Chemistry: molecular biology and microbiology inventions 08/06

20060194191 - Processing method for the long-term stabilization of biological red blood cell volume: The present invention relates to a processing method for long-term stabilization of biological red blood cell volume. The red blood cells can be further used in reference materials and calibrators of a hematology analyzer. The method of the present invention comprises contacting the sample with a solution comprising a membrane-fixation...

20060194192 - Stabilization of cells and biological specimens for analysis: Compositions and methods for stabilizing rare cells in blood specimens, preserving the quality of blood specimens, and also serving as cell fixatives are disclosed which minimize losses of target cells (for example, circulating tumor cells) and formation of debris and aggregates from target cells, non-target cells and plasma components, thereby...

20060194193 - Biological sample culturing and observation system, incubator, supplying device, and culture vessel: The present invention provides a biological sample culturing and observation system which includes: an incubator in which a biological samples are housed, and whose interior is maintained in a culturing environment satisfying predetermined conditions, and the interior is isolated from the outside; an observation optical system that from outside the...

20060194194 - Diagnostic measurement of disease: Diagnostic systems and methods that can be used to perform direct tests on immune system or other proliferative cells, which are capable of identifying abnormal cell function associate with disease without use of labeled reagents; that also permit, but do not require provision of an autoantigen; that are capable of...

20060194197 - Biosensors utilizing dendrimer-immobilized ligands and their use thereof: The present invention is directed to methods and compositions useful as biosensors that specifically interact with various pathogens and other target analytes. The biosensor itself, comprises functionalized dendritic tethers derivatized for attachment to a variety of surfaces as self-assembled monolayers (SAMs) as well as attached binding moieties (sometimes referred to...

20060194195 - Multiple antigenic peptide assay for detection of hiv or siv type retroviruses: A method for detecting at least one antibody directed against at least one primate immunodeficiency virus in a biological sample that includes contacting a biological sample with (i) at least one detection multiple antigenic peptide comprising a portion of an immunodominant region of a transmembrane protein of a primate immunodeficiency...

20060194196 - Novel surface protein (hbsag) variant of the hepatitis b virus: The invention relates to sequences of a novel variant of the Hepatitis B surface antigen (HBsAg) and to methods for detecting, in patient samples, nucleic acids, antigens and antibodies directed against the same....

20060194198 - Use of fibrous protein fibers for chemical sensing and radiation detection: Fibrous protein fibers such as keratin fibers can be used to detect chemicals and radiation. One aspect of the invention is a method of detecting an analyte in a sample comprising the steps of: (1) providing a fiber of fibrous protein; (2) contacting the fiber of fibrous protein with an...

20060194201 - Autism gene: The present invention concerns genes containing mutations associated with autism its onset and development and also to the encoded proteins of said genes associated with autism, its onset and development and the use of said genes, proteins or protein isoforms. The invention thus also relates to methods of screening for,...

20060194205 - Biochip with independent recognition areas and optical format and float scanning thereof: A biochip, and a device for reading the biochip, the biochip including a plurality of molecular recognition areas distributed with a determined layout to create a format of molecular recognition areas and a mechanism for making optical position marks for each molecular recognition area, distributed with a determined layout to...

20060194250 - Cancer diagnostic method based upon dna methylation differences: There is disclosed a cancer diagnostic method based upon DNA methylation differences at specific CpG sites. Specifically, the inventive method provides for a bisulfite treatment of DNA, followed by methylation-sensitive single nucleotide primer extension (Ms-SNuPE), for determination of strand-specific methylation status at cytosine residues....

20060194229 - Cancer markers and detection methods: The present invention relates to cancer markers and methods of detecting cancer markers in a sample. The sample may be peripheral blood. Cancer markers are most commonly mutated or abnormal DNA sequences associated with metastatic cancer. Markers may be detected using PCR, microarrays, or other nucleic acid or peptide-based assays....

20060194220 - Compositions and methods for detecting and treating tumors: In certain embodiments, this present disclosure provides compositions and methods for detecting EphB4 gene amplification in test cells. In certain embodiments, the present disclosure provides methods and compositions for evaluating tumor (cancer) status and prognosis in a subject having or suspected of having a tumor....

20060194240 - Compositions and methods of detecting an analyte by using a nucleic acid hybridization switch probe: Compositions are described for detecting binding of an analyte to a binding partner attached to a nucleic acid hybridization switch probe that includes first and second arm sequences and a support sequence that is at least partially complementary to both arm sequences, allowing the probe under hybridization conditions to form...

20060194224 - Computer-aided nucleic acid sequencing: A computer system for sequencing nucleic acids is provided. The computer system may be used for de novo sequencing of a nucleic acid sequence by analyzing the fluorescence intensities of hybridized nucleic acid probes on biological chips. The probes with the highest intensities are utilized to sequence the nucleic acid...

20060194206 - Diagnostics of diarrheagenic escherichia coli (dec) and shigella spp: A method for the identification of the diarrheagenic E. coli groups: ETEC (enterotoxigenic E. coli), A/EEC (attaching and effacing E. coli) EPEC (enteropathogenic E. coli), VTEC (verocytotoxin producing E. coli) and EIEC (enteroinvasive E. coli), and Shigella spp. is described. The bacterial identification is made possible by the specific detection...

20060194228 - Dna sequence recognition: To detect the presence of a match of a target DNA base sequence with a probe DNA base sequence, a single strand of the probe DNA base sequence is prepared. One end of the single strand probe DNA base sequence is linked to an electrode and the other end to...

20060194245 - Expression miniarrays and uses thereof: The present invention provides methods and devices for making new and inexpensive miniarrays suitable for gene expression analysis. Also provided herein are methods of diagnosis for specific tissue or condition using specialized diagnostic miniarrays that exhibit specific visual pattern as diagnostic readout....

20060194223 - Fast method for detecting micro-organisms in food samples: The present invention relates to a specific method accomplishing fast and specific identification of contaminating micro-organisms in large amounts of food stuffs. A method has been developed based on random genome fragments or Zipcode oligonucleotides and DNA microarray technology that overcomes the disadvantages of whole-genome DNA-DNA hybridisation. In particular, the...

20060194204 - Gene expressed in prostate cancer, methods and use thereof: A polypeptide is disclosed that is specifically detected in the cells of the prostate, termed Splice Variant-Novel Gene Expressed in Prostate (SV-NGEP). Polynucleotides encoding SV-NGEP are also disclosed, as are vectors including these poynucleotides. Host cells transformed with these polynucleotides are also disclosed. Antibodies and immunoconjugages are disclosed that specifically...

20060194230 - Genetic markers associated with benign prostatic hyperplasia: The present invention regards expression profiles of one or more nucleic acids indicative of the presence of, susceptibility to, and/or predicting response to therapy of benign prostatic hyperplasia (BPH) in an individual. The present invention identifies pathways not previously associated with BPH, therefore presenting novel diagnostic and therapeutic targets for...

20060194247 - Glycosylated specificity exchangers: The present invention is directed to ligand/receptor and antigen/antibody specificity exchangers comprising a saccharide or glycoconjugate. Methods of making these specificity exchangers and methods of using said specificity exchangers to treat or prevent human disease are described herein....

20060194227 - Heteroduplex tracking assay: A change in viral tropism occurs in many HIV positive individuals over time and may be indicated by a shift in coreceptor use from CCR5 to CXCR4. The shift in coreceptor use to CXCR4 has been shown to correlate with increased disease progression. In patients undergoing HAART, the predominant populations...

20060194200 - High throughput detection of glutathione s-transferase polymorphic alleles: A high-throughput assay for characterizing a subject's genetic makeup is disclosed. Specifically, a high-throughput assay utilizing PCR is disclosed that permits the rapid and accurate characterization of a subject's inherited alleles of the polymorphic glutathione S-transferase (GST) genes GSTM1, GSTM3, GSTP1, and GSTT1. This method allows detection of the specific...

20060194235 - Hlj1 gene expression: The human HLJ1 tumor suppressor gene is herein defined as regulated by promoter, enhancer, and silencer regions. HLJ1 promoter activity and gene expression are inversely correlated with metastatic ability. HLJ1 is highly expressed, and inducible, in cells with low metastatic ability and expressed to a lesser extent in highly metastatic...

20060194242 - Immunotherapy for immune suppressed patients: The present invention provides compositions of a natural cytokine mixture (NCM) for treating a cellular immunodeficiency characterized by T lymphocytopenia, one or more dendritic cell functional defects such as those associated with lymph node sinus histiocytosis, and/or one or more monocyte functional defects such as those associated with a negative...

20060194218 - Inducible fluorescence assay: In one aspect, the invention provides fluorescent probes and assays. The probes include a fluorophore-quencher pair that undergoes a switch from dark to fluorescent in response to a reaction of the quencher. The switch of the probe from dark to fluorescent is typically mediated by an enzyme that acts directly...

20060194207 - Kit for detecting nucleic acids: The present invention provides a reactor for carrying out in a single container the steps of the extraction of nucleic acids and the amplification of a nucleic acid, the procedures of the steps being usually different to each other. The reactor according to the present invention is a reactor for...

20060194241 - Lactobacillus specific probes: Provided herein are sequence primers and probe for the detection of Lactobacillus species as well as diagnostic kit thereof....

20060194233 - Ligand of the protein \"beacon\": The present invention relates generally to a ligand for a protein associated with modulating obesity, diabetes and metabolic energy levels in animals and humans and to genetic sequences encoding the ligand. More particularly, the present invention is directed to a ligand of the protein “beacon” and its homologues. The identification...

20060194209 - Method for controlled release of enzymatic reaction components: Compositions and reaction kits are provided for controlling an enzymatic process. The compositions and kits include a substantially water-insoluble, water-permeable polymer and a poorly water-soluble salt of an essential ionic enzymatic reactant. The disclosed compositions and methods are particularly useful for improving the specificity and performance of PCR....

20060194237 - Method for diagnosing peripheral neuropathy: Genes whose expression is correlated with the presence of CIDP or vasculitic neuropathy are disclosed. Probes and sets of nucleic acids and proteins specific for these genes are described, as are molecular and immunological methods for aiding in the diagnosis of these disease conditions in a subject....

20060194199 - Method for diagnosing testicular seminomas: Objective methods for detecting and diagnosing testicular seminoma (TS) arc described herein. In one embodiment, the diagnostic method involves the determining a expression level of TS -associated gene that discriminate between TS and nomal cell. The present invention further provides methods of screening for therapeutic agents useful in the treatment...

20060194208 - Method for the detection of cytosine methylations in dna with the aid of scorpion: The invention relates to a method for analyzing cytosine methylations in DNA sequences, according to which non-methylated cytosines are first converted into uracil while 5-methylcytosine remains unmodified. The DNA is then amplified by means of a polymerase and at least one primer whose 5 end is connected to a probe...

20060194202 - Method for the manufacture of nucleic acid molecules: The present invention is related to a method for the manufacture of a nucleic acid molecule comprising the steps of a) providing a first at least partially double-stranded oligonucleotide which has a modification allowing the oligonucleotide to be coupled to a surface, whereby the oligonucleotide comprises a recognition site for...

20060194217 - Method of genotyping and phenotyping hepatitis b viruses resistant to antiviral molecules: The invention provides a method of preparation of HBV genomic amplicons which makes it possible, using the same amplicon obtained from a patient, to evaluate HBV strains resistant to antiviral agents by analysing, their genotype, their phenotype and their replicative capacity. The method of the invention guarantees to obtain the...

20060194248 - Method of measuring human cyp3a inducibility: A method for measuring human CYP3A inducibility upon administration of a test drug, characterized in that a non-human animal to which a test drug is administered or a population of human cells cultured in a medium containing a test drug is infected with viruses (A) and (B); virus (A) being...

20060194216 - Methods and compositions for assessing nucleic acids and alleles: The present invention is directed to methods and compositions for evaluating allelic variations with improved quality controls, methods of preparing such compositions, and applications employing such compositions and methods. In particular, the present invention provides methods and compositions for reducing false positives and/or false negatives in nucleic acid measurements....

20060194214 - Methods for assembly of high fidelity synthetic polynucleotides: Disclosed are methods of manufacturing synthetic DNAs, that is, DNAs made at least in significant part by chemical synthesis of polynucleotide polymers. Also provided are methods for assembling plural DNAs in the same pool by multiplexed assembly of synthetic oligonucleotides. In exemplary embodiments, the methods involve pre-amplification of one or...

20060194236 - Methods for generating hypermutable microbes: Bacteria are manipulated to create desirable output traits using dominant negative alleles of mismatch repair proteins. Enhanced hypermutation is achieved by combination of mismatch repair deficiency and exogenously applied mutagens. Stable bacteria containing desirable output traits are obtained by restoring mismatch repair activity to the bacteria....

20060194243 - Methods for identifying dna copy number changes: Methods and computer software products for identifying changes in genomic DNA copy number are disclosed. Methods for identifying homozygous deletions and genetic amplifications are disclosed. Genomic DNA is amplified generically and amplified sample is hybridized to an expression array. The expression array comprises probes to regions of genes that are...

20060194234 - Methods for identifying small molecules that bind specific rna structural motifs: The present invention relates to a method for screening and identifying test compounds that bind to a preselected target ribonucleic acid (“RNA”). Direct, non-competitive binding assays are advantageously used to screen bead-based libraries of compounds for those that selectively bind to a preselected target RNA. Binding of target RNA molecules...

20060194211 - Methods for prognosis and treatment of solid tumors: Solid tumor prognosis genes, and methods, systems and equipment of using these genes for the prognosis and treatment of solid tumors. Prognosis genes for a solid tumor can be identified by the present invention. The expression profiles of these genes in peripheral blood mononuclear cells (PBMCs) are correlated with clinical...

20060194225 - Methods, compositions, and kits for forming self-complementary polynucleotides: The present teachings generally relate to methods, kits, and compositions for detecting target polynucleotide sequences. The teachings also relate to ligation and amplification reactions that generate self-complementary polynucleotide products. In some embodiments, ligation reactions are performed with probes that result in the formation a self-complementary ligation product. Ligation of a...

20060194215 - Methods, reagents and kits for reusing arrays: Methods for stripping and reusing arrays are disclosed. Also disclosed are reagents and kits comprising control target molecules for determining the efficacy of a stripping condition to which an array has been exposed....

20060194239 - Mitochondrial complex i mutations in parkinson's disease and aging: The present invention provides methods for diagnosing and treating Parkinson's disease based on mitochondrial mutations. The present invention also provides methods for diagnosing and treating other diseases and disorders based on mitochondrial mutations....

20060194226 - Modified cyanovirin protein: This disclosure relates to a modified version of cyanovirin-N protein and a transformed Lactobacillus bacterium that is capable of recombinantly expressing this modified protein. Further disclosed are polynucleotide sequence encoding the modified cyanovirin-N protein, expression cassette that allows the expression of the modified protein, and method of make and use...

20060194210 - Novel therapeutic targets in cancer: The present invention relates to novel sequences for use in detection, diagnosis and treatment of cancers, especially lymphomas. The invention provides cancer-associated (CA) polynucleotide sequences whose expression is associated with cancer. The present invention provides CA polypeptides associated with cancer that are present on the cell surface and present novel...

20060194232 - Nucleotide compositions and uses thereof: The present invention relates to preparation of nucleotide compositions and uses thereof for conducting nucleic acid analyses. The compositions and methods embodied in the present invention are particularly useful for nucleic acid analyses that require high-resolution detection of labeled nucleotides or labeled nucleic acid targets....

20060194203 - Packing cell system for eb virus vector: A packaging cell is constructed by preparing a packaging cell carrying an EB virus gene lacking a packaging signal but not carrying a wild type EB virus gene having the packaging signal, and introducing an amplicon plasmid having the packaging signal but having no viral replication ability into the cell....

20060194238 - Peripheral neuropathy diagnosis: Genes whose expression is correlated with the presence of CIDP or vasculitic neuropathy are disclosed. Probes and sets of nucleic acids and proteins specific for these genes are described, as are molecular and immunological methods for aiding in the diagnosis of these disease conditions in a subject....

20060194249 - Process for the detection of chromosomal aberrations in interphase nuclei: The present invention relates to a process for the detection of chromosomal aberrations using e.g. a high resolution multicolor-banding (MCB) technology....

20060194219 - Secreted and transmembrane polypeptides and nucleic acids encoding the same: The present invention is directed to novel polypeptides and to nucleic acid molecules encoding those polypeptides. Also provided herein are vectors and host cells comprising those nucleic acid sequences, chimeric polypeptide molecules comprising the polypeptides of the present invention fused to heterologous polypeptide sequences, antibodies which bind to the polypeptides...

20060194231 - Suspension arrays of cross-reactive oligonucleotide-based sensors: Cross-reactive arrays on encoded beads are used to correlate ‘fingerprints’ of urine, serum and other biological liquids to disease states. Fluorescent hydrophobic sensors are based on nucleic acid three-way junctions, and beads may be encoded by size (could be registered by light scattering) and fluorescence in combination with flow cytometry...

20060194213 - Synthesis of 3'-, or 5'-, or internal methacrylamido-modified oligonucleotides: New modifiers were synthesized for incorporation of a methacrylic function in 3′-, 5′- and internal positions of oligonucleotides during solid phase synthesis. A modifier was used for synthesis of 5′-methacrylated oligonucleotides for preparation of microarrays by a co-polymerization method....

20060194212 - Treatment of aids: The invention includes methods of treating HIV infection in a patient where the method includes administration of an antibody to TNF-alpha and an antibody to interferon-gamma to the patient and administering antiretroviral therapy to a patient. The invention further includes methods of treating HIV infection in a patient where the...

20060194221 - Treatment of herpes: The present invention includes methods for the treatment of a herpes simplex virus infection comprising the administration of inhibitors of gamma interferon, tumor necrosis alpha and interleukin-1....

20060194222 - Triplex probe compositions and methods for polynucleotide detection: The present invention provides composition and methods for the detection and measurement of target nucleic acids. The probes of the present invention, or triplex probes, comprise a complex of three oligonucleotide probes including: (1) a first oligonucleotide probe, (2) a second oligonucleotide probe, and (3) a bridging oligonucleotide probe. In...

20060194246 - Use of predetermined nucleotides having altered base pairing characteristics in the amplification of nucleic acid molecules: The present invention provides Improved methods for amplifying a nucleic acid molecule. More specifically, the invention provides methods for nucleic acid amplification which use primers having equivalent priming efficiency....

20060194244 - Uses of a chemokine receptor for inhibiting hiv-1 infection: This invention provides a polypeptide comprising a fragment of a chemokine receptor capable of inhibiting HIV-1 infection. In an embodiment, the chemokine receptor is C-C CKR-5. In another embodiment, the fragment comprises at least one extracellular domain of the chemokine receptor C-C CKR-5. This invention further provides different uses of...

20060194253 - A transfected cell line and a method using the same for the high throughput screening for inhibitors of the t-type calcium channel alpha-1h: The present invention relates to a cell line in which a potassium channel is introduced. More specifically, it is a cell line capable of activating the T-type α1H calcium channel by depolarization wherein a vector encoding a potassium channel is introduced. Since the cell line of the present invention is...

20060194256 - Compositions containing, methods involving, and uses of non-natural amino acids and polypeptides: Disclosed herein are non-natural amino acids and polypeptides that include at least one non-natural amino acid and methods for making such non-natural amino acids and polypeptides. The non-natural amino acids, by themselves or as a part of a polypeptide, can include a wide range of possible functionalities, but typical have...

20060194257 - Mecp2e1 gene: The invention is a novel MECP2E1 splice variant and its corresponding polypeptide. The invention also includes methods of using these nucleic acid sequences and proteins in medical diagnosis and treatment of neuropsychiatric disorders or development disorders....

20060194254 - Method for identifying optimal binding ligands to a receptor: The present invention provides a method for determining binding of a receptor to one or more ligands. The method consists of contacting a collective receptor variant population with one or more ligands and detecting binding of one or more ligands to the collective receptor variant population. The collective receptor variant...

20060194251 - Method of fixing low-molecular compound to solid-phase support: A method of fixing a low-molecular compound to a solid-phase support, which comprises a step in which a solution containing a low-molecular compound is brought into contact with a solid-phase support having a photoreactive compound bonded to the surface, a step in which the solution containing a low-molecular compound is...

20060194252 - Nanoscale patterning and immobilization of bio-molecules: A support for immobilizing target molecules comprises a substrate having a plurality of binding regions for binding select target molecules, with target-molecule-capturing agent immobilized at the binding regions. The binding regions are intersperse among other non-binding regions. The binding regions are of sub-micron size, have high selectivity and high binding...

20060194255 - Parallel patch clamp system: A system for high-throughput analysis of membranous samples having ion channels including at least one membranous sample, a multi-compartment structure including an extracellular chamber, an opposing intracellular chamber and a partition separating the extracellular and intracellular chambers, the partition having a plurality of apertures fluidly and electrically coupling the extracellular...

20060194258 - Polypeptide array synthesis: The present invention provides methods and apparatus for sequencing, fingerprinting and mapping biological macromolecules, typically biological polymers. The methods make use of a plurality of sequence specific recognition reagents which can also be used for classification of biological samples, and to characterize their sources....

20060194259 - Method for isolating an allosteric effector of a receptor: A method for isolating an allosteric effector of a receptor, by determining the variation of the dissociation kinetics of the complex formed between the receptor and one of its ligands in the presence of the allosteric effector, as compared to the kinetics dissociation, in the absence of the effector, and/or...

20060194261 - Methods for determining cellular response to stimuli: The present invention provides a method for determining cellular response to stimuli. The cells to be tested, for example, may be contained in a section of taste-bud containing lingual epithelium....

20060194260 - Screening assays for cannabinoid-ligand-type modulators of gpr55: Screening assays for compounds that modulate the interaction between cannabinoids and the GPR55 receptor are disclosed. Furthermore, a method for determining the selectivity of a test compound against a panel of cannabinoid receptors including GPR55 is disclosed....

20060194265 - Novel therapeutic targets in cancer: The present invention relates to novel sequences for use in detection, diagnosis and treatment of cancers, especially lymphomas. The invention provides cancer-associated (CA) polynucleotide sequences whose expression is associated with cancer. The present invention provides CA polypeptides associated with cancer that are present on the cell surface and present novel...

20060194266 - Use of protein rla-0 as a marker for colorectal cancer: The present invention relates to the diagnosis of colorectal cancer. It discloses the use of protein RLA-0 (60S acidic ribosomal protein P0) in the diagnosis of colorectal cancer. It relates to a method for diagnosis of colorectal cancer from a liquid sample, derived from an individual by measuring RLA-0 in...

20060194267 - Methods and kit for diagnosing tick borne illnesses: ELISA, Western Blot, and a peptide-based ELISA were applied to clinical specimens from patients with clinical symptoms of tick borne diseases, including Lyme disease. Peptides from different components of Borrelia during different cycles, including peptides from outer surface protein, leukocyte function associated antigens, immunodominant antigens, variable major proteins, and peptides...

20060194262 - P27 ubiquitination assay and methods of use: This invention relates to easy and reliable assays for the ubiquitination of p27. Because the assay can be performed as a plate capture assay, or as a homogenous time-resolved fluorescence resonance energy transfer assay, with defined, easily replicatable reaction conditions, it is particularly useful for high-throughput screening of, for example,...

20060194264 - Affinity based system for detecting particulates in a fluid: This invention provides methods and apparatus for detecting and quantifying particulate matter suspended in a fluid. Specifically, the invention provides an integrated, affinity-binding based, analytical system comprising a platform for performing an affinity-binding based assay for specifically binding particulates including microbial cells, and a detection means for detecting the particulates...

20060194263 - Small molecule mediated, heterogeneous, carbon nanotube biosensing: Nanosensors for detecting ananlytes and methods of detecting analytes have been developed in which a small molecule effector concentration is altered thereby causing changes in carbon nanotube conductance....

20060194268 - Method for measuring spinorphin: The present invention aims at providing a method for measuring spinorphin in a sample collected from a living body accurately with high sensitivity. The present invention provides a method for measuring spinorphin in a sample collected from a living body, comprising the steps of: (A) mixing a sample collected from...

20060194269 - Polysaccharide structure and sequence determination: The invention provides a method for the structural analysis of a saccharide, comprising: a) providing on a surface a plurality of essentially sequence- and/or site-specific binding agents; b) contacting said surface with a saccharide to be analyzed, or with a mixture comprising a plurality of fragments of said saccharide; c)...

20060194270 - Method for measuring oxidized ldl-crp complex and measurement kit: The present invention provides a method for measuring a “complex of oxidized LDL and CRP” which comprises using an “anti-CPR antibody”. An “anti-apoB antibody and/or anti-β2-GPI antibody” is preferably used in the method of the present invention, which preferably comprises at least a step for forming a sandwich complex represented...

20060194271 - Phosphorylation and regulation of akt/pkb by the rictor-mtor complex: In certain aspects, the invention relates to methods for identifying compounds which modulate Akt activity mediated by the rictor-mTOR complex and methods for treating or preventing a disorder that is associated with aberrant Akt activity....

20060194272 - Reference material for assaying d-dimer: The present invention relates to a reference material for assaying D-dimer, comprising a fibrin degradation product, wherein no less than 70% of a total peak area has the molecular weight of no less than 500,000 in a molecular weight distribution of the fibrin degradation product, measured by gel filtration chromatography....

20060194273 - Microfabricated apparatus for cell based assays: Apparatus and methods are provided for performing cell growth and cell based assays in a liquid medium. The apparatus comprises a base plate supporting a plurality of micro-channel elements, each micro-channel element comprising a cell growth chamber, an inlet channel and an outlet channel, a cover plate positioned over the...

20060194274 - Method for producing ketocarotinoids in genetically modified, non-human organisms: The present invention relates to a process for the preparation of ketocarotenoids by culturing genetically modified organisms which in comparison with the wild-type have a modified ketolase activity and a modified β-cyclase activity, to the genetically modified organisms, and to their use as foodstuffs and feedstuffs for the production of...

20060194286 - Apo-2li and apo-3 polypeptides: Novel polypeptides, designated Apo-3 and Apo-2LI, involved in apoptosis are provided. Compositions including chimeric molecules, nucleic acids, and antibodies are also provided....

20060194285 - Biological production of clavulanic acid and related compounds: Disruption of the two-component regulatory system encoded by the cbsA locus is associated with improved production of cephamycin C and/or clavulanic acid in Streptomyces clavuligerus....

20060194287 - Chemokine-binding protein and methods of use: Described herein are chemokine-binding domains of THAP-family polypeptides and pharmaceutical compositions which include a polypeptide comprising a chemokine-binding domain of a THAP-family polypeptide. Also disclosed are methods of binding chemokines, inhibiting the activity of chemokines, detecting chemokines, and reducing the symptoms associated with a chemokine mediated or influenced condition by...

20060194282 - Fluorescent protein variants and methods for making same: The present invention relates generally to variant fluorescent proteins, and more specifically to monomeric and dimeric forms of Anthozoan fluorescent proteins. In one aspect, the present invention provides variants of fluorescent proteins, where the variants have a reduced propensity to tetramerize, and form dimeric or monomeric structures. The invention also...

20060194277 - Method for producing target proteins by deleting or amplifyingibpa and/or ibpb gene coding for inclusion body-associated proteins: A method for producing target proteins by deleting or amplifying ibpA and/or ibpB genes coding for inclusion body-associated proteins. Two methods for producing target proteins using ibpA and/or ibpB genes coding for inclusion body-associated proteins of E. coli are described, The first method enhances the secretory production and activity of...

20060194289 - Method of production of recombinant proteins in eukaryote cells: The invention provides a method for production of polypeptides in eukaryote microcarrier cell culture, the method comprising the steps of (i) Culturing cells expressing said polypeptide on microcarriers under conditions and at a setpoint temperature appropriate for expression of said polypeptide; (ii) Cooling the culture to a predetermined temperature below...

20060194280 - Methods for refolding of recombinant antibodies: The present invention is generally directed to methods of producing an increase in the enrichment or recovery of preferred forms of IgG proteins. More particularly, the invention relates to subjecting preparations of such recombinant IgG proteins with a reduction/oxidation coupling reagent and optionally a chaotropic agent....

20060194281 - Novel hcv core‘protein, methods for diagnosis of hcv infections, prophylaxis, and for screening of anti-hcv agents: The present invention relates to a novel form of core+1 protein of Hepatitis C virus (HCV), designated shorter form core+1 protein. The shorter form core+1 protein of Hepatitis C virus is the product of translation of a coding sequence consisting of all or part of a nucleotide sequence extending from...

20060194288 - Nucleic acid molecules encoding endonucleases and methods of use thereof: We describe here an in vitro method of increasing complementarity in a heteroduplex polynucleotide sequence. The method uses annealing of opposite strands to form a polynucleotide duplex with mismatches. The heteroduplex polynucleotide is combined with an effective amount of enzymes having strand cleavage activity, 3′ to 5′ exonuclease activity, and...

20060194290 - Polypeptide variants with altered effector function: The present invention concerns polypeptides comprising a variant Fc region. More particularly, the present invention concerns Fc region-containing polypeptides that have altered effector function as a consequence of one or more amino acid modifications in the Fc region thereof....

20060194291 - Polypeptide variants with altered effector function: The present invention concerns polypeptides comprising a variant Fc region. More particularly, the present invention concerns Fc region-containing polypeptides that have altered effector function as a consequence of one or more amino acid modifications in the Fc region thereof....

20060194278 - Process for producing glucose dehydrogenase: A glucose dehydrogenase complex is produced by culturing an Escherichia bacterium, which is introduced with DNAs encoding the α-subunit and the β-subunit of glucose dehydrogenase of Burkhorderia cepacia in an expressible form, and in which expression of the ccm system (cytochrome c maturation system) is enhanced, to express the aforementioned...

20060194279 - Protein-refolding material: The present invention provides a method for refolding protein produced, for example, by Escherichia coli, that is inactive due to an as yet unformed higher order structure, or protein deactivated due to a change in conformation for some reason. The invention comprises a method, refolding kit, refolding agent, and molding...

20060194284 - Recombinant co-expression of vitamin k epoxide reductase subunit 1 to improve vitamin k dependent protein expression: The present invention relates to a host organism containing recombinant nucleic acids coding for the vitamin K reductase complex subunit 1 (VKORC1) and recombinant nucleic acids coding for a vitamin K dependent (VKD) protein, wherein both the recombinant VKORC1 and the recombinant VKD protein are expressed in said host organism....

20060194283 - Secreted and transmembrane polypeptides and nucleic acids encoding the same: The present invention is directed to novel polypeptides and to nucleic acid molecules encoding those polypeptides. Also provided herein are vectors and host cells comprising those nucleic acid sequences, chimeric polypeptide molecules comprising the polypeptides of the present invention fused to heterologous polypeptide sequences, antibodies which bind to the polypeptides...

20060194276 - Specificity grafting of a murine antibody onto a human framework: The current invention provides new human variable chain framework regions and humanized antibodies comprising the framework regions. The invention also provides a new method of identifying framework acceptor regions in framework sequences for backmutation to graft a donor sequence to the human framework....

20060194275 - Transporter and ion channels: The invention provides human transporters and ion channels (TRICH) and polynucleotides which identify and encode TRICH. The invention also provides expression vectors, host cells, antibodies, agonists, and antagonists. The invention also provides methods for diagnosing, treating, or preventing disorders associated with aberrant expression of TRICH....

20060194292 - Growth factor complexes and modulation of cell migration and growth: Isolated protein complexes are provided comprising growth factors such as IGF-I, IGF-II, VEGF or PDGF, or at least domains thereof that enable binding to and activation of both a growth factor receptor, and an integrin receptor-binding domain of vitronectin or fibronectin. These protein complexes may be in the form of...

20060194293 - Rb1 gene induced protein (rb1cc1) and gene: To provide a novel gene and protein involved in multidrug resistance in cancer, to elucidate functions of the gene and protein, to provide methods of detecting the gene and antibody against the protein and of testing and diagnosing cancer using the gene and antibody, we found a novel protein (RB1CC1)...

20060194294 - Process for the recovery of staurosporine from a fermentation broth: Staurosporine of the formula (I) is recovered from a fermentation broth in a process comprising an ultrafiltration and a diafiltration step....

20060194295 - Novel, esterase, fungus capable of producing the same and method for producing the same: The present invention provides a novel esterase derived from Ideonella sp. 0-0013 strain (FERM BP-08660) having the following properties: (1) function, substrate specificity: hydrolyzes methyl 3-hydroxypalmitate to generate 3-hydroxypalmitic acid and methanol; (2) optimal temperature for functioning: 37° C.; (3) optimal pH and stable pH range: pH 7 or more...

20060194296 - Ethanol production process: An improved ethanol production process providing novel stillage treatment is disclosed wherein the stillage is separated into four value added product streams that are subjected to drying conditions reducing or eliminating volatization of any VOC's in the product streams....

20060194297 - Process for the preparation of dihydroxy esters and derivatives thereof: wherein R and R′ represent optionally substituted hydrocarbyl groups and X represents a hydrocarbyl linking group. The process comprises either the stereoselective reduction of the keto group in a dihydroxy keto precursor followed by selective esterification of a primary hydroxy, or selective esterification of a primary hydroxy of a dihydroxy...

20060194299 - Method for recycling important nutritional elements from waste: The present invention relates to a process wherein waste material derived from human, animal and industrial areas is processed to utilize the energy resources present in the solid phase and, optionally, to recover important nutritional elements as well as toxic heavy metals. In particular, there is provided a process for...

20060194298 - Phytase produced from citrobacter braakii: The present invention relates to novel phytase enzyme, a gene coding the enzyme, and a Citrobacter sp. producing the enzyme. Particularly, the present invention relates to the phytase enzyme produced from Citrobacter sp. having (a) molecular weight of 47 kDa, (b) optimal pH of 3.5-4.5, (c) optimal temperature of 45-55°...

20060194300 - Process for preparing an alpha-1-antitrypsin solution: A process for preparing A1AT from A1AT-containing solutions, comprising the following steps: (a) subjecting an A1AT-containing solution to ion-exchange chromatography; (b) adding detergents and optionally a solvent for inactivating lipid-enveloped viruses; (c) followed by increasing the salt concentration to salt out the detergents. A1AT having a purity of >90% with...

20060194301 - Large-scale production of human serum butyrylcholinesterase as a bioscavenger: Disclosed herein are methods for the large-scale preparation of human butyrylcholinesterase (HuBChE) preparations from Cohn Fraction IV-4. As disclosed, the methods provide HuBChE preparations that are about 99% or more pure with recovery yields of about 60%. Also disclosed are the pharmacokinetics, safety and toxicity, stability and efficacy of the...

20060194302 - Method for producing amino acids by fermentation: The present invention provides a method for producing an amino acid selected from the group consisting of L-alanine, L-valine, L-leucine, L-isoleucine, L-methionine, L-phenylalanine, L-proline, glycine, L-serine, L-threonine, L-cysteine, L-tyrosine, L-asparagine, L-glutamine, L-lysine, L-histidine, L-arginine, L-aspartic acid and L-glutamic acid and useful as medicament, chemical agent, food material and feed additive...

20060194304 - Process for the depletion or removal of endotoxins: A process for the depletion or removal of endotoxins from preparations containing active ingredients designated for therapeutical use which are obtained from natural sources by genetic engineering and/or biotechnology by treatment with chromatographic material wherein said natural sources are lysed, the fractions obtained are optionally centrifuged, filtrated or treated with...

20060194303 - Wastewater treatment compositions: The present invention relates to a wastewater treatment composition and a process of decolorizing pulp and paper mill wastewater and, more specifically, to a process for treating wastewater effluent from a pulp or paper mill with a microorganism whereby color bodies in the pulp and paper wastewater are thereby removed...

20060194305 - Micro-holding pipette with an oblique opening: The present invention relates to a micro-holding pipette. The holding end of the micro-holding pipette is a oblique opening, i.e. the end surface of the opening of the holding end of the micropipette is at an acute angle with the ordinate axis of the holding end, which is less than...

20060194306 - System for gel electrophoretic immunoassay: A micro-analytical platform for performing electrophoresis-based immunoassays was developed by integrating photopolymerized cross-linked polyacrylamide gels within a microfluidic device. The microfluidic immunoassays are performed by gel electrophoretic separation and quantifying analyte concentration based upon conventional polyacrylamide gel electrophoresis (PAGE). To retain biological activity of proteins and maintain intact immune complexes,...

20060194307 - Cell measuring and sorting chip: The present invention provides a cell sorting device using an inexpensive chip capable of being exchanged for each sample for cell sorting. The cell sorting device uses flow paths formed on the substrate and has the following structure. The chip includes: a first flow path allowing a buffer fluid containing...

20060194308 - Imaging fluorescence signals using telecentricity: The present invention relates to the field of DNA analysis. In particular, the present invention is directed to a device for the parallel imaging of fluorescence intensities at a plurality of sites as a measure for DNA hybridization. More particular, the present invention is directed to a device to image...

20060194309 - Method and device for the formation of biological cell material: A method for producing cell material (20) having multiple biological cells (21), which have a predefined geometrical arrangement, includes the steps of providing a manipulation tool (10) having a tool body (11), whose surface (12, 14) at least partially contacts the cell material (20), and adjusting the manipulation tool (10)...

20060194310 - Cell culture spinner flasks: The present invention relates to culturing devices that are compact, utilize small amounts of cell culture media to establish and maintain cell cultures, and produce a large number of cells in a short period of time when compared to other cell culturing devices and techniques. Such devices are useful in...

20060194311 - Lna-cpg conjugates: The present invention relates to novel compositions comprising DNA attached to an immunostimulatory oligonucleotide (CpG) via a locked nucleic acid oligonucleotide of a defined sequence. In particular the present invention provides compositions comprising a plasmid containing a gene encoding a protein of interest, wherein said plasmid may be introduced to...

20060194312 - Method of cell culture observation, carbon substrate for cell culture observation, and method for manufacture thereof: The invention provides a substrate suitable for cell culture observation and a method of observation using the same. Crystalline carbon such as a graphite powder is mixed into a thermosetting resin such as a furan resin, and the mixture is molded in the shape of a sheet and carbonized to...

20060194314 - Novel bt toxin receptors from lepidopteran insects and methods of use: The invention relates to Bt toxin resistance management. The invention particularly relates to the isolation and characterization of nucleic acid and polypeptides for a novel Bt toxin receptor. The nucleic acid and polypeptides are useful in identifying and designing novel Bt toxin receptor ligands including novel insecticidal toxins....

20060194313 - Shear protectants in harvest microfiltration: The addition of buffer to a harvest stream during diafiltration can cause increased turbidity and have other undesirable effects including limiting product recovery. Methods and compositions related to the use of a non-ionic surfactant are provided for improving diafiltration....

20060194315 - Compositions and methods for the control, differentiaton and/or manipulation of pluripotent cells through a gamma-secretase signaling pathway: The current invention relates to the control and/or manipulation of the gamma-secretase signaling pathway in pluripotent cells to stabilize the cells in a pluripotent state and/or to control the differentiation of the pluripotent cells towards a differentiated state. The invention further includes feeder layers that contain or express ligands or...

20060194316 - Bone marrow-derived neuronal cells: Bone marrow stromal cells (BMSC) differentiate into neuron-like phenotypes in vitro and in vivo, engrafted into normal or denervated rat striatum. The BMSC did not remain localized to the site of the graft, but migrated throughout the brain and integrated into specific brain regions in various architectonic patterns. The most...

20060194317 - Expression of apoai and variants thereof using spliceosome mediated rna trans-splicing: Methods and compositions for generating novel nucleic acid molecules through targeted spliceosome mediated RNA trans-splicing that result in expression of a apoAI protein, an apoAI variant, the preferred embodiment referred to herein as the apoAI Milano variant, a pre-pro-apoAI or an analogue of apoAI. The methods and compositions include pre-trans-splicing...

20060194318 - Cultured cd14+ antigen presenting cells: The present invention provides isolated CD14+ antigen presenting cells, e.g., dendritic cells and isolated and enriched populations thereof as well as methods for isolation and enrichment. Also provided are methods for using the CD14+ antigenpresenting cells to modulate T cell responses in vivo, in vitro, and ex vivo....

20060194319 - Promoter substitution for immunoglobulin therapy: The present invention involves the identification of Bright as involved in immunoglobulin production, and the targeting of that function for the treatment of disease states associated with pathologic immunoglobulin production. Also provided are methods of identifying candidate substances with Bright-inhibitory activity....

20060194320 - Artificial immune organ: The invention relates to methods and devises for the faithful modeling of organ functions in vitro....

20060194321 - Directed differentiation of embryonic stem cells and uses thereof: The present invention provides methods for the directed differentiation of embryonic stem cells along the endodermal lineage, especially the pancreatic lineage....

20060194322 - Culture media compositions free of fetal bovine serum: A cell culture growth media free of Fetal Bovine Serum for use with parasitic organisms. The media includes calcium chloride, sodium bicarbonate, potassium chloride, sodium chloride, monosodium phosphate, glucose, hepes, ferric nitrate, magnesium sulfate, tricine, d-ribose, 2-deoxy ribose, adenosine-5-triphosphate (ATP), 2-deoxyadenylic acid (d-AMP), 5′-thymidylic acid (TMP), 2′-deoxyicitidine-5 monophosphate (d, 2′-deoxyuridine-5-monophosphate...

20060194323 - Defined systems for epithelial cell culture and use thereof: The present invention provides cell culture media formulations which support the in vitro cultivation of animal epithelial cells. The media comprise at least one fibroblast growth factor (FGF) and at least one agent that induces increased intracellular cAMP levels, and optionally comprise ascorbic acid. The present invention also provides methods...

20060188865 - Sample holding device and method for the production thereof: The invention relates to a sample holding device (100), particularly for cryopreserving at least one sample, which has a bundle (10) consisting of a multitude of tubular flexible sample compartments (11, 12,) and a retaining device (20), to which the bundle (10) of sample compartments is connected. The retaining device...

20060188866 - Diaminophenothiazine compositions and uses thereof: Cell senescence is delayed by contacting a cell specifically determined to be in need of delayed cell senescence with an effective amount of a diaminophenothiazine....

20060188867 - Method of cryopreserving cells and tissues by liposomal delivery of sugars to enhance post-thaw viability: A method for cryopreserving cells entails the liposomal delivery of intracellular sugar(s), such as trehalose, sucrose, raffinose, stachyose, and combinations thereof, into cells and tissues, such as red blood cells, for enhancing post-thaw viability. This method enables rapid and easy delivery of protective molecules into cells which thus greatly simplifies...

20060188868 - Direct detection method for products of cellular metabolism using tof-sims: A rapid and efficient method for novel biological substance screening by surface analysis has been developed using Time-of-Flight Secondary Ion Mass Spectrometry (ToF-SIMS). This method relies on the surface screening of an array of micro-organisms grown on porous membranes, which had previously been in contact with a solid growth medium....

20060188870 - Identification of active sites in enzymes: A method for determining the location of the active site of an enzyme is provided. The method comprising: determining a location of a reference point inside a functional unit of the enzyme; determining a limiting distance from the reference point; and identifying one or more molecular surface portions within the...

20060188869 - Method and apparatus for uv imaging: A method and apparatus for illuminating a subject with deep UV light and detecting 2-dimensional image information, e.g., a transmission image, fluorescence image, reflected light image, etc. The subject may be illuminated with a light pulse having a duration of 2 seconds or less and one or more components with...

20060188871 - Detection of very virulent infectious bursal disease virus: Methods of identifying animals infected with a vvIBDV are provided. The methods comprise contacting a nucleic acid sample obtained from the animal or a nucleic acid product obtained by amplifying RNA obtained from the animal with one or more probe pairs, each of which comprises a mutation probe and an...

20060188873 - Method for detecting a target polynucleotide: A method of detecting a target polynucleotide using a solution phase nucleic acid sandwich assay, wherein the solid support is an optical planar waveguide, and the label is detected by measuring the luminescence that is excited in the evanescent field of the waveguide-A fully automated system may be used. The...

20060188874 - Methods and compositions for colorimetrically assessing peptide characteristics: Methodologies and compositions useful for calorimetrically assessing peptide characteristics. By way of example, the methodologies can be used to qualitatively and quantitatively assess the degree to which a peptide sample incorporates beta sheet content. The colorimetric information also information is useful to predict solubility, formulating, and other peptide characteristics impacted...

20060188872 - Novel post-transcriptional regulatory elements and uses thereof: The invention provides a novel post-transcriptional regulatory element that can function as an RNA nucleo-cytoplasmic transport element. The invention also provides for an attenuated HIV-1 hybrid virus for use as a vaccine and a kit incorporating the hybrid virus. The kit also includes instructional material teaching the use of the...

20060188915 - Atropisomers of asymmetric xanthene fluorescent dyes and methods of dna sequencing and fragment analysis: Atropisomeric energy-transfer dye compounds are disclosed. A variety of molecular biology applications utilize atropisomeric xanthene fluorescent dyes as labels for substrates such as nucleotides, nucleosides, polynucleotides, polypeptides and carbohydrates. Methods include DNA sequencing, DNA fragment analysis, PCR, SNP analysis, oligonucleotide ligation, amplification, minisequencing, and primer extension....

20060188876 - Binding a target substance: Magnetic particles capable of binding a target substance, which comprise a magnetic material and a matrix material, wherein the magnetic material is remanent upon exposure to a magnetic field and the matrix material has a surface comprising functional groups which promote disaggregation of the particles in the presence of a...

20060188922 - Biopolymeric arrays having replicate elements: A method for designing an array is provided. In certain embodiments, this method includes grouping probes into a plurality of ranked groups of probes; and designing an array comprising the ranked groups of probes, wherein the array contains more replicates of probes in a higher ranked group as compared to...

20060188904 - Biosensors having single reactant components immobilized over single electrodes and methods of making and using thereof: Disclosed herein are single reactant components immobilized over single electrodes and methods of making and using thereof. Devices, such as biosensors, comprising the single reactant components immobilized over single electrodes are also disclosed. Assays using the single reactant components immobilized over single electrodes are disclosed as well as databases comprising...

20060188909 - Business methods for assessing nucleic acids: The present invention is directed to methods and compositions for evaluating nucleic acids, methods of preparing such compositions, and applications and business methods employing such compositions and methods. In particular, the present invention provides business methods for operating a gene expression measurement service....

20060188908 - Compositions and methods of use of standardized mixtures: The present invention is directed to methods and compositions for evaluating nucleic acids, methods of preparing such compositions, and applications and business methods employing such compositions and methods. In particular, the present invention provides business methods for operating a gene expression measurement service....

20060188910 - Compositions, methods, and kits for analyzing dna methylation: Compositions, methods, and kits for reducing strand amplification bias using bisulfite treated gDNA are provided. Methods for detecting and for quantitating the amplified bisulfite treated gDNA and inferring the presence, absence, and/or degree of methylation of target cytosine(s) in the gDNA are also provided. Such methods typically employ tailed first...

20060188927 - Compounds and processes for single-pot attachment of a label to nucleic acid: Compounds and methods are provided for a single-pot covalent attachment of a label to nucleic acids comprising forming a covalently attachable labeling reagent for alkylating the molecule. Then, combining the covalently attachable labeling reagent with a mixture containing the molecule, under conditions wherein the labeling reagent has reactivity with the...

20060188879 - Crh responsive genes in cns: The present invention relates generally to therapy and diagnosis of depression. In particular this invention relates to the polypeptides as well as to the polynucleotides encoding these polypeptides, wherein said polypeptides are shown to play a central role in mediating the endocrine response to corticotropin releasing hormone. These polypeptides and...

20060188917 - Device and method for multiple analyte detection: The invention is directed to a method and device for simultaneously testing a sample for the presence, absence, and/or amounts of one or more of a plurality of selected analytes. The invention includes, in one aspect, a device for detecting or quantitating a plurality of different analytes in a liquid...

20060188882 - Diagnostics and therapeutics for diseases associated with phosphodi-esterase 1c (pde1c): The invention provides a human PDE1C which is associated with the cardiovascular disorders, gastrointestinal and liver diseases, cancer disorders, neurological disorders, respiratory Diseases and urological disorders. The invention also provides assays for the identification of compounds useful in the treatment or prevention of cardiovascular disorders, gastrointestinal and liver diseases, cancer...

20060188881 - Dna amplification method and kit therefor: In a method for amplifying a DNA, comprising performing PCR using a sense primer and an antisense primer, PCR is performed in the presence of an additional sense primer comprising the sense primer and an oligodeoxyribonucleotide having a first additional sequence and ligated to the 5′ end of the sense...

20060188884 - Enhanced hiv-1 vaccines and methods for their use: The present invention provides peptides and proteins for use in second generation HIV vaccines and as diagnostic tools in the treatment and control of HIV infection. The antiviral protection shown by compositions of the present invention has not been previously achieved with an HLA epitope-enhanced vaccine. These findings define a...

20060188892 - Enzymatic digestion of tissue: The present invention concerns a compositions and method for isolating a nucleic acid from a cell-containing sample. There is disclosed a method comprising obtaining at least one cell-containing sample, which comprises a cell containing nucleic acid, obtaining at least one catabolic enzyme, obtaining at least one nuclease inhibitor, preparing an...

20060188925 - Feline ctla-4 nucleic acid and polypeptides: The present invention provides isolated and purified DNA encoding feline CD80 (B7-1) ligand, feline CD86 (B7-2) ligand, feline CD28 receptor, or feline CTLA-4 (CD 152) receptor, as well as vectors comprising nucleic acid encoding feline CD80, feline CD86, feline CD28, or feline CTLA-4. The present invention provides a host cells...

20060188877 - Fluorescence-quenching used to detect nucleic acid oligomer hybrization events at high salt concentrations: A method is described for detecting nucleic acid oligomer hybridization events by fluorescence quenching, which comprises as a first step the provision of a modified surface. The modification of the surface consists in the binding of at least one type of modified nucleic acid oligomers 201, wherein said nucleic acid...

20060188928 - Gene causative of rothmund-thomson syndrome and gene product: The RecQ4 helicase gene, belonging to the RecQ helicase gene family, is revealed herein to be the causative gene of Rothmund-Thomson syndrome. The present inventors found out that it is possible to diagnose Rothmund-Thomson syndrome by detecting mutation of this gene. Further, they uncovered that it is possible to treat...

20060188911 - Genetic testing method: A genetic testing method for specifically amplifying a DNA complementary to a target nucleic acid existing in a biologic sample, measuring the amplified DNA, and generating diagnosis support information on the basis of a measurement result is described that includes steps of: (a) specifically amplifying a DNA complementary to an...

20060188899 - High speed dna sequencer and method: This invention relates to a device for the determination of the sequence of nucleic acids and other polymeric or chain type molecules. Specifically, the device analyzes a sample prepared by incorporating fluorescent tags at the end of copies of varying lengths of the sample to be sequenced. The sample is...

20060188900 - High speed nucleic acid sequencing: The present invention is directed to a method of sequencing a target nucleic acid molecule having a plurality of bases. In its principle, the temporal order of base additions during the polymerization reaction is measured on a molecule of nucleic acid, i.e. the activity of a nucleic acid polymerizing enzyme...

20060188885 - High throughput functional genomic screening methods for osteoarthritis: High-throughput functional screening assays are provided that identify genes and gene products that are associated with the pathogenesis of osteoarthritis (OA) in chondrocytes. In addition, genes and gene products identified by such functional assays are also provided. The genes and gene products provided herein are useful inter alia for diagnosing...

20060188875 - Human genomic polymorphisms: The invention provides nucleic acid segments of the human genome including polymorphic sites, SNP haplotype blocks, SNP haplotype patterns for each block and informative SNPs for each pattern. Allele-specific primers and probes hybridizing to regions flanking these sites are also provided. The nucleic acids, primers and probes are used in...

20060188918 - Hybridization method and hybridization equipment: The equipment of the present invention can implement high-speed hybridization with a simple construction and is configured as described below. The equipment comprises a biochip prepared by fixing biopolymers onto a plurality of sites on a substrate, positive and negative electrodes for generating an electric field along the surface of...

20060188920 - Identification of compounds that inhibit replication of human immunodeficiency virus: The present invention relates to the discovery of a novel class of compounds that inhibit the replication of human immunodeficiency virus (HIV) and approaches to identify these compounds. More specifically, it has been found that enzymatically prepared alpha-hydroxyglycinamide and synthetically prepared alpha-hydroxyglycinamide inhibit the replication of HIV in human serum....

20060188903 - Implantation serine proteinases: This invention provides three novel serine proteinases that are important for female fertility, particularly in the process of hatching and implantation. These proteinases, as well as the nucleic acids, fragments, analogs, and/or inhibitors thereof, can be used to modulate hatching, implantation and female fertility in general....

20060188901 - Labelled nucleotides: Nucleosides and nucleotides are disclosed that are linked to detectable labels via a cleavable linker group....

20060188883 - Markers for colorectal cancer: Provided are previously uncharacterised markers of cancers, for example colorectal cancers, and uses of these as diagnostic and prognostic markers of cancers, and in particular colorectal cancers. The markers are SEQ ID NO: 1—hnRNP-K; SEQ ID NO:2—HMG-1; SEQ ID NO:3—proteasome subunit alpha type 1; SEQ ID NO:4—bifunctional purine biosynthesis protein;...

20060188898 - Markers for roscovitine: The present invention relates to pharmacodynamic markers for CDKIs including the candidate 2,6,9-tri-substituted purine known as roscovitine. The identity of these markers facilitates the convenient identification of roscovitine-like activity both in vitro and in vivo....

20060188887 - Method and system for elucidating the primary structure of biopolymers: The present invention relates to a method and to a system (100) for predicting the primary structure of biopolymers, especially of proteins and peptides, in which at least two algorithms or the results of at least two algorithms and/or of bioinformatic analyses are combined in order to increase the significance...

20060188880 - Method for detecting an analyte in a sample: The present invention relates to a method for detecting an analyte in a sample comprising the steps of providing detection probes being labeled with a first reporter, which detection probes are capable of binding to the analyte, providing a solid support, providing capture probes being bound or capable of binding...

20060188894 - Method for screening compounds against flaviviruses by using persistent virus-infected cell system: This invention provides a method for screening compounds against Flavivirus. More specifically, this invention provides a method for screening compounds against Flaviviruses by using a persistent virus-infected cell system, including (a) establishment of persistent virus-infected cell lines, (b) preparation of monoclonal antibody by using said persistent virus-infected cell lines, (c)...

20060188886 - Method of detecting or quantitatively determining mitochondrial dna 3243 variation, and kit therefor: A method for detecting a DNA having the mitochondrial DNA 3243 mutation, using quantitative PCR using a primer having a nucleotide sequence complementary to a nucleotide sequence starting from the nucleotide number 243 in the nucleotide sequence of SEQ ID NO: 2 and having a length of 12 to 30...

20060188888 - Method of screening for improved specific activity of enzymes: This invention relates to a method for screening libraries of enzyme variants for changes in specific activity by expression of a fusion protein consisting of at least two enzymes. By using one enzyme as a marker changes in specific activity for the other enzyme can be screened efficiently....

20060188891 - Methods and apparatus for controlling dna amplification: Apparatus and methods for optimizing DNA amplification are provided. The apparatus of the invention includes a DNA amplification cycler and a detector capable of measuring a parameter of at least one stage of DNA amplification that changes as the reaction of said stage approaches completion. It is presently preferred that...

20060188913 - Methods and products for enhancing immune responses using imidazoquinoline compounds: The invention involves administration of an imidazoquinoline agent in combination with another therapeutic agent. The combination of drugs may be administered in synergistic amounts or in various dosages or at various time schedules. The invention also relates to kits and compositions concerning the combination of drugs. The combinations can be...

20060188921 - Methods for diagnosing and treating systemic lupus erythematosus disease and compositions thereof: The present invention is directed to novel methods for diagnosis and prognosis of Systemic lupus erythematosus by identifying differentially expressed genes. Moreover, the present invention is also directed to methods that can be used to screen test compounds and therapies for the ability to inhibit systemic lupus erythematosus. Additionally, methods...

20060188878 - Methods to predict ederma as a side effect of drug treatment: This invention provides methods to predict the likelihood of occurrence of the side effect of edema in patients treated with a drug including, but not limited to, a TKI, such as Imatinib or GLEEVEC™/GLIVEC®. The methods employed use gene expression profile comparisons and the determination of specific SNPs and in...

20060188906 - Microfluidic chip for multiple bioassay and method of manufacturing the same: Provided are a microfluidic chip for multiple bioassay and a method of manufacturing the same. The method includes: forming microfluidic channels by coupling a channel structure having grooves for sample channels and probe channels in a bottom surface and a substrate; forming probe immobilization regions at intersections between the probe...

20060188923 - Novel human parvovirus b19 receptor and uses thereof: A novel receptor for human parvovirus B19, other than P antigen, as well as a reagent for measuring human parvovirus B19, a reagent for adsorbing human parvovirus B19, and an agent for suppressing infection, which utilize the receptor for human parvovirus B19 is disclosed. It was discovered that Ku 80...

20060188896 - Nucleic acid amplification with integrated multiplex dectection: A method mediated with in-vitro transcription (“IVT”) which permits miniaturization of multiplexed DNA and RNA analysis, and in which elongation-mediated multiplexed analysis of polymorphisms (eMAP®) is used as the analysis step, is described. Also described is a method mediated with IVT is for selecting a designated strand from T7-tagged double...

20060188926 - Nucleic acid sequences and methods for identifying compounds that affect rna/rna binding protein interactions and mrna functionality: Disclosed herein are nucleic acid sequences and their optimized subfragments which are located in the mRNA untranslated regions of therapeutically-relevant genes. These sequences specifically bind RNA binding proteins (RBPs) and/or regulate the mRNA functionality. Also disclosed are methods of optimizing a subfragment of a parent nucleic acid sequence such that...

20060188919 - Nucleic acid sequences having characteristics of enhanced expression in human neuroblastoma with favorable prognosis based on comparison between human neuroblastoma with favorable prognosis and human neuroblastoma with unfavorable prognosis: There are disclosed a nucleic acid which is derived from the gene expressed in human neuroblastoma, and which comprises any sequence selected from the group consisting of the nucleic acid sequences set forth SEQ ID NO:1 to NO:104 in the Sequence Listing, or its complementary nucleic acid; a fragment of...

20060188907 - Patterning method, substrate for biomolecule immobilization using the patterning method, and biochip employing the substrate: Provided is a linker functional group patterning method for biomolecule immobilization. The patterning method includes preparing a coating composition including a hydrophobic group-containing silane compound and a hydrophilic group-containing silane compound; forming a surface tension control layer by coating the coating composition on a substrate for biomoleucle immobilization; and forming...

20060188916 - Peptoids incorporating chemoselective functionalities: Molecules having potential biological activity, particularly peptoids, that are conjugated to solid phase supports, spacer groups, and/or ligation moieties, and methods of their preparation, are described. In some instances, the molecules of the invention are made entirely by solid phase synthesis. In other instances, the spacer groups are hydrophilic and...

20060188902 - Primer for nucleic acid detection: This application provides universal labeled primers for detection and amplification of nucleic acid molecules. These universal primers can be attached to the 5′-end of a target sequence-specific primer. In particular examples, the universal primer includes a labeled nucleotide flanked on both sides a nucleotide whose complement nucleotides changes a detectable...

20060188905 - Process: A process for the preparation of coated polymer particles containing superparamagnetic crystals, said process comprising reacting surface-functionalized, superparamagnetic crystal-containing polymer particles of diameter less than 0.5 μm with at least one polyisocyanate and at least one diol....

20060188895 - Rapid prognostic assay for malignancies treated with epidermal growth factor receptor: A molecular assay for determining the sensitivity or resistance of malignancies to chemotherapy prior to initiation of chemotherapy and which also allows for monitoring the therapeutic effects of the chemotherapy during treatment. The molecular assay measures tumor response to therapy with EGFR modulators and utilizes tumor mRNA as a starting...

20060188893 - Rolling circle amplification of rna: Disclosed are compositions and methods for amplification of RNA molecules. The disclosed method involves synthesizing first strand cDNA molecules from RNA molecules, circularizing the first strand cDNA molecules and replicating the circularized first strand cDNA molecules using rolling circle replication. The method can be aided by the use of specialized...

20060188912 - Sample preparation method incorporating an alkaline shock: Method of preparing a biological sample appropriate for use in a subsequent in vitro nucleic acid amplification reaction. The method involves a rapid, transient exposure to alkaline conditions which can be achieved by mixing an alkaline solution with a pH-buffered solution that includes a detergent and the biological sample to...

20060188914 - Secreted and transmembrane polypeptides and nucleic acids encoding the same: The present invention is directed to novel polypeptides and to nucleic acid molecules encoding those polypeptides. Also provided herein are vectors and host cells comprising those nucleic acid sequences, chimeric polypeptide molecules comprising the polypeptides of the present invention fused to heterologous polypeptide sequences, antibodies which bind to the polypeptides...

20060188924 - Tcl-1 gene and protein and related methods and compositions: The present invention relates to nucleotide sequences of TCL-1 genes and amino acid sequences of their encoded proteins, as well as derivatives and analogs thereof, and antibodies thereto. The TCL-1 gene sequence is preferentially expressed early in T and B lymphocyte differentiation. The present invention further relates to the use...

20060188897 - Transposon mediated differential hybridisation: A method for identifying an essential gene of an organism comprises: (i) providing a Library of transposon mutants of the said organism; (ii) isolating polynucleotide sequences from the library which flank inserted transposons; (iii) hybridising the said polynucleotide sequences with a polynucleotide library from the said organism; and (iv) identifying...

20060188890 - Ubiquitin-based protein interaction assays and related compositions: The invention provides, inter alia, methods for identifying substrates for E3 proteins that mediate ligation of ubiquitin or ubiquitin-like proteins....

20060188889 - Use of differentially expressed nucleic acid sequences as biomarkers for cancer: The present invention relates to novel marker sequences that are differentially expressed in cancer cells or tissue of a subject with cancerous conditions. The present invention also relates to assays for diagnosis, prognosis, staging, monitoring, therapeutic treatment, and marker sequence related agents including probes, primers, antibodies, and therapeutic compositions....

20060188936 - Alzheimer's related proteins and methods of use: Disclosed is a method for identifying substances that alter the interaction of a presenilin protein with a presenilin-binding protein, including contacting at least the interacting domain of a presenilin protein to a presenilin-binding protein in the presence of a test substance, and measuring the interaction of the presenilin protein and...

20060188943 - Color-encoding and in-situ interrogation of matrix-coupled chemical compounds: A method and apparatus for the physico-chemical encoding of a collection of beaded resin (“beads”) to determine the chemical identity of bead-anchored compounds by in-situ interrogation of individual beads. The present invention provides method and apparatus to implement color-coding strategies in applications and including the ultrahigh-throughput screening of bead-based combinatorial...

20060188940 - Combinatorial hydrogel formulation: The present invention concerns a method, apparatus (array assembly), and system for fabricating and formulating hydrogels using combinatorial techniques; a hydrogel array fabricated using the method, apparatus, and/or system of the invention; and methods and systems for testing the properties of arrayed hydrogels, such as bulk and interfacial mechanical properties....

20060188938 - Compounds for inhibiting beta-amyloid production and methods of identifying the compounds: Provided are compounds useful for treating diseases associated with a cerebral accumulation of Alzheimer's amyloid, such as Alzheimer's disease. Also provided are methods for screening for such compounds, by measuring capacitative calcium entry in cells which optionally overexpress APP or a fragment thereof. Also provided are methods of treating or...

20060188942 - Detection of micro-organisms: The present invention concerns methods for detecting the presence of a micro-organism in a fluid, gaseous and solid samples, together with apparatus for use in same. The invention comprises the use of a plurality of hollow fibres to filter the fluid and the capture of the micro-organisms on the membrane...

20060188939 - Fecal sample test device and methods of use: The present invention includes devices and methods for the detection of an analyte in a fecal sample. The fecal sample test device includes a sample collection structure and sample collection housing, a detection housing, a fecal suspension solution or fecal dilution solution, a detection housing capable of attachment to the...

20060188937 - G-protein estrogen membrane receptor: The present invention includes compositions and methods for the identification, isolation and characterization of a G-protein-coupled receptor that binds to an estrogen or estrogen-like molecule, to modulators of the receptor's activity and diagnostic methods for use in detection and treatment of a G-protein-coupled receptor related cancer....

20060188941 - High throughput screening assays utilizing affinity binding of green fluorescent protein: Novel methods of detecting fluorescent proteins are described. The methods result in vastly improved signal-to-noise ratios in assays measuring fluorescence of a fluorescent protein specifically by employing a unique trapping step to microconcentrate the fluorescent protein and by using improved optical techniques. The trapping step may be a chemical or...

20060188933 - Irak-m is a negative regulator of toll-like receptor signaling: Isolated nucleic acid encoding the amino acid sequence of murine IRAK-M; expression vectors comprising nucleic acid encoding IRAK-M and host cells comprising them are disclosed. IRAK-M is induced upon toll-like receptor (TLR) stimulation and negatively regulates TLR signaling. Methods for identifying antagonists and agonists of IRAK-M are described....

20060188930 - Isolated fluorescent protein from clytia gregaria cgfp and use thereof: The invention relates to the nucleotide and amino acid sequences and to the activity and use of the fluorescent protein CGFP....

20060188929 - Method capable of being automated for detection of prpres and uses thereof: This detection method uses a solid support whereon plasminogen is immobilized, and includes essentially the following steps: (a) preparing the biological sample during which the latter is incubated on a homogenizing buffer which comprises an ionic or nonionic surfactant, a glucose-containing buffer, a saccharose-based buffer and a PBS buffer or...

20060188931 - Method of preparing affinity ligands: The present invention is a method of preparing affinity ligands, which method comprises to provide a cyclic scaffold comprising a thiol group, a carbonyl group and an amine group; to derivatise the amine group of said scaffold with an electrophile that carries either a functionality capable of affinity interaction or...

20060188932 - Particle having magnetic material incorporated therein, process for producing the same, particle for immunoaasay and method of immunoassay: It is an object of the present invention to provide magnetic substance-encapsulated particles which have uniform magnetism, high dispersion stability and a narrow particle size distribution, a method of producing the same, particles for immunoassay formed by using the magnetic substance-encapsulated particles and a method of immunoassay in which the...

20060188935 - Protein and its use in diagnosing alzheimer's disease: Embodiments are directed to a peptide and protein containing that peptide, nucleic acids, antibodies, as well assays detecting the peptide or protein, and methods of identifying patients with or at risk of developing Alzheimer's Disease and other neurodegenerative diseases....

20060188934 - System and method for implementing a high-sensitivity sensor with improved stability: A high-sensitivity sensor with improved stability includes nanostructure-based sensors that are arranged such that a first nanostructure-based sensor (“shielded sensor”) is shielded from potential exposure to an environmental factor of interest and a second nanostructure-based sensor (“exposed sensor”) is allowed potential exposure to an environmental factor of interest. Further, all...

20060188944 - Methods of assaying receptor activity: Described are methods of detecting G-protein coupled receptor (GPCR) activity in vivo and in vitro; methods of assaying GPCR activity; and methods of screening for GPCR ligands, G Protein-coupled receptor kinase (GRK) activity, and compounds that interact with components of the GPCR regulatory process....

20060188946 - Nanobio device of imitative anatomy structure: objects to be achieved by the invention are to provide a nanobio device in which cultured cells are organized at a high-level in a state near in vivo, and to provide a method of using the nanobio device of imitative anatomy structure. The nanobio device of imitative anatomy structure of...

20060188945 - Screening methods: The present invention relates to methods for the identification of modulators of cytokine class I receptors by determining whether a compound that binds to a cytokine class I receptor at a site different from the binding site of the naturally-occurring cytokine ligand is effective at modulating the amount of the...

20060188948 - Methods for identifying agents that modulate apoptosis in cells that over-express a bcl-2 family member protein: The present invention provides methods and combinations of methods for identifying agents that modulate the apoptotic state of a cell by binding to the hydrophobic groove of a Bcl-2 family member anti-apoptotic protein. In certain embodiments, the methods generally comprise the use of Bcl-2 family member proteins having one or...

20060188949 - Use of protein plst as a marker for colorectal cancer: The present invention relates to the diagnosis of colorectal cancer. It discloses the use of protein PLST (T-plastin) in the diagnosis of colorectal cancer. It relates to a method for diagnosis of colorectal cancer from a liquid sample, derived from an individual by measuring PLST in said sample. Measurement of...

20060188950 - Use of protein spee as a marker for colorectal cancer: The present invention relates to the diagnosis of colorectal cancer. It discloses the use of protein SPEE (sperrnidine synthase) in the diagnosis of colorectal cancer. It relates to a method for diagnosis of colorectal cancer from a liquid sample, derived from an individual by measuring SPEE in said sample. Measurement...

20060188947 - Reagent for producing a protein chip: An object of the present invention is to provide a reagent for protein synthesis by a simple operation which is advantageous in a cell-free protein synthesis in many samples in a test using an aimed (specific) protein or a high throughput analysis using a protein chip, etc. and also to...

20060188951 - Method for measuring the level of anti-beta-amyloid antibody in body fluids and diagnostic kit for alzheimer's disease using same: A method for measuring a concentration of a β-amyloid antibody in a body fluid sample through a reaction forming an antigen-antibody complex uses an antigen protein binding specifically to the β-amyloid antibody existing in a body fluid of an Alzheimer's disease patient. A diagnostic kit for Alzheimer's disease using the...

20060188952 - Homogeneous fluorescence assay for kinases, phosphatases, and phosphodiesterases: The present invention relates to a homogeneous assay method of quantitative measurement of kinase, phosphatase and phosphodiesterase (PDE) reactions. The method may be used both in a direct and in a competitive assay format....

20060188953 - Methods and compositions that affect melanogenesis: The invention provides methods of screening for compounds that affect melanogenesis and the function of P protein in organisms, cells, or cell-free systems. The invention further relates to pharmacologic and cosmetic uses of methods of inhibiting melanogenesis, methods of activating melanogenesis, and compounds and pharmacologic compositions useful for the inhibition...

20060188954 - Method and apparatus for automated reprocessing of tissue samples: A method and apparatus of automatically reprocessing a specimen for microscopic examination is disclosed. Processing of a specimen for microscopic examination involves fixation of the specimen and preparation of the embedded specimen from the fixed specimen. There are instances where, once a specimen has been processed, it is necessary to...

20060188955 - Gene targets for enhanced carotenoid production: The present invention provides genetically manipulated cells and methods for utilizing same. Methods of enhanced carotenoid synthesis utilizing cells genetically disrupted for a yjiD, hnr or yjfP gene, or further disrupted for a gdhA, gpmB aceE, ppc, talB or fdhF gene, or any combination thereof, or cells inhibited for their...

20060188962 - 19 human secreted proteins: The present invention relates to novel human secreted proteins and isolated nucleic acids containing the coding regions of the genes encoding such proteins. Also provided are vectors, host cells, antibodies, and recombinant methods for producing human secreted proteins. The invention further relates to diagnostic and therapeutic methods useful for diagnosing...

20060188958 - Artificial enzyme and method for producing the same: The method for producing an artificial enzyme according to the invention is a method for producing an artificial enzyme and includes the step of selecting an artificial enzyme precursor which includes an oligonucleotide sequence containing modified nucleosides prepared by introducing a substituent into each nucleoside and at least one of...

20060188960 - Chimeric histamine h3 receptor: Chimeric H3 receptor polypeptides are provided, as are polynucleotides encoding such polypeptides, and vectors and cells for recombinant expression of such polypeptides. Chimeric H3 receptor polynucleotides and polypeptides may be used, for example, to identify agents that specifically interact with H3 receptor. Such agents find use within therapies for humans...

20060188959 - Crystal structure of worm nitfhit reveals that a nit tetramer binds tow fhit dimers: In invertebrates, Fhit is encoded as a fusion protein with Nit. Outside of invertebrates, Nit homologs are found as separate polypeptides in organisms with Fhit homologs. Therefore, Nit and Fhit are expected to interact physically and function in the same cellular pathway. The structure of the NitFhit fusion protein and...

20060188961 - Intergenic regions as novel sites for insertion of hiv dna sequences in the genome of modified vaccinia virus ankara: The invention relates to novel insertion sites useful for the integration of HIV DNA sequences into the MVA genome, and to the resulting recombinant MVA derivatives....

20060188957 - Novel tnf receptor death domain ligand proteins and inhibitors of ligand binding: Novel TNF receptor death domain (“TNF-R1-DD”) ligand proteins are disclosed. Polynucleotides encoding the TNF-R1-DD ligand protein are also disclosed, along with vectors, host cells, and methods of making the TNF-R1-DD ligand protein. Pharmaceutical compositions containing the TNF-R1-DD ligand protein, methods of treating inflammatory conditions, and methods of inhibiting TNF-R death...

20060188956 - Relaxin3-gpcr135 complexes and their production and use: A GPCR135/relaxin3 complex is described, which is useful as an assay reagent for screening for modulators of GPCR135 receptor activity....

20060188963 - Collectin-complement activating protein chimeras: The present invention relates to a fusion protein capable of activating the complement system, the fusion protein comprising a first polypeptide sequence derived from a lectin-complement pathway activating protein or a functional homologue thereof; and a second polypeptide sequence derived from a collectin or a functional homologue thereof; wherein said...

20060188964 - Processing for producing and crystallizing g-protein coupled receptors: This invention provides methods for producing a membrane-bound protein in mammalian cells. This invention also provides nucleic acids for making novel fusion proteins (e.g., GPCR fusion proteins). This invention further provides related bacterial expression vectors; expression methods; fusion proteins; bacterial cells; GPCR vector screens; bacterial spheroplasts; methods for making anti-GPCR...

20060188965 - Removal of minerals from cellulosic biomass: Disclosed is a method for removing minerals from a cellulosic biomass. For example, the biomass may be prewashed with an acid solution and rinsed with water to remove minerals prior to acid saccharification. The removal of minerals may reduce overall acid requirements, and decrease pretreatment costs....

20060188966 - Natural, chimeric and hybrid glycosaminoglycan polymers and methods of making and using same: The present invention relates to methodology for polymer grafting by a polysaccharide synthase and, more particularly, polymer grafting using the hyaluronate or chondroitin or heparin/heparosan synthases from Pasteurella multocida, in order to create a variety of glycosaminoglycan oligosaccharides having a natural or chimeric or hybrid sugar structure....

20060188967 - Method for extracting dna from organisms: A method for the indirect extraction of DNA greater than 300 kb in size from non-cultivatable organisms contained in an environmental sample is disclosed. The method comprises isolating the organisms from the sample and embedding the isolated organisms in a block of agarose where the organisms are subsequently lysed and...

20060188968 - Process for production of ethanol using stable yeast crystals in modified conventional batch reactor: Ethanol demand is ever increasing mainly because of its utilization as organic solvent and as starting compound for production of many organic solvents. In addition, it can replace the natural petroleum sources as energy fuels. Conventional method of ethanol production is performed mainly by batch fermentation, which requires addition of...

20060188969 - Process for the heterotrophic production of microbial products with high concentrations of omega-3 highly unsaturated fatty acids: A process for the heterotrophic or predominantly heterotrophic production of whole-celled or extracted microbial products with a high concentration of omega-3 highly unsaturated fatty acids, producible in a aerobic culture under controlled conditions using biologically pure cultures of heterotrophic single-celled fungi microorganisms of the order Thraustochytriales. The harvested whole-cell microbial...

20060188970 - High pressure refolding of protein aggregates and inclusion bodies: The present disclosure provides an effective method for the refolding of denatured proteins in solution so that properly folded, biologically active protein in solution is recovered in high yield. The refolding takes place at pressures between about 0.25 kbar to about 3.5 kbar, advantageously at about 1.5 kbar to about...

20060188971 - Urate oxidase: The present invention relates, in general, to urate oxidase (uricase) proteins and nucleic acid molecules encoding same. In particular, the invention relates to uricase proteins which are particularly useful as, for example, intermediates for making improved modified uricase proteins with reduced immunogenicity and increased bioavailability....

20060188973 - 3'-phosphoadenosine-5'-phosphosulfate synthetase 1 (papss1) sequence variants: Isolated PAPSS1 nucleic acid molecules that include a nucleotide sequence variant and nucleotides flanking the sequence variant are described, as well as PAPSS1 allozymes. Methods for determining if a mammal is predisposed to joint disease or cancer also are described....

20060188972 - Targeted modification of chromatin structure: Methods and compositions for targeted modification of chromatin structure, within a region of interest in cellular chromatin, are provided. Such methods and compositions are useful for facilitating processes such as, for example, transcription and recombination, that require access of exogenous molecules to chromosomal DNA sequences....

20060188974 - Human protein kinases and protein kinase-like enzymes: t

20060188975 - Genetically modified somatic cells for sustained secretion of lysosomal proenzymes deficient in lysosomal storage disorders: The invention relates to various methods of treating lysosomal storage disorders using somatic cells and methods of delivering therapeutic enzymes. More particularly, gain-of-function or loss-of-function mutations in components of the intracellular Golgi to lysosome sorting pathway are used to enhance secretion of one or more lysosomal enzymes in somatic cells,...

20060188976 - Mutant protein having the peptide-synthesizing activity: The present invention is to provide an excellent peptide-synthesizing protein and a method for efficiently producing a peptide. A peptide is synthesized by reacting an amine component and a carboxy component in the presence of at least one of proteins shown in the following (I) and (II): (I) The mutant...

20060188977 - Non-tumorigenic mdck cell line for propagating viruses: The present invention provides novel MDCK-derived adherent non-tumorigenic cell lines that can be grown in the presence or absence of serum. The cell lines of the present invention are useful for the production of vaccine material (e.g., viruses). More specifically, the cell lines of the present invention are useful for...

20060188978 - Products and processes for waste control: Products and methods for controlling animal wastes are disclosed. The methods include making an inoculum containing a mixture of Bacillus species; mixing the inoculum with animal wastes and then incubating the mixture to result in an enriched culture; and then applying the enriched culture to the animal wastes. The inoculum...

20060188979 - Method and apparatus for improved temperature control in microfluidic devices: A microfluidic system and method for employing it to control fluid temperatures of fluids residing within microchannels of a microfluidic device. The microfluidic device is provided with a top layer and a bottom layer and microchannels configured therebetween. Temperature of the fluid within the microchannels is controlled in various ways...

20060188980 - System and method for processing biomass: According to one embodiment of the invention, a system for processing biomass comprises a chamber, a biomass input device, a fluid input device, and a retrieval device. The chamber is defined by at least a bottom, at least one wall, and a cover supported by the at least one wall....

20060188981 - Mn gene and protein: Identified herein is the location of the MN protein binding site, and MN proteins/polypeptides that compete for attachment to vertebrate cells with immobilized MN protein. Such MN proteins/polypeptides prevent cell-cell adhesion and the formation of intercellular contacts. The MN protein binding site is a therapeutic target that can be blocked...

20060188982 - Multiplexed analysis for determining a serodiagnosis of viral infection: Clinical samples can be analyzed using microparticles to determine the serodiagnosis of a viral infection from two candidate viral infections of the same viral group. Serodiagnosis can be determined via a pooled population of subsets of microparticles, with the particles in the pooled population having a bound viral group-reactive antibody...

20060188984 - Method of providing readily available cellular material derived from cord blood, and a composition thereof: The present invention is directed to the TVEMF-expansion of mammalian cord blood stem cells, preferably CD34+/CD38− cells, to compositions resulting from the TVEMF-expanded cells, and to a method of treating disease or repairing tissue with the compositions. Various benefits and advantages to the compositions of the present invention are discussed...

20060188983 - Postpartum-derived cells for use in treatment of disease of the heart and circulatory system: Cells derived from postpartum tissue are disclosed along with methods for their therapeutic use in diseases of the heart or circulatory system are disclosed. Cells may be used therapeuticall in either differentiated or undifferentiated forms, in homogenous cultures, or as populations with other cells, and in conjunction with other bioactive...

20060188985 - Use of adipose tissue-derived stromal cells for chondrocyte differentiation and cartilage repair: Methods and compositions for directing adipose-derived stromal cells cultivated in vitro to differentiate into cells of the chondrocyte lineage are disclosed. The invention further provides a variety of chondroinductive agents which can be used singly or in combination with other nutrient components to induce chondrogenesis in adipose-derived stromal cells either...

20060188989 - Inhibition of e3-ubiquitin ligase hakai for treatment of proliferative disorders: Human HAKAI (hsHAKAI), an E3-ubiquitin ligase, can be inhibited to treat proliferative disorders, such as cancers, dysplasias and hyperplasias. Effective levels of hsHAKAI can be inhibited, for example, using antisense oligonucleotides, ribozymes, interference RNA, and antibodies. Test compounds can be screened for binding to hsHAKAI, for disruption of hsHAKAI-E-cadherin binding,...

20060188988 - Optimized high yield synthetic plasmids: One aspect of the current invention is an optimized synthetic mammalian expression plasmid with a mutated origin of replication (e.g. “mut” family of plasmids). This new plasmid comprises a therapeutic element, and a replication element. The therapeutic element of the new plasmid comprises a eukaryotic promoter; a 5′ untranslated region...

20060188986 - Restoration of methylation states in cells: A method for altering a characteristic or state of a cell, or reprogramming a cell, comprising treating a first cell type with an agent capable of altering a characteristic or state in a cell or reprogramming a cell, and determining the degree of alteration of the treated cell by measuring...

20060188987 - Targeted deletion of cellular dna sequences: Disclosed herein are methods and compositions for targeted deletion of double-stranded DNA. The compositions include fusion proteins comprising a cleavage domain (or cleavage half-domain) and an engineered zinc finger domain, and polynucleotides encoding same. Methods for targeted deletion include introduction of such fusion proteins, or polynucleotides encoding same, into a...

20060188991 - Flexible vaccine assembly and vaccine delivery platform: Herein-described are various methods for making a vaccine that are made of re-assembled virus like particles (VLP). First, the VLPs are disassembled into encapsidation intermediate populations. Each encapsidation intermediate population undergoes, for instance, chemical conjugation of unique peptide or nucleic moieties to form separate populations. Thereafter, a predetermined amount of...

20060188990 - Novel prostate tumor-specific promoter: This invention provides novel transcriptional regulatory elements from the human TRPM4 (Transient Receptor Potential-Melastatin 4) gene. These promoter and enhancer elements preferentially activate transcription in prostate tumor cells and tissues as compared to other tissues. Methods and compositions are provided to employ TRPM4 promoter elements for prostate tumor-specific expression of...

20060188992 - Electroporation method including the use of depressurization/pressurization: An object of the present invention is to establish a transformation method simpler and more rapid than conventional techniques. This object can be solved by a method comprising the steps of a) holding a cell under a pressure different from an atmospheric pressure, and b) placing the cell and a...

20060188993 - Method of truncating both ends of a large piece of dna: A method of truncating both ends of a DNA insert flanked by two different loxP sequences using transposons carrying corresponding loxP sequences pertaining to the two ends...

20060183102 - Serum-free reagents for the isolation, cultivation, and cryopreservation of postnatal pluripotent epiblast-like stem cells: Serum-free solutions suitable for use with postnatal pluripotent epiblast-like stem cells are disclosed. Methods of using serum-free solutions are also disclosed....

20060183103 - Method for enriching adherent monocyte populations: The present invention provides methods and apparatus for the rapid and efficient collection and purification of activated monocytes. The methods and apparatus of the present invention provide means for effecting the collection and purification in an aseptic environment. The method involves filtering a blood component mixture through a monocyte-adhering filter;...

20060183104 - Cellular models of neuron-associated disorders and uses thereof: The present invention describes two new cell lines derived from embryonic stem cells and useful for analyzing and studying neuron-associated disorders. The present invention further relates to methods of analyzing stem cell differentiation, and methods of identifying new therapies for neuron-associated diseases...

20060183105 - Human brain-derived tissue-specific potassium channel: A novel human brain-derived potassium channel polypeptide is described which is developmentally regulated and tissue-specific to differentiated neurons. A full length cDNA which encodes the novel potassium channel polypeptide is disclosed as well as the interior structural region and the amino acid residue sequence of the human brain potassium channel...

20060183109 - Cleavage of nucleic acids: The present invention relates to means for cleaving a nucleic acid cleavage structure in a site-specific manner. Enzymes, including 5′ nucleases and 3′ exonucleases, are used to detect and identify nucleic acids derived from microorganisms. Methods are provided which allow for the detection and identification of bacterial and viral pathogens...

20060183113 - Infectious cdna clone of north american porcine reproductive and respiratory syndrome (prrs) virus and uses thereof: The invention provides isolated polynucleotide molecules, including plasmids; viral vectors; and transfected host cells that comprise a DNA sequence encoding an infectious RNA sequence encoding a North American PRRS virus; and also North American PRRS viruses encoded thereby. The invention further provides isolated infectious RNA molecules encoding a North American...

20060183107 - Method for diagnosis and monitoring of pathogenic infection by analysis of pathogenic transrenal nucleic acids in urine: The present invention relates to a method for diagnosing and/or monitoring a bacterial or parasitic infection by detection and quantification of the transrenal nucleic acids, derived from bacterial pathogenic agents or from parasites, in urine. The detection method optionally includes the isolation and the purification of the nucleic acids from...

20060183108 - Method for diagnosis and monitoring of viral infection by analysis of viral transrenal nucleic acids in urine: The present invention relates to methods for diagnosis or monitoring of viral infection by detecting the presence of transrenal viral nucleic acids or nucleic acids of viral origin in urine sample, with or without isolation of nucleic acids from a urine sample. The analysis of the nucleic acids is performed...

20060183112 - Method of separating biomolecules using nanopore: Provided is a method of separating particles, the method comprising: forming a first chamber and a second chamber separated by an interface with a pore, wherein the first and second chambers have electrodes with different polarities; placing particles to which a target biomolecule is bound from particles to which the...

20060183110 - Methods of evaluating cell surface receptor binding of a patient derived population of viral envelope protein constructs: The invention provides a method for identifying whether a compound inhibits entry of a virus into a cell which comprises: (a) obtaining nucleic acid encoding a viral envelope protein from a patient infected by the virus; (b) co-transfecting into a first cell (i) the nucleic acid of step (a), and...

20060183111 - Primary human hepatocytes for native hepatitis c virus (hcv) replication: Novel methods are disclosed for the long-term culture of human primary hepatocytes in tissue culture system. This culture system supports the replication, gene expression and dissemination of a multitude of pathogenic agents that reside in, or pass through, the liver. Among these, the invention advantageously provides for efficient hepatitis C...

20060183106 - Processes for identifying quadruplex-targeted antiviral molecules: Featured herein are processes useful for identifying candidate molecules that interact with a quadruplex structure formed in the central flap nucleic acid of retroviruses. The candidate molecules identified by the process may be used as antiviral agents....

20060183115 - Assays for dna methylation changes: A new technique to determine the extent of DNA methylation entails generating DNA fragments of a test sample by cleaving at methylation sites that are not methylated while sparing methylation sites in the DNA that are methylated. This approach provides enhanced sensitivity to differences of even a single methylated cytosine,...

20060183140 - Cationic conjugated polymers suitable for strand-specific polynucleotide detection in homogeneous and solid state assays: The invention further relates to polycationic multichromophores, which may be conjugated polymers, and methods, articles and compositions employing them as described herein. In some aspects, the invention relates to methods, articles and compositions for the detection and analysis of biomolecules in a sample. Provided assays include those determining the presence...

20060183129 - Characterization of the i-spomi endonuclease from fission yeast: Isolated DNAs encoding the enzyme I-SpomI and its recognition and cutting site are provided. The DNA sequences can be incorporated in cloning and expression vectors, transformed cell lines and transgenic animals. The vectors are useful in gene mapping and site-directed insertion of genes....

20060183121 - Chimeric autoprocessing polypeptides and uses thereof: A chimeric polypeptide comprising an autoprocessing segment having an amino acid sequence being capable of auto-cleavage, a polynucleotide encoding such a polypeptide, and uses of such a polypeptide and such a polynucleotide are provided....

20060183149 - Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays: The present invention describes a method for identifying one or more of a plurality of sequences differing by one or more single base changes, insertions, deletions, or translocations in a plurality of target nucleotide sequences. The method includes a ligation phase, a capture phase, and a detection phase. The ligation...

20060183151 - Device and method for multiple analyte detection: The invention is directed to a method and device for simultaneously testing a sample for the presence, absence, and/or amounts of one or more of a plurality of selected analytes. The invention includes, in one aspect, a device for detecting or quantitating a plurality of different analytes in a liquid...

20060183125 - Diagnostic and prognostic compounds and method: The present invention relates to a method of diagnosing bacterial or fungal sepsis in a subject, which method comprises the step of measuring the level of TREM-1-Ligand or TREM-1-Ligand nucleic acid in a biological sample obtained from said subject. The method can involve contacting said biological sample with a compound...

20060183122 - Fanc gene mutations in cancer: The present invention relates to methods of determining if a patient has cancer or is at increased risk of developing cancer, particularly pancreatic cancer, the method comprising testing a FANC gene for the presence of a cancer-associated coding change, wherein said presence of one or more cancer-associated coding changes is...

20060183141 - Gene expression signature for prediction of human cancer progression: Methods are provided for classification of cancers by the expression of a set of genes referred to as the core serum response (CSR), or a subset thereof. The expression pattern of the CSR in normal tissues correlates with that seen in quiescent fibroblasts cultured in the absence of serum, while...

20060183146 - Genetic markers in the hla-c gene associated with an adverse hematological response to drugs: Genetic markers in the HLA-C gene associated with adverse hematological response to drug therapy are disclosed. Compositions and methods for detecting and using these HLA-C markers in a variety of clinical applications are disclosed. Such applications include methods for testing an individual for susceptibility for an adverse hematological response, methods...

20060183143 - Method and apparatus for classifying nucleic acid responses to infectious agents: In one embodiment, the present invention is a method and apparatus for classifying nucleic acid responses to infectious agents. In one embodiment, a method for selecting genes to be analyzed to determine exposure to a condition (from among a plurality of potential conditions) includes determining, for each gene in a...

20060183119 - Method for determining predisposition to a physiological reaction in a patient: The present invention relates to a method for determining predisposition to a physiological reaction in a patient. Particularly, the present invention relates to a method for determining a predisposition to toxicity induced by a camptothecin analog or to an immunosuppressive mycophenolic acid-based therapy. This method comprises the characterization of nucleic...

20060183136 - Method for haplotyping and genotyping by melting curve analysis of hybridization probes: The present invention is directed to nucleic acid probes, complexes and methods of using such probes and complexes for molecular haplotyping and genotyping of mutations, using melting curve analysis of nucleic acid probes to discriminate between and determine the identity of multiple alleles at one or more loci....

20060183126 - Method for the detection of biomolecules based on the replication of bound nucleic acids: A biomolecules detection method, where the biomolecules to be detected are coupled to a first substance which is part of a nucleic acid replicating device, the formed biomolecule/substance complexes being bound to the solid phase bound binding molecule specific to the particular biomolecule, and, if called for, the non-bound biomolecule/substance...

20060183138 - Method of detecting the presence of absence of mixed varieties in grains, and identifying the mixed varieties: Provided is a DNA-level grain variety discrimination method of detecting the presence or absence of any other varieties of grains in object grains of a certain variety through multiplex PCR that uses the DNAs extracted from the grains or from their processed products as templates. The method is characterized in...

20060183142 - Method of increasing discrimination for single base mismatch using hurdle dna and artificially mismatched probe: A method of increasing discrimination for a target DNA having a polymorphic site is provided. The method includes: synthesizing a first probe by artificially substituting a base of a probe which is fully complementary to a target DNA having a polymorphic site by a mismatched natural base and synthesizing a...

20060183144 - Methods and compositions for assessing nucleic acids: The present invention is directed to methods and compositions for evaluating nucleic acids, methods of preparing such compositions, and applications and business methods employing such compositions and methods. In particular, the present invention provides business methods for operating a gene expression measurement service....

20060183128 - Methods and compositions for differentiating tissues for cell types using epigenetic markers: The present invention provides, inter alia, a method for generating a genome-wide epigenomic map, comprising a correlation between methylation variable CpG positions (MVP) and genomic DNA sample types. MVP are those CpG positions that show a variable quantitative level of methylation between sample types. Particular genomic regions of interest (ROI)...

20060183130 - Methods for assessing p19-arf interactions with cmyc: The c-Myc oncogene is bound by p19Arf, which inhibits c-Myc's ability to transform cells while augmenting apoptosis. This provides the basis for screening assays that examine the ability of various candidate substances to promote p19Arf interactions, or to substitute therefor....

20060183152 - Methods of diagnosing, prognosticating and treating matrix metalloproteinase-1 related diseases via a matrix metalloproteinase-1 single nucleotide polymorphism: Methods and kits for diagnosing and prognosticating matrix metalloproteinase-1 related disease by detecting a single nucleotide polymorphism in the promoter of the gene are provided. Also provided are methods of identifying agents which inhibit binding of transcriptions factors to the Ets transcription factor binding site created by or resulting from...

20060183147 - Methods of identifying genes which modulate myelination: The present invention relates to methods of identifying and/or characterizing genes that are modulated during myelination or remyelination, as well as in demyelinating diseases, such as in multiple sclerosis. The invention further provides methods of treating diseases related to myelination in a mammal, such as multiple sclerosis, by administering agents...

20060183116 - Methods of use of the enzymes of mycothiol synthesis: The present invention utilizes three families of bacterial enzymes, which play a key role in mycothiol biosynthesis. The three families are bacterial cysteine:glucosaminyl inositol ligases (MshC) with catalytic ligase activity for ligation of glucosaminyl inositol and cysteine, bacterial acetyl-CoA:Cys-GlcN-Ins acetyltransferases (MshD) with catalytic activity for addition of an acetyl group...

20060183120 - Molecular sub-classification of kidney tumors and the discovery of new diagnostic markers: Genes that are differentially expressed in subtypes of renal cell carcinomas are disclosed as are their polypeptide products. This information is utilized to produce nucleic acid and antibody probes and sets of such probes that are specific for these genes and their products. Methods employing these probes, including hybridization and...

20060183114 - Novel eosinophil cationic proteins used in the treatement of asthematic and allergic disorders: The present invention relates to novel eosinophil cationic proteins characterised by the presence of a single nucleotide polymorphism. The presence of such polymorphism has been correlated to an advantageous phenotype being a reduced disposition to allergic and asthmatic disorders....

20060183133 - Novel fluorescent and colored proteins, and polynucleotides that encode these proteins: The subject invention provides new fluorescent and/or colored proteins, and polynucleotide sequences that encode these proteins. The subject invention further provides materials and methods useful for expressing these detectable proteins in biological systems....

20060183131 - Novel nucleotide and amino acid sequences, and assays and methods of use thereof for diagnosis of breast cancer: Novel markers for breast cancer that are both sensitive and accurate. These markers are overexpressed in breast cancer specifically, as opposed to normal breast tissue. The measurement of these markers, alone or in combination, in patient samples provides information that the diagnostician can correlate with a probable diagnosis of breast...

20060183118 - Nucleic acid recovery chip and nucleic acid recovery device: On a glass plate being transparent at specified wavelengths there are provided a laminated region being absorptive at the specified wavelengths; means for applying voltage to the region, the region exhibiting electrical conductivity; means for binding nucleic acids onto the region; a container for accommodating cells on the region; means...

20060183148 - Pna chip for determining genotypes of mycobacterial species using plastic substrate coated with epoxy group-containing polymer and method of determining genotypes of mycobacterial species using the pna chip: Provided are a PNA chip for determining the genotypes of mycobacterial species in which probe PNAs having DNA sequences of SEQ ID NOs: 5-28 are immobilized on a plastic substrate coated with an epoxy group-containing polymer, and a method of determining the genotypes of mycobacterial species using the PNA chip....

20060183123 - Polymerase-based protocols for the introduction of combinatorial deletions...: The present invention relates to improved methods of introducing combinatorial mutations in a region of DNA without the need for subcloning. The invention comprises polymerase-based, mutagenesis methods which may be adapted for use with commercially available mutagenesis kits to generate combinatorial mutations of a region of DNA in a quick,...

20060183139 - Polynucleotide associated with breast cancer comprising single nucleotide polymorphism, microarray and diagnostic kit comprising the same and method for diagnosing breast cancer using the same: Provided is a polynucleotide for diagnosis or treatment of breast cancer, including at least 10 contiguous nucleotides of a nucleotide sequence selected from the group consisting of nucleotide sequences of SEQ ID No: 1 to SEQ ID No: 5 and comprising a nucleotide at 101st position of the nucleotide sequence,...

20060183117 - Screening for alzheimer's disease: The present invention discloses methods of screening a subject for Alzheimer's disease comprising detecting the presence or absence of a marker or functional polymorphism associated with a gene linked to Alzheimer's disease....

20060183134 - Screening methods and sequences relating thereto: A screening method for identifying an individual having a pre-disposition towards having a cancer is disclosed, which screening method comprises the steps of: (a) obtaining a test sample comprising a nucleotide sequence comprised in the MYH gene of the individual or an amino acid sequence of a polypeptide expressed thereby;...

20060183132 - Selection probe amplification: Multiple unique selection probes are provided in a single medium. Each selection probe has a sequence that is complementary to a unique target sequence that may be present in a sample under consideration. For example, each selection probe may be complementary to a sequence that includes one of the SNPs...

20060183137 - Stress-regulated genes of plants, transgenic plants containing same, and methods of use: Clusters of plant genes that are regulated in response to one or more stress conditions are provided, as are isolated plant stress-regulated genes, including portions thereof comprising a coding sequence or a regulatory element, and to consensus sequences comprising a plant stress-regulated regulatory element. In addition, a recombinant polynucleotide, which...

20060183135 - Structure determining method of functional substance and method for producing functional substance: Novel technologies are provided to determine the structure of and produce a functional substance that has a high affinity to a target. Candidates for a substance having an affinity to a target (functional substance) is synthesized; a functional substance that has an affinity to the target is selected from among...

20060183124 - Target recognition element and biosensor including the same: The present invention provides a target recognizing element wherein a receptor is fixed to an inclusion complex containing a mediator. The target recognizing element includes a first host molecule having hydrophilic groups and an inclusion site, a second host molecule having hydrophilic groups and an inclusion site, a receptor which...

20060183150 - Tissue analysis and kits therefor: This invention relates to methods of analyzing a tissue sample from a subject. In particular, the invention combines morphological staining and/or immunohistochemistry (IHC) with fluorescence in situ hybridization (FISH) within the same section of a tissue sample. The analysis can be automated or manual. The invention also relates to kits...

20060183145 - Use of reversible extension terminator in nucleic acid sequencing: The present invention relates to the uses of reversible extension terminator in conjunction with optical confinements in nucleic acid sequencing. The apparatus and methods embodied in the present invention are particularly useful for high-throughput and low-cost nucleic acid sequencing....

20060183127 - Use of silver gene for the authenication of the racial origin of animal populations and of the derivative products thereof: The invention relates to the use of nucleotide sequences corresponding to the sliver gene, also known as the SI gene, and to the different allelic forms thereof, or corresponding to fragments of the gene and of the different allelic forms of same, in order to perform a method for the...

20060183155 - Compound libraries by glycotransferases and a method of preparing the same: The present invention aims to provide processes for rapidly and easily preparing compound libraries of known structures, and compound libraries prepared by said processes. A compound library of the present invention is prepared by a process for preparing a compound library in the same vessel, comprising: (1) mixing one or...

20060183154 - Human ring specific bnp antibodies: The present invention relates to antibodies that specifically bind to human BNP and immunoassays using said antibodies in the quantification of human BNP or a fragment of human BNP in a test sample....

20060183159 - Method for engineering a protein by in vitro coevolution: The present invention relates to compositions and methods for generating proteins with novel functions. The methods employ an in vitro coevolution approach that, in a stepwise manner, generates one or more intermediate functions. A pathway containing one or more analog molecules corresponding to the intermediate functions, and a target molecule,...

20060183153 - Method for screening substances capable of promoting oligomerization of receptor protein molecules: The present invention provides a method for screening substances that promote oligomerization of receptor protein molecules or peptide fragments thereof, which comprises determining whether the receptor protein molecules or peptide fragments thereof are oligomerized when contacted with a test substance; a test kit for screening substances that promote the oligomerization...

20060183157 - Methods and devices for characterizing macromolecular complexes using isotope labeling techniques: A method for characterizing interactions in macromolecular complexes, such as protein-protein or protein-ligand complexes, by selective isotopic labeling of the target molecule to reduce the 1H density in a selected spectral region; by irradiating the target; and by monitoring the polarization using filtered nuclear Overhauser spectroscopy (NOESY) and/or by performing...

20060183160 - Novel beta-secretase and modulation of beta-secretase activity: The invention concerns a novel β-secretase, a method of partially purifying this novel β-secretase, and its use in assays to screen for potential drug candidates against Alzheimer's disease and other neurological diseases. The novel β-secretase has an estimated molecular weight of about 32-39 kDa or 22-26 kDa in HEK293 cell...

20060183158 - Novel gene and use thereof: A protein and DNA encoding the same are useful as preventives/remedies for diseases such as hypoglycemia, etc. The protein of the present invention is also useful as a reagent for screening a compound that inhibits the binding of the protein of the present invention to IRAP (insulin responsive aminopeptidase) or...

20060183156 - Prpsc-selective peptides: The present invention features methods for identifying peptides that selectively bind to PrPSc or fragments thereof. The invention also features the use of such peptides in screening methods for the identification of candidate agents useful for the treatment of a prion disease....

20060183161 - Il-ili gene and polypeptide products: The present invention relates to the discovery of novel genes encoded by the interleukin-1 locus. The IL-1L1 gene is located in the interval between the IL-1Beta and the IL-1 receptor antagonist encoding genes and encodes a polypeptide which is homologous to both IL-1 agonist and IL-1 antagonist proteins. Therapeutics, diagnostics...

20060183163 - Methods and compositions for screening for modulators of ige synthesis, secretion and switch rearrangement: The invention relates to methods and compositions useful in screening for modulators of IgE synthesis, secretion and switch rearrangement....

20060183164 - Non-endogenous, constitutively activated known g protein-coupled receptors: The invention disclosed in this patent document relates to transmembrane receptors, more particularly to a human G protein-coupled receptor for which the endogenous ligand is known (“known GPCRs”), and most particularly to mutated (non-endogenous) versions of the known GPCRs for use, most preferably in screening assays for the direct identification...

20060183162 - Novel markers for alternatively activated macrophages: The present invention relates to novel markers for alternatively activated macrophages. More specifically, the present invention relates to the use of galactose-type C-type lectins as surface markers that allows rapid identification and sorting of the alternative macrophages. Such identifications can be useful in diseases where there is an imbalance between...

20060183167 - Novel assay to screen for anti-malarials: Malarial parasite Plasmodium falciparum is responsible for the most severe form of malaria in humans, causing about 2 million deaths every year. Lack of effective vaccines and emergence of drug-resistant strains necessitate the need of novel drug targets to treat the disease. The present invention describes a novel assay method...

20060183169 - Interleukin-8 homologous polypeptides and therapeutic uses thereof: The present invention is directed to novel polypeptides having structural homology to IL-8 and to nucleic acid molecules encoding those polypeptides. Also provided herein are vectors and host cells comprising those nucleic acid sequences, chimeric polypeptide molecules comprising the polypeptides of the present invention fused to heterologous polypeptide sequences, antibodies...

20060183168 - Method for optimizing therapeutic efficacy of nemorubicin: The present invention relates to products and methods for characterizing cancer patients in order to predict therapeutic benefit with a drug metabolized by CYP3A, especially nemorubicin....

20060183170 - Pretreatment kit for saliva and pretreatment method for saliva: A pretreatment kit and a pretreatment kit for saliva in identification and quantitative determination of mutans streptococci by immunochromatography utilizing an antigen-antibody reaction, which can remove aggregation caused by mucin and chain formation of mutans streptococci in saliva in a simple operation and can efficiently flow out a complex of...

20060183171 - High-throughput screening with multi-through hole testing plate: A high throughput screening method. At least one liquid sample is provided that includes an analyte that directly or indirectly produces a detectable characteristic in one or more first assays. A plurality of through holes is filled with portions of the liquid sample in a testing assembly that has a...

20060183166 - Arrays of supported biomembranes and uses thereof: A process for making a biomembrane array comprising providing a first substrate having a discrete zone and a second substrate. A first ink having a proteoliposome is then loaded onto the discrete zone to form a loaded zone. The loaded zone is then contacted to the second substrate such that...

20060183165 - Combination of chemical differentiators and their applications in mass sensing-based chemical sensor systems: A sensing unit comprises a chemical sensing element and a transducer operatively associated therewith, wherein the chemical sensing element comprises at least one receptor for receiving a molecule of an analyte. An array of chemical sensing units is also provided, together with a chemical sensor system and a method of...

20060183174 - Diagnosis: A method for screening, diagnosis or prognosis of heart failure in a mammalian subject, for determining the stage or severity of heart failure in a mammalian subject, for identifying a mammalian subject at risk of developing heart failure, or for monitoring the effect of therapy administered to a mammalian subject...

20060183175 - Diagnosis of preeclampsia: The present invention provides methods and compositions related to the detection and/or monitoring of the levels of angiogenic factors, specifically VEGF, PlGF and sFlt-1, in urine samples obtained from pregnant women and the effects of such levels on the risk of developing complications of pregnancy, including hypertensive disorders such as...

20060183172 - Diagnostic assay for thrombin-activatable fibrinolysis inhibitor (fafi): The invention relates to a diagnostic assay for selectively measuring levels of the 35kD form of thrombin-activatable fibrinolysis inhibitor (TAFla or TAFlai), or a derivative or variant thereof, but not the TAFI proenzyme (TAFI) or the N-terminal activation peptide of TAFI....

20060183173 - Protein achieving improved blocking efficiency: It is intended to provide a method of conveniently screening a novel protein or a novel partial sequence protein having a blocking ability based on amino acid sequence data; and a protein achieving an improved blocking efficiency that can be expressed on a large scale in Escherichia coli. A method...

20060183176 - Method and reagent for detecting cell death: A method for detecting cell death, which comprises determining cytochrome C in body fluid and detecting cell death based on a quantified value, as well as a method for determining cytochrome C in body fluid by a sandwich method and a reagent for measuring cytochrome C for determination cytochrome C...

20060183177 - Bioluminescent protease assay: A sensitive bioluminescent assay to detect proteases including caspases, trypsin and tryptase is provided....

20060183179 - Method for quantitatively determining ldl cholesterols: A method for quantitatively determining LDL cholesterol, including the steps of adding to serum a surfactant selected from among polyoxyethylenealkylene phenyl ethers and polyoxyethylenealkylene tribenzylphenyl ethers and a cholesterol-assaying enzyme reagent so as to preferentially react cholestrols in high density- and very low density-cholesterols among lipoproteins, and subsequently determining the...

20060183178 - Polypeptide formulations and methods for making, using and characterizing them: Embodiments of the invention include polypeptide formulations and methods for making, using and characterizing them. Embodiment of the invention include stabilized polypeptide formulations, for example stable glucose oxidase formulations that can be used with glucose sensors used in the management of diabetes. Another embodiment of the invention includes methods to...

20060183180 - Methods for treating pervasive development disorders: A method of utilizing the chymotrypsin level of an individual as a measure of the success of secretin, other neuropeptides, and peptides or digestive enzyme administration to such individuals, and in particular, as a prognosticative of potential secretin, other neuropeptides, peptides, and digestive enzyme administration for persons having ADHD, Autism...

20060183182 - Enzyme substrate: Coumarin derivative as a substrate for cytochrome P450 enzymes....

20060183181 - Methods and compositions for the detection and/or quantification of gram positive bacterial contaminants: The invention provides methods and compositions for the detection and/or quantification of a Gram positive bacterial contaminant in a sample. In particular, the invention provides hemocyte-based preparations, methods of making such hemocyte-based preparations, and methods of using such hemocyte-based preparations for the detection and/or quantification of the Gram positive bacterial...

20060183183 - Methods, compositions and kits for biological indicator of sterilization: A novel biological indicator system to detect the effectiveness of a sterilization treatment and methods for assessing the viability of and/or changes in bacterial spores exposed to a sterilization or disinfection method by multiangle light scattering thereby detecting a change in the spores as indicators of spore viability and the...

20060183185 - Method for the synthesis of astaxanthin: A method used for synthesizing intermediates for use in the synthesis of carotenoids and carotenoid analogs, and/or carotenoid derivatives. In some embodiments, the invention includes methods for synthesizing optically active intermediates useful for the synthesis of optically active carotenoids. Synthesis of optically active carotenoids, in one embodiment, may be accomplished...

20060183184 - Method of producing retinyl esters: A method of producing a retinyl ester compound comprising subjecting a composition comprising retinyl or a retinyl ester and a fat or oil of animal or vegetable origins to enzyme catalysed trans-esterification in solvent free conditions to produce a retinyl ester....

20060183187 - Gene and polypeptide sequences: The present invention provides a polypeptide comprising (i) a leader sequence, the leader sequence comprising a (a) secretion pre sequence, and (b) the following motif: -X1-X2-X3-X4-X5- where X1 is phenylalanine, tryptophan, or tyrosine, X2 is isoleucine, leucine, valine, alanine or methionine, X3 is leucine, valine, alanine or methionine, X4 is...

20060183186 - Gene expression profiles in stomach cancer: The present invention results from the examination of tissue from stomach tumors to identify genes that are differentially expressed between cancerous and normal tissue. The invention includes diagnostic, monitoring, drug design and therapeutic methods using these genes, as well as solid supports comprising oligonucleotide arrays that are complementary to or...

20060183188 - Genes from propionibacterium freudenreichii encoding enzymes involved in vitamin b12 biosynthesis: Four new genes, and the enzymes that they encode, are disclosed, which are present in Propionibacterium freudenreichii, and which are involved in at least five steps in the biosynthetic pathway of vitamin B12. The four enzymes are: A: cobyrinic acid a, c-diamide synthase; B: a bifunctional enzyme, which is a...

20060183194 - Heregulin variants: The present invention provides heregulin variants that are capable of binding an ErbB receptor. Included in the invention are variants of human heregulins, and, in particular, variants of human heregulin-β1 having enhanced affinity for the ErbB-3 and ErbB-4 receptors. These variants include at least one amino acid substitution and can...

20060183192 - Modulation of sodium channels in dorsal root ganglia: A novel tetrodotoxin resistant sodium channel is described, along with isolated nucleotides that encode this receptor. Methods for identifying agents that modulate the Na+ current through the receptor are provided, as well as related therapeutic and diagnostic methods....

20060183190 - Novel transporter protein: A novel protein which has an activity to transport hydantoin compounds is described, as well as a recombinant expressing this transporter protein. From Microbacterium liquefaciens strain AJ3912, a novel gene was discovered to encode a protein which is able to transport hydantoin compounds. A recombinant with an excellent ability to...

20060183196 - Polynucleotide encoding a novel cysteine protease of the calpain superfamily, can-12, and variants thereof: The present invention provides novel polynucleotides encoding CAN-12 polypeptides, fragments and homologues thereof. The present invention also provides polynucleotides encoding variants of CAN-12 polypeptides, CAN-12v1 and CAN-12v2. Also provided are vectors, host cells, antibodies, and recombinant and synthetic methods for producing said polypeptides. The invention further relates to diagnostic and...

20060183189 - Regulatable gene expression in mammalian cells and mammals: The present invention discloses mammalian cells useful for regulated gene expression in response to compounds being gaseous or liquid at the cultivation temperature of said mammalian cell, and non-human mammals comprising such mammalian cells. The invention further discloses methods for producing proteins in mammalian cells in a regulated way in...

20060183191 - Synthetic approach to designed chemical structures: This invention relates to the chemical design and production of peptides, peptide structure and three dimensional conformation was assessed using NMR, circular dichroisin and pulsed field gradient NMR....

20060183195 - Transgenic non-human animals for producing heterologous antibodies: The invention relates to transgenic non-human animals capable of producing high affinity human sequence antibodies. The invention is also directed to human sequence antibodies specific for human antigens, such as, human CD4. The invention also is directed to methods for producing human sequence antibodies....

20060183193 - Vectors and methods for high throughput co-expression: The present invention includes vectors and methods for high throughput co-expression....

20060183198 - Methods for expression and purification of recombinant human growth hormone: The present invention relates generally to the production, purification, and isolation of human growth hormone (hGH). More particularly, the invention relates to the production, purification, and isolation of substantially purified hGH from recombinant host cells or culture medium including, for example, yeast, insect, mammalian and bacterial host cells. The process...

20060183197 - Variant growth hormone molecules conjugated with macromolecules compounds: Conjugates exhibiting growth hormone (GH) activity and comprising at least one non-polypeptide moiety covalently attached to a GH polypeptide, the amino acid sequence of which differs from that of wildtype human GH in at least one introduced and at least one removed amino acid residue comprising an attachment group for...

20060183200 - Methods and means relating to protein variants: A method of providing a subject polypeptide variant with improved stability compared with a parent subject polypeptide, employing translation and selection using an RNA expression system, wherein two or more stability selection pressures are applied simultaneously during translation, two or more stability selection pressures are applied simultaneously during selecting, or...

20060183199 - Novel expression vectors containing accessory molecule ligand genes and their use for immunomodulation and treatment of malignancies and autoimmune disease: This invention relates to genes which encode accessory molecule ligands and their use for immunomodulation, vaccination and treatments of various human diseases, including malignancies and autoimmune diseases. This invention also describes the use of accessory molecule ligands which are made up of various domains and subdomain portions of molecules derived...

20060183201 - Autologous progenitor stem cells of gastrointestinal origin: This invention relates to the discovery of autologous stem cells of gastrointestinal origin in fecal matter. More particularly, the invention relates to the isolation and propagation of said stem cells in continuous culture. Furthermore, the invention describes a method of directing and converting these gastrointestinal progenitor stem cells into immunoglobulin...

20060183202 - Method for detoxification of mycotoxins: Disclosed is a method for detoxification of mycotoxins wherein mycotoxin is contacted with a glucosyltransferase in the presence of an activated glucose....

20060183203 - Polymer grafting by polysaccharide synthases: The present invention relates to methodology for polymer grafting by a polysaccharide synthase and, more particularly, polymer grafting using the hyaluronate synthase from Pasteurella multocida....

20060183204 - Process for manufacturing morpholino-nucleotides, and use thereof for the analysis of and labelling of nucleic acid sequences: in which n is an interger ranging from 1 to 12 and R3 is a group derived from a label, from a protein, from an enzyme, from a fatty acid or from a peptide, as chain terminators in a process of DNA or RNA sequencing by the Sanger method, or...

20060183205 - Method for controlling molecular weight and distribution of biopolymers: A method has been developed for the control of both molecular weight and molecular weight distribution of polyhydroxyalkanoates (PHAs) produced by bacterial strains. The control is exerted once the biopolymer has been accumulated intracellularly through a fermentation process by a chemical, enzymatic or irradiation treatment. More particularly, the control of...

20060183206 - Carrier for fixing material containing amino group: A carrier for fixing a material containing an amino group, which has a shape to be wound on a spindle member, and on the surface of which the material can be immobilized via covalent bonding. The carrier has a shape to be wound on a spindle member, and has a...

20060183208 - Activation of enzymes by lyophilization in the presence of solid inorganic support: Improved catalytic enzyme preparations are provided which effectively catalyze reactions carried out in organic solvents. The preparations comprise nanoscale solid support particles (e.g., fumed silica or alumina) having enzyme molecules electrostatically adhered to the surfaces of the particles. The preparations are prepared by mixing together solid support particles and enzyme...

20060183207 - Fen endonucleases: The present invention provides novel cleavage agents and polymerases for the cleavage and modification of nucleic acid. The cleavage agents and polymerases find use, for example, for the detection and characterization of nucleic acid sequences and variations in nucleic acid sequences. In some embodiments, the 5′ nuclease activity of a...

20060183209 - Enzymes for biopolymer production: In order to optimize the flux or flow of carbon intermediates from normal cellular metabolism into PHAs it is desirable to optimize the expression of the enzymes of the PHA biosynthetic pathway. Gene fusions are genetic constructs where two open reading frames have been fused into one and encode hybrid...

20060183210 - Human diacylglycerol acyltransferase 2 (dgat2) family members and uses therefor: The present invention relates to compositions and methods for the diagnosis and treatment of obesity and related metabolic disorders. The invention provides isolated nucleic acids molecules, designated DGAT2 family member nucleic acid molecules, which encode diacylglycerol acyltransferase family members. The invention also provides recombinant expression vectors containing DGAT2 family member...

20060183211 - Novel aromatic prenyltransferases, nucleic acids encoding same and uses therefor: In accordance with the present invention, a novel aromatic prenyltransferase, Orf2 from Streptomyces sp. strain CL190, involved in naphterpin biosynthesis has been identified and the structure thereof elucidated. This prenyltransferase catalyzes the formation of a C—C bond between a prenyl group and a compound containing an aromatic nucleus, and also...

20060183213 - Microbially-expressed thermotolerant phytase for animal feed: The invention provides methods for making and using thermotolerant phytases, e.g., a method of using a thermotolerant phytase in feed and food processing and feed or food products comprising a thermotolerant phytase....

20060183212 - Thermostable luciferases and methods of production: Luciferase enzymes with greatly increased thermostability, e.g., at least half lives of 2 hours at 50° C., cDNAs encoding the novel luciferases, and hosts transformed to express the luciferases, are disclosed. Methods of producing the luciferases include recursive mutagenesis. The luciferases are used in conventional methods, some employing kits....

20060183214 - Modulation of plasma membrane human leukocyte elastase: A method for modulation of plasma membrane associated Human Leukocyte Elastase (HLE) to inflammatory states by interaction of HLE with an antagonist to inhibit HLE and thereby interruption in plasma associated events (e.g. HIV disease progression, bacterial infections and autoimmune diseases), which are responsive/sensitive to such inflammation. The antagonist suitable...

20060183215 - Substance injecting apparatus: A particle fixing unit fixes a particle. A needle is arranged in such a manner that an angle between a surface on a side of the particle fixing unit on which the particle is fixed and a longitudinal center axis of the needle becomes equal to or more than 45...

20060183216 - Containers for liquid storage and delivery with application to microfluidic devices: A container for a liquid reagent, wherein the container has an outer wall and an internal piercing member, such that, upon application of pressure to the outer wall of the container, the internal piercing member punctures the container from the inside, thereby liberating the liquid contained therein. Such a container...

20060183217 - Cartridge for automatic measurement and measuring device using the same: An object of the present invention is to provide a cartridge for automatic measurement used in an automatic measuring device, capable of automatically performing measurement including heat treatment of a sample, and a measuring device using the cartridge. The present invention relates to a cartridge for use in measuring a...

20060183218 - Material for forming fine pattern and method for forming fine pattern using the same: In the method wherein a resist pattern is miniaturized effectively by applying a fine pattern forming material, the fine pattern forming material used for providing with a cured coated layer pattern, wherein development defects are reduced by water development is offered, wherein the fine pattern forming material comprises a water-soluble...

20060183219 - Patterning substrate and cell culture substrate: The present invention intends primarily to provide such as a cell culture patterning substrate that is used to adhere cells in a highly precise pattern on a base material to culture and a cell culture substrate on which cells are adhered in a highly precise pattern. To attain the object,...

20060183220 - Automated cell sample enrichment preparation method: A method for automated cell enrichment preparation where biological cells are injected into a first subchamber and flowed through the first subchamber, through a large pore filter and into a second subchamber so that the large pore filter retains particles large particles in the first subchamber, while the biological cells...

20060183221 - Method for neural differentiation of embryonic stem cells using protease passaging techniques: The present invention provides methods for human pluripotent cell culturing and for neural cell production. More particularly, the present invention provides culturing methods employing dissociating cell cultures to an essentially single cell culture, such as by employing antibody selection and bulk passaging treatments utilizing the subsequent application of Collagenase and...

20060183222 - Method for culturing neurons, neuron culture substrate, neurons, neuron system, and method for manufacturing neuron system: There is provided a method for controlling morphological growth of neurons by specifically controlling the surface configuration of a substrate. There is also provided a neuron culture substrate necessary for application of the method and neurons controlled in morphological growth. The method for culturing neurons include, providing a culture medium...

20060183223 - Continuous flow chamber device for separation, concentration, and/or purification of cells: The present invention relates to methods and apparatuses for cell separation. In particular, the invention relates to separation of a particular cell type from a mixture of different cell types based on the differential rolling property of the particular cell type on a substrate coated with molecules that exhibits adhesive...

20060183224 - Animal-free cell culture method: The present invention relates to a process for animal, preferably human, diploid anchorage-dependent cell culture, in the absence of exogenous components of primary animal origin, and to a cell culture medium substantially free of exogenous components of primary animal origin suitable for carrying out said process. In particular the invention...

20060183225 - Method of enhancing homologous recombination of somatic cells and method of constructing specific antibody: The present invention provides a novel method for obtaining diverse antibodies as a result of markedly enhancing the somatic homologous recombination at an antibody locus in immunocytes. By putting immunocytes in which DNA homologous recombination is occurring at an antibody locus (for example, DT40 cells and the like) into contact...

20060183228 - viral vectors with surface or envelope components: Provided are novel vectors and viral vectors capable of expressing exogenous gene or exogenous nucleic acid sequences in a target cell of interest, such as T cells, bone marrow cells, epithelial cells, liver cells and the like. The nucleic acid components of the vectors may include one or more native...

20060183227 - Method for gene transfer into the organelles of cells: direct gene transfer to mitochondria: Methods of gene transfer into cellular organelles are disclosed which provide the necessary conditions for genetic materials such as virus or recombinant DNA to be delivered directly into mitochondria from extracellular space in vitro or in vivo. Once inside the mitochondria, the genetic materials can then be translated according to...

20060183226 - Methods for retrotransposing long interspersed elements (lines): The present invention provides methods for retrotransposing LINEs. The present invention relates to methods for transcribing RNAs comprising LINE 3′UTR fragments in cells, and retrotransposing these RNAs by using viral vectors to provide their LINE ORF proteins in trans. This invention also relates to methods for altering LINE retrotransposition target...

20060183231 - Multiple-compartment eukaryotic expression systems: Method and constructs for expressing heterologous sequences of interest in eukaryotic cells using multiple-compartment expression systems. These systems, which may be comprised of a single construct or multiple constructs, utilize at least two different promoters which are each active within a different subcellular compartment of the same eukaryotic cell. The...

20060183232 - Packaging cells for recombinant adenovirus: In the absence of substantial sequence overlap between a recombinant adenoviral vector and the genome of a packaging cell, helper-dependent E1-containing particles (HDEP) can be formed at low frequency. The invention provides means and methods reducing or preventing the generation of HDEP. To this purpose, novel packaging cells and methods...

20060183229 - Regulatory constructs comprising intron 3 of prostate specific membrane antigen gene: The invention provides a recombinant vector comprising an ovine adenovirus genome and a sequence encoding a heterologous polypeptide, wherein the sequence encoding the heterologous polypeptide is inserted between E4 and E3 transcription units of the ovine adenovirus genome....

20060183230 - Vectors, cell lines and their use in obtaining extended episomal maintenance replication of hybrid plasmids and expression of gene products: This disclosure shows that the BPV-1 E2 protein-dependent minichromosome maintenance element (MME) comprised of E2 multimeric binding sites can provide the stable maintenance replication function to the mouse polyomavirus (PyV) core origin plasmids in the presence of BPV-1 E2 protein and PyV large T-antigen (LT). MME dependent plasmids are lost...

20060183233 - Transformant having an increased frequency of homologous recombination: This invention relates to a transformant having an increased frequency of homologous recombination (gene targeting), which is a mitosporic filamentous fungus belonging to Trichocomaceae, due to suppression of a ku gene by ku gene disruption or antisense RNA method, to a method for the production of a gene-disruption stain, gene-deletion...

20060177810 - Method of inhibiting freeze concentrating of substance in wet material, method of inhibiting deactivation of physiologically active substance, and process for producing lyophilizate or frozen matter having component homogeneously diffused therein: An antifreeze protein is added to a hydrous material when the hydrous material contains water molecules and a substance other than water molecules, and freeze concentration of the substance should be inhibited; when the hydrous material contains a bioactive substance and inactivation of the bioactive substance should be inhibited; or...

20060177811 - Composition for preserving platelets and method of using the same: The present invention relates to compositions and methods for storing platelets to preserve the function and freshness of the platelets. More particularly, the present invention relates to the use of a preservative composition having an antiplatelet agent, an anticoagulant, and an oxygen carrier, for maintaining the freshness of platelets. Additionally,...

20060177816 - Cellular rhogtpase activation assay: The invention relates to a cellular RhoGTPase activation assay based on the determination of changes in the actin cytoskeleton (actin cytoskeletal rearrangement assay); to the recombinant cell lines used to carry out this assay; to the constructs required to produce these cell lines; to the use of these recombinant cell...

20060177815 - Dielectrophoretic particle sorter: Provided are methods, devices and systems that utilize dielectrophoretic forces to separate a target species from a plurality of species in a sample....

20060177812 - Method and device for manipulating samples: The invention relates to a method and a device for manipulating samples, in particular tissue samples, comprising at least one needle (2) for punching holes (34) in sample carriers (4) and at least one further needle (3) for punching samples out of preparations (5), wherein a device for detecting the...

20060177813 - Novel high-throughput screening method of drug for bioactive protein: The present invention is intended to provide a safe and quick means for screening a drug to a bioactive protein, in particular, an inhibitor, using a cell-free protein synthesis system with the use of a wheat embryo extract solution. The present inventors have strenuously studied to solve the matters above...

20060177814 - Systemic lupus erythematosus: This document relates to methods and materials involved in diagnosing SLE. For example, this document relates to methods and materials involved in diagnosing SLE, diagnosing severe SLE, and assessing a mammal's susceptibility to develop severe SLE. For example, this document provides nucleic acid arrays that can be used to diagnose...

20060177819 - Alphavirus replicon vector systems: The present invention provides compositions useful in and methods for producing populations of infectious, replication-defective alphavirus replicon particles that contain no replication competent alphavirus particles, as determined by passage on cells in culture. The compositions include helper and replicon nucleic acid molecules that can further reduce the predicted frequency for...

20060177817 - Codon-optimized papilloma virus sequences: Synthetic DNA molecules encoding the HPV31 L1 protein are provided. Specifically, the present invention provides polynucleotides encoding HPV31 L1 protein, wherein said polynucleotides are free from internal transcription termination signals that are recognized by yeast. Also provided are synthetic polynucleotides encoding HPV31 L1 wherein the polynucleotides have been codon-optimized for...

20060177818 - Method of detection of classical swine fever: The invention relates to a multiplex real-time RT-PCR assay with a heterologous internal control system (i.e., EGFP-RNA) for the simple and fast diagnosis of classical swine fever virus (CSFV). Primers and FAM-labeled TaqMan probes, specific for CSFV were selected by analyzing the consensus sequence of the 5′-non translated region of...

20060177820 - Srsv detection kit: This invention relates to an SRSV detection kit comprising all antibodies against SRSV-related virus constituting peptides selected from the following peptide groups (a) to (k), respectively: (a) a peptide having an amino acid sequence represented by SEQ ID NO: 1, and the like, (b) a peptide having an amino acid...

20060177828 - Alternatively spliced isoform of mitotic centromere-associated kinesin (mcak): The present invention features a nucleic acid and a polypeptide encoding a novel splice variant isoform of mitotic centromere-associated kinesin (MCAK). The polynucleotide sequence of MCAKsv1 is provided by SEQ ID NO 2. The amino acid sequence for MCAKsv1 is provided by SEQ ID NO 3. The present invention also...

20060177853 - Antidepressant drug target: The galaninergic system of the dorsal raphe nucleus is employed as a target for screening drug candidates for antidepressant activity....

20060177821 - Association of the h2 haplotype of the p2y12 receptor with an increased risk for thrombosis and peripheral arterial disease: The invention relates to an in vitro method for determining the risk of developing thrombosis in a subject, and to an in vitro method for determining sensitivity of a subject toward a thienopyridine therapy. Both methods involve identifying a particular haplotype of the P2Y12 receptor gene....

20060177863 - Biallelic markers for use in constructing a high density disequilibrium map of the human genome: The present invention relates to genomic maps comprising biallelic markers, new biallelic markers, and methods of using biallelic markers. Primers hybridizing to regions flanking these biallelic markers are also provided. This invention provides polynucleotides and methods suitable for genotyping a nucleic acid containing sample for one or more biallelic markers...

20060177843 - Bioarray chip reaction apparatus and its manufacture: This invention provides oligonucleotide based arrays and methods for speciating and phenotyping organisms, for example, using oligonucleotide sequences based on the Mycobacterium tuberculosis rpoB gene. The groups or species to which an organism belongs may be determined by comparing hybridization patterns of target nucleic acid from the organism to hybridization...

20060177831 - C-met kinase binding proteins: Polypeptides comprising monomer domains that bind to c-MET, or portions thereof, are provided....

20060177835 - Cleavage of nucleic acids: The present invention relates to means for the detection and characterization of nucleic acid sequences, as well as variations in nucleic acid sequences. The present invention also relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a...

20060177844 - Closed-system multi-stage nucleic acid amplification reactions: The invention is directed to systems, methods, and apparatus for carrying out multi-stage amplification reactions, especially under fluidly closed conditions. In one aspect, methods of the invention are carried out in a fluidly closed reaction system that permits the isolation of a portion of a first (or prior) reaction mixture...

20060177859 - Compositions and methods for detecting group b streptococci: Compositions, methods and kits for detecting Group B streptococci. Particularly described are oligonucleotides that are useful as amplification primers and hybridization probes for detecting very low levels of Group B streptococci nucleic acids....

20060177865 - Computational method for designing enzymes for incorporation of amino acid analogs into proteins: The instant invention provides methods, reagents, and computational tools for designing non-natural substrate analogs for enzymes, especially for designing unnatural amino acid analogs for aminoacyl tRNA Synthetases (AARSs), such as the Phe tRNA Synthetase. The instant invention also provides methods to incorporate unnatural amino acid analogs, especially those with interesting...

20060177857 - Dark quenchers, probes and other conjugates incorporating the same, and their use: e

20060177832 - Genetic analysis by sequence-specific sorting: The invention provides methods for sorting polynucleotides from a population based on predetermined sequence characteristics. In one aspect, the method of the invention is carried out by extending a primer annealed polynucleotides having predetermined sequence characteristics to incorporate a predetermined terminator having a capture moiety, capturing polynucleotides having extended primers...

20060177860 - Genetic markers in the hla-dqbi gene associated with an adverse hematological response to drugs: Genetic markers in the HLA-DQB1 gene associated with adverse hematological response to drug therapy are disclosed. Compositions and methods for detecting and using these HLA-DQB1 markers in a variety of clinical applications are disclosed. Such applications include methods for testing an individual for susceptibility for an adverse hematological response, methods...

20060177851 - Genetic markers of schizophrenia: The invention includes method of determining if a subject is at risk for developing schizophrenia (SZ), schizotypal personality disorder (SPD), or schizoaffective disorder (SD)....

20060177825 - Global analysis of transposable elements as molecular markers of the developmental potential of stem cells: This invention relates to the determination of expression patterns, DNA methylation patterns and chromatin properties of families of transposable elements in order to determine, classify and characterize the potential of stem cells to differentiate into germ layers including various types of somatic cell lineages....

20060177822 - Glycosyltransferase, nucleic acid encoding the glycosyltransferase and method of testing canceration using the nucleic acid: A tumor marker nucleic acid of the present invention is concerned with a nucleic acid hybridizing under stringent conditions to a nucleotide sequence described in SEQ ID NO: 1 or a complementary nucleotide sequence thereof. A method of testing canceration of the present invention is a method comprising diagnosing a...

20060177854 - Hereditary hemochromatosis gene: The invention relates generally to the gene, and mutations thereto, that are responsible for the disease hereditary hemochromatosis (HH). More particularly, the invention relates to the identification, isolation, and cloning of the DNA sequence corresponding to the normal and mutant HH genes, as well as the characterization of their transcripts...

20060177847 - Markers for metabolic syndrome obesity and insulin resistance: Correlations between polymorphisms and metabolic syndrome, obesity, treatment-emergent weight gain and insulin resistance are provided. Methods of diagnosing and treating metabolic syndrome, obesity, treatment-emergent weight gain and insulin resistance are provided. Systems and kits for disgnosis and treatment of metabolic syndrome, treatment-emergent weight gain, obesity and insulin resistance are provided....

20060177846 - Method and apparatus for design and display of primers: A technology that allows a consensus sequence targeted for primer design to be easily displayed is provided. In sequence data of analysis targets, a consensus nucleotide sequence is generated by performing multiple sequence alignment based on nucleotide sequence, and a consensus amino acid sequence is generated by performing multiple sequence...

20060177827 - Method computer program with program code elements and computer program product for analysing s regulatory genetic network of a cell: A regulator genetic network of a cell is analyzed using a causal network after predefining a gene expression rate for a selected gene of the regulatory genetic network. The causal network is used to generate a resultant gene expression pattern relating to the genetic network for the predefined gene expression...

20060177856 - Method for determining a concentration of target nucleic acid molecules, nucleic acid probes for the method and method for analyzing data obtained by the method: A method is provided for determining a concentration of a target nucleic acid by using a nucleic acid probe labeled with a fluorescent dye. The method comprises: providing, as the probe, a nucleic acid probe capable of reducing fluorescence emission from the fluorescent dye when hybridized with the target nucleic...

20060177839 - Method for modulating the evolution of a polypeptide encoded by a nucleic acid sequence: A method for modulating the ability of a gene to mutate by analyzing codon usage within the gene and selecting a synonymous nucleotide sequence with a higher, lower or different capacity to mutate. The method permits widening and optimization of the evolutionary landscape of a protein. A computer-implemented method for...

20060177830 - Method of detection of predisposition to emphysema in chronic obstructive pulmonary disease: The present invention relates to a method of detection of predisposition to emphysema in chronic obstructive pulmonary disease (COPD). It particularly relates with the regulation of the key molecular and biochemical components of the pathway leading to the manifestation of emphysema in COPD....

20060177826 - Method of producing recombinant dna molecules: The present invention is directed to an improved method for producing by recombinant methods proteins that occur in nature in two or more subunits; more generally, it is applicable to the amplification and subsequent expression of any chimeric DNA molecule that results form the ligation of two or more non-contiguous...

20060177868 - Method of spotting probe on solid support, probe array and method of manufacturing thereof, and method of detecting target substance and method of identifying structure of target substance using probe array: Provided is a method of spotting a probe densely and efficiently on a surface of a solid support. A liquid containing a probe is attached to a solid support as droplets to form spots containing the probe on the solid support by an ink jet method....

20060177833 - Methods and compositions for tagging and identifying polynucleotides: The invention provides methods and compositions for attaching oligonucleotide tags to polynucleotides for the purpose of carrying out analytical assays in parallel and for decoding the oligonucleotide tags of polynucleotides selected in such assays. Words, or subunits, of oligonucleotide tags index submixtures in successively more complex sets of submixtures (referred...

20060177864 - Methods for identifying drug combinations for the treatment of proliferative diseases: The invention features methods for identifying new combination therapies for the treatment of cancer and other proliferative diseases....

20060177840 - Methods for separation of polymeric compounds: Recently two techniques using free solution electrophoresis to separate charged-uncharged polymer conjugates have proven successful: End Labeled Free Solution Electrophoresis (ELFSE) for DNA sequencing, and Free Solution Conjugate Electrophoresis (FSCE) for molar mass profiling of uncharged polymers. Previous attempts have been made to analyze experimental data generated by these new...

20060177838 - Methods for treatment with bucindolol based on genetic targeting: The present invention concerns the use of methods for evaluating bucindolol treatment for a patient, particularly one with heart failure. It concerns methods for determining whether to administer or prescribe bucindolol to a patient based on whether the patient is homozygous for the Arg 389 polymorphism in the β1-adrenergic receptor...

20060177848 - Methods of identifying individuals at reduced risk of sepsis: The present invention provides a method of identifying a subject having a reduced risk of developing sepsis, comprising detecting at least one APOE3 allele in nucleic acid from the subject....

20060177836 - Methods of isolating nucleic acids using multifunctional group-coated solid phase carriers: The present invention is directed to a method of isolating a target species (e.g., target nucleic acid species) from a mixture. In the methods of the present invention, the mixture is combined with solid phase carriers having a surface comprising multiple functional groups one of which reversibly and selectively binds...

20060177829 - Methods to identify compounds useful for tumor sensitization to dna damage: Cdc25A is herein identified as a substrate for β-TrCP1- or β-TrCP2-mediated ubiquitination and subsequent degradation via the ubiquitin-proteasome pathway. In particular, it has been found that interfering with β-TrCP expression or function, or increasing β-TrCP degradation, leads to accumulation of Cdc25A in a cell. Since degradation of Cdc25A is a...

20060177823 - Molecules: The present invention describes the chromosomal location of obesity susceptibility genes and provides methods for detecting the presence of such genes. The present invention enables the development of novel therapies to treat obesity....

20060177855 - Nanoparticles for manipulation of biopolymers and methods of thereof: Matrices for manipulation of biopolymers, including the separation, purification, bilization and archival storage of biopolymers is disclosed....

20060177861 - Novel compositions and methods in cancer associated with altered expression of mcm3ap: The present invention relates to novel sequences for use in diagnosis and treatment of carcinomas, especially breast cancers. In addition, the present invention describes the use of novel compositions for use in screening methods. The invention provides methods and compositioins associated with altered expression of MCM3AP....

20060177849 - Nucleic acid primer set, nucleic acid probe set and method for detecting respiratory disease virus using the primer set and probe set: Provided are a nucleic acid primer set capable of simultaneously amplifying the target sequences of five or more respiratory disease viruses and including oligonucleotides each of which comprises a fragment of at least 10 contiguous nucleotides present in sequences as set forth SEQ ID NOS: 1-26, a nucleic acid probe...

20060177858 - Optimized probe selection method: The present invention provides methods for optimizing oligonucleotide hybridization probes for use in basic and clinical research. Specifically, the invention involves hybridizing serially diluted genomic sample to the oligonucleotide probes on the array, such that a signal intensity is produced for each of the probes; computationally identifying optimized probes which...

20060177850 - Particle-based multiplex assay system with three or more assay reporters: A system and method for developing and utilizing particle-based n-multiplexed assays that include three or more reporters utilizes n particle sets that are associated with particle identification images or labels (IDs) that differ between sets. The encoded particles for a given set are coated with a specific binding member, or...

20060177841 - Primers, probes and methods for nucleic acid amplification: Homogenous detection during or following PCR amplification, preferably LATE-PCR, utilizing fluorescent DNA dye and indirectly excitable labeled primers and probes, improves reproducibility and quantification. Low-temperature homogeneous detection during or following non-symmetric PCR amplification, preferably LATE-PCR, utilizing fluorescent DNA dye and indirectly excitable labeled mismatch-tolerant probes permits analysis of complex targets....

20060177842 - Reagents and methods for improving reproducibility and reducing mispriming in pcr amplification: An additive for preventing mispriming in polymerase chain reaction (PCR) amplifications and assays comprising a hairpin oligonucleotide having a stem duplex greater than six nucleotides in length and a stabilized stem terminus. The additive improves PCR amplifications, including LATE-PCR amplifications when added to initial amplification reaction mixtures. It can be...

20060177867 - Repair of nucleic acids for improved amplification: Methods and compositions are provided for repairing a polynucleotide so that it can be copied with improved fidelity and/or yield in, for example, an amplification reaction. This involves the use of a reaction mixture that includes a ligase and a cofactor selected from NAD+ or ATP and incubating the polynucleotide...

20060177862 - Ribosome display: The use in a ribosome display system for selection of a specific binding pair member (e.g. antibody molecule) able to bind a complementary specific binding pair member (e.g. antigen) of encapsidating specific binding member/ribosome complexes in a viral coat, optionally in combination with incorporation of an Midvariant RNA template and...

20060177824 - Salmonella detection identification: A nucleic acid specific for use in detecting and differentiating Salmonella from other bacteria comprises at least 10 contiguous nucleotides and is capable of selectively hybridizing to at least a portion of the prg gene of Salmonella....

20060177852 - Solid-fluid composition: A nanostructure comprising a core material of a nanometric size surrounded by an envelope of ordered fluid molecules is disclosed. The core material and the envelope of ordered fluid molecules are in a steady physical state. Also disclosed, a liquid composition comprising liquid and the nanostructure....

20060177837 - Systems and methods for identifying diagnostic indicators: Systems and methods are provided for predicting patient response to a therapy regimen for a liver disease or a disease that is treatable with an immunomodulatory disease therapy using gene expression classifiers. Systems and methods for screening for modulators of target gene expression are also provided. Systems and methods for...

20060177866 - Transmembrane serine protease overexpressed in ovarian carcinoma and uses thereof: The present invention provides a TADG-12 protein and a DNA fragment encoding such protein. Also provided is a vector/host cell capable of expressing the DNA. The present invention further provides various methods of early detection of associated ovarian and other malignancies, and of interactive therapies for cancer treatment by utilizing...

20060177845 - Ubp1 protease mutant, and its coding sequence, their application and methods of production: A UBP1 protease mutant and the sequence coding it, their application and products and the methods used to produce them may be used in the production of recombinant proteins, particularly on an industrial scale....

20060177869 - Analysis of compounds: The analysis of compounds utilising the physical response of cellular networks is described. Typically, the electrical characteristics of the cellular network are monitored although other characteristics such as fluorescence may be monitored. In any event, the analysis utilises signal processing techniques to derive a set of features which may be...

20060177872 - Diagnostic test for vitamin b12: An isolated nucleotide sequence or fragment thereof encoding the porcine intrinsic factor, wherein the porcine intrinsic factor comprises an amino acid sequence having at least 85% amino acid sequence identity to an amino acid sequence selected from the group consisting of SEQ ID NO: 3, SEQ ID NO: 6, and...

20060177870 - Immunoassays: Methods for discriminating between related proteins and other analytes in a sample and for correlating protein expression with disease states....

20060177873 - Method of adjusting the working range of a multi-analyte assay: The invention features a method of adjusting the concentration of at least one but not all of a plurality of analytes in a fluid sample to match a known working range of detection of an analyte assay system, where each of the plurality of analytes may or may not be...

20060177871 - Murine il-18 crystal structure: A novel murine IL-18 native crystalline structure is identified....

20060177874 - Sensing switch and detecting method using the same: Provided are a sensing switch and a sensing method using the same. The sensing switch includes: a substrate; a supporter on the substrate; a sensing plate that is connected to a side of the supporter and is in parallel with the substrate by a predetermined distance; a receptor binding region...

20060177878 - Animal cell colony picking apparatus and method: An apparatus and method for automated picking of animal cell colonies. A picking head comprising a plurality of hollow pins is provided. The apparatus has an integrated imager for capturing an image of adherent or non-adherent animal cell colonies held in liquid or semi-solid medium. Image processing identifies the locations...

20060177877 - Methods for identifying combinations of entities as therapeutics: The invention features a method of screening two-entity or higher order combinations for biological activity using combinational arrays. The method includes the steps of: (a) providing the entities, (b) creating an array of combinations of entities, (c) providing a test element that includes one or more distinct biological moieties, (d)...

20060177876 - Monoclonal antibody against asialo alpha 1-acid glycoprotein, immunochromatographic strip comprising the monoclonal antibody, and method for diagnosing liver diseases using the immunochromatographic strip: The present invention relates to a method for diagnosing a liver disease rapidly in an early stage. More particularly, the present invention relates to a monoclonal antibody against asialo α1-acid glycoprotein; a method for diagnosing a liver disease which evaluates asialo α1-acid glycoprotein in a test sample by using said...

20060177875 - Tryptase polypeptide and uses thereof: The present invention provides a purified expressed δ tryptase polypeptide or fragment or analogue thereof, in particular, the human δ tryptase polypeptide or fragment or analogue thereof. The present invention also provides variants of such polypeptides. The invention further relates to methods of diagnosing disease states based on expression of...

20060177880 - Use of prn3/ileu as a marker for colorectal cancer: The present invention relates to the diagnosis of colorectal cancer. It discloses the use of protein PRN3/ILEU and/or unbound PRN3 in the diagnosis of colorectal cancer. It relates to a method for diagnosis of colorectal cancer from a liquid sample, derived from an individual by measuring PRN3/ILEU and/or unbound PRN3...

20060177881 - Antibodies against type a botulinum neurotoxin: Antibodies for binding epitopes of BoNT/A and hybridomas which produce such antibodies are described. The antibodies of the present invention can be used in a method for detecting BoNT/A in a sample and/or in a method for purifying BoNT/A from an impure solution. In addition, the antibodies can be used...

20060177879 - Magnetic nanoparticles and method of fabrication: A method for making a composition of magnetic nanoparticles. The method includes the step of forming the magnetic nanoparticles, each within a protein template, wherein a liquid composition of said protein templates or subunits thereof is subjected to a microporous membrane filtration step prior to formation of said magnetic nanoparticles....

20060177883 - 5-fluoro-uracil immunoassay: Novel conjugates of 5-fluoro-uracil and novel 5-fluoro-uracil immunogens and monoclonal antibodies generated by these immunogens which are useful in immunoassays for the quantification and monitoring of 5-fluoro-uracil in biological fluids....

20060177884 - 5-fluoro-uracil immunoassay: Novel conjugates of 5-fluoro-uracil and novel 5-fluoro-uracil immunogens and monoclonal antibodies generated by these immunogens which are useful in immunoassays for the quantification and monitoring of 5-fluoro-uracil in biological fluids....

20060177882 - Immunoassays with enhanced selectivity: The present invention provides a method for the detection of an analyte in a specimen comprising two steps. First, the specimen is contacted with a substrate having receptors bound thereon, having higher affinity for cross-reactive substances than for the analyte, in order to increase the ratio of the analyte to...

20060177885 - Delta 4,5 glycuronidase and methods of analyzing therewith: The invention relates to Δ4,5 glycuronidase, related compositions, and methods of use thereof....

20060177886 - Method for diagnosis of rheumatoid arthritis: The present invention aims at providing a method for the diagnosis of RA. The present invention relates to a method for the diagnosis of rheumatoid arthritis, comprising measuring the dipeptidyl peptidase activity in a sample collected from a mammal....

20060177887 - Method of analyzing a protein: Provided a method of acquiring information on internal sequence which is applicable to a trace amount of protein and has a high reliability. A protein to be analyzed is limitedly cleaved at a specified position of amino acid to prepare a mixture of a plurality of peptide fragments; one or...

20060177888 - Method for evaluating antioxidation capability of organism sample: Herein provided is a method for evaluating the antioxidant potential of a biological sample comprising the steps of: (a) collecting a sample containing at least oxidizable substrates from a living body; (b) initiating an oxidation reaction of said oxidizable substrates; (c) continuing said oxidation reaction; and (d) quantifying oxidation products...

20060177889 - Rapid responding gas sensing element: A gas analysis sensing element and a method of making the sensing element is disclosed. In one embodiment, the sensing element includes cytochrome-c embedded in a sol-gel matrix. The sol-gel matrix may take the form of a thin film or a monolith. The applicants have discovered a number of parameters...

20060177890 - Rapid-response gas sensing element: A gas analysis sensing element and a method of making the sensing element are disclosed. In one embodiment, the sensing element includes cytochrome c embedded in a sol-gel matrix. The sol-gel matrix is either a thin film or a monolith. Parameters for creating such a sensing element, including protein concentration,...

20060177891 - Fiber optic bio-sensor: A sensor for sensing and monitoring a property associated with transformation of a biochemical analyte by a micro-organism, the sensor comprising a glass permeable coating applied to an unclad portion (14) of a fibre optic member (13). The coating has a transformable precursor impregnated into it, the precursor being specifically...

20060177892 - Intracellular formation of peptide conjugates: The invention includes methods and compositions for forming peptide conjugates intracellularly having a covalent linkage between a modifying group and a glycosylated or non-glycosylated peptide. The modifying group is conjugated to the peptide via a glycosyl linking group interposed between and covalently linked to both the peptide and the modifying...

20060177893 - Method for producing dipeptides: The present invention provides a method for producing a dipeptide from starting materials that are available at low costs through a route industrially advantageous and simple. Dipeptides are produced from amino acid esters and amino acids by using a culture of a microbe having an ability to produce a dipeptide...

20060177899 - 14273 receptor, a novel g-protein coupled receptor: The present invention relates to a newly identified receptor belonging to the superfamily of G-protein-coupled receptors. The invention also relates to polynucleotides encoding the receptor. The invention further relates to methods using the receptor polypeptides and polynucleotides as a target for diagnosis and treatment in receptor-mediated disorders, specifically, cardiovascular diseases,...

20060177896 - Anti-cd3 antibodies and methods of use thereof: The present invention is related to antibodies directed to the antigen CD3 and uses of such antibodies. In particular, the present invention provides fully human monoclonal antibodies directed to CD3. Nucleotide sequences encoding, and amino acid sequences comprising, heavy and light chain immunoglobulin molecules, particularly sequences corresponding to contiguous heavy...

20060177900 - Compositions of orthogonal lysyl-trna and aminoacyl-trna synthetase pairs and uses thereof: Compositions and methods of producing components of protein biosynthetic machinery that include orthogonal lysyl-tRNAs, orthogonal lysyl-aminoacyl-tRNA synthetases, and orthogonal pairs of lysyl-tRNAs/synthetases, which incorporate homoglutamines into proteins are provided in response to a four base codon. Methods for identifying these orthogonal pairs are also provided along with methods of producing...

20060177902 - Cytokine receptor chain: Polynucleotides encoding the IL-13 receptor and fragments thereof are disclosed. IL-13 receptor proteins, methods for their production, inhibitors of binding of IL-13 and its receptor and methods for their identification are also disclosed....

20060177901 - Flt4 (vegfr-3) as a target for tumor imaging and anti-tumor therapy: The present invention provide purified Flt4 receptor tyrosine kinase polypeptides and fragments thereof, polynucleotides encoding such polypeptides, antibodies that specifically bind such polypeptides, and uses therefor....

20060177906 - Igf-i responsive gene and use thereof: A protein encoded by a gene comprises nucleic acid sequence SEQ ID No. 1, 2, 3, 4, 5, 6 or a derivative or mutant or fragment or variant or peptide thereof. The protein promotes the attachment and modulates the motility and invasion capability of cells....

20060177907 - Methods and materials for characterizing and modulating interaction between heregulin and her3: The disclosure provided herein identifies and characterizes the domain in HER3 receptor that interacts with heregulin ligand. Typical embodiments of the invention disclosed herein include HER3 variant polypeptides having amino acid sequences which differ from the native HER3 polypeptide sequence and which have altered affinities for heregulin. Also disclosed herein...

20060177895 - Methods for enhancing expression of recombinant proteins: The present invention provides two novel E. coli synthetic promoters, Syn 1 and Syn 2. Syn 1 and Syn 2 are two tight regulated synthetic promoters that control uninduced, leaky expression of proteins that are toxic and interfere with production of recombinant protein from an E. coli expression system....

20060177898 - Methods for producing modified glycoproteins: Cell lines having genetically modified glycosylation pathways that allow them to carry out a sequence of enzymatic reactions, which mimic the processing of glycoproteins in humans, have been developed. Recombinant proteins expressed in these engineered hosts yield glycoproteins more similar, if not substantially identical, to their human counterparts. The lower...

20060177903 - Polynucleotide encoding a novel human g-protein coupled receptor variant of hm74, hgprbmy74: The present invention provides novel polynucleotides encoding HGPRBMY74 polypeptides, fragments and homologues thereof. Also provided are vectors, host cells, antibodies, and recombinant and synthetic methods for producing said polypeptides. The invention further relates to diagnostic and therapeutic methods for applying these novel HGPRBMY74 polypeptides to the diagnosis, treatment, and/or prevention...

20060177897 - Pr1550 polypeptides and nucleic acids encoding the same: The present invention is directed to novel polypeptides and to nucleic acid molecules encoding those polypeptides. Also provided herein are vectors and host cells comprising those nucleic acid sequences, chimeric polypeptide molecules comprising the polypeptides of the present invention fused to heterologous polypeptide sequences, antibodies which bind to the polypeptides...

20060177894 - Protein knobs: Compositions containing a knob attached to a specific site of a protein and methods of producing and using these compositions are disclosed. The compositions comprise a knob, a tail portion and a protein portion. The protein portion contains a substituted cysteine residue at the desired location of labeling. The tail...

20060177904 - Secreted proteins and polynucleotides encoding them: Novel polynucleotides and the proteins encoded thereby are disclosed....

20060177905 - Udp-glucosyltransferases: This invention pertains to nucleic acid fragments encoding plant glucosyltransferases, heretofore undescribed, that exhibit catalytic activity with p-hydroxybenzoic acid (pHBA) as a substrate and only attach glucose to the aromatic carboxyl group of pHBA, to form the pHBA glucose ester. These enzymes have potential applications both in vitro and in...

20060177908 - Il-2 transmembrane construct: Compounds, genetic constructs, and cancer treatment methods are provided. Expression vectors were designed to express fusion genes including hIL-2 with a Fcε-γ transmembrane anchor derived from a subunit of the FC epsilon receptor. mRNA and the IL-2tm fusion protein was expressed in transfected RD995 tumor cells. Expression of the IL-2tm...

20060177909 - Preparation of immunoglobulin: The invention relates to a method for producing an immunoglobulin G preparation. In the method of blood fraction containing immunoglobulin is treated 60-72 hours with mild pepsin at a pH of about 4.4, treated by the S/D virus activation method and cleared of chemicals, pepsin and decomposition products. In addition,...

20060177911 - Delta 4,5 glycuronidase and methods of cleaving therewith: The invention relates to Δ4,5 glycuronidase, related compositions, and methods of use thereof....

20060177910 - Delta 4,5 glycuronidase and methods of hydrolyzing therewith: The invention relates to Δ4,5 glycuronidase, related compositions, and methods of use thereof....

20060177912 - Nucleotide sequences which code for the dep34 gene: The invention relates to an isolated polynucleotide having a polynucleotide sequence which codes for the dep34 gene, and a host-vector system having a coryneform host bacterium in which the dep34 gene is present in attenuated form and a vector which carries at least the dep34 gene according to SEQ ID...

20060177913 - Process for enantioselective enzymatic reduction of keto compounds: Chiral secondary alcohols may be produced enzymatically in high space-time yields while minimizing enzyme use, by reducing a keto compound in an aqueous reaction medium containing water, reducing agent, alcohol dehydrogenase and coenzyme, extracting the secondary alcohol formed by means of a further phase containing a water-immiscible organic solvent, and...

20060177914 - Coupled cofactor-dependent enzymatic reaction system: The present application relates to a reaction system in which chemically valuable compounds can be obtained in high enantiomer purities with the aid of a coupled enzymatically operating transformation process. The coupled enzymatically operating reaction system in this context comprises an enzymatic transformation reaction which proceeds with the consumption of...

20060177915 - Method for the production of glycerol by recombinant organisms: Recombinant organisms are provided comprising genes encoding a glycerol-3-phosphate dehydrogenase and/or a glycerol-3-phosphatase activity useful for the production of glycerol from a variety of carbon substrates. The organisms further contain disruptions in the endogenous genes encoding proteins having glycerol kinase and glycerol dehydrogenase activities....

20060177916 - Ethanol production from citrus processing waste: Processes for producing ethanol from citrus waste by reducing the concentration of limonene in citrus waste to allow fermentation are disclosed. In one embodiment a slurry of ground citrus waste 1 is partially hydrolyzed by heating using a jet cooker 2 and then injected into a flash tank 4 to...

20060177917 - Process for the production of cellulolytic and hemicellulolytic enzymes using distillation residues from the ethanolic fermentation of enzymatic hydrolyzates of (ligno)cellulosic materials: A process for producing cellulolytic and/or hemicellulolytic enzymes uses the residue from the ethanolic fermentation of enzymatic hydrolyzates of cellulosic or ligno-cellulosic materials. This process may be integrated into a process for the production of ethanol from cellulosic or ligno-cellulosic materials which comprises the following steps: 1) chemical and/or physical...

20060177918 - Compositions, methods and kits for real-time enzyme assays using charged molecules: Compositions, methods and kits useful for, among other things, detecting, quantifying and/or characterizing enzymes....

20060177919 - Process for the preparation of (s) - or (r) -3,3,3- trifluoro-2-hydroxy-2-methylpropionic acid: m

20060177920 - Process for the heterotrophic production of microbial products with high concentrations of omega-3 highly unsaturated fatty acids: Disclosed is a process for growing the microflora Thraustochytrium, Schizochytrium, and mixtures thereof, which includes the growing of the microflora in fermentation medium containing non-chloride containing sodium salts, in particular sodium sulfate. In a preferred embodiment of the present invention, the process produces microfloravhaving a cell aggregate size useful for...

20060177921 - Method for removing unsaturated uronic acid in chemical pulp for papermaking: The present invention provides a method for removing unsaturated uronic acid contained in chemical pulp for papermaking, characterized by adding glucuronidase. According to the preferred embodiment of the present invention, in an ECF or TCF bleaching method using no molecular chlorine, unsaturated uronic acid can be reduced while controlling a...

20060177922 - Environmental control incubator with removable drawer and robot: An incubator for storing micro-plates or micro-tubes comprises a handling robot positioned between shelves or drawers containing micro-titer plates or other containers useful for biological based reactions. The advantages of this configuration are the ultimate compactness of the system and increased speed or reliability achieved with more than one robot...

20060177924 - Seeding cells on porous supports: The present invention generally relates to a method for seeding cells on to a support. In particular, the method relates to a method for seeding cells onto a porous hydrophobic support. The method utilizes centrifugal forces to uniformly guide cell seeding into the support with no loss in viability....

20060177923 - Spatially-defined modification of fresh tissue using covalent chemistry: Methods for modification of tissue using covalent chemistry. Tissue can be modified through direct alkylation, reduction followed by alkylation, or oxidation followed by condensation to covalently attach small organic molecules or appropriately modified proteins. The modification can be spatially limited to desired regions of the tissue surface....

20060177925 - Kidney derived stem cells and methods for their isolation, differentiation and use: The invention relates generally to methods for isolation and culture of kidney stem cells, cells isolated by the methods, and therapeutic uses for those cells....

20060177926 - Common marmoset embryonic stem cell lines: The present invention relates to novel common marmoset (Callithrix jacchus) embryonic stem cell cultures and cell lines, and their use. The common marmoset embryonic cells of the present invention (i) are capable of prolonged undifferentiated proliferation in vitro, (ii) maintain, during prolonged culture, a karyotype in which all the chromosomal...

20060177927 - Method for growth of human conjunctival tissue equivalents for research, clinical ocular surface transplantation and tissue engineering: The invention relates to materials and methods for producing equivalents of tissues of the ocular surface, especially conjunctival tissue equivalents. The method of the invention involves biopsy of an appropriate tissue, primary culture in a certain medium, proliferative culture in a second medium, and differentiative culture in a third medium....

20060177928 - Method of generating neural stem cells: The present invention relates to a method of generating neural stem cells from mesodermal cells. The invention further provides a cell population which has neural stem cell-like characteristics. The neural stem cells prepared according to the invention are useful in the treatment of neurodegenerative disorders such as Alzheimer's disease and...

20060177929 - Regulation of self-renewal in stem cells: Disclosed are methods for regulating the self-renewal capacity of stem cells such as, but not limited to, primitive hematopoietic stem cells (HSCs) (both mouse and human) by modulating the function and/or activity of target factors that are controlled or altered in their regulation by AML1-ETO expression in stem cells such...

20060177930 - Method to enhance biodegradation of sulfonated aliphatic-aromatic co-polyesters by addition of a microbial consortium: A microbial consortium for the biodegradation of sulfonated aliphatic-aromatic co-polyesters having greater than 60 mol percent aromatic acid content based on the total acid content of the co-polyester, created by applying selective pressure. Methods to biodegrade sulfonated aliphatic-aromatic co-polyesters using microbial consortium SPDC-1 are provided....

20060177931 - Apparatus and method for co-culturing of cells: A co-culture apparatus and method for, among other things, converting a progenitor cell line into a target cell line. The apparatus comprises an exterior receptacle, a removable cartridge that fits into the exterior receptacle, and a closure member to seal the apparatus. The invention is also directed to a method...

20060177932 - Expansion agents for stem cells: The present invention relates to a method and substance for maintaining the pluripotency and self-replication ability of a stem cell, such as a hematopoietic stem cell, while keeping it undifferentiated. Specifically, the present invention provides a composition for maintaining the expansion or pluripotency of a stem cell, comprising active STAT5,...

20060177933 - Expression of apoa-1 and variants thereof using spliceosome mediated rna trans-splicing: The present invention provides methods and compositions for generating novel nucleic acid molecules through targeted spliceosome mediated RNA trans-splicing that result in expression of an apoA-1 variant, the preferred embodiment referred to herein as the apoA-1 Milano variant. The compositions of the invention include pre-trans-splicing molecules (PTMs) designed to interact...

20060177934 - Lentiviral ltr-deleted vector: A vector capable of transducing non-dividing and/or slowly dividing cells is provided, wherein the vector is a lentiviral LTR-deleted vector. Also provided is a method for producing a protein of interest in a non-dividing or slowly dividing cell by transducing the cell with a lentiviral LTR-deleted vector and expressing the...

20060172279 - Cell-based sensing: biological transduction of chemical stimuli to electrical signals (nose-on-a-chip): Methods and compositions for the reliable detection of chemical stimuli using a “nose-on-a-chip” are presented. The invention uses cells sensitive to chemical stimuli and detects and processes the signals given by the cells upon contact with chemical stimuli....

20060172277 - Method for determining the influence of a test substance on the heart activity of a vertebrate: In a method for determining the influence of a test substance on the heart activity of a vertebrate the following steps are performed: a) preparation of a culture of spontaneously active heart cells of the vertebrate, b) extracellular measurement of electrophysiological data of the heart cells from step a), c)...

20060172280 - System for monitoring cell motility in real-time: The invention relates to devices, devices for arraying biomolecules, including cells, methods for arraying biomolecules, assays for monitoring cellular movement, and systems for monitoring cellular movement. The devices include a support; a first layer configured to be placed in fluid-tight contact with the support, the first layer having an upper...

20060172278 - Target activated microtransfer: A device for performing target activated transfer that includes a mounting surface for mounting a tissue sample; and a light source positioned to substantially uniformly irradiate both stained and unstained regions of the tissue sample with light energy that activates the reagent to selectively adhere the stained regions to a...

20060172286 - Diagnosis method of alcholic or non-alcoholic steato-hepatitis using biochemical markers: The present invention is drawn to a new diagnosis method for detecting the extent of alcoholic or non-alcoholic steato-hepatitis in a patient, in particular in a patient suffering from a disease involving alcoholic or non-alcoholic steato-hepatitis or who already had a positive diagnosis test of liver fibrosis and/or presence of...

20060172284 - Highly orthogonal universal sequences for use in nucleic acid assays: The invention provides a set of highly orthogonal six-code universal sequences for use in bDNA singleplex and multiplex nucleic acid hybridization assays. The six-code orthogonal sequences do not cross-hybridize and thus, minimize or eliminate the 3-mer cross-hybridization inherent in the second and third generation bDNA assays. The highly orthogonal universal...

20060172285 - Hpv e6, e7 mrna assay and methods of use thereof: Provided is an HPV E6, E7 mRNA assay, referenced herein as the “In Cell HPV Assay,” that is capable of sensitive and specific detection of normal cervical cells undergoing malignant transformation as well as abnormal cervical cells with pre-malignant or malignant lesions. The In Cell HPV Assay identifies HPV E6,...

20060172283 - Method and apparatus to separate molecules according to their mobilities: A method and apparatus for separating molecules comprises placing different kinds of molecular species onto a probe; and introducing an electric field between the probe and a surface in proximity with the probe so that the different kinds of molecular species may be separated, wherein the different kinds of molecular...

20060172281 - Method for identifying and for extracting endotoxin: The invention relates to a method for identifying endotoxins for eliminating said endotoxins from a sample, with the aid of bacteriophage tail proteins....

20060172288 - Methods for the treatment of cellular proliferative disorders: The present invention relates to methods of identifying the susceptibility of cells to reovirus infection by measuring constitutive ras-MAP signaling. The invention also pertains to methods using reovirus for the treatment of cellular proliferative disorders, and particularly cellular proliferative disorders wherein the proliferating cells exhibit constitutive MAPK phosphorylation, in mammals....

20060172287 - Murine calicivirus: The invention disclosed herein relates to a newly discovered murine norovirus, and compositions and methods related thereto....

20060172282 - Peptide templates for nanoparticle synthesis obtained through pcr-driven phage display method: A method is provided for identifying and isolating peptides capable of binding of inorganic materials such as silica, silver, germanium, cobalt, iron, or oxides thereof, or other materials on a nanometric scale such as carbon nanotubes, using a combinatorial phage display peptide library and a polymerase-chain reaction (PCR) step to...

20060172309 - 5-methylcytosine detection, compositions and methods therefor: Compositions and methods for detecting 5-methylcytosine in a nucleic acid are disclosed. A 5-methylcytosine discriminator, which is a deoxyribonucleosidetriphosphate comprising a cytosine-pairing moiety such as a guanosine and a moiety which hinders hydrogen bonding between the cytosine-pairing moiety and a 5-methylcytosine is described. The discriminator is able to base pair...

20060172302 - Assay method: The invention provides an assay method for detecting fungal infection of soil or vegetables by pathogenic fungal species, in particular M. acerina, F. carotae and Pythium species, said method comprising: obtaining a sample of soil or vegetable; treating said sample to lyse fungal cells therein; using an oligonucleotide primer pair,...

20060172335 - Assay method for group transfer reactions: The present invention relates to methods for detecting, quantifying and high throughput screening of donor-products and the catalytic activities generating the donor-products in group-transfer reactions catalyzed by adenosine triphosphatase (ATPase) or guanine triphosphatase (GTPase). The invention further provides immunoassays, antibodies and kits that may be used to practice the methods...

20060172330 - Categorization of microbial communities: The present disclosure provides embodiments of a method for characterizing microbial populations. Exemplified by methods for characterizing microbiota in vaginal samples, the methods provided herein are widely applicable to the characterization of microbial communities. Also provided are probiotic regimens and methods for selecting appropriate probiotic regimens based on the normal...

20060172289 - Combinatorial oligonucleotide pcr: The present invention is directed to a method of detecting gene expression and analysis of both known and unknown genes. In the method utilizing combinatorial oligonucleotide PCR™, no single molecular species of DNA gives rise to more than one fragment in a collection of products which are subsequently amplified and...

20060172333 - Compositions and methods for terminating a sequencing reaction at a specific location in a target dna template: Compositions and methods for sequencing a template polynucleotide comprising a sequence of interest are provided herein. The compositions and methods employ at least one blocking probe that is designed to bind in a sequence-specific manner to a blocking sequence such that primer extension beyond the site where the blocking probe...

20060172298 - Denaturat stable and/or protease resistant, chaperone-like oligomeric proteins, polynucleotides encoding same, their uses and methods of increasing a specific activity thereof: Novel denaturant-stable, protease resistant, homo-oligomeric proteins, also referred to herein as stable proteins (SPs), having chaperone-like activity; methods of production and purification of SPs; nucleic acids encoding SPs; methods of isolating nucleic acids encoding SPs; antibodies recognizing SPs; the use of SPs for stabilizing, refolding, repairing, preventing aggregation and de-aggregating...

20060172310 - Detection and quantification process for more than one nucleic acid in library: This invention provides novel compositions and processes for analyte detection, quantification and amplification. Nucleic acid arrays and libraries of analytes are usefully incorporated into such compositions and processes. Universal detection elements, signaling entities and the like are employed to detect and if necessary or desirable, to quantify analytes. Amplification of...

20060172294 - Detection of epigenetic abnormalities and diagnostic method based thereon: The present invention provides a method of detecting an epigenetic abnormality associated with a disease. The method comprises identifying, within a eukaryotic genome, a locus having a hypomethylated sequence specific for the disease and an endogenous multi-copy DNA element. The method can also comprise separate steps of identifying a disease-specific...

20060172321 - Detection of extracellular tumor-associated nucleic acid in blood plasma or serum using nucleic acid amplification assays: This invention relates to detection of specific extracellular nucleic acid in plasma or serum fractions of human or animal blood associated with neoplastic or proliferative disease. Specifically, the invention relates to detection of nucleic acid derived from mutant oncogenes or other tumor-associated DNA, and to those methods of detecting and...

20060172325 - Detection of nucleic acids: Disclosed herein are oligonucleotide microarrays, wherein the microarrays comprise a plurality of target-specific oligonucleotide probes, including both sense and antisense probe pairs for each target nucleic acid. Such arrays are useful for high throughput detection of target nucleic acids in a sample, particularly when coupled to multiplex PCR. Also disclosed...

20060172329 - Dna markers for cattle growth: The invention provides methods for identifying a genetic polymorphism associated with increasing weaning weight in progeny of female beef cattle comprising the polymorphism. Genetic marker-assisted selection methods provided by the invention allow avoidance of potentially costly phenotypic testing and inaccuracies associated with traditional breeding schemes and improvement of beef cattle...

20060172297 - Enhancers specific to motor nerve cells/sensory nerve cells: This invention provides a method for improving gene expression efficiency specifically in motor neurons and/or in sensory neurons and an enhancer consisting of the following DNA (a), (b), or (c): (a) DNA consisting of the nucleotide sequence as shown in any one of SEQ ID NOs: 1 to 4; (b)...

20060172295 - Genes associated with schizophrenia adhd and bipolar disorders: Disclosed are methods for diagnosing, monitoring the progression of, and treating schizophrenia, bipolar disorder, and/or ADHD based upon genes that are differentially expressed in said disorders at baseline, or at different timepoints following an acute stress exposure. Also disclosed are methods for identifying agents useful in the treatment of schizophrenia,...

20060172326 - Hmg coenzyme a reductase inhibitors affect the mitochondrial dna content of cells: Statins are widely used for the treatment of high cholesterol levels. In the present invention, the effect of statins on mitochondrial nucleic acid and the activation state of mitochondria is used in methods for determining whether a subject is at risk of developing side effects of statin treatment, methods for...

20060172323 - Identification of compounds that modify transcriptional responses to hypoxia: The invention provides methods for identifying compounds that can be used to modify transcriptional responses to hypoxia, and use of such compounds in therapeutic and diagnostic methods....

20060172331 - Isolation of nucleic acid from stool samples and other biological materials which are rich in inhibitors: The invention relates to a method for the stabilization, purification or/and isolation of nucleic acids from material samples, in particular, stool samples, which can contain impurities and inhibitors or interfering substances. The invention further relates to a reagent kit for carrying out said method. The basis of the invention is,...

20060172308 - Labeling reagents and labeled targets comprising nonmetallic porphyrins: This invention provides for labeling reagents, labeled targets and processes for preparing labeling reagents. The labeling reagents can take the form of cyanine dyes, xanthene dyes, porphyrin dyes, coumarin dyes or composite dyes. These labeling reagents are useful for labeling probes or targets, including nucleic acids and proteins. These reagents...

20060172319 - Mass tags for quantitative analyses: This invention pertains to methods, mixtures, kits and/or compositions for the determination of analytes by mass analysis using unique labeling reagents or sets of unique labeling reagents. The labeling reagents can be isomeric or isobaric and can be used to produce mixtures suitable for multiplex analysis of the labeled analytes....

20060172334 - Method for detecting adverse reaction susceptibility to an hmg coa reductase inhibitor: The present invention provides a method for detecting adverse reaction susceptibility and/or severity risk to an HMG CoA reductase inhibitor by determining the genotype of a CYP3A gene of a subject....

20060172306 - Method for detection of mutations in dna: A method for the detection in a given DNA sequence of DNA mutations, single nucleotide polymorphisms, and insertions and deletions, said method comprising the step of a) producing replicate(s) with an engineered polymerase of said given DNA sequence with part or all of at least one of the four natural...

20060172305 - Method for determining susceptibility of tumor to treatment with anti-neoplastic agent: The level of one or more glucose transporters in a sample containing cancer cells is measured and compared to a reference value to determine whether the cancer is susceptible to treatment with an anti-cancer agent comprising glucose or glucose analog that is transported into the cancer by a glucose transporter....

20060172300 - Method for identifying bhs-specific proteins and fragments thereof: The invention relates to a method for identifying the presence of BBB-specific protein/fragment in endothelial cells of brain capillaries, characterized in that a) endothelial cells of brain capillaries freshly isolated from brain are conventionally pre-purified by enzymatic digestion, b) the digest obtained in step a) is treated with a lysis...

20060172304 - Method for modulating epithelial stem cell lineage: The present invention relates to methods of modulating epithelial stem cell lineage by regulating the expression of Lef1 or a BMP inhibitor and/or the stability of β-catenin or the expression of a Wnt; regulating the expression or activity of GATA-3; or regulating BMPR1A activity either at the level of receptor...

20060172303 - Method for the separation of cell fractions: The present invention relates to a method for the separation of cells, in particular for the preparation of samples in tumor diagnostics. In particular, the present invention relates to a method for sample preparation for the detection of tumor cells of solid tumors in the course of the diagnosis for...

20060172307 - Method of nucleotide identification using an off-switch through proofreading 3' exonuclease-resistant modified primers by polymerases with 3' exonuclease activity: Methodology for a high fidelity polymerase-mediated primer extension for use in genotyping is provided. The primer extensions are carried out with polymerase having 3′ to 5′ exonuclease activity and primers with their 3′ modification of being exonuclease-resistant. The primers are designed to have their 3′ termini: 1. complementary to nucleotide...

20060172328 - Methods and compositions for correcting misincorporation in a nucleic acid synthesis reaction: The invention provides methods for correcting misincorporation of a nucleotide in a primer during a sequencing-by-synthesis reaction by using both a polymerase substantially lacking in exonuclease activity and an enzyme, preferably a polymerase, having exonuclease activity....

20060172313 - Methods and compositions for improving fidelity in a nucleic acid synthesis reaction: The invention provides methods and compositions for improving the fidelity of a sequencing-by-synthesis reaction by using a nucleotide derivative that forms a hydrogen bond with a complementary nucleotide on a template, but fails to form a phosphodiester bond with the 3′ hydroxyl group of a primer under conditions otherwise suitable...

20060172315 - Methods for staining cells for identification and sorting: The present invention provides novel methods of cell staining, such as bovine sperm, using electroporation or osmolality treatments at viability-enhancing temperatures. Furthermore, methods of highly efficient cell sorting that are especially suitable in sorting bovine sperm using novel cell staining procedures are also provided....

20060172299 - Methods for the synthesis of mucleic acids: The invention relates to a method for the synthesis of nucleic acids, where incubation of a polymerase, a nucleic acid that can be used as a template for the polymerase, NTPs and Mn2+ is performed under conditions that enable the synthesis of a nucleic acid strand and the method is...

20060172324 - Methods of genotyping using differences in melting temperature: The present invention relates to the identification of a particular nucleotide polymorphism in a nucleic acid sample in a single reaction utilizing oligonucleotide primers with different melting temperature characteristics....

20060172320 - Modular aptametric sensors without covalently attached fluorophores: Modular aptameric sensors, transduce recognition events into fluorescence changes through allosteric regulation of non-covalent interactions with a fluorophore. These sensors consist of: (a) a reporting domain, which signals the binding event of an analyte through binding to a fluorophore; (b) a recognition domain, which binds the analyte; and (c) a...

20060172312 - Multi-component inhibitors of nucleic acid polymerases: The present invention provides multi-component inhibitors of nucleic acid polymerases, methods of making, and methods of using same. One component of the multi-component inhibitor is a molecule that binds to a polymerase (i.e., a polymerase-binding molecule (PBM)), but does not thereby substantially inhibit its polymerase activity. Another component is a...

20060172292 - Multivariate random search method with multiple starts and early stop for identification of differentially expressed genes based on microarray data: The present invention provides multivariate methods for analyzing microarray gene expression data of high dimensional space and thereby identifying differentially expressed genes. The methods of this invention provide a random search procedure with multiple starts and early stop. Larger sets of differentially expressed genes may be identified using the methods...

20060172322 - Mutations in the c7orf11 (ttdn1) gene causative of non-photosensitive trichothiodystrophy: The invention is the demonstration that mutations of the C7orf11 nucleic acid sequence leads to the development of non-photosensitive trichothiodystrophy. The invention comprises methods of screening for and detection of non-photosensitive TTD carriers, prenatal screening and diagnosis, diagnosis of non-photosensitive TTD, drug and gene therapy, manufacture of the protein, and...

20060172296 - Novel gene associated with rheumatoid arthritis: It is disclosed that novel polypeptides which has enhanced expression levels in the synovial tissues of human chronic rheumatoid arthritis (RA) patients compared with the synovial tissues of human osteoarthritis (OA) patients, polynucleotides encoding the polypeptides, vectors comprising the polynucleotides, transformant cells comprising the vectors and an antibody against the...

20060172293 - Novel method of assaying nucleic acid using labeled nucleotide: A novel method is provided to assay at least one nucleic acid. According to this method, a nucleic acid polymerization reaction is conducted in a nucleic acid polymerization reaction system, which contains (A) the at least one nucleic acid as a template, (B) at least one nucleotide monomer labeled with...

20060172311 - Novel nucleotide and amino acid sequences, and assays and methods of use thereof for diagnosis of cardiac disease: Novel markers for cardiac disease that are both sensitive and accurate. These markers are differentially and/or specifically expressed in cardiac tissue, as opposed to other types of tissues, optionally and preferably including muscle tissue. The measurement of these markers, alone or in combination, in patient samples provides information that the...

20060172290 - Nucleic acids coding for a protein interacting with a porin channel: The invention relates to an isolated nucleic acid coding for at least a partial sequence of a protein kinase of the mitogenic signaling cascade, in particular c-raf, wherein the partial sequence interacts with VDAC or BAX channels in mitochondrial membranes, or with a nucleic acid hybridizing said nucleic acid or...

20060172336 - Pcr and hybridization methods utilizing electrostatic transportation and devices therefor: A biological sample (droplets containing DNA and primer) (9) is prepared in a chemically inert liquid layer (8), is electrostatically transported by the action of an electrostatic transportation plate (1) with electrostatic transportation electrodes (2) and is heated at a predetermined position of the electrostatic transportation plate (1), thus carrying...

20060172291 - Polymorphisms in the human gene for cytochrome p450 polypeptide 2c8 and their use in diagnostic and therapeutic applications: The present invention relates to a polymorphic CYP2C8-polynucleotide. Moreover, the invention relates to genes or vectors comprising the polynucleotides of the invention and to a host cell genetically engineered with the polynucleotide or gene of the invention. Further, the invention relates to methods for producing molecular variant polypeptides or fragments...

20060172316 - Quality value for nucleic acids: A method of assessing the quality of an amplified nucleic acid sample is described. The method includes (a) separating the amplified nucleic acid sample to obtain a separation profile; (b) determining a first component of said separation profile corresponding to a first nucleic acid fraction; (c) determining a second component...

20060172314 - Quantification of amplified nucleic acids: The invention relates to methods and kits for quantification of amplified nucleic acids, such as genomic DNA. The methods and kits can be used to assess bias in an amplification procedure such as a whole genome amplification procedure. In one aspect, the method comprises performing an enzyme-based amplification procedure and...

20060172318 - Reagentless and reusable biosensors with tunable differential binding affinities and methods of making: The biosensor comprises a modular biorecognition element and a modular flexible arm element. The biorecognition element and the flexible arm element are each labeled with a signaling element. The flexible arm contains an analog of an analyte of interest that binds with the biorecognition element, bringing the two signaling elements...

20060172317 - Rna labeling method: Methods are described in which a sample containing RNA is contacted with an enzyme having an RNA ligation activity in the presence of a labeled substrate to provide labeled RNA. Methods of performing an array analysis of a labeled RNA sample are also described....

20060172301 - Secreted acid phosphatse (sapm) is present only in pathogenic mycobacteria and expressed selectively at phagosomal ph: The invention relates to an isolated DNA construct, comprising a mycobacterium secreted acid phosphatase promoter or promoter fragment which is inducible by low pH. This invention further relates to diagnostic methods and vaccines for treatment or prophylaxis of pathogenic mycobacterial disease or infection. This invention also provides for methods of...

20060172327 - Sequencing of surface immobilized polymers utilizing microflourescence detection: Means for simultaneous parallel sequence analysis of a large number of biological polymer macromolecules. Apparatus and methods may use fluorescent labels in repetitive chemistry to determine terminal monomers on solid phase immobilized polymers. Reagents which specifically recognize terminal monomers are used to label polymers at defined positions on a solid...

20060172332 - Use of protective natural polymers to isolate and preserve biological material: Compositions and methods for the reversible preservation of biological samples are provided. The compositions include natural polymers such as pullulan or acacia gum, including derivations and modifications thereof, which are usefull as a reversible preservation solution. A method is provided for using pullulan or Acacia Gum to isolate and reversibly...

20060172342 - Antibody assay methods to assess risk for tia/stroke: A methods, kits and compositions for diagnosing a central nervous system disorder, particularly transient ischemic attack or stroke, comprising measuring the level of NR2A and/or NR2B NMDA receptor or fragment thereof, in a biological sample from a human subject, and optionally measuring other biomarkers such as homocysteine and glutamate. The...

20060172341 - Assay methods for immediate diagnosis of cause of stroke: A methods, kits and, compositions for diagnosing a central nervous system disorder, particularly transient ischemic attack or stroke, comprising measuring the level of NR2A and/or NR2B NMDA receptor or fragment thereof, in a biological sample from a human subject, and optionally measuring other biomarkers such as homocysteine and glutamate. The...

20060172338 - Method for diagnosing multiple sclerosis: Disclosed is a method for diagnosing multiple sclerosis and more particularly to a method for diagnosing multiple sclerosis by measuring levels of antibodies to glycans in a biological sample....

20060172340 - Multi-array, multi-specific electrochemiluminescence testing: Materials and methods are provided for producing patterned multi-array, multi-specific surfaces for use in diagnostics. The invention provides for electrochemiluminescence methods for detecting or measuring an analyte of interest. It also provides for novel electrodes for ECL assays. Materials and methods are provided for the chemical and/or physical control of...

20060172343 - Novel vegf-like factors: A novel human gene having a significant homology with a VEGF-C gene, a member of the VEGF family, has been isolated by the PCR method using primers designed based on the sequence of EST that is assumed to be homologous with the C-termial region of the VEGF-C gene. Mouse and...

20060172339 - Particle-based multiplex assay for identifying glycosylation: The present invention provides systems, methods and kits for performing multiplexed assays for glycoproteins using encoded particles as supports for glycoprotein specific binding agents....

20060172337 - Screening molecules with anti-prion activity: kits, methods and screened molecules: The invention concerns screening molecules with anti-prion activity. More particularly, it concerns kits for screening molecules with anti-prion activity characterized in that they comprise in combination a [PSI+], phenotype yeast, an antibiogram and an agent for purifying prions at sub-efficient doses, said yeast including the adel-14 allele of the ADE1...

20060172346 - Methods of designing and producing novel compounds having improved binding affinity for cd154 or other trimeric proteins: The present invention relates to methods for designing and producing novel CD40:CD154 binding interrupter compounds and methods using these compounds to treat conditions associated with inappropriate CD154 activation in a subject. This invention also relates to novel methods for screening candidate compounds for the properties of specifically binding CD154 and...

20060172350 - Adam-9 modulators: The invention provides the identification and characterization of a disease and cancer-associated antigen, KID24. The invention also provides modulators of KID24, including a family of monoclonal antibodies that bind to antigen KID24, and methods of diagnosing and treating various human cancers and diseases associated with KID24....

20060172349 - Luca2 and antibodies that bind thereto: The invention provides the identification and characterization of disease and cancer-associated antigen, LUCA2. The invention also provides a family of monoclonal antibodies that bind to antigen LUCA2, methods of diagnosing and treating various human cancers and diseases that express LUCA2....

20060172347 - Method of diagnosis, treatment and useful agents for conditions characterised by modulation in the level of activin ssc: The present invention relates generally to a method of diagnosing, predicting or monitoring the development or progress of a condition characterised by modulation in the level of activin expression and, more particularly, a method of diagnosing, predicting or monitoring the development or progress of a condition characterised by modulation in...

20060172352 - Nuclear matrix proteins: Nuclear matrix proteins (NMP) which are characterized by a defined expression in tissue are provided. These NMPs are useful markers in diagnosing and monitoring the stage of malignancy of a cell and treating cell proliferative disorders associated with the NMP. Also provided are substantially purified polypeptides and nucleotide sequences encoding...

20060172351 - Reagent for an immunoassay: The present invention relates to (1) A reagent for an immunoassay of a target substance existing in a free form and a bound form in a specimen, comprising a latex 1 which is immobilized with a monoclonal antibody 1 for the target substance, and a latex 2 which has a...

20060172348 - Use of neuronal apoptosis inhibitor protein (naip): The invention provides a novel NAIP nucleic and sequences. Also provided are anti-NAIP antibodies and methods for modulating apoptosis and detecting compounds which modulate apoptosis....

20060172353 - Diagnosis of candidiasis and candidemia or invasive candida infection: The use of an antibody to a C albicans cell wall antigen or to a solubilized phosphopeptidomannan (PPM) fraction of the cell wall of C albicans, or preferably a combination of an IgG2 antibody to a phosphopeptidomannan (PPM) fraction of the cell wall of C albicans, and an IgG1 or...

20060172354 - Novel proteins in enteroaggregative escherichia coli (eaec) useful for diagnosis and therapy of eaec infections: Novel proteins and their corresponding nucleotide sequences in enteroaggregative Escherichia coli (EAEC) are provided. In particular, Aap and the five gene cluster (aat) of the AA probe region of the pAA plasmid of EAEC 042 have been identified, sequenced, and further characterized. The use of these novel proteins and their...

20060172355 - Novel proteins in enteroaggregative escherichia coli (eaec) useful for diagnosis and therapy of eaec infections: Novel proteins and their corresponding nucleotide sequences in enteroaggregative Escherichia coli (EAEC) are provided. In particular, Aap and the five gene cluster (aat) of the AA probe region of the pAA plasmid of EAEC 042 have been identified, sequenced, and further characterized. The use of these novel proteins and their...

20060172344 - Single chain antibody and use thereof: The present invention provides a single chain antibody that retains its original specific binding activity with an antigen, and a labeled single chain antibody in which a labeling substance is bound to the single chain antibody. Specifically, the labeled single chain antibody of the present invention can be produced by...

20060172345 - Method for detecting low levels of a fusion protein: The invention relates to the detection of, among others, tumor-specific fusion proteins. Provided is a method for detecting a fusion protein in a sample, the fusion protein comprising an amino-terminal fragment and a carboxy-terminal fragment that each correspond to a native protein. The method comprises contacting the sample with at...

20060172357 - Detecting human antibodies in non-human serum: The present invention provides for the quantification of a human, human-chimeric, humanized antibody, or a fragment thereof, without the necessity of using a target-specific molecule. More particularly, the invention relates to a quantification assay that includes the step of blocking non-specific binding sites of a capture reagent with a blocking...

20060172356 - Immunoassays for lamotrigine: Generally, the present invention relates to lamotrigine analogs that have substituents at the triazine 3-position and on the benzene 4-position and 5-position. The lamotrigine analogs can include immunogenic moieties that can be used to prepare anti-lamotrigine antibodies, or antigenic moieties that can be used in immunodiagnostic assays for lamotrigine. Also,...

20060172359 - Method of preparing cow brain homogenate: An assay method is disclosed which isolates and detects the presence of a disease related conformation of a protein (e.g., PrPSc) present in a sample also containing the non-disease related conformation of the protein (e.g., PrPSc). The sample is treated (e.g., contacted with protease) in a manner which hydrolyzes the...

20060172358 - Novel chemiluminescent compounds and their use: The present invention relates to novel chemiluminescent compounds, to a method for synthesizing these compounds, to derivatives and conjugates comprising these compounds, to the use of these compounds or conjugates thereof in chemiluminescence based assays, especially in immunoassays....

20060172361 - Assay for detection of translocase enzyme activity in drug screening: The invention provides a method for assaying the activity of the translocase enzyme involved in peptidoglycan biosynthesis in bacteria using scintillation proximity assay methodology. The method is suitable for high throughput screening of potential anti-bacterial drugs....

20060172362 - Methods and compositions for assaying homocysteine: This invention relates generally to the field of homocysteine detection. In particular, the invention provides a method for determining homocysteine presence or concentration in samples, which method comprises: contacting a sample containing or suspected of containing Hcy with a Hcy co-substrate and a Hcy converting enzyme in a Hcy conversion...

20060172360 - Mkk7 activation inhibitor: PAK4 and JIK, both of which bind to MKK7 and directly phosphorylate MKK7, were found in the present invention. The present invention provides an inhibitor of c-Jun phosphorylation caused by JNK3 and a method for inhibiting the same, and an agent for preventing and/or treating a disorder attributable to c-Jun...

20060172364 - Novel chromogenic substrates based on alizarin, their applications and formulations containing such substrates: An alizarin-based chromogenic substrate, a composition containing the substrate, and its use to detect the presence of an enzymatic activity. The substrate is particularly applicable in the field of biological diagnosis....

20060172363 - Peptide complexes containing phospholipase d: The present invention provides peptide complexes comprising phospholipase D and one or morephospholipase D-interacting peptides. The peptide complexes are useful in screening assays foridentifying compounds effective in modulating the peptide complexes and in treating and/or preventing diseases associated with phospholipase D and it's interacting partners. In addition, methods for screening...

20060172365 - Mass spectrometry: The present invention is concerned with methods for the de novo sequencing of polypeptides from data obtained from mass spectrometry devices, particularly from (MS)n devices....

20060172366 - Two-phase alocohol dehydrogenase-based coupled enzymatic reaction system: The present process relates to a coupled enzymatic reaction system which is implemented in a two-phase system consisting of organic phase and aqueous phase. The system operates with cofactor-dependent enzymes, the cofactor being continually regenerated enzymatically. The key enzyme is the alcohol dehydrogenase....

20060172367 - Fructosylamine oxidase: The present invention provides a fructosylamine oxidase which is obtainable by culturing Fusarium proliferatum, and purifying two types of fructosylamine oxidase (FAO) with different substrate specificities from the culture, and which is useful in the measurement of amadori compounds....

20060172368 - Cgt1 as an antifungal target: The invention provides CGT1 guanylyltransferase (CGT1) as a novel antifungal target, screening methods for CGT1 inhibitors and their use as antifungal compounds, pharmaceutical compositions containing them and their use in medicine....

20060172371 - Polydiacetylene-containing solid colorimetric and/or fluorescent detector, method for its preparation and uses thereof: The invention provides a colorimetric and/or fluorescent detector, which is a solid organic matrix, most preferably in the form of a gel, comprising polydiacetylene(s) and one or more lipids. Processes for preparing the detector and methods for using the same, including for the detection of microorganism suspected to be present...

20060172369 - Solvent for chromogenic substrate solution: The present invention relates to a non-toxic dipolar solvent for chromogenic substrate for detecting presence of lacZ gene and/or gene activity, which comprises a stabilizing amount of a solubilizing agent. The present invention also relates to a method for inducing lac operon in screening assay, comprising the step of contacting...

20060172370 - System and method for rapid detection and characterization of bacterial colonies using forward light scattering: A system and a method of detecting and characterizing a bacterial colony are presented in which the results are determined within about 48 hours. The bacterial colony is disposed on a substrate and placed between a laser and detector. Light from the laser impinges upon and is scattered by the...

20060172372 - Acc gene: The present invention relates to a gene useful in a process to increase the microbial production of carotenoids. The carotenoids astaxanthin is distributed in a wide variety of organisms such as animals, algae and microorganisms. It has a strong antioxidation property against reactive oxygen species. Astaxanthin is used as a...

20060172391 - Bmp-11 compositions: Purified BMP-11 proteins and processes for producing them are disclosed. Recombinant DNA molecules encoding the BMP-11 proteins are also disclosed. The proteins may be useful in regulating follicle stimulating hormone, such as for contraception. In addition, the proteins may be useful for the induction of bone, cartilage and/or other connective...

20060172389 - Canine erythropoietin gene and recombinant protein: One aspect of the present invention is an isolated nucleic acid molecule encoding canine erythropoietin. The present invention also relates to an isolated canine erythropoietin protein or polypeptide. Another aspect of the present invention is a method for providing erythropoietin therapy to a dog or a cat including administering recombinant...

20060172387 - Chimeric avidin mutants: The present invention relates to new chimeric mutants of avidin protein with improved properties, e.g. thermostability, better stability against proteolysis, better charge properties (for example lower pl) compared to native avidin and avidin-related proteins, AVRs. The chimeric avidin mutants comprise mutants where a region or regions in avidin are substituted...

20060172390 - Consensus phytases: A process for obtaining a consensus protein from a group of amino acid sequences of a defined protein family, proteins and polynucleotides so obtained, and compositions containing such proteins....

20060172375 - D-aminoacylase: A D-aminoacylase produced by a microorganism of genus Defluvibacter; which acts on a N-acetyl-D-amino acid; which has a molecular weight (as determined by electrophoresis) of about 55,000 daltons, and an isoelectric point (as determined by two-dimensional electrophoresis for denatured system) of 5.3; which acts on N-acetyl-D-valine, N-acetyl-D-leucine, and the like,...

20060172384 - Fgf18 production in prokaryotic hosts: The expression vectors and methods using an E. coli expression system for the large scale production of FGF18 are described. The vectors utilize the FGF18 coding sequence with specific changes in nucleotides in order to optimize codons and mRNA secondary structure for translation in E. coli. Using the expression vectors,...

20060172386 - Human fgf-20 gene and gene expression products: This invention relates to human fibroblast growth factor (hFGF-20), and to variants thereof and to polynucleotides encoding FGF-20. The invention also relates to diagnostic and therapeutic agents related to the polynucleotides and proteins, including probes and antibodies, to methods of treating neuronal degenerative disease such as Parkinson's disease and to...

20060172376 - Method and device for the continuous production of biomolecules: The invention relates to a method and device for the continuous production of endogenous or recombinant native polypeptides. The method and device comprise the steps of cultivating recombinant microorganisms in a bioreactor, withdraw a sample of the microbial suspension in the reactor in a continuous manner and subject the same...

20060172388 - Methods for enhancing expression of recombinant proteins: The present invention provides two novel E. coli synthetic promoters, Syn 1 and Syn 2. Syn 1 and Syn 2 are two tight regulated synthetic promoters that control uninduced, leaky expression of proteins that are toxic and interfere with production of recombinant protein from an E. coli expression system....

20060172385 - Methods for producing soluble membrane-spanning proteins: Methods for producing membrane-spanning polypeptides in high yields, with native conformation, and/or in soluble form include solubilizing in non-ionic or zwitterionic detergents, as well as use of promoters and expression vectors for expressing high yields of membrane-spanning polypeptides in bacterial cells. Mutated promoters provide tight control of membrane-spanning polypeptides in...

20060172379 - Methods for transforming and expression screening of filamentous fungal cells with a dna library: The present invention relates to methods for expression screening of filamentous fungal transformants, comprising: (a) isolating single colony transformants of a DNA library introduced into E. coli; (b) preparing DNA from each of the single colony E. coli transformants; (c) introducing a sample of each of the DNA preparations of...

20060172373 - Mutant recombinases: The invention provides hyperactive mutant recombinases and hybrid mutant recombinases, and methods for their identification. Also provided are nucleic acids encoding hyperactive mutant recombinases and hybrid recombinases, as well as vectors and host cells. Host cells include eukaryotic cells capable of expressing said recombinases and carrying out site-specific recombination in...

20060172374 - Phosphorylated ndr kinase: The present invention relates to the phosphorylated form of nuclear serine/threonine protein kinase, designated nuclear, Dbf2-related kinase (Ndr), and provides assays and materials for identifying modulators thereof. The invention relates to the fields of molecular biology, chemistry, pharmacology, and screening technology....

20060172383 - Process for constructing strain having compactin hydroxylation ability: The present invention is directed to methods and compositions for microbial based production of pravastatin. The compositions of the invention include novel strains of microorganisms that are capable of efficiently hydroxylating compactin (ML-236 B) resulting in production of pravastatin. In particular, the microorganisms of the invention are genetically engineered to...

20060172382 - Selection of host cells expressing protein at high levels: The invention provides a DNA molecule comprising a multicistronic transcription unit coding for i) a selectable marker polypeptide functional in a eukaryotic host cell, and for ii) a polypeptide of interest, the polypeptide of interest having a translation initiation sequence separate from that of the selectable marker polypeptide, characterized in...

20060172377 - Site-specific serine recombinases and methods of their use: The present invention provides a method for obtaining site-specific recombination in a eukaryotic cell, the method comprising providing a eukaryotic cell that comprises a first recombination attachment site and a second recombination attachment site; contacting the first and second recombination attachment sites with a prokaryotic recombinase polypeptide, resulting in recombination...

20060172380 - Tab1 protein and dna coding therefore: TAB1 protein having activity which activates factor TAK1 in the TGF-β signaling pathway, and having the amino acid sequence shown in FIG. 1....

20060172381 - Targeted trans-splicing of highly abundant transcripts for in vivo production of recombinant proteins: The present invention provides methods and compositions for generating novel nucleic acid molecules through RNA trans-splicing that target a highly expressed pre-mRNA and contain the coding sequence of a protein or polypeptide of interest. The compositions of the invention include pre-trans-splicing molecules (PTMs) designed to interact with the target precursor...

20060172378 - Variants of protein kinases: The present invention concerns nucleic acid sequences and amino acid sequences of dominant negative variants of kinases, i.e. of sequences which inhibit activity of kinases in a dominant manner. The invention also concerns pharmaceutical compositions and detection methods using these sequences....

20060172392 - Water soluble beta-glucan, glucosamine, and n-acetylglucosamine compositions and methods for making the same: Glucosamine, N-acetylglucosamine and β-glucan compositions suitable for human or animal consumption or use are disclosed. The glucosamine, N-acetylglucosamine and β-glucan compositions are derived from fungal biomass containing chitin. Various methods of producing glucosamine, N-Acetylglucosamine and β-glucan compositions are also disclosed....

20060172393 - Process for producing optically active alpha -methylcysteine derivative: A process for conveniently and industrially producing an optically active α-methylcysteine derivative, which is useful as an intermediate of medicines and the like, from an inexpensive and readily available material is provided. The present invention relates to a process for producing a racemic or optically active α-methylcysteine derivative including a...

20060172394 - Polyhydroxyalkanoate synthesizing microorganism and process of producing polyhydroxyalkanoate using the same: Microorganisms showing higher productivity of unusual-polyhydroxyalkanoate (PHA) than ever by using an inexpensive substrate or under specific conditions are obtained from wild strains and mutant strains. The microorganisms are cultured with such substrates to synthesize an unusual-polyhydroxyalkanoate. The unusual-polyhydroxyalkanoate can be produced with high efficiency at low cost....

20060172395 - Polynucleotides for production of farnesyl dibenzodiazepinones: This invention provides genes and their encoded proteins, involved in the biosynthesis of farnesyl dibenzodiazepinones, including ECO-04601. The invention relates to expression vectors comprising the genes and to host cell transformed with these vectors. The invention further relates to methods of producing farnesyl dibenzodiazepinone compounds using the genes and proteins...

20060172396 - Novel aldolase, and method for producing optically active ihog and monatin: The present invention relates to a method for producing optically active IHOG, which can in turn be used for the production of monatin. The present invention further relates to a method for producing optically active monatin, and aldolase used for these methods. As such, the present invention enables the synthesis...

20060172399 - Acetyl-coa acyltransferase gene disrupted bacterium for producing polyhydroxyalkanoate and method for producing polyhydroxyalkanoate using the same: A method for producing a polyhydroxyalkanoate with improved productivity and composition is provided. Polyhydroxyalkanoate is produced by a bacterium for producing polyhydroxyalkanoate in which a gene encoding acetyl-CoA acyltransferase is disrupted....

20060172400 - Bacterium for producing polyhydroxyalkanoate having polyhydroxyalkanoate depolymerase gene disrupted and method for producing polyhydroxyalkanoate using the same: The objective of the present invention is to provide a bacterium for producing polyhydroxyalkanoate with improved productivity. To achieve above objective, the gene coding for the polyhydroxyalkanoate depolymerase of the bacterium for producing polyhydroxyalkanoate is disrupted to improve the productivity of polyhydroxyalkanoate....

20060172398 - Isogenic strain line of bacterium for producing polyhydroxyalkanoate in which polyhydroxyalkanoate synthase gene is disrupted and method for producing polyhydroxyalkanoate using the same: A host-vector system which is equipped with a substrate supply system enzyme for polyhydroxyalkanoate synthase and which is suitable for evolutionary engineering modification of polyhydroxyalkanoate synthase. An isogenic strain line is produced by disrupting a polyhydroxyalkanoate synthase gene of a bacterium for producing polyhydroxyalkanoate....

20060172397 - Preparation of chiral propargylic alcohol and ester intermediates of himbacine analogs: This application discloses a novel process for the conversion of a series of racemic propargylic alcohols to corresponding (R)-enantiomers. The application also discloses the enantio-selective esterification of a propargylic alcohol from its racemate to prepare an (R)-ester. Enantioselectivity is enhanced by the use of experimentally determined enzymes. The propargylic alcohols...

20060172401 - Method for producing organic acid: An object to be solved by the present invention is to provide a method for producing organic acid with higher fermentation efficiency. The present invention provides a method for producing organic acid from an organic material by allowing bacterial cell or treated products thereof to act on an aqueous reaction...

20060172402 - Production of vanillin in microbial cells: Transgenic microorganisms that produce vanillin when provided with caffeic acid or an esterified or other derivative thereof are disclosed. The organisms are transformed with expressible nucleic acid sequences encoding (1) a 3-0-methyltransferase, preferably from a plant source, which converts caffeic acid to ferulic acid and (2) either a eukaryotic (preferably...

20060172403 - Polypeptides having glucoamylase activity and polynucleotides encoding same: The present invention relates to polypeptides having glucoamylase activity and isolated polynucleotides encoding said polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides. The invention also relates to the composition comprising a glucoamylase...

20060172404 - Methods of screening for compounds that inhibit the biosynthesis of gpi in malaria parasites: The present inventors succeeded in isolating GWT1 (PfGWT1), which is one of the enzymes involved in GPI biosynthesis in the malaria parasite P. falciparum. In addition, the inventors revealed that degenerate mutant DNAs, with a lower AT content than the DNA encoding the PfGWT1 protein, can complement the phenotype of...

20060172405 - Sonication enhanced digestion process: A system and method are provided for sonication-enhanced digestion of cellular matter. The method includes supplying cellular matter to a bioreactor, sonically disrupting the cellular matter, and providing the disrupted cellular matter for microbial digestion in the bioreactor. The system includes a bioreactor for cellular matter, the bioreactor having an...

20060172406 - Method and apparatus for production and refinement of microbial consortia and method of use of microbial consortia as therapeutic agent: A method of producing a rusticle consortia includes the steps of placing one or more samples obtained from a rusticle on a growth substrate, placing the growth substrate with the samples thereon into a tank filled with an aqueous solution, and controlling an environment of the aqueous solution to promote...

20060172407 - Bacterial cell digestion: The invention relates to the use of substances which bind to the bacterial elongation factor EF-Tru for cell digestion or for the lysis of cells. The invention further relates to a cell digestion system and a cell digestion method....

20060172408 - Device for immobilizing chemical and biochemical species and methods of using same: A substrate is provided that facilitates immobilization of nucleic acid molecules, including DNA molecules and RNA molecules. Also provided is a device that includes the substrate, which, for example, can be a chip. In addition, methods of using the substrate are provided, including, for example, methods of sequencing a DNA...

20060172409 - Micro-reactor, dissociation constant measuring apparatus, dissociation constant measuring method, and dissociation constant measuring program: In order to make it possible to predict a dissociation constant at an initial stage of reaction, a concentration sensor for a sample is provided on a downstream side of a mass sensor. This makes it possible to learn concentration of a sample solution at the time when a mass...

20060172410 - Apparatus for use in vermiculture: Apparatus for use in a vermiculture plant, the apparatus including a treatment receptacle having a treatment Apparatus for use in a vermiculture plant which includes a treatment receptacle having a treatment chamber therein and a base upon which material within the treatment chamber is disposed. The receptacle is arranged so...

20060172411 - Composter: The composter provided by the invention is intended primarily for composting household waste, i.e. organic waste such as food waste and the like. To this end the composter comprises a rotatable drum with an inlet at an upstream end thereof and an outlet at a downstream end thereof, whereby, when...

20060172412 - Support device for cells culture: a cell culture device is disclosed having at least one cell-supporting layer of porous material obtained by dehydration of an aqueous gel which is poured directly either into said well bottom or into the bottom of a cradle or insert of a size which is adapted for being insertable into...

20060172413 - Transgenic mice containing melanocyte stimulating hormone receptor gene disruptions: The present invention relates to transgenic animals, as well as compositions and methods relating to the characterization of gene function. Specifically, the present invention provides transgenic mice comprising disruptions in melanocyte stimulating hormone receptor genes. Such transgenic mice are useful as models for disease and for identifying agents that modulate...

20060172414 - Feeder layer and serum independent embryonic stem cells: Undifferentiated primordial stem cells are manipulated to permit their long term growth in defined media lacking serum and feeder layer cells by shifting the apoptotic balance of the cells, through increasing the activity of Bcl-2 family anti-apoptotic proteins or decreasing the activity of Bcl-2 family pro-apoptotic proteins. In some embodiments...

20060172415 - Oligodendrocyte precursor cells and method of obtaining and culturing the same: The invention describes a self-renewing, phenotypically homogeneous population of oligodendrocyte precursor cells having a synchronized developmental stage and methods of obtaining a self-renewing phenotypically homogeneous population of oligodendrocyte precursor cells. Other methods include methods of maintaining and storing a homogeneous population of oligodendrocyte precursor cells for a prolonged period of...

20060172416 - Cell culture and method for screening for a compound useful in the treatment or prevention of hepatic cirrhosis: The present invention provides a cell-culturing model and a method for screening compounds which can be applied in treating or preventing hepatic cirrhosis. The cell culturing model comprises a population of hepatocytes and hepatic stellate cells (HSCs) derived from co-culturing, and at least one population of the cells comprises a...

20060172417 - Cell cultivation and breeding method: The invention relates to a cell cultivation method, which may comprise the steps of preparing a carbon-based substrate with a layered structure, composed of at least two porous material layers, substantially superimposed and joined to each other, a gap which can be flowed through being formed between said layers, or...

20060172420 - Gene trap system: The present invention provides a gene-trap system, which can trap any gene including transiently expressed genes. A gene can be trapped by a trap vector, which employs Cre recombinase from bacteriophage P1, and the expression of the trapped gene is converted to constitutive expression of another reporter gene....

20060172419 - Highly efficient gene targeting vectors and methods for gene targeting method forward epithelial cell line: In the process of generating ZO-1 gene knockout mice, the present inventors developed gene targeting vectors that allow homologous recombination to occur at a high probability (90% or more). In addition, the present inventors used the targeting vectors for studying optimal electroporation conditions in epithelial cells, and as a result,...

20060172418 - Hypercoiling polymers and their use in cellular delivery: This invention pertains to certain hypercoiling polymers, and their use for the delivery of a payload into a living cell, e.g., into the nucleus of a living cell, which polymer incorporates, or is otherwise associated with, said payload. The payload may be, for example, a therapeutic payload, such as a...

20060172421 - Novel cosmid vector: A novel cosmid vector and the like effectively used in generating a recombinant adenoviral vector are provided. More specifically, there are provided a cosmid vector characterized by: (1) containing an adenoviral genome having adenoviral inverted terminal repeat sequences each having a complete nucleotide sequence, (2) having a deletion in an...

20060172422 - Computerized electroporation: Techniques for computerized electroporation. An electroporation apparatus may be controlled according to one of a plurality of previously-saved, user-defined processing protocols. A processing log associated with a processing protocol may be generated, and the processing log may include patient or sample specific information. The processing log or a summary of...

20060172424 - Methods for enhancing expression of recombinant proteins: The present invention provides two novel E. coli synthetic promoters, Syn 1 and Syn 2. Syn 1 and Syn 2 are two tight regulated synthetic promoters that control uninduced, leaky expression of proteins that are toxic and interfere with production of recombinant protein from an E. coli expression system....

20060172423 - New expression system from rhodococcus: The present invention provides an isolated polynucleotide comprising the kstD promoter from Rhodococcus erythrypolis. The polynucleotide can very advantageously be used as a controllable transcription activator. Said controlling function can be provided by providing said isolated polynucleotide with a nucleotide sequence encoding a transcription regulator of said promoter. In the...