Chemistry: molecular biology and microbiology inventions 07/06

20060166182 - Tissue and organ preservation, protection and resuscitation: The present invention provides compositions and methods for protecting tissues and organs from damage during transplantation or from acute ischemia due to, e.g., injury or surgery. The compositions protect the tissue or organ from acidosis, oxidative damage, ischemia and repurfusion injury while the organ is isolated from the normal circulation...

20060166184 - Apparatus for cell reaction examination and method of cell reaction examination: Disclosed herein are an inspection apparatus for cell reaction by a device for liquid processor, which can set a fluid running route to a cell reaction site required according to the kind of an inspection of a cell reaction to be conducted with a great degree of freedom, and an...

20060166183 - Preparation of coatings through plasma polymerization: The invention provides a method to prepare at least part of at least one surface of a substrate comprising; depositing on said surface at least one plasma monomer wherein during deposition of said monomer, means are provided which move the monomer source across a surface to be treated to manufacture...

20060166185 - System for and method of patch clamp analysis: A system and method for automated patch clamp analysis of biological tissue such as cellular membrane. A biological membrane is applied to a porous semiconductor substrate, and laser energy is imparted thereon. Voltage clamped electrodes detect current across the membrane to indicate ion channel activity....

20060166186 - Akap84 and its use for visualization of biological structures: A polynucleotide encoding a chimeric polypeptide is provided. The chimeric polypeptide includes (a) a first polypeptide region being capable of specifically binding at least one detectable molecule; and (b) a second polypeptide region being capable of specifically binding a biological component or macromolecule or targeting a cellular compartment. Methods utilizing...

20060166193 - Binding protein: HCV assays are described. The assays utilize a 24kd protein capable of binding the E2 envelope protein of hepatitis C virus (HCV), or functionally equivalent variants or fragments of the 24kd protein....

20060166192 - Detection of protease-resistant prion protein following a spontaneous transformation reaction: The present invention relates to a method for detecting infectious or pathogenic prion protein with an improved degree of sensitivity. For this, protease-resistant prion protein PrPSc is formed de novo by means of a spontaneous transformation reaction, with nonpathogenic prion protein PrPc in the sample interacting with protease-sensitive low molecular...

20060166187 - Hbv precore protein capable of forming particles: There is provided a HBV precore protein having an ability of forming particles, and a means for determining it. A novel HBV precore protein that forms the virus (like) particles of HBV was identified. The present invention provides this novel HBV precore protein. Furthermore, there are provided core-like particles and...

20060166191 - Inhibition of ap-3/gag interactions in the treatment of human immunodeficiency virus infections: The present invention provides for methods of identifying potential inhibitors of HIV infection and replication. More specifically, the invention identifies complexes between the δ subunit of AP-3 and Gag, which facilitate HIV assembly. Screening for inhibitors of this interaction will identify lead compounds for the treatment of HIV and AIDS....

20060166190 - Magnetism based nucleic acid amplification: This invention relates generally to the field of nucleic acid amplification. In particular, the invention provides processes and kits for amplifying a nucleic acid of a target cell or virus using, using inter alia, binding between a target cell, cellular organelle or virus with a magnetic microbead....

20060166189 - Methods for detecting invasion of a cell: The present invention provides a rapid method to determine invasion of a cell by an invasion....

20060166188 - Methods of examining swine genetic resistance to rna virus-origin disease: The inventors investigated the impact of an 11-bp deletion in the swine Mx1 gene on the ability to suppress propagation of influenza viruses belonging to the myxovirus family, and revealed that the deletion led to a complete loss of the ability to suppress viral propagation. Through the detection of the...

20060166236 - Allosteric enzyme coupled immunoassay (aecia): The present invention is directed to methods and compositions for an allosteric enzyme coupled assay, and preferably to an allosteric enzyme coupled immunoassay (AECIA). The assay uses an allosteric enzyme to generate a readout signal. The assay is based on the competition between an analyte in a sample and an...

20060166259 - Apm1 biallelic markers and uses thereof: The invention provides novel APM1 genomic sequences, polypeptides, antibodies, and polynucleotides including biallelic markers derived from the APM1 locus. Primers hybridizing to regions flanking these biallelic markers are also provided. This invention also provides polynucleotides and methods suitable for genotyping a nucleic acid containing sample for one or more biallelic...

20060166234 - Apparatus and system having dry control gene silencing compositions: An RTF testing plate can include at least a first control well including a substantially dry first control composition having at least a first control siRNA. The first control siRNA is capable of providing a first indication of the gene silencing efficacy. Additionally, the first control composition can be configured...

20060166215 - Assay and process for labeling and detection of micro rna and small interfering rna sequences: A process for protecting a short RNA fragment includes labeling a short RNA fragment with a detectable platinum compound forming a labeled small RNA fragment. Resulting labeled short RNA fragment is exposed to a capture oligonucleotide. The capture oligonucleotide includes at least two replicates of a nucleotide sequence complimentary to...

20060166224 - Associations using genotypes and phenotypes: The present invention discloses methods for combining data on genetic variations and phenotypes of individuals to predict a phenotype-of-interest. The present invention also discloses kits that can be used to determine if an individual has or does not have a phenotype-of-interest. The kit can include at least one diagnostic tool...

20060166216 - Biochemical reaction system, biochemical reaction substrate, process for producing hybridization substrate and hybridization method: A bioassay substrate (1) is flat and has a disc-shaped main side like an optical disc such as CD. The substrate (1) is rotatable about a central hole (2) formed therein. The substrate (1) has formed on the surface (1a) thereof a plurality of wells (8) where a probe-use DNA...

20060166212 - Breast specific protein expressed in cancer and methods of use thereof: Cancer-linked gene sequences, and derived amino acid sequences, are disclosed along with processes for assaying potential antitumor agents based on their modulation of the expression of these cancer-linked genes. Also disclosed are antibodies that react with the disclosed polypeptides and methods of using the antibodies to treat cancerous conditions, such...

20060166197 - Building block capable of transferring a functional entity: A building block having the dual capabilities of transferring the genetic information e.g. by recognising an encoding element and transferring a functional entity to a recipient reactive group is disclosed. The building block can be designed with an adjustable transferability taking into account the components of the building block. The...

20060166220 - Chimeric tumor suppressor gene and protein: The present invention relates to chimeric molecules comprising portions of rat PEG-3 (“rPEG-3”) and human GADD34 (“hGADD34”) having apoptotic activity. It is based, at least in part, on the discovery that a chimeric protein comprising amino acids 1-347 of rat PEG-3 fused with residues 418-674 of human GADD34 exhibited anti-proliferative...

20060166200 - Compositions of solution for sequencing reaction clean-up: Wash solution and method for purifying sequencing reaction product. The wash solution comprises an effective amount of guanidine in a low ionic solution to reduce or eliminate the presence of dye terminators in a sequencing reaction product In its method aspects, the present invention comprises the addition of the wash...

20060166257 - Converting diploidy to haploidy for genetic diagnosis: Detection of mutations associated with hereditary diseases is complicated by the diploid nature of mammalian cells. Mutations present in one allele are often masked by the wild-type sequence of the other allele. Individual alleles can be isolated from every chromosome within somatic cell hybrids generated from a single fusion. Nucleic...

20060166205 - Detecting the risk of cardiovascular disease by detecting mutations in genes, including genes encoding a2b-adrenoceptor and apoliporotein b: The present invention provides a method of identifying subject's susceptibility to cardiovascular diseases or risk of developing myocardial infarction (MI) or cerebrovascular stroke by detecting gene polymorphisms and other gene mutations from a biological sample of the subject and optionally obtaining information concerning the family and medical history, blood, serum,...

20060166246 - Detection of fungal pathogens using the polymerase chain reaction: The present invention relates to the use of primers in polymerase chain reaction assays for the detection of fungal pathogens Colletotrichum acutatum, Ateniaria spp., and Cladosporium carpophilum. Specific primers are identified as being useful for the identification of fungal isolates using PCR based techniques. Also described are novel extraction buffer...

20060166194 - Diagnosis and treatment of cancer: A method of diagnosing cancer in a human patient comprising the steps of (i) obtaining a sample containing nucleic acid and/or protein from the patient; and (ii) determining whether the sample contains a level of hNeNa voltage-gated Na+ channel nucleic acid or protein associated with cancer. A method of treating...

20060166226 - Dna amplification device: At the same time, at least the thickness Ld of regions Xc . . . situated under the cells C . . . in the lower substrate 7 is selected to be 1.0 [mm] or thinner, and, a thermo-module(s) comes into contact with the lower surface of the base 5....

20060166244 - Dna markers for increased milk production in cattle: The invention provides methods for identifying a genetic polymorphism associated with altered milk production traits in dairy cattle. Genetic marker-assisted selection methods provided by the invention allow avoidance of potentially costly phenotypic testing and inaccuracies associated with traditional breeding schemes and improvement of dairy cattle herds....

20060166223 - Dna purification and analysis on nanoengineered surfaces: A microfluidic device for isolating genomic DNA from human leukocytes, simultaneously capturing and measuring the DNA for simplifying genetic analysis. The device contains a fluid inlet port, a fluid outlet port, and a DNA binding channel in contact with the fluid inlet port wherein at least a portion of at...

20060166264 - Effect of comt genotype on frontal lobe function: The invention provides a method of detecting impaired prefrontal cognitive function in an individual by determining the individual's COMT genotype, and associating a high activity Val allele with impaired prefrontal cognitive function (and a low activity Met allele with enhanced prefrontal cognitive function)....

20060166248 - Fluorescent n2, n3-etheno-purine (2'deoxy) riboside derivatives and uses thereof: The invention relates to fluorescent N2,N3-etheno-purine (2′-deoxy) riboside derivatives and fluorescent oligonucleotide probes comprising one or more moieties thereof, their preparation and uses thereof for staining DNA/RNA and for detection and quantitation of genetic material....

20060166217 - Genetic marker for coronary artery disease: The present invention describes a Coronary artery disease (CAD) susceptibility gene and the use of this gene in the development of novel therapies to treat CAD and in the diagnosis of susceptibility to CAD....

20060166239 - Genetic variants predicting warfarin sensitivity: We discovered that a polymorphism in the promoter of the VKORC1 gene is associated with warfarin sensitivity. This polymorphism can explain both the inter-individual and inter-ethnic differences in warfarin dose requirements. Furthermore, the polymorphism is also associated with promoter activity. Thus, the promoter sequence or activity of the VKORC1 gene...

20060166209 - Growth hormone variations in humans and its uses: The present invention relates to a naturally-occurring growth hormone mutation; to a method for detecting it and its use in screening patients for growth hormone dysfunction or for producing variant proteins suitable for treating such irregularities....

20060166247 - High throughput assay for cancer cell growth inhibition: A high-throughput, anchorage-independent assay is described, which screens compounds for inhibition of cancer cell growth. The assay utilizes a three-dimensional matrix or semi-solid media transfected with the subject compound, and enables live colony growth determination and imaging....

20060166207 - Hsan ii related gene and expression products and uses thereof: Methods of utilizing a gene related to pain perception, herein dubbed HSN2, or its encoded protein, dubbed herein “sensorin,” for the screening and identification of agents for the treatment of pain, neuropathy and related disorders, especially small organic compounds, as well as methods of using these compounds to treat or...

20060166256 - Il-1 receptor accessory protein: DNA encoding IL-1R AcP splice variant polypeptides, the polypeptides and methods for using the encoded polypeptides are disclosed....

20060166208 - Induction of the mitochondrial permeability transition: The present invention relates to process for identifying a compound which induces the mitochondrial permeability transition (MPT) in proliferating cells, wherein said process comprises contacting a cell or cell extract with a compound, determining whether compound binds to adenine nucleotide translocator (ANT), and determining whether the compound selectively induces the...

20060166232 - Internal control nucleic acid molecule for nucleic acid amplification systems: The invention provides an internal control nucleic acid molecule including at least one forward primer binding site, at least one reverse primer binding site, and at least one amplifiable region, wherein the forward primer binding site, the reverse primer binding site, and the amplifiable region are all randomly generated. The...

20060166250 - Isothermal dna amplification: The present invention provides for amplification of one or more polynucleotides by multi-staged linear amplifications using one or more RNA polymerases. At each stage RNA transcripts are accumulated at a linear rate, so that multiple stages provide for faster than linear transcript accumulation. In one aspect, the invention provides for...

20060166202 - Lipid phosphate phosphatases and uses thereof for treating neuronal diseases: Lipid phosphate phosphatase proteins, genes coding for them, vectors and cells comprising them, antibodies directed against them, methods of identifying compounds binding to them and functional interactors as well as to the use of proteins, genes, vectors, cells, interacting compounds and functional interactors for treating neuronal diseases and/or injuries....

20060166214 - Marker for detecting mesenchymal stem cell and method of distinguishing mesenchymal stem cell using the marker: The present invention provides a marker for detecting, separating/distinguishing a mesenchymal stem cell and a method for detecting, separating/distinguishing a mesenchymal stem cell by using the marker. A gene shown in the sequence listing is expressed specifically in a mesenchymal stem cell. The present invention comprises use of the gene...

20060166228 - Markers of alterations in the y chromosome and uses therefor: Novel sequence tagged sites (STSs), probes and primers useful, e.g., for detecting the presence or absence of an STS in a sample, and methods of using these STSs, probes and primers, e.g., in methods of detecting alterations in the Y chromosome are disclosed. These compositions are also useful in methods...

20060166242 - Markers of pre-term labor: The invention relates to novel markers of pre-term labor, methods for assessing the status of pre-term labor using the markers, and methods for the diagnosis and therapy of pre-term labor....

20060166233 - Method and apparatus for processing polynucleotide-containing samples: Methods and systems for processing polynucleotides (e.g., DNA) are disclosed. A processing region includes one or more surfaces (e.g., particle surfaces) modified with ligands that retain polynucleotides under a first set of conditions (e.g., temperature and pH) and release the polynucleotides under a second set of conditions (e.g., higher temperature...

20060166229 - Method enabling use of extracellular rna extracted from plasma or serum to detect, monitor or evaluate cancer: This invention relates to the use of tumor-derived or associated extracellular ribonucleic acid (RNA) found circulating in the plasma or serum fraction of blood for the detection, monitoring, or evaluation of cancer or premalignant conditions. Extracellular RNA may circulate as non-bound RNA, protein-bound RNA, lipid-RNA complexes, lipoprotein (proteolipid)—RNA complexes, protein-RNA...

20060166195 - Method for detecting and identifying the presence of biological materials derived from fish and oligonucleotides therefor: A subject of the present invention is a method for detecting the presence of biological materials originating from gadiformes chosen from the group constituted by the gadidae, the merluccidae, the macrouridae and/or the moridae, in a sample of organic material, characterized in that the presence of mitochondrial DNA originating from...

20060166198 - Method for in vitro molecular evolution of protein function: A method for in vitro molecular evolution of protein function The invention provides a method for generating a polynucleotide sequence or population of sequences from parent single-stranded polynucleotide sequences encoding one or more protein motifs, comprising the steps of (a) providing a first population of single-stranded polynucleotide molecules and a...

20060166201 - Method for the detection of nucleic acid sequences by means of crackable probe molecules: The present invention describes a method for the detection of nucleic acid sequences, which is characterized in that the following steps are conducted: a) at least one nucleic acid is bound to a solid phase; b) probe molecules are hybridized to the nucleic acids in a sequence-specific manner, whereby the...

20060166237 - Method of detecting reaction of dna and dna-binding protein: In the case of detecting whether or not a binding site of a transcription factor TFIID and a transcription factor TFIIB is present in a labeled DNA, the presence of a binding site can be detected precisely by adding all of a transcription factor TFIID, a transcription factor TFIIB and...

20060166240 - Method of optimizing amplification in pcr: The present invention discloses a method for optimizing PCR amplification by adding elementary substance material into PCR system, wherein the elementary substance material is selected from a group consisting of element titanium, element nickel, element bismuth, element stibium, element selenium, element chromium, and a mixture of the group. This new...

20060166210 - Methodology of estimating the conformation of a protein by proteolysis: The invention relates to a method for determining the effect of a polymorphism or mutation on the structural properties of a protein wherein the method relies on the structural properties of the protein and so its cleavage during proteolysis....

20060166206 - Methods and compositions for analysis of regulatory sequences: Methods for constructing arrays of regulatory sequences, and the arrays so obtained, are provided. Regulatory sequences for use on the arrays are isolated based on their accessibility in cellular chromatin. A number of methods for using the arrays are disclosed, including regulatory DNA profiling, epigenome profiling, toxicological profiling and identification...

20060166225 - Methods and compositions for polypeptide engineering: Methods are provided for the evolution of proteins of industrial and pharmaceutical interest, including methods for effecting recombination and selection. Compositions produced by these methods are also disclosed....

20060166249 - Methods for separating short single-stranded nucleic acid from long single-and double-stranded nucleic acid, and associated biomolecular assays: Methods and kits are provided for detecting the presence or absence of target nucleic acid sequences in a sample. The methods and kits involve the use of negatively charged nanoparticles and the electrostatic interactions between the metal nanoparticles and nucleic acid molecules. The methods rely upon the differential interaction of...

20060166221 - Methods of diagnosing essential thrombocythemia: This invention relates to diagnosis of essential thrombocythemia. More specifically, this invention provides methods of diagnosing essential thrombocythemia by detecting a decrease in gene expression or protein levels of type 3 17β-hyrdroxysteroid dehydrogenase, and an increase in gene expression or protein levels of type 12 17β-hyrdroxysteroid dehydrogenases, in a test...

20060166255 - Methods of using 48149, a human aminopeptidase family member: Isolated nucleic acids molecules, designated 48149 nucleic acid molecules, which encode human Aminopeptidase N, are disclosed. The invention provides methods of modulating 48149 activity, which is associated with the formation of atherosclerotic lesions in blood vessels. The invention further provides methods of treating, preventing and diagnosing cardiovascular disorders such as...

20060166199 - Methods to assess quality of microarrays: The present invention relates to methods and compositions for assessing the quality of microarrays. In particular, the invention relates to the use of quality control probes that are synthesized on the microarray monomer by monomer in a step-by-step synthesis. By assessing the degree of signal from the quality control probes...

20060166243 - Methods to increase nucleotide signals by raman scattering: The methods and apparatus disclosed herein concern nucleic acid sequencing by enhanced Raman spectroscopy. In certain embodiments of the invention, nucleotides are covalently attached to Raman labels before incorporation into a nucleic acid. In other embodiments, unlabeled nucleic acids are used. Exonuclease treatment of the nucleic acid results in the...

20060166235 - Methods, compositions, and kits for forming labeled polynucleotides: The present teachings provide novel methods, compositions, and kits for forming multi-labeled polynucleotides. In some embodiments of the present teachings, multiplexed amplification reactions are performed with a plurality of primer pairs, wherein one primer in a given primer pair comprises a distinct label. Additional labeling of the resulting amplicons can...

20060166231 - Molecular indicators of breast cancer prognosis and prediction of treatment response: The present invention relates to quantitative molecular indicators that can guide clinical decisions in breast cancer, such as estrogen receptor (ESR1)-positive, lymph node-negative breast cancer. In particular, the invention concerns certain genes, the varied expression of which indicates the likelihood of recurrence of surgically resected breast cancer in patients who...

20060166203 - New sequencing method for sequencing rna molecules: The present invention provides a method for determination of the identity of at least one nucleotide in a RNA-molecule comprising the steps of: (i) providing the RNA-molecule, an oligonucleotide primer binding to a predetermined position of the RNA molecule, a reverse transcriptase, deoxynucleotides and other necessary reagents, in a reaction...

20060166213 - Novel compositions and methods in cancer: The present invention relates to novel sequences for use in detection, diagnosis and treatment of cancers. The invention provides cancer-associated (CA) polynucleotide sequences whose expression is associated with cancer. The present invention provides CA polypeptides associated with cancer and provides diagnostic compositions and methods for the detection of cancer. The...

20060166252 - Novel methods of constructing libraries of genetic packages that collectively display the members of a diverse family of peptides, polypeptides or proteins: Methods useful in constructing libraries that collectively display members of diverse families of peptides, polypeptides or proteins and the libraries produced using those methods. Methods of screening those libraries and the peptides, polypeptides or proteins identified by such screens....

20060166219 - Ntrk1 genetic markers associated with progression of alzheimer's disease: Haplotypes in the NTRK1 gene associated with progression of Alzheimer's Disease are disclosed. Compositions and methods for detecting these NTRK1 haplotypes are disclosed....

20060166222 - Nucleic acid enzyme ligation sensor: The present invention provides a calorimetric light-up sensor for determining the presence and optionally the concentration of an analyte in a sample. Methods of utilizing the sensor and kits that include the sensor also are provided. The sensor utilizes nucleic acid enzymes that ligate substrate fragments to form aggregates from...

20060166241 - Nucleic acid isolation unit and method using intercalator: Provided are nucleic acid isolation unit and method. The method includes immobilizing an aromatic compound-containing nucleic acid intercalator on a solid support; contacting a first buffer solution containing a nucleic acid sample to be purified to the intercalator immobilized on the solid support to bind the intercalator with nucleic acids...

20060166204 - Parkinson's disease susceptibility haptotype as a tool for genetic screening: Specific PON1 and ACHE alleles segregate in linkage, forming an haplotype which directly correlates with higher susceptibility to develop Parkinson's Disease (PD). This PD-susceptibility haplotype is herein presented as a tool for predicting the risk of developing Parkinson's Disease and its severity, both for an individual and for the population...

20060166261 - Pcr and hybridization methods utilizing electrostatic transportation and devices therefor: A biological sample (droplets containing DNA and primer) (9) is prepared in a chemically inert liquid layer (8), is electrostatically transported by the action of an electrostatic transportation plate. (1) with electrostatic transportation electrodes (2) and is heated at a predetermined position of the electrostatic transportation plate (1), thus carrying...

20060166262 - Pcr and hybridization methods utilizing electrostatic transportation and devices therefor: A biological sample (droplets containing DNA and primer) (9) is prepared in a chemically inert liquid layer (8), is electrostatically transported by the action of an electrostatic transportation plate (1) with electrostatic transportation electrodes (2) and is heated at a predetermined position of the electrostatic transportation plate (1), thus carrying...

20060166230 - Predicting response to chemotherapy using gene expression markers: The present invention provides gene expression information useful for predicting whether cancer patients are likely to have a beneficial response to treatment response with chemotherapy....

20060166258 - Preservation of rna in a biological sample: To preserve RNA in a biological sample for analysis, the sample is incubated with an RNA preservative capable of precipitating RNA in an aqueous solution, such as a triphenylmethane dye (e.g., methyl green, crystal violet, pararosaniline, or tris-(4-aminophenyl)methane), cresyl violet, or cobalt ions. RNA preservation may be used in an...

20060166238 - Probes, libraries and kits for analysis of mixtures of nucleic acids and methods for constructing the same: The invention relates to nucleic acid probes, nucleic acid probe libraries, and kits for detecting, classifying, or quantifying components in a complex mixture of nucleic acids, such as a transcriptome, and methods of using the same. The invention also relates to methods of identifying nucleic acid probes useful in the...

20060166218 - Protective coating for array material deposition: An array is formed with a protective cover on a substrate. The protective cover is patterned to produce an array of openings to the substrate. Desired material is deposited on the substrate through the openings. The protective cover may then be removed. In one embodiment, the protective cover is a...

20060166227 - Protein expression profiling: Disclosed are compositions and methods for detecting small quantities of analytes such as proteins and peptides. The method involves associating a primer with an analyte and subsequently using the primer to mediate rolling circle replication of a circular DNA molecule. Amplification of the DNA circle is dependent on the presence...

20060166254 - Protocol for optimizing purification of enzymatically active human telomerase: This invention provides purified telomerase and methods of purifying it. The methods involve the use of several sequential steps, including the use of matrices that bind molecules bearing negative charges, matrices that bind molecules bearing positive charges, intermediate-selectivity matrices, methods that separate molecules based on their size, shape, or buoyant...

20060166263 - Randomized libraries of zinc finger proteins: The present invention relates to methods of using libraries of randomized zinc finger proteins to identify genes associated with selected phenotypes....

20060166196 - Reporter gene assay method: The invention provides a reporter gene assay method by which the sex hormone-like activity inherent in a test substance can be accurately assayed excluding the effects caused by a decrease in cell activity (protein expressing capacity). The assay method is a reporter gene assay method using luciferase-expressing cells which comprises...

20060166245 - Rolling circle amplification of micro-rna samples: The compositions of the present invention find use in amplifying RNA obtained from subjects, particularly very small RNA samples. The methods allow conversion of RNA into circularized cDNA suitable for amplification by rolling circle replication. The amplified cDNA is then transcribed into RNA resulting in amplified RNA....

20060166211 - Ss 1,3-n-acetyl-d-galactosamine transferase protein, nucleic acid encoding the same and method of examining canceration using the same: The N-acetyl-D-galactosamine transferase protein of the present invention is characterized by transferring N-acetyl-D-galactosamine to N-acetyl-D-glucosamine with β1,3 linkage, and it preferably has the amino acid sequence shown in SEQ ID NO: 2 or 4. The canceration assay according to the present invention uses a nucleic acid for measurement which hybridizes...

20060166265 - Survivin-binding proteins, encoding nucleic acids, and methods of use: In accordance with the present invention, there are provided novel Survivin-binding-proteins (SBPs). Nucleic acid sequences encoding such proteins and assays employing same are also disclosed. The invention SBPs can be employed in a variety of ways, for example, for the production of anti-SBP antibodies thereto, in therapeutic compositions and in...

20060166253 - Tumor tissue microarrays for rapid molecular profiling: An array-based technology facilitates rapid correlated gene copy number and expression profiling of a very large number of human tumors. Hundreds of cylindrical tissue biopsies (diameter 0.6 mm) from morphologically representative regions of individual tumors can be arrayed in a single paraffin block. Consecutive sections from such arrays provide targets...

20060166251 - Use of sfrps as markers of bmp activity: The disclosure provides assay systems for evaluating the presence of bone morphogenetic protein (BMP) activity in a cell by evaluating the gene expression of secreted Frizzled Related Protein 2 and 3 (sFRP2 and sFRP3). The sFRP2 and sFRP3 gene expression may be detected at the RNA or protein levels. The...

20060166260 - Visualization of avian eggs: The invention includes methods of visualizing and manipulating avian eggs employing TPLSM....

20060166275 - Amyloid beta protein (globular assembly and uses thereof): The invention described in this disclosure involves a new composition of matter, amyloid beta-derived dementing ligands (ADDL's). ADDLs consist of amyloid β peptide assembled into soluble globular non-fibrillar oligomeric structures that are capable of activating specific cellular processes. The invention further encompasses methods for assaying the formation, presence, receptor protein...

20060166270 - Detection of demyelination: The present invention relates to a method for the detection of demyelinization in a mammal, comprising providing an NMR spectrum of metabolites in a body fluid of an individual of said mammal in which demyelinization is suspected and comparing said NMR spectrum with a difference profile, comprising a plurality of...

20060166268 - Diagnosis of glaucoma by complex autoantibody repertoires in body fluids: The invention relates to a method for the diagnosis of glaucoma based on the composition of autoantibodies against ocular antigens in body fluids of individuals. The method is characterized in that in a first step, autoantibodies against retinal and/or optic nerve head antigens are detected and measured in body fluids...

20060166273 - Enhanced methods for crystallographic structure determination employing hydrogen exchange analysis: The present invention provides methods for crystallographic structure determination employing hydrogen exchange analysis. Hydrogen exchange analysis is used to identify unstructured regions of a protein, and then hydrogen exchange analysis repeated after the protein is admixed with candidate agents and/or conditions that may induce structure in said identified unstructured regions....

20060166274 - G protein coupled receptor (gpcr) agonists and antagonists and methods of activating and inhibiting gpcr using the same: The invention relates generally to G protein coupled receptors and in particular to agonists and antagonists of G protein receptors and methods of using the same....

20060166280 - Labor biomarkers, methods comprising same, and methods targeting same: The present invention provides methods of predicting or detecting labor in a female subject and methods of testing a compound for an ability to delay the onset of labor. The present invention also provides methods of testing a labor marker useful in the diagnostic methods, isolated peptides identified in the...

20060166267 - Method for extracting proteins: The present invention relates to a method and a kit for the sequential extraction of proteins for the production of partial proteomes from a complete proteome. In particular, the method according to the invention, besides other partial proteomes, gives a partial proteome of proteins from the cell nucleus interior separated...

20060166278 - Method for synthesizing sugar chain(s): An object of the present invention is to provide a method for efficiently chemically synthesizing biomolecules including a nucleotide (nucleic acid), a peptide (protein), or a sugar chain, as representative examples. The present invention provides a method of solid-phase synthesis of sugar chain(s) for synthesizing multiple types of sugar chains...

20060166272 - Method of adjudicating on acute aortic dissection and reagent for adjudication: A method of evaluating acute aortic dissection, which comprises measuring a D-dimer concentration in blood separated from a human, and determining whether or not acute aortic dissection (Stanford type A or Stanford type B) has developed, on the, basis of the measured concentration, a reagent for evaluating performing the evaluation...

20060166276 - Method of diagnosis and agents useful for same: The present invention relates generally to a method of diagnosing, predicting or monitoring the development or progress of heart failure in a mammal and, more particularly, to a method of diagnosing, predicting or monitoring the development or progress of congestive heart failure in a mammal. The present invention contemplates a...

20060166269 - Method of measuring binding site on plasma protein of plasma protein-binding drug and method of measuring plasma protein mutation: The present invention relates to a method for readily determining the binding site on plasma protein and binding displacement effect of a drug having binding affinity to plasma protein, and application of the method to clinical diagnosis. That is to say, the present invention provides a method for determining the...

20060166271 - Non-radioactive schilling test: The present invention relates to a new method named the COBASORB test, which can be used for testing the cause of cobalamin malabsorption in humans. The COBASORB test contains three separate tests (first, second and third test) that can be performed separately, sequentially or in random order and number. The...

20060166277 - Nucleic acids and polypeptides useful for diagnosing and treating complications of pregnancy: Disclosed herein are methods for diagnosing or treating pregnancy related hypertensive disorders that include the use of a polypeptide or a nucleic acid encoding a polypeptide selected from the following: follistatin related protein, interleukin 8, inhibin A, VEGF-C, angiogenin, beta fertilin, hypothetical protein, leukocyte associated Ig-like receptor secreted protein, erythroid...

20060166266 - Screening method for regulatory enzymes employing tribrid (tri-hybrid) system: A method for screening regulatory enzymes is described. The method involves the use of a tribrid cell engineered to express a DNA library....

20060166279 - Sea-trosy and related methods: A method for preferentially observing an exposed position (1c) of a macromolecule. A sample is obtained having a macromolecule (1a) with a first proton (1) and a second molecule (2a) with a second proton (2); then applying a magnetic field (4) to the sample and irradiating the sample with a...

20060166283 - Means for detecting neurodegenerative processes and applications of same: The invention relates to novel markers of the neurodegenerative process, comprising the α chain of the modified ATP synthase. The invention also relates to methods of detection, diagnosis, antibodies, kits and applications of same....

20060166282 - Methods and compositions for screening cell binding molecules: Ligands, such as antibodies, are screened for binding to cell surface moieties such as receptors. Multiple cell types and/or binding ligands can be screened simultaneously in multiplexed assays. The screening assays employ membrane-anchored electrophoretic probes for detection of binding of the ligand to the cell surface....

20060166281 - Potent, selective and non toxic c-kit inhibitors: The present invention relates to a screening method allowing the identification and selection of compounds targeting the transphosphorylase (also called phosphotransferase) domain of c-kit, more particularly compounds selected to be potent inhibitors of constitutively activated c-kit, while being unable to inhibit other activation pathways as for example the pathways leading...

20060166294 - Apoptosis marker antibodies and methods of use: Disclosed are antibodies that specifically recognize the new amino terminus of a protein cleaved by a protease during apoptosis. Methods of using and making the antibodies are also provided. The antibodies are particularly useful in methods of detecting apoptosis and testing candidate compounds for enhancing or inhibiting apoptosis....

20060166288 - Assay for the screening of compounds acting through erbb-2: The invention provides a cellular proliferation assay for a compound acting through erbB-2 which comprises: i) a cell comprising erbB-2 and erbB-3 and said cell is responsive to ligand stimulated cell proliferation under conditions suitable for cell proliferation; ii) a first ligand which is a ligand for erbB-3 capable of...

20060166291 - Kid31 and antibodies that bind thereto: The invention provides the identification and characterization of disease and cancer-associated antigen, KID31. The invention also provides a family of monoclonal antibodies that bind to antigen KID31, methods of diagnosing and treating various human cancers and diseases that express KID31....

20060166289 - Method for analyzing tumor agressivity comprising measurement of polymerized actin: The invention concerns a method for analyzing tumor agressivity of cancer cells comprising measurement of the amount of stationary polymerized actin in a lysate of said cells. Advantageously, the measurement of the amount of stationary actin is carried out by fluorescence static polarization....

20060166287 - Method for identifying compounds for suppressing metastasizing tumor cells: The present invention relates to a method for identifying compounds which suppress and/or delay the development of invasive or metastasizing tumor cells of the colon, of the pancreas, of the ovaries or of the thyroid....

20060166292 - Methods of diagnosing and treating prostate cancer: The invention provides for novel diagnostic methods for detecting prostate cancer. The invention also provides methods for determining the effectiveness of prostate cancer treatment. These methods take advantage of the finding that a cellular protein termed Beta Protein 1 (BP1) is expressed at elevated levels in prostate cancer tissue and...

20060166290 - Primary central nervous system tumor specific behab isoforms: The present invention comprises compositions and methods related to a glycosylation-variant BEHAB polypeptide, a poly-sialyated BEHAB polypeptide, and methods of use thereof....

20060166293 - Zinc alpha-2-glycoprotein as indicator of cancer: The present invention relates, in general, to methods of diagnosing and monitoring cancer and inflammatory diseases/disorders and, in particular, to methods of diagnosing and monitoring cancer and inflammatory diseases/disorders that comprise assaying for elevated levels of zinc alpha-2-glycoprotein (ZAG) in serum and other body fluids. The invention also relates to...

20060166285 - Charged permeation layers for use on active electronic matrix devices: An electronic device including at least one electrode on a substrate and a permeation layer overlaying the at least one electrode. The permeation layer comprises a polymer having a plurality of negatively-charged moieties. The negatively charged moieties may be carboxylates. In one embodiment, the negatively charged moieties are carboxylates from...

20060166286 - Methods and compositions for tagging via azido substrates: The invention provides methods and compositions for azide tagging of biomolecules. In one embodiment of the invention, proteins are tagged by metabolic incorporation of prenylated azido-analog substrates. Examples of such analogs are azido farnesyl diphosphate and azido farnesyl alcohol. The azido moiety in the resulting modified proteins provides an affinity...

20060166284 - Novel tissue factor targeted antibodies as anticoagulants: This invention relates to novel antibodies that bind with greater affinity to the factor VIIa/tissue factor (FVIIa/TF) complex than to tissue factor (TF) alone, do not compete for binding to TF with FVII and FX, an inhibit FX activation. The antibodies bind at the site of injury and prevent the...

20060166297 - High affinity anti-n¹,n¹²-diacetylspermine monoclonal antibody: The present invention provides an anti-diacetylspermine specific monoclonal antibody which satisfies a specific measuring condition in an immunoreaction system between a solid-phased diacetylspermine and the anti-diacetylspermine specific monoclonal antibody, wherein the immunoreaction system comprises a sample diacetylspermine or sample N1-acetylspermidine to inhibit the immunoreaction; and the specific measuring condition is...

20060166295 - Method for determination of likelihood of occurrence of preterm labor in pregnant females: The presence of 3-chlorotyrosine in vaginal secretions in a predictor of the likelihood preterm premature rupture of the fetal membranes, i.e., the chorioamnion and/or the occurrence of the risk of preterm labor. 3-chlorotyrosine is a marker for the excessive production of hypochlorous acid which causes focal areas of increased collagen...

20060166296 - Standard compound for immunoassay for dioxin and method of immunoassay for dioxin: e

20060166298 - Assay system for simultaneous detection and measurement of multiple modified cellular proteins: A method and kit for simultaneous detection and/or determination of a plurality of modified proteins in a sample. The method comprises: a) contacting the sample under mild protein denaturation conditions with a plurality of first antibodies capable of binding to a specific target protein, the first antibodies being immobilized on...

20060166299 - Proteins in type 2 diabetes: The present invention relates to the use of naturally occurring compounds and derivatives thereof as markers for predisposition of diabetes related diseases. The invention also relates to a pharmaceutical composition for treatment of the diabetes related diseases....

20060166300 - Method of measuring homocysteine: A method for detecting or measuring homocysteine in a sample includes the steps of (a) reacting a D-amino acid present in a sample with a D-amino acid converting enzyme to convert the D-amino acid into a substance that does not serve as a substrate of D-amino acid oxidase or D-amino...

20060166301 - Stable chromogenic test reagent and its use in coagulation-diagnostic tests: The present invention relates to a chromogenic test reagent which comprises a chromogenic peptide substrate and an inhibitor of fibrin polymerization, which is particularly suitable for being used in coagulation-diagnostic tests and which is distinguished by the fact that it exhibits a nigh degree of stability and/or a long shelf...

20060166302 - Handheld raman blood analyzer: Methods and apparatus for in vitro detection of an analyte in a blood sample using low resolution Raman spectroscopy are disclosed. The blood analyzer includes a disposable strip for receiving a sample of blood on a target region, the target region including gold sol-gel to provide surface enhanced Raman scattering....

20060166304 - Polynucleotides encoding insect 3-ketosphinganine reductase and uses thereof: The instant invention provides nucleic acids encoding insect 3-ketosphinganine reductase, as well as insect 3-ketosphinganine reductase encoded thereby. The invention further provides methods of identifying agents that modulate a level of 3-ketosphinganine reductase mRNA, polypeptide, or enzyme activity. Such agents are candidate insecticidal compounds....

20060166303 - Use of cardiac hormones for assessing a cardiovascular risk with respect to the administration of anti-inflammatory drugs: The present invention relates to the use of cardiac hormones, particularly natriuretic peptides, for diagnosing the cardiovascular risk of a patient who is a candidate for administration of a selective Cox-2 inhibitor, comprising the steps of a) measuring, preferably in vitro, the level of a cardiac hormone, b) diagnosing the...

20060166305 - Animal cell confluence detection method and apparatus: The invention provides an apparatus and process for detecting the degree of confluence of animal cells being cultured in a well plate. A well plate is arranged in an imaging station and illuminated with a ring of LEDs, or other optical source, from below at an oblique angle. An image...

20060166306 - Methods and compositions for in vitro synthesis of biological macromolecules in a cell-free system enriched with atp-sulfurylase: The present invention provides methods and composition for enhancing in vitro synthesis of biological macro-molecules in a call-free system where ATP is required as a primary energy source, wherein said cell-free system is enriched with ATP-sulfurylase The invention relates also to as cell-free systems and cell-free extracts enriched with ATP-sulfurylase...

20060166307 - Mixing devices for chemical lysis of cells: The invention relates to flow-through devices for mixing fluids and uses thereof for mixing and for lysing cells to release biological compounds of interest. The invention particularly encompasses flow-through methods for mixing and chemical methods for the isolation and purification of plasmid DNA from cell culture....

20060166308 - Stereoisomerically enriched beta-lactams using candida antarctica: The present disclosure provides enzymatic methods for generating stereoisomerically pure products by resolving a racemic mixture of N-protected β-lactams with a lipase from Candida antarctica with high stereospecificity. The presence of a carbamate protecting group, such as the tert-butoxycarbonyl group protecting group, on the β-lactam enhances enzyme catalysis and stereoselectivity....

20060166326 - 32142, 21481, 25964, 21686, novel dehydrogenase molecules and uses therefor: The invention provides isolated nucleic acids molecules, designated DHDR nucleic acid molecules, which encode novel DHDR-related dehydrogenase molecules. The invention also provides antisense nucleic acid molecules, recombinant expression vectors containing DHDR nucleic acid molecules, host cells into which the expression vectors have been introduced, and nonhuman transgenic animals in which...

20060166319 - Charging trna with pyrrolysine: Provided herein is a method for preparing a cell that, when exposed to the non-canonical amino acid, pyrrolysine or a derivative thereof and transformed or transfected with a polynucleotide comprising an in-frame UAG or TAG codon incorporates the pyrrolysine residue or derivative thereof into the protein or polynucleotide into the...

20060166323 - Compositions comprising promoter sequences and methods of use: Nucleic acid molecules, fragments and variants thereof having promoter activity are provided in the current invention. The invention also provides vectors containing a nucleic acid molecule of the invention and cells comprising the vectors. Methods for making and using the nucleic acid molecules of the invention are further provided....

20060166322 - Egvi endoglucanase and nucleic acids encoding the same: The present invention provides a novel endoglucanase nucleic acid sequence, designated egl6, and the corresponding EGVI amino acid sequence. The invention also provides expression vectors and host cells comprising a nucleic acid sequence encoding EGVI, recombinant EGVI proteins and methods for producing the same....

20060166325 - Glial cell line-derived neurotrophic factor receptor: The present invention relates to glial cell line-derived neurotrophic factor (GDNF), a potent neurotrophin that exhibits a broad spectrum of biological activities on a variety of cell types from both the central and peripheral nervous systems. The present invention involves the cloning and characterization of a high affinity receptor for...

20060166327 - Human g-protein receptor hgber32: Human G-protein coupled receptor polypeptides and DNA (RNA) encoding such polypeptides and a procedure for producing such polypeptides by recombinant techniques is disclosed. Also disclosed were methods for utilizing such polypeptides for identifying antagonists and agonists to such polypeptides and methods of using the agonists and antagonists therapeutically to treat...

20060166320 - In vitro expression of the hcv arfp/f-core‘coding open reading frame: A method of producing and purifying an HCV polypeptide, wherein the method comprises providing host cells containing an expression vector comprising a polynucleotide operably linked to expression control elements, wherein the polynucleotide is an open reading frame that overlaps the core gene in the +1 frame (core+1 ORF) of HCV,...

20060166314 - Method for rational mutagenesis of alpha/beta t-cell receptors and correspondingly mutated mdm2-protein specific alpha/beta t-cell ceceptores: The invention relates to the rational mutagenesis of polypeptides of α/β T-cell receptors that mediate an oncogen-specific T-cell response, nucleic acids encoding these and their use in the therapy, diagnosis and/or prevention of cancerous diseases. The invention further relates to a T-cell response-mediating MDM2-protein-specific α/β T-cell receptor, which has been...

20060166318 - Methods, compositions, and cells for encapsidating recombinant vectors in aav particles: Isolated recombinant polynucleotides comprising elements which promote encapsidation into AAV particles, packaging cells comprising the recombinant polynucleotides, and methods for their use are provided in the present invention. These isolated recombinant polynucleotides comprise a non-AAV ITR encapsidation element (such as the P1 sequence located within the AAV S1 integration site...

20060166309 - Novel g-protein coupled receptors: The invention provides isolated nucleic acid and amino acid sequences of four novel G-protein coupled receptors that are amplified in breast cancer cells, antibodies to such receptors, methods of detecting such nucleic acids and receptors, and methods of screening for modulators of G-protein coupled receptors....

20060166313 - Novel glycero kinase, gene thereof and process for producing the glycerol kinase by using the gene: Relating to a gene encoding a new glycerol kinase and a method for preparing the enzyme by gene recombination technique. A Glycerol kinase which has high resistance against preservative, a recombinant vector comprising a gene encoding the glycerol kinase, a transformant prepared by transforming a host cell with the recombinant...

20060166312 - Novel microorgnaism sensitive to lysozyme: A microorganism of the genus Rhodococcus is provided which has a higher sensitivity to lysozyme at a low concentration than a wild-type strain, which can easily cause cell lysis, and from which a recombinant protein expressed therein is easily recovered. More specifically, a mutant microorganism of the genus Rhodococcus having...

20060166311 - Novel notch-origin polypeptides and biomarkers and reagents using the same: It is intended to provide extracellular markers whereby Notch signal transduction can be detected. Polypeptides (Nβ), which are novel peptides originating in Notch protein and released form cells in the step of the nuclear migration of NICH (Notch intracellular cytoplasmic domain) due to the extracellular digestion and the subsequent protein...

20060166317 - Nucleic acids encoding sialytransferases from c. jejuni: This invention provides prokaryotic glycosyltransferases, including a bifunctional sialyltransferase that has both an α2,3- and an α2,8-activity. A β1,4-GalNAc transferase and a β1,3-galactosyltransferase are also provided by the invention, as are other glycosyltransferases and enzymes involved in synthesis of lipooligosaccharide (LOS). The glycosyltransferases can be obtained from, for example, Campylobacter...

20060166316 - Phosphodiesterase 10a7 isoforms and methods of use: The invention relates to mammalian phosphodiesterase PDE10A7 isoforms, as well as the nucleic acids encoding such polypeptides. The invention further relates to methods for utilizing such polypeptides in diagnostic assays and in methods of screening potential modulators, especially inhibitors, of the novel PDE10A7 disclosed herein. The polypeptides of the invention...

20060166315 - Process for producing pnpase: The invention provides a process for producing PNPase, wherein PNPase can be produced easily with high efficiency, and problematic contamination with endotoxin in synthesis of a nucleic acid polymer as a raw material of pharmaceutical preparations can be reduced. PNPase is produced by Escherichia coil or the like having a...

20060166321 - Recombinant influenza vectors with tandem transcription units: The invention provides a composition useful to prepare influenza viruses, e.g., in the absence of helper virus, using vectors which include tandem transcription cassettes containing PolI and/or PolII promoters....

20060166324 - Screening tools for discovery of novel anabolic agents: The disclosure provides nucleic acids including the polynucleotide sequence of the human MAFbx core promoter involved in muscle specific expression. Also provided are reporters operably linked to a polynucleotide sequence including MAFbx transcription regulatory sequences, and constructs including polynucleotides that encode reporters and other polynucleotide sequences operably linked to the...

20060166310 - Serotonin receptor: This invention relates to a protein, termed INPIONCH1, herein identified as a 5-HT3 receptor and to the use of this protein and nucleic acid sequence from the encoding gene in the diagnosis, prevention and treatment of disease....

20060166328 - Igf-i fusion polypeptides and therapeutic uses thereof: A fusion protein comprising at least one IGF1 variant component and a fusion component (F), and, optionally, a signal sequence, exhibiting improved stability relative to the native IGF1 or IGF2 polypeptide. The fusion component (F) may be a multimerizing component, a targeting ligand, or another active or therapeutic compound. IGF1...

20060166329 - Albumin fusion proteins: The present invention encompasses albumin fusion proteins. Nucleic acid molecules encoding the albumin fusion proteins of the invention are also encompassed by the invention, as are vectors containing these nucleic acids, host cells transformed with these nucleic acids vectors, and methods of making the albumin fusion proteins of the invention...

20060166330 - Process for producing purified anthocyanin and crystalline anthocyanin: Provided are a process for producing purified anthocyanidin glucoside in which a rhamnose end of anthocyanidin rutinoside is cleaved using rhamnosidase to convert the anthocyanidin rutinoside component into anthocyanidin glucoside, the anthocyanidin glucoside component being then purified and isolated; or a crystalline anthocyanidin glucoside salt obtained by further crystallizing the...

20060166331 - Apparatus and method for preparative scale purification of nucleic acids: Apparatus and methods are described for pharmaceutical grade manufacture extrachromosomal nucleic acids from cell lysates using flotation to separate and eliminate undesired insoluble cellular debris including chromosomal DNA from the lysates. A gas is introduced to controllably generate bubbles that reduce the density of the cell debris and create a...

20060166332 - Method for ligating nucleic acids and molecular cloning: The invention provides cells and methods of circularizing linear DNA molecules. The cell is an isolated Escherichia coli cell which transiently expresses the Cre recombinase protein from an integrated Cre recombinase gene, and which is at least transiently repressed for RecBCD activity. The cells are used in a method of...

20060166334 - Method and compositions for rapidly modifying clones: A method for removing extraneous nucleotides in a cloned coding sequence using a type IIs endonuclease, the method comprising introducing a linker that comprises at least one recognition site for a Type IIs restriction endonuclease to the ORF, cloning the ORF into a suitable vector, and removing the extraneous nucleotides...

20060166333 - Methods of collecting and transporting vaginal discharge for detection of infectious organisms and to facilitate cervical cancer screening: The present invention provides a method of testing for the presence of infectious disease agents or host genetic markers comprising applying a device comprising an absorbent and porous material onto the introitus of a female patient; encouraging air drying of at least a portion of the collected vaginal discharge while...

20060166335 - Glucuronofucan sulfate: Three enzymes degrading sulfated glucuronofucan; processes for producing these enzymes; activators for these enzymes; glucuronofucan sulfate and degradation products thereof; and a novel microorganism....

20060166336 - Modified alternan: Four new Penicillium spp. isolates (NRRL 21966, NRRL 21967, NRRL 21968, and NRRL 21969) are capable of essentially quantitative conversion of native alternan to a polymeric modified form having a lower apparent molecular weight than native alternan. A fifth isolate (NRRL 30489) obtained from a survey of deposited organisms and...

20060166338 - Alleles of the mqo gene from coryneform bacteria: The invention relates to mutants and alleles of the coryneform bacterium mqo gene which encodes malate quinone oxidoreductases which contain any amino acid apart from L-serine at position 111, or a comparable position, in the amino acid sequence, and to processes for fermentatively preparing amino acids, preferably L-lysine, L-tryptophan and...

20060166337 - Process for producing optically active erythro 3-cyclohexylserines: (wherein, R1 means an alkanoyl group having 1-10 carbons, a benzoyl group, a halogen-substituted alkanoyl group having 1-5 carbons or a halogen-substituted benzoyl group, and R2 means a phenyl group or a cyclohexyl group) is subjected to asymmetrical hydrolysis with L-aminoacylaseorD-aminoacylase, there by giving unreacted N-acyl-D-erythro3-substituted serine in the case...

20060166340 - Microorganism and the process for preparation of pravastatin sodium: The invention provides a novel microorganism producing pravastatin sodium, as well as the method for producing pravastatin sodium by using this microorganism. Micropolyspora roseoalba CGMCC 0624 of the invention is highly tolerant to mevastatin sodium, and has a high transformation rate of mevastatin sodium, and can produce pravastatin sodium with...

20060166339 - Production of l-aldonolactone: The present invention provides a process for the production of L-aldonolactone from L-aldohexose, especially for producing L-gulono-1,4-lactone or L-gulonic acid from L-gulose and producing L-galactono-1,4-lactone or L-galactonic acid from L-galactose by a microorganism belonging to the genus Pseudomonas or the genus Gluconobacter....

20060166341 - Method for reproducing pleuromutilins: Invented are non-peptide TPO mimetics. Also invented is a method of treating thrombocytopenia, in a mammal, including a human, in need thereof which comprises administering to such mammal an effective amount of a selected hydroxy-1-azobenzene derivative....

20060166342 - Process for producing optically active 3-hydroxypropionic ester derivative: The present invention is to provide a process for simply producing an optically active 3-hydroxypropionic ester derivative useful as a medicament intermediate from an inexpensive material. More specifically, the present invention is directed to a process for producing an optically active 3-hydroxypropionic ester derivative comprising reacting an acetic ester derivative...

20060166343 - Photosynthetic hydrogen production: A process for the production of hydrogen, comprising the steps of: (i) providing a photosynthetic microorganism having electron transfer capability through a photosynthetic “light” reaction pathway and through a respiratory electron transfer chain involving an oxidative phosphorylation pathway, and which expresses a hydrogenase, wherein regulation of the oxidative phosphorylation pathway...

20060166344 - Bacterial outer memberane vesicles: Existing methods of meningococcal OMV preparation involve the use of detergent during disruption of the bacterial membrane. According to the invention, membrane disruption is performed substantially in the absence of detergent. The resulting OMVs which retain important bacterial immunogenic components, particularly (i) the protective NspA surface protein, (ii) protein NMB2132...

20060166345 - Method of inactivating enveloped viruses in a viral preparation of non-enveloped viruses: The present invention relates to a method of inactivating enveloped viruses in a viral preparation predominantly containing non-enveloped viruses by the action of a solvent at a temperature of between −5° C. and +50° C. and at a pH of between about 5 and 9. Its subject is also a...

20060166346 - Support for holding a complexed enrichment of degrading bacteria and manufacturing method thereof, novel bacteria, and method of cleaning pollution environment and device thereof: The degrading bacteria A may be a PCNB-degrading bacteria, particularly degrading bacteria containing degrading bacteria having part or all of the bacteriological characteristics of Nocardioides sp. PD653 and the degrading bacteria B may be degrading bacteria containing degrading bacteria having part or all of the bacteriological characteristics of β-Proteobacteria CDB21....

20060166347 - Sample preparation devices and methods: A method and device for preparing biological samples includes lysing the microorganisms, and collecting a desired biological material....

20060166348 - Anaerobic biological degradation of hydrocarbons: The invention relates to a method for the anaerobic biological degradation of aromatic hydrocarbons (in particular benzene), and to a specific mixture and the use thereof for this degradation. According to the invention, the anaerobic biological degradation of aromatic hydrocarbons present at a contaminated location is stimulated and stabilized by...

20060166349 - Recovery of plasmids in an aqueous two-phase system: The present invention is a method for the purification of plasmid DNA comprising to provide a composition comprising a first polymer having inverse solubility characteristics and a second polymer immiscible in the first polymer; contacting said solution with an aqueous solution comprising plasmid DNA; providing phase separation and isolating the...

20060166351 - Cell capturing apparatus and method of capturing cell: A path defines an opening smaller than a cell in a cell capturing apparatus. A negative pressure generating device is connected to the path so as to generate negative pressure within the path. A negative pressure controlling device is connected to the path so as to adjust the negative pressure...

20060166352 - Device and method for analyte detection: The invention is directed to a method and device for simultaneously testing a sample for the presence, absence, and/or amounts of one or more of a plurality of selected analytes. The invention includes, in one aspect, a device for detecting or quantitating a plurality of different analytes in a liquid...

20060166350 - Method of detecting an analyte in a fluid: A method for the detection of an analyte in a fluid, comprising contacting the fluid with a holographic element comprising a medium and a hologram disposed throughout the volume of the medium, wherein an optical characteristic of the element changes as a result of a variation of a physical property...

20060166353 - Process for realishing a biomorphic, stereolithographed phantom, which is multicompartmental and suitable for multanalytical examinations, and relevant device: A process for preparing digital images for realising a biomorphic multicompartmental phantom, includes a phase A.1 of acquisition of images of the organ belonging to the analysed living being, forming a volumetric image defined by voxels, a phase A.2 of identification of tissues and/or tissue liquids and a phase B...

20060166354 - Bioreactors with multiple chambers: A bioreactor for cultivating living cells in a liquid medium. In one embodiment of the present invention, the bioreactor has a first substrate having a first surface and an opposite second surface, defining a chamber therebetween for receiving the cells and the liquid medium. The bioreactor further has a barrier...

20060166355 - Imaging fluorescence signals using telecentric optics: The present invention relates to the field of DNA analysis. In particular, the present invention is directed to a device for the parallel imaging of fluorescence intensities at a plurality of sites as a measure for DNA hybridization. More particular, the present invention is directed to a device to image...

20060166358 - Biofilm incubation: Biofilm forming organisms are incubated to form a biofilm on projections by providing a flow of liquid growth medium across projections, the direction of the flow of liquid being repeatedly changed, and an assay made of the resulting biofilm. Biofilm forming organisms are incubated to form a biofilm on projections...

20060166356 - Cell culture device: The invention concerns a device designed in particular for cell culture (10) comprising a chamber (100) wherein are arranged the cells (10), said chamber (100) comprising at least one first conduit (131, 141, 151, 161, 133, 143, 153, 163) for injecting a first type of fluid (M1), at least a...

20060166357 - Integrated microfludic control employing programmable tactile actuators: Microfluidic devices having active features such as valves, peristaltic pumps, and mixing portions are fabricated to have a thin elastomeric membrane over the active features. The active features are activated by a tactile actuator external to the membrane, for example, a commercial Braille display. The display may be computer controlled,...

20060166359 - Transgenic mice containing lumulus clotting factor protease-like gene disruptions: The present invention relates to transgenic animals, as well as compositions and methods relating to the characterization of gene function. Specifically, the present invention provides transgenic mice comprising mutations in a limulus clotting factor protease-like gene. Such transgenic mice are useful as models for disease and for identifying agents that...

20060166360 - Compositions, solutions, and methods used for transplantation: This invention discloses a method for reducing the intracellular lipid storage material of a cell, tissue, or organ for transplantation and features solutions, methods and kits that induce the metabolic elimination of lipid storage in a cell, tissue, or organ. In one exemplary approach, the process involves contacting a cell,...

20060166361 - Postpartum cells derived from placental tissue, and methods of making, culturing, and using the same: Cells derived from postpartum placenta and methods for their isolation are provided by the invention. The invention further provides cultures and compositions of the placenta-derived cells. The placenta-derived cells of the invention have a plethora of uses, including but not limited to research, diagnostic, and therapeutic applications....

20060166362 - Method of inducing differentiation of bone marrow stromal cells to neural cells or skeletal muscle cells by introduction of notch gene: There is provided a method of inducing differentiation of bone marrow stromal cells to neural cells or skeletal muscle cells by introduction of a Notch gene. Specifically, the invention provides a method of inducing differentiation of bone marrow stromal cells to neural cells or skeletal muscle cells in vitro, which...

20060166363 - Modified baculovirus expression system for production of pseudotyped raav vector: The invention provides modifications to a baculovirus-based recombinant adeno associated virus (AAV) system including enhancement of the helper virus stability and construction of novel baculovirus vectors for rAAV pseudotyping. The modified system extends the flexibility of rAAV vector production and promotes the utility of AAV as, a clinically applicable gene...

20060166364 - Use of flexible bag containers for viral production: The present invention relates generally to the fields of cell banking and viral production. More particularly, it concerns a method of virus production from host cells using flexible containers....

20060160061 - Dehydration procedures for induction of cryptobiosis in insect larvae: This invention provides a method for effectively producing dehydrated larvae for educational materials without disrupting the environment. The cryptobiotic larvae for educational materials can be obtained by dehydrating larvae while gradually reducing humidity in 3 separate stages....

20060160062 - Perfusion and/or preservation solution for organs: The present invention relates to a solution for preservation, perfusion, and/or reperfusion of an organ, especially the heart, for transplantation. The solution contains peptide inhibitor(s) of protein kinase C βII (PKC βII) and/or of protein kinase C ζ (PKC ζ) and/or peptide activator(s) of protein kinase C δ (PKCδ). Methods...

20060160066 - Cellular microarrays for screening differentiation factors: Provided is a microarray platform for the culture of cells atop combinatorial matrix mixtures; enabling the study of differentiation in response to a multitude of microenvironments in parallel....

20060160064 - Devices and methods for removing target agents from a sample: The invention provides devices, test kits and methods for removing target agents from a sample. The device contains one or more porous matrices having pore sizes larger than 10 μm, and a plurality of particles impregnated therein. The target agents attach the device and are removed from the sample....

20060160065 - Method for classifying plant embryos using raman spectroscopy: A three-step method for classifying plant embryo quality using Raman spectroscopy is provided. First, a classification model is developed based on Raman spectral data of reference samples of plant embryos or any portions of plant embryos of known embryo quality. The embryo quality may be known based on a comparison...

20060160063 - Method of screening physilogically active substance: The present invention relates to a method of screening a physiologically active substance, comprising the steps of (1) contacting a transformed yeast with a test sample, wherein the transformed yeast is capable of expressing a heterogeneous protein, and shows a change in a growing state in an expression state of...

20060160070 - Association-based epitome design: Systems that facilitate immunogen design are described herein. An optimization component is provided to determine an immunogen according to at least one criterion. The immunogen comprises a set of overlapping sequences comprising sequences that are known to be and/or are likely to be immunogenic. At least one of the sequences...

20060160071 - Association-based predictions: A system comprising a machine learning classifier trained on a plurality of associations between a host and a pathogen to predict a pathogen characteristic is described herein. The pathogen characteristic can relate to a disease state of the host. Computer-executable instructions for performing a method of forecasting a portion of...

20060160067 - Chimeric gb virus b (gbv-b): The present invention relates generally to the fields of biochemistry, molecular biology, and virology. More particularly, it relates to the identification of GB virus B (GBV-B)/HCV chimeras. The invention involves nucleic acid constructs and compositions encoding GBV-B/HCV chimera, including at least part of a 5′ NTR derived from a HCV...

20060160068 - Competitive immunoassay: The present invention provides ligand-detection reagents, ligand analogs and methods for determining the presence of a ligand in a sample. The ligand-detection reagent comprises a ligand-binding antibody and a ligand analog to form an antibody-ligand analog complex wherein the ligand analog is covalently bonded to a reporter molecule. This complex...

20060160073 - Efficient algorithm for pcr testing of blood samples: Systems, processes, and devices are provided which are useful for testing blood or plasma donations to detect those specific donations which are contaminated by a virus above a predetermined level. An apparatus and process is described which forms individual, separately sealed and connected sample containers from a flexible hollow tubing...

20060160072 - Method for molecular cloning and polynucleotide synthesis using vaccinia dna topoisomerase: This invention provides a modified vaccinia topoisomerase enzyme containing an affinity tag which is capable of facilitating purification of protein-DNA complexes away from unbound DNA. This invention further provides a modified seauence specific topoisomerase enzyme. This invention provides a method of ligating duplex DNAs, a method of molecular cloning of...

20060160069 - Methods and compositions for the diagnosis of cancer: In one embodiment, the invention relates to a method of detecting cervical cancer, and other types of cancer, using a combination of at least three genomic clones, or fragments thereof, of high risk Human Papilloma Virus. For example, the invention relates to a composition comprising at least three full length...

20060160126 - 90 human secreted proteins: The present invention relates to novel human secreted proteins and isolated nucleic acids containing the coding regions of the genes encoding such proteins. Also provided are vectors, host cells, antibodies, and recombinant methods for producing human secreted proteins. The invention further relates to diagnostic and therapeutic methods useful for diagnosing...

20060160115 - Activation method of protein derived from extremely thermophilic bacterium in nucleic acid amplification reaction and use thereof: The present invention is to provide a method which makes it possible to activate RecA, and maintain its biological function in all the PCR cycles. Also, the invention is to provide a nucleic acid amplification method, and a kit for amplifying a nucleic acid which makes it possible to suppress...

20060160092 - Adip protein and use thereof: The objective of the present invention is to provide a gene encoding novel afadin DIL domain-binding protein (ADIP) and use of the novel ADIP protein. The present inventors successfully identified a novel afadin-binding protein (ADIP) using yeast two-hybrid screening in order to identify how the nectin and afadin system organize...

20060160097 - Amplification reaction vessel, and method of manufacturing the same: A nucleic acid amplification reaction vessel has a substrate, a cavity formed in the substrate, a cover plate for sealing the cavity, and a sample-injection inlet formed in the cover plate. The cavity includes a columnar structure connected to the cover plate or the substrate. The nucleic acid amplification reaction...

20060160080 - Avian sex determination method: A method for the determination of the sex of an avian subject is provided which comprises analysis of a sample from said subject with a nucleic acid probe comprising an at least 6 base pair fragment from a Female Associated Factor (FAF) nucleic acid sequence, or with an antibody to...

20060160090 - Composition splice variants and methods relating to cancer specific genes and proteins: The present invention relates to newly identified nucleic acid molecules and polypeptides present in normal and neoplastic cells, including fragments, variants and derivatives of the nucleic acids and polypeptides. The present invention also relates to antibodies to the polypeptides of the invention, as well as agonists and antagonists of the...

20060160125 - Compositions and methods for nonenzymatic ligation of oligonucleotides and detection of genetic polymorphisms: The invention is directed to novel compositions and methods for nonenzymatic ligation of oligonucleotides. In one aspect of the invention, the nonenzymatic ligation is selenium-mediated or tellurium mediated ligation. In another aspect, the invention provides for the use of fluorescence resonance energy transfer (FRET) to detect the nonenzymatic ligation....

20060160124 - Detection of negatively charged polymers using water-soluble, cationic, polythiophene derivatives: Novel methods allowing for the simple optical and electrochemical detection of double-stranded oligonucleotides are disclosed. The methods are rapid, selective and versatile. Advantageously, they do not require any chemical reaction on the probes or on the analytes since they are based on different electrostatic interactions between cationic poly(3-alkoxy-4-methylthiophene) derivatives and...

20060160083 - Diagnositics and therapeutics for diseases associated with human g-protein couple receptor 14 (gpr14): The invention provides a human GPR14 which is associated with the hematological diseases, cardiovascular diseases, disorders of the peripheral and central nervous system, urological diseases, endocrinological diseases and cancer. The invention also provides assays for the identification of compounds useful in the treatment or prevention of hematological diseases, cardiovascular diseases,...

20060160088 - Diagnostics and therapeutics for diseases associated with g protein-coupled receptor