Chemistry: molecular biology and microbiology inventions 08/06

20060194191 - Processing method for the long-term stabilization of biological red blood cell volume: The present invention relates to a processing method for long-term stabilization of biological red blood cell volume. The red blood cells can be further used in reference materials and calibrators of a hematology analyzer. The method of the present invention comprises contacting the sample with a solution comprising a membrane-fixation...

20060194192 - Stabilization of cells and biological specimens for analysis: Compositions and methods for stabilizing rare cells in blood specimens, preserving the quality of blood specimens, and also serving as cell fixatives are disclosed which minimize losses of target cells (for example, circulating tumor cells) and formation of debris and aggregates from target cells, non-target cells and plasma components, thereby...

20060194193 - Biological sample culturing and observation system, incubator, supplying device, and culture vessel: The present invention provides a biological sample culturing and observation system which includes: an incubator in which a biological samples are housed, and whose interior is maintained in a culturing environment satisfying predetermined conditions, and the interior is isolated from the outside; an observation optical system that from outside the...

20060194194 - Diagnostic measurement of disease: Diagnostic systems and methods that can be used to perform direct tests on immune system or other proliferative cells, which are capable of identifying abnormal cell function associate with disease without use of labeled reagents; that also permit, but do not require provision of an autoantigen; that are capable of...

20060194197 - Biosensors utilizing dendrimer-immobilized ligands and their use thereof: The present invention is directed to methods and compositions useful as biosensors that specifically interact with various pathogens and other target analytes. The biosensor itself, comprises functionalized dendritic tethers derivatized for attachment to a variety of surfaces as self-assembled monolayers (SAMs) as well as attached binding moieties (sometimes referred to...

20060194195 - Multiple antigenic peptide assay for detection of hiv or siv type retroviruses: A method for detecting at least one antibody directed against at least one primate immunodeficiency virus in a biological sample that includes contacting a biological sample with (i) at least one detection multiple antigenic peptide comprising a portion of an immunodominant region of a transmembrane protein of a primate immunodeficiency...

20060194196 - Novel surface protein (hbsag) variant of the hepatitis b virus: The invention relates to sequences of a novel variant of the Hepatitis B surface antigen (HBsAg) and to methods for detecting, in patient samples, nucleic acids, antigens and antibodies directed against the same....

20060194198 - Use of fibrous protein fibers for chemical sensing and radiation detection: Fibrous protein fibers such as keratin fibers can be used to detect chemicals and radiation. One aspect of the invention is a method of detecting an analyte in a sample comprising the steps of: (1) providing a fiber of fibrous protein; (2) contacting the fiber of fibrous protein with an...

20060194201 - Autism gene: The present invention concerns genes containing mutations associated with autism its onset and development and also to the encoded proteins of said genes associated with autism, its onset and development and the use of said genes, proteins or protein isoforms. The invention thus also relates to methods of screening for,...

20060194205 - Biochip with independent recognition areas and optical format and float scanning thereof: A biochip, and a device for reading the biochip, the biochip including a plurality of molecular recognition areas distributed with a determined layout to create a format of molecular recognition areas and a mechanism for making optical position marks for each molecular recognition area, distributed with a determined layout to...

20060194250 - Cancer diagnostic method based upon dna methylation differences: There is disclosed a cancer diagnostic method based upon DNA methylation differences at specific CpG sites. Specifically, the inventive method provides for a bisulfite treatment of DNA, followed by methylation-sensitive single nucleotide primer extension (Ms-SNuPE), for determination of strand-specific methylation status at cytosine residues....

20060194229 - Cancer markers and detection methods: The present invention relates to cancer markers and methods of detecting cancer markers in a sample. The sample may be peripheral blood. Cancer markers are most commonly mutated or abnormal DNA sequences associated with metastatic cancer. Markers may be detected using PCR, microarrays, or other nucleic acid or peptide-based assays....

20060194220 - Compositions and methods for detecting and treating tumors: In certain embodiments, this present disclosure provides compositions and methods for detecting EphB4 gene amplification in test cells. In certain embodiments, the present disclosure provides methods and compositions for evaluating tumor (cancer) status and prognosis in a subject having or suspected of having a tumor....

20060194240 - Compositions and methods of detecting an analyte by using a nucleic acid hybridization switch probe: Compositions are described for detecting binding of an analyte to a binding partner attached to a nucleic acid hybridization switch probe that includes first and second arm sequences and a support sequence that is at least partially complementary to both arm sequences, allowing the probe under hybridization conditions to form...

20060194224 - Computer-aided nucleic acid sequencing: A computer system for sequencing nucleic acids is provided. The computer system may be used for de novo sequencing of a nucleic acid sequence by analyzing the fluorescence intensities of hybridized nucleic acid probes on biological chips. The probes with the highest intensities are utilized to sequence the nucleic acid...

20060194206 - Diagnostics of diarrheagenic escherichia coli (dec) and shigella spp: A method for the identification of the diarrheagenic E. coli groups: ETEC (enterotoxigenic E. coli), A/EEC (attaching and effacing E. coli) EPEC (enteropathogenic E. coli), VTEC (verocytotoxin producing E. coli) and EIEC (enteroinvasive E. coli), and Shigella spp. is described. The bacterial identification is made possible by the specific detection...

20060194228 - Dna sequence recognition: To detect the presence of a match of a target DNA base sequence with a probe DNA base sequence, a single strand of the probe DNA base sequence is prepared. One end of the single strand probe DNA base sequence is linked to an electrode and the other end to...

20060194245 - Expression miniarrays and uses thereof: The present invention provides methods and devices for making new and inexpensive miniarrays suitable for gene expression analysis. Also provided herein are methods of diagnosis for specific tissue or condition using specialized diagnostic miniarrays that exhibit specific visual pattern as diagnostic readout....

20060194223 - Fast method for detecting micro-organisms in food samples: The present invention relates to a specific method accomplishing fast and specific identification of contaminating micro-organisms in large amounts of food stuffs. A method has been developed based on random genome fragments or Zipcode oligonucleotides and DNA microarray technology that overcomes the disadvantages of whole-genome DNA-DNA hybridisation. In particular, the...

20060194204 - Gene expressed in prostate cancer, methods and use thereof: A polypeptide is disclosed that is specifically detected in the cells of the prostate, termed Splice Variant-Novel Gene Expressed in Prostate (SV-NGEP). Polynucleotides encoding SV-NGEP are also disclosed, as are vectors including these poynucleotides. Host cells transformed with these polynucleotides are also disclosed. Antibodies and immunoconjugages are disclosed that specifically...

20060194230 - Genetic markers associated with benign prostatic hyperplasia: The present invention regards expression profiles of one or more nucleic acids indicative of the presence of, susceptibility to, and/or predicting response to therapy of benign prostatic hyperplasia (BPH) in an individual. The present invention identifies pathways not previously associated with BPH, therefore presenting novel diagnostic and therapeutic targets for...

20060194247 - Glycosylated specificity exchangers: The present invention is directed to ligand/receptor and antigen/antibody specificity exchangers comprising a saccharide or glycoconjugate. Methods of making these specificity exchangers and methods of using said specificity exchangers to treat or prevent human disease are described herein....

20060194227 - Heteroduplex tracking assay: A change in viral tropism occurs in many HIV positive individuals over time and may be indicated by a shift in coreceptor use from CCR5 to CXCR4. The shift in coreceptor use to CXCR4 has been shown to correlate with increased disease progression. In patients undergoing HAART, the predominant populations...

20060194200 - High throughput detection of glutathione s-transferase polymorphic alleles: A high-throughput assay for characterizing a subject's genetic makeup is disclosed. Specifically, a high-throughput assay utilizing PCR is disclosed that permits the rapid and accurate characterization of a subject's inherited alleles of the polymorphic glutathione S-transferase (GST) genes GSTM1, GSTM3, GSTP1, and GSTT1. This method allows detection of the specific...

20060194235 - Hlj1 gene expression: The human HLJ1 tumor suppressor gene is herein defined as regulated by promoter, enhancer, and silencer regions. HLJ1 promoter activity and gene expression are inversely correlated with metastatic ability. HLJ1 is highly expressed, and inducible, in cells with low metastatic ability and expressed to a lesser extent in highly metastatic...

20060194242 - Immunotherapy for immune suppressed patients: The present invention provides compositions of a natural cytokine mixture (NCM) for treating a cellular immunodeficiency characterized by T lymphocytopenia, one or more dendritic cell functional defects such as those associated with lymph node sinus histiocytosis, and/or one or more monocyte functional defects such as those associated with a negative...

20060194218 - Inducible fluorescence assay: In one aspect, the invention provides fluorescent probes and assays. The probes include a fluorophore-quencher pair that undergoes a switch from dark to fluorescent in response to a reaction of the quencher. The switch of the probe from dark to fluorescent is typically mediated by an enzyme that acts directly...

20060194207 - Kit for detecting nucleic acids: The present invention provides a reactor for carrying out in a single container the steps of the extraction of nucleic acids and the amplification of a nucleic acid, the procedures of the steps being usually different to each other. The reactor according to the present invention is a reactor for...

20060194241 - Lactobacillus specific probes: Provided herein are sequence primers and probe for the detection of Lactobacillus species as well as diagnostic kit thereof....

20060194233 - Ligand of the protein \"beacon\": The present invention relates generally to a ligand for a protein associated with modulating obesity, diabetes and metabolic energy levels in animals and humans and to genetic sequences encoding the ligand. More particularly, the present invention is directed to a ligand of the protein “beacon” and its homologues. The identification...

20060194209 - Method for controlled release of enzymatic reaction components: Compositions and reaction kits are provided for controlling an enzymatic process. The compositions and kits include a substantially water-insoluble, water-permeable polymer and a poorly water-soluble salt of an essential ionic enzymatic reactant. The disclosed compositions and methods are particularly useful for improving the specificity and performance of PCR....

20060194237 - Method for diagnosing peripheral neuropathy: Genes whose expression is correlated with the presence of CIDP or vasculitic neuropathy are disclosed. Probes and sets of nucleic acids and proteins specific for these genes are described, as are molecular and immunological methods for aiding in the diagnosis of these disease conditions in a subject....

20060194199 - Method for diagnosing testicular seminomas: Objective methods for detecting and diagnosing testicular seminoma (TS) arc described herein. In one embodiment, the diagnostic method involves the determining a expression level of TS -associated gene that discriminate between TS and nomal cell. The present invention further provides methods of screening for therapeutic agents useful in the treatment...

20060194208 - Method for the detection of cytosine methylations in dna with the aid of scorpion: The invention relates to a method for analyzing cytosine methylations in DNA sequences, according to which non-methylated cytosines are first converted into uracil while 5-methylcytosine remains unmodified. The DNA is then amplified by means of a polymerase and at least one primer whose 5 end is connected to a probe...

20060194202 - Method for the manufacture of nucleic acid molecules: The present invention is related to a method for the manufacture of a nucleic acid molecule comprising the steps of a) providing a first at least partially double-stranded oligonucleotide which has a modification allowing the oligonucleotide to be coupled to a surface, whereby the oligonucleotide comprises a recognition site for...

20060194217 - Method of genotyping and phenotyping hepatitis b viruses resistant to antiviral molecules: The invention provides a method of preparation of HBV genomic amplicons which makes it possible, using the same amplicon obtained from a patient, to evaluate HBV strains resistant to antiviral agents by analysing, their genotype, their phenotype and their replicative capacity. The method of the invention guarantees to obtain the...

20060194248 - Method of measuring human cyp3a inducibility: A method for measuring human CYP3A inducibility upon administration of a test drug, characterized in that a non-human animal to which a test drug is administered or a population of human cells cultured in a medium containing a test drug is infected with viruses (A) and (B); virus (A) being...

20060194216 - Methods and compositions for assessing nucleic acids and alleles: The present invention is directed to methods and compositions for evaluating allelic variations with improved quality controls, methods of preparing such compositions, and applications employing such compositions and methods. In particular, the present invention provides methods and compositions for reducing false positives and/or false negatives in nucleic acid measurements....

20060194214 - Methods for assembly of high fidelity synthetic polynucleotides: Disclosed are methods of manufacturing synthetic DNAs, that is, DNAs made at least in significant part by chemical synthesis of polynucleotide polymers. Also provided are methods for assembling plural DNAs in the same pool by multiplexed assembly of synthetic oligonucleotides. In exemplary embodiments, the methods involve pre-amplification of one or...

20060194236 - Methods for generating hypermutable microbes: Bacteria are manipulated to create desirable output traits using dominant negative alleles of mismatch repair proteins. Enhanced hypermutation is achieved by combination of mismatch repair deficiency and exogenously applied mutagens. Stable bacteria containing desirable output traits are obtained by restoring mismatch repair activity to the bacteria....

20060194243 - Methods for identifying dna copy number changes: Methods and computer software products for identifying changes in genomic DNA copy number are disclosed. Methods for identifying homozygous deletions and genetic amplifications are disclosed. Genomic DNA is amplified generically and amplified sample is hybridized to an expression array. The expression array comprises probes to regions of genes that are...

20060194234 - Methods for identifying small molecules that bind specific rna structural motifs: The present invention relates to a method for screening and identifying test compounds that bind to a preselected target ribonucleic acid (“RNA”). Direct, non-competitive binding assays are advantageously used to screen bead-based libraries of compounds for those that selectively bind to a preselected target RNA. Binding of target RNA molecules...

20060194211 - Methods for prognosis and treatment of solid tumors: Solid tumor prognosis genes, and methods, systems and equipment of using these genes for the prognosis and treatment of solid tumors. Prognosis genes for a solid tumor can be identified by the present invention. The expression profiles of these genes in peripheral blood mononuclear cells (PBMCs) are correlated with clinical...

20060194225 - Methods, compositions, and kits for forming self-complementary polynucleotides: The present teachings generally relate to methods, kits, and compositions for detecting target polynucleotide sequences. The teachings also relate to ligation and amplification reactions that generate self-complementary polynucleotide products. In some embodiments, ligation reactions are performed with probes that result in the formation a self-complementary ligation product. Ligation of a...

20060194215 - Methods, reagents and kits for reusing arrays: Methods for stripping and reusing arrays are disclosed. Also disclosed are reagents and kits comprising control target molecules for determining the efficacy of a stripping condition to which an array has been exposed....

20060194239 - Mitochondrial complex i mutations in parkinson's disease and aging: The present invention provides methods for diagnosing and treating Parkinson's disease based on mitochondrial mutations. The present invention also provides methods for diagnosing and treating other diseases and disorders based on mitochondrial mutations....

20060194226 - Modified cyanovirin protein: This disclosure relates to a modified version of cyanovirin-N protein and a transformed Lactobacillus bacterium that is capable of recombinantly expressing this modified protein. Further disclosed are polynucleotide sequence encoding the modified cyanovirin-N protein, expression cassette that allows the expression of the modified protein, and method of make and use...

20060194210 - Novel therapeutic targets in cancer: The present invention relates to novel sequences for use in detection, diagnosis and treatment of cancers, especially lymphomas. The invention provides cancer-associated (CA) polynucleotide sequences whose expression is associated with cancer. The present invention provides CA polypeptides associated with cancer that are present on the cell surface and present novel...

20060194232 - Nucleotide compositions and uses thereof: The present invention relates to preparation of nucleotide compositions and uses thereof for conducting nucleic acid analyses. The compositions and methods embodied in the present invention are particularly useful for nucleic acid analyses that require high-resolution detection of labeled nucleotides or labeled nucleic acid targets....

20060194203 - Packing cell system for eb virus vector: A packaging cell is constructed by preparing a packaging cell carrying an EB virus gene lacking a packaging signal but not carrying a wild type EB virus gene having the packaging signal, and introducing an amplicon plasmid having the packaging signal but having no viral replication ability into the cell....

20060194238 - Peripheral neuropathy diagnosis: Genes whose expression is correlated with the presence of CIDP or vasculitic neuropathy are disclosed. Probes and sets of nucleic acids and proteins specific for these genes are described, as are molecular and immunological methods for aiding in the diagnosis of these disease conditions in a subject....

20060194249 - Process for the detection of chromosomal aberrations in interphase nuclei: The present invention relates to a process for the detection of chromosomal aberrations using e.g. a high resolution multicolor-banding (MCB) technology....

20060194219 - Secreted and transmembrane polypeptides and nucleic acids encoding the same: The present invention is directed to novel polypeptides and to nucleic acid molecules encoding those polypeptides. Also provided herein are vectors and host cells comprising those nucleic acid sequences, chimeric polypeptide molecules comprising the polypeptides of the present invention fused to heterologous polypeptide sequences, antibodies which bind to the polypeptides...

20060194231 - Suspension arrays of cross-reactive oligonucleotide-based sensors: Cross-reactive arrays on encoded beads are used to correlate ‘fingerprints’ of urine, serum and other biological liquids to disease states. Fluorescent hydrophobic sensors are based on nucleic acid three-way junctions, and beads may be encoded by size (could be registered by light scattering) and fluorescence in combination with flow cytometry...

20060194213 - Synthesis of 3'-, or 5'-, or internal methacrylamido-modified oligonucleotides: New modifiers were synthesized for incorporation of a methacrylic function in 3′-, 5′- and internal positions of oligonucleotides during solid phase synthesis. A modifier was used for synthesis of 5′-methacrylated oligonucleotides for preparation of microarrays by a co-polymerization method....

20060194212 - Treatment of aids: The invention includes methods of treating HIV infection in a patient where the method includes administration of an antibody to TNF-alpha and an antibody to interferon-gamma to the patient and administering antiretroviral therapy to a patient. The invention further includes methods of treating HIV infection in a patient where the...

20060194221 - Treatment of herpes: The present invention includes methods for the treatment of a herpes simplex virus infection comprising the administration of inhibitors of gamma interferon, tumor necrosis alpha and interleukin-1....

20060194222 - Triplex probe compositions and methods for polynucleotide detection: The present invention provides composition and methods for the detection and measurement of target nucleic acids. The probes of the present invention, or triplex probes, comprise a complex of three oligonucleotide probes including: (1) a first oligonucleotide probe, (2) a second oligonucleotide probe, and (3) a bridging oligonucleotide probe. In...

20060194246 - Use of predetermined nucleotides having altered base pairing characteristics in the amplification of nucleic acid molecules: The present invention provides Improved methods for amplifying a nucleic acid molecule. More specifically, the invention provides methods for nucleic acid amplification which use primers having equivalent priming efficiency....

20060194244 - Uses of a chemokine receptor for inhibiting hiv-1 infection: This invention provides a polypeptide comprising a fragment of a chemokine receptor capable of inhibiting HIV-1 infection. In an embodiment, the chemokine receptor is C-C CKR-5. In another embodiment, the fragment comprises at least one extracellular domain of the chemokine receptor C-C CKR-5. This invention further provides different uses of...

20060194253 - A transfected cell line and a method using the same for the high throughput screening for inhibitors of the t-type calcium channel alpha-1h: The present invention relates to a cell line in which a potassium channel is introduced. More specifically, it is a cell line capable of activating the T-type α1H calcium channel by depolarization wherein a vector encoding a potassium channel is introduced. Since the cell line of the present invention is...

20060194256 - Compositions containing, methods involving, and uses of non-natural amino acids and polypeptides: Disclosed herein are non-natural amino acids and polypeptides that include at least one non-natural amino acid and methods for making such non-natural amino acids and polypeptides. The non-natural amino acids, by themselves or as a part of a polypeptide, can include a wide range of possible functionalities, but typical have...

20060194257 - Mecp2e1 gene: The invention is a novel MECP2E1 splice variant and its corresponding polypeptide. The invention also includes methods of using these nucleic acid sequences and proteins in medical diagnosis and treatment of neuropsychiatric disorders or development disorders....

20060194254 - Method for identifying optimal binding ligands to a receptor: The present invention provides a method for determining binding of a receptor to one or more ligands. The method consists of contacting a collective receptor variant population with one or more ligands and detecting binding of one or more ligands to the collective receptor variant population. The collective receptor variant...

20060194251 - Method of fixing low-molecular compound to solid-phase support: A method of fixing a low-molecular compound to a solid-phase support, which comprises a step in which a solution containing a low-molecular compound is brought into contact with a solid-phase support having a photoreactive compound bonded to the surface, a step in which the solution containing a low-molecular compound is...

20060194252 - Nanoscale patterning and immobilization of bio-molecules: A support for immobilizing target molecules comprises a substrate having a plurality of binding regions for binding select target molecules, with target-molecule-capturing agent immobilized at the binding regions. The binding regions are intersperse among other non-binding regions. The binding regions are of sub-micron size, have high selectivity and high binding...

20060194255 - Parallel patch clamp system: A system for high-throughput analysis of membranous samples having ion channels including at least one membranous sample, a multi-compartment structure including an extracellular chamber, an opposing intracellular chamber and a partition separating the extracellular and intracellular chambers, the partition having a plurality of apertures fluidly and electrically coupling the extracellular...

20060194258 - Polypeptide array synthesis: The present invention provides methods and apparatus for sequencing, fingerprinting and mapping biological macromolecules, typically biological polymers. The methods make use of a plurality of sequence specific recognition reagents which can also be used for classification of biological samples, and to characterize their sources....

20060194259 - Method for isolating an allosteric effector of a receptor: A method for isolating an allosteric effector of a receptor, by determining the variation of the dissociation kinetics of the complex formed between the receptor and one of its ligands in the presence of the allosteric effector, as compared to the kinetics dissociation, in the absence of the effector, and/or...

20060194261 - Methods for determining cellular response to stimuli: The present invention provides a method for determining cellular response to stimuli. The cells to be tested, for example, may be contained in a section of taste-bud containing lingual epithelium....

20060194260 - Screening assays for cannabinoid-ligand-type modulators of gpr55: Screening assays for compounds that modulate the interaction between cannabinoids and the GPR55 receptor are disclosed. Furthermore, a method for determining the selectivity of a test compound against a panel of cannabinoid receptors including GPR55 is disclosed....

20060194265 - Novel therapeutic targets in cancer: The present invention relates to novel sequences for use in detection, diagnosis and treatment of cancers, especially lymphomas. The invention provides cancer-associated (CA) polynucleotide sequences whose expression is associated with cancer. The present invention provides CA polypeptides associated with cancer that are present on the cell surface and present novel...

20060194266 - Use of protein rla-0 as a marker for colorectal cancer: The present invention relates to the diagnosis of colorectal cancer. It discloses the use of protein RLA-0 (60S acidic ribosomal protein P0) in the diagnosis of colorectal cancer. It relates to a method for diagnosis of colorectal cancer from a liquid sample, derived from an individual by measuring RLA-0 in...

20060194267 - Methods and kit for diagnosing tick borne illnesses: ELISA, Western Blot, and a peptide-based ELISA were applied to clinical specimens from patients with clinical symptoms of tick borne diseases, including Lyme disease. Peptides from different components of Borrelia during different cycles, including peptides from outer surface protein, leukocyte function associated antigens, immunodominant antigens, variable major proteins, and peptides...

20060194262 - P27 ubiquitination assay and methods of use: This invention relates to easy and reliable assays for the ubiquitination of p27. Because the assay can be performed as a plate capture assay, or as a homogenous time-resolved fluorescence resonance energy transfer assay, with defined, easily replicatable reaction conditions, it is particularly useful for high-throughput screening of, for example,...

20060194264 - Affinity based system for detecting particulates in a fluid: This invention provides methods and apparatus for detecting and quantifying particulate matter suspended in a fluid. Specifically, the invention provides an integrated, affinity-binding based, analytical system comprising a platform for performing an affinity-binding based assay for specifically binding particulates including microbial cells, and a detection means for detecting the particulates...

20060194263 - Small molecule mediated, heterogeneous, carbon nanotube biosensing: Nanosensors for detecting ananlytes and methods of detecting analytes have been developed in which a small molecule effector concentration is altered thereby causing changes in carbon nanotube conductance....

20060194268 - Method for measuring spinorphin: The present invention aims at providing a method for measuring spinorphin in a sample collected from a living body accurately with high sensitivity. The present invention provides a method for measuring spinorphin in a sample collected from a living body, comprising the steps of: (A) mixing a sample collected from...

20060194269 - Polysaccharide structure and sequence determination: The invention provides a method for the structural analysis of a saccharide, comprising: a) providing on a surface a plurality of essentially sequence- and/or site-specific binding agents; b) contacting said surface with a saccharide to be analyzed, or with a mixture comprising a plurality of fragments of said saccharide; c)...

20060194270 - Method for measuring oxidized ldl-crp complex and measurement kit: The present invention provides a method for measuring a “complex of oxidized LDL and CRP” which comprises using an “anti-CPR antibody”. An “anti-apoB antibody and/or anti-β2-GPI antibody” is preferably used in the method of the present invention, which preferably comprises at least a step for forming a sandwich complex represented...

20060194271 - Phosphorylation and regulation of akt/pkb by the rictor-mtor complex: In certain aspects, the invention relates to methods for identifying compounds which modulate Akt activity mediated by the rictor-mTOR complex and methods for treating or preventing a disorder that is associated with aberrant Akt activity....

20060194272 - Reference material for assaying d-dimer: The present invention relates to a reference material for assaying D-dimer, comprising a fibrin degradation product, wherein no less than 70% of a total peak area has the molecular weight of no less than 500,000 in a molecular weight distribution of the fibrin degradation product, measured by gel filtration chromatography....

20060194273 - Microfabricated apparatus for cell based assays: Apparatus and methods are provided for performing cell growth and cell based assays in a liquid medium. The apparatus comprises a base plate supporting a plurality of micro-channel elements, each micro-channel element comprising a cell growth chamber, an inlet channel and an outlet channel, a cover plate positioned over the...

20060194274 - Method for producing ketocarotinoids in genetically modified, non-human organisms: The present invention relates to a process for the preparation of ketocarotenoids by culturing genetically modified organisms which in comparison with the wild-type have a modified ketolase activity and a modified β-cyclase activity, to the genetically modified organisms, and to their use as foodstuffs and feedstuffs for the production of...

20060194286 - Apo-2li and apo-3 polypeptides: Novel polypeptides, designated Apo-3 and Apo-2LI, involved in apoptosis are provided. Compositions including chimeric molecules, nucleic acids, and antibodies are also provided....

20060194285 - Biological production of clavulanic acid and related compounds: Disruption of the two-component regulatory system encoded by the cbsA locus is associated with improved production of cephamycin C and/or clavulanic acid in Streptomyces clavuligerus....

20060194287 - Chemokine-binding protein and methods of use: Described herein are chemokine-binding domains of THAP-family polypeptides and pharmaceutical compositions which include a polypeptide comprising a chemokine-binding domain of a THAP-family polypeptide. Also disclosed are methods of binding chemokines, inhibiting the activity of chemokines, detecting chemokines, and reducing the symptoms associated with a chemokine mediated or influenced condition by...

20060194282 - Fluorescent protein variants and methods for making same: The present invention relates generally to variant fluorescent proteins, and more specifically to monomeric and dimeric forms of Anthozoan fluorescent proteins. In one aspect, the present invention provides variants of fluorescent proteins, where the variants have a reduced propensity to tetramerize, and form dimeric or monomeric structures. The invention also...

20060194277 - Method for producing target proteins by deleting or amplifyingibpa and/or ibpb gene coding for inclusion body-associated proteins: A method for producing target proteins by deleting or amplifying ibpA and/or ibpB genes coding for inclusion body-associated proteins. Two methods for producing target proteins using ibpA and/or ibpB genes coding for inclusion body-associated proteins of E. coli are described, The first method enhances the secretory production and activity of...

20060194289 - Method of production of recombinant proteins in eukaryote cells: The invention provides a method for production of polypeptides in eukaryote microcarrier cell culture, the method comprising the steps of (i) Culturing cells expressing said polypeptide on microcarriers under conditions and at a setpoint temperature appropriate for expression of said polypeptide; (ii) Cooling the culture to a predetermined temperature below...

20060194280 - Methods for refolding of recombinant antibodies: The present invention is generally directed to methods of producing an increase in the enrichment or recovery of preferred forms of IgG proteins. More particularly, the invention relates to subjecting preparations of such recombinant IgG proteins with a reduction/oxidation coupling reagent and optionally a chaotropic agent....

20060194281 - Novel hcv core‘protein, methods for diagnosis of hcv infections, prophylaxis, and for screening of anti-hcv agents: The present invention relates to a novel form of core+1 protein of Hepatitis C virus (HCV), designated shorter form core+1 protein. The shorter form core+1 protein of Hepatitis C virus is the product of translation of a coding sequence consisting of all or part of a nucleotide sequence extending from...

20060194288 - Nucleic acid molecules encoding endonucleases and methods of use thereof: We describe here an in vitro method of increasing complementarity in a heteroduplex polynucleotide sequence. The method uses annealing of opposite strands to form a polynucleotide duplex with mismatches. The heteroduplex polynucleotide is combined with an effective amount of enzymes having strand cleavage activity, 3′ to 5′ exonuclease activity, and...

20060194290 - Polypeptide variants with altered effector function: The present invention concerns polypeptides comprising a variant Fc region. More particularly, the present invention concerns Fc region-containing polypeptides that have altered effector function as a consequence of one or more amino acid modifications in the Fc region thereof....

20060194291 - Polypeptide variants with altered effector function: The present invention concerns polypeptides comprising a variant Fc region. More particularly, the present invention concerns Fc region-containing polypeptides that have altered effector function as a consequence of one or more amino acid modifications in the Fc region thereof....

20060194278 - Process for producing glucose dehydrogenase: A glucose dehydrogenase complex is produced by culturing an Escherichia bacterium, which is introduced with DNAs encoding the α-subunit and the β-subunit of glucose dehydrogenase of Burkhorderia cepacia in an expressible form, and in which expression of the ccm system (cytochrome c maturation system) is enhanced, to express the aforementioned...

20060194279 - Protein-refolding material: The present invention provides a method for refolding protein produced, for example, by Escherichia coli, that is inactive due to an as yet unformed higher order structure, or protein deactivated due to a change in conformation for some reason. The invention comprises a method, refolding kit, refolding agent, and molding...

20060194284 - Recombinant co-expression of vitamin k epoxide reductase subunit 1 to improve vitamin k dependent protein expression: The present invention relates to a host organism containing recombinant nucleic acids coding for the vitamin K reductase complex subunit 1 (VKORC1) and recombinant nucleic acids coding for a vitamin K dependent (VKD) protein, wherein both the recombinant VKORC1 and the recombinant VKD protein are expressed in said host organism....

20060194283 - Secreted and transmembrane polypeptides and nucleic acids encoding the same: The present invention is directed to novel polypeptides and to nucleic acid molecules encoding those polypeptides. Also provided herein are vectors and host cells comprising those nucleic acid sequences, chimeric polypeptide molecules comprising the polypeptides of the present invention fused to heterologous polypeptide sequences, antibodies which bind to the polypeptides...

20060194276 - Specificity grafting of a murine antibody onto a human framework: The current invention provides new human variable chain framework regions and humanized antibodies comprising the framework regions. The invention also provides a new method of identifying framework acceptor regions in framework sequences for backmutation to graft a donor sequence to the human framework....

20060194275 - Transporter and ion channels: The invention provides human transporters and ion channels (TRICH) and polynucleotides which identify and encode TRICH. The invention also provides expression vectors, host cells, antibodies, agonists, and antagonists. The invention also provides methods for diagnosing, treating, or preventing disorders associated with aberrant expression of TRICH....

20060194292 - Growth factor complexes and modulation of cell migration and growth: Isolated protein complexes are provided comprising growth factors such as IGF-I, IGF-II, VEGF or PDGF, or at least domains thereof that enable binding to and activation of both a growth factor receptor, and an integrin receptor-binding domain of vitronectin or fibronectin. These protein complexes may be in the form of...

20060194293 - Rb1 gene induced protein (rb1cc1) and gene: To provide a novel gene and protein involved in multidrug resistance in cancer, to elucidate functions of the gene and protein, to provide methods of detecting the gene and antibody against the protein and of testing and diagnosing cancer using the gene and antibody, we found a novel protein (RB1CC1)...

20060194294 - Process for the recovery of staurosporine from a fermentation broth: Staurosporine of the formula (I) is recovered from a fermentation broth in a process comprising an ultrafiltration and a diafiltration step....

20060194295 - Novel, esterase, fungus capable of producing the same and method for producing the same: The present invention provides a novel esterase derived from Ideonella sp. 0-0013 strain (FERM BP-08660) having the following properties: (1) function, substrate specificity: hydrolyzes methyl 3-hydroxypalmitate to generate 3-hydroxypalmitic acid and methanol; (2) optimal temperature for functioning: 37° C.; (3) optimal pH and stable pH range: pH 7 or more...

20060194296 - Ethanol production process: An improved ethanol production process providing novel stillage treatment is disclosed wherein the stillage is separated into four value added product streams that are subjected to drying conditions reducing or eliminating volatization of any VOC's in the product streams....

20060194297 - Process for the preparation of dihydroxy esters and derivatives thereof: wherein R and R′ represent optionally substituted hydrocarbyl groups and X represents a hydrocarbyl linking group. The process comprises either the stereoselective reduction of the keto group in a dihydroxy keto precursor followed by selective esterification of a primary hydroxy, or selective esterification of a primary hydroxy of a dihydroxy...

20060194299 - Method for recycling important nutritional elements from waste: The present invention relates to a process wherein waste material derived from human, animal and industrial areas is processed to utilize the energy resources present in the solid phase and, optionally, to recover important nutritional elements as well as toxic heavy metals. In particular, there is provided a process for...

20060194298 - Phytase produced from citrobacter braakii: The present invention relates to novel phytase enzyme, a gene coding the enzyme, and a Citrobacter sp. producing the enzyme. Particularly, the present invention relates to the phytase enzyme produced from Citrobacter sp. having (a) molecular weight of 47 kDa, (b) optimal pH of 3.5-4.5, (c) optimal temperature of 45-55°...

20060194300 - Process for preparing an alpha-1-antitrypsin solution: A process for preparing A1AT from A1AT-containing solutions, comprising the following steps: (a) subjecting an A1AT-containing solution to ion-exchange chromatography; (b) adding detergents and optionally a solvent for inactivating lipid-enveloped viruses; (c) followed by increasing the salt concentration to salt out the detergents. A1AT having a purity of >90% with...

20060194301 - Large-scale production of human serum butyrylcholinesterase as a bioscavenger: Disclosed herein are methods for the large-scale preparation of human butyrylcholinesterase (HuBChE) preparations from Cohn Fraction IV-4. As disclosed, the methods provide HuBChE preparations that are about 99% or more pure with recovery yields of about 60%. Also disclosed are the pharmacokinetics, safety and toxicity, stability and efficacy of the...

20060194302 - Method for producing amino acids by fermentation: The present invention provides a method for producing an amino acid selected from the group consisting of L-alanine, L-valine, L-leucine, L-isoleucine, L-methionine, L-phenylalanine, L-proline, glycine, L-serine, L-threonine, L-cysteine, L-tyrosine, L-asparagine, L-glutamine, L-lysine, L-histidine, L-arginine, L-aspartic acid and L-glutamic acid and useful as medicament, chemical agent, food material and feed additive...

20060194304 - Process for the depletion or removal of endotoxins: A process for the depletion or removal of endotoxins from preparations containing active ingredients designated for therapeutical use which are obtained from natural sources by genetic engineering and/or biotechnology by treatment with chromatographic material wherein said natural sources are lysed, the fractions obtained are optionally centrifuged, filtrated or treated with...

20060194303 - Wastewater treatment compositions: The present invention relates to a wastewater treatment composition and a process of decolorizing pulp and paper mill wastewater and, more specifically, to a process for treating wastewater effluent from a pulp or paper mill with a microorganism whereby color bodies in the pulp and paper wastewater are thereby removed...

20060194305 - Micro-holding pipette with an oblique opening: The present invention relates to a micro-holding pipette. The holding end of the micro-holding pipette is a oblique opening, i.e. the end surface of the opening of the holding end of the micropipette is at an acute angle with the ordinate axis of the holding end, which is less than...

20060194306 - System for gel electrophoretic immunoassay: A micro-analytical platform for performing electrophoresis-based immunoassays was developed by integrating photopolymerized cross-linked polyacrylamide gels within a microfluidic device. The microfluidic immunoassays are performed by gel electrophoretic separation and quantifying analyte concentration based upon conventional polyacrylamide gel electrophoresis (PAGE). To retain biological activity of proteins and maintain intact immune complexes,...

20060194307 - Cell measuring and sorting chip: The present invention provides a cell sorting device using an inexpensive chip capable of being exchanged for each sample for cell sorting. The cell sorting device uses flow paths formed on the substrate and has the following structure. The chip includes: a first flow path allowing a buffer fluid containing...

20060194308 - Imaging fluorescence signals using telecentricity: The present invention relates to the field of DNA analysis. In particular, the present invention is directed to a device for the parallel imaging of fluorescence intensities at a plurality of sites as a measure for DNA hybridization. More particular, the present invention is directed to a device to image...

20060194309 - Method and device for the formation of biological cell material: A method for producing cell material (20) having multiple biological cells (21), which have a predefined geometrical arrangement, includes the steps of providing a manipulation tool (10) having a tool body (11), whose surface (12, 14) at least partially contacts the cell material (20), and adjusting the manipulation tool (10)...

20060194310 - Cell culture spinner flasks: The present invention relates to culturing devices that are compact, utilize small amounts of cell culture media to establish and maintain cell cultures, and produce a large number of cells in a short period of time when compared to other cell culturing devices and techniques. Such devices are useful in...

20060194311 - Lna-cpg conjugates: The present invention relates to novel compositions comprising DNA attached to an immunostimulatory oligonucleotide (CpG) via a locked nucleic acid oligonucleotide of a defined sequence. In particular the present invention provides compositions comprising a plasmid containing a gene encoding a protein of interest, wherein said plasmid may be introduced to...

20060194312 - Method of cell culture observation, carbon substrate for cell culture observation, and method for manufacture thereof: The invention provides a substrate suitable for cell culture observation and a method of observation using the same. Crystalline carbon such as a graphite powder is mixed into a thermosetting resin such as a furan resin, and the mixture is molded in the shape of a sheet and carbonized to...

20060194314 - Novel bt toxin receptors from lepidopteran insects and methods of use: The invention relates to Bt toxin resistance management. The invention particularly relates to the isolation and characterization of nucleic acid and polypeptides for a novel Bt toxin receptor. The nucleic acid and polypeptides are useful in identifying and designing novel Bt toxin receptor ligands including novel insecticidal toxins....

20060194313 - Shear protectants in harvest microfiltration: The addition of buffer to a harvest stream during diafiltration can cause increased turbidity and have other undesirable effects including limiting product recovery. Methods and compositions related to the use of a non-ionic surfactant are provided for improving diafiltration....

20060194315 - Compositions and methods for the control, differentiaton and/or manipulation of pluripotent cells through a gamma-secretase signaling pathway: The current invention relates to the control and/or manipulation of the gamma-secretase signaling pathway in pluripotent cells to stabilize the cells in a pluripotent state and/or to control the differentiation of the pluripotent cells towards a differentiated state. The invention further includes feeder layers that contain or express ligands or...

20060194316 - Bone marrow-derived neuronal cells: Bone marrow stromal cells (BMSC) differentiate into neuron-like phenotypes in vitro and in vivo, engrafted into normal or denervated rat striatum. The BMSC did not remain localized to the site of the graft, but migrated throughout the brain and integrated into specific brain regions in various architectonic patterns. The most...

20060194317 - Expression of apoai and variants thereof using spliceosome mediated rna trans-splicing: Methods and compositions for generating novel nucleic acid molecules through targeted spliceosome mediated RNA trans-splicing that result in expression of a apoAI protein, an apoAI variant, the preferred embodiment referred to herein as the apoAI Milano variant, a pre-pro-apoAI or an analogue of apoAI. The methods and compositions include pre-trans-splicing...

20060194318 - Cultured cd14+ antigen presenting cells: The present invention provides isolated CD14+ antigen presenting cells, e.g., dendritic cells and isolated and enriched populations thereof as well as methods for isolation and enrichment. Also provided are methods for using the CD14+ antigenpresenting cells to modulate T cell responses in vivo, in vitro, and ex vivo....

20060194319 - Promoter substitution for immunoglobulin therapy: The present invention involves the identification of Bright as involved in immunoglobulin production, and the targeting of that function for the treatment of disease states associated with pathologic immunoglobulin production. Also provided are methods of identifying candidate substances with Bright-inhibitory activity....

20060194320 - Artificial immune organ: The invention relates to methods and devises for the faithful modeling of organ functions in vitro....

20060194321 - Directed differentiation of embryonic stem cells and uses thereof: The present invention provides methods for the directed differentiation of embryonic stem cells along the endodermal lineage, especially the pancreatic lineage....

20060194322 - Culture media compositions free of fetal bovine serum: A cell culture growth media free of Fetal Bovine Serum for use with parasitic organisms. The media includes calcium chloride, sodium bicarbonate, potassium chloride, sodium chloride, monosodium phosphate, glucose, hepes, ferric nitrate, magnesium sulfate, tricine, d-ribose, 2-deoxy ribose, adenosine-5-triphosphate (ATP), 2-deoxyadenylic acid (d-AMP), 5′-thymidylic acid (TMP), 2′-deoxyicitidine-5 monophosphate (d, 2′-deoxyuridine-5-monophosphate...

20060194323 - Defined systems for epithelial cell culture and use thereof: The present invention provides cell culture media formulations which support the in vitro cultivation of animal epithelial cells. The media comprise at least one fibroblast growth factor (FGF) and at least one agent that induces increased intracellular cAMP levels, and optionally comprise ascorbic acid. The present invention also provides methods...

20060188865 - Sample holding device and method for the production thereof: The invention relates to a sample holding device (100), particularly for cryopreserving at least one sample, which has a bundle (10) consisting of a multitude of tubular flexible sample compartments (11, 12,) and a retaining device (20), to which the bundle (10) of sample compartments is connected. The retaining device...

20060188866 - Diaminophenothiazine compositions and uses thereof: Cell senescence is delayed by contacting a cell specifically determined to be in need of delayed cell senescence with an effective amount of a diaminophenothiazine....

20060188867 - Method of cryopreserving cells and tissues by liposomal delivery of sugars to enhance post-thaw viability: A method for cryopreserving cells entails the liposomal delivery of intracellular sugar(s), such as trehalose, sucrose, raffinose, stachyose, and combinations thereof, into cells and tissues, such as red blood cells, for enhancing post-thaw viability. This method enables rapid and easy delivery of protective molecules into cells which thus greatly simplifies...

20060188868 - Direct detection method for products of cellular metabolism using tof-sims: A rapid and efficient method for novel biological substance screening by surface analysis has been developed using Time-of-Flight Secondary Ion Mass Spectrometry (ToF-SIMS). This method relies on the surface screening of an array of micro-organisms grown on porous membranes, which had previously been in contact with a solid growth medium....

20060188870 - Identification of active sites in enzymes: A method for determining the location of the active site of an enzyme is provided. The method comprising: determining a location of a reference point inside a functional unit of the enzyme; determining a limiting distance from the reference point; and identifying one or more molecular surface portions within the...

20060188869 - Method and apparatus for uv imaging: A method and apparatus for illuminating a subject with deep UV light and detecting 2-dimensional image information, e.g., a transmission image, fluorescence image, reflected light image, etc. The subject may be illuminated with a light pulse having a duration of 2 seconds or less and one or more components with...

20060188871 - Detection of very virulent infectious bursal disease virus: Methods of identifying animals infected with a vvIBDV are provided. The methods comprise contacting a nucleic acid sample obtained from the animal or a nucleic acid product obtained by amplifying RNA obtained from the animal with one or more probe pairs, each of which comprises a mutation probe and an...

20060188873 - Method for detecting a target polynucleotide: A method of detecting a target polynucleotide using a solution phase nucleic acid sandwich assay, wherein the solid support is an optical planar waveguide, and the label is detected by measuring the luminescence that is excited in the evanescent field of the waveguide-A fully automated system may be used. The...

20060188874 - Methods and compositions for colorimetrically assessing peptide characteristics: Methodologies and compositions useful for calorimetrically assessing peptide characteristics. By way of example, the methodologies can be used to qualitatively and quantitatively assess the degree to which a peptide sample incorporates beta sheet content. The colorimetric information also information is useful to predict solubility, formulating, and other peptide characteristics impacted...

20060188872 - Novel post-transcriptional regulatory elements and uses thereof: The invention provides a novel post-transcriptional regulatory element that can function as an RNA nucleo-cytoplasmic transport element. The invention also provides for an attenuated HIV-1 hybrid virus for use as a vaccine and a kit incorporating the hybrid virus. The kit also includes instructional material teaching the use of the...

20060188915 - Atropisomers of asymmetric xanthene fluorescent dyes and methods of dna sequencing and fragment analysis: Atropisomeric energy-transfer dye compounds are disclosed. A variety of molecular biology applications utilize atropisomeric xanthene fluorescent dyes as labels for substrates such as nucleotides, nucleosides, polynucleotides, polypeptides and carbohydrates. Methods include DNA sequencing, DNA fragment analysis, PCR, SNP analysis, oligonucleotide ligation, amplification, minisequencing, and primer extension....

20060188876 - Binding a target substance: Magnetic particles capable of binding a target substance, which comprise a magnetic material and a matrix material, wherein the magnetic material is remanent upon exposure to a magnetic field and the matrix material has a surface comprising functional groups which promote disaggregation of the particles in the presence of a...

20060188922 - Biopolymeric arrays having replicate elements: A method for designing an array is provided. In certain embodiments, this method includes grouping probes into a plurality of ranked groups of probes; and designing an array comprising the ranked groups of probes, wherein the array contains more replicates of probes in a higher ranked group as compared to...

20060188904 - Biosensors having single reactant components immobilized over single electrodes and methods of making and using thereof: Disclosed herein are single reactant components immobilized over single electrodes and methods of making and using thereof. Devices, such as biosensors, comprising the single reactant components immobilized over single electrodes are also disclosed. Assays using the single reactant components immobilized over single electrodes are disclosed as well as databases comprising...

20060188909 - Business methods for assessing nucleic acids: The present invention is directed to methods and compositions for evaluating nucleic acids, methods of preparing such compositions, and applications and business methods employing such compositions and methods. In particular, the present invention provides business methods for operating a gene expression measurement service....

20060188908 - Compositions and methods of use of standardized mixtures: The present invention is directed to methods and compositions for evaluating nucleic acids, methods of preparing such compositions, and applications and business methods employing such compositions and methods. In particular, the present invention provides business methods for operating a gene expression measurement service....

20060188910 - Compositions, methods, and kits for analyzing dna methylation: Compositions, methods, and kits for reducing strand amplification bias using bisulfite treated gDNA are provided. Methods for detecting and for quantitating the amplified bisulfite treated gDNA and inferring the presence, absence, and/or degree of methylation of target cytosine(s) in the gDNA are also provided. Such methods typically employ tailed first...

20060188927 - Compounds and processes for single-pot attachment of a label to nucleic acid: Compounds and methods are provided for a single-pot covalent attachment of a label to nucleic acids comprising forming a covalently attachable labeling reagent for alkylating the molecule. Then, combining the covalently attachable labeling reagent with a mixture containing the molecule, under conditions wherein the labeling reagent has reactivity with the...

20060188879 - Crh responsive genes in cns: The present invention relates generally to therapy and diagnosis of depression. In particular this invention relates to the polypeptides as well as to the polynucleotides encoding these polypeptides, wherein said polypeptides are shown to play a central role in mediating the endocrine response to corticotropin releasing hormone. These polypeptides and...

20060188917 - Device and method for multiple analyte detection: The invention is directed to a method and device for simultaneously testing a sample for the presence, absence, and/or amounts of one or more of a plurality of selected analytes. The invention includes, in one aspect, a device for detecting or quantitating a plurality of different analytes in a liquid...

20060188882 - Diagnostics and therapeutics for diseases associated with phosphodi-esterase 1c (pde1c): The invention provides a human PDE1C which is associated with the cardiovascular disorders, gastrointestinal and liver diseases, cancer disorders, neurological disorders, respiratory Diseases and urological disorders. The invention also provides assays for the identification of compounds useful in the treatment or prevention of cardiovascular disorders, gastrointestinal and liver diseases, cancer...

20060188881 - Dna amplification method and kit therefor: In a method for amplifying a DNA, comprising performing PCR using a sense primer and an antisense primer, PCR is performed in the presence of an additional sense primer comprising the sense primer and an oligodeoxyribonucleotide having a first additional sequence and ligated to the 5′ end of the sense...

20060188884 - Enhanced hiv-1 vaccines and methods for their use: The present invention provides peptides and proteins for use in second generation HIV vaccines and as diagnostic tools in the treatment and control of HIV infection. The antiviral protection shown by compositions of the present invention has not been previously achieved with an HLA epitope-enhanced vaccine. These findings define a...

20060188892 - Enzymatic digestion of tissue: The present invention concerns a compositions and method for isolating a nucleic acid from a cell-containing sample. There is disclosed a method comprising obtaining at least one cell-containing sample, which comprises a cell containing nucleic acid, obtaining at least one catabolic enzyme, obtaining at least one nuclease inhibitor, preparing an...

20060188925 - Feline ctla-4 nucleic acid and polypeptides: The present invention provides isolated and purified DNA encoding feline CD80 (B7-1) ligand, feline CD86 (B7-2) ligand, feline CD28 receptor, or feline CTLA-4 (CD 152) receptor, as well as vectors comprising nucleic acid encoding feline CD80, feline CD86, feline CD28, or feline CTLA-4. The present invention provides a host cells...

20060188877 - Fluorescence-quenching used to detect nucleic acid oligomer hybrization events at high salt concentrations: A method is described for detecting nucleic acid oligomer hybridization events by fluorescence quenching, which comprises as a first step the provision of a modified surface. The modification of the surface consists in the binding of at least one type of modified nucleic acid oligomers 201, wherein said nucleic acid...

20060188928 - Gene causative of rothmund-thomson syndrome and gene product: The RecQ4 helicase gene, belonging to the RecQ helicase gene family, is revealed herein to be the causative gene of Rothmund-Thomson syndrome. The present inventors found out that it is possible to diagnose Rothmund-Thomson syndrome by detecting mutation of this gene. Further, they uncovered that it is possible to treat...

20060188911 - Genetic testing method: A genetic testing method for specifically amplifying a DNA complementary to a target nucleic acid existing in a biologic sample, measuring the amplified DNA, and generating diagnosis support information on the basis of a measurement result is described that includes steps of: (a) specifically amplifying a DNA complementary to an...

20060188899 - High speed dna sequencer and method: This invention relates to a device for the determination of the sequence of nucleic acids and other polymeric or chain type molecules. Specifically, the device analyzes a sample prepared by incorporating fluorescent tags at the end of copies of varying lengths of the sample to be sequenced. The sample is...

20060188900 - High speed nucleic acid sequencing: The present invention is directed to a method of sequencing a target nucleic acid molecule having a plurality of bases. In its principle, the temporal order of base additions during the polymerization reaction is measured on a molecule of nucleic acid, i.e. the activity of a nucleic acid polymerizing enzyme...

20060188885 - High throughput functional genomic screening methods for osteoarthritis: High-throughput functional screening assays are provided that identify genes and gene products that are associated with the pathogenesis of osteoarthritis (OA) in chondrocytes. In addition, genes and gene products identified by such functional assays are also provided. The genes and gene products provided herein are useful inter alia for diagnosing...

20060188875 - Human genomic polymorphisms: The invention provides nucleic acid segments of the human genome including polymorphic sites, SNP haplotype blocks, SNP haplotype patterns for each block and informative SNPs for each pattern. Allele-specific primers and probes hybridizing to regions flanking these sites are also provided. The nucleic acids, primers and probes are used in...

20060188918 - Hybridization method and hybridization equipment: The equipment of the present invention can implement high-speed hybridization with a simple construction and is configured as described below. The equipment comprises a biochip prepared by fixing biopolymers onto a plurality of sites on a substrate, positive and negative electrodes for generating an electric field along the surface of...

20060188920 - Identification of compounds that inhibit replication of human immunodeficiency virus: The present invention relates to the discovery of a novel class of compounds that inhibit the replication of human immunodeficiency virus (HIV) and approaches to identify these compounds. More specifically, it has been found that enzymatically prepared alpha-hydroxyglycinamide and synthetically prepared alpha-hydroxyglycinamide inhibit the replication of HIV in human serum....

20060188903 - Implantation serine proteinases: This invention provides three novel serine proteinases that are important for female fertility, particularly in the process of hatching and implantation. These proteinases, as well as the nucleic acids, fragments, analogs, and/or inhibitors thereof, can be used to modulate hatching, implantation and female fertility in general....

20060188901 - Labelled nucleotides: Nucleosides and nucleotides are disclosed that are linked to detectable labels via a cleavable linker group....

20060188883 - Markers for colorectal cancer: Provided are previously uncharacterised markers of cancers, for example colorectal cancers, and uses of these as diagnostic and prognostic markers of cancers, and in particular colorectal cancers. The markers are SEQ ID NO: 1—hnRNP-K; SEQ ID NO:2—HMG-1; SEQ ID NO:3—proteasome subunit alpha type 1; SEQ ID NO:4—bifunctional purine biosynthesis protein;...

20060188898 - Markers for roscovitine: The present invention relates to pharmacodynamic markers for CDKIs including the candidate 2,6,9-tri-substituted purine known as roscovitine. The identity of these markers facilitates the convenient identification of roscovitine-like activity both in vitro and in vivo....

20060188887 - Method and system for elucidating the primary structure of biopolymers: The present invention relates to a method and to a system (100) for predicting the primary structure of biopolymers, especially of proteins and peptides, in which at least two algorithms or the results of at least two algorithms and/or of bioinformatic analyses are combined in order to increase the significance...

20060188880 - Method for detecting an analyte in a sample: The present invention relates to a method for detecting an analyte in a sample comprising the steps of providing detection probes being labeled with a first reporter, which detection probes are capable of binding to the analyte, providing a solid support, providing capture probes being bound or capable of binding...

20060188894 - Method for screening compounds against flaviviruses by using persistent virus-infected cell system: This invention provides a method for screening compounds against Flavivirus. More specifically, this invention provides a method for screening compounds against Flaviviruses by using a persistent virus-infected cell system, including (a) establishment of persistent virus-infected cell lines, (b) preparation of monoclonal antibody by using said persistent virus-infected cell lines, (c)...

20060188886 - Method of detecting or quantitatively determining mitochondrial dna 3243 variation, and kit therefor: A method for detecting a DNA having the mitochondrial DNA 3243 mutation, using quantitative PCR using a primer having a nucleotide sequence complementary to a nucleotide sequence starting from the nucleotide number 243 in the nucleotide sequence of SEQ ID NO: 2 and having a length of 12 to 30...

20060188888 - Method of screening for improved specific activity of enzymes: This invention relates to a method for screening libraries of enzyme variants for changes in specific activity by expression of a fusion protein consisting of at least two enzymes. By using one enzyme as a marker changes in specific activity for the other enzyme can be screened efficiently....

20060188891 - Methods and apparatus for controlling dna amplification: Apparatus and methods for optimizing DNA amplification are provided. The apparatus of the invention includes a DNA amplification cycler and a detector capable of measuring a parameter of at least one stage of DNA amplification that changes as the reaction of said stage approaches completion. It is presently preferred that...

20060188913 - Methods and products for enhancing immune responses using imidazoquinoline compounds: The invention involves administration of an imidazoquinoline agent in combination with another therapeutic agent. The combination of drugs may be administered in synergistic amounts or in various dosages or at various time schedules. The invention also relates to kits and compositions concerning the combination of drugs. The combinations can be...

20060188921 - Methods for diagnosing and treating systemic lupus erythematosus disease and compositions thereof: The present invention is directed to novel methods for diagnosis and prognosis of Systemic lupus erythematosus by identifying differentially expressed genes. Moreover, the present invention is also directed to methods that can be used to screen test compounds and therapies for the ability to inhibit systemic lupus erythematosus. Additionally, methods...

20060188878 - Methods to predict ederma as a side effect of drug treatment: This invention provides methods to predict the likelihood of occurrence of the side effect of edema in patients treated with a drug including, but not limited to, a TKI, such as Imatinib or GLEEVEC™/GLIVEC®. The methods employed use gene expression profile comparisons and the determination of specific SNPs and in...

20060188906 - Microfluidic chip for multiple bioassay and method of manufacturing the same: Provided are a microfluidic chip for multiple bioassay and a method of manufacturing the same. The method includes: forming microfluidic channels by coupling a channel structure having grooves for sample channels and probe channels in a bottom surface and a substrate; forming probe immobilization regions at intersections between the probe...

20060188923 - Novel human parvovirus b19 receptor and uses thereof: A novel receptor for human parvovirus B19, other than P antigen, as well as a reagent for measuring human parvovirus B19, a reagent for adsorbing human parvovirus B19, and an agent for suppressing infection, which utilize the receptor for human parvovirus B19 is disclosed. It was discovered that Ku 80...

20060188896 - Nucleic acid amplification with integrated multiplex dectection: A method mediated with in-vitro transcription (“IVT”) which permits miniaturization of multiplexed DNA and RNA analysis, and in which elongation-mediated multiplexed analysis of polymorphisms (eMAP®) is used as the analysis step, is described. Also described is a method mediated with IVT is for selecting a designated strand from T7-tagged double...

20060188926 - Nucleic acid sequences and methods for identifying compounds that affect rna/rna binding protein interactions and mrna functionality: Disclosed herein are nucleic acid sequences and their optimized subfragments which are located in the mRNA untranslated regions of therapeutically-relevant genes. These sequences specifically bind RNA binding proteins (RBPs) and/or regulate the mRNA functionality. Also disclosed are methods of optimizing a subfragment of a parent nucleic acid sequence such that...

20060188919 - Nucleic acid sequences having characteristics of enhanced expression in human neuroblastoma with favorable prognosis based on comparison between human neuroblastoma with favorable prognosis and human neuroblastoma with unfavorable prognosis: There are disclosed a nucleic acid which is derived from the gene expressed in human neuroblastoma, and which comprises any sequence selected from the group consisting of the nucleic acid sequences set forth SEQ ID NO:1 to NO:104 in the Sequence Listing, or its complementary nucleic acid; a fragment of...

20060188907 - Patterning method, substrate for biomolecule immobilization using the patterning method, and biochip employing the substrate: Provided is a linker functional group patterning method for biomolecule immobilization. The patterning method includes preparing a coating composition including a hydrophobic group-containing silane compound and a hydrophilic group-containing silane compound; forming a surface tension control layer by coating the coating composition on a substrate for biomoleucle immobilization; and forming...

20060188916 - Peptoids incorporating chemoselective functionalities: Molecules having potential biological activity, particularly peptoids, that are conjugated to solid phase supports, spacer groups, and/or ligation moieties, and methods of their preparation, are described. In some instances, the molecules of the invention are made entirely by solid phase synthesis. In other instances, the spacer groups are hydrophilic and...

20060188902 - Primer for nucleic acid detection: This application provides universal labeled primers for detection and amplification of nucleic acid molecules. These universal primers can be attached to the 5′-end of a target sequence-specific primer. In particular examples, the universal primer includes a labeled nucleotide flanked on both sides a nucleotide whose complement nucleotides changes a detectable...

20060188905 - Process: A process for the preparation of coated polymer particles containing superparamagnetic crystals, said process comprising reacting surface-functionalized, superparamagnetic crystal-containing polymer particles of diameter less than 0.5 μm with at least one polyisocyanate and at least one diol....

20060188895 - Rapid prognostic assay for malignancies treated with epidermal growth factor receptor: A molecular assay for determining the sensitivity or resistance of malignancies to chemotherapy prior to initiation of chemotherapy and which also allows for monitoring the therapeutic effects of the chemotherapy during treatment. The molecular assay measures tumor response to therapy with EGFR modulators and utilizes tumor mRNA as a starting...

20060188893 - Rolling circle amplification of rna: Disclosed are compositions and methods for amplification of RNA molecules. The disclosed method involves synthesizing first strand cDNA molecules from RNA molecules, circularizing the first strand cDNA molecules and replicating the circularized first strand cDNA molecules using rolling circle replication. The method can be aided by the use of specialized...

20060188912 - Sample preparation method incorporating an alkaline shock: Method of preparing a biological sample appropriate for use in a subsequent in vitro nucleic acid amplification reaction. The method involves a rapid, transient exposure to alkaline conditions which can be achieved by mixing an alkaline solution with a pH-buffered solution that includes a detergent and the biological sample to...

20060188914 - Secreted and transmembrane polypeptides and nucleic acids encoding the same: The present invention is directed to novel polypeptides and to nucleic acid molecules encoding those polypeptides. Also provided herein are vectors and host cells comprising those nucleic acid sequences, chimeric polypeptide molecules comprising the polypeptides of the present invention fused to heterologous polypeptide sequences, antibodies which bind to the polypeptides...

20060188924 - Tcl-1 gene and protein and related methods and compositions: The present invention relates to nucleotide sequences of TCL-1 genes and amino acid sequences of their encoded proteins, as well as derivatives and analogs thereof, and antibodies thereto. The TCL-1 gene sequence is preferentially expressed early in T and B lymphocyte differentiation. The present invention further relates to the use...

20060188897 - Transposon mediated differential hybridisation: A method for identifying an essential gene of an organism comprises: (i) providing a Library of transposon mutants of the said organism; (ii) isolating polynucleotide sequences from the library which flank inserted transposons; (iii) hybridising the said polynucleotide sequences with a polynucleotide library from the said organism; and (iv) identifying...

20060188890 - Ubiquitin-based protein interaction assays and related compositions: The invention provides, inter alia, methods for identifying substrates for E3 proteins that mediate ligation of ubiquitin or ubiquitin-like proteins....

20060188889 - Use of differentially expressed nucleic acid sequences as biomarkers for cancer: The present invention relates to novel marker sequences that are differentially expressed in cancer cells or tissue of a subject with cancerous conditions. The present invention also relates to assays for diagnosis, prognosis, staging, monitoring, therapeutic treatment, and marker sequence related agents including probes, primers, antibodies, and therapeutic compositions....

20060188936 - Alzheimer's related proteins and methods of use: Disclosed is a method for identifying substances that alter the interaction of a presenilin protein with a presenilin-binding protein, including contacting at least the interacting domain of a presenilin protein to a presenilin-binding protein in the presence of a test substance, and measuring the interaction of the presenilin protein and...

20060188943 - Color-encoding and in-situ interrogation of matrix-coupled chemical compounds: A method and apparatus for the physico-chemical encoding of a collection of beaded resin (“beads”) to determine the chemical identity of bead-anchored compounds by in-situ interrogation of individual beads. The present invention provides method and apparatus to implement color-coding strategies in applications and including the ultrahigh-throughput screening of bead-based combinatorial...

20060188940 - Combinatorial hydrogel formulation: The present invention concerns a method, apparatus (array assembly), and system for fabricating and formulating hydrogels using combinatorial techniques; a hydrogel array fabricated using the method, apparatus, and/or system of the invention; and methods and systems for testing the properties of arrayed hydrogels, such as bulk and interfacial mechanical properties....

20060188938 - Compounds for inhibiting beta-amyloid production and methods of identifying the compounds: Provided are compounds useful for treating diseases associated with a cerebral accumulation of Alzheimer's amyloid, such as Alzheimer's disease. Also provided are methods for screening for such compounds, by measuring capacitative calcium entry in cells which optionally overexpress APP or a fragment thereof. Also provided are methods of treating or...

20060188942 - Detection of micro-organisms: The present invention concerns methods for detecting the presence of a micro-organism in a fluid, gaseous and solid samples, together with apparatus for use in same. The invention comprises the use of a plurality of hollow fibres to filter the fluid and the capture of the micro-organisms on the membrane...

20060188939 - Fecal sample test device and methods of use: The present invention includes devices and methods for the detection of an analyte in a fecal sample. The fecal sample test device includes a sample collection structure and sample collection housing, a detection housing, a fecal suspension solution or fecal dilution solution, a detection housing capable of attachment to the...

20060188937 - G-protein estrogen membrane receptor: The present invention includes compositions and methods for the identification, isolation and characterization of a G-protein-coupled receptor that binds to an estrogen or estrogen-like molecule, to modulators of the receptor's activity and diagnostic methods for use in detection and treatment of a G-protein-coupled receptor related cancer....

20060188941 - High throughput screening assays utilizing affinity binding of green fluorescent protein: Novel methods of detecting fluorescent proteins are described. The methods result in vastly improved signal-to-noise ratios in assays measuring fluorescence of a fluorescent protein specifically by employing a unique trapping step to microconcentrate the fluorescent protein and by using improved optical techniques. The trapping step may be a chemical or...

20060188933 - Irak-m is a negative regulator of toll-like receptor signaling: Isolated nucleic acid encoding the amino acid sequence of murine IRAK-M; expression vectors comprising nucleic acid encoding IRAK-M and host cells comprising them are disclosed. IRAK-M is induced upon toll-like receptor (TLR) stimulation and negatively regulates TLR signaling. Methods for identifying antagonists and agonists of IRAK-M are described....

20060188930 - Isolated fluorescent protein from clytia gregaria cgfp and use thereof: The inve