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Chemistry: molecular biology and microbiology September class, title,number 09/06

20060210959 - System and method to simulate hemodynamics: A system for hemodynamic simulation comprises a vessel having properties of a blood vessel, a reservoir containing a quantity of fluid, tubing connecting the vessel and reservoir, and at least one pump for circulating the fluid within the system. Fluid can be tissue culture medium or blood analog fluid, and...

20060210960 - Method for processing and preserving collagen-based tissues for transplantation: A method for processing and preserving an acellular collagen-based tissue matrix for transplantation is disclosed. The method includes the steps of processing biological tissues with a stabilizing solution to reduce procurement damage, treatment with a processing solution to remove cells, treatment with a cryoprotectant solution followed by freezing, drying, storage...

20060210962 - Cell observation device and cell observation method: The cell observation device and method aims to easily study the correlation between initial states of cells and their reactions without using a synchronous culture method. For this purpose, the cell observation device includes an image acquisition unit acquiring an image of a specimen including a plurality of cells; a...

20060210963 - Induction of a beta cell differentiation in human cells: The present invention provides methods for inducing insulin gene expression in cultured pancreas cells, the method comprising contacting a culture of endocrine pancreas cells expressing a PDX-1 gene and a NeuroD/BETA2 gene with a GLP-1 receptor agonist, wherein the cells have been cultured under conditions such that the cells are...

20060210961 - Method and system for analysing a liquid sample: e

20060210964 - Prevention and treatment of amyloid-associated disorders: The present invention provides assays to identify compounds that affect microglial cell activation, and specifically assays to identify compounds that affect secretion of cytokines from these microglial cells by modulating PGE2-mediated activity. The assays of the invention include assays for testing microglial cell activation by contacting microglia with compounds that...

20060210965 - Efficient generation of adenovirus-based libraries by positive selection of adenoviral recombinants through ectopic expression of the adenovirus protease: Disclosed is a new system for generating recombinant adenovirus vectors and adenovirus-based expression libraries, by positive selection of recombinants deleted for the endogenous protease gene, which gene is expressibly cloned into another region of the adenoviral genome. In a preferred embodiment, the invention allows positive selection of E1-deleted, protease-deleted recombinant...

20060210969 - Infectious, chimeric hepatitis c virus, methods of producing the same and methods of use thereof: The present invention provides infectious recombinant Hepatitis C Viruses (HCV), and vectors, cells and animals comprising the same. The present invention provides methods of producing infectious recombinant HCV, and their use in identifying anti-HCV therapeutic agents, as well as sequences of HCV associated with HCV pathogenesis....

20060210966 - Kits and methods for detecting bovine ephemeral fever virus: The present invention relates to a kit and method for detecting BEFV of suspected patient. The present invention also relates to primers and probe used to detect BEFV....

20060210970 - Methods, compositions and kits for biomarker extraction: The present invention is directed to methods for extracting markers from biological samples, and to systems, devices, kits and reagents for use in such methods. The invention is also to methods, kits, reagents and compositions for measuring a plurality of different organism types in a sample. One of the specific...

20060210967 - Re-sequencing pathogen microarray: The present invention relates to pathogen detection and identification by use of DNA resequencing microarrays. The present invention also provides resequencing microarray chips for differential diagnosis and serotyping of pathogens present in a biological sample. The present invention further provides methods of detecting the presence and identity of pathogens present...

20060210968 - Template reporter bacteriophage platform and multiple bacterial detection assays based thereon: The invention is a method for the development of assays for the simultaneous detection of multiple bacteria. A bacteria of interest is selected. A host bacteria containing plasmid DNA from a T even bacteriophage that infects the bacteria of interest is infected with T4 reporter bacteriophage. After infection, the progeny...

20060210972 - Annotation of genome sequences: A method of identifying one or more proteins in an unannotated DNA sequence is disclosed. The method involves dividing the DNA sequence into a plurality of sequence fragments of substantially the same length (about 300 to 5000 base pairs, most typically 1000 to 1050 base pairs. A six frame translation...

20060211002 - Antibiotic susceptibility and virulence factor detection in pseudomonas aeruginosa: The present invention relates in general to the detection of antibiotic resistance determinants in Pseudomonas aeruginosa (P. aeruginosa). The present invention discloses a micro-array for the detection of antibiotic resistance determinants and mutations in said organism, a method for the detection of said determinants and a kit. This micro-array concept...

20060211008 - Beta-parvin expression for use in diagnostic methods for assessment of breast cancer: The invention is directed to polynucleotides encoding β-parvin, a newly identified tumor suppressor gene the alteration of which is involved in the occurrence of breast cancer in a patient, as well as to corresponding expressed polypeptides. The invention also concerns diagnostic methods using the β-parvin polynucleotides and/or peptides and embodiments...

20060211006 - Bovine castgene snp and meat tenderness: The present invention relates to the identification of a single nucleotide polymorphism (SNP) within the bovine CAST locus encoding the calpastatin protein, wherein the allelic variation of the SNP is a G/C transversion associated with post-mortem muscle tenderness. The invention further relates to oligonucleotides useful in identifying the genotype of...

20060210982 - Cleavable assigned molecules and screening method using the same: A method for screening a nucleic acid library for a nucleic acid encoding a protein that interacts with a target substance, which comprises: the step of producing a library of assigning molecules each comprising a protein and a nucleic acid encoding the protein linked to each other via a linker...

20060210997 - Composition and method for array hybridization: The present disclosure relates to a system, composition, and method for hybridizing a microarray. The composition includes a superwetting agent. The method includes contacting the microarray with an aqueous mixture including the superwetting agent....

20060210974 - Compositions and methods for preventing, treating and diagnosing diabetes: The invention provides methods and compositions for preventing, treating and diagnosing diabetes....

20060211022 - Detection of compounds that affect therapeutic activity: The present invention relates to methods of detecting compounds that affect the activity of a therapeutic substance or composition administered to a subject, and to reagents for use in such methods....

20060210998 - Determination of antibiotic resistance in staphylococcus aureus: The present invention relates to the detection of antibiotic resistance determinants in Staphylococcus aureus. The present invention discloses a micro-array for the detection of antibiotic resistance determinants and mutations in said organism, a method for the detection of said determinants and mutations and a kit....

20060211028 - Dimeric and trimeric nucleic acid dyes, and associated systems and methods: Dimeric and trimeric nucleic acid dyes, and associated systems and methods are provided. Such a dye may form a hairpin-like structure that enables it to stain nucleic acids via a release-on-demand mechanism, for example. Such a dye may have low background fluorescence in the absence of nucleic acids and high...

20060210985 - Dna fragment amplification method, reaction apparatus for amplifying dna fragment and process for producing the same: A reaction apparatus (10) includes a substrate (12) and a plurality of columnar members (14) formed on the substrate (12). Oligonucleotides for immobilization (16) having sequences complementary to sequences of both ends of a starting template DNA (18) is adhered on the surfaces of the substrate (12) and the columnar...

20060210993 - Dna structures on ferroelectrics and semiconductors: An apparatus includes a substrate and a plurality of DNA oligomers in contact with a top surface of the substrate. The substrate is a polar ferroelectric or a polar compound semiconductor....

20060211016 - Electrodes linked via conductive oligomers to nucleic acids: The invention relates to nucleic acids covalently coupled to electrodes via conductive oligomers. More particularly, the invention is directed to the site-selective modification of nucleic acids with electron transfer moieties and electrodes to produce a new class of biomaterials, and to methods of making and using them....

20060211017 - Expression profile of prostate cancer: The present invention relates to compositions and methods for cancer diagnostics, including but not limited to, cancer markers. In particular, the present invention provides gene expression profiles associated with prostate cancers. Genes identified as cancer markers using the methods of the present invention find use in the diagnosis and characterization...

20060210990 - Fluorescence polarisation: There is described a method for detecting alkylated cytosine in double stranded DNA employing one or more enzymes that differentially modify alkylated cytosine and cytosine. At least one region of the DNA is converted to single stranded DNA and the enzyme is reacted with a target region in the single...

20060210973 - Inhibitors of proteins from the rho-gef family: A method for screening peptides with an aptamer library for determining inhibitors of any one of the proteins from the Rho-GEFs family....

20060210991 - Interleukin-8 homologous polypeptides and therapeutic uses thereof: The present invention is directed to novel polypeptides having structural homology to IL-8 and to nucleic acid molecules encoding those polypeptides. Also provided herein are vectors and host cells comprising those nucleic acid sequences, chimeric polypeptide molecules comprising the polypeptides of the present invention fused to heterologous polypeptide sequences, antibodies...

20060211035 - Kits for predicting transplant rejection: The present invention relates to a method for predicting whether or not transplant recipients are likely to reject tissue allografts. It is based, at least in part, on the discovery that based on analysis of three immunologic factors, cardiac transplant recipients could be classified into risk categories for progression to...

20060211010 - Labeled nucleotide phosphate (np) probes: The present invention is directed to a method of sequencing a target nucleic acid molecule having a plurality of bases. In its principle, the temporal order of base additions during the polymerization reaction is measured on a molecule of nucleic acid, i.e. the activity of a nucleic acid polymerizing enzyme...

20060211039 - Ldl receptor signaling pathways: The invention provides methods and compositions for inducing and detecting signal transduction through LDL receptors, including specifically detecting a stress that alters a functional interaction of a low density lipoprotein (LDL) receptor binding polypeptide with an LDL receptor interaction domain, by (a) introducing a predetermined stress into a system which...

20060211007 - Ligand/binding partner bio-labeling systems: The present invention relates to methods and compositions which utilize binding relationships between detector molecules and their ligands for labeling molecules of interest in vivo. A “target” molecule is linked to a detector molecule (such as a protein or a nucleic acid) and the function and/or location of the target...

20060211036 - Metastasis-associated gene profiling for identification of tumor tissue, subtyping, and prediction of prognosis of patients: The present invention provides methods using a gene expression profiling analysis (1) to determine whether a human sample is a tumor using a gene set containing nucleic acid sequences of SEQ ID NOS: 1-7, 8-17 or 1-17; (2) to identify whether a tumor tissue is an adenocarcinoma (using a gene...

20060211014 - Method for detecting bacteria of the genus mycobacterium (acid-fast bacteria) and kit for the same: An object of the present invention is to provide an oligonucleotide for rapidly and conveniently detecting bacteria of the genus Mycobacterium (acid-fast bacteria) or for identifying the bacterial species thereof, and a method and kit for detecting bacteria of the genus Mycobacterium (acid-fast bacteria) using such oligonucleotid. The present invention...

20060210981 - Method for detecting the mutant genes of the major gene cacna1h relating to the childhood absence epilepsy and the cacna1h mutant genes: The present invention relates to a method of detecting the CACNA1H mutant gene of childhood absence epilepsy-the main function gene, the said method is directly sequencing or restriction analysis. The present invention relates to CACNA1H mutant gene. The present invention further relates to the use of the said detection and...

20060210988 - Method for isolating nucleic acid and, for nucleic acid isolation, kit and apparatus: Nucleic acids suitable for PCR amplification are isolated from a sample easily and rapidly by dissolving the sample in a buffer containing surfactant and salt, heating the obtained solution, subjecting the heated solution to gel filtration; and collecting a fraction containing nucleic acids....

20060211013 - Method for sorting antifungal molecules acting on the glucanosyltransferase activity: The invention discloses proteins having β(1-3)glucanosyltransferase type activities. These proteins can be used for detecting the antifungal activity of molecules....

20060210987 - Method for the spatially resolved determination of physcial, chemical and/or biological properties or state variables: Method for the spatially resolved determination of physical, chemical and/or biological properties or state variables and/or the change therein in an examination area of an examination object by determining the change in the spatial distribution and/or the mobility, particularly the mobility in rotation, of magnetic particles in this examination area...

20060210986 - Method of determining state variables and changes in state variables: The present invention relates to a method of determining physical, chemical and/or biological state variables, particularly substance concentrations, temperature, pH and/or physical fields, and/or the change in these state variables in an examination area of an examination object by determining the change in the spatial distribution of magnetic particles in...

20060211023 - Method of diagnosing breast cancer and compositions therefor: A method of diagnosing breast cancer in an individual aged younger than 41 or older than 54 is provided. The method comprises obtaining a nucleic acid from the individual and determining a nucleotide at a polymorphic site of the nucleic acid. Polynucleotides specific to breast cancer, a microarray comprising the...

20060211032 - Method of inactivating ribonucleases at high temperature: Methods for protecting RNA from RNase degradation and for inactivating RNases in solution are disclosed. The invention includes methods for protecting RNA during storage, for performing quantitative PCR reactions, or for preparing cDNA. The method includes using a combination of an RNase inhibitor in a solution containing or devoid of...

20060211005 - Method of measuring pyrophosphate and method of detecting primer extension reaction, and device for performing the same: In the method of measuring PPi of the present invention, a PPi sample having an unknown concentration is allowed to act on mycobacterial H+-pyrophosphatase (H+-PPase) intrinsically included in membrane, thereby measuring the PPi concentration in the sample by analyzing thus resulting H+transport. Furthermore, in the method of detecting an extension...

20060211030 - Methods and compositions for assay readouts on multiple analytical platforms: The invention provides methods and compositions for reading out the results of multiplex assays on various analytical platforms, such as microarrays, bead arrays, electrophoresis devices, and the like. An important feature of the invention includes methods for converting different sets of oligonucleotide tags used for labeling into oligonucleotide tags specific...

20060211004 - Methods and compositions for determining non-specific cytotoxicity of a transfection agent: Methods and compositions for determining cytotoxicity of a transfection agent are provided. In one aspect, a cell is contacted with a transfection agent. Following contact, a cytotoxic marker profile of the cell is evaluated to determine whether the transfection agent has mediated a cytotoxic response in the cell. Also provided...

20060211021 - Methods and compositions for evaluating cell function in sensory neurons: Methods and compositions are provided for determining whether target, e.g., an endogenous or novel (non-endogenous), nucleic acid sequence of a neuronal cell is involved in sensory function. In practicing the subject methods, a neuronal cell selective vector including a modulating domain for a neuronal cell nucleic acid sequence, optionally having...

20060210992 - Methods and compositions for phenotype identification based on nucleic acid methylation: Methods and compositions for identifying an unknown phenotype of a tissue that correlates with changes in the methylation state of the tissue comprising, nucleic acid sample from the tissue with a reagent that modifies unmethylated cytosine to produce uracil, amplifying the nucleic acid target gene region using at least one...

20060210976 - Methods and nucleic acids for the analysis of methylation patterns within the dd3 gene: The disclosed invention provides methods and sequences for the analysis of methylation patterns within the 5′ and promoter regions of the gene DD3....

20060211019 - Methods and probes for detecting esophageal cancer: Probe sets and methods of using probes and probe sets for selectively detecting high grade dysplasia and esophageal adenocarcinoma or low grade dysplasia from biologic samples are described. Methods of the invention include contacting a biological sample obtained from a subject with a set of chromosomal probes to selectively detect...

20060211024 - Methods for analysis of a nucleic acid sample: An assay for analysis of a nucleic acid sample is provided. In one embodiment, the assay includes: a) employing an RNA sample to obtain a DNA probe; b) contacting the DNA probe with a substrate containing a surface-immobilized RNA oligonucleotide to produce a surface-immobilized RNA/DNA duplex; and c) detecting RNAseH-dependent...

20060211026 - Methods for assessing emphysema: Emphysema and COPD are diagnosed, and the efficacy of therapeutic drug candidates for the treatment of emphysema and/or COPD is evaluated, by determining biomarkers selected from the group SpB, desmosine, VEGF, IGFBP2, MMP12, TIMP1, MMP9, Crabp2, Rbp1, Cyp26a1, Tgm2, Timp3, Adam17, Serpina1, Slpi, Col1a1, Eln, TGFβ1, TGFβ-RII, Sftpa1, Csf2, Cxcl1,...

20060211038 - Methods for detecting vasculopathies and tumors: This invention relates generally to the field of prognosis and diagnosis of vasculopathy and certain tumors. In particular, the invention provides a method for prognosing or diagnosing vasculopathy, neuroectodermal tumor, epithelial tumor, myelomonocytic tumor or lymphoid tumor in a mammal. The invention can also be used for monitoring the effectiveness...

20060210996 - Methods for in situ generation of chemical arrays: Methods and devices for producing chemical arrays are provided. Aspects of methods include employing a dedicated wash fluid reaction chamber. In certain embodiments, aspects include contacting a surface with at least a deblocking reagent to produce a deblocked surface, washing the deblocked surface in a dedicated wash fluid reaction chamber,...

20060211020 - Methods for the diagnosis, prognosis and treatment of metabolic syndrome: The present invention provides methods for detecting susceptibility to metabolic syndrome. In particular, the presence of differences in at least one of the following genes; microsomal triglyceride transfer protein (MTP), fatty acid binding protein 2 (FABP2), annexin A5 (ANXA5), pyruvate dehydrogenase (lipoamide) alpha 2 (PDHA2), CDP-diacylglycerol synthase (phosphatidate cytidylyltransferase) 1...

20060210979 - Methods of detecting gene expression in normal and cancerous cells: The present invention provides methods for detecting gene expression in normal and cancerous cells. Specifically, provided are methods utilizing molecular beacons (MB) technology combined with fluorescence imaging techniques for detecting, identifying or quantitating the presence of, or alterations in gene expression of, various tumor markers in a sample of cells....

20060210980 - Methods of predicting mortality risk by determining telomere length: The present invention provides for methods of determining telomere length of an organism and correlating the measured telomere length with mortality risk associated with telomere length in a population. The presence of shorter telomeres is associated with an increased mortality rate and increased susceptibility to certain types of diseases for...

20060211027 - Methods of treating or preventing a disease, disorder or condition associated with a viral infection: The present invention relates to methods of treating or preventing a disease, disorder or condition associated with a viral infection using a dosing and resting regimen for administering a pharmaceutical composition that provides ARP....

20060211029 - Methods of using dyes in association with nucleic acid staining or detection and associated technology: Methods of using dyes and associated technology are provided. A dye, such as a monomeric dye or a dimeric dye, may be used in a nucleic acid gel staining application and/or a nucleic acid detection application. Such a dye and a salt that comprises an anion that is associated with...

20060211000 - Methods, compositions, and kits for detection of microrna: The present invention provides methods, nucleic acids, compositions, and kits for detecting microRNA (miRNA) in samples. The methods comprise ligating two oligonucleotides together in an miRNA mediated fashion, and detection of the ligation product. The methods can further comprise amplification of the ligation product, such as by PCR. The nucleic...

20060211011 - Methods, devices and kits for multiplex blotting of biological samples from multi-well plates: The present disclosure provides methods, devices and kits that permit large numbers of target biomolecules to be detected simultaneously in samples originating from a multi-sample holder, such as a multi-well plate. One specific example method is a method of making multiple substantial replicas of a biomolecular content of a multi-well...

20060211012 - Mhc class ii as a preventive vaccine against hiv infection: A preventive vaccine for AIDS, suitable for HIV negative persons, is described....

20060210999 - Microarray and method for genotyping shv beta lactamases: The present invention relates to a method for genotyping SHV beta lactamase and a method for determining antibiotic resistance conferred by a SHV-type Extended Spectrum Beta Lactamase of a bacterium. Moreover, the present invention pertains to an oligonucleotide array and a kit for a genotyping based detection of SHV beta...

20060211001 - Microdissection-based methods for determining genomic features of single chromosomes: The present provides a microdissection-based method for identifying a genomic feature present within a visible chromosome region. The method includes steps of: (a) micro-dissecting a single copy of a chromosome to obtain a visible chromosome region; (b) amplifying the visible chromosome region to obtain amplified single chromosome DNA; and (c)...

20060210994 - Microfluidic systems and methods for using microfluidic devices: Microfluidic systems and methods of their use are provided....

20060211025 - Molecular signatures of commonly fatal carcinomas: This invention provides methods, kits, and algorithms for obtaining molecular signatures of cells based on their gene expression profiles. Devices for carrying out molecular signature analysis of unknown samples are also provided....

20060210995 - Nanopore analysis systems and methods of using nanopore devices: Systems and methods for nanopore analysis are provided....

20060211015 - Novel compositions and methods for cancer: The present invention relates to novel sequences for use in diagnosis and treatment of carcinomas, especially lymphoma carcinomas. In addition, the present invention describes the use of novel compositions for use in screening methods....

20060211033 - Novel gene: An isolated nucleic acid molecule mapping to chromosome 16q24.3 and comprising the nucleotide sequence set forth in SEQ ID Numbers: 1 or 3....

20060211037 - Novel mouse cxc chemokine receptor: The present invention pertains to a DNA encoding a polypeptide including an entire sequence of the amino acid sequence as shown by SEQ ID NO: 2 or a partial sequence thereof, or a polypeptide including the polypeptide described above, wherein any of the polypeptides has an activity of a receptor...

20060211031 - Novel proteins and nucleic acids encoding same: The present invention provides novel isolated polynucleotides and small molecule target polypeptides encoded by the polynucleotides. Antibodies that immunospecifically bind to a novel small molecule target polypeptide or any derivative, variant, mutant or fragment of that polypeptide, polynucleotide or antibody are disclosed, as are methods in which the small molecule...

20060211034 - Nucleic acid archiving: This invention is directed to a process for tightly binding nucleic acid to solid phase and corresponding processes for the utilization thereof. Nucleic acid is bound to solid phase matrices exhibiting sufficient hydrophilicity and electropositivity to tightly bind the nucleic acids from a sample. These processes include nucleic acid (double...

20060211018 - Nucleozymes and methods of use: Disclosed herein are nucleozymes and methods of use in the detection and analysis of target polynucleotide sequences....

20060211003 - One component and two component dna pol lll replicases and uses thereof: The invention provides one-component and two-component DNA polymerases, as well as kits comprising the same, and methods of using the same for nucleic amplification and nucleic acid sequencing....

20060210971 - Pectate lyases, nucleic encoding them and methods for making and using them: The invention is directed to polypeptides having pectate lyase (pectinase) activity, polynucleotides encoding the polypeptides, and methods for making and using these polynucleotides and polypeptides. The polypeptides of the invention can be used as pectate lyases to catalyze the beta-elimination or hydrolysis of pectin and/or polygalacturonic acid, such as 1,4-linked...

20060210978 - Proteome-wide mapping of post-translational modifications using endopeptidases: The present invention provides novel endonucleases that site-specifically cleave a post-translationally modified polypeptide at a site of post-translational modification. The present invention further provides methods making and using the endonucleases, including methods of mapping post-translational modifications in the human genome....

20060210989 - Screening process for hydantoin racemases: The present invention relates to a screening process for hydantoin racemases and to novel hydantoin racemases, to the nucleic acid sequences coding therefor and to a proces for mutagenesis. Hydantoin racemases are of interest in connection with the production of enantiomerically enriched amino acids from racemic hydantoins....

20060210975 - Secreted and cytoplasmic tumor endothelial markers: To gain a better understanding of tumor angiogenesis, new techniques for isolating endothelial cells (ECs) and evaluating gene expression patterns were developed. When transcripts from ECs derived from normal and malignant colorectal tissues were compared with transcripts from non-endothelial cells, over 170 genes predominantly expressed in the endothelium were identified....

20060210983 - Signal amplification method for detecting mutated gene: There is provided a signal amplification method for detecting a mutated gene, which can increase the detection sensitivity of mutated genes on a DNA chip according to the PALSAR method, can establish efficient signal amplification and can establish simple detection by contriving design of oligonucleotide probes for use in the...

20060211009 - System for biomarker discovery: The present application discloses a method for discovering a methylation marker gene for the conversion of a cell comprising: (i) comparing converted and unconverted cell gene expression content to identify a gene that is present in greater abundance in the unconverted cell; (ii) treating a converted cell with a demethylating...

20060210977 - Transposon-based vectors and methods of nucleic acid integration: Disclosed herein are compositions comprising integrating enzymes that can deliver nucleic acids to a target DNA. Additionally, the methods of using the compositions disclosed herein relate to treatments for a variety of infections, conditions, and genetic disorders....

20060210984 - Use of nucleic acid mimics for internal reference and calibration in a flow cell microarray binding assay: The present application describes a method for normalizing for variations in signal intensity observed in a biomolecular binding assay carried out in a flow cell cartridge. Variations in signal intensity occur as a result of the effect of the surfaces of a flow cell cartridge on the laminar flow of...

20060211046 - Bacterial ligand-binding sensor: The invention relates to fusion proteins and bacteria encoding them. The fusion proteins include a ligand-binding domain interposed between the splicing domains of an intein. An auxotroph-relieving protein domain is fused to one of the splicing domains so that the auxotroph-relieving function of the domain is activated upon ligand binding....

20060211048 - Biopolymer marker indicative of disease state having a molecular weight of 1211 daltons: The instant invention involves the use of a combination of preparatory steps in conjunction with mass spectroscopy and time-of-flight detection procedures to maximize the diversity of biopolymers which are verifiable within a particular sample. The cohort of biopolymers verified within such a sample is then viewed with reference to their...

20060211040 - Crystal structure of chorismate synthase and uses thereof: The invention describes the identification of the structure coordinates for the enzyme Chorismate Synthase. There is a computer programmed to produce a three-dimensional representation of a molecule or molecular complex, wherein the molecule or molecular complex comprises a binding domain defined by the structure coordinates of (a) Arg 39, His...

20060211049 - Estrogen related receptor, erralpha, a regulator of bone formation: Methods and pharmaceutical compositions are provided for modulating bone formation in a mammal. Methods are also provided for screening compounds for their efficacy as modulators of bone formation....

20060211045 - Fluorescently tagged ligands: characterised in that linking is at same or different linking sites in compounds comprising different Lig, JL, L JT and/or -Tag and is at different linking sites in compounds comprising same Lig, JL, L JT and/or -Tag; process for the preparation thereof; process for the preparation of a library compound...

20060211042 - Methods of identifying modulators of cellular glycosylation using gtrap3-18: The present invention provides methods and compositions for identifying compounds which modulate cellular glycosylation. The invention further provides methods for treating subjects suffering from or at risk of developing a glycosylation associated disorder....

20060211050 - Methods of using 18903 to treat pain and pain-related disorders: The invention provides isolated nucleic acid molecules, designated COE-2 nucleic acid molecules, which encode novel carboxylesterase family members. The invention also provides antisense nucleic acid molecules, recombinant expression vectors containing COE-2 nucleic acid molecules, host cells into which the expression vectors have been introduced, and nonhuman transgenic animals in which...

20060211043 - Molting hormone receptor and method for screening ligand to the receptor: The present invention provides a completely novel molting hormone receptor, which is used for efficiently screening a substance that can be applied to a disinfestant or the like. An insect molting hormone receptor comprising the following polypeptide (a) and polypeptide (b) or (c): (a) a polypeptide having the amino acid...

20060211041 - Protein binding to akt2: A novel polypeptide useful in screening an insulin resistance improving agent and a carbohydrate metabolism improving agent, a polynucleotide coding for the aforementioned polypeptide, an expression vector comprising the aforementioned polynucleotide, and a cell transfected with the aforementioned expression vector are disclosed. The aforementioned polypeptide is a protein which is...

20060211047 - Test kit: It is an object of the present invention to significantly improve the treatment efficiency of bronchial asthma by intervening with inhaled steroids or the like and discontinuing the intervention at the most appropriate times respectively on the basis of accurately and objectively grasping the pathologic condition of an asthma patient...

20060211044 - Translucent solid matrix assay device dor microarray analysis: The present invention concerns methods and compositions relating to translucent matrix arrays. In certain embodiments, the arrays are reconfigurable. Reconfigurable arrays may be produced using small linker molecules, such as aptamers or affibodies, which preferably bind to an IgG specific portion of antibodies. Such arrays may be used to detect...

20060211051 - Methods and reagents for preparing and using immunological agents specific for p-glycoprotein: This invention relates to immunological reagents and methods specific for a mammalian, transmembrane protein termed Pgp, having a non-specific efflux pump activity established in the art as being a component of clinically-important multidrug resistance in cancer patients undergoing chemotherapy. The invention provides methods for developing and using immunological reagents specific...

20060211052 - Screening antibodies using an optical fiber array device capable of simultaneously performing multiple functional assays: Disclosed is a method of screening binding molecules, including Monoclonal Antibodies, using an optical fiber array device. Target cells are coated onto a population of microbeads, wherein each microbead is coated with several cells of the same cellular type and has an assay and an assay reporter associated with it....

20060211053 - Selecting therapeutic human monoclonal antibodies for disease-specific libraries: The invention relates to a method of generating human monoclonal antibodies suitable as therapeutic candidates from a pool of human peripheral blood lymphocytes extracted from a number of individuals who have all been exposed to the same disease antigen. Peripheral blood lymphocytes extracted from said individuals are immortalized, and a...

20060211056 - Leishmania antigens suitable for a diagnostic kit of leishmania: A purified Leishmania infantum polypeptide comprising at least 10 consecutive amino acids of a protein is provided. The protein is mitochondrial integral ADP/ATP carrier protein, NADH-cytochrome b5 reductase, mitochondrial carrier protein, guanine nucleotide binding protein beta subunit (LACK), aldehyde reductase, ubiquinol-cytochrome-c reductase Rieske iron-sulfur protein, truncated elongation factor 1-alpha having...

20060211060 - Biological markers predictive of anti-cancer response to epidermal growth factor receptor kinase inhibitors: The present invention provides diagnostic and prognostic methods for predicting the effectiveness of treatment of a cancer patient with an EGFR kinase inhibitor. Methods are provided for predicting the sensitivity of tumor cell growth to inhibition by an EGFR kinase inhibitor, comprising assessing whether the tumor cell has undergone an...

20060211058 - Diagnosis and treatment of malignant neoplasms: The invention features a method for diagnosing a malignant neoplasm in a mammal by contacting a bodily fluid from the mammal with an antibody which binds to an human aspartyl (asparaginyl) beta-hydroxylase (HAAH) polypeptide and methods of treating malignant neoplasms by inhibiting HAAH....

20060211057 - Methods and compounds for promoting vessel regression: The present invention relates, at least in part, to methods and compositions for treating and diagnosing disorders associated with neovascularization, and methods for identifying targets and compositions used in treating and diagnosing such disorders....

20060211059 - Methods of improving screening, diagnosis and staging of prostate cancer using serum testosterone: The present invention provides methods of screening for, detecting or diagnosing prostate cancer in a subject by determining the level of prostate specific antigen (PSA), complexed PSA (cPSA), free PSA, B-PSA, PRO-PSA or HK2 in a biological sample from the subject and correcting this level for free/bioavailable serum testosterone, total...

20060211062 - Compositions and methods for detection of ehrlichia canis and ehrlichia chaffeensis antibodies: The invention provides compositions and methods for the detection and quantification of Ehrlichia chaffeensis or Ehrlichia canis, Ehrlichia chaffeensis or Ehrlichia canis antibodies, antibody fragments, and polypeptides....

20060211061 - Devices and methods for rapid detection of pathogens: Assay systems and methods are provided for detecting a target pathogen, such as a microorganism (e.g., bacterium, bacterial toxin) which may be present in a fluid or other location. The method can include linking a magnetic microparticle to a first epitope of the target microorganism in a fluid via a...

20060211055 - Capture and release assay system and method: A method for detecting one or more components of interest in a fluid-borne sample in a microchannel of a microfluidic device includes flowing the fluid-borne sample, in which the one or more components of interest are bound to a first labeled component binding moiety to form a first labeled complex,...

20060211054 - Method for determining effective parathormone activity in a sample: Method for determining the active parathyroid hormone content in a sample, wherein the component of parathyroid hormone polypeptide chains which are oxidized at one or more sites near the PTH receptor binding structure having the amino acids 15 to 22 are specifically excluded from the content determination. Oxidation of the...

20060211064 - Detection of hypertension using glucuronidated metabolic products: A method of assessing arachidonic acid (AA) metabolites-dependent hypertension by measuring glucuronidated dihydroxyeicosatrienoic acids (DHETS) and DHET metabolites in a biological sample which contains the epitopes unique to DHET (using any methods including GC/MS, LC/MS or ELISA). An example of the glucuronidated DHET metabolite is DHET-alcohols such as omega or...

20060211065 - Device including a proteinaceous factor, a recombinant proteinaceous factor, and a nucleotide sequence encoding the proteinaceous factor: A device that includes a proteinaceous factor is disclosed. The proteinaceous factor is encoded by the nucleotide sequence of any one of SEQ ID NO.: 1, a degenerate variant of SEQ ID NO.: 1, and a complement of SEQ ID NO.: 1. The proteinaceous factor may be a recombinant. In...

20060211066 - Device including a proteinaceous factor, a recombinant proteinaceous factor, and a nucleotide sequence encoding the proteinaceous factor: A device that includes a proteinaceous factor is disclosed. The proteinaceous factor is encoded by the nucleotide sequence of any one of SEQ ID NO.: 1, a degenerate variant of SEQ ID NO.: 1, and a complement of SEQ ID NO.: 1. The proteinaceous factor may be a recombinant. In...

20060211067 - Device including a proteinaceous factor, a recombinant proteinaceous factor, and a nucleotide sequence encoding the proteinaceous factor: A device that includes a proteinaceous factor is disclosed. The proteinaceous factor is encoded by the nucleotide sequence of any one of SEQ ID NO.: 1, a degenerate variant of SEQ ID NO.: 1, and a complement of SEQ ID NO.: 1. The proteinaceous factor may be a recombinant. In...

20060211068 - Device including a proteinaceous factor, a recombinant proteinaceous factor, and a nucleotide sequence encoding the proteinaceous factor: A device that includes a proteinaceous factor is disclosed. The proteinaceous factor is encoded by the nucleotide sequence of any one of SEQ ID NO.: 1, a degenerate variant of SEQ ID NO.: 1, and a complement of SEQ ID NO.: 1. The proteinaceous factor may be a recombinant. In...

20060211069 - Device including a proteinaceous factor, a recombinant proteinaceous factor, and a nucleotide sequence encoding the proteinaceous factor: A device that includes a proteinaceous factor is disclosed. The proteinaceous factor is encoded by the nucleotide sequence of any one of SEQ ID NO.: 1, a degenerate variant of SEQ ID NO.: 1, and a complement of SEQ ID NO.: 1. The proteinaceous factor may be a recombinant. In...

20060211063 - Phosphokinase assay: A method of detecting protein kinases in a sample by coating a solid surface with a buffer containing protease and phosphatase inhibitors and immobilizing a labeled antibody to the coated solid surface, whereby protein kinases react with the antibody, thereby detecting a presence of the protein kinases. Also provided is...

20060211070 - Polyclonal-polyclonal elisa assay for detecting n-terminus-probnp: A specific and sensitive in vitro ELISA assay and diagnostic test kit is disclosed for determining levels of NT-proBNP protein in a variety of bodily fluids, non-limiting examples of which are blood, serum, plasma, urine and the like. The NT-proBNP ELISA assay test employs the sandwich ELISA technique to measure...

20060211071 - Device for aggregating, imaging and analyzing thrombi and a method of use: An instrument for capturing an image of thrombus formation, blood coagulation, recruitment of circulating inflammatory or tumour cells in a blood sample. The instrument comprises a member defining a channel therethrough, a fluid handling assembly that permits the blood sample to move through the channel at a flow rate, and...

20060211072 - Process, composition and kit for providing a stable whole blood calibrator/control: The present invention is directed toward a stable calibrator and/or control, kit and process for using in a glucose monitoring instrumentation. Principally, the instant invention teaches a glycolyzed red blood cell component which has been treated with a glycolysis stabilizing effective amount of at least one non-crosslinking aldehyde compound which...

20060211073 - Assay for b-raf activity based on intrinsic mek atpase activity: The present invention provides a convenient assay to identify compounds with B-Raf inhibitory activity, referred to as the BRAMA (B-Raf Accelerated MEK ATPase) assay. The BRAMA assay is based on the discovery of ATPase activity of MEK, and utilizes changes in NADH concentration over time as an indicator of the...

20060211075 - Enzyme sensors including environmentally sensitive or fluorescent labels and uses thereof: Sensors for detecting enzyme activity are provided. The sensors include substrate modules having environmentally sensitive labels and detection modules whose binding to the substrate modules results in changes in signals from the environmentally sensitive labels or polypeptides or polypeptide substrates including environmentally sensitive or fluorescent labels. Compositions including substrate modules,...

20060211074 - Kinase-directed, activity-based probes: Various embodiments of the present invention are directed to kinase-directed, activity-based probes (“KABPs”) that tightly bind to, and label, kinases. Each KABP includes a binding group that is recognized and bound by one or more kinases, a reactive group that tightly, and generally irreversibly, binds to the kinase, a tag...

20060211078 - Caspase 9 activation and uses therefor: The present invention discloses methods for activating Caspase 9 in such a way that it can be used in assays to discover modulators of Caspase 9....

20060211076 - Method and device for the qualitative and/or quantitative determination of a protein and/or peptide pattern of a fluid sample, which has been taken from a human or animal body: Provided are a process and a device for the qualitative and/or quantitative determination of a protein and/or peptide pattern of a liquid sample taken from a human or animal body for checking its state. The peptides and proteins of the liquid sample are processed and then subjected to analysis, wherein...

20060211077 - Methods and compositions to diagnose disease using concatenated oligopeptide standard: The present invention is a method to quantitate proteins associated with a disease or disease state comprising the steps of isolating at least two target proteins from a test sample, combining the target proteins with a known concentration of a concatenated oligopeptide standard and a proteinase to form peptide fragments...

20060211079 - Systemic markers for asthma and analogous diseases: Provided herein are diagnostic and prognostic methods, diagnostic and prognositic markers, and methods for evaluating anti-inflammatory agents or drugs in subjects with asthma and/or an analogous disease associated with high oxidative and nitrative stress at the disease site. In certain embodiments, the methods comprise a step of assaying for decreased...

20060211080 - Automated biological plate spreader: The present invention relates to a robotic apparatus and method for automating the spreading of a biologic sample on a plate, wherein the robot includes at least one effector arm to hold and shake the plate and a pipetting arm to deliver the sample to the plate. The present invention...

20060211082 - Methods and compositions for rapidly detecting and quantifying viable legionella: Methods and compositions detect and quantify viable Legionella. Dip-slides that include an absorbent medium, growth promoting, and growth selective substances are useful in rapid detection and quantification of microcolonies of Legionella. Most probable number method of detection and quantification of Legionella are disclosed....

20060211081 - Sec14 as an antifungal target: The invention provides SEC14 cytosolic factor (SEC14) as a novel antifungal target, screening methods for SEC14 inhibitors and their use as antifungal compounds, pharmaceutical compositions containing them and their use in medicine....

20060211083 - Products and processes for in vitro synthesis of biomolecules: Provided herein are products and processes for efficiently synthesizing biomolecules in vitro using cell-free extracts derived from mammalian cells and insect cells. In an embodiment, provided is a process for preparing a cell-free extract from insect cells and mammalian cells that efficiently synthesizes post-translationally modified target proteins (e.g., glycosylated target...

20060211087 - Compositions and methods for producing recombinant proteins: A class of integral membrane proteins, referred to as Mistic polypeptides, their variants, fusion proteins including a Mistic polypeptide domain, and nucleic acid molecules encoding Mistic polypeptides and Mistic fusion proteins are disclosed herein. Also described are methods of using Mistic polypeptides and Mistic fusion proteins to produce and/or isolate...

20060211089 - Expression of defensins in filamentous fungi: The present invention relates to recombinant expression of defensin antimicrobial peptides in fermentation of filamentous fungi....

20060211094 - Glucose dehydrogenase beta subunit and dna encoding the same: A DNA fragment encoding a β subunit is obtained by inverse PCR using primers designed based on the nucleotide sequnece of a N-terminal signal sequence region of a GDH β subunit derived from Burkholderis cepacia KS1 strain....

20060211090 - Human cdnas and proteins and uses thereof: The invention concerns GENSET polynucleotides and polypeptides. Such GENSET products may be used as reagents in forensic analyses, as chromosome markers, as tissue/cell/organelle-specific markers, in the production of expression vectors. In addition, they may be used in screening and diagnosis assays for abnormal GENSET expression and/or biological activity and for...

20060211091 - Mage-a3 peptides presented by hla class ii molecules: The invention describes HLA class II binding peptides encoded by the MAGE-A3 tumor associated gene, as well as nucleic acids encoding such peptides and antibodies relating thereto. The peptides stimulate the activity and proliferation of CD4+ T lymphocytes. Methods and products also are provided for diagnosing and treating conditions characterized...

20060211093 - Membrane scaffold proteins and embedded membrane proteins: Membrane proteins are difficult to express in recombinant form, purify, and characterize, at least in part due to their hydrophobic or partially hydrophobic properties. Membrane scaffold proteins (MSP) assemble with target membrane or other hydrophobic or partially hydrophobic proteins or membrane fragments to form soluble nanoscale particles which preserve their...

20060211092 - Membrane scaffold proteins and tethered membrane proteins: Membrane proteins are difficult to express in recombinant form, purify, and characterize, at least in part due to their hydrophobic or partially hydrophobic properties. Membrane scaffold proteins (MSP) assemble with target membrane or other hydrophobic or partially hydrophobic proteins or membrane fragments to form soluble nanoscale particles which preserve their...

20060211088 - Method for generating variable domain sequences of heavy chain antibodies: The present invention relates to a method for generating or cloning a nucleic acid or nucleotide sequence that encodes a heavy chain antibody or an antigen-binding fragment thereof, wherein said heavy chain antibody or antigen-binding fragment is directed against a specific antigen, said method comprising the steps of providing a...

20060211085 - Methods for reducing or eliminating alpha-mannosidase resistant glycans for the production of glycoproteins: The present invention provides methods to reduce or eliminate α-mannosidase resistant glycans on glycoproteins in yeast. The reduction or elimination of α-mannosidase resistant glycans on glycoproteins results from the disruption of the newly isolated P. pastoris AMR2 gene encoding β1,2-mannosyltransferase. The present invention also discloses novel genes, polypeptides, antibodies, vectors...

20060211086 - Process for manufacture of nematode-extracted anticoagulant protein (nap): The present invention provides a process for manufacture of purified Nematode-extracted Anticoagulant Proteins (NAPs), wherein the NAP manufactured by the claimed process method is a NAP drug substance that can be formulated as a NAP drug product. The present invention provides NAP drug substances and NAP drug products manufactured by...

20060211084 - Simplified eukaryotic nitrate reductase: The invention provides modification to the polynucleotide coding sequence for Pichia angusta NAD (P)H: nitrate reductase [YNaR1; GenBank accession number Z49110], which has Enzyme Commission number 1.7.1.2 (formerly EC 1.6.6.2), yielding the polynucleotide coding sequence for simplified eukaryotic nitrate reductase (S-NaR1). The invention also provides a method for recombinant expression...

20060211095 - Process for the fermentative production of s-adenosylmethionine: A microorganism strain secreting S-adenosylmethionine having increased activity of the cmr (mdfA) gene product is provided....

20060211096 - Enzyme catalysis in the presence of ionic liquids: The invention relates to the implementation of enzyme-catalysed reactions in the presence of ionic liquids....

20060211097 - Process for producing enantiopure beta-amino acid derivatives, and enantiopure beta-amino acid derivatives: Process for producing enantiopure β-amino acid derivatives corresponding to general formula (I) R1-NZ-CHR2—CH2—COOR3 (I) in which R1 and R2 independently denote organic residues optionally forming a cyclic substituent, R3 denotes H or an organic residue, and Z represents H or an amino function-protecting group, comprising a step in which a...

20060211098 - Cells overexpressing lipoyl-protein ligase b-gene for fermentative production of r-alpha-liponic acid: The invention relates to cells and to a method for the production of R-α-liponic acid by fermentation. The inventive host organism strain, which is suitable for fermentative production of R-α-liponic acid, is characterized in that it overexpresses a gene coding for lipoyl-protein ligase B and in that it releases the...

20060211099 - L-carnitin dehydrogenases, their derivatives and method for producing substituted (s) alkanols: The present invention relates to proteins having an enzymatic activity of reducing substituted alkanones such as 3-methylamino-1-(2-thienyl)-propan-1-one. The invention furthermore relates to nucleic acids coding for said proteins, nucleic acid constructs, vectors, genetically modified microorganisms and to methods for preparing substituted (S)-alkanols, such as, for example, (S)-3-methylamino-1-(2-thienyl)-(S)-propanol....

20060211100 - Novel polyhydroxy alkanoate copolymer including within molecule unit having vinyl group or carboxyl group in side chain, and producing method therefor: The invention provides a PHA copolymer including at least a 3-hydroxy-ω-carboxyalkanoic acid represented by a formula (19) or (32) and simultaneously at least a unit represented by a formula (2) or a formula (3) in a molecule, a precursor PHA copolymer having a corresponding vinyl group or a corresponding alkoxycarbonyl...

20060211101 - Methods for producing end-products from carbon substrates: The present invention provides means for the production of desired end-products of in vitro and/or in vivo bioconversion of biomass-based feed stock substrates, including but not limited to such materials as starch and cellulose. In particularly preferred embodiments, the methods of the present invention do not require gelatinization and/or liquefaction...

20060211102 - Human lysophosphatidic acid acyltransferase gamma-1 polypeptide: Polypeptides are obtained, for example, via expression of encoding cDNA sequences, that have the activity of the enzyme lysophosphatidic acid acyltransferase (LPAAT), also known as 1-acyl sn-glycerol-3-phosphate acyltransferase....

20060211103 - Thermostable reverse transcriptases and uses thereof: The present invention is in the fields of molecular and cellular biology. The invention is generally related to reverse transcriptase enzymes and methods for the reverse transcription of nucleic acid molecules, especially messenger RNA molecules. Specifically, the invention relates to reverse transcriptase enzymes which have been mutated or modified to...

20060211104 - Hyaluronan synthases and methods of making and using same: A functionally active hyaluronan synthase having at least one modified amino acid residue therein as compared to a corresponding functionally active native hyaluronan synthase such that the functionally active hyaluronan synthase has an altered enzymatic activity as compared to the corresponding functionally active native hyaluronan synthase is disclosed. Methods of...

20060211105 - Rhodococcus erythropolis lg12 having acrylic acid degrading activity and method for removing acrylic acid using the same: The present invention relates to a novel strain, Rhodococcus erythropolis LG12, having acrylic acid degrading activity and resistance to acrylic acid, and a method for removing acrylic acid from acrylic acid-containing contaminants using the same. The strain according to the invention has not only acrylic acid degrading activity but also...

20060211106 - Test specimen and production thereof: A test specimen is provided which has one or more chemical substances fixed to prescribed plural independent positions on a substrate, and the quantities of the chemical substances fixed at the respective prescribed positions are the total of integer multiples of existence quantity units defined for the respective chemical substances...

20060211107 - Protease inhibitor conjugates and antibodies useful in immunoassay: Activated haptens useful for generating immunogens to HIV protease inhibitors, immunogens useful for producing antibodies to HIV protease inhibitors, and antibodies and labeled conjugates useful in immunoassays for the HIV protease inhibitor saquinavir. The novel haptens feature an activated functionality at the central, non-terminal hydroxyl group. Also described are monoclonal...

20060211108 - Methods and compositions for impairing multiplication of hiv-1: A single isolated antibody or antibody fragment thereof binds to multiple variant sequences within an epitope of HIV-1 Tat protein displayed in multiple strains and subtypes of HIV-1. This “pan-epitope” antibody is useful in therapeutic and prophylactic compositions and treatments of HIV-1 infection, regardless of strain. This pan-epitope antibody is...

20060211109 - Derivation of terminally differentiated dopaminergic neurons from human embryonic stem cells: The present disclosure is directed to improved methods for efficiently producing neuroprogenitor cells and differentiated neural cells such as dopaminergic neurons and serotonergic neurons from pluripotent stem cells, for example human embryonic stem cells. Using the disclosed methods, cell populations containing a high proportion of cells positive for tyrosine hydroxylase,...

20060211110 - Human trophoblast stem cells and use thereof: Existence of human trophoblast stem (hTS) cells has been suspected but unproved. The isolation of hTS cells is reported in the early stage of chorionic villi by expressions of FGF4, fgfr-2, Oct4, Thy-1, and stage-specific embryonic antigens distributed in different compartments of the cell. hTS cells are able to derive...

20060211111 - Compositions and methods for neural cell production and stabilization: The present invention provides compositions and methods for mammalian neural cell production, their stabilization and their proliferation. More particularly, the present invention provides cellular differentiation methods employing culturing the cells on a cell line or in cell culture and further contacting the cells with MEDII conditioned medium for the generation...

20060211112 - Growth of human dendritic cells for cancer immunotherapy in closed system using microcarrier beads: A method and apparatus for reproducibly generating dendritic cells are provided. Blood mononuclear cells are loaded into a cell culture container containing microcarrier beads therein. Tissue culture comprising the cells loaded in the container is incubated for a predetermined period. Nonadherent cells and cells adhered to the beads are separated....

20060211113 - Method and system for introducing a gene into a human stem cell: A method and vector system for delivering of a gene into a human stem cell for therapeutic uses is disclosed. The method includes linking a therapeutic gene to human sperm cells through a linker and fertilizing a human oocyte. The resulting zygote may then be cultured and established as human...

20060211114 - Modulation of hedgehog-mediated signaling pathway: The present invention provides transcription factors associated with the hedgehog signaling pathway that are regulated by dephosphorylation by phosphatases. Hedgehog response elements (HRE) that interact with the dephosphorylated transcription factors are also provided as well as methods for identifying compounds that are phosphatase inhibitors. Methods of treating tumors in a...

20060211115 - Methods of generating chimeric adenoviruses and uses for such chimeric aden oviruses: A method for providing an adenovirus from a serotype which does not grow efficiently in a desired cell line with the ability to grow in that cell line is described. The method involves replacing the left and right termini of the adenovirus with the corresponding termini from an adenovirus which...

20060211116 - Transposase and method of gene modification: A transposase encoded by the Tol2 element; a polynucleotide encoding the same; a method of modifying the gene structure of a cell (preferably a vertebrate cell) by using the above protein; a method of modifying the function of a cell by modifying the gene structure thereof; and a cell having...

20060211118 - compositions and methods using the yeast ymr107w promoter: The invention provides novel yeast promoters useful for controlling the expression of homologous and heterologous nucleic acid molecules in yeast cells. The yeast promoters are induced by a fermentable carbon source, such as glucose, or a non-fermentable carbon source, such as ethanol, or both. Therefore, expression of nucleic acid molecules...

20060211117 - Methods of making minicircles: The present invention relates to a method for the production of a minicircle. In the method of the present invention, a parent plasmid is provided which has a nucleic sequence flanked by recombination sites. This parent plasmid is exposed to an enzyme which causes recombination at the recombination sites, thereby...

20060204950 - Biological sample preservation, transportation and storage device: The biological transport and storage device includes a cooling chamber containing one or more biological samples in a sample holder surrounded by coolant air which is intermittently circulated through the cooling chamber across a thermoelectric unit that can cool or heat the circulating air. A fan is used to circulate...

20060204953 - Method and apparatus for automated analysis of biological specimen: Apparatus and methods for automatic classification of cells in biological samples is disclosed. Aggregation parameters of identified objects are calculated using relative spatial positions of the objects, and the aggregation parameters are used in the classification. Centres of cells may be located using an edge detector and parameterisation across at...

20060204951 - Platelet biomarkers for the detection of disease: The present inventors have surprisingly discovered that platelets sequester angiogenic regulators and prevent their degradation. Thus, by analyzing levels of angiogenic regulators in platelets, it is now possible to detect angiogenic activity, even at an early stage. By monitoring for changes in angiogenic activity, the presence of cancer or other...

20060204952 - Technique for measuring cell-membrane properties in cultured cells grown on biomaterials in an ussing chamber: The present invention is directed to a system and method which measures transcellular parameters in which cells are cultured in a monolayer on a biomaterial. The parameters measured are indicative of in vivo values such as cell-cell and cell-matrix adhesion parameters....

20060204954 - Compositions and methods for binding agglomeration proteins: Amplibody compositions and methods used to interact with agglomeration proteins are disclosed herein. Compositions herein comprise one or more DNA or RNA molecules having affinity for at least one agglomeration protein. The nucleic acid component is a naturally or non-naturally occuring molecule with twenty or more ribonucleotide bases. For RNA,...

20060204955 - Method for the production of bacterial toxins: Methods and compositions are provided for the enhanced production of bacterial toxins in large-scale cultures. Specifically, methods and compositions for reducing bacterial toxin expression inhibitors are providing including, but not limited to, addition of toxin expression inhibitor binding compounds, culture media having reduced concentrations of toxin inhibitor metabolic precursors and...

20060204998 - Alleles of the human mu opioid receptor, diagnostic methods using said alleles, and methods of treatment based thereon: Provided herein are variant alleles of a gene encoding a mu opioid receptor, along with cloning vectors for replicating such variant alleles, expressing vectors for expressing the variant alleles to produce variant mu opioid receptors, and antibodies to such variant receptors. Also disclosed are binding characteristics of such variant receptors...

20060204987 - Applications with and methods for producing selected interstrand cross-links in nucleic acids: The invention provides a method for stably cross-linking a first nucleic acid sequence selected for being stably cross-linked in the presence of a second nucleic acid sequence selected for not being stably cross-linked in a nucleic acid molecule. The method comprises providing a nucleic acid sequence that is complementary to...

20060205005 - Artificial red blood cells: An artificial red blood cells are disclosed comprising a thin film encapsulating a core, the core comprising hemoglobin, wherein the film comprises a plurality of layers of polypeptides, opposing layers comprising alternating oppositely charged polypeptides, and wherein at least half of the amino acid residues in the oppositely charged polypeptides...

20060204965 - Associations of polymorphisms in the frbz gene in obesity and osteoporosis: Methods and reagents for determining sequence variants present at the FRZB locus, which facilitate identifying individuals at risk for obesity and/or osteoporosis, are provided. In particular, methods for detecting an individual's risk for obesity and/or osteoporosis by detecting at least one polymorphism (e.g., at least one single nucleotide polymorphism) in...

20060204983 - Binding assay using binding agents with tail groups: The present invention discloses methods and kits for the determination of the concentration of one or more analytes in a liquid sample using capture agents immobilised on a solid support and binding agents for binding the analyte(s), the binding agents having tail groups capable of binding to the respective capture...

20060204970 - Chip-based speciation and phenotypic characterization of microorganisms: This invention provides oligonucleotide based arrays and methods for speciating and phenotyping organisms, for example, using oligonucleotide sequences based on the Mycobacterium tuberculosis rpoB gene. The groups or species to which an organism belongs may be determined by comparing hybridization patterns of target nucleic acid from the organism to hybridization...

20060204980 - Colorectal cancer therapies: Methods related to the targeted treatment of p53+ colorectal cancer cells, and methods of screening for new anti-tumor agents, are described....

20060204989 - Comparative analysis of extracellular rna species: The invention provides methods and kits for enabling quantitative or qualitative analysis of extracellular RNA species in non-cellular bodily fluids including plasma and serum to detect, infer, evaluate, or monitor cancer and other neoplasia or other diseases of interest....

20060204981 - Compositions for modulation of parp and methods for screening for same: The present invention relates a method for screening for a PARP activator. The screening method comprises the step of assessing the PARP-activating effect of a test compound, using cells, cell lysate, or purified PARP. The present invention also provides a method for the treatment of cancers. The treatment method comprises...

20060204990 - Compounds and methods for fluorescent labeling: Compounds useful in labeling biological materials are provided along with methods for their use....

20060204977 - Cross reactive arrays of three-way junction sensors for steroid determination: This invention provides analyte sensitive oligonucleotide compositions for detecting and analyzing analytes in solution, including complex solutions using cross reactive arrays of analyte sensitive oligonucleotide compositions....

20060204999 - Detecting molecular complexes: The invention provides methods and compositions for detecting the presence of, or for measuring amounts of, molecular complexes, particularly complexes comprising two or more proteins, such as receptor complexes of cell surface membranes. In one aspect of the invention, reagent pairs are provided that comprise a cleaving probe that specifically...

20060205007 - Dna supporting fiber and dna supporting fiber sheet and methods of producing them: There is provided a DNA supporting fiber capable of maintaining the stability of DNA and efficiently expressing the adsorption property of DNA. Also provided is a DNA supporting sheet useful in a variety of applications, the sheet that utilizes the fiber. The DNA supporting fiber is produced by fusing and...

20060204962 - Genetic polymorphisms as predictive diagnostics for adverse drug reactions (adr) and drug efficacy: The invention provides diagnostic methods and kits including oligo and/or polynucleotides or derivatives, including as well antibodies determining whether a human subject is at risk of getting adverse drug reaction after statin therapy or whether the human subject is a high or low responder or a good a or bad...

20060204969 - Genetic variations (snps) adjacent to the akt1 gene locus, and diagnostic and prognostic uses thereof: We have identified and isolated a 12 kb region immediately upstream of the AKT1 gene containing eight single nucleotide polymorphic polynucleotides (SNPs) in 4 haplotype regions that show strong association with body composition, Basal Mass Index, and AKT1 expression enhancement in human males. A four-locus haplotype (Haplotype 2) was defined...

20060204986 - Immunogen: The present invention relates, in general, to an immunogen and, in particular, to an immunogen for inducing antibodies that neutralize a wide spectrum of HIV primary isolates. The invention also relates to a method of inducing anti-HIV antibodies using same....

20060204975 - Markers for cyclin dependent kinase inhibitors: The present invention relates to pharmacodynamic markers for CDKIs including the candidate 2,6,9-tri-substituted purine known as roscovitine. The identity of these markers facilitates the convenient identification of roscovitine-like activity both in vitro and in vivo....

20060204956 - Method for detection of htr and htert telomerase-associated rna in plasma or serum: This invention relates to methods of detecting extracellular hTR RNA and hTERT RNA in blood, plasma, serum, and other bodily fluids. The invention thereby provides an aid for the detection, diagnosis, monitoring, treatment, or evaluation of neoplastic disease....

20060204992 - Method for determining hair cycle markers: The invention relates to a method for determining hair cycle markers in vitro, test kits and biochips which are used to determine hair cycle markers and to the use of proteins, mRNA molecules, proteins or fragments thereof as hair cycle markers. The invention also relates to a test method which...

20060204988 - Method for determining the methylation pattern of a polynucleic acid: Particular aspects relate to a method for determining the methylation pattern of a polynucleic acid, comprising: a) preparing a solution comprising a mixture of fragments of the polynucleic acid; b) coupling the fragments with a substance being detectable with a detection method; c) contacting a solution comprising the fragments of...

20060204960 - Method for diagnosing diffuse-type gastric cancers: Objective methods for detecting and diagnosing diffuse-type gastric cancer (DGC) are described herein. In one embodiment, the diagnostic method involves the determining an expression level of DGC-associated gene that discriminate between DGC and normal cell. The present invention further provides methods of screening for therapeutic agents useful in the treatment...

20060204997 - Method for performing multi-formatted assays: An automated analyzer for performing multiple diagnostic assays simultaneously includes multiple stations in which discrete aspects of the assay are performed on fluid samples contained in sample vessels. The analyzer includes stations for automatically preparing a sample, incubating the sample, preforming an analyte isolation procedure, ascertaining the presence of a...

20060204996 - Method of designing primer and probe sets, primer and probe set designed by the method, kit comprising the sets, computer readable medium recorded thereon program to execute the method, and method of identifying target sequence using the sets: A method of designing primer and probe sets for identification of target sequences by amplification and hybridization is provided. Also, primer and probe sets designed by the method, a kit comprising the sets, a computer readable medium recorded thereon a program to execute the method, and a method of identifying...

20060204995 - Method of designing probe set, probe set designed by the method, microarray comprising the probe set, computer readable medium recorded thereon program to execute the method, and method of identifying target sequence using the probe set: A method of designing a probe set for identification of target sequences is provided. Also, a probe set designed by the method, a microarray including the probe set, a computer readable medium recorded thereon a program to execute the method, and a method of identifying target sequences using the probe...

20060204985 - Method of hybridizing genes: m

20060204974 - Methods and agents for regulating angiotensin activity: In certain aspects, the present invention relates to methods and preparations for treating angiotensin II-mediated diseases, and in particular, methods of screening for agents that modulate post-transcriptional regulation of angiotensin II receptors and the use of such agents....

20060204984 - Methods and compositions for aggregant detection: This invention relates to an aggregation sensor useful for the detection and analysis of aggregants in a sample, and methods, articles and compositions relating to such a sensor. The sensor comprises first and second optically active units, where energy may be transferred from an excited state of the first optically...

20060204993 - Methods and compositions for modulating chk2 pathways and methods for screening for same: The present invention relates to a method for screening for an activator of Chk2. The screening method comprises assessing the Chk2-activating effect of a test compound in cells containing DNA encoding Chk2. The present invention also provides a method for the treatment or prevention of pre-cancers, cancers, or hyperproliferation disorders....

20060205002 - Methods for determining plasma free drug concentration by direct measurement of binding affinity of protease inhibitors to plasma proteins: Methods for isothermal titration calorimetry analysis of the binding affinity of protease inhibitors to plasma proteins. A method that can quantitatively calculate free drug concentrations of protease inhibitors in human plasma, as well as a method to calculate therapeutic amounts and dosage regimens. Furthermore, the present invention provides a method...

20060204958 - Methods for diagnosing htlv-i-mediated diseases: Protein biomarkers that may advantageously be utilized in aiding in, or making, a diagnosis of HTLV-I-associated myelopathy (HAM), adult T-cell leukemia (ATL) or a negative diagnosis are described. Accordingly, in one aspect of the invention, methods for aiding in, or otherwise making, a diagnosis of ATL, HAM or a negative...

20060204967 - Methods for identifying risk of breast cancer and treatments thereof: Provided herein are methods for identifying risk of breast cancer in a subject and/or a subject at risk of breast cancer, reagents and kits for carrying out the methods, methods for identifying candidate therapeutics for treating breast cancer, and therapeutic methods for treating breast cancer in a subject. These embodiments...

20060204978 - Methods for isolation of nucleic acids from prokaryotic spores: The invention provides methods and related products for extracting nucleic acids such as DNA from prokaryotic spores. The invention also encompasses methods for identifying the source of such spores via analysis of the isolated nucleic acids....

20060204973 - Methods for synthesizing polynucleotides using a single primer: An objective of the present invention is to provide methods for synthesizing polynucleotides comprising an unknown nucleotide sequence in a region containing their 5′-end. By using a single gene-specific primer, nucleotide sequences complementary to each other are added respectively at the 5′- and 3′-ends of a desired polynucleotide, to synthesize...

20060205003 - Methods, systems, and software for identifying functional biomolecules: The present invention generally relates to methods of rapidly and efficiently searching biologically-related data space. More specifically, the invention includes methods of identifying bio-molecules with desired properties, or which are most suitable for acquiring such properties, from complex bio-molecule libraries or sets of such libraries. The invention also provides methods...

20060204964 - Molecular detection systems utilizing reiterative oligonucleotide synthesis: The present invention provides methods for detecting the presence of a target molecule by the use of nucleotide analogs containing moieties that enable detection. Such analogs may be incorporated into nucleic acids. In one embodiment, nucleotide analogs are used in a process generating multiple detectable oligonucleotides through reiterative enzymatic oligonucleotide...

20060204957 - Mta1s, a steriod hormone receptor corepressor: The present invention concerns the use of methods and compositions to diagnose, treat and identify therapeutic molecules for cancer, in particular hormone insensitive cancers. The invention includes polypeptides and nucleic acids encoding polypeptides of MTA1s, a novel protein derived from alternative splicing of the MTA1 gene. Other embodiments include antibody...

20060204976 - Multivalent avian influenza vaccines: A vaccine composition and method which is effective in preventing or ameliorating Avian Influenza Virus infection is set forth herein. The vaccine contains at least two inactivated strains of avian influenza virus, wherein the combined haemoagglutinin (HA) total is at least about 200 HA/dose of the vaccine composition, and wherein...

20060204982 - Novel compositions and methods for cancer: The present invention relates to novel sequences for use in diagnosis and treatment of carcinomas, especially lymphoma carcinomas. In addition, the present invention describes the use of novel compositions for use in screening methods....

20060204994 - Novel human protein kinases and protein kinase-like enzymes: The present invention relates to kinase polypeptides, nucleotide sequences encoding the kinase polypeptides, as well as various products and methods useful for the diagnosis and treatment of various kinase-related diseases and conditions. Through the use of a bioinformatics strategy, mammalian members of the PTK's and STK's have been identified and...

20060204991 - Nucleic acid affinity columns: This invention provides nucleic acid affinity matrices that bear a large number of different nucleic acid affinity ligands allowing the simultaneous selection and removal of a large number of preselected nucleic acids from the sample Methods of producing such affinity matrices are also provided In general the methods involve the...

20060204963 - Nucleotide sequences of coryneform bacteria coding for proteins involved in l-serine metabolism and method for producing l-serine: The invention relates to the nucleotide sequences of coryneform bacteria coding for proteins which are involved in L-serine metabolism with reduced and switched off L-serine dehydratase. Said invention also relates to micro-organisms and to methods for producing L-serine....

20060204971 - Oligonucleotides for multiplexed binding assays: An assay system for an analyte, the system having a solid support; and six or more pairs of oligonucleotides, each oligonucleotide of the pair being at least partially complementary to the other oligonucleotide of the pair. Each pair has a first oligonucleotide and a second oligonucleotide, the first oligonucleotide being...

20060205006 - Pcr method and related apparatus: A method for balancing multiplexed PCR methods is provided. In the method, two or more sequential temporal PCR stages are used to effectively separate two or more PCR reactions in a single tube as an alternative to primer limiting to modulate the relative rate of production of a first amplicon...

20060205000 - Processes for purifying and for detecting target double-stranded dna sequences by triple helix interaction: The present invention relates to novel target double-stranded DNA sequences capable of interacting with a third strand and of forming a stable triple helix. The present invention also relates to a process for purifying a double-stranded DNA molecule, according to which a solution containing said DNA molecule is brought into...

20060204961 - Reagents and methods for diagnosis of attention deficit hyperactivity disorder: The subject invention provides reagents and methods for diagnosing Attention Deficit Hyperactivity Disorder (ADHD) in an individual based on the presence of specific alleles of the DRD4 gene or other markers found within a region of strong linkage disequilibrium to the DRD4 7R allele in the DNA of the individual....

20060204979 - Reverse two-hybrid system for identification of interaction domains: The present invention provides methods for producing allele libraries and vectors for producing these libraries. The present invention also provides methods of identifying interaction domains between proteins. The vectors, kits, and methods of the present invention suitably utilize recombinational cloning to efficiently generate and screen full-length mutant alleles of target...

20060205004 - Reversible association of nucleic acid with a carboxylated substrate: The present invention is directed to methods and compositions wherein nucleic acids are associated with a solid phase that comprises a carboxylated substrate. In specific embodiments, precipitation of the nucleic acids occurs in the absence of salt....

20060204959 - Solid support having electrostatic layer and use thereof: It is intended to provide a solid support capable of immobilizing nucleic acid molecules in a high proportion, and with a high bond strength to nucleic acid molecules. The solid support comprises a substrate and, provided thereon, an electrostatic layer for electrostatically attracting nucleic acid molecules and functional groups capable...

20060204972 - Systems and methods for determining real-time pcr cycle thresholds using a rotation transformation: Systems and methods for determining the elbow or Ct value in a real-time, or kinetic, PCR amplification curve data set. The PCR data set may be visualized in a two-dimensional plot of fluorescence intensity vs. cycle number. A rotation transform is applied to the data set to rotate the data...

20060204968 - Tools for diagnostics, molecular definition and therapy development for chronic inflammatory joint diseases: The invention relates to tools for the diagnosis, molecular definition and development of treatment of chronic inflammatory joint diseases and other inflammatory, infectious or tumourous diseases. According to the invention, genome data (genomics), proteome data (proteomics) and immunome data (immunomics) are used in the analysis and development of treatment of...

20060205001 - Transfusion registry and exchange network: Disclosed is a registry for candidate transfusion donors, which invokes an inventory management policy to create and actively manage lists of candidate donors in order to minimize imbalances between demand and supply across multiple regions and across multiple categories of donors and recipients. Together with a genotyping laboratory, the registry...

20060204966 - Treatment of cancers expressing p95 erbb2: The truncated ErbB2 receptor (p95ErbB2) is shown to differ from the full-length ErbB2 receptor in its association with other ErbB receptors. The truncated receptor preferentially associated with ErbB3, whereas full length ErbB2 heterodimerizes with either EGFR or ErbB3. Consistent with p95ErbB2 heterodimerization with ErbB3, it is shown that heregulin (an...

20060205008 - Use of hepatitis b x-interacting protein (hbxip) in modulation of apoptosis: Novel methods of regulating cellular apoptosis by affecting the interaction of hepatitis B X-interacting protein (HBXIP) with Survivin are described. More specifically, these novel methods of enhancing apoptosis of neoplastic cells comprises inhibiting interaction of hepatitis B X-interacting protein (HBXIP) with Survivin....

20060205011 - Combinatorial artificial receptors including tether building blocks on scaffolds: The present invention relates to artificial receptors on scaffolds, methods of and compositions for making them, and methods of using them. Each artificial receptor includes a plurality of building block compounds coupled to a scaffold. In an embodiment, at least one of the building blocks includes a tether moiety. The...

20060205012 - Diagnostic test: Disclosed are methods for conducting diagnostic tests for the detection of the inflammatory bowel diseases, such as Crohn's disease and ulcerative colitis. Also described are methods for monitoring a patient by administering tests of the present invention. Also described are methods for monitoring patient's treatment by administering tests of the...

20060205013 - Fet-type biosensor with surface modification: Provided is a field effect transistor (FET) type biosensor including a source electrode, a gate, and a drain electrode. A ligand that can bind to a side of a nucleic acid is added to the surface of the gate. In a conventional FET type biosensor, it is difficult to detect...

20060205015 - Method for detecting the interactions between a g protein-coupled receptor (gpcr) and one of the galpha or gbetagamma subunits: The present invention relates to a method for the detection of ligands (agonists or antagonists) specific for a G protein-coupled receptor (GPCR), which comprises the steps consisting in: 1) bringing a receptor labelled with a member of a donor/acceptor pair into contact with a Gα or Gβγ subunit of a...

20060205014 - Method for diagnosing and prognosing inflammatory bowel disease and crohn's disease: Disclosed are methods for diagnosing and prognosing Inflammatory Bowel disease or Crohn's disease (CD) by measuring levels of antibodies to glycans in a biological sample....

20060205019 - Methods and compositions for directed gene assembly: The present invention is directed to methods and compositions for use of homologous recombination for directed evolution, gene reassembly, and directed mutagenesis. One aspect of the present invention relates to methods for use of bacterial conjugative transfer and homologous recombination for directed evolution, gene reassembly, and directed mutagenesis. Another aspect...

20060205009 - Methods of assessing endothelial dysfunction using acute transient responese following fat administration: The present invention provides methods utilizing the fat-induced antibody response (FIAR) to assess endothelial function. Additional methods are provided that utilize FIAR for diagnosing and monitoring the progression of vascular diseases, the success of treatment for these diseases, and as a measure of the oxidative stress imposed upon vascular endothelium....

20060205010 - Methods of host cell protein analysis: The invention provides methods of determining the presence of host cell proteins in sample. The invention further provides methods of following the purification of target proteins. Kits and articles of manufacture are also provided....

20060205017 - Microorganism genomics, compositions and methods related thereto: The present invention provides methods and compositions for accessing, in a generally unbaised manner, a diverse genetic pool for genes involved in biosynthetic pathways. The invention also provides compounds which can be identified by cloning biosynthetic pathways....

20060205018 - Novel human enzymes of the metalloprotease family: This invention relates to newly identified polypeptides which have zinc metalloprotease activities and are referred to as IGS5, and polynucleotides encoding such polypeptides, to their use in therapy and in identifying compounds which may be stimulators and/or inhibitors which are useful in therapy, and to production of such polypeptides and...

20060205016 - Protein a based binding domains with desirable activities: Provided are Staphylococcal protein A (SpA) variants for binding immunoglobulin (Ig), comprising a polypeptide which varies by one or more amino acids from the amino acid sequence of a natural variable heavy chain III (“VH3”) Ig-Fab binding region (“binding region”) of SpA, wherein the polypeptide exhibits a different binding specificity...

20060205020 - Cell-cycle checkpoint genes: This invention relates to a class of checkpoint genes and their polypeptide products which control progression through the cell cycle in eukaryotic cells. In particular, this invention relates to Schizosaccharomyces pombe rad3 gene, to its human homologue (ATR), and to their encoded proteins. The invention further relates to assay methods...

20060205021 - Mammalian iap gene family, primers, probes and detection methods: Disclosed is substantially pure DNA encoding mammalian IAP polypeptides; substantially pure polypeptides; and methods of using such DNA to express the IAP polypeptides in cells and animals to inhibit apoptosis. Also disclosed are conserved regions characteristic of the IAP family and primers and probes for the identification and isolation of...

20060205022 - Method for diagnosing and prognosing inflammatory bowel disease and crohn's disease: Disclosed are methods for diagnosing and prognosing Inflammatory Bowel disease or Crohn's disease (CD) by measuring levels of antibodies to glycans in a biological sample....

20060205024 - Method to diagnose and evaluate progression of alzheimer's disease: A method or technique of detecting, diagnosing, monitoring, and evaluating a neurodegenerative disease, such as Alzheimer's disease, in a human body fluid is disclosed. The invention provides for a method of detecting amyloid peptides associated with erythrocytes and amyloid peptide complement complexes, as a diagnostic test for the detection, monitoring,...

20060205025 - Rapid prion-detection assay: Assays are provided for rapid detection, with high specificity of the pathogenic form of prion protein responsible for neurodegenerative diseases affecting humans and animals, such as transmissible spongiform encephalopathy in bovine, sheep, and cats. Also provided are assays for testing animal feedstock, such as animal feed, for the presence or...

20060205023 - Sp1 as a marker in diagnosis and prognosis of non-alcoholic steatohepatitis (nash) and target in drug screening for nash: Phosphorylated Sp1 can be used as a marker for in vitro diagnosis of non-alcoholic steatohepatitis (NASH) as well as a therapeutical target of said condition....

20060205027 - Alkylguanyltransferase assays: This invention provides a method to determine alkylguanyltransferase activity in a sample, comprising steps of placing the sample in an appropriate condition so that the AGT is functional; contacting the sample with an AGT Detector under conditions permitting the binding of AGT and AGTD to produce a signal; and measuring...

20060205026 - Parallel inducible cell-based kinase screen: A parallel inducible cell-based kinase screen (PICKS) includes a plurality of cells that express a kinase gene under the control of an inducer and a reporter gene, wherein the cells are derived from a single cell line, and wherein the reporter gene generate a signal in response to catalytic activity...

20060205028 - Active-site titration of glycosyl hydrolases: The invention provides a method of screening for a property of a glycosyl hydrolase wherein the property is dependent on the concentration of the glycosyl hydrolase. The method comprises a step for determining the concentration of the glycosyl hydrolase by active site titration....

20060205029 - Device for the determination of glycated hemoglobin: A method of determining the percentage of glycated hemoglobin in a blood sample is disclosed wherein at least one of the assay steps is performed electrochemically. The method includes determining the total amount of hemoglobin in a sample by electrochemically measuring, in an oxygen electroreduction reaction at a cathode, the...

20060205030 - Dna for encoding d-hydantoin hydrolases, dna for encoding n-carbamyl-d-amino acid hydrolases, recombinant dna containing the genes, cells transformed with the recombinant dna, methods for producing proteins utilizing the transformed cells and methods for: DNA for encoding a protein having D-hydantoinase activity which has a base sequence represented by Sequence ID No. 1 in the Sequence Listing. DNA for encoding a protein having D-carbamylase activity which has a base sequence represented by Sequence ID No. 3 in the Sequence Listing....

20060205035 - Biologic variability of asthma associated factors useful in treating and diagnosing atopic allergies including asthma and related disorders: This invention relates to the diagnosis, treatment and methods for discovery of new therapeutics for atopic asthma and related disorders based on variants of Asthma Associated Factor 2. One embodiment of the invention is a variant of AAF2 wherein codon 173 is deleted resulting in the loss of glutamine 173...

20060205032 - Hslim modulates immune responses: Human SLIM polypeptides for use in modulating STAT-dependent gene transcription....

20060205033 - Infectious clones of rna viruses and vaccines and diagnostic assays derived thereof: An infectious clone based on the genome of a wild-type RNA virus is produced by the process of providing a host cell not susceptible to infection by the wild-type RNA virus, providing a recombinant nucleic acid based on the genome of the wild-type RNA virus, transfecting the host cell with...

20060205031 - Newly identified cholinephosphotransferases and ethanolaminephosphotransferases: The present invention relates to polypeptides comprising one or more of the amino acid motifs selected from the group consisting of a sequence with at least 80% identity to any of (a) P-L-X-D-X(35,75)-R-R-X(8)-[YF]-X(2)-R-X(6)-T (b) C-X-D-X(3)-S-G-H-T (c) H-Y-[TS]-X-D-[VI]-X(3)-[FYI]-X(6)-F-X(2)-Y-H, which transfer phosphocholine and phosphoethanolamine, nucleotide sequences coding for any of these polypeptides...

20060205034 - Novel genes encoding proteins having prognostic, diagnostic, preventive, therapeutic, and other uses: The invention provides isolated nucleic acid molecules and polypeptide molecules. The invention also provides antisense nucleic acid molecules, expression vectors containing the nucleic acid molecules of the invention, host cells into which the expression vectors have been introduced, and non-human transgenic animals in which a nucleic acid molecule of the...

20060205038 - Il-i7 homologous polypeptides and therapeutic uses thereof: The present invention is directed to novel polypeptides having sequence identity with IL-17 and to nucleic acid molecules encoding those polypeptides. Also provided herein are vectors and host cells comprising those nucleic acid sequences, chimeric polypeptide molecules comprising the polypeptides of the present invention fused to heterologous polypeptide sequences, antibodies...

20060205036 - Coagulation factor vii polypeptides: The present invention relates to novel coagulation Factor VII polypeptides, polynucleotide constructs encoding such polypeptides, as well as vectors and host cells comprising and expressing the polynucleotide, pharmaceutical compositions, uses and methods of treatment....

20060205037 - Modified transferrin fusion proteins: Modified fusion proteins of a transferrini moiety, a GLP-1 moiety and a linker moiety, with increased productivity, bioactivity and serum half-life are disclosed. Preferred fusion proteins include those modified so that the transferrin moiety exhibits no or reduced glycosylation. The fusion proteins of the invention are useful for the treatment...

20060205039 - Process for producing sugar chain asparagine derivative: A process for preparing asparagine-linked oligosaccharide derivatives including the steps of: (a) treating a delipidated egg yolk with a protease to obtain a mixture of peptide-linked oligosaccharides, (b) treating the mixture of peptide-linked oligosaccharides with a peptidase to obtain a mixture of asparagine-linked oligosaccharides, (c) introducing a lipophilic protective group...

20060205040 - Compositions for use in identification of adventitious viruses: The present invention provides compositions, kits and methods for rapid identification and quantification of adventitious contaminant viruses by molecular mass and base composition analysis....

20060205041 - Recombinant bovine thrombin: Methods are disclosed for producing recombinant thrombin from precursor molecules that do not require activation by Factor Xa. The protein is produced from host cells transformed or transfected with DNA construct(s) containing information necessary to direct the expression of thrombin precursors. Methods for purification of the thrombin precursors are also...

20060205042 - Novel variant hypocrea jecorina cbh2 cellulases: Described herein are variants of H. jecorina CBH2, a Cel6A enzyme. The present invention provides novel cellobiohydrolases that have altered thermostability....

20060205044 - Escherichia coli strains that over-produce l-threonine and processes for their production: The invention relates to novel bacterial strains and constructs as well as methods for production of L-amino acids, including but not limited to L-threonine. Such novel bacterial strains may be characterized by, for instance, Escherichia coli strains in which an aspartate semialdehyde dehydrogenase (asd) gene is operably associated with at...

20060205043 - Method for producing target substance by fermentation: A method for producing a target substance by utilizing a microorganism by culturing the microorganism in a medium to produce and accumulate the target substance in the medium and collecting the target substance from the culture is described. The microorganism is a mutant recombinant strain in which maltose assimilation is...

20060205045 - Novel dehydrogenase and a gene encodign the same: The object of the present invention is to provide a novel dehydrogenase having a property which is different from that of known dehydrogenases. The present invention provides a dehydrogenase having the following physicochemical properties: (1) effect: to produce N-alkyl-L-alanine from pyruvic acid and alkylamine or dialkylamine using NADPH and/or NADH...

20060205046 - Nucleotide sequences which code for the zwa1 gene: Disclosed herein is an isolated polynucleotide comprising a polynucleotide sequence chosen from the group consisting of a) polynucleotide which is identical to the extent of at least 70% to a polynucleotide which codes for a polypeptide which comprises the amino acid sequence of SEQ ID NO:2, b) polynucleotide which codes...

20060205047 - Method for producing gamma-linolenic acids from a ciliate culture by adding suitable precursor molecules to said culture medium: The invention relates to a method for the fermentative production of lipids/fatty acids having high concentrations of omega-6 fatty acids (PUFA, polyunsaturated fatty acids), especially γ-linolenic acids (GLA) in ciliates. Said lipids/fatty acids can be used in human/animal food, and in the pharmaceutical field....

20060205048 - Process for producing succinic acid: Succinic acid is produced by allowing a bacterium modified to enhance fumarate reductase activity or cell preparation thereof to react with an organic raw material in a reaction solution containing one of a carbonate ion, a bicarbonate ion, and carbon dioxide gas to generate succinic acid. More preferably, succinic acid...

20060205049 - Method for the production of resveratrol in cell cultures: The invention relates to a method for the production of resveratrol in cell cultures. The inventive method consists in: incubating a culture of cells which produce resveratrol naturally, in suspension, in the presence of randomly-methylated β-cyclodextrin (RMBCD) with a degree of substitution of between 11 and 13 under conditions that...

20060205050 - Plant mangrove-associated fungus culvularia lunata and a simple and efficient method of obtaining high yield of pure mannitol from the same: The present invention relates to a plant mangrove-associated fungus Culvularia lunata, and a simple and efficient method of obtaining high yield of pure mannitol from a plant mangrove-associated fungus Curvularia lunata, said method comprising steps of cutting the leaves into small pieces and placing it on potato dextrose agar (PDA)...

20060205051 - Process for anaerobic oxidation of methane: Methane can be used as a source for producing hydrogen or hydrogen equivalents in a biological process using mesophilic or thermophilic bacteria of the order of the Thermotogales, e.g. Thermotoga maritima. Hydrogen can be produced as such, or as a reducing equivalent for the reduction of various compounds such as...

20060205052 - Bardet-biedl susceptibility gene and uses thereof: The present invention relates to the identification of a gene, now designated negevin (ngvn), that is involved in the genetic disease Bardet Biedl Syndrome (BBS), which is characterized by such diverse symptoms as obesity, diabetes, hypertension, mental retardation, renal cancer and other abnormalities, retinopathy and hypogonadism. The human NGVN protein...

20060205053 - 27411, a novel human pgp synthase: The present invention relates to a newly identified human PGP synthase. The invention also relates to polynucleotides encoding the PGP synthase. The invention further relates to methods using the PGP synthase polypeptides and polynucleotides as a target for diagnosis and treatment in PGP synthase-mediated or -related disorders. The invention further...

20060205054 - Extracellular serine protease: The present invention provides a DNA encoding a novel extracellular serine protease termed Tumor Antigen Derived Gene-14 (TADG-14) which is overexpressed in ovarian, breast and colon carcinoma samples. Also provided are vector and host cells capable of expressing the DNA of the present invention, as well as the uses of...

20060205055 - Method and equipment for cultivating anaerobic ammonium-oxidizing bacteria: The present invention can feed substrates without waste to generate seed sludge with high bacterial cell concentrations and can start up operation within a short time, in cultivating anaerobic ammonium oxidizing bacteria with ammonium and nitrite as substrates. Equipment for cultivating anaerobic ammonium-oxidizing bacteria, which cultivates, in a cultivation tank,...

20060205056 - Enzymatic method of making ethyl 3-hydroxy-3-phenylpropionate and their esters: The present invention provides a new process for the preparation of optically active ethyl 3-hydroxy-3-phenylpropionate and their esters by enzymatic method. More particularly, the racemic ethyl 3-hydroxy-3-phenylpropionate is converted to optically active ethyl 3-hydroxy-3-phenylpropionate and their esters by enzymatic reaction with acylating agent. This invention may be employed for the...

20060205057 - Microfluidic microarray with high surface area active regions: Described herein is a method of providing three dimensional structures in a fluidic channel. The assay system comprises a channel, for example, a microfluidic channel, and a plurality of posts mounted to a support and arranged for insertion into the channel. The posts may be treated such that the posts...

20060205058 - Spatially scanned optical reader system and method for using same: An optical reader system is described herein that uses a scanned optical beam to interrogate a biosensor to determine if a biomolecular binding event occurred on a surface of the biosensor. In one embodiment, the optical reader system includes a light source, a detector and a processor (e.g., computer, DSP)....

20060205060 - Biosensor, biosensor system and method of using thereof: A biosensor for detecting an analyte by using a variable voltage according to infrared radiation absorption is provided. The biosensor includes an infrared absorber having a variable resistance and bioreceptors immobilized onto the infrared absorber for selectively reacting and binding with an analyte, wherein an induced voltage at the infrared...

20060205061 - Biosensors based upon actuated desorption: Provided are methods and systems for a rational concatenation of specific classes of proven microfluidic-based protocols to enable a systems approach to repetitive, synchronous analyte detection via a pristine detection array under programmed and/or automatic control....

20060205059 - Dual path immunoassay device: The systems of the invention include test cells with a first sorbent material defining a first flow path for a solution, a second sorbent material defining a second flow path distinct from the first flow path for a sample, and a test line or test site with immobilized antigens or...

20060205062 - Microfluidic analysis system: A microfluidic analysis system (1) performs polymerase chain reaction (PCR) analysis on a bio sample. In a centrifuge (6) the sample is separated into DNA and RNA constituents. The vortex is created by opposing flow of a silicon oil primary carrier fluid effecting circulation by viscous drag. The bio sample...

20060205063 - Floor system for composting: A composting floor system is disclosed for accelerated and uniform decomposition of organic matter, the composting floor system including a gas delivery system, wherein the floor system facilitates drainage and collection of leachate generated during the composting process, and a collection of leachate and an efficiency and speed of aerobic...

20060205064 - Reaction vessel, reaction apparatus and reaction solution temperature control method: It is an object of the present invention to provide a reaction vessel which makes it possible to control the temperature of the reaction solution accommodated in the reaction chamber with a quick response, without any need for centrifuging when the reaction solution is accommodated in the reaction chamber, and...

20060205065 - Cell cultivating flask: A stackable flask for the culturing of cells is disclosed. The cell culture chamber is defined by a top plate and a rigid bottom tray of substantially rectangular shape connected by side and end walls, the body of the flask has imparted therein an opening connected to an angled neck,...

20060205066 - One touch-type transport medium vessel: The present invention relates to an one touch-type specimen transport culture medium vessel, including a rod assembly member including a pole type rod having a sterilized collection unit and a rod head; a grip unit; a grip cap; a transparent tube and a culture medium, wherein an adapter is selectively...

20060205067 - Compositions and methods for enhancing the immunogenicity of antigens: The present invention includes compositions, methods and kits for enhancing the immunogenicity of an antigen via fusion to a Listerial protein. The present invention further encompasses Listeria vaccine strains for enhancing the immunogenicity of an antigen....

20060205068 - Novel bt toxin receptors from lepidopteran insects and methods of use: The invention relates to Bt toxin resistance management. The invention particularly relates to the isolation and characterization of nucleic acid and polypeptides for a novel Bt toxin receptor. The nucleic acid and polypeptides are useful in identifying and designing novel Bt toxin receptor ligands including novel insecticidal toxins....

20060205069 - Compositions comprising a first agent which provides a primary activation signal to t cells and a second agent which stimulates an accessory molecule on the surface of t cells: Compositions for inducing a population of T cells to proliferate in vitro by activating the population of T cells and stimulating an accessory molecule on the surface of the T cells with a ligand which binds the accessory molecule are described. T cell proliferation occurs in the absence of exogenous...

20060205070 - Hiv tev compositions and methods of use: The disclosure encompasses p28TEV polypeptide, polynucleotides, variants, and antagonists. p28TEV polypeptides and/or polynucleotides are useful in immunogenic compositions. p28TEV polypeptide antagonists include antagonist antibodies, antisense molecules or siRNA molecules. The antagonists and composition of the disclosure can be administered alone or in combination with other agents useful in the treatment...

20060205073 - Aspirating oocytes from transplanted ovarian tissue: A method for aspirating an oocyte from a human female is provided. The method includes the step of providing at least one ovarian cortical piece, implanting the ovarian cortical piece in the human female at a heterotopic location, and triggering oocyte maturity in the at least one ovarian cortical piece....

20060205072 - Enriched pancreatic stem cell and progenitor cell populations, and methods for identifying, isolating and enriching for such populations: Enriched pancreatic stem and progenitor cell populations, and methods for identifying, isolating and enriching for pancreatic stem cells using reagents that bind to cell surface markers are provided....

20060205071 - Methods for ex-vivo expanding stem/progenitor cells: Methods of ex-vivo expansion of fetal and/or adult progenitor, and umbilical cord blood, bone marrow or peripheral blood derived stem cells in bioreactors for bone marrow transplantation, transfusion medicine, regenerative medicine and gene therapy....

20060205074 - Neural progenitor cells derived from whole bone marrow: A method is described for generating a clinically significant volume of neural progenitor cells from whole bone marrow. A mass of bone marrow cells may be grown in a culture supplemented with fibroblast growth factor-2 (FGF-2) and epidermal growth factor (EGF). Further methods of the present invention are directed to...

20060205075 - In vitro differentiation and maturation of mouse embryonic stem cells into hepatocytes: The present invention provides a method for preparing a mature hepatocyte from an embryonic stem cell in vitro, comprising: (a) culturing the embryonic stem cell so as to differentiate into an endodermal cell; (b) isolating the endodermal cell from a population of the differenciated cell; and (c) culturing the isolated...

20060205076 - Osteochondral core centrifugation system and method of osteochondral repair: An osteochondral centrifugation system for providing an osteochondral centrifuged core from a graft such as femoral condyle, for example. The centrifugation system may be used either in the operating room or, alternatively, at a tissue banking facility at the time of processing. The osteochondral centrifuged core is formed by (i)...

20060205078 - Induction of rna interference by aberrant rna: A method is provided for the induction of aberrant RNA interference (abRNAi) by introducing ‘aberrant’ RNAs into the cell wherein a long-stranded antisense or sense RNA is simultaneously introduced with a short 4-12 mer homologous or complementary or random RNA wherein the long-short RNAs induce sequence specific inhibition of the...

20060205077 - Targeted transgenesis using the rosa26 locus: The invention provides a method for targeted transgenesis using the Rosa26 locus. Suitable nucleotide acid sequences and vectors for the targeted transgenesis and recombinase mediated transgenesis are provided. The Rosa26 locus proved to be a suitable integration site allowing strong and predictable expression of inserted transgenes carrying exogenous promoters....

20060205079 - Amplifiable adeno-associated virus (aav) packaging cassettes for the production of recombinant aav vectors: High-efficiency AAV packaging constructs and methods for their use are provided in the present invention. These high-efficiency packaging constructs comprise an activating element (such as the P1 sequence located within the AAV S1 integration site of human chromosome 19) amplifiably linked to one or more AAV packaging genes. The constructs...

20060205080 - Formulations for therapeutic viruses having enhanced storage stability: Therapeutic viral formulations having enhanced storage stability are described. The formulations comprise a viral vector in addition to one or more of an aqueous cosolvent, a reversible viral-encoded protease inhibitor and a mild reducing agent or other agent that prevents specific degradation of viral components....

20060205081 - Use of methyltransferase inhibitors to enhance transgene expression: The present invention concerns enhancing transgene expression in electroporated cells. Electroporation-mediated transfection of cells has several advantages over other transfection methods. However, the efficiency of transgene expression in electroporated cells may be lower than desired for certain applications. The ability to further enhance transgene expression in electroporated cells would be...

20060199171 - Assays for tert promoter modulatory agents: Methods and compositions for assaying an agent for TERT promoter modulatory activity are provided. In the subject methods, an agent is contacted with a normal cell under assay conditions that provide for a detectable phenotype, e.g., cell death, upon modulation of TERT promoter transcription control activity. Also provided are compositions,...

20060199168 - Cell recovery: The present invention relates to apparatus for recovering selected particles having predetermined properties from a number of particles. The apparatus includes a stimulation system, a detection system, a retriever and a control system. In use, the control system is adapted to activate the stimulation system to attempt to stimulate one...

20060199173 - Device and method for characterizing spheroids: The present invention relates to a device and a method for characterizing spheroids. The to-be-characterized spheroid is introduced into a tube of an electrically insulating material and with an inner diameter which is smaller than the diameter of the to-be-characterized spheroid in such a manner that the latter is in...

20060199170 - Ferromagnetic cell and tissue culture microcarriers: A porous, collagen coated, ferromagnetic cell culture microcarrier, which is suitable for in vitro cell and tissue culture and which facilitates 3D multicellular construct generation. Also provided is a method for creating batches of microcarriers which have inserted within them magnetite (Fe3O4) in the presence of collagen, thus creating a...

20060199172 - Laminated cell culture containing hepatocytes and method for detecting endocrine-like substance: A laminated cell culture obtained by lamination of a cell layer containing hepatocytes and a cell layer containing one or more kinds of cells selected from the group consisting of vascular endothelial cells, vascular smooth muscle cells, adipocytes, hemocytes, and macrophages; a laminated cell culture system obtained by lamination of...

20060199169 - Tissue punch and tissue sample labeling methods and devices for microarray preparation, archiving and documentation: A workstation that provides an efficient method to collect biological tissues in a column tissue array format from blocks of embedded, frozen tissues, or fresh tissues. The workstation has a control unit for directing operations of the workstation and the operation unit for performing the production of the tissue column...

20060199176 - Coronavirus s peptides: Isolated polypeptides containing SEQ ID NO: 3 and functional equivalents thereof. Also disclosed are isolated nucleic acids encoding the polypeptides, related expression vectors, related host cells, related antibodies, and related compositions. Methods of producing the polypeptide, diagnosing infection with a coronavirus, and identifying a test compound for treating infection with...

20060199174 - Methods and compositions for identifying anti-hcv agents: The invention provides methods and compositions for identifying agents for treating infection by viruses that encode a nucleotide-binding NS4B protein, or functional equivalent thereof, e.g., hepatitis C virus (HCV) or other members of the family Flaviviridae. In general, the methods involve contacting an NS4B nucleotide binding motif (NBM)-containing polypeptide with...

20060199175 - Novel field testing method and device for detection of equine protozoal myeloencephalitis: The present invention discloses a quick, simple, qualitative and semi-quantitative immunologic method and device in a field test kit format for the detection and the stage of infection of certain protozoal, viral, bacterial, or nematodal pathogens in body fluids, using vaccines specific to such pathogens as antigens. The method comprises...

20060199177 - T helper cell epitopes: The present invention provides T helper cell epitopes and compositions for use in inducing an immune response comprising at least one of these epitopes. The epitopes are contained within a peptide sequence selected from the group consisting of EPINQALTLMTKNVKPL (SEQ ID NO: 12); FAGVVLAGVALGVATAA (SEQ ID NO: 13); NLNAQAIQSLRTSLEQS (SEQ...

20060199217 - Assay for human dna for gender determination: A method for determining gender from a human DNA sample. The loci of Alu element insertion is selected, amplified and evaluated in terms of size of the fragment. The gender assay utilizes AluSTXa for the X chromosome, AluSTYa for the Y chromosome, or both AluSTXa and AluSTYa, to reduce the...

20060199181 - Compositions and methods for the treatment of immune related diseases: Compositions and methods for improving detection sensitivity in nucleic acid microarray analysis are disclosed, including methods of purifying nucleic acids, methods of synthesizing fluorescent DNA probes, methods of hybridization, and methods of activating a substrate for target molecule attachment are disclosed....

20060199196 - Compositions and methods for using radio frequency identifiers in biological sciences: Provided herein are biological research methods, kits, and products that utilize radio frequency identifier technology....

20060199180 - Compositions and methods relating to ovarian specific genes and proteins: The present invention relates to newly identified nucleic acid molecules and polypeptides present in normal and neoplastic ovarian cells, including fragments, variants and derivatives of the nucleic acids and polypeptides. The present invention also relates to antibodies to the polypeptides of the invention, as well as agonists and antagonists of...

20060199190 - Detection and typing of bacterial strains: Methods for the detection and typing of bacterial strains from food products and dietary supplements, environmental samples, in vivo/in vitro samples, and for studying the natural diversity of the species are disclosed. Potential applications also include product development and/or detection and differentiation of new bacterial strains....

20060199202 - Detection of allelic expression imbalance: The present invention provides compositions and methods for the detection and characterization of allelic expression imbalance from a heterozygous gene locus. More particularly, the present invention provides compositions, kits, and methods for the determination of allelic expression imbalance by the comparison of expression levels from each of two alleles of...

20060199200 - Detection of meca-containing staphylococcus spp.: The invention provides methods to detect mecA-containing Staphylococcus spp. in biological samples using real-time PCR. Primers and probes for the detection of S. aureus are provided by the invention. Articles of manufacture containing such primers and probes for detecting mecA-containing Staphylococcus spp. are further provided by the invention....

20060199212 - Device and method for high-throughput quantification of mrna from whole blood: Disclosed are a method, device kit, and automated system for simple, reproducible, and high-throughput quantification of mRNA from whole blood. More particularly, the method, device, kit and automated system involve combinations of leukocyte filters attached to oligo(dT)-immobilized multi-well plates....

20060199213 - Diagnostic histopathology using multiplex gene expression fish: The present invention relates to a method for detecting one or more nascent RNAs in a tissue sample using FISH. In the method, a plurality of probes (8 to 82) may be used to detect a single species of nascent RNA. Further, a plurality of nascent RNA species may be...

20060199209 - Enhanced detection of biological and bioactive components by resonance raman spectroscopy: A method for the detection of compounds, including biological macromolecules and cells, using changes in Raman spectroscopic properties upon binding of at least one analyte binding partner. The method comprises contacting at least one analyte with at least one analyte binding partner, binding at least one analyte to at least...

20060199187 - Enzyme amplified electrochemical dna detection: An oligonucleotide probe for DNA detection is disclosed. The oligonucleotide probe includes a site-specific sequence. Either a co-factor or a mediator is conjugated to the site-specific sequence. The co-factor is adapted to produce an enzymatic signal that is electrochemically detectable. The mediator is adapted to enhance an enzymatic signal that...

20060199203 - Extraction of high-purity dna and rna: The present invention is directed to a method of extracting nucleic acids from samples containing prokaryotic cells and/or eukaryotic cells by (a) lysing the cells under conditions that minimize RNA expression or enzymatic degradation of RNA; (b) dispersing and diluting the lysed cells; (c) mixing the dispersed and diluted lysed...

20060199211 - Fluorous-based microarrays: A novel means of arraying fluorous tagged probes is disclosed. The method involves fluorous-tagging probes and attaching the same to a substrate having a fluorous surface. Target molecules are then identified and analyzed following attachment to the microarray....

20060199204 - Genetic testing for male factor infertility: RNA in sperm can be used as a diagnostic to distinguish between normal and affected individuals. A list of specific diagnostic transcripts is provided which is compared with transcripts obtained from the sperm of a subject. Correlation between the two transcripts is used to identify normal sperm or affected sperm....

20060199214 - Incorporation of modified nucleotides by archaeon dna polymerases and related methods: The present invention is directed toward improving the efficiency of chain terminator incorporation by Family B archaeon DNA polymerases. Previously, the low efficiency of ddNTP, and more especially dye-labeled ddNTP, incorporation has limited the usefulness of this group of DNA polymerases in protocols requiring chain terminator incorporation....

20060199197 - Isolation of rna, dna and proteins from formalin-fixed paraffin-embedded tissue specimens: Methods are disclosed for rapid, reliable and simple isolation of RNA, DNA and proteins from formalin-fixed paraffin-embedded tissue samples. RNA purified in this manner can be used to monitor gene expression levels. The tissue sample can be a tumor or other pathological tissue....

20060199189 - Low-dose, sequenced, individualized chemotherapy dosing method: A low-dose, sequenced, individualized chemotherapy dosing method wherein an identification is made of biomorphomolecular markers associated with a patient tumor to be treated, and a percentage of tumor cells expressing each of the biomorphomolecular markers is determined. A set of drugs is selected that are adapted to target the biomorphomolecular...

20060199179 - Method for diagnosis of colorectal tumors: The invention provides objective methods for detecting and diagnosing colorectal cancers and pre-malignant lesions. For example, the methods disclosed herein can reliably detect very early-stage colorectal cancers. In one embodiment, the diagnostic method involves the scoring of gene expression profiles that discriminate between adenomas and carcinomas. The profile score calculated...

20060199206 - Method for identifying skin care composition-resistant skin-binding peptides: A method for identifying skin care composition-resistant skin-binding peptides is described. The skin care composition-resistant skin-binding peptides bind strongly to skin from a skin care composition matrix and are stable therein. Peptide-based skin benefit agents, such as skin conditioners and skin colorants, based on the skin care composition-resistant skin binding...

20060199199 - Method of removing nucleic acid amplification inhibitor from biological sample and pcr system: Provided is a method of removing a nucleic acid amplification inhibitor from a biological sample. The method includes contacting the biological sample to a carboxyl group-coated solid support. Provided is also a micro-PCR system including a sample pretreatment chamber including a carboxyl group-coated solid support; a PCR chamber; and a...

20060199178 - Method of typing gene polymorphisms: Accurate and highly reproducible means of detecting a base substitution mutation (for example, SNP), an insertion mutation or a deletion mutation with the use of a nucleic acid sample in a trace amount and a method of typing gene polymorphisms using the means....

20060199191 - Methods and compositions for the synthesis of rna and dna: Methods for the production of duplexes and single-stranded RNA and/or DNA of a desired length and sequence based on a novel template design which incorporates 2 polymerase promoters, primers, and production sequences within a single molecule are provided. This single molecule template design allows high-efficiency, high-yield production of single or...

20060199210 - Methods and kits for monitoring barrett's metaplasia: Disclosed are methods and kits for assessing risk of progression of Barrett's esophagus to adenocarcinoma....

20060199194 - Methods of detection using immuno-q-amp technology: The present invention describes, in certain embodiments, a composition for detecting a tau protein comprising a modified detector molecule having two ends, a first end capable of binding the tau protein and a second end comprising a single-stranded DNA template, wherein the template is capable of being replicated by an...

20060199188 - Methods, compositions and kits for use in polynucleotide amplification: In some embodiments, the present disclosure provides admixtures and methods for PCR amplification of a target polynucleotide in the presence of forward and reverse amplification primers and in which the occurrence of primer-dimers is decreased by including a dummy target polynucleotide in the PCR reaction mix. The dummy target polynucleotide...

20060199208 - Microarray device with optimized indicator polynucleotides: Optimization techniques for selecting indicator polynucleotides for an experiment and for determining expression levels resulting from the experiment. The optimization technique corrects for variations in polynucleotide melting temperatures during analysis of the experimental results. The optimization technique selects set of indicator polynucleotides for the experiment. The optimization technique then performs...

20060199218 - Modulators of leukocyte activation, compositions and methods of use: The present invention provides compositions and methods for modulating leukocyte activation. Nucleic acids encoding proteins and proteins so encoded which are capable of modulating leukocyte activation are provided. Compositions and methods for the treatment of disorders related to leukocyte dysfunction or dysregulation are also provided. Prophylactics and methods for the...

20060199182 - Molecular identification of bacteria of genus streptococcus and related genuses: The invention concerns a method for detecting by molecular identification a bacterium of one of the species of genus Streptococcus and four related genera Enterococcus, Gemella, Abiotrophia and Granulicatella which consists of using as probe or primer: the rpoB gene or gene fragment of one said bacterium of sequences SEQ...

20060199195 - Nucleic acid encoding schwannomin-binding-proteins and products related thereto: In accordance with the present invention, there are provided novel Schwannomin-Binding-Proteins (SBPs). Nucleic acid sequences encoding such proteins and assays employing same are also disclosed. The invention SBPs can be employed in a variety of ways, for example, for the production of anti-SBP antibodies thereto, in therapeutic compositions and methods...

20060199186 - Optical monitoring of cleaving enzyme activity: A marker molecule for monitoring cleaving enzyme activity is disclosed. The marker molecule includes a protein, a peptide, or an oligonucleotide. A co-factor is conjugated to the protein, the peptide, or the oligonucleotide, thereby forming a co-factor labeled protein, a co-factor labeled peptide, or a co-factor labeled oligonucleotide. The co-factor...

20060199185 - Plasmid having a function of t-vector and expression vector, and expression of the target gene using the same: The present invention relates to a plasmid (pHCE-FOREX) functioning as both a T-vector and an expression vector, which is produced by imparting a T-vector function to an HCE promoter derived from a constitutive high-level expression vector and can express a target protein in a simple and rapid manner. Also, the...

20060199198 - Polymorphic dna fragments and uses thereof: The invention provides methods and materials for generating a reference library of restriction fragments from pooled nucleic acids that contain a sequence polymorphism. Preferably, such a library is formed by digesting genomic DNA from a pool of individuals with a first and a second restriction endonuclease to form a population...

20060199183 - Probe biochips and methods for use thereof: The invention relates to fields of use of unlabelled polynucleotide probes able to form hairpins, the biochips comprising such probes and methods allowing use thereof. The present invention also concerns methods for designing such probes and biochips. More particularly, the invention concerns the use of such unlabelled probes and biochips...

20060199216 - Programmable molecular barcodes: The present disclosure concerns methods for producing and/or using molecular barcodes. In certain embodiments of the invention, the barcodes comprise polymer backbones that may contain one or more branch structures. Tags may be attached to the backbone and/or branch structures. The barcode may also comprise a probe that can bind...

20060199184 - Protein capable of deposition onto extracellular matrix: The present invention provides the following partial fragment (a) or (b) of developmentally regulated endothelial cell locus-I (Del-1) protein: (a) a protein consisting of the amino acid sequence as shown in SEQ ID NO: 6, 8, 10, 12, 18 or 24; or (b) a protein which consists of the amino...

20060199205 - Reagent sets and gene signatures for renal tubule injury: The invention discloses reagent sets and gene signatures for predicting onset of renal tubule injury in a subject. The invention also provides a necessary set of 186 genes useful for generating signatures of varying size and performance capable of predicting onset of renal tubule injury. The invention also provides methods,...

20060199207 - Self-assembly of molecules using combinatorial hybridization: Simple and convenient methods for arranging molecules of interest in a pre-determined pattern are described. The methods use combinatorial hybridization based on interactions between complementary nucleic acid sequences to arrange the molecules of interest. The resulting arrangements, kits containing the components used in the methods, and methods of using the...

20060199193 - Sensor arrays and nucleic acid sequencing applications: Embodiments of the present invention provide devices methods for sequencing DNA using arrays of reaction cavities containing sensors to monitor changes in solutions contained in the reaction cavities. Additional embodiments provide devices and methods for sequencing DNA using arrays of reaction cavities that allow for optical monitoring of solutions in...

20060199201 - Targeted gene deletions for polysaccharide slime formers: Sphingomonas strains have extracellular polysaccharide (e.g., gellan, diutan) that is firmly attached to the cell surface. This attachment may limit polysaccharide production by impairing uptake of nutrients into the cell or due to limited sites for polysaccharide biosynthesis on the cell surface. Two genes for polysaccharide biosynthesis, designated gelM and...

20060199192 - Universal linker compositions for the release or transfer of chemical agents from a polynucleotide: A universal linker structure is provided, in which a functional group and activating leaving group are placed on a tether, allowing the placement of an electrophile at the end of any nucleic acid sequence. The electrophile on the tether can react with a second nucleic acid carrying a nucleophile when...

20060199215 - Waste water inspecting agent and waste water inspecting apparatus using the same: A waste water inspecting agent is used as an additive to a specimen solution and comprises a rod-shaped body and a capturing structured element bonded to the rod-shaped body which specifically captures an object to be captured in the specimen solution....

20060199222 - Bacillus transformation, transformants and mutant libraries: The present invention provides methods for building DNA constructs in vitro, transforming such constructs into competent Bacillus strains with good efficiency, and generating populations of mutants. Also provided is a method to assemble DNA constructs in situ....

20060199223 - Chemiluminescent compounds and their use: The present invention relates to novel chemiluminescent compounds, to a method for synthesizing these compounds, to derivatives and conjugates comprising these compounds, to the use of these compounds or conjugates thereof in chemiluminescence based assays, especially in immunoassays....

20060199221 - Correction for temperature dependence assays: The present invention provides a method to calibrate an assay for variations or fluctuations in an assay parameter such as, but not limited to, temperature that affect the measured assay signal and thereby to calculate an analyte concentration with improved accuracy. The method is preferably performed in a manner such...

20060199226 - Functional bioluminescence energy resonance transfer (bret) assay to screen, identify and characterize receptor tyrosine kinase ligands: Disclosed herein are methods of evaluating whether a test compound functions as a ligand for a receptor tyrosine kinase. Also provided are methods of making pharmaceutical compositions that include agonists, antagonists, or inverse agonists of receptor tyrosine kinases. Also provides are isolated cells that include a receptor tyrosine kinase and...

20060199219 - Method for screening anti-ligand libraries for identifying anti-ligands specific for differentially and infrequently expressed ligands: The present invention relates to screening methods and, in particular, to methods of screening anti-ligands libraries for identifying anti-ligands specific for differentially and/or infrequently expressed ligands. The method comprises the steps of providing a library of anti-ligands; providing a first subtractor ligand; providing a second target ligand; determining the amount...

20060199225 - Modified chitin binding domain and uses thereof: Compositions and methods are provided for reversibly binding chitin-binding domain (CBD) to a chitin or equivalent substrate under non-denaturing conditions. CBD from either prokaryotes or eukaryotes are modified for example, by random mutation, and screened to identify mutants that achieve this change in properties. Creating a modified CBD with an...

20060199224 - Parallel identification of antibacterial targets and inhibitors: The invention relates to methods for the parallel identification of compounds that have antibacterial activity as well as the target gene for that compound. This approach allows for coupled target validation/drug discovery, elucidation of mechanism(s) of antibacterial agents as well as discovery of new antibacterial pharmacophores having the same mechanism...

20060199220 - Protein arrays and uses thereof: The Inventors herein describe methods for the production of a functional human, animal, plant or microbe protein arrays and methods to assay for interactions between the proteins on the array with molecules of interest, for example, using such arrays to determine the in vitro metabolite profile of any drug. Such...

20060199229 - Methods for identification of modulators of osgpr116 activity, and their use in the treatment of disease: This invention relates to the identification of fatty acid or lipid amides that decrease food intake in mammals, including fatty acid ethanolamides, as ligands for the G-protein coupled receptor OSGPR116, and describes the first demonstration of a specific G-protein coupled receptor that is activated by fatty acid ethanolamides that inhibit...

20060199228 - Peptides for treatment of autoimmune diseases: Peptides for use in the therapy or prevention of Type 1 Diabetes mellitus (T1DM) are those having sequences containing QPLALEGSLQK (SEQ ID NO: 9). Examples are those having a sequence selected from the group consisting essentially of GGGPGAGSLQPLALEGSLQK (SEQ ID NO: 4), GSLQPLALEGSLQKRGIV (SEQ ID NO: 5), and QPLALEGSLQKRGIVEQ (SEQ...

20060199227 - Phuman t2r receptors for acetaminophen ranitidine, strychnine and denatomium and related assays for identifying human bitter taste modulators: The present invention relates to the discovery that specific human taste receptors in the T2R taste receptor family respond to particular bitter ligands, i.e., acetaminophen, ranitidine, strychnine and denatonium. The present invention further relates to the use of these receptors in assays for identifying ligands that modulate the activation of...

20060199231 - Detection of activation of endothelial cells as surrogate marker for angiogenesis: Methods, compositions and kits are provided for assessing angiogenesis through sensitive, direct detection of activation of endothelial cells at molecular levels. In general, activation of endothelial cells is detected by measuring the levels of cellular components and their protein complexes participating in a specific angiogenesis signaling pathway in endothelial cells....

20060199233 - Mir-155 assay: The invention provides methods for diagnosing B-cell lymphoma in an animal. In particular, the invention provides methods for distinguishing an animal having diffuse large B-cell lymphoma (DLBCL) with an activated B-cell (ABC) phenotype from an animal having DLBCL with a non-activated germinal-center (GC) phenotype. The invention also provides methods for...

20060199230 - Use of prostate specific antigen to predict drug response: A method of determining the likelihood for a subject to respond to a drug, comprising: measuring at least one PSA-related value in a subject suffering from a PSA-related disorder and having been administered a drug for treating the PSA-related disorder; comparing the PSA-related value with its respective control value; and...

20060199232 - Use of protein pse3 as a marker for colorectal cancer: The present invention relates to the diagnosis of colorectal cancer. It discloses the use of protein PSE3 (proteasome activator subunit 3) in the diagnosis of colorectal cancer. It relates to a method for diagnosis of colorectal cancer from a liquid sample, derived from an individual by measuring PSE3 in said...

20060199234 - Method of diagnosing wood decay and decay diagnostic agent: The invention provides a simple method which enables precise diagnosis of wood decay at an early stage of decay and an agent used for the diagnosis method. The invention provides a method for diagnosing wood decay, wherein wood decay is judged by contacting extract of wood to be examined (test...

20060199237 - Clinical assay for monitoring of agmatine: The present invention is directed to a process for determining the level of agmatine in biological samples, especially that of human subjects. In one embodiment, the present invention comprises an assay and a method for quantitatively detecting agmatine in blood serum and other body fluids....

20060199235 - Compositions and method for accurate early pregnancy diagnosis: The invention provides improved assays for detection of pregnancy. In the assays, pregnancy associated glycoproteins are analyzed in conjunction with progesterone analysis. The techniques of the invention overcome limitations in the prior art by reducing the rate of false positive results. The assays provided by the invention can be implemented...

20060199236 - Immunoassay with extended dynamic range: The present invention provides a method of performing a competitive assay for the detection and quantification of an analyte over an extended dynamic range. This is achieved by a multi-step sample addition method whereby different concentrations of sample are added at different times during the assay that produces a dose-response...

20060199238 - Adp detection using an enzyme-coupled reaction: Methods and compositions are provided for determining ADP in the presence of ATP. These comprise including among the assay reagents at least one of the correcting components creatine phosphokinase and phosphocreatine, pyruvate kinase and phosphoenolpyruvate, peroxidase and a non-interfering peroxidase substrate, and catalase. One aspect of the method employs formation...

20060199239 - Assessment of cardiac health and thrombotic risk in a patient: The invention features methods and compositions for assessing risk, particularly immediate risk, of thrombotic events in patients with suspected or known vascular disease, and more particularly to assessing risk of thrombotic events in patients with coronary artery disease, particularly acute myocardial infarction, stroke, unstable angina, stable angina, or restenosis. Risk...

20060199240 - Biosensor and method comprising enzymes immobilized on semiconductors: The present invention provides a method and a device that utilizes functionalized semiconductor element for detecting presence and/or concentration of an agent in an assayed sample. The device of the present invention comprises: (i) a body having a surface comprising or having associated thereto semi-conducting material that can be excited...

20060199241 - Electrochemically and optically monitoring cleaving enzyme activity: A marker molecule for monitoring cleaving enzyme activity is disclosed. The marker molecule includes a protein, a peptide, or an oligonucleotide. A co-factor is conjugated to the protein, the peptide, or the oligonucleotide, thereby forming a co-factor labeled protein, a co-factor labeled peptide, or a co-factor labeled oligonucleotide. The co-factor...

20060199242 - Chemical probe compounds that become fluorescent upon reduction, and methods for their use: Chemical stain compounds containing a fluorophore and a reducible quenching unit are disclosed. The reducible quenching unit quenches the fluorophore while in its oxidized state. Upon reduction, the quenching properties of the quenching unit are diminished or eliminated. The chemical compounds can be used in a variety of applications, including...

20060199243 - Cytological stain composition and methods of use: An aqueous staining reagent is described, a composition of a stain, a metal metabisulfite, and acid in water. The stain can be a cationic stain and is preferably thionin. The metal metabisulfite is preferably sodium metabisulfite. A method of preparing the aqueous staining reagent is described. Cytological methods for imaging...

20060199244 - Production of open-chained sophorolipids: Methods for the production of open-chained sophorolipids, involving cultivating a yeast capable of producing open-chained sophorolipids in a culture medium containing fatty acid alkyl esters, and glycerol or carbohydrates (e.g., glucose) or mixtures thereof; wherein at least about 60% of the sophorolipids produced are open-chained sophorolipids....

20060199245 - Conus protein disulfide isomerase: Protein disulfide isomerase is a major component of Conus venom ducts. The invention relates to a protein disulfide isomerase (PDI) from Conus snails, a nucleic acid sequence encoding the Conus protein disulfide isomerase, and to methods for folding disulfide-rich peptides using a protein disulfide isomerase. Oxidative folding of conotoxin precursors,...

20060199250 - Method for generating a mutant protein which efficiently binds a target molecule: The present invention relates to a method for generating a mutant protein which efficiently binds a target molecule. The method of the invention employs saturation mutagenesis and random mutagenesis approaches producing one or more mutant proteins with enhanced binding efficiency for a target molecule compared to binding of a wild-type...

20060199247 - Method for identifying modulators gene expression: A method of cloning mammalian genes encoding proteins which can function in microorganisms, particularly yeast, and can modify, complement, or suppress a genetic defect associated with an identifiable phenotypic alteration or characteristic in the micro-organism. It further relates to mammalian genes cloned by the present method, as well as to...

20060199249 - Method for protein production: To provide a novel method for obtaining a protein of interest efficiently in a soluble form when the protein is produced by expressing a foreign gene in host cells such as microorganisms etc. In a method for, by culturing host cells into which a foreign gene has been introduced, producing...

20060199248 - Q3 sparc deletion mutant and uses thereof: The invention provides for SPARC polypeptides with a mutation corresponding to a deletion of the third glutamine in the mature form of the human SPARC protein, nucleic acids encoding such polypeptides, antibodies against such polypeptides, and methods of the use of such polypeptides, nucleic acids, and antibodies....

20060199246 - Zinc-regulated prokaryotic expression cassettes: The invention concerns prokaryotic expression cassettes comprising a promoter zinc-regulated by a L. lactis zitR repressor. Said cassettes can be used for expressing genes of interest in gram-positive bacteria....

20060199251 - Cleavage of fusion proteins using granzyme b protease: A method for the preparation of a polypeptide of interest in authentic form by enzymatic cleavage of fusion proteins using Granzyme B protease (EC 3.4.21.79). There is also provided fusion proteins comprising a polypeptide of interest and a fusion partner, wherein the junction region between the polypeptide of interest and...

20060199252 - Genes encoding the synthetic pathway for the production of disorazole: The present invention relates to nucleic acid sequences and proteins derivable therefrom that have been identified in Sorangium cellulosum, which proteins are catalytically active or participate in the biosynthetic pathway of disorazoles. The invention provides novel sequences which are necessary components of the disorazole biosynthetic pathway in addition to genes...

20060199253 - Recovery of cci-779 from mother liquors: The invention provides a process for recovering CCI-779 from mother liquors....

20060199254 - Carboxylic acid reductase polypeptide, nucleotide sequence encoding same and methods of use: The invention provides the nucleotide sequence and amino acid sequence for the enzyme carboxylic acid reductase isolated from bacteria. Expression cassettes, vectors, transformed cells, and variants are also provided as methods for use of recombinant biocatalytic reagents in production of synthetic, aromatic, aliphatic and alicyclic aldehydes and alcohols....

20060199255 - Reagents for improved cell-based assays: The ability to efficiently determine the state of enzyme expression in cells has long been desired as material to the diagnosis of disease. This invention relates to cytoenzymology, and more particularly to improved reagents for use in cell-based assays, especially those using fluorogenic substrates....

20060199256 - Method for producing chiral alpha-hydroxycarboxylic acids by enzymatic hydrolysis of chiral cyanohydrins: The invention relates to a method for producing chiral α-hydroxycarboxylic crystalline acids consisting in transforming cyanhydrins (R) or (S) into α-hydroxycarboxylic acids (R) or (S), respectively by enzymatic hydrolysis in the presence of Rhodococcus erythropolis NCIMB 11540....

20060199257 - Insertion sequence-free bacteria: The present invention provides a bacterium having a genome that is genetically engineered to be smaller than the genome of its native parent strain. A bacterium with a smaller genome can produce a commercial product more efficiently. The present invention also provides methods for deleting genes and other DNA sequences...

20060199258 - Method for treating liquid materials: An apparatus and method for processing a fluid, comprising a vacuum chamber having an associated heater, to heat a condensable gas therewithin, a spray nozzle to atomize a cool fluid, having a non-condensible gas dissolved therewithin, a vacuum line to maintain reduced pressure in the vacuum chamber and remove non-condensing...

20060199259 - Instrument for monitoring dna replication: An instrument for monitoring replication of DNA is provided....

20060199260 - Microbioreactor for continuous cell culture: The present invention microscale bioreactors (microfermentors) and microscale bioreactor arrays for use in culturing cells. The microfermentors include a vessel for culturing cells and means for providing oxygen to the interior of the vessel at a concentration sufficient to support cell growth, e.g., growth of bacterial cells. Depending on the...

20060199261 - Holder and method for cooling or heating samples: A holder for a microtitreplate or an array of separate test tubes comprising a first panel (1) provided with a recess (2) for receiving each well (3) of the microtitreplate or test tubes and a second panel (7). which together with the first panel (1) and three side walls (5)...

20060199262 - Apparatus and method for removing microbial contaminants from a flowing fluid: Methods and apparatuses for removing microbial contaminants from a flowing fluid in a cell culture incubator are disclosed. Some embodiments of the invention provide a cell culture incubator including a chamber, an airflow passage through which gasses circulate within the chamber, a filter configured to filter gasses that flow through...

20060199263 - Method of isolating cells from umbilical cord: The present invention relates a method for isolating four cell types from a single umbilical cord as pure cultures. These cell lines (epithelial cells, fibroblasts, smooth muscle cells and endothelial cells) can be characterised and utilised in experimental models and for therapeutic purposes. Particularly, the umbilical cells isolated herein are...

20060199264 - Generation, characterization, and isolation of neuroepithelial stem cells and lineage restricted intermediate precursor: Multipotent neuroepithelial stem cells and lineage-restricted oligodendrocyte-astrocyte precursor cells are described. The neuroepithelial stem cells are capable of self-renewal and of differentiation into neurons, astrocytes, and oligodendrocytes. The oligodendrocyte-astrocyte precursor cells are derived from neuroepithelial stem cells, are capable of self-renewal, and can differentiate into oligodendrocytes and astrocytes, but not...

20060199265 - Seeding implantable medical devices with cells: The invention is directed to apparatus and methods for seeding an implantable medical device, such as a vascular prosthesis, with cells, such as endothelial cells. The invention supports techniques for seeding a luminal surface of the device with axial centrifugation. Cells are introduced in suspension into the lumen of the...

20060199266 - Caev-based vector systems: This invention relates to caprine arthritis encephalitis virus-based vectors and vector systems that are useful in the delivery of nucleic acids to both non-dividing and dividing cells. Methods for delivering nucleic acids to both non-dividing and dividing cells using the vector systems are also disclosed....

20060199171 - Assays for tert promoter modulatory agents: Methods and compositions for assaying an agent for TERT promoter modulatory activity are provided. In the subject methods, an agent is contacted with a normal cell under assay conditions that provide for a detectable phenotype, e.g., cell death, upon modulation of TERT promoter transcription control activity. Also provided are compositions,...

20060199168 - Cell recovery: The present invention relates to apparatus for recovering selected particles having predetermined properties from a number of particles. The apparatus includes a stimulation system, a detection system, a retriever and a control system. In use, the control system is adapted to activate the stimulation system to attempt to stimulate one...

20060199173 - Device and method for characterizing spheroids: The present invention relates to a device and a method for characterizing spheroids. The to-be-characterized spheroid is introduced into a tube of an electrically insulating material and with an inner diameter which is smaller than the diameter of the to-be-characterized spheroid in such a manner that the latter is in...

20060199170 - Ferromagnetic cell and tissue culture microcarriers: A porous, collagen coated, ferromagnetic cell culture microcarrier, which is suitable for in vitro cell and tissue culture and which facilitates 3D multicellular construct generation. Also provided is a method for creating batches of microcarriers which have inserted within them magnetite (Fe3O4) in the presence of collagen, thus creating a...

20060199172 - Laminated cell culture containing hepatocytes and method for detecting endocrine-like substance: A laminated cell culture obtained by lamination of a cell layer containing hepatocytes and a cell layer containing one or more kinds of cells selected from the group consisting of vascular endothelial cells, vascular smooth muscle cells, adipocytes, hemocytes, and macrophages; a laminated cell culture system obtained by lamination of...

20060199169 - Tissue punch and tissue sample labeling methods and devices for microarray preparation, archiving and documentation: A workstation that provides an efficient method to collect biological tissues in a column tissue array format from blocks of embedded, frozen tissues, or fresh tissues. The workstation has a control unit for directing operations of the workstation and the operation unit for performing the production of the tissue column...

20060199176 - Coronavirus s peptides: Isolated polypeptides containing SEQ ID NO: 3 and functional equivalents thereof. Also disclosed are isolated nucleic acids encoding the polypeptides, related expression vectors, related host cells, related antibodies, and related compositions. Methods of producing the polypeptide, diagnosing infection with a coronavirus, and identifying a test compound for treating infection with...

20060199174 - Methods and compositions for identifying anti-hcv agents: The invention provides methods and compositions for identifying agents for treating infection by viruses that encode a nucleotide-binding NS4B protein, or functional equivalent thereof, e.g., hepatitis C virus (HCV) or other members of the family Flaviviridae. In general, the methods involve contacting an NS4B nucleotide binding motif (NBM)-containing polypeptide with...

20060199175 - Novel field testing method and device for detection of equine protozoal myeloencephalitis: The present invention discloses a quick, simple, qualitative and semi-quantitative immunologic method and device in a field test kit format for the detection and the stage of infection of certain protozoal, viral, bacterial, or nematodal pathogens in body fluids, using vaccines specific to such pathogens as antigens. The method comprises...

20060199177 - T helper cell epitopes: The present invention provides T helper cell epitopes and compositions for use in inducing an immune response comprising at least one of these epitopes. The epitopes are contained within a peptide sequence selected from the group consisting of EPINQALTLMTKNVKPL (SEQ ID NO: 12); FAGVVLAGVALGVATAA (SEQ ID NO: 13); NLNAQAIQSLRTSLEQS (SEQ...

20060199217 - Assay for human dna for gender determination: A method for determining gender from a human DNA sample. The loci of Alu element insertion is selected, amplified and evaluated in terms of size of the fragment. The gender assay utilizes AluSTXa for the X chromosome, AluSTYa for the Y chromosome, or both AluSTXa and AluSTYa, to reduce the...

20060199181 - Compositions and methods for the treatment of immune related diseases: Compositions and methods for improving detection sensitivity in nucleic acid microarray analysis are disclosed, including methods of purifying nucleic acids, methods of synthesizing fluorescent DNA probes, methods of hybridization, and methods of activating a substrate for target molecule attachment are disclosed....

20060199196 - Compositions and methods for using radio frequency identifiers in biological sciences: Provided herein are biological research methods, kits, and products that utilize radio frequency identifier technology....

20060199180 - Compositions and methods relating to ovarian specific genes and proteins: The present invention relates to newly identified nucleic acid molecules and polypeptides present in normal and neoplastic ovarian cells, including fragments, variants and derivatives of the nucleic acids and polypeptides. The present invention also relates to antibodies to the polypeptides of the invention, as well as agonists and antagonists of...

20060199190 - Detection and typing of bacterial strains: Methods for the detection and typing of bacterial strains from food products and dietary supplements, environmental samples, in vivo/in vitro samples, and for studying the natural diversity of the species are disclosed. Potential applications also include product development and/or detection and differentiation of new bacterial strains....

20060199202 - Detection of allelic expression imbalance: The present invention provides compositions and methods for the detection and characterization of allelic expression imbalance from a heterozygous gene locus. More particularly, the present invention provides compositions, kits, and methods for the determination of allelic expression imbalance by the comparison of expression levels from each of two alleles of...

20060199200 - Detection of meca-containing staphylococcus spp.: The invention provides methods to detect mecA-containing Staphylococcus spp. in biological samples using real-time PCR. Primers and probes for the detection of S. aureus are provided by the invention. Articles of manufacture containing such primers and probes for detecting mecA-containing Staphylococcus spp. are further provided by the invention....

20060199212 - Device and method for high-throughput quantification of mrna from whole blood: Disclosed are a method, device kit, and automated system for simple, reproducible, and high-throughput quantification of mRNA from whole blood. More particularly, the method, device, kit and automated system involve combinations of leukocyte filters attached to oligo(dT)-immobilized multi-well plates....

20060199213 - Diagnostic histopathology using multiplex gene expression fish: The present invention relates to a method for detecting one or more nascent RNAs in a tissue sample using FISH. In the method, a plurality of probes (8 to 82) may be used to detect a single species of nascent RNA. Further, a plurality of nascent RNA species may be...

20060199209 - Enhanced detection of biological and bioactive components by resonance raman spectroscopy: A method for the detection of compounds, including biological macromolecules and cells, using changes in Raman spectroscopic properties upon binding of at least one analyte binding partner. The method comprises contacting at least one analyte with at least one analyte binding partner, binding at least one analyte to at least...

20060199187 - Enzyme amplified electrochemical dna detection: An oligonucleotide probe for DNA detection is disclosed. The oligonucleotide probe includes a site-specific sequence. Either a co-factor or a mediator is conjugated to the site-specific sequence. The co-factor is adapted to produce an enzymatic signal that is electrochemically detectable. The mediator is adapted to enhance an enzymatic signal that...

20060199203 - Extraction of high-purity dna and rna: The present invention is directed to a method of extracting nucleic acids from samples containing prokaryotic cells and/or eukaryotic cells by (a) lysing the cells under conditions that minimize RNA expression or enzymatic degradation of RNA; (b) dispersing and diluting the lysed cells; (c) mixing the dispersed and diluted lysed...

20060199211 - Fluorous-based microarrays: A novel means of arraying fluorous tagged probes is disclosed. The method involves fluorous-tagging probes and attaching the same to a substrate having a fluorous surface. Target molecules are then identified and analyzed following attachment to the microarray....

20060199204 - Genetic testing for male factor infertility: RNA in sperm can be used as a diagnostic to distinguish between normal and affected individuals. A list of specific diagnostic transcripts is provided which is compared with transcripts obtained from the sperm of a subject. Correlation between the two transcripts is used to identify normal sperm or affected sperm....

20060199214 - Incorporation of modified nucleotides by archaeon dna polymerases and related methods: The present invention is directed toward improving the efficiency of chain terminator incorporation by Family B archaeon DNA polymerases. Previously, the low efficiency of ddNTP, and more especially dye-labeled ddNTP, incorporation has limited the usefulness of this group of DNA polymerases in protocols requiring chain terminator incorporation....

20060199197 - Isolation of rna, dna and proteins from formalin-fixed paraffin-embedded tissue specimens: Methods are disclosed for rapid, reliable and simple isolation of RNA, DNA and proteins from formalin-fixed paraffin-embedded tissue samples. RNA purified in this manner can be used to monitor gene expression levels. The tissue sample can be a tumor or other pathological tissue....

20060199189 - Low-dose, sequenced, individualized chemotherapy dosing method: A low-dose, sequenced, individualized chemotherapy dosing method wherein an identification is made of biomorphomolecular markers associated with a patient tumor to be treated, and a percentage of tumor cells expressing each of the biomorphomolecular markers is determined. A set of drugs is selected that are adapted to target the biomorphomolecular...

20060199179 - Method for diagnosis of colorectal tumors: The invention provides objective methods for detecting and diagnosing colorectal cancers and pre-malignant lesions. For example, the methods disclosed herein can reliably detect very early-stage colorectal cancers. In one embodiment, the diagnostic method involves the scoring of gene expression profiles that discriminate between adenomas and carcinomas. The profile score calculated...

20060199206 - Method for identifying skin care composition-resistant skin-binding peptides: A method for identifying skin care composition-resistant skin-binding peptides is described. The skin care composition-resistant skin-binding peptides bind strongly to skin from a skin care composition matrix and are stable therein. Peptide-based skin benefit agents, such as skin conditioners and skin colorants, based on the skin care composition-resistant skin binding...

20060199199 - Method of removing nucleic acid amplification inhibitor from biological sample and pcr system: Provided is a method of removing a nucleic acid amplification inhibitor from a biological sample. The method includes contacting the biological sample to a carboxyl group-coated solid support. Provided is also a micro-PCR system including a sample pretreatment chamber including a carboxyl group-coated solid support; a PCR chamber; and a...

20060199178 - Method of typing gene polymorphisms: Accurate and highly reproducible means of detecting a base substitution mutation (for example, SNP), an insertion mutation or a deletion mutation with the use of a nucleic acid sample in a trace amount and a method of typing gene polymorphisms using the means....

20060199191 - Methods and compositions for the synthesis of rna and dna: Methods for the production of duplexes and single-stranded RNA and/or DNA of a desired length and sequence based on a novel template design which incorporates 2 polymerase promoters, primers, and production sequences within a single molecule are provided. This single molecule template design allows high-efficiency, high-yield production of single or...

20060199210 - Methods and kits for monitoring barrett's metaplasia: Disclosed are methods and kits for assessing risk of progression of Barrett's esophagus to adenocarcinoma....

20060199194 - Methods of detection using immuno-q-amp technology: The present invention describes, in certain embodiments, a composition for detecting a tau protein comprising a modified detector molecule having two ends, a first end capable of binding the tau protein and a second end comprising a single-stranded DNA template, wherein the template is capable of being replicated by an...

20060199188 - Methods, compositions and kits for use in polynucleotide amplification: In some embodiments, the present disclosure provides admixtures and methods for PCR amplification of a target polynucleotide in the presence of forward and reverse amplification primers and in which the occurrence of primer-dimers is decreased by including a dummy target polynucleotide in the PCR reaction mix. The dummy target polynucleotide...

20060199208 - Microarray device with optimized indicator polynucleotides: Optimization techniques for selecting indicator polynucleotides for an experiment and for determining expression levels resulting from the experiment. The optimization technique corrects for variations in polynucleotide melting temperatures during analysis of the experimental results. The optimization technique selects set of indicator polynucleotides for the experiment. The optimization technique then performs...

20060199218 - Modulators of leukocyte activation, compositions and methods of use: The present invention provides compositions and methods for modulating leukocyte activation. Nucleic acids encoding proteins and proteins so encoded which are capable of modulating leukocyte activation are provided. Compositions and methods for the treatment of disorders related to leukocyte dysfunction or dysregulation are also provided. Prophylactics and methods for the...

20060199182 - Molecular identification of bacteria of genus streptococcus and related genuses: The invention concerns a method for detecting by molecular identification a bacterium of one of the species of genus Streptococcus and four related genera Enterococcus, Gemella, Abiotrophia and Granulicatella which consists of using as probe or primer: the rpoB gene or gene fragment of one said bacterium of sequences SEQ...

20060199195 - Nucleic acid encoding schwannomin-binding-proteins and products related thereto: In accordance with the present invention, there are provided novel Schwannomin-Binding-Proteins (SBPs). Nucleic acid sequences encoding such proteins and assays employing same are also disclosed. The invention SBPs can be employed in a variety of ways, for example, for the production of anti-SBP antibodies thereto, in therapeutic compositions and methods...

20060199186 - Optical monitoring of cleaving enzyme activity: A marker molecule for monitoring cleaving enzyme activity is disclosed. The marker molecule includes a protein, a peptide, or an oligonucleotide. A co-factor is conjugated to the protein, the peptide, or the oligonucleotide, thereby forming a co-factor labeled protein, a co-factor labeled peptide, or a co-factor labeled oligonucleotide. The co-factor...

20060199185 - Plasmid having a function of t-vector and expression vector, and expression of the target gene using the same: The present invention relates to a plasmid (pHCE-FOREX) functioning as both a T-vector and an expression vector, which is produced by imparting a T-vector function to an HCE promoter derived from a constitutive high-level expression vector and can express a target protein in a simple and rapid manner. Also, the...

20060199198 - Polymorphic dna fragments and uses thereof: The invention provides methods and materials for generating a reference library of restriction fragments from pooled nucleic acids that contain a sequence polymorphism. Preferably, such a library is formed by digesting genomic DNA from a pool of individuals with a first and a second restriction endonuclease to form a population...

20060199183 - Probe biochips and methods for use thereof: The invention relates to fields of use of unlabelled polynucleotide probes able to form hairpins, the biochips comprising such probes and methods allowing use thereof. The present invention also concerns methods for designing such probes and biochips. More particularly, the invention concerns the use of such unlabelled probes and biochips...

20060199216 - Programmable molecular barcodes: The present disclosure concerns methods for producing and/or using molecular barcodes. In certain embodiments of the invention, the barcodes comprise polymer backbones that may contain one or more branch structures. Tags may be attached to the backbone and/or branch structures. The barcode may also comprise a probe that can bind...

20060199184 - Protein capable of deposition onto extracellular matrix: The present invention provides the following partial fragment (a) or (b) of developmentally regulated endothelial cell locus-I (Del-1) protein: (a) a protein consisting of the amino acid sequence as shown in SEQ ID NO: 6, 8, 10, 12, 18 or 24; or (b) a protein which consists of the amino...

20060199205 - Reagent sets and gene signatures for renal tubule injury: The invention discloses reagent sets and gene signatures for predicting onset of renal tubule injury in a subject. The invention also provides a necessary set of 186 genes useful for generating signatures of varying size and performance capable of predicting onset of renal tubule injury. The invention also provides methods,...

20060199207 - Self-assembly of molecules using combinatorial hybridization: Simple and convenient methods for arranging molecules of interest in a pre-determined pattern are described. The methods use combinatorial hybridization based on interactions between complementary nucleic acid sequences to arrange the molecules of interest. The resulting arrangements, kits containing the components used in the methods, and methods of using the...

20060199193 - Sensor arrays and nucleic acid sequencing applications: Embodiments of the present invention provide devices methods for sequencing DNA using arrays of reaction cavities containing sensors to monitor changes in solutions contained in the reaction cavities. Additional embodiments provide devices and methods for sequencing DNA using arrays of reaction cavities that allow for optical monitoring of solutions in...

20060199201 - Targeted gene deletions for polysaccharide slime formers: Sphingomonas strains have extracellular polysaccharide (e.g., gellan, diutan) that is firmly attached to the cell surface. This attachment may limit polysaccharide production by impairing uptake of nutrients into the cell or due to limited sites for polysaccharide biosynthesis on the cell surface. Two genes for polysaccharide biosynthesis, designated gelM and...

20060199192 - Universal linker compositions for the release or transfer of chemical agents from a polynucleotide: A universal linker structure is provided, in which a functional group and activating leaving group are placed on a tether, allowing the placement of an electrophile at the end of any nucleic acid sequence. The electrophile on the tether can react with a second nucleic acid carrying a nucleophile when...

20060199215 - Waste water inspecting agent and waste water inspecting apparatus using the same: A waste water inspecting agent is used as an additive to a specimen solution and comprises a rod-shaped body and a capturing structured element bonded to the rod-shaped body which specifically captures an object to be captured in the specimen solution....

20060199222 - Bacillus transformation, transformants and mutant libraries: The present invention provides methods for building DNA constructs in vitro, transforming such constructs into competent Bacillus strains with good efficiency, and generating populations of mutants. Also provided is a method to assemble DNA constructs in situ....

20060199223 - Chemiluminescent compounds and their use: The present invention relates to novel chemiluminescent compounds, to a method for synthesizing these compounds, to derivatives and conjugates comprising these compounds, to the use of these compounds or conjugates thereof in chemiluminescence based assays, especially in immunoassays....

20060199221 - Correction for temperature dependence assays: The present invention provides a method to calibrate an assay for variations or fluctuations in an assay parameter such as, but not limited to, temperature that affect the measured assay signal and thereby to calculate an analyte concentration with improved accuracy. The method is preferably performed in a manner such...

20060199226 - Functional bioluminescence energy resonance transfer (bret) assay to screen, identify and characterize receptor tyrosine kinase ligands: Disclosed herein are methods of evaluating whether a test compound functions as a ligand for a receptor tyrosine kinase. Also provided are methods of making pharmaceutical compositions that include agonists, antagonists, or inverse agonists of receptor tyrosine kinases. Also provides are isolated cells that include a receptor tyrosine kinase and...

20060199219 - Method for screening anti-ligand libraries for identifying anti-ligands specific for differentially and infrequently expressed ligands: The present invention relates to screening methods and, in particular, to methods of screening anti-ligands libraries for identifying anti-ligands specific for differentially and/or infrequently expressed ligands. The method comprises the steps of providing a library of anti-ligands; providing a first subtractor ligand; providing a second target ligand; determining the amount...

20060199225 - Modified chitin binding domain and uses thereof: Compositions and methods are provided for reversibly binding chitin-binding domain (CBD) to a chitin or equivalent substrate under non-denaturing conditions. CBD from either prokaryotes or eukaryotes are modified for example, by random mutation, and screened to identify mutants that achieve this change in properties. Creating a modified CBD with an...

20060199224 - Parallel identification of antibacterial targets and inhibitors: The invention relates to methods for the parallel identification of compounds that have antibacterial activity as well as the target gene for that compound. This approach allows for coupled target validation/drug discovery, elucidation of mechanism(s) of antibacterial agents as well as discovery of new antibacterial pharmacophores having the same mechanism...

20060199220 - Protein arrays and uses thereof: The Inventors herein describe methods for the production of a functional human, animal, plant or microbe protein arrays and methods to assay for interactions between the proteins on the array with molecules of interest, for example, using such arrays to determine the in vitro metabolite profile of any drug. Such...

20060199229 - Methods for identification of modulators of osgpr116 activity, and their use in the treatment of disease: This invention relates to the identification of fatty acid or lipid amides that decrease food intake in mammals, including fatty acid ethanolamides, as ligands for the G-protein coupled receptor OSGPR116, and describes the first demonstration of a specific G-protein coupled receptor that is activated by fatty acid ethanolamides that inhibit...

20060199228 - Peptides for treatment of autoimmune diseases: Peptides for use in the therapy or prevention of Type 1 Diabetes mellitus (T1DM) are those having sequences containing QPLALEGSLQK (SEQ ID NO: 9). Examples are those having a sequence selected from the group consisting essentially of GGGPGAGSLQPLALEGSLQK (SEQ ID NO: 4), GSLQPLALEGSLQKRGIV (SEQ ID NO: 5), and QPLALEGSLQKRGIVEQ (SEQ...

20060199227 - Phuman t2r receptors for acetaminophen ranitidine, strychnine and denatomium and related assays for identifying human bitter taste modulators: The present invention relates to the discovery that specific human taste receptors in the T2R taste receptor family respond to particular bitter ligands, i.e., acetaminophen, ranitidine, strychnine and denatonium. The present invention further relates to the use of these receptors in assays for identifying ligands that modulate the activation of...

20060199231 - Detection of activation of endothelial cells as surrogate marker for angiogenesis: Methods, compositions and kits are provided for assessing angiogenesis through sensitive, direct detection of activation of endothelial cells at molecular levels. In general, activation of endothelial cells is detected by measuring the levels of cellular components and their protein complexes participating in a specific angiogenesis signaling pathway in endothelial cells....

20060199233 - Mir-155 assay: The invention provides methods for diagnosing B-cell lymphoma in an animal. In particular, the invention provides methods for distinguishing an animal having diffuse large B-cell lymphoma (DLBCL) with an activated B-cell (ABC) phenotype from an animal having DLBCL with a non-activated germinal-center (GC) phenotype. The invention also provides methods for...

20060199230 - Use of prostate specific antigen to predict drug response: A method of determining the likelihood for a subject to respond to a drug, comprising: measuring at least one PSA-related value in a subject suffering from a PSA-related disorder and having been administered a drug for treating the PSA-related disorder; comparing the PSA-related value with its respective control value; and...

20060199232 - Use of protein pse3 as a marker for colorectal cancer: The present invention relates to the diagnosis of colorectal cancer. It discloses the use of protein PSE3 (proteasome activator subunit 3) in the diagnosis of colorectal cancer. It relates to a method for diagnosis of colorectal cancer from a liquid sample, derived from an individual by measuring PSE3 in said...

20060199234 - Method of diagnosing wood decay and decay diagnostic agent: The invention provides a simple method which enables precise diagnosis of wood decay at an early stage of decay and an agent used for the diagnosis method. The invention provides a method for diagnosing wood decay, wherein wood decay is judged by contacting extract of wood to be examined (test...

20060199237 - Clinical assay for monitoring of agmatine: The present invention is directed to a process for determining the level of agmatine in biological samples, especially that of human subjects. In one embodiment, the present invention comprises an assay and a method for quantitatively detecting agmatine in blood serum and other body fluids....

20060199235 - Compositions and method for accurate early pregnancy diagnosis: The invention provides improved assays for detection of pregnancy. In the assays, pregnancy associated glycoproteins are analyzed in conjunction with progesterone analysis. The techniques of the invention overcome limitations in the prior art by reducing the rate of false positive results. The assays provided by the invention can be implemented...

20060199236 - Immunoassay with extended dynamic range: The present invention provides a method of performing a competitive assay for the detection and quantification of an analyte over an extended dynamic range. This is achieved by a multi-step sample addition method whereby different concentrations of sample are added at different times during the assay that produces a dose-response...

20060199238 - Adp detection using an enzyme-coupled reaction: Methods and compositions are provided for determining ADP in the presence of ATP. These comprise including among the assay reagents at least one of the correcting components creatine phosphokinase and phosphocreatine, pyruvate kinase and phosphoenolpyruvate, peroxidase and a non-interfering peroxidase substrate, and catalase. One aspect of the method employs formation...

20060199239 - Assessment of cardiac health and thrombotic risk in a patient: The invention features methods and compositions for assessing risk, particularly immediate risk, of thrombotic events in patients with suspected or known vascular disease, and more particularly to assessing risk of thrombotic events in patients with coronary artery disease, particularly acute myocardial infarction, stroke, unstable angina, stable angina, or restenosis. Risk...

20060199240 - Biosensor and method comprising enzymes immobilized on semiconductors: The present invention provides a method and a device that utilizes functionalized semiconductor element for detecting presence and/or concentration of an agent in an assayed sample. The device of the present invention comprises: (i) a body having a surface comprising or having associated thereto semi-conducting material that can be excited...

20060199241 - Electrochemically and optically monitoring cleaving enzyme activity: A marker molecule for monitoring cleaving enzyme activity is disclosed. The marker molecule includes a protein, a peptide, or an oligonucleotide. A co-factor is conjugated to the protein, the peptide, or the oligonucleotide, thereby forming a co-factor labeled protein, a co-factor labeled peptide, or a co-factor labeled oligonucleotide. The co-factor...

20060199242 - Chemical probe compounds that become fluorescent upon reduction, and methods for their use: Chemical stain compounds containing a fluorophore and a reducible quenching unit are disclosed. The reducible quenching unit quenches the fluorophore while in its oxidized state. Upon reduction, the quenching properties of the quenching unit are diminished or eliminated. The chemical compounds can be used in a variety of applications, including...

20060199243 - Cytological stain composition and methods of use: An aqueous staining reagent is described, a composition of a stain, a metal metabisulfite, and acid in water. The stain can be a cationic stain and is preferably thionin. The metal metabisulfite is preferably sodium metabisulfite. A method of preparing the aqueous staining reagent is described. Cytological methods for imaging...

20060199244 - Production of open-chained sophorolipids: Methods for the production of open-chained sophorolipids, involving cultivating a yeast capable of producing open-chained sophorolipids in a culture medium containing fatty acid alkyl esters, and glycerol or carbohydrates (e.g., glucose) or mixtures thereof; wherein at least about 60% of the sophorolipids produced are open-chained sophorolipids....

20060199245 - Conus protein disulfide isomerase: Protein disulfide isomerase is a major component of Conus venom ducts. The invention relates to a protein disulfide isomerase (PDI) from Conus snails, a nucleic acid sequence encoding the Conus protein disulfide isomerase, and to methods for folding disulfide-rich peptides using a protein disulfide isomerase. Oxidative folding of conotoxin precursors,...

20060199250 - Method for generating a mutant protein which efficiently binds a target molecule: The present invention relates to a method for generating a mutant protein which efficiently binds a target molecule. The method of the invention employs saturation mutagenesis and random mutagenesis approaches producing one or more mutant proteins with enhanced binding efficiency for a target molecule compared to binding of a wild-type...

20060199247 - Method for identifying modulators gene expression: A method of cloning mammalian genes encoding proteins which can function in microorganisms, particularly yeast, and can modify, complement, or suppress a genetic defect associated with an identifiable phenotypic alteration or characteristic in the micro-organism. It further relates to mammalian genes cloned by the present method, as well as to...

20060199249 - Method for protein production: To provide a novel method for obtaining a protein of interest efficiently in a soluble form when the protein is produced by expressing a foreign gene in host cells such as microorganisms etc. In a method for, by culturing host cells into which a foreign gene has been introduced, producing...

20060199248 - Q3 sparc deletion mutant and uses thereof: The invention provides for SPARC polypeptides with a mutation corresponding to a deletion of the third glutamine in the mature form of the human SPARC protein, nucleic acids encoding such polypeptides, antibodies against such polypeptides, and methods of the use of such polypeptides, nucleic acids, and antibodies....

20060199246 - Zinc-regulated prokaryotic expression cassettes: The invention concerns prokaryotic expression cassettes comprising a promoter zinc-regulated by a L. lactis zitR repressor. Said cassettes can be used for expressing genes of interest in gram-positive bacteria....

20060199251 - Cleavage of fusion proteins using granzyme b protease: A method for the preparation of a polypeptide of interest in authentic form by enzymatic cleavage of fusion proteins using Granzyme B protease (EC 3.4.21.79). There is also provided fusion proteins comprising a polypeptide of interest and a fusion partner, wherein the junction region between the polypeptide of interest and...

20060199252 - Genes encoding the synthetic pathway for the production of disorazole: The present invention relates to nucleic acid sequences and proteins derivable therefrom that have been identified in Sorangium cellulosum, which proteins are catalytically active or participate in the biosynthetic pathway of disorazoles. The invention provides novel sequences which are necessary components of the disorazole biosynthetic pathway in addition to genes...

20060199253 - Recovery of cci-779 from mother liquors: The invention provides a process for recovering CCI-779 from mother liquors....

20060199254 - Carboxylic acid reductase polypeptide, nucleotide sequence encoding same and methods of use: The invention provides the nucleotide sequence and amino acid sequence for the enzyme carboxylic acid reductase isolated from bacteria. Expression cassettes, vectors, transformed cells, and variants are also provided as methods for use of recombinant biocatalytic reagents in production of synthetic, aromatic, aliphatic and alicyclic aldehydes and alcohols....

20060199255 - Reagents for improved cell-based assays: The ability to efficiently determine the state of enzyme expression in cells has long been desired as material to the diagnosis of disease. This invention relates to cytoenzymology, and more particularly to improved reagents for use in cell-based assays, especially those using fluorogenic substrates....

20060199256 - Method for producing chiral alpha-hydroxycarboxylic acids by enzymatic hydrolysis of chiral cyanohydrins: The invention relates to a method for producing chiral α-hydroxycarboxylic crystalline acids consisting in transforming cyanhydrins (R) or (S) into α-hydroxycarboxylic acids (R) or (S), respectively by enzymatic hydrolysis in the presence of Rhodococcus erythropolis NCIMB 11540....

20060199257 - Insertion sequence-free bacteria: The present invention provides a bacterium having a genome that is genetically engineered to be smaller than the genome of its native parent strain. A bacterium with a smaller genome can produce a commercial product more efficiently. The present invention also provides methods for deleting genes and other DNA sequences...

20060199258 - Method for treating liquid materials: An apparatus and method for processing a fluid, comprising a vacuum chamber having an associated heater, to heat a condensable gas therewithin, a spray nozzle to atomize a cool fluid, having a non-condensible gas dissolved therewithin, a vacuum line to maintain reduced pressure in the vacuum chamber and remove non-condensing...

20060199259 - Instrument for monitoring dna replication: An instrument for monitoring replication of DNA is provided....

20060199260 - Microbioreactor for continuous cell culture: The present invention microscale bioreactors (microfermentors) and microscale bioreactor arrays for use in culturing cells. The microfermentors include a vessel for culturing cells and means for providing oxygen to the interior of the vessel at a concentration sufficient to support cell growth, e.g., growth of bacterial cells. Depending on the...

20060199261 - Holder and method for cooling or heating samples: A holder for a microtitreplate or an array of separate test tubes comprising a first panel (1) provided with a recess (2) for receiving each well (3) of the microtitreplate or test tubes and a second panel (7). which together with the first panel (1) and three side walls (5)...

20060199262 - Apparatus and method for removing microbial contaminants from a flowing fluid: Methods and apparatuses for removing microbial contaminants from a flowing fluid in a cell culture incubator are disclosed. Some embodiments of the invention provide a cell culture incubator including a chamber, an airflow passage through which gasses circulate within the chamber, a filter configured to filter gasses that flow through...

20060199263 - Method of isolating cells from umbilical cord: The present invention relates a method for isolating four cell types from a single umbilical cord as pure cultures. These cell lines (epithelial cells, fibroblasts, smooth muscle cells and endothelial cells) can be characterised and utilised in experimental models and for therapeutic purposes. Particularly, the umbilical cells isolated herein are...

20060199264 - Generation, characterization, and isolation of neuroepithelial stem cells and lineage restricted intermediate precursor: Multipotent neuroepithelial stem cells and lineage-restricted oligodendrocyte-astrocyte precursor cells are described. The neuroepithelial stem cells are capable of self-renewal and of differentiation into neurons, astrocytes, and oligodendrocytes. The oligodendrocyte-astrocyte precursor cells are derived from neuroepithelial stem cells, are capable of self-renewal, and can differentiate into oligodendrocytes and astrocytes, but not...

20060199265 - Seeding implantable medical devices with cells: The invention is directed to apparatus and methods for seeding an implantable medical device, such as a vascular prosthesis, with cells, such as endothelial cells. The invention supports techniques for seeding a luminal surface of the device with axial centrifugation. Cells are introduced in suspension into the lumen of the...

20060199266 - Caev-based vector systems: This invention relates to caprine arthritis encephalitis virus-based vectors and vector systems that are useful in the delivery of nucleic acids to both non-dividing and dividing cells. Methods for delivering nucleic acids to both non-dividing and dividing cells using the vector systems are also disclosed....