gene(s), deletion of xylitol dehydrogenase gene(s) and/or overexpression of xylulokinase....

20060234366 - Crosslinked enzyme aggregates: Described is a method for the preparation of crosslinked enzyme aggregates, comprising enzyme molecules being associated with an enzyme activity-enhancing agent, the enzyme activity-enhancing agent interacting with the said enzyme molecules, comprising the steps of: A—providing a plurality of enzyme molecules, B—aggregating the enzyme molecules in a liquid medium, by...

20060234365 - Methods of purification of cytochrome p450 proteins: The invention provides a method for the purification of cytochrome P450 molecules, the method comprising expressing in a host cell culture a cytochrome P450 molecule; recovering said cells from said culture and suspending said cells in a high salt buffer; lysing said cells and removing cell debris to provide a...

20060234367 - Methods and composition for the production of orthogonal trna-aminoacyltrna synthetase pairs: This invention provides compositions and methods for generating components of protein biosynthetic machinery including orthogonal tRNAs, orthogonal aminoacyl-tRNA synthetases, and orthogonal pairs of tRNAs/synthetases. Methods for identifying orthogonal pairs are also provided. These components can be used to incorporate unnatural amino acids into proteins in vivo....

20060234368 - Alga-origin promoter, intron and terminator: A promoter, an intron and a terminator by which a useful foreign gene can be efficiently expressed in algae and which are thus useful in breeding algae using genetic engineering techniques....

20060234369 - Implantable biosensor: The present invention provides implantable biosensors. The biosensors comprise tissue or cells which are electrically excitable or are capable of differentiating into electrically excitable cells, and which can be used to monitor the presence or level of a molecule in a physiological fluid. In one embodiment, the tissue or cells...

20060234370 - Analysis and culture apparatus: An analysis and culture apparatus including several wells, which have a cover that closes the well to form a closed space, as well as at least one inlet passage and one outlet passage for introducing matter to a closed space and for removing it from a closed space, respectively. The...

20060234371 - System and method for processing large number of biological microarrays: A system and method for processing biological sensors. The system includes a support component configured to support a fluidic component. The fluidic component includes at least a first container and a second container. The first container is capable of holding a first volume of a first fluid, and the second...

20060234372 - Cell culture method and apparatus for mechanically stimulating cells: A cell culture assembly and a method for culturing cells that provide mechanical stimulation to cells. The cell culture assembly can include a flow chamber positioned in a fluid path and a support comprising cells positioned within the flow chamber to expose the cells to the fluid path. The cell...

20060234374 - Malignant cells and method for selecting the same: The present invention is a method for selecting an enriched population of malignant cells. It has been found that by depleting fibroblasts from a population of cells obtained from a tissue sample; selecting epithelial cells from the fibroblast-depleted population; and culturing the selected epithelial cell population in the presence of...

20060234373 - Treatment for diabetes: Proliferating pancreatic islet cells obtained by the method of isolating a population of cells that preferably includes predominantly islet precursor cells that express one or more marker associated with an islet precursor cell and providing the precursor cells with one or more a pancreatic differentiation agent so that a population...

20060234376 - Repair and regeneration of ocular tissue using postpartum-derived cells: Cells derived from postpartum umbilicus and placenta are disclosed. Pharmaceutical compositions, devices and methods for the regeneration or repair of ocular tissue using the postpartum-derived cells are also disclosed....

20060234375 - Use of human stem cells and/or factors they produce to promote adult mammalian cardiac repair through cardiomyocyte cell division: A method for treating a subject afflicted with a cardiac disorder, in vivo, comprising (i) producing a solution comprising media conditioned from the culture of cells, in vitro, and (ii) administering the solution of step (i) to the subject, thereby treating the cardiac disorder in the subject. Methods for determining...

20060234377 - Cell sheets for ectocornea formation, method of producing the same and method of using the same: The present invention has as its objective providing acorneal epithelium forming cell sheet that will adhere well to an anterior segment tissue. To attain this objective, a corneal epithelium forming cell sheet is produced by a process comprising the steps of cultivating under specified conditions corneal epithelium forming cells on...

20060234378 - Molecular switch regulating neurogenesis: The present disclosure relates to a novel transcription regulatory master switch involved in neural development. The disclosure provides compositions and methods for regulating neural lineage specific genes and modulating the differentiation of stem cells into neural lineage cells....

20060234379 - Cell separation method using hydrophobic solid supports: Provided is a method of separating cells using a hydrophobic solid support. The method comprises contacting a solution containing cells with a hydrophobic solid support having a water contact angle between 70 and 90 degrees. By allowing cells to be adsorbed to the hydrophobic solid substrate, the cells can be...

20060228693 - Composition and method for in vitro preservation of corneal tissues: A corneal storage solution which maintains cell density and cell viability of corneas stored in vitro for a period of greater than four days up to about two weeks is provided. The solution contains a buffered, balanced, nutrient and electrolyte aqueous solution, at least one colloidal osmotic agent, a phenolic...

20060228694 - Methods to individualize combination therapy: Compositions which comprise delivery vehicles having stably associated therewith predetermined, patient-specific combinations of two or more agents, such as antineoplastic agents, are useful in achieving optimal therapeutic effects on a patient-by-patient basis when combinations of drugs are administered....

20060228695 - Predicting hepatotoxicity using cell based assays: Cell based assays are used to assess the hepatotoxicity of a stimulus. Imaging technologies are used to analyze the effects of a stimulus on hepatocytes. Image analysis may characterize the stimulus on the basis of whether it is hepatotoxic, and if so what type of pathology is exhibited; e.g., apoptosis,...

20060228701 - Arrays of protein-capture agents and methods of use thereof: Arrays of protein-capture agents useful for the simultaneous detection of a plurality of proteins which are the expression products, or fragments thereof, of a cell or population of cells in an organism are provided. A variety of antibody arrays, in particular, are described. Methods of both making and using the...

20060228697 - Method to confer cell culture replication activity to different hepatitis c virus isolates: The present invention features methods for producing HCV replicons using HCV encoding sequences from different isolates. The featured methods are based on the discovered importance of NS3 amino acid position 470 in conferring cell culture replication activity to different HCV isolates....

20060228696 - Methods for detecting and inactivating a prion: A method for the isolation or detection of a prion in a sample is disclosed. Also disclosed are methods for the disinfection and/or decontamination and/or inactivation of TSE infectivity....

20060228700 - Novel retrovirus associated with myelodysplastic syndromes and uses thereof: The present invention relates first, to the identification of a novel retrovirus and the novel nucleotide sequences encoding a retroviral polymerase gene (POL nucleotides) associated with the existence of MDS and MDS associated disorders. The present invention further relates to methods for using the MDS associated retroviral nucleotides for the...

20060228699 - Nucleic acid sequences that can be used as primers and probes in the amplification and detection of all subtypes of hiv-1: The present invention is related to nucleic acid sequences that can be used in the field of virus diagnostics, more specifically the diagnosis of infections with the AIDS causing Human Immuno-deficiency Virus (HIV). With the present invention nucleotide sequences are provided that can be used as primers and probes in...

20060228698 - Oligonucleotide for detection of hiv-1 and detection method: An oligonucleotide useful for detection of an RNA derived from HIV-1 consisting of at least 10 consecutive bases in any of SEQ ID NOS:1, 2, 4 to 10 and 13 to 17, which can bind to a specific site of the RNA....

20060228702 - Polynucleotide probe and primer derived from hepatitis e virus recovered from japanese, chip including the same, kit including the same, and method of detecting hepatitis e virus genome using the same: A polynucleotide probe that has a sequence of at least 8 nucleotides and is used for detecting at least one hepatitis E virus selected from JSN-FH, JKN-Sap, JMY-Haw, JAK-Sai, and JRA1; a probe assay kit that includes the polynucleotide probe; and a chip on which the polynucleotide probe has been...

20060228736 - Blood testing method, nucleic acid extraction method, and nucleic acid extraction device: The present invention provides a method which makes it possible to extract both a liquid sample for a biochemical examination and an immunological test and a blood cell sample for a nucleic acid test from one blood sample. Isolation of blood cells is carried out for the blood obtained by...

20060228705 - Cancer-linked gene as target for chemotherapy: Cancer-linked gene sequences, and derived amino acid sequences, are disclosed along with processes for assaying potential antitumor agents based on their modulation of the expression of these cancer-linked genes. Also disclosed are antibodies that react with the disclosed polypeptides and methods of using the antibodies to treat cancerous conditions, such...

20060228751 - Cloning and characterization of the broad-spectrum resistance gene p12: Compositions and methods for enhancing or creating plant disease resistance to plant pests are provided. Transforming a plant with a novel rice Pi2-like disease resistance gene of the invention enhances disease resistance of the plant. Transformed plants, plant cells, tissues, and seed having enhanced disease resistance are also provided....

20060228729 - Comparative analysis of extracellular rna species: The invention provides methods, reagents and kits for enabling comparative analysis of extracellular RNA species in bodily fluids including plasma and serum to detect, infer, or monitor cancer and other neoplasia....

20060228746 - Composition and method for optimized hybridization using modified solutions: The invention provides a composition, kit and method for hybridizing a probe and target at a temperature lower than their standard hybridization temperature. The chemical component added to the composition has a formula R(NH2)C═O, where R is amino or alkyl. A method for use of the chemical component and composition...

20060228724 - Compositions and methods for detecting and treating motor neuron disorders: The present invention relates to the NTE proteins and nucleic acids encoding the NTE proteins. The present invention further provides assays for the detection of NTE polymorphisms and mutations associated with disease states, as well as methods of screening for ligands and modulators of NTE proteins....

20060228731 - Compositions and methods of using a synthetic dnase i: Compositions and method for making and using a synthetic bovine DNase I are disclosed. More particularly, the sbDNase I of the present invention is a versatile enzyme that cleaves DNA nonspecifically to release 5′-phosphorylated nucleotides. The sbDNase I molecules of the present invention find particular use in a wide range...

20060228742 - Correcting an assay image of an array of signals generated from a multiplexed hybridization-mediated assay: Described are methods of assay design and assay image correction, useful for multiplexed genetic screening for mutations and polymorphisms, including CF-related mutants and polymorphs, using an array of probe pairs (in one aspect, where one member is complementary to a particular mutant or polymorphic allele and the other member is...

20060228743 - Correcting for false positive signals from mismatched probe-target binding in a multiplexed hybridization-mediated assay: Described are methods of assay design and assay image correction, useful for multiplexed genetic screening for mutations and polymorphisms, including CF-related mutants and polymorphs, using an array of probe pairs (in one aspect, where one member is complementary to a particular mutant or polymorphic allele and the other member is...

20060228713 - Detection of single nucleotide polymorphisms using planar waveguides: Methods and apparatus for detecting single nucleotide polymorphisms in genes of interest are disclosed. A plurality of probes is immobilized on a planar waveguide. The probes comprise sequences complementary to a wildtype sequence of the gene of interest and complementary to a sequence of a known SNP in the gene...

20060228744 - Differentiating homozygous, heterozygous and wild-type alleles using a multiplexed hybridization-mediated assay: Described are methods of assay design and assay image correction, useful for multiplexed genetic screening for mutations and polymorphisms, including CF-related mutants and polymorphs, using an array of probe pairs (in one aspect, where one member is complementary to a particular mutant or polymorphic allele and the other member is...

20060228703 - Displacement assay for the detection of nucleic acid oligomer hybridization events: Described is a method for detection of nucleic acid oligomer hybridization events, the method comprising the steps: providing a modified surface, the modification consisting in the attachment of at least one type of ligate nucleic acid oligomer; providing signal nucleic acid oligomer ligands; providing a sample having nucleic acid oligomer...

20060228738 - Dna-polypyrrole based biosensors for rapid detection of microorganisms: A DNA-polypyrrole based biosensor and methods of using the biosensor for the rapid detection of Escherichia Coli and other microorganisms. The DNA-polypyrrole biosensor can be used to detect dangerous micoorganisms for monitoring water quality of a sample from a drinking water or food source. The biosensor can use genomic DNA...

20060228707 - Eukaryotic genes involved in adult lifespan regulation: The present invention relates to regulation of lifespan in eukaryotes. More particularly, one aspect of the present invention is the identification of genes, gene products, and genes in pathways controlled by such genes and gene products, using RNAi and microarray analysis, that regulate lifespan (e.g., extend or truncate adult lifespan)...

20060228734 - Fluid processing device with captured reagent beads: A fluid processing device, method and system are provided. The fluid processing device can comprise: a substrate; a plurality of reaction regions disposed in or on the substrate; at least one channel interconnecting the plurality of reaction regions, the at least one channel having a cross-sectional area that includes a...

20060228745 - Gene detection assay for improving the likelhood of an effective response to an erbb antagonist cancer therapy: The invention provides a method for more effective treatment of patients susceptible to or diagnosed with tumors overexpressing ErbB, as determined by a gene amplification assay, with an ErbB antagonist. Such method comprises administering a cancer-treating dose of the ErbB antagonist, preferably in addition to chemotherapeutic agents, to a subject...

20060228728 - Genetic basis of alzheimer's disease and diagnosis and treatment thereof: A collection of polymorphic sites having resistance or susceptibility to Alzheimer's disease is provided. The sites are useful in methods of diagnosing and treating Alzheimer's disease and related conditions....

20060228715 - Genetic polymorphisms associated with coronary heart disease, methods of detection and uses thereof: The present invention is based on the discovery of genetic polymorphisms that are associated with coronary heart disease, and in particular MI, and response to drug treatment. In particular, the present invention relates to nucleic acid molecules containing the polymorphisms, variant proteins encoded by such nucleic acid molecules, reagents for...

20060228752 - Genetic polymorphisms in the preprotachykinin gene: The present invention relates to a method for correlating single nucleotide polymorphisms in the preprotachykinin (NKNA) gene with the efficacy and compatibility of a pharmaceutically active compound administered to a human being. The invention further relates to a method for determining the efficacy and compatibility of a pharmaceutically active compound...

20060228748 - Hybrid phthalocyanine derivatives and their uses: Water soluble hybrid phthalocyanine derivatives useful in competitive and noncompetitive assays immunoassays, nucleic acid and assays are disclosed and claimed having (1) at least one donor subunit with a desired excitation peak, and (2) at least one acceptor subunit with a desired emission peak, wherein said derivative(s) is/are capable of...

20060228733 - Hybridization chain reaction amplification for in situ imaging: The present invention relates to the use of fluorescently labeled nucleic acid probes to identify and image analytes in a biological sample. In the preferred embodiments, a probe is provided that comprises a target region able to specifically bind an analyte of interest and an initiator region that is able...

20060228739 - Light-controlled electrokinetic assembly of particles near surfaces: A method and apparatus for the manipulation of colloidal particles and biomolecules at the interface between an insulating electrode such as silicon oxide and an electrolyte solution. Light-controlled electrokinetic assembly of particles near surfaces relies on the combination of three functional elements: the AC electric field-induced assembly of planar aggregates;...

20060228740 - Light-controlled electrokinetic assembly of particles near surfaces: A method and apparatus for the manipulation of colloidal particles and biomolecules at the interface between an insulating electrode such as silicon oxide and an electrolyte solution. Light-controlled electrokinetic assembly of particles near surfaces relies on the combination of three functional elements: the AC electric field-induced assembly of planar aggregates;...

20060228741 - Light-controlled electrokinetic assembly of particles near surfaces: A method and apparatus for the manipulation of colloidal particles and biomolecules at the interface between an insulating electrode such as silicon oxide and an electrolyte solution. Light-controlled electrokinetic assembly of particles near surfaces relies on the combination of three functional elements: the AC electric field-induced assembly of planar aggregates;...

20060228732 - Method enabling the use of extracellular ribonucleic acid (rna) extracted from plasma of serum to detect, monitor or evaluate cancer or premalignant conditions: This invention relates to the use of tumor-derived or associated extracellular ribonucleic acid (RNA) found circulating in the plasma or serum fraction of blood for the detection, monitoring, or evaluation of cancer or premalignant conditions. Extracellular RNA may circulate as non-bound RNA, protein-bound RNA, lipid-RNA complexes, lipoprotein (proteolipid)—RNA complexes, protein-RNA...

20060228720 - Method for imaging an array of microspheres: A method for imaging an array of microspheres. A light source, filter or combination of filters that isolate wavelength, or wavelength ranges, of light and a camera are used for detecting and quantifying the presence of biological probes that indicate the presence of specific chemical moieties within a biological system....

20060228719 - Method for imaging an array of microspheres using specular illumination: A specular light source and camera for detecting and quantifying the presence of biological probes, which contain a colorant with spectral properties that depend upon the angle of illumination and angle of imaging, that indicate the presence of specific chemical moieties within a biological system....

20060228718 - Method for rapidly identifying bacteria and kit thereof: The present invention provides a method for identifying bacteria in a sample comprising (a) obtaining the intergenic spacer region separating the 16S and 23S rDNA of bacteria in the sample; and (b) hybridizing the intergenic spacer region with at least one specific probe. A kit for identifying bacteria in a...

20060228727 - Method for the detection of cancer: The present invention relates to a method for the diagnosis and/or the follow up of the evolution of cancer, which includes the analysis and quantification of over expressed and amplified genes in the plasma/serum of cancer patients or persons suspected to harbor cancer. This is achieved by analyzing together the...

20060228737 - Method of isolating a nucleic acid using a material containing an amino group and a carboxyl group and positively charged at a first ph and a solid material for nucleic acid isolation used for the method: Provided are a method of isolating a nucleic acid from a sample and a solid material for isolating the nucleic acid which can be used for the above method. The method includes contacting the sample with a bifunctional material containing an amino group and a carboxyl group at a first...

20060228750 - Method to determine the risk for side effects of an ssri treatment in a person: A new method is found to determine an increased risk for side effects of an SSRI treatment in a person by genotyping the person for the presence of the 102 C/C DNA sequence in the 5-HT2A receptor gene. This provides for a method to improve the treatment of an SSRI...

20060228725 - Method using a nonlinear optical technique for detection of interactions involving a conformational change: A nonlinear optical technique, such as second or third harmonic or sum or difference frequency generation, is used to detect binding interactions, or the degree or extent of binding, that comprise a conformational change. In one aspect of the present invention, the nonlinear optical technique detects a conformational change in...

20060228721 - Methods for determining sequence variants using ultra-deep sequencing: The claimed invention provides for new sample preparation methods enabling direct sequencing of PCR products using pyrophosphate sequencing techniques. The PCR products may be specific regions of a genome. The techniques provided in this disclosure allows for SNP (single nucleotide polymorphism) detection, classification, and assessment of individual allelic polymorphisms in...

20060228730 - Methods for identifying small molecules that bind specific rna structural motifs: The present invention relates to a method for screening and identifying test compounds that bind to a preselected target ribonucleic acid (“RNA”). Direct, non-competitive binding assays are advantageously used to screen libraries of compounds for those that selectively bind to a preselected target RNA. Binding of target RNA molecules to...

20060228749 - Methods for manipulating moieties in microfluidic systems: This invention relates generally to the field of moiety or molecule manipulation in a chip format. In particular, the invention provides a method for manipulating a moiety in a microfluidic application, which method comprises: a) coupling a moiety to be manipulated onto surface of a binding partner of said moiety...

20060228706 - Methods of diagnosing and treating diabetes and insulin resistance: The present invention provides compositions and methods for diagnosing and treating diabetes and insulin resistance. In particular, the invention provides methods of identifying modulators of the polynucleotides or polypeptides of the invention and using those modulators to treat diabetes, as well as methods of diagnosing diabetes by measuring the levels...

20060228717 - Microfluidic system and method of utilization: Systems and methods used for isolating and analyzing biological material. More particularly, microfluidic devices including chemical array(s) and detection systems are used for analyzing biological material....

20060228735 - Multiplex assay systems: The methods described herein can be used, e.g., for detecting at least two target molecules in a sample (e.g., a single sample). Exemplary methods include using a target-specific switch probe attached to a substrate and using zip codes to associate holographically encoded particles with a target-specific probe....

20060228709 - Novel fibulin-like polypeptides: The present invention discloses open reading frames (ORFs) in human genome encoding for novel fibulin-like polypeptides, and reagents related thereto including variants, mutants and fragments of said polypeptides, as well as ligands and antagonists directed against them. The invention provides methods for identifying and making these molecules, for preparing pharmaceutical...

20060228712 - Novel polynucleotides: Novel polynucleotides derived from microorganisms belonging to coryneform bacteria and fragments thereof, polypeptides encoded by the polynucleotides and fragments thereof, polynucleotide arrays comprising the polynucleotides and fragments thereof, recording media in which the nucleotide sequences of the polynucleotide and fragments thereof have been recorded which are readable in a computer,...

20060228710 - Novel therapeutic targets in cancer: The present invention relates to novel sequences for use in detection, diagnosis and treatment of cancers, especially lymphomas. The invention provides cancer-associated (CA) polynucleotide sequences whose expression is associated with cancer. The present invention provides CA polypeptides associated with cancer that are present on the cell surface and present novel...

20060228714 - Nucleic acid representations utilizing type iib restriction endonuclease cleavage products: This invention encompasses nucleic acid libraries comprising Type IIB restriction endonuclease cleavage products, or tags, including concatenated tags, and using concatenated and single Type IIB restriction endonuclease cleavage tags in diverse methods including karyotyping, pathogen discovery, identification of novel genes, subtraction techniques and transcript profiling....

20060228726 - Polypeptides having nucleic acid binding activity and compositions and methods for nucleic acid amplification: Polypeptides having nucleic acid binding activity are provided. Methods of using polypeptides having nucleic acid binding activity are provided. Fusion proteins and methods of using fusion proteins are provided. Fusion proteins comprising a polymerase and a nucleic acid binding polypeptide are provided. Fusion proteins comprising a reverse transcriptase and a...

20060228711 - Probe carrier and method for quantifying target substance using the probe carrier: A probe carrier is provided on which probes capable of specifically binding to a target substance are immobilized as a plurality of spots in known locations on the carrier, characterized in that the probe carrier has two or more separated areas, wherein in each area probes of the same kind...

20060228708 - Protein synthesis monitoring (psm): A method and a device are disclosed for monitoring the synthesis of proteins by the ribosome in real time, in vivo as well as in in-vitro. The ribosome is engineered to carry a donor fluorophore, and tRNA and/or amino acids and/or some other part of the ribosome are either engineered...

20060228723 - System and method for electronic sensing of biomolecules: A nanoelectronic device is combined with a cellular membrane component to provide a sensor for biomolecules or to provide information about the structure of the membrane. The nanoelectronic device may comprise a network of randomly-oriented nanotubes, or other nanostructure, arranged on a substrate with adjacent electrodes so as to operate...

20060228747 - Systems and methods for detecting and analyzing polymers: A detection system and methods for improving the ability of the detection system to recognize labels that are disposed on a polymer. Embodiments of the invention include schemes for selecting emitters and labels used within the system in a manner that allows an increase in the number of distinct labels...

20060228716 - Thin film coated microwell arrays and methods of making same: The invention relates to a thin film coated array and the process of fabricating and using the array including methods of etching, depositing a thin film coating, preparing and using the thin film coated array....

20060228722 - Thin film coated microwell arrays and methods of making same: This invention relates to microwell array compositions which are coated with one or more thin film coatings. The invention includes the process of fabricating and using thin film coated microwell arrays....

20060228704 - Visualization of introduced dna (void) in transit by in situ hybridization: The invention relates to a process for monitoring exogenous nucleic acid in transit, termed Visualization Of Introduced DNA (VOID). Once the nucleic acid has been introduced into a cell in a biological sample, the cells are fixed and permeabilized if necessary, then subjected to an in situ hybridization procedure in...

20060228760 - Allergen detection apparatus: A method of determining allergen activity in dust comprises: providing a dust sample; extracting from the dust sample at least one breakdown component or proteins or peptides; reacting the extracted at least one breakdown component with a colorimetric amine detection reagent such as TNBSA; and quantitatively measuring the intensity of...

20060228758 - Analysis of mhc-peptide binding interactions: Methods, apparatuses, and compounds for screening or detecting binding of candidate peptides to an MHC protein is provided. A first component including at least one candidate peptides and a second component including at least one MHC protein are contacted. One of the components is immobilized on a solid support. The...

20060228759 - Analysis of mhc-peptide binding interactions: Methods, apparatuses, and compounds for screening or detecting binding of candidate peptides to an MHC protein is provided. A first component including at least one candidate peptides and a second component including at least one MHC protein are contacted. One of the components is immobilized on a solid support. The...

20060228757 - Db, the receptor for leptin, nucleic acids encoding the receptor, and uses thereof: The present invention relates to identification of a receptor for a satiety factor, which is involved in body weight homeostasis. Mutations in this receptor are associated with obese phenotypes. In particular, the present invention relates to identification and characterization of the receptor for leptin, including a naturally occurring soluble form...

20060228755 - Ectopic pregnancy markers: The present invention relates to methods for determining a risk or identifying a condition associated with the presence of ectopic pregnancy in a subject as well as testing a candidate compound for a therapeutic activity against an ectopic pregnancy and sorting patients based on the risk of having ectopic pregnancy....

20060228754 - Methods for screening compound libraries: Disclosed are methods for screening compound libraries using frontal chromatography in combination with mass spectrometry to identify and rank those members of the library that bind to a target receptor. Methods are also disclosed which permit a compound library to be rapidly screened to determine if any member of the...

20060228753 - Methods of detecting a phenotype of a polymorphic protein: The present invention provides an antibody that differentially reacts with allelic variants of a polymorphic protein, methods of identifying same, an antigen binding fragment comprised therein, nucleic acids encoding same, proteins, cells, viral particles, compositions, and kits comprising same. The invention also provides methods for determining a haptoglobin type of...

20060228756 - Relational database management system for automated random crystallization screening: A relational database management system for automated random crystallization screening systems so as to provide facilitated data tracking, maintenance, and analysis. The system includes a database server module capable of storing data; an ARCS module having a crystallization screen design engine capable of generating random crystallization screens and associated crystallization...

20060228761 - Novel rip3 associated cell cycle proteins, compositions and methods of use: The present invention is directed to novel polypeptides, nucleic acids and related molecules which have an effect on or are related to the cell cycle. Also provided herein are vectors and host cells comprising those nucleic acid sequences, chimeric polypeptide molecules comprising the polypeptides of the present invention fused to...

20060228762 - Diagnosis of human glycosylation disorders: This invention provides methods and kits for use in diagnosing genetically transmitted diseases that are associated with deficiencies in glycosylation of glycoconjugates such as glycoproteins, glycolipids, and proteoglycans. The methods and kits are also useful for monitoring the course of treatment of diseases that are associated with glycosylation disorders....

20060228763 - Periostin-based diagnostic assays: The invention includes novel human periostin polypeptides and DNAs encoding them. Also embraced by the invention are human periostin specific antibodies, diagnostic assays for metastasis of breast cancer to bone, and preeclempsia....

20060228764 - Fluoresence assay for mtp activity: The present invention is directed to methods for assaying microsomal triglyceride transfer protein (MTP) which are amenable to automation and high-throughput screening. The assays may be used to measure MTP activity in cell and tissue homogenates as well as purified MTP. The methods provided by the present invention have the...

20060228765 - Method for detecting procoagulant phospholipid: The present invention relates to a method for determining the amount of procoagulant phospholipid in a sample, said method comprising steps (i) to (iii) performed in the following order: (i) forming an admixture of the sample and a substrate plasma which has been rendered free or substantially free of procoagulant...

20060228766 - Assay for detection of transferase enzyme activity in drug screening: The invention provides methods for assaying the activity of the translocase enzyme and/or transferase enzyme involved in peptidoglycan biosynthesis in bacteria using scintillation proximity assay methodology. The methods are suitable for high throughput screening of potential anti-bacterial drugs....

20060228767 - Test strip for determining concentration of creatinine in a body fluid sample: Apparatus for determining concentration of creatinine in whole blood or plasma using a 1-methylhydantoinase (NMHase) catalyzed reaction, wherein the invention uses commercially available NMHase and thus eliminates prior art stabilization procedures for NMHase. Conveniently, known Trinder reagents and oxidative couplers are used for the indicator system. It has been found...

20060228768 - Method of judging the onset of periodontitis: The inventor discovered that γ-GTP (γ-glutamyl transpeptidase) was scarcely contained in the gingival crevicular fluid of patients with gingivitis, but was contained in the gingival crevicular fluid of patients with periodontitis. Thus, the inventor provides a determination method of development of periodontitis by detecting or measuring γ-GTP contained in the...

20060228770 - Evaluation system for drug-metabolizing capacity and use thereof: The amount of drug consumption caused by a specific drug-metabolizing enzyme and/or the amount of the resulting metabolite(s) is measured by chromatography with an aqueous mobile phase under temperature control using a packing material whose surface is coated with a polymer having a hydration force varying in the temperature range...

20060228769 - Method of detecting allergen protein: The present invention relates to a method for detecting a protein having a disulfide bond or an allergen protein, comprising: protecting by chemically modifying a free SH group of a protein in a sample to be tested; cleaving a disulfide bond of the free SH group-protected protein to expose SH...

20060228771 - Apparatus for and method of making electrical measurements on objects: This invention relates to an apparatus for, and method of, making electrical measurements on objects, in particular cells, liposomes or similar small objects, in a medium. More particularly the invention relates to an apparatus for, and method of, making electrophysiological measurements on cells, liposomes or similar small objects, in a...

20060228772 - Compositions and methods for preparing specimens for microscopic analysis: Compositions and methods for preparing a biological specimen for microscopic analysis. Fixing compositions and dewatering compositions are provided with methods for their use that improve sample preparation and result in greater detail being available in the prepared specimen. Such compositions and methods also render more quantitative analysis of prepared specimens...

20060228777 - Animal product free media and processes for obtaining a botulinum toxin: Media and processes for the fermentation of Clostridium botulinum and obtaining a botulinum toxin for use in formulating botulinum toxin pharmaceutical compositions. The growth media can contain significantly reduced levels of meat or dairy by-products using non-animal based products to replace the animal-derived products. Preferably, the media used are substantially...

20060228780 - Chromatographic method and system for purifying a botulinum toxin: Chromatographic processes and systems for purifying a botulinum toxin from an APF fermentation medium....

20060228775 - Differential expression of nucleic acid molecules: The present invention relates generally to nucleic acid molecules expressed at least in the hypothalamus, liver, mesenteric adipose tissue, or red gastrocnemius muscle conveniently identified using differential display techniques under differing physiological conditions. The nucleic acid molecules are associated with or act as markers for conditions of inter alia a...

20060228773 - Method for preparing recombinant heterocarpine: A process for preparing recombinant heterocarpine with the complete sequence of heterocarpine (SEQ. ID. NO. 10), expression vectors comprising a polynucleotide encoding for heterocarpine, host cells transformed or transfected by said expression vectors as well as a process for obtaining heterocarpine by means of said transformed or transfected host cells...

20060228779 - Method for producing factor g derived from horseshoe crab: The invention provides a virus harboring a DNA encoding a subunit of limulus-derived factor G, the virus being capable of mass-producing a (1→3)-β-D-glucan assay reagent of satisfactory quality, steadily and at low cost, a cell harboring the virus, and a method of producing factor G by use of the cell....

20060228778 - Novel vitamin d receptor related polypeptides, nucleic acid sequence encoding the same and uses thereof: The present invention relates to novel vitamin D receptor related (VDRR) polypeptides, and formulations containing the same. Nucleic acid sequences encoding the VDRR polypeptides, expression vectors containing such sequences and host cells transformed with such expression vectors are also disclosed, as are methods for the expression of the novel VDRR...

20060228774 - Oxygen-resistant hydrogenases and methods for designing and making same: The invention provides oxygen-resistant iron-hydrogenases ([Fe]-hydrogenases) for use in the production of H2. Methods used in the design and engineering of the oxygen-resistant [Fe]-hydrogenases are disclosed, as are the methods of transforming and culturing appropriate host cells with the oxygen-resistant [Fe]-hyrdogenases. Finally, the invention provides methods for utilizing the transformed,...

20060228776 - Pink-1 promoter: The invention provides methods and compositions of an upstream regulatory element (PINK-1 promoter) operably linked to an expressible gene, wherein the expression of the expressible gene is driven by the upstream regulatory element....

20060228781 - Synthetic gene coding for human granulocyte-colony stimulating factor for the expression in e.coli: The invention relates to the synthetic gene coding for hG-CSF which enables expression in E. coli with an improved expression level of the recombinant hG-CSF regarding the total cellular proteins after expression....

20060228783 - Expression vectors, transformed host cells and fermentation process for the production of recombinant polypeptides: The present invention relates to an expression vector, comprising a polynucleotide encoding a fusion protein comprising the signal sequence of the gac gene of Pseudomonas diminuta and a polypeptide of interest, a prokaryotic host cell transformed with such an expression vector and a process for production of a polypeptide of...

20060228782 - Factor vii or viia - like molecules: Conjugates of Factor VII (FVII) and Factor VIIa (FVIIA) are provided, as are methods for preparing them. Methods for producing novel polypeptides contributing to the production of such conjugates are provided. Methods of treatment by administering a FVII or FVIIa conjugate are provided....

20060228784 - Sugar chain asparagine derivatives, sugar chain asparagine, sugar chain, and processes for producing these: An asparagine-linked α2,3-oligosaccharide having undeca- to hepta-saccharides, an asparagine-linked α2,6-oligosaccharide having undeca- to hepta-saccharides and containing fluorine and an asparagine-linked oligosaccharide derivative containing at least one fucose in N-acetylglucosamine on the nonreducing terminal side of an asparagine-linked oligosaccharide wherein the asparagine has amino group protected with a lipophilic protective group,...

20060228785 - Recombinant microorganism for the production of vitamin b6: Disclosed is a recombinant microorganism being capable of producing vitamin B6, wherein said microorganism carries extra genes which code for an enzyme combination selected from: i) erythrose 4-phosphate dehydrogenase and 1-deoxy-D-xylulose 5-phosphate synthase; ii) erythrose 4-phosphate dehydrogenase and pyridoxol 5′-phosphate synthase; and iii) erythrose 4-phosphate dehydrogenase, 1-deoxy-D-xylulose 5-phosphate synthase and...

20060228786 - Polymerase-based protocols for the introduction of deletions and insertions: The present invention relates to improved methods of introducing site-directed mutations into circular DNA without the need for subcloning. The invention comprises single-stage, polymerase-based, mutagenesis procedures which eliminate the need for the second-stage linear cyclic amplification reaction of the prior art. In accordance with the invention, production of complimentary mutagenized...

20060228787 - Mutants for the preparation of d-amino acids: The present invention relates to specific E. coli mutants which can be used for the synthesis of D-amino acids, and to such a process. The mutants are distinguished by deficiencies in particular enzymes which break down D-amino acids....

20060228788 - Thermostable omega-transaminases: Thermostable omega-transaminases, particularly thermostable omega-transaminases which have a high reaction rate and which are tolerant to high concentrations of donor amine, can be used to enrich enantiomerically a mixture of chiral amines or to synthesize stereoselectively one of a pair of chiral amines in which the amino group is bound...

20060228789 - Xylose-fermenting recombinant yeast strains: Disclosed are xylose-fermenting recombinant yeast strains expressing xylose reductase, xylitol dehydrogenase, and xylulokinase and having reduced expression of PHO13 or a PHO13 ortholog, as well as methods of fermenting xylose to obtain ethanol using the recombinant yeast strains....

20060228790 - L-glutamate oxidase: (B) substrate specificity: being specific to L-glutamic acid; (C) molecular weight and subunit structure: molecular weight as determined through SDS-polyacrylamide gel electrophoresis of 70,000±6,000, molecular weight as determined through gel filtration of 140,000±10,000, and being a dimer formed of the same subunits having a molecular weight of 70,000±6,000; (D) optimum...

20060228791 - Subtilases and subtilase variants having altered immunogenicity: The present invention relates to subtilase variants and subtilases with an altered immunogenicity, particularly subtilase variants and subtilases with a reduced allergenecity. Furthermore, the invention relates to expression of said subtilase variants and subtilases and to their use, such as in detergents and oral care products....

20060228792 - Microorganism capable of degrading aromatic polyester and method of degrading aromatic polyester using the same: A microorganism which belongs to the genus Rhizobium and has the ability of degrading an aromatic polyester, and a method of degrading an aromatic polyester by using the microorganism. According to this method, the aromatic polyester can be degraded safely and relatively swiftly at a low cost....

20060228793 - Microsystem for separating serum from blood: A microsystem for separating serum from blood using a centrifuge is provided. The microsystem includes various chambers and channels. When blood is injected into the channels and centrifuged, serum and blood cells in the injected blood are distributed into different chambers. Thus, the serum can be separated from the blood....

20060228794 - Incubation and/or stroage container system and method: A container assembly (10) comprises a vessel (21) for containing a biological medium, gametes and/or one or more embryo(s). The vessel (21) has a CO2 permeable seal (28) and a closure device (30) for selective access. A buffer chamber (60) for a CO2 enriched atmosphere at least partially surrounds the...

20060228796 - Adipose tissue-derived adult stem cells for the repair of articular cartilage fractures and uses thereof: The invention provides cells, methods and compositions based upon the use of adipose tissue-derived adult stem cells in the repair of articular cartilage fractures or defects. The invention is useful in providing a treatment of articular cartilage fractures in a clinical setting....

20060228795 - Apparatus for enhancing proliferation of cells in a small-scale cell culturing container: An interchangeable sleeve for enhancing proliferation of cells in a small-scale culturing container is disclosed with the interchangeable sleeve having a substantially cylindrical and substantially rigid electrical conductive material wound in a cylindrical shape and capable of being connected to a pulsating time varying electromagnetic current to create a time...

20060228797 - Method for altering the metabolism of plant: The invention is related to a method of altering the metabolism of a plant by carrying out the steps of treating seeds of the plant with non-genetically engineered selected mutant pink-pigmented facultative methylotroph having a plant altering capability, and growing the plants from the treated seeds, wherein the plants that...

20060228798 - Homologous recombination in multipotent adult progenitor cells: The invention relates to methods of altering gene expression by homologous recombination in a multipotent adult progenitor cell (MAPC). In particular, methods of producing a recombinant MAPC, of correcting a genetic defect in a mammal, of providing a functional and/or therapeutic protein to a mammal, and of transforming a MAPC...

20060228800 - Novel transgenic methods using intronic rna: The present invention relates to a method and composition for generating an artificial intron and its components capable of producing microRNA (miRNA) molecules and thus inducing specific gene silencing effects through intracellular RNA interference (RNAi) mechanisms, and the relative utilization thereof. The miRNA-producing intron so generated is not only useful...

20060228799 - Use of consensus sequences for targeted homologous gene isolation and recombination in gene families: The invention relates to compositions and methods for targeting sequence modifications in one or more genes of a related family of genes using enhanced homologous recombination techniques. These techniques may be used to create animal or plant models of disease as well as to identify new targets for drug or...

20060223046 - System and method to simulate hemodynamics: A system for hemodynamic simulation comprises a vessel having properties of a blood vessel, a reservoir containing a quantity of fluid, tubing connecting the vessel and reservoir, and at least one pump for circulating the fluid within the system. Fluid can be tissue culture medium or blood analog fluid, and...

20060223047 - System and method to simulate hemodynamics: A system for hemodynamic simulation comprises a vessel having properties of a blood vessel, a reservoir containing a quantity of fluid, tubing connecting the vessel and reservoir, and at least one pump for circulating the fluid within the system. Fluid can be tissue culture medium or blood analog fluid, and...

20060223048 - System and method to simulate hemodynamics: A system for hemodynamic simulation comprises a vessel having properties of a blood vessel, a reservoir containing a quantity of fluid, tubing connecting the vessel and reservoir, and at least one pump for circulating the fluid within the system. Fluid can be tissue culture medium or blood analog fluid, and...

20060223049 - System and method to simulate hemodynamics: A system for hemodynamic simulation comprises a vessel having properties of a blood vessel, a reservoir containing a quantity of fluid, tubing connecting the vessel and reservoir, and at least one pump for circulating the fluid within the system. Fluid can be tissue culture medium or blood analog fluid, and...

20060223050 - Therapeutic platelets and methods: The invention provides methods for loading a preservative into blood platelets comprising providing a preservative solution having a preservative, water and protein, and loading blood platelets with the preservative solution to produce preservative-loaded blood platelets having the preservative solution generally including higher glass transition temperatures than glass transition temperatures for...

20060223051 - System and method for performing g protein coupled receptor (gpcr) cell assays using waveguide-grating sensors: The present invention includes a system and method that uses optical LID biosensors to monitor in real time agonist-induced GPCR signaling events within living cells. Particularly, the present invention includes a system and method for using an optical LID biosensor to screen compounds against a target GPCR within living cells...

20060223053 - Direct measurement of sorption on three-dimensional surfaces such as resins, membranes or other preformed materials using lateral dispersion to estimate rapid sorption kinetics or high binding capacities: Described are methods allowing measurement of adsorption and desorption of analytes with membranes or resins directly using surface plasmon resonance (SPR). Also described are methods for assembling intact resins or membranes on SPR surfaces. Such methods provide estimates of mass-action ion-exchange adsorption and desorption rates accounting for steric (σ) and...

20060223058 - In vitro association studies: Cell and tissue autonomous phenotypes are correlated with genotype information. Correlated genotype information is used to screen individual traits. Methods and systems for correlating cell and tissue autonomous phenotypes to genotype information are provided....

20060223055 - Methods and compositions for treatment of viral lnfection: The present invention relates to methods and compositions for the treatment and/or prevention of diseases or disorders associated with viral infection. Accordingly, the nucleotide and amino acid sequences for a human Brd4 protein are provided. Also provided are complexes comprising Brd4 and an E2 protein or a functional equivalent of...

20060223054 - Methods for identifying a peptide that binds a geometrical shape: Provided herein are methods, such as phage display assays, for bioengineering peptides that bind to geometrically and/or atomically structured molecular surfaces such as, for example, flat surfaces, smooth curved surfaces, as well as surfaces with periodic, random, or fractal atomic configurations. Surface-binding peptides are provided that are identified using the...

20060223056 - Nucleic acid sequences that can be used as primers and probes in the amplification and detection of all subtypes of hiv-1: The present invention is related to nucleic acid sequences that can be used in the field of virus diagnostics, more specifically the diagnosis of infections with the AIDS causing Human Immuno-deficiency Virus (HIV). With the present invention nucleotide sequences are provided that can be used as primers and probes in...

20060223057 - Nucleic acid sequences that can be used as primers and probes in the amplification and detection of all subtypes of hiv-1: The present invention is related to nucleic acid sequences that can be used in the field of virus diagnostics, more specifically the diagnosis of infections with the AIDS causing Human Immuno-deficiency Virus (HIV). With the present invention nucleotide sequences are provided that can be used as primers and probes in...

20060223052 - Technique for detecting microorganisms: A technique for detecting the presence of microorganisms in a simple, rapid, and efficient manner is provided. More specifically, the technique involves identifying one or more volatile compounds associated with a particular microorganism of interest. The volatile compounds may be identified, for instance, using solid phase microextraction in conjunction with...

20060223077 - 207 human secreted proteins: The present invention relates to novel human secreted proteins and isolated nucleic acids containing the coding regions of the genes encoding such proteins. Also provided are vectors, host cells, antibodies, and recombinant methods for producing human secreted proteins. The invention further relates to diagnostic and therapeutic methods useful for diagnosing...

20060223096 - Antigen binding molecules directed to mcsp and having increased fc receptor binding affinity and effector function: The present invention relates to antigen binding molecules (ABMs). In particular embodiments, the present invention relates to recombinant monoclonal antibodies, including chimeric, primatized or humanized antibodies specific for human MCSP. In addition, the present invention relates to nucleic acid molecules encoding such ABMs, and vectors and host cells comprising such...

20060223092 - Assays, methods and kits for detecting small nuclear ribonucleoprotein particle assembly and survival of motor neurons activity: The present invention includes assays and kits for detecting the assembly of an RNA binding protein-RNA complex and for detecting the activity of an RNA binding protein....

20060223097 - Capturing sequences adjacent to type-iis restriction sites for genomic library mapping: The present invention relates to novel methods for sequencing and mapping genetic markers in polynucleotide sequences using Type-IIs restriction endonucleases. The methods herein described result in the “capturing” and determination of specific oligonucleotide sequences located adjacent to Type-IIs restriction sites. The resulting sequences are useful as effective markers for use...

20060223061 - Centrifugal device and method for performing binding assays: The invention relates to a device for performing binding assays. In particular, the invention relates to a centrifugal device for performing such assays. The invention also relates to a method of performing binding assays involving antigen-antibody binding, nucleic acid hybridization, or receptor-ligand interaction....

20060223098 - Circularizable nucleic acid probes and amplification methods: The present invention relates to methods for preparing linear circularizable nucleic acid probes and/or circular nucleic acid probes. Such probes may be used as the starting material for rolling circle amplification (RCA) and/or ramification-extension amplification (RAM) of nucleic acid molecules. The present invention further provides circular probes and linear circularizable...

20060223080 - Compositions and methods for detecting group a streptococci: Compositions, methods and kits for detecting Group A streptococci. Particularly described are oligonucleotides that are useful as amplification primers and hybridization probes for detecting very low levels of Group A streptococci nucleic acids....

20060223101 - Compositions and methods for screening anti-cancer agents in yeast: Methods and kits for identifying novel anti-tumor agents are provided....

20060223076 - Cyanine dyes and their applications as luminescence quenching compounds: The quenching compounds of the invention are weakly luminescent cyanines that are substituted by one or more heteroaromatic quenching moieties. The quenching compounds of the invention exhibit little or no observable luminescence and efficiently quench a broad spectrum of luminescent compounds. The chemically reactive quenching compounds possess utility for labeling...

20060223107 - Detection of nucleic acid sequences by cleavage and separation of tag-containing structures: The present invention is directed to a method of detecting pluralities of target nucleic acid sequences by forming and cleaving duplex structures with a pair of probes, one probe of each pair being labeled with an electrophoretic tag. Cleavage of the duplex structures releases electrophoretic tags that are then separated...

20060223065 - Diagnostic and therapeutic use of a sulfotransferase for neurodegenerative diseases: The present invention discloses the differential expression of a cytosolic sulfotransferase in specific brain regions of Alzheimer's disease patients. Based on this finding, this invention provides a method for diagnosing or prognosticating a neurodegenerative disease, in particular Alzheimer's disease, in a subject, or for determining whether a subject is at...

20060223106 - Early-stage multipotential stem cells in colonies of bone marrow stromal cells: Marrow stromal cells (MSCs) are adult stem cells from bone marrow that can differentiate into multiple non-hematopoietic cell lineages. Colonies of human MSCs were shown to contain both small, rapidly self-renewing stem cells (RS cells) and large, more mature cells (mMSCs). Samples enriched for RS cells had a greater potential...

20060223079 - Electrocatalytic nucleic acid hybridization detection: The detection of specific DNA sequences using electrochemical readout would permit the rapid and inexpensive detection and identification of bacterial pathogens and the analysis of human genes. A new assay developed for this purpose is described that harnesses an electrocatalytic process to monitor DNA hybridization....

20060223086 - Free reactant use in nucleic acid-templated synthesis: The present invention provides methods and compositions for expanding the scope of chemical reactions that can be performed during nucleic acid-templated organic syntheses. In particular, nucleic acid-templated chemistries are used to produce reaction intermediates attached to an oligonucleotide that can be used to identify the reaction intermediates and/or the resulting...

20060223109 - G protein-coupled receptors: The invention provides isolated nucleic acid and amino acid sequences of novel receptors, antibodies to such receptors, methods of detecting such nucleic acids and receptors, and methods of screening for modulators of the receptors....

20060223093 - Genetic polymorphisms associated with coronary heart disease, methods of detection and uses thereof: The present invention is based on the discovery of genetic polymorphisms that are associated with coronary heart disease and in particular stenosis and MI and response to drug treatment. In particular, the present invention relates to nucleic acid molecules containing the polymorphisms, variant proteins encoded by such nucleic acid molecules,...

20060223088 - Human secreted proteins: The present invention relates to human secreted polypeptides, and isolated nucleic acid molecules encoding said polypeptides, useful for diagnosing and treating immune disorders and diseases. Antibodies that bind these polypeptides are also encompassed by the present invention. Also encompassed by the invention are vectors, host cells, and recombinant and synthetic...

20060223081 - Ladder assembly and system for generating diversity: The present invention provides novel methods of generating a nucleic acid molecule. In certain embodiments, a double stranded nucleic acid chunk is generated from a ladder complex comprising partially complementary oligonucleotides, which chunk is combined with a nucleic acid acceptor molecule. In certain embodiments, the assembled chunk/nucleic acid acceptor molecule...

20060223082 - Map: The invention provides map polypeptides and polynucleotides encoding map polypeptides and methods for producing such polypeptides by recombinant techniques. Also provided are methods for utilizing map polypeptides to screen for antibacterial compounds....

20060223105 - Mass spectrometric methods for detecting mutations in a target nucleic acid: Fast and highly accurate mass spectrometry-based processes for detecting particular nucleic acid molecules and mutations in the molecules are provided....

20060223078 - Method and device for detecting targets with a tape having probes capable of binding to the targets: A tape for detecting bio-analytes is provided. The tape includes a flexible, elongate band defining identifiable sections disposed lengthwise thereon. The tape also includes biological or biochemical probes. At least one probe is attached to a section. Probes in different sections are capable of binding specifically to different biological or...

20060223060 - Method for detecting analytes: The present invention relates to a method and devices for detecting analytes comprising the following stages: incubation of a sample with macromolecules, to each of which at least 2 molecules of the analyte to be detected in the sample are coupled; subsequent incubation of the sample with a solid carrier,...

20060223083 - Method for detecting nucleic acid sequences: A method for detecting nucleic acid sequences in two or more collections of nucleic acid molecules, the method comprising: (a) providing an array of modified polynucleotides bound to a solid surface, each said modified polynucleotide comprising a determinable nucleic acid; (b) contacting the array of modified polynucleotides with: (i) a...

20060223087 - Method for nucleic acid analysis: A simple and highly accurate method for detecting the presence or absence of gene mutation and methylated cytosine in CpG dinucleotide that are contained in a target sequence derived from an analysis sample is provided. Features of the method for nucleic acid analysis include cleaving one or more noncomplementary sites...

20060223063 - Method of diagnosis of type 2 diabetes and early onset thereof: The invention pertains to the field of human genetics and relates to new methods of diagnosis and therapy of type 2 diabetes (T2D), early onset type 2 diabetes and Maturity-Onset Diabetes of the Young (MODY) in a human subject, based on the identification of the A185G variation located at exon...

20060223100 - Method of selectively removing protein from biological samples using chemicals: where at least two of R1, R2, and R3 substituents are substituted or unsubstituted C1-C6 alkyl groups and the other substituent is a hydrogen atom, and a is an integer of 1 to 6; treating the resultant mixture with a hydrophobic surface material in order to obtain a protein-free mixture;...

20060223084 - Methods and compositions for detecting bacillus anthracis: The present invention relates to methods, compositions, and kits for detecting the presence of B. anthracis and binding partners to B. anthracis. The invention also relates to polynucleotide sequences that are specific for the B. anthracis genome and proteins encoded by those sequences....

20060223104 - Methods and compositions for modulating necdin function: Described herein are methods for identifying and using inhibitors of Necdin function to promote brown adipose tissue (BAT) differentiation, thereby treating obesity....

20060223094 - Methods and compositions for producing labeled probe nucleic acids for use in array based comparative genomic hybridization applications: Methods and compositions for producing labeled probe nucleic acids from genomic nucleic acid template are provided. In the subject methods, a conserved coding consensus region primer is employed to enzymatically generate a select set of labeled probe nucleic acids corresponding to coding regions of genes from a genomic template via...

20060223108 - Methods and kits for diagnosing and monitoring hematopoietic cancers: Methods and kits for distinguishing between heparanase expressing and heparanase non-expressing hematopoietic cells, particularly useful in distinguishing between different types of differentiated and/or undifferentiated lymphomas and leukemias. The methods and kits are designed to detect heparanase expression, or absence thereof, at the gene, protein and/or protein activity levels....

20060223059 - Methods for detecting endocrine cancer: Kallikrein 12, kallikrein 14, and kallikrein 15 proteins, and nucleic acids encoding the proteins have particular application in the detection of endocrine cancer, in particular ovarian cancer. Kallikrein 12, kallikrein 14, and kallikrein 15 proteins and nucleic acid molecules encoding the proteins constitute new biomarkers for diagnosis and monitoring (i.e....

20060223066 - Methods for normalizing and for identifying small nucleic acids: The present teachings are generally directed to methods for normalizing at least one species of small nucleic acid that is present in a population of small nucleic acid species, wherein the relative concentration of at least one small nucleic acid species is substantially greater than the relative concentration of at...

20060223064 - Methods of nucleic acid analysis: The present invention relates to a method of characterising a target base in a sample nucleic acid, which method comprises: (a) contacting the sample nucleic acid with an oligonucleotide primer under conditions which allow hybridisation of the oligonucleotide to the sample nucleic acid, said oligonucleotide primer being labelled with a...

20060223072 - Methods of using a dnase i-like enzyme: Methods for expanding conditions of use of a DNase I-like enzyme are disclosed as are compositions and kits comprising a DNAse I-like enzyme. Methods and kits for isolating RNA are also disclosed....

20060223073 - Methods of using a dnase i-like enzyme: Methods for expanding conditions of use of a DNase I-like enzyme are disclosed as are compositions and kits comprising a DNAse I-like enzyme. Compositions comprising storage-stable forms of a DNase I-like enzyme and methods for producing such compositions are also disclosed....

20060223070 - Methods, compositions, and kits for detecting nucleic acids in a single vessel: The invention encompasses processes, compositions, and kits for isolating and detecting nucleic acids from samples using a metal oxide coated onto a vessel. The nucleic acids can be processed and detected within this vessel....

20060223071 - Methods, compositions, and kits for detecting nucleic acids in a single vessel: The invention encompasses processes, compositions, and kits for isolating and detecting nucleic acids from samples using a metal oxide coated onto a vessel. The nucleic acids can be processed and detected within this vessel....

20060223091 - Microarray and methods of using same: A DNA microarray device having probes immobilized to the microarray surface, in which targets to be detected using the microarray are exposed to the probes to hybridize therewith. There are provided on the microarray a quantity of a group of perfect match probes for a target of interest such that...

20060223067 - Mutant dna polymerases and methods of use: The present invention provides mutant DNA polymerases, polynucleotides encoding the polymerases, cassettes and vectors including such polynucleotides, and cells containing the polymerases, polynucleotides, cassettes, and/or vectors of the invention. The present invention also provides methods for synthesizing polynucleotides and kits including a DNA polymerase of the invention....

20060223085 - Nucleic acid detection method: This invention provides a novel method for amplifying and detecting a target gene rapidly with high sensitivity under isothermal conditions. In such method, a sequence to be amplified can be freely designed regardless of the template sequence, an amplified product can be amplified and detected within a short period of...

20060223102 - Polynucleotides and methods for making plants resistant to fungal pathogens: This invention relates to polynucleotide sequences encoding a gene that can confer resistance to the plant pathogen Colletotrichum, which causes anthracnose stalk rot, leaf blight and top dieback in corn and other cereals. It further relates to plants and seeds of plants carrying chimeric genes comprising said polynucleotide sequences, which...

20060223090 - Polynucleotides encoding human secreted proteins: The present invention relates to human secreted polypeptides, and isolated nucleic acid molecules encoding said polypeptides, useful for diagnosing and treating allergic and asthmatic disorders. Antibodies that bind these polypeptides are also encompassed by the present invention. Also encompassed by the invention are vectors, host cells, and recombinant and synthetic...

20060223103 - Procedure and device for determining the concentrations of at least two ligands: In a procedure for determining the concentrations of ligands contained in a sample wherein a first ligand is bond-specific to a first receptor and a second ligand is bond-specific to a second receptor, the first receptor is immobilized on a first test site and the second receptor is immobilized on...

20060223069 - Products and methods for reducing dye artifacts: The present invention provides products and methods of reducing dye artifacts from chain extension reactions....

20060223062 - Rapid direct sequence analysis of multi-exon genes: Disclosed is a Single Condition Amplification/Internal Primer (SCAIP) sequencing method which allows for the rapid, accurate, and economical analysis of any large multi-exon gene. The method can be used to detect genomic mutations in any large multi-exon gene including the dystrophin gene. In some forms, the method can rely on...

20060223089 - Reverse two-hybrid systems: Disclosed are methods for identifying molecular interactions (e.g., protein/protein, protein/DNA, protein/RNA, or RNA/RNA interactions). All of the methods within the invention employ counterselection and at least two hybrid molecules. Molecules which interact reconstitute a transcription factor and direct expression of a reporter gene, the expression of which is then assayed....

20060223068 - Sorting of carbon nanotubes through selective dna delamination of dna/carbon nanotube hybrid structures: A method is described that involves creating bundles of semi-conducting CNTs by passing electromagnetic radiation through a solution containing individual DNA/metallic CNT hybrid structures and individual DNA/semi-conducting CNT hybrid structures. The method also involves segregating the bundles of semi-conducting CNTs from the DNA/metallic CNT hybrid structures. Another method is described...

20060223074 - Spotting compositions and methods of use thereof: Described herein are spotting solutions and methods of use thereof....

20060223095 - System, method and computer program for non-binary sequence comparison: A system and method for performing non-binary comparison of biological sequences includes a new measure ω0, which is a non-binary counting measure that is used in a stand alone module called VaSSA-1. This measure obtains substantially more information about sequences and comparisons between them than is gathered by conventional bioinformatics...

20060223099 - Tex14 compositions and modulators thereof to alter intercellular bridge development and function: The present invention is related to TEX14 and germ cell intercellular bridges and compositions thereof that can be used to inhibit and/or decrease the activity and/or expression of TEX14 and/or disrupt the formation, function, and/or integrity of the intercellular bridges, including localization of key components to the intercellular bridge, for...

20060223075 - Unique sequence hybridization probes (usp): The present invention provides unique sequence probes, methods for generating them, and methods for using unique sequence probes in hybridization assays....

20060223111 - Biosensor system that enables spectral multiplexing: A biosensor includes at least two sensors having different resonant frequencies. The different resonant frequencies of the sensors can be achieved by fabricating the sensors with different spatial profiles of regions of high and low dielectric constant. Because the sensors have different resonant frequencies, they can be monitored in parallel...

20060223110 - Compositions and methods involving nuclear hormone receptor site ii: A binding site in nuclear hormone receptors is described and its structural coordinates are provided. The invention provides machine-readable data storage media comprising structure coordinates of Site II and computer systems comprising the machine-readable data storage media. The invention provides methods used in the design and identification of ligands of...

20060223115 - Heterotrimeric g-protein: Receptor mediated activation of heterotrimeric G-proteins is visualized in living cells by monitoring fluorescence resonance energy transfer (FRET) between subunits of a G protein fused to cyan and yellow fluorescent proteins. The G-protein heterotrimer rapidly dissociates and reassociates upon addition and removal of cognate ligand. Energy transfer pairs of G-proteins...

20060223120 - High-throughput method for optimum solubility screening for homogeneity and crystallization of proteins: Herein is described and optimum solubility screen in which a panel of buffers and many additives are provided in order to obtain the most homogeneous and monodisperse protein condition for protein crystallization. The present methods are useful for proteins that aggregate and cannot be concentrated prior to setting up crystallization...

20060223113 - Immobilization of binding agents: A method of preparing an array of discrete localized binding agent-supporting regions on a solid support surface as well as an array prepared by the method are disclosed. The method comprises: providing a solid support bearing functional groups at a plurality of predefined discrete regions and being in a first...

20060223119 - Methods for monitoring and guiding therapeutic suppression of parathyroid hormone in renal patients having secondary hyperparathyroidism: The present invention relates to kits for monitoring and guiding therapeutic suppression of parathyroid hormone in renal patients having secondary hyperparathyroidism. The kit provides means to measure the level of a PTH agonist and a PTH antagonist in a patient, and it further provides means for administering a PTH suppressing...

20060223116 - Modulation of th2 lineage commitment by t-bet: The instant invention is based, at least in part, on the identification of a mechanism by which T-bet directly modulates Th2 cytokine production. The present invention pertains to methods of identifying agents that modulate the Tec kinase-mediated interaction of T-bet with GATA-3, as well as methods of use therefore....

20060223112 - Novel receptor: The present invention relates to the novel GABAB receptor subtypes GABAB-R1c and GABAB-R2 as well as to a novel, functional GABAB receptor which comprises a heterodimer of GABAB-R1 and GABAB-R2 receptor subunits. The present invention also relates to variants of the receptors, nucleotide sequences encoding the receptors and variants thereof...

20060223117 - Novel splice variants of human epithelial sodium channel genes expressed in human taste tissue and uses thereof: Nucleic acid sequences encoding novel splice variants that encode subunits of an ENaC expressed in human taste tissue are provided. These splice variants when expressed in association with other ENaC subunits, i.e., α, β and γ subunits or α, β and Δ subunits may be used to produce amiloride-insensitive ENACs....

20060223114 - Protein scaffolds and uses thereof: The present invention provides thrombospondin, thyroglobulin and trfoil/PD monomer domains and multimers comprising the monomer domains are provided. Methods, compositions, libraries and cells that express one or more library member, along with kits and integrated systems, are also included in the present invention....

20060223118 - Screening of chemical compounds purified from biological sources: A method of producing a chemical compound library comprises extracting at least one extract from at least one species of plant; processing at least one of the extract(s) to remove at least one type of chemical interference to produce a processed extract; chromatographically separating the processed extract into a plurality...

20060223121 - Use of a polypeptide for detecting, preventing or treating a pathological condition associated with a degenerative, neurological or autoimmune disease: The invention concerns the use of at least one polypeptide comprising a protein fragment to obtain a diagnostic, prognostic, prophylactic or therapeutic composition for detecting, preventing or treating a pathological condition associated with a degenerative and/or neurological and/or autoimmune disease, said protein being selected among the proteins whereof the peptide...

20060223124 - Amphiphilic mediated sample preparation for micro-flow cytometry: A flow cytometer includes a flow cell for detecting the sample, an oil phase in the flow cell, a water phase in the flow cell, an oil-water interface between the oil phase and the water phase, a detector for detecting the sample at the oil-water interface, and a hydrophobic unit...

20060223122 - Classifying and predicting glomerulosclerosis using a proteomics approach: The present invention provides for a proteomic approach to classifying glomerular tissue as normal, non-sclerotic or sclerotic. In particular, the proteomic approach may employ laser capture microdissection followed by MALDI-TOF. A particular target of interest that is highly relevant in distinguishing such tissues is thymosin β4....

20060223123 - Rhesus monkey nur77: The Macaca mulatta (rhesus monkey) Nur77 (rhNur77) nuclear receptor and the nucleic acid encoding the rhNur77 nuclear receptor are described. Further described are methods for identifying analytes that modulate expression or activity of the rhNur77 nuclear receptor. Analytes that affect Nur77 expression or activity may be used to treat or...

20060223129 - Compounds for immunodiagnosis of prostate cancer and methods for their use: Compounds and methods for diagnosing prostate cancer are provided. The inventive compounds include polypeptides containing at least a portion of a prostate tumor protein. The inventive polypeptides may be used to generate antibodies useful for the diagnosis and monitoring of prostate cancer. Nucleic acid sequences for preparing probes, primers, and...

20060223128 - Method for detecting multi-drug resistance: The present invention stems from the realization that the cell surface content of sialic acids is associated with drug susceptibility and resistance in neoplastic and damaged cells. A general decrease in the amounts of the α2-6 linked sialic acid has been confirmed in resistant phenotype compare to its corresponding sensitive...

20060223127 - Serum biomarkers in lung cancer: Certain biomarkers and biomarker combinations are useful in a qualifying lung cancer status in a subject. A diagnostic methodology employing these biomarkers and combinations can detect whether a subject has lung cancer....

20060223126 - Magnetic particles having porous surfaces, method of producing the same, carrier for biochemical use, and biotin-bonding particles: Magnetic particles having porous surfaces are obtained by forming a polymer part (B) soluble in an organic solvent (S) and a polymer part (C) insoluble in the organic solvent (S) on surfaces of magnetic matrix particles (A) and causing the polymer part (B) to come into contact with the organic...

20060223125 - Methods and kits for staining cell membranes: Methods and kits are provided for staining cell membranes using a biotinylated phospholipid in conjunction with streptavidin-coated quantum dots....

20060223130 - Detection of post-translationally modified analytes: The invention provides methods of analyzing a sample. In general, the methods involve: a) contacting a sample with an array of capture agents; and b) reading the array to detect the presence of a post-translational modification indicator and an analyte-detection moiety. The analytes of the sample may be contacted with...

20060223134 - Doxorubicin immunoassay: Novel conjugates of doxorubicin and novel doxorubicin immunogens derived from the 13 and 14 positions of doxorubicin and antibodies generated by these doxorubicin linked immunogens all of which are useful in immunoassays for the quantification and monitoring of doxorubicin in biological fluids....

20060223133 - Monoclonal antibody against connective tissue growth factor and medicinal uses thereof: A human monoclonal antibody useful for the treatment of various diseases caused by human connective tissue growth factor (CTGF) and preventing the onset of the above diseases; medicinal uses thereof; and various monoclonal antibodies having various characteristics against various mammalian CTGFs useful for detecting and assaying CTGFs present in body...

20060223132 - Phenethanolamine-derived haptens, immunogens, antibodies and conjugates: h

20060223131 - Protein arrays and methods of use thereof: The present invention provides human protein arrays that include at least 1000 human proteins. In another embodiment, the present invention provides a method for identifying a substrate of an enzyme, comprising contacting the enzyme with a positionally addressable array comprising at least 100 proteins immobilized on functionalized glass surface, and...

20060223135 - High throughput reactive oxygen species-based cytochrome p450 inhibition assay: A method of screening a candidate compound for an ability to inhibit a cytochrome P450. The method includes the steps of reacting the candidate compound, an indicator compound precursor, a cytochrome P450 substrate, and the cytochrome P450, the cytochrome P450 characterized as having a side reaction associated with metabolic activity...

20060223136 - Scytalone dehydrogenase gene showing tolerance to agricultural pesticide: The present invention provides a gene that can be used extensively in studies relating to resistant rice blast fungi. The gene codes for either one of the following proteins (a) or (b): (a) a protein consisting of the amino acid sequence shown in SEQ ID NO:2; or (b) a protein...

20060223137 - Reagent for partially lysing a cell membrane of a red blood cell, a reagent for detecting malaria infected red blood cells, and a sample analyzing method for detecting malaria infected red blood cells: A reagent for partially lysing a cell membrane of a red blood cell infected with a malaria parasite, comprising: a first surfactant which has predetermined lysing ability for the red blood cell membrane; and a second surfactant which has weaker lysing ability than that of the first surfactant; wherein the...

20060223138 - Method and apparatus for counting megakaryocytes: A method for counting megakaryocytes in a specimen is described. In the method, first, erythrocytes in the specimen are lysed and nucleic acid in the megakaryocytes is stained with a fluorescent dye, and thereby, a measurement sample is prepared. Next, the cells in the measurement sample are irradiated with excited...

20060223139 - Use of fluorinated fluids as storage liquid for preserved biological specimens: A method of preserving a biological tissue specimen comprising at least one of fixing or dehydrating a specimen, and substantially completely immersing the specimen in a preserving fluid comprising one or more fluorinated hydrocarbons. Also, a biological tissue specimen immersed in a preserving fluid comprising one or more fluorinated hydrocarbon...

20060223140 - Transglycosylation method and protein having transglycosylation activity: The present invention provides a transglycosylation method and a protein catalyzing a transglycosylation reaction. A reaction of transferring a galactosylβ1→3N-acetylgalactosaminyl group from a sugar donor containing a sugar chain having a galactosylβ1→3N-acetylgalactosaminyl group bound thereto at the α-position, to a sugar chain acceptor, is carried out in the presence of...

20060223142 - Extended cdnas for secreted proteins: The sequences of extended cDNAs encoding secreted proteins are disclosed. The extended cDNAs can be used to express secreted proteins or portions thereof or to obtain antibodies capable of specifically binding to the secreted proteins. The extended cDNAs may also be used in diagnostic, forensic, gene therapy, and chromosome mapping...

20060223143 - Methods and compositions for polypeptide engineering: Methods are provided for the evolution of proteins of industrial and pharmaceutical interest, including methods for effecting recombination and selection. Compositions produced by these methods are also disclosed....

20060223141 - Transcription amplification system for molecular imaging: The present invention provides a transcription amplification system, comprising an effector nucleic acid molecule and a reporter molecule, which work together to produce a heterologous gene product in a cell-type, specific manner. The present invention also relates to methods for producing, detecting, imaging, and monitoring the heterologous gene product in...

20060223144 - Method for mass production of human pollicle stimulating hormone: The present invention relates to a method for mass-production of human follicle stimulating hormone, more precisely, to an expression vector containing human follicle stimulating hormone gene, a transformant transfected with the expression vector and a method for mass-production of human follicle stimulating hormone by using the same. Human follicle stimulating...

20060223145 - Method for producing recombinant human interferon alpha 2b polypeptide in pichia pastoris: This invention relates to a process for expression of recombinant Interferon-alfa2b polypeptide in yeast cells and its method of purification and formulation....

20060223146 - Midecamycin hyper producing strain: A midecamycin hyper producing strain having improved productivity of midecamycin which is a member of macrolide antibiotics, and a method for producing midecamycins using the strain are provided. The midecamycin producing actinomycetes comprises a midecamycin biosynthesis gene or a homologue thereof, wherein at least one module in a polyketide synthase...

20060223147 - Process for producing glycoprotein composition: The present invention relates to a cell into which an RNA capable of suppressing the function of an enzyme catalyzing a reaction which converts GDP-mannose into GDP-4-keto,6-deoxy-GDP-mannose is introduced; a process for producing a glycoprotein using the cell; a cell into which an RNA capable of suppressing the function of...

20060223148 - Process for sequence saturation mutagenesis (sesam): A process for the mutagenesis of a double-stranded polynucleotide sequence (master sequence) of n base-pairs having a (+)-strand and a complementary (−)-strand comprising the steps (i) creation of a collection of single-stranded fragments of the (+)-strand of the master sequence wherein all members of the collection have the same 5′-terminus...

20060223149 - Methods for producing sulphurous fine chemicals: The invention relates to methods for the fermentative production of sulfur-containing fine chemicals, in particular L-methionine, by using bacteria which express a nucleotide sequence coding for a methionine synthase (metH) gene....

20060223150 - Process for the isolation and purification of mevinolin: In a process for preparing mevinolin by fermentation of a biomass in a fermentation liquor, which includes dissolving mevinolin from the biomass into the fermentation liquor, and separating the biomass from the fermentation liquor to obtain a separated fermentation liquor, separating the mevinolin from the separated fermentation liquor, and recovering...

20060223151 - Production of polyketides: Recombinant host cells of the suborder Cystobacterineae containing recombinant expression vectors that encode heterologous PKS genes can produce polyketides synthesized by the PKS enzymes encoded on those vectors at high levels....

20060223152 - Optically active 2-allylcarboxylic acid derivative and process for producing the same: The present invention provides a process for producing an optically active 2-allylcarboxylic acid derivative, which is useful as a pharmaceutical intermediate, from readily available and inexpensive starting materials by the process which can be practiced on a commercial scale in a simple and easy manner, and certain 2-allylcarboxamide derivatives, which...

20060223153 - Generation of materials with enhanced hydrogen content from anaerobic microbial consortia: A microbial consortia for biogenically increasing the hydrogen content of a carbonaceous source material, where the consortia includes a first microbial consortium to metabolize the carbonaceous source material into one or more first intermediate hydrocarbons, a second microbial consortium, which includes one or more species of Pseudomonas microorganisms, to convert...

20060223154 - Method and bioreactor for producing synfuel from carbonaceous material: A method of producing fuel from biodegradable carbonaceous material using a stacked particle bioreactor is provided. A stacked particle bioreactor is formed from particles including biodegradable carbonaceous material. The biodegradable carbonaceous material in the stacked particle bioreactor is aerobically and/or anaerobically bioconverted into one or more synfuels, which are collected...

20060223155 - Enhancement of in vitro culture or vaccine production in bioreactors using electromagnetic energy: Disclosed are apparatus and methods for enhancing or improving cell cultures, including cell cultures for the production of monoclonal antibodies, using electromagnetic energy treatment, primarily using electromagnetic radiation in the near infrared to visible region of the spectrum. The delivery of electromagnetic energy to a culture, in accordance with preferred...

20060223156 - Controlled dissolution crosslinked protein in crystals: The present invention relates to crosslinked protein crystals characterized by the ability to change from insoluble and stable form to soluble and active form upon a change in the environment of said crystals, said change being selected from the group consisting of change in temperature, change in pH, change in...

20060223157 - Polypeptides having nucleic acid binding activity: Polypeptides having nucleic acid binding activity are provided. Methods of stabilizing a nucleic acid duplex are provided. Methods of promoting the annealing of complementary nucleic acid strands are provided. Methods of increasing the processivity of a DNA polymerase are provided. Methods of enhancing the activity of a nucleic acid modification...

20060223158 - Regulation of neuronal function through metabotropic glutamate receptor signaling pathways: The present invention provides methods and compositions for modulating the activities of metabotropic glutamate receptor intracellular signaling molecules. The present invention provides methods and compositions for modulating the activities of casein kinase I and/or cyclin-dependent kinase 5 in cells or tissues. The present invention provides methods of modulating the function...

20060223159 - Generation of materials with enhanced hydrogen content from microbial consortia including thermotoga: A microbial consortia for biogenically increasing the hydrogen content of a carbonaceous source material, where the consortia includes a first microbial consortium to metabolize the carbonaceous source material into one or more first intermediate hydrocarbons, a second microbial consortium, which includes one or more species of Thermotoga microorganisms, to convert...

20060223160 - Systems and methods for the isolation and identification of microorganisms from hydrocarbon deposits: The present invention provides systems and methods to isolate microorganisms and optionally their genetic material. The isolation of microorganisms is conducted under anaerobic or other similar conditions to facilitate the preparation of microorganisms that are anaerobes, whether obligate or facultative, as well as microaerophiles. In an alternative format, the isolation...

20060223161 - Novel enterococcus and streptococcus strains and bacteriocins: Novel Enterococcus and Streptococcus bacteriocins produced by novel Enterococcus and Streptococcus strains are used for at least reducing the levels of colonization by at least one target bacteria in animals, especially poultry....

20060223162 - Method for accelerated dechlorination of matter: Accelerated dechlorination of soil and water contaminated with chlorinated solvents is achieved by stimulating anaerobic microorganisms and thus increasing the rate of biological mineralization of the solvents. This is accomplished by a treatment process consisting of colloidal suspension of metal powder, an organic hydrogen donor, chemical oxygen scavengers in solution...

20060223163 - Apparatus for capturing cell: A bottom wall of a Petri dish used for capturing a cell is provided with a first through hole and a second through hole. A grove that connects the first through hole and the second through hole is formed on an outer surface of bottom wall. The first through hole,...

20060223164 - Systems and methods for rapidly changing the solution environment around sensors: The invention provides microfluidic systems for altering the solution environment around a nanoscopic or microscopic object, such as a sensor, and methods for using the same. The invention can be applied in any sensor technology in which the sensing element needs to be exposed rapidly, sequentially, and controllably, to a...

20060223165 - Device for counting cells and method for manufacturing the same: Disclosed are a device for counting cells and a method for manufacturing the device. The device comprises a transparent upper substrate and a transparent lower substrate. Fine lattice patterns for counting cells are formed on the lower substrate. A space for filling the sample, including cells, is provided between the...

20060223166 - Mesoscale detection structures: Disclosed are devices for detecting the presence of a preselected analyte in a fluid sample. The devices comprise a substrate microfabricated to define a sample inlet port, and a mesoscale flow system that includes a sample flow channel extending from the inlet port. The mesoscale flow system further includes an...

20060223167 - Biochip support comprising thin layers of sol-gel material and production method thereof: The invention relates to a biochip support comprising a substrate supporting at least one porous layer of material on a first face, the said layer being designed to fix biological molecules onto the said layer and in the volume of this layer, the said support being characterized in that the...

20060223170 - Biomolecule detecting apparatus and biomolecule detecting method employing the same: A biomolecule detecting element capable of easily immobilizing a detecting probe and being used in a simple manner without requiring a dark box and the like. A biomolecule detecting probe is immobilized on the surface of a conductive electrode of an insulated gate field effect transistor, the conductive electrode being...

20060223171 - High sensitivity mechanical resonant sensor: A system and method for detecting mass based on a frequency differential of a resonating micromachined structure, such as a cantilever beam. A high aspect ratio cantilever beam is coated with an immobilized binding partner that couples to a predetermined cell or molecule. A first resonant frequency is determined for...

20060223168 - Integrated cmos-compatible biochip: A device for analyzing a fluid sample is provided. The device includes a substrate, a trench formed in said substrate, and a processor. The trench includes a channel, a sample chamber, and a reagent chamber, each in fluid communication with each another. The sample chamber is configured to receive the...

20060223169 - Multiplex fluorescence detection device having removable optical modules: Techniques are described for the detection of multiple target species in real-time PCR (polymerase chain reaction). For example, a system comprises a data acquisition device and a detection device coupled to the data acquisition device. The detection device includes a rotating disk having a plurality of process chambers having a...

20060223172 - Multiplex fluorescence detection device having fiber bundle coupling multiple optical modules to a common detector: Techniques are described for the detection of multiple target species in real-time PCR (polymerase chain reaction). For example, a system is described that includes a data acquisition device and a detection device coupled to the data acquisition device. The detection device includes a rotating disk having a plurality of process...

20060223173 - Anaerobic digester for the production of methane gas from manure: An anaerobic digester to produce methane gas from animal manure is proposed to be more efficient and convenient for farmers and ranchers. The proposed invention utilizes already familiar containers such as propane tanks for the digester to produce the methane gas; and a tractor PTO (i.e.) power turn out to...

20060223174 - Insert device for culturing cells: The present invention involves a device for culturing cells on removable membranes/sponges for the purpose of transplantation. The device includes a base plate with snaps and a ring, which fits into the base plate. The membrane/sponge of choice for culturing cells is placed on the base plate and fixed by...

20060223175 - Bioreactor for cultivating tissue cells: A bioreactor for cultivating tissue cells comprises a vessel containing a gas phase and a liquid phase therein, at least a substrate on which the tissue cells are attached, and a movable shaft to which the substrate is fixed. The movable shaft carries the substrate into and out of the...

20060223178 - Devices and methods for magnetic enrichment of cells and other particles: The invention features devices and methods for the enrichment of cells and other desired analytes by employing a magnetic field, alone or in conjunction with size-based separation. The devices and methods may be advantageously employed to enrich for rare cells, e.g., fetal cells or epithelial cells, present in a sample,...

20060223176 - Integrated system for on-site cell acquisition, processing, and delivery: Numerous embodiments of a system and method for treating cardiac tissue are described. In one embodiment, bone marrow cells are extracted from a patient. The cells are then processed to isolate mononuclear cells, which can then be delivered back near the cardiac tissue of the patient....

20060223177 - Postpartum cells derived from umbilical cord tissue, and methods of making and using the same: Cells derived from human umbilical cords are disclosed along with methods for their therapeutic use. Isolation techniques, culture methods and detailed characterization of the cells with respect to their cell surface markers, gene expression, and their secretion of trophic factors are described....

20060223179 - Differentiation of pluripotent embryonic stem cells: The invention relates to a method to induce primate embryonic stem cells to differentiate into a relatively homogenous population of mesoendoderm cells by treatment with caspase-like inhibitors. Also described is a population of mesoendoderm cells obtained therefrom. The embryonic stem cell derived mesoendoderm cells have the general morphological and cell...

20060223180 - Methods to mobilize progenitor/stem cells: wherein each R is independently H or straight, branched or cyclic alkyl (1-6C), n is 1 or 2, and X is an aromatic ring, including heteroaromatic rings, or is a mercaptan; “linker” represents a bond, alkylene (1-6C) or may comprise aryl, fused aryl, oxygen atoms contained in an alkylene chain,...

20060223181 - Method for separating hepatic progenitor cell: The present invention relates to a method for identifying a pluripotent hepatic progenitor cell, a method for separating the pluripotent hepatic progenitor cell and a method for producing the pluripotent hepatic progenitor cell....

20060223182 - Device for producing autologous vegf: A method of adding an effective amount of reactive oxygen species (ROS) to a mixture of macrophages to induce VEGF production by the macrophages. The induced macrophages are then added to a graft material....

20060223183 - Gamma irradiation of frozen feeder cells: Gamma irradiation of frozen feeder cells improves their function, uniformity and extends their usable lifespan, thereby allowing a more robust and flexible stem cell culture system. Feeder cells that are irradiated while frozen do not suffer the reduced functional lifespan of feeder cells that are irradiated and then frozen and...

20060223184 - Supports useful in incorporating biomolecules into cells and methods of using thereof: Described herein are supports useful in incorporating biomolecules into cells and methods of making and using thereof....

20060223185 - Electrosonic cell manipulation device and method of use thereof: In method of injecting a substance into a living cell having a cell membrane, the substance, the cell and a liquid are placed into a tapering passage. Energy is applied to the cell, thereby inducing poration. To sort cells, a cellular suspension is placed in a tapering passage, including a...

20060223186 - Double transposition methods for manipulating nucleic acids: Methods are provided for manipulating nucleic acid to produce gene fusions, to delete or clone a portion of a chromosome, or to insert a sequence into a chromosome. The methods employ sequential transposition processes using two or more pairs of inverted repeat transposase-interacting sequences on a transposable polynucleotide wherein each...