Chemistry: molecular biology and microbiology inventions 07/06

20060166182 - Tissue and organ preservation, protection and resuscitation: The present invention provides compositions and methods for protecting tissues and organs from damage during transplantation or from acute ischemia due to, e.g., injury or surgery. The compositions protect the tissue or organ from acidosis, oxidative damage, ischemia and repurfusion injury while the organ is isolated from the normal circulation...

20060166184 - Apparatus for cell reaction examination and method of cell reaction examination: Disclosed herein are an inspection apparatus for cell reaction by a device for liquid processor, which can set a fluid running route to a cell reaction site required according to the kind of an inspection of a cell reaction to be conducted with a great degree of freedom, and an...

20060166183 - Preparation of coatings through plasma polymerization: The invention provides a method to prepare at least part of at least one surface of a substrate comprising; depositing on said surface at least one plasma monomer wherein during deposition of said monomer, means are provided which move the monomer source across a surface to be treated to manufacture...

20060166185 - System for and method of patch clamp analysis: A system and method for automated patch clamp analysis of biological tissue such as cellular membrane. A biological membrane is applied to a porous semiconductor substrate, and laser energy is imparted thereon. Voltage clamped electrodes detect current across the membrane to indicate ion channel activity....

20060166186 - Akap84 and its use for visualization of biological structures: A polynucleotide encoding a chimeric polypeptide is provided. The chimeric polypeptide includes (a) a first polypeptide region being capable of specifically binding at least one detectable molecule; and (b) a second polypeptide region being capable of specifically binding a biological component or macromolecule or targeting a cellular compartment. Methods utilizing...

20060166193 - Binding protein: HCV assays are described. The assays utilize a 24kd protein capable of binding the E2 envelope protein of hepatitis C virus (HCV), or functionally equivalent variants or fragments of the 24kd protein....

20060166192 - Detection of protease-resistant prion protein following a spontaneous transformation reaction: The present invention relates to a method for detecting infectious or pathogenic prion protein with an improved degree of sensitivity. For this, protease-resistant prion protein PrPSc is formed de novo by means of a spontaneous transformation reaction, with nonpathogenic prion protein PrPc in the sample interacting with protease-sensitive low molecular...

20060166187 - Hbv precore protein capable of forming particles: There is provided a HBV precore protein having an ability of forming particles, and a means for determining it. A novel HBV precore protein that forms the virus (like) particles of HBV was identified. The present invention provides this novel HBV precore protein. Furthermore, there are provided core-like particles and...

20060166191 - Inhibition of ap-3/gag interactions in the treatment of human immunodeficiency virus infections: The present invention provides for methods of identifying potential inhibitors of HIV infection and replication. More specifically, the invention identifies complexes between the δ subunit of AP-3 and Gag, which facilitate HIV assembly. Screening for inhibitors of this interaction will identify lead compounds for the treatment of HIV and AIDS....

20060166190 - Magnetism based nucleic acid amplification: This invention relates generally to the field of nucleic acid amplification. In particular, the invention provides processes and kits for amplifying a nucleic acid of a target cell or virus using, using inter alia, binding between a target cell, cellular organelle or virus with a magnetic microbead....

20060166189 - Methods for detecting invasion of a cell: The present invention provides a rapid method to determine invasion of a cell by an invasion....

20060166188 - Methods of examining swine genetic resistance to rna virus-origin disease: The inventors investigated the impact of an 11-bp deletion in the swine Mx1 gene on the ability to suppress propagation of influenza viruses belonging to the myxovirus family, and revealed that the deletion led to a complete loss of the ability to suppress viral propagation. Through the detection of the...

20060166236 - Allosteric enzyme coupled immunoassay (aecia): The present invention is directed to methods and compositions for an allosteric enzyme coupled assay, and preferably to an allosteric enzyme coupled immunoassay (AECIA). The assay uses an allosteric enzyme to generate a readout signal. The assay is based on the competition between an analyte in a sample and an...

20060166259 - Apm1 biallelic markers and uses thereof: The invention provides novel APM1 genomic sequences, polypeptides, antibodies, and polynucleotides including biallelic markers derived from the APM1 locus. Primers hybridizing to regions flanking these biallelic markers are also provided. This invention also provides polynucleotides and methods suitable for genotyping a nucleic acid containing sample for one or more biallelic...

20060166234 - Apparatus and system having dry control gene silencing compositions: An RTF testing plate can include at least a first control well including a substantially dry first control composition having at least a first control siRNA. The first control siRNA is capable of providing a first indication of the gene silencing efficacy. Additionally, the first control composition can be configured...

20060166215 - Assay and process for labeling and detection of micro rna and small interfering rna sequences: A process for protecting a short RNA fragment includes labeling a short RNA fragment with a detectable platinum compound forming a labeled small RNA fragment. Resulting labeled short RNA fragment is exposed to a capture oligonucleotide. The capture oligonucleotide includes at least two replicates of a nucleotide sequence complimentary to...

20060166224 - Associations using genotypes and phenotypes: The present invention discloses methods for combining data on genetic variations and phenotypes of individuals to predict a phenotype-of-interest. The present invention also discloses kits that can be used to determine if an individual has or does not have a phenotype-of-interest. The kit can include at least one diagnostic tool...

20060166216 - Biochemical reaction system, biochemical reaction substrate, process for producing hybridization substrate and hybridization method: A bioassay substrate (1) is flat and has a disc-shaped main side like an optical disc such as CD. The substrate (1) is rotatable about a central hole (2) formed therein. The substrate (1) has formed on the surface (1a) thereof a plurality of wells (8) where a probe-use DNA...

20060166212 - Breast specific protein expressed in cancer and methods of use thereof: Cancer-linked gene sequences, and derived amino acid sequences, are disclosed along with processes for assaying potential antitumor agents based on their modulation of the expression of these cancer-linked genes. Also disclosed are antibodies that react with the disclosed polypeptides and methods of using the antibodies to treat cancerous conditions, such...

20060166197 - Building block capable of transferring a functional entity: A building block having the dual capabilities of transferring the genetic information e.g. by recognising an encoding element and transferring a functional entity to a recipient reactive group is disclosed. The building block can be designed with an adjustable transferability taking into account the components of the building block. The...

20060166220 - Chimeric tumor suppressor gene and protein: The present invention relates to chimeric molecules comprising portions of rat PEG-3 (“rPEG-3”) and human GADD34 (“hGADD34”) having apoptotic activity. It is based, at least in part, on the discovery that a chimeric protein comprising amino acids 1-347 of rat PEG-3 fused with residues 418-674 of human GADD34 exhibited anti-proliferative...

20060166200 - Compositions of solution for sequencing reaction clean-up: Wash solution and method for purifying sequencing reaction product. The wash solution comprises an effective amount of guanidine in a low ionic solution to reduce or eliminate the presence of dye terminators in a sequencing reaction product In its method aspects, the present invention comprises the addition of the wash...

20060166257 - Converting diploidy to haploidy for genetic diagnosis: Detection of mutations associated with hereditary diseases is complicated by the diploid nature of mammalian cells. Mutations present in one allele are often masked by the wild-type sequence of the other allele. Individual alleles can be isolated from every chromosome within somatic cell hybrids generated from a single fusion. Nucleic...

20060166205 - Detecting the risk of cardiovascular disease by detecting mutations in genes, including genes encoding a2b-adrenoceptor and apoliporotein b: The present invention provides a method of identifying subject's susceptibility to cardiovascular diseases or risk of developing myocardial infarction (MI) or cerebrovascular stroke by detecting gene polymorphisms and other gene mutations from a biological sample of the subject and optionally obtaining information concerning the family and medical history, blood, serum,...

20060166246 - Detection of fungal pathogens using the polymerase chain reaction: The present invention relates to the use of primers in polymerase chain reaction assays for the detection of fungal pathogens Colletotrichum acutatum, Ateniaria spp., and Cladosporium carpophilum. Specific primers are identified as being useful for the identification of fungal isolates using PCR based techniques. Also described are novel extraction buffer...

20060166194 - Diagnosis and treatment of cancer: A method of diagnosing cancer in a human patient comprising the steps of (i) obtaining a sample containing nucleic acid and/or protein from the patient; and (ii) determining whether the sample contains a level of hNeNa voltage-gated Na+ channel nucleic acid or protein associated with cancer. A method of treating...

20060166226 - Dna amplification device: At the same time, at least the thickness Ld of regions Xc . . . situated under the cells C . . . in the lower substrate 7 is selected to be 1.0 [mm] or thinner, and, a thermo-module(s) comes into contact with the lower surface of the base 5....

20060166244 - Dna markers for increased milk production in cattle: The invention provides methods for identifying a genetic polymorphism associated with altered milk production traits in dairy cattle. Genetic marker-assisted selection methods provided by the invention allow avoidance of potentially costly phenotypic testing and inaccuracies associated with traditional breeding schemes and improvement of dairy cattle herds....

20060166223 - Dna purification and analysis on nanoengineered surfaces: A microfluidic device for isolating genomic DNA from human leukocytes, simultaneously capturing and measuring the DNA for simplifying genetic analysis. The device contains a fluid inlet port, a fluid outlet port, and a DNA binding channel in contact with the fluid inlet port wherein at least a portion of at...

20060166264 - Effect of comt genotype on frontal lobe function: The invention provides a method of detecting impaired prefrontal cognitive function in an individual by determining the individual's COMT genotype, and associating a high activity Val allele with impaired prefrontal cognitive function (and a low activity Met allele with enhanced prefrontal cognitive function)....

20060166248 - Fluorescent n2, n3-etheno-purine (2'deoxy) riboside derivatives and uses thereof: The invention relates to fluorescent N2,N3-etheno-purine (2′-deoxy) riboside derivatives and fluorescent oligonucleotide probes comprising one or more moieties thereof, their preparation and uses thereof for staining DNA/RNA and for detection and quantitation of genetic material....

20060166217 - Genetic marker for coronary artery disease: The present invention describes a Coronary artery disease (CAD) susceptibility gene and the use of this gene in the development of novel therapies to treat CAD and in the diagnosis of susceptibility to CAD....

20060166239 - Genetic variants predicting warfarin sensitivity: We discovered that a polymorphism in the promoter of the VKORC1 gene is associated with warfarin sensitivity. This polymorphism can explain both the inter-individual and inter-ethnic differences in warfarin dose requirements. Furthermore, the polymorphism is also associated with promoter activity. Thus, the promoter sequence or activity of the VKORC1 gene...

20060166209 - Growth hormone variations in humans and its uses: The present invention relates to a naturally-occurring growth hormone mutation; to a method for detecting it and its use in screening patients for growth hormone dysfunction or for producing variant proteins suitable for treating such irregularities....

20060166247 - High throughput assay for cancer cell growth inhibition: A high-throughput, anchorage-independent assay is described, which screens compounds for inhibition of cancer cell growth. The assay utilizes a three-dimensional matrix or semi-solid media transfected with the subject compound, and enables live colony growth determination and imaging....

20060166207 - Hsan ii related gene and expression products and uses thereof: Methods of utilizing a gene related to pain perception, herein dubbed HSN2, or its encoded protein, dubbed herein “sensorin,” for the screening and identification of agents for the treatment of pain, neuropathy and related disorders, especially small organic compounds, as well as methods of using these compounds to treat or...

20060166256 - Il-1 receptor accessory protein: DNA encoding IL-1R AcP splice variant polypeptides, the polypeptides and methods for using the encoded polypeptides are disclosed....

20060166208 - Induction of the mitochondrial permeability transition: The present invention relates to process for identifying a compound which induces the mitochondrial permeability transition (MPT) in proliferating cells, wherein said process comprises contacting a cell or cell extract with a compound, determining whether compound binds to adenine nucleotide translocator (ANT), and determining whether the compound selectively induces the...

20060166232 - Internal control nucleic acid molecule for nucleic acid amplification systems: The invention provides an internal control nucleic acid molecule including at least one forward primer binding site, at least one reverse primer binding site, and at least one amplifiable region, wherein the forward primer binding site, the reverse primer binding site, and the amplifiable region are all randomly generated. The...

20060166250 - Isothermal dna amplification: The present invention provides for amplification of one or more polynucleotides by multi-staged linear amplifications using one or more RNA polymerases. At each stage RNA transcripts are accumulated at a linear rate, so that multiple stages provide for faster than linear transcript accumulation. In one aspect, the invention provides for...

20060166202 - Lipid phosphate phosphatases and uses thereof for treating neuronal diseases: Lipid phosphate phosphatase proteins, genes coding for them, vectors and cells comprising them, antibodies directed against them, methods of identifying compounds binding to them and functional interactors as well as to the use of proteins, genes, vectors, cells, interacting compounds and functional interactors for treating neuronal diseases and/or injuries....

20060166214 - Marker for detecting mesenchymal stem cell and method of distinguishing mesenchymal stem cell using the marker: The present invention provides a marker for detecting, separating/distinguishing a mesenchymal stem cell and a method for detecting, separating/distinguishing a mesenchymal stem cell by using the marker. A gene shown in the sequence listing is expressed specifically in a mesenchymal stem cell. The present invention comprises use of the gene...

20060166228 - Markers of alterations in the y chromosome and uses therefor: Novel sequence tagged sites (STSs), probes and primers useful, e.g., for detecting the presence or absence of an STS in a sample, and methods of using these STSs, probes and primers, e.g., in methods of detecting alterations in the Y chromosome are disclosed. These compositions are also useful in methods...

20060166242 - Markers of pre-term labor: The invention relates to novel markers of pre-term labor, methods for assessing the status of pre-term labor using the markers, and methods for the diagnosis and therapy of pre-term labor....

20060166233 - Method and apparatus for processing polynucleotide-containing samples: Methods and systems for processing polynucleotides (e.g., DNA) are disclosed. A processing region includes one or more surfaces (e.g., particle surfaces) modified with ligands that retain polynucleotides under a first set of conditions (e.g., temperature and pH) and release the polynucleotides under a second set of conditions (e.g., higher temperature...

20060166229 - Method enabling use of extracellular rna extracted from plasma or serum to detect, monitor or evaluate cancer: This invention relates to the use of tumor-derived or associated extracellular ribonucleic acid (RNA) found circulating in the plasma or serum fraction of blood for the detection, monitoring, or evaluation of cancer or premalignant conditions. Extracellular RNA may circulate as non-bound RNA, protein-bound RNA, lipid-RNA complexes, lipoprotein (proteolipid)—RNA complexes, protein-RNA...

20060166195 - Method for detecting and identifying the presence of biological materials derived from fish and oligonucleotides therefor: A subject of the present invention is a method for detecting the presence of biological materials originating from gadiformes chosen from the group constituted by the gadidae, the merluccidae, the macrouridae and/or the moridae, in a sample of organic material, characterized in that the presence of mitochondrial DNA originating from...

20060166198 - Method for in vitro molecular evolution of protein function: A method for in vitro molecular evolution of protein function The invention provides a method for generating a polynucleotide sequence or population of sequences from parent single-stranded polynucleotide sequences encoding one or more protein motifs, comprising the steps of (a) providing a first population of single-stranded polynucleotide molecules and a...

20060166201 - Method for the detection of nucleic acid sequences by means of crackable probe molecules: The present invention describes a method for the detection of nucleic acid sequences, which is characterized in that the following steps are conducted: a) at least one nucleic acid is bound to a solid phase; b) probe molecules are hybridized to the nucleic acids in a sequence-specific manner, whereby the...

20060166237 - Method of detecting reaction of dna and dna-binding protein: In the case of detecting whether or not a binding site of a transcription factor TFIID and a transcription factor TFIIB is present in a labeled DNA, the presence of a binding site can be detected precisely by adding all of a transcription factor TFIID, a transcription factor TFIIB and...

20060166240 - Method of optimizing amplification in pcr: The present invention discloses a method for optimizing PCR amplification by adding elementary substance material into PCR system, wherein the elementary substance material is selected from a group consisting of element titanium, element nickel, element bismuth, element stibium, element selenium, element chromium, and a mixture of the group. This new...

20060166210 - Methodology of estimating the conformation of a protein by proteolysis: The invention relates to a method for determining the effect of a polymorphism or mutation on the structural properties of a protein wherein the method relies on the structural properties of the protein and so its cleavage during proteolysis....

20060166206 - Methods and compositions for analysis of regulatory sequences: Methods for constructing arrays of regulatory sequences, and the arrays so obtained, are provided. Regulatory sequences for use on the arrays are isolated based on their accessibility in cellular chromatin. A number of methods for using the arrays are disclosed, including regulatory DNA profiling, epigenome profiling, toxicological profiling and identification...

20060166225 - Methods and compositions for polypeptide engineering: Methods are provided for the evolution of proteins of industrial and pharmaceutical interest, including methods for effecting recombination and selection. Compositions produced by these methods are also disclosed....

20060166249 - Methods for separating short single-stranded nucleic acid from long single-and double-stranded nucleic acid, and associated biomolecular assays: Methods and kits are provided for detecting the presence or absence of target nucleic acid sequences in a sample. The methods and kits involve the use of negatively charged nanoparticles and the electrostatic interactions between the metal nanoparticles and nucleic acid molecules. The methods rely upon the differential interaction of...

20060166221 - Methods of diagnosing essential thrombocythemia: This invention relates to diagnosis of essential thrombocythemia. More specifically, this invention provides methods of diagnosing essential thrombocythemia by detecting a decrease in gene expression or protein levels of type 3 17β-hyrdroxysteroid dehydrogenase, and an increase in gene expression or protein levels of type 12 17β-hyrdroxysteroid dehydrogenases, in a test...

20060166255 - Methods of using 48149, a human aminopeptidase family member: Isolated nucleic acids molecules, designated 48149 nucleic acid molecules, which encode human Aminopeptidase N, are disclosed. The invention provides methods of modulating 48149 activity, which is associated with the formation of atherosclerotic lesions in blood vessels. The invention further provides methods of treating, preventing and diagnosing cardiovascular disorders such as...

20060166199 - Methods to assess quality of microarrays: The present invention relates to methods and compositions for assessing the quality of microarrays. In particular, the invention relates to the use of quality control probes that are synthesized on the microarray monomer by monomer in a step-by-step synthesis. By assessing the degree of signal from the quality control probes...

20060166243 - Methods to increase nucleotide signals by raman scattering: The methods and apparatus disclosed herein concern nucleic acid sequencing by enhanced Raman spectroscopy. In certain embodiments of the invention, nucleotides are covalently attached to Raman labels before incorporation into a nucleic acid. In other embodiments, unlabeled nucleic acids are used. Exonuclease treatment of the nucleic acid results in the...

20060166235 - Methods, compositions, and kits for forming labeled polynucleotides: The present teachings provide novel methods, compositions, and kits for forming multi-labeled polynucleotides. In some embodiments of the present teachings, multiplexed amplification reactions are performed with a plurality of primer pairs, wherein one primer in a given primer pair comprises a distinct label. Additional labeling of the resulting amplicons can...

20060166231 - Molecular indicators of breast cancer prognosis and prediction of treatment response: The present invention relates to quantitative molecular indicators that can guide clinical decisions in breast cancer, such as estrogen receptor (ESR1)-positive, lymph node-negative breast cancer. In particular, the invention concerns certain genes, the varied expression of which indicates the likelihood of recurrence of surgically resected breast cancer in patients who...

20060166203 - New sequencing method for sequencing rna molecules: The present invention provides a method for determination of the identity of at least one nucleotide in a RNA-molecule comprising the steps of: (i) providing the RNA-molecule, an oligonucleotide primer binding to a predetermined position of the RNA molecule, a reverse transcriptase, deoxynucleotides and other necessary reagents, in a reaction...

20060166213 - Novel compositions and methods in cancer: The present invention relates to novel sequences for use in detection, diagnosis and treatment of cancers. The invention provides cancer-associated (CA) polynucleotide sequences whose expression is associated with cancer. The present invention provides CA polypeptides associated with cancer and provides diagnostic compositions and methods for the detection of cancer. The...

20060166252 - Novel methods of constructing libraries of genetic packages that collectively display the members of a diverse family of peptides, polypeptides or proteins: Methods useful in constructing libraries that collectively display members of diverse families of peptides, polypeptides or proteins and the libraries produced using those methods. Methods of screening those libraries and the peptides, polypeptides or proteins identified by such screens....

20060166219 - Ntrk1 genetic markers associated with progression of alzheimer's disease: Haplotypes in the NTRK1 gene associated with progression of Alzheimer's Disease are disclosed. Compositions and methods for detecting these NTRK1 haplotypes are disclosed....

20060166222 - Nucleic acid enzyme ligation sensor: The present invention provides a calorimetric light-up sensor for determining the presence and optionally the concentration of an analyte in a sample. Methods of utilizing the sensor and kits that include the sensor also are provided. The sensor utilizes nucleic acid enzymes that ligate substrate fragments to form aggregates from...

20060166241 - Nucleic acid isolation unit and method using intercalator: Provided are nucleic acid isolation unit and method. The method includes immobilizing an aromatic compound-containing nucleic acid intercalator on a solid support; contacting a first buffer solution containing a nucleic acid sample to be purified to the intercalator immobilized on the solid support to bind the intercalator with nucleic acids...

20060166204 - Parkinson's disease susceptibility haptotype as a tool for genetic screening: Specific PON1 and ACHE alleles segregate in linkage, forming an haplotype which directly correlates with higher susceptibility to develop Parkinson's Disease (PD). This PD-susceptibility haplotype is herein presented as a tool for predicting the risk of developing Parkinson's Disease and its severity, both for an individual and for the population...

20060166261 - Pcr and hybridization methods utilizing electrostatic transportation and devices therefor: A biological sample (droplets containing DNA and primer) (9) is prepared in a chemically inert liquid layer (8), is electrostatically transported by the action of an electrostatic transportation plate. (1) with electrostatic transportation electrodes (2) and is heated at a predetermined position of the electrostatic transportation plate (1), thus carrying...

20060166262 - Pcr and hybridization methods utilizing electrostatic transportation and devices therefor: A biological sample (droplets containing DNA and primer) (9) is prepared in a chemically inert liquid layer (8), is electrostatically transported by the action of an electrostatic transportation plate (1) with electrostatic transportation electrodes (2) and is heated at a predetermined position of the electrostatic transportation plate (1), thus carrying...

20060166230 - Predicting response to chemotherapy using gene expression markers: The present invention provides gene expression information useful for predicting whether cancer patients are likely to have a beneficial response to treatment response with chemotherapy....

20060166258 - Preservation of rna in a biological sample: To preserve RNA in a biological sample for analysis, the sample is incubated with an RNA preservative capable of precipitating RNA in an aqueous solution, such as a triphenylmethane dye (e.g., methyl green, crystal violet, pararosaniline, or tris-(4-aminophenyl)methane), cresyl violet, or cobalt ions. RNA preservation may be used in an...

20060166238 - Probes, libraries and kits for analysis of mixtures of nucleic acids and methods for constructing the same: The invention relates to nucleic acid probes, nucleic acid probe libraries, and kits for detecting, classifying, or quantifying components in a complex mixture of nucleic acids, such as a transcriptome, and methods of using the same. The invention also relates to methods of identifying nucleic acid probes useful in the...

20060166218 - Protective coating for array material deposition: An array is formed with a protective cover on a substrate. The protective cover is patterned to produce an array of openings to the substrate. Desired material is deposited on the substrate through the openings. The protective cover may then be removed. In one embodiment, the protective cover is a...

20060166227 - Protein expression profiling: Disclosed are compositions and methods for detecting small quantities of analytes such as proteins and peptides. The method involves associating a primer with an analyte and subsequently using the primer to mediate rolling circle replication of a circular DNA molecule. Amplification of the DNA circle is dependent on the presence...

20060166254 - Protocol for optimizing purification of enzymatically active human telomerase: This invention provides purified telomerase and methods of purifying it. The methods involve the use of several sequential steps, including the use of matrices that bind molecules bearing negative charges, matrices that bind molecules bearing positive charges, intermediate-selectivity matrices, methods that separate molecules based on their size, shape, or buoyant...

20060166263 - Randomized libraries of zinc finger proteins: The present invention relates to methods of using libraries of randomized zinc finger proteins to identify genes associated with selected phenotypes....

20060166196 - Reporter gene assay method: The invention provides a reporter gene assay method by which the sex hormone-like activity inherent in a test substance can be accurately assayed excluding the effects caused by a decrease in cell activity (protein expressing capacity). The assay method is a reporter gene assay method using luciferase-expressing cells which comprises...

20060166245 - Rolling circle amplification of micro-rna samples: The compositions of the present invention find use in amplifying RNA obtained from subjects, particularly very small RNA samples. The methods allow conversion of RNA into circularized cDNA suitable for amplification by rolling circle replication. The amplified cDNA is then transcribed into RNA resulting in amplified RNA....

20060166211 - Ss 1,3-n-acetyl-d-galactosamine transferase protein, nucleic acid encoding the same and method of examining canceration using the same: The N-acetyl-D-galactosamine transferase protein of the present invention is characterized by transferring N-acetyl-D-galactosamine to N-acetyl-D-glucosamine with β1,3 linkage, and it preferably has the amino acid sequence shown in SEQ ID NO: 2 or 4. The canceration assay according to the present invention uses a nucleic acid for measurement which hybridizes...

20060166265 - Survivin-binding proteins, encoding nucleic acids, and methods of use: In accordance with the present invention, there are provided novel Survivin-binding-proteins (SBPs). Nucleic acid sequences encoding such proteins and assays employing same are also disclosed. The invention SBPs can be employed in a variety of ways, for example, for the production of anti-SBP antibodies thereto, in therapeutic compositions and in...

20060166253 - Tumor tissue microarrays for rapid molecular profiling: An array-based technology facilitates rapid correlated gene copy number and expression profiling of a very large number of human tumors. Hundreds of cylindrical tissue biopsies (diameter 0.6 mm) from morphologically representative regions of individual tumors can be arrayed in a single paraffin block. Consecutive sections from such arrays provide targets...

20060166251 - Use of sfrps as markers of bmp activity: The disclosure provides assay systems for evaluating the presence of bone morphogenetic protein (BMP) activity in a cell by evaluating the gene expression of secreted Frizzled Related Protein 2 and 3 (sFRP2 and sFRP3). The sFRP2 and sFRP3 gene expression may be detected at the RNA or protein levels. The...

20060166260 - Visualization of avian eggs: The invention includes methods of visualizing and manipulating avian eggs employing TPLSM....

20060166275 - Amyloid beta protein (globular assembly and uses thereof): The invention described in this disclosure involves a new composition of matter, amyloid beta-derived dementing ligands (ADDL's). ADDLs consist of amyloid β peptide assembled into soluble globular non-fibrillar oligomeric structures that are capable of activating specific cellular processes. The invention further encompasses methods for assaying the formation, presence, receptor protein...

20060166270 - Detection of demyelination: The present invention relates to a method for the detection of demyelinization in a mammal, comprising providing an NMR spectrum of metabolites in a body fluid of an individual of said mammal in which demyelinization is suspected and comparing said NMR spectrum with a difference profile, comprising a plurality of...

20060166268 - Diagnosis of glaucoma by complex autoantibody repertoires in body fluids: The invention relates to a method for the diagnosis of glaucoma based on the composition of autoantibodies against ocular antigens in body fluids of individuals. The method is characterized in that in a first step, autoantibodies against retinal and/or optic nerve head antigens are detected and measured in body fluids...

20060166273 - Enhanced methods for crystallographic structure determination employing hydrogen exchange analysis: The present invention provides methods for crystallographic structure determination employing hydrogen exchange analysis. Hydrogen exchange analysis is used to identify unstructured regions of a protein, and then hydrogen exchange analysis repeated after the protein is admixed with candidate agents and/or conditions that may induce structure in said identified unstructured regions....

20060166274 - G protein coupled receptor (gpcr) agonists and antagonists and methods of activating and inhibiting gpcr using the same: The invention relates generally to G protein coupled receptors and in particular to agonists and antagonists of G protein receptors and methods of using the same....

20060166280 - Labor biomarkers, methods comprising same, and methods targeting same: The present invention provides methods of predicting or detecting labor in a female subject and methods of testing a compound for an ability to delay the onset of labor. The present invention also provides methods of testing a labor marker useful in the diagnostic methods, isolated peptides identified in the...

20060166267 - Method for extracting proteins: The present invention relates to a method and a kit for the sequential extraction of proteins for the production of partial proteomes from a complete proteome. In particular, the method according to the invention, besides other partial proteomes, gives a partial proteome of proteins from the cell nucleus interior separated...

20060166278 - Method for synthesizing sugar chain(s): An object of the present invention is to provide a method for efficiently chemically synthesizing biomolecules including a nucleotide (nucleic acid), a peptide (protein), or a sugar chain, as representative examples. The present invention provides a method of solid-phase synthesis of sugar chain(s) for synthesizing multiple types of sugar chains...

20060166272 - Method of adjudicating on acute aortic dissection and reagent for adjudication: A method of evaluating acute aortic dissection, which comprises measuring a D-dimer concentration in blood separated from a human, and determining whether or not acute aortic dissection (Stanford type A or Stanford type B) has developed, on the, basis of the measured concentration, a reagent for evaluating performing the evaluation...

20060166276 - Method of diagnosis and agents useful for same: The present invention relates generally to a method of diagnosing, predicting or monitoring the development or progress of heart failure in a mammal and, more particularly, to a method of diagnosing, predicting or monitoring the development or progress of congestive heart failure in a mammal. The present invention contemplates a...

20060166269 - Method of measuring binding site on plasma protein of plasma protein-binding drug and method of measuring plasma protein mutation: The present invention relates to a method for readily determining the binding site on plasma protein and binding displacement effect of a drug having binding affinity to plasma protein, and application of the method to clinical diagnosis. That is to say, the present invention provides a method for determining the...

20060166271 - Non-radioactive schilling test: The present invention relates to a new method named the COBASORB test, which can be used for testing the cause of cobalamin malabsorption in humans. The COBASORB test contains three separate tests (first, second and third test) that can be performed separately, sequentially or in random order and number. The...

20060166277 - Nucleic acids and polypeptides useful for diagnosing and treating complications of pregnancy: Disclosed herein are methods for diagnosing or treating pregnancy related hypertensive disorders that include the use of a polypeptide or a nucleic acid encoding a polypeptide selected from the following: follistatin related protein, interleukin 8, inhibin A, VEGF-C, angiogenin, beta fertilin, hypothetical protein, leukocyte associated Ig-like receptor secreted protein, erythroid...

20060166266 - Screening method for regulatory enzymes employing tribrid (tri-hybrid) system: A method for screening regulatory enzymes is described. The method involves the use of a tribrid cell engineered to express a DNA library....

20060166279 - Sea-trosy and related methods: A method for preferentially observing an exposed position (1c) of a macromolecule. A sample is obtained having a macromolecule (1a) with a first proton (1) and a second molecule (2a) with a second proton (2); then applying a magnetic field (4) to the sample and irradiating the sample with a...

20060166283 - Means for detecting neurodegenerative processes and applications of same: The invention relates to novel markers of the neurodegenerative process, comprising the α chain of the modified ATP synthase. The invention also relates to methods of detection, diagnosis, antibodies, kits and applications of same....

20060166282 - Methods and compositions for screening cell binding molecules: Ligands, such as antibodies, are screened for binding to cell surface moieties such as receptors. Multiple cell types and/or binding ligands can be screened simultaneously in multiplexed assays. The screening assays employ membrane-anchored electrophoretic probes for detection of binding of the ligand to the cell surface....

20060166281 - Potent, selective and non toxic c-kit inhibitors: The present invention relates to a screening method allowing the identification and selection of compounds targeting the transphosphorylase (also called phosphotransferase) domain of c-kit, more particularly compounds selected to be potent inhibitors of constitutively activated c-kit, while being unable to inhibit other activation pathways as for example the pathways leading...

20060166294 - Apoptosis marker antibodies and methods of use: Disclosed are antibodies that specifically recognize the new amino terminus of a protein cleaved by a protease during apoptosis. Methods of using and making the antibodies are also provided. The antibodies are particularly useful in methods of detecting apoptosis and testing candidate compounds for enhancing or inhibiting apoptosis....

20060166288 - Assay for the screening of compounds acting through erbb-2: The invention provides a cellular proliferation assay for a compound acting through erbB-2 which comprises: i) a cell comprising erbB-2 and erbB-3 and said cell is responsive to ligand stimulated cell proliferation under conditions suitable for cell proliferation; ii) a first ligand which is a ligand for erbB-3 capable of...

20060166291 - Kid31 and antibodies that bind thereto: The invention provides the identification and characterization of disease and cancer-associated antigen, KID31. The invention also provides a family of monoclonal antibodies that bind to antigen KID31, methods of diagnosing and treating various human cancers and diseases that express KID31....

20060166289 - Method for analyzing tumor agressivity comprising measurement of polymerized actin: The invention concerns a method for analyzing tumor agressivity of cancer cells comprising measurement of the amount of stationary polymerized actin in a lysate of said cells. Advantageously, the measurement of the amount of stationary actin is carried out by fluorescence static polarization....

20060166287 - Method for identifying compounds for suppressing metastasizing tumor cells: The present invention relates to a method for identifying compounds which suppress and/or delay the development of invasive or metastasizing tumor cells of the colon, of the pancreas, of the ovaries or of the thyroid....

20060166292 - Methods of diagnosing and treating prostate cancer: The invention provides for novel diagnostic methods for detecting prostate cancer. The invention also provides methods for determining the effectiveness of prostate cancer treatment. These methods take advantage of the finding that a cellular protein termed Beta Protein 1 (BP1) is expressed at elevated levels in prostate cancer tissue and...

20060166290 - Primary central nervous system tumor specific behab isoforms: The present invention comprises compositions and methods related to a glycosylation-variant BEHAB polypeptide, a poly-sialyated BEHAB polypeptide, and methods of use thereof....

20060166293 - Zinc alpha-2-glycoprotein as indicator of cancer: The present invention relates, in general, to methods of diagnosing and monitoring cancer and inflammatory diseases/disorders and, in particular, to methods of diagnosing and monitoring cancer and inflammatory diseases/disorders that comprise assaying for elevated levels of zinc alpha-2-glycoprotein (ZAG) in serum and other body fluids. The invention also relates to...

20060166285 - Charged permeation layers for use on active electronic matrix devices: An electronic device including at least one electrode on a substrate and a permeation layer overlaying the at least one electrode. The permeation layer comprises a polymer having a plurality of negatively-charged moieties. The negatively charged moieties may be carboxylates. In one embodiment, the negatively charged moieties are carboxylates from...

20060166286 - Methods and compositions for tagging via azido substrates: The invention provides methods and compositions for azide tagging of biomolecules. In one embodiment of the invention, proteins are tagged by metabolic incorporation of prenylated azido-analog substrates. Examples of such analogs are azido farnesyl diphosphate and azido farnesyl alcohol. The azido moiety in the resulting modified proteins provides an affinity...

20060166284 - Novel tissue factor targeted antibodies as anticoagulants: This invention relates to novel antibodies that bind with greater affinity to the factor VIIa/tissue factor (FVIIa/TF) complex than to tissue factor (TF) alone, do not compete for binding to TF with FVII and FX, an inhibit FX activation. The antibodies bind at the site of injury and prevent the...

20060166297 - High affinity anti-n¹,n¹²-diacetylspermine monoclonal antibody: The present invention provides an anti-diacetylspermine specific monoclonal antibody which satisfies a specific measuring condition in an immunoreaction system between a solid-phased diacetylspermine and the anti-diacetylspermine specific monoclonal antibody, wherein the immunoreaction system comprises a sample diacetylspermine or sample N1-acetylspermidine to inhibit the immunoreaction; and the specific measuring condition is...

20060166295 - Method for determination of likelihood of occurrence of preterm labor in pregnant females: The presence of 3-chlorotyrosine in vaginal secretions in a predictor of the likelihood preterm premature rupture of the fetal membranes, i.e., the chorioamnion and/or the occurrence of the risk of preterm labor. 3-chlorotyrosine is a marker for the excessive production of hypochlorous acid which causes focal areas of increased collagen...

20060166296 - Standard compound for immunoassay for dioxin and method of immunoassay for dioxin: e

20060166298 - Assay system for simultaneous detection and measurement of multiple modified cellular proteins: A method and kit for simultaneous detection and/or determination of a plurality of modified proteins in a sample. The method comprises: a) contacting the sample under mild protein denaturation conditions with a plurality of first antibodies capable of binding to a specific target protein, the first antibodies being immobilized on...

20060166299 - Proteins in type 2 diabetes: The present invention relates to the use of naturally occurring compounds and derivatives thereof as markers for predisposition of diabetes related diseases. The invention also relates to a pharmaceutical composition for treatment of the diabetes related diseases....

20060166300 - Method of measuring homocysteine: A method for detecting or measuring homocysteine in a sample includes the steps of (a) reacting a D-amino acid present in a sample with a D-amino acid converting enzyme to convert the D-amino acid into a substance that does not serve as a substrate of D-amino acid oxidase or D-amino...

20060166301 - Stable chromogenic test reagent and its use in coagulation-diagnostic tests: The present invention relates to a chromogenic test reagent which comprises a chromogenic peptide substrate and an inhibitor of fibrin polymerization, which is particularly suitable for being used in coagulation-diagnostic tests and which is distinguished by the fact that it exhibits a nigh degree of stability and/or a long shelf...

20060166302 - Handheld raman blood analyzer: Methods and apparatus for in vitro detection of an analyte in a blood sample using low resolution Raman spectroscopy are disclosed. The blood analyzer includes a disposable strip for receiving a sample of blood on a target region, the target region including gold sol-gel to provide surface enhanced Raman scattering....

20060166304 - Polynucleotides encoding insect 3-ketosphinganine reductase and uses thereof: The instant invention provides nucleic acids encoding insect 3-ketosphinganine reductase, as well as insect 3-ketosphinganine reductase encoded thereby. The invention further provides methods of identifying agents that modulate a level of 3-ketosphinganine reductase mRNA, polypeptide, or enzyme activity. Such agents are candidate insecticidal compounds....

20060166303 - Use of cardiac hormones for assessing a cardiovascular risk with respect to the administration of anti-inflammatory drugs: The present invention relates to the use of cardiac hormones, particularly natriuretic peptides, for diagnosing the cardiovascular risk of a patient who is a candidate for administration of a selective Cox-2 inhibitor, comprising the steps of a) measuring, preferably in vitro, the level of a cardiac hormone, b) diagnosing the...

20060166305 - Animal cell confluence detection method and apparatus: The invention provides an apparatus and process for detecting the degree of confluence of animal cells being cultured in a well plate. A well plate is arranged in an imaging station and illuminated with a ring of LEDs, or other optical source, from below at an oblique angle. An image...

20060166306 - Methods and compositions for in vitro synthesis of biological macromolecules in a cell-free system enriched with atp-sulfurylase: The present invention provides methods and composition for enhancing in vitro synthesis of biological macro-molecules in a call-free system where ATP is required as a primary energy source, wherein said cell-free system is enriched with ATP-sulfurylase The invention relates also to as cell-free systems and cell-free extracts enriched with ATP-sulfurylase...

20060166307 - Mixing devices for chemical lysis of cells: The invention relates to flow-through devices for mixing fluids and uses thereof for mixing and for lysing cells to release biological compounds of interest. The invention particularly encompasses flow-through methods for mixing and chemical methods for the isolation and purification of plasmid DNA from cell culture....

20060166308 - Stereoisomerically enriched beta-lactams using candida antarctica: The present disclosure provides enzymatic methods for generating stereoisomerically pure products by resolving a racemic mixture of N-protected β-lactams with a lipase from Candida antarctica with high stereospecificity. The presence of a carbamate protecting group, such as the tert-butoxycarbonyl group protecting group, on the β-lactam enhances enzyme catalysis and stereoselectivity....

20060166326 - 32142, 21481, 25964, 21686, novel dehydrogenase molecules and uses therefor: The invention provides isolated nucleic acids molecules, designated DHDR nucleic acid molecules, which encode novel DHDR-related dehydrogenase molecules. The invention also provides antisense nucleic acid molecules, recombinant expression vectors containing DHDR nucleic acid molecules, host cells into which the expression vectors have been introduced, and nonhuman transgenic animals in which...

20060166319 - Charging trna with pyrrolysine: Provided herein is a method for preparing a cell that, when exposed to the non-canonical amino acid, pyrrolysine or a derivative thereof and transformed or transfected with a polynucleotide comprising an in-frame UAG or TAG codon incorporates the pyrrolysine residue or derivative thereof into the protein or polynucleotide into the...

20060166323 - Compositions comprising promoter sequences and methods of use: Nucleic acid molecules, fragments and variants thereof having promoter activity are provided in the current invention. The invention also provides vectors containing a nucleic acid molecule of the invention and cells comprising the vectors. Methods for making and using the nucleic acid molecules of the invention are further provided....

20060166322 - Egvi endoglucanase and nucleic acids encoding the same: The present invention provides a novel endoglucanase nucleic acid sequence, designated egl6, and the corresponding EGVI amino acid sequence. The invention also provides expression vectors and host cells comprising a nucleic acid sequence encoding EGVI, recombinant EGVI proteins and methods for producing the same....

20060166325 - Glial cell line-derived neurotrophic factor receptor: The present invention relates to glial cell line-derived neurotrophic factor (GDNF), a potent neurotrophin that exhibits a broad spectrum of biological activities on a variety of cell types from both the central and peripheral nervous systems. The present invention involves the cloning and characterization of a high affinity receptor for...

20060166327 - Human g-protein receptor hgber32: Human G-protein coupled receptor polypeptides and DNA (RNA) encoding such polypeptides and a procedure for producing such polypeptides by recombinant techniques is disclosed. Also disclosed were methods for utilizing such polypeptides for identifying antagonists and agonists to such polypeptides and methods of using the agonists and antagonists therapeutically to treat...

20060166320 - In vitro expression of the hcv arfp/f-core‘coding open reading frame: A method of producing and purifying an HCV polypeptide, wherein the method comprises providing host cells containing an expression vector comprising a polynucleotide operably linked to expression control elements, wherein the polynucleotide is an open reading frame that overlaps the core gene in the +1 frame (core+1 ORF) of HCV,...

20060166314 - Method for rational mutagenesis of alpha/beta t-cell receptors and correspondingly mutated mdm2-protein specific alpha/beta t-cell ceceptores: The invention relates to the rational mutagenesis of polypeptides of α/β T-cell receptors that mediate an oncogen-specific T-cell response, nucleic acids encoding these and their use in the therapy, diagnosis and/or prevention of cancerous diseases. The invention further relates to a T-cell response-mediating MDM2-protein-specific α/β T-cell receptor, which has been...

20060166318 - Methods, compositions, and cells for encapsidating recombinant vectors in aav particles: Isolated recombinant polynucleotides comprising elements which promote encapsidation into AAV particles, packaging cells comprising the recombinant polynucleotides, and methods for their use are provided in the present invention. These isolated recombinant polynucleotides comprise a non-AAV ITR encapsidation element (such as the P1 sequence located within the AAV S1 integration site...

20060166309 - Novel g-protein coupled receptors: The invention provides isolated nucleic acid and amino acid sequences of four novel G-protein coupled receptors that are amplified in breast cancer cells, antibodies to such receptors, methods of detecting such nucleic acids and receptors, and methods of screening for modulators of G-protein coupled receptors....

20060166313 - Novel glycero kinase, gene thereof and process for producing the glycerol kinase by using the gene: Relating to a gene encoding a new glycerol kinase and a method for preparing the enzyme by gene recombination technique. A Glycerol kinase which has high resistance against preservative, a recombinant vector comprising a gene encoding the glycerol kinase, a transformant prepared by transforming a host cell with the recombinant...

20060166312 - Novel microorgnaism sensitive to lysozyme: A microorganism of the genus Rhodococcus is provided which has a higher sensitivity to lysozyme at a low concentration than a wild-type strain, which can easily cause cell lysis, and from which a recombinant protein expressed therein is easily recovered. More specifically, a mutant microorganism of the genus Rhodococcus having...

20060166311 - Novel notch-origin polypeptides and biomarkers and reagents using the same: It is intended to provide extracellular markers whereby Notch signal transduction can be detected. Polypeptides (Nβ), which are novel peptides originating in Notch protein and released form cells in the step of the nuclear migration of NICH (Notch intracellular cytoplasmic domain) due to the extracellular digestion and the subsequent protein...

20060166317 - Nucleic acids encoding sialytransferases from c. jejuni: This invention provides prokaryotic glycosyltransferases, including a bifunctional sialyltransferase that has both an α2,3- and an α2,8-activity. A β1,4-GalNAc transferase and a β1,3-galactosyltransferase are also provided by the invention, as are other glycosyltransferases and enzymes involved in synthesis of lipooligosaccharide (LOS). The glycosyltransferases can be obtained from, for example, Campylobacter...

20060166316 - Phosphodiesterase 10a7 isoforms and methods of use: The invention relates to mammalian phosphodiesterase PDE10A7 isoforms, as well as the nucleic acids encoding such polypeptides. The invention further relates to methods for utilizing such polypeptides in diagnostic assays and in methods of screening potential modulators, especially inhibitors, of the novel PDE10A7 disclosed herein. The polypeptides of the invention...

20060166315 - Process for producing pnpase: The invention provides a process for producing PNPase, wherein PNPase can be produced easily with high efficiency, and problematic contamination with endotoxin in synthesis of a nucleic acid polymer as a raw material of pharmaceutical preparations can be reduced. PNPase is produced by Escherichia coil or the like having a...

20060166321 - Recombinant influenza vectors with tandem transcription units: The invention provides a composition useful to prepare influenza viruses, e.g., in the absence of helper virus, using vectors which include tandem transcription cassettes containing PolI and/or PolII promoters....

20060166324 - Screening tools for discovery of novel anabolic agents: The disclosure provides nucleic acids including the polynucleotide sequence of the human MAFbx core promoter involved in muscle specific expression. Also provided are reporters operably linked to a polynucleotide sequence including MAFbx transcription regulatory sequences, and constructs including polynucleotides that encode reporters and other polynucleotide sequences operably linked to the...

20060166310 - Serotonin receptor: This invention relates to a protein, termed INPIONCH1, herein identified as a 5-HT3 receptor and to the use of this protein and nucleic acid sequence from the encoding gene in the diagnosis, prevention and treatment of disease....

20060166328 - Igf-i fusion polypeptides and therapeutic uses thereof: A fusion protein comprising at least one IGF1 variant component and a fusion component (F), and, optionally, a signal sequence, exhibiting improved stability relative to the native IGF1 or IGF2 polypeptide. The fusion component (F) may be a multimerizing component, a targeting ligand, or another active or therapeutic compound. IGF1...

20060166329 - Albumin fusion proteins: The present invention encompasses albumin fusion proteins. Nucleic acid molecules encoding the albumin fusion proteins of the invention are also encompassed by the invention, as are vectors containing these nucleic acids, host cells transformed with these nucleic acids vectors, and methods of making the albumin fusion proteins of the invention...

20060166330 - Process for producing purified anthocyanin and crystalline anthocyanin: Provided are a process for producing purified anthocyanidin glucoside in which a rhamnose end of anthocyanidin rutinoside is cleaved using rhamnosidase to convert the anthocyanidin rutinoside component into anthocyanidin glucoside, the anthocyanidin glucoside component being then purified and isolated; or a crystalline anthocyanidin glucoside salt obtained by further crystallizing the...

20060166331 - Apparatus and method for preparative scale purification of nucleic acids: Apparatus and methods are described for pharmaceutical grade manufacture extrachromosomal nucleic acids from cell lysates using flotation to separate and eliminate undesired insoluble cellular debris including chromosomal DNA from the lysates. A gas is introduced to controllably generate bubbles that reduce the density of the cell debris and create a...

20060166332 - Method for ligating nucleic acids and molecular cloning: The invention provides cells and methods of circularizing linear DNA molecules. The cell is an isolated Escherichia coli cell which transiently expresses the Cre recombinase protein from an integrated Cre recombinase gene, and which is at least transiently repressed for RecBCD activity. The cells are used in a method of...

20060166334 - Method and compositions for rapidly modifying clones: A method for removing extraneous nucleotides in a cloned coding sequence using a type IIs endonuclease, the method comprising introducing a linker that comprises at least one recognition site for a Type IIs restriction endonuclease to the ORF, cloning the ORF into a suitable vector, and removing the extraneous nucleotides...

20060166333 - Methods of collecting and transporting vaginal discharge for detection of infectious organisms and to facilitate cervical cancer screening: The present invention provides a method of testing for the presence of infectious disease agents or host genetic markers comprising applying a device comprising an absorbent and porous material onto the introitus of a female patient; encouraging air drying of at least a portion of the collected vaginal discharge while...

20060166335 - Glucuronofucan sulfate: Three enzymes degrading sulfated glucuronofucan; processes for producing these enzymes; activators for these enzymes; glucuronofucan sulfate and degradation products thereof; and a novel microorganism....

20060166336 - Modified alternan: Four new Penicillium spp. isolates (NRRL 21966, NRRL 21967, NRRL 21968, and NRRL 21969) are capable of essentially quantitative conversion of native alternan to a polymeric modified form having a lower apparent molecular weight than native alternan. A fifth isolate (NRRL 30489) obtained from a survey of deposited organisms and...

20060166338 - Alleles of the mqo gene from coryneform bacteria: The invention relates to mutants and alleles of the coryneform bacterium mqo gene which encodes malate quinone oxidoreductases which contain any amino acid apart from L-serine at position 111, or a comparable position, in the amino acid sequence, and to processes for fermentatively preparing amino acids, preferably L-lysine, L-tryptophan and...

20060166337 - Process for producing optically active erythro 3-cyclohexylserines: (wherein, R1 means an alkanoyl group having 1-10 carbons, a benzoyl group, a halogen-substituted alkanoyl group having 1-5 carbons or a halogen-substituted benzoyl group, and R2 means a phenyl group or a cyclohexyl group) is subjected to asymmetrical hydrolysis with L-aminoacylaseorD-aminoacylase, there by giving unreacted N-acyl-D-erythro3-substituted serine in the case...

20060166340 - Microorganism and the process for preparation of pravastatin sodium: The invention provides a novel microorganism producing pravastatin sodium, as well as the method for producing pravastatin sodium by using this microorganism. Micropolyspora roseoalba CGMCC 0624 of the invention is highly tolerant to mevastatin sodium, and has a high transformation rate of mevastatin sodium, and can produce pravastatin sodium with...

20060166339 - Production of l-aldonolactone: The present invention provides a process for the production of L-aldonolactone from L-aldohexose, especially for producing L-gulono-1,4-lactone or L-gulonic acid from L-gulose and producing L-galactono-1,4-lactone or L-galactonic acid from L-galactose by a microorganism belonging to the genus Pseudomonas or the genus Gluconobacter....

20060166341 - Method for reproducing pleuromutilins: Invented are non-peptide TPO mimetics. Also invented is a method of treating thrombocytopenia, in a mammal, including a human, in need thereof which comprises administering to such mammal an effective amount of a selected hydroxy-1-azobenzene derivative....

20060166342 - Process for producing optically active 3-hydroxypropionic ester derivative: The present invention is to provide a process for simply producing an optically active 3-hydroxypropionic ester derivative useful as a medicament intermediate from an inexpensive material. More specifically, the present invention is directed to a process for producing an optically active 3-hydroxypropionic ester derivative comprising reacting an acetic ester derivative...

20060166343 - Photosynthetic hydrogen production: A process for the production of hydrogen, comprising the steps of: (i) providing a photosynthetic microorganism having electron transfer capability through a photosynthetic “light” reaction pathway and through a respiratory electron transfer chain involving an oxidative phosphorylation pathway, and which expresses a hydrogenase, wherein regulation of the oxidative phosphorylation pathway...

20060166344 - Bacterial outer memberane vesicles: Existing methods of meningococcal OMV preparation involve the use of detergent during disruption of the bacterial membrane. According to the invention, membrane disruption is performed substantially in the absence of detergent. The resulting OMVs which retain important bacterial immunogenic components, particularly (i) the protective NspA surface protein, (ii) protein NMB2132...

20060166345 - Method of inactivating enveloped viruses in a viral preparation of non-enveloped viruses: The present invention relates to a method of inactivating enveloped viruses in a viral preparation predominantly containing non-enveloped viruses by the action of a solvent at a temperature of between −5° C. and +50° C. and at a pH of between about 5 and 9. Its subject is also a...

20060166346 - Support for holding a complexed enrichment of degrading bacteria and manufacturing method thereof, novel bacteria, and method of cleaning pollution environment and device thereof: The degrading bacteria A may be a PCNB-degrading bacteria, particularly degrading bacteria containing degrading bacteria having part or all of the bacteriological characteristics of Nocardioides sp. PD653 and the degrading bacteria B may be degrading bacteria containing degrading bacteria having part or all of the bacteriological characteristics of β-Proteobacteria CDB21....

20060166347 - Sample preparation devices and methods: A method and device for preparing biological samples includes lysing the microorganisms, and collecting a desired biological material....

20060166348 - Anaerobic biological degradation of hydrocarbons: The invention relates to a method for the anaerobic biological degradation of aromatic hydrocarbons (in particular benzene), and to a specific mixture and the use thereof for this degradation. According to the invention, the anaerobic biological degradation of aromatic hydrocarbons present at a contaminated location is stimulated and stabilized by...

20060166349 - Recovery of plasmids in an aqueous two-phase system: The present invention is a method for the purification of plasmid DNA comprising to provide a composition comprising a first polymer having inverse solubility characteristics and a second polymer immiscible in the first polymer; contacting said solution with an aqueous solution comprising plasmid DNA; providing phase separation and isolating the...

20060166351 - Cell capturing apparatus and method of capturing cell: A path defines an opening smaller than a cell in a cell capturing apparatus. A negative pressure generating device is connected to the path so as to generate negative pressure within the path. A negative pressure controlling device is connected to the path so as to adjust the negative pressure...

20060166352 - Device and method for analyte detection: The invention is directed to a method and device for simultaneously testing a sample for the presence, absence, and/or amounts of one or more of a plurality of selected analytes. The invention includes, in one aspect, a device for detecting or quantitating a plurality of different analytes in a liquid...

20060166350 - Method of detecting an analyte in a fluid: A method for the detection of an analyte in a fluid, comprising contacting the fluid with a holographic element comprising a medium and a hologram disposed throughout the volume of the medium, wherein an optical characteristic of the element changes as a result of a variation of a physical property...

20060166353 - Process for realishing a biomorphic, stereolithographed phantom, which is multicompartmental and suitable for multanalytical examinations, and relevant device: A process for preparing digital images for realising a biomorphic multicompartmental phantom, includes a phase A.1 of acquisition of images of the organ belonging to the analysed living being, forming a volumetric image defined by voxels, a phase A.2 of identification of tissues and/or tissue liquids and a phase B...

20060166354 - Bioreactors with multiple chambers: A bioreactor for cultivating living cells in a liquid medium. In one embodiment of the present invention, the bioreactor has a first substrate having a first surface and an opposite second surface, defining a chamber therebetween for receiving the cells and the liquid medium. The bioreactor further has a barrier...

20060166355 - Imaging fluorescence signals using telecentric optics: The present invention relates to the field of DNA analysis. In particular, the present invention is directed to a device for the parallel imaging of fluorescence intensities at a plurality of sites as a measure for DNA hybridization. More particular, the present invention is directed to a device to image...

20060166358 - Biofilm incubation: Biofilm forming organisms are incubated to form a biofilm on projections by providing a flow of liquid growth medium across projections, the direction of the flow of liquid being repeatedly changed, and an assay made of the resulting biofilm. Biofilm forming organisms are incubated to form a biofilm on projections...

20060166356 - Cell culture device: The invention concerns a device designed in particular for cell culture (10) comprising a chamber (100) wherein are arranged the cells (10), said chamber (100) comprising at least one first conduit (131, 141, 151, 161, 133, 143, 153, 163) for injecting a first type of fluid (M1), at least a...

20060166357 - Integrated microfludic control employing programmable tactile actuators: Microfluidic devices having active features such as valves, peristaltic pumps, and mixing portions are fabricated to have a thin elastomeric membrane over the active features. The active features are activated by a tactile actuator external to the membrane, for example, a commercial Braille display. The display may be computer controlled,...

20060166359 - Transgenic mice containing lumulus clotting factor protease-like gene disruptions: The present invention relates to transgenic animals, as well as compositions and methods relating to the characterization of gene function. Specifically, the present invention provides transgenic mice comprising mutations in a limulus clotting factor protease-like gene. Such transgenic mice are useful as models for disease and for identifying agents that...

20060166360 - Compositions, solutions, and methods used for transplantation: This invention discloses a method for reducing the intracellular lipid storage material of a cell, tissue, or organ for transplantation and features solutions, methods and kits that induce the metabolic elimination of lipid storage in a cell, tissue, or organ. In one exemplary approach, the process involves contacting a cell,...

20060166361 - Postpartum cells derived from placental tissue, and methods of making, culturing, and using the same: Cells derived from postpartum placenta and methods for their isolation are provided by the invention. The invention further provides cultures and compositions of the placenta-derived cells. The placenta-derived cells of the invention have a plethora of uses, including but not limited to research, diagnostic, and therapeutic applications....

20060166362 - Method of inducing differentiation of bone marrow stromal cells to neural cells or skeletal muscle cells by introduction of notch gene: There is provided a method of inducing differentiation of bone marrow stromal cells to neural cells or skeletal muscle cells by introduction of a Notch gene. Specifically, the invention provides a method of inducing differentiation of bone marrow stromal cells to neural cells or skeletal muscle cells in vitro, which...

20060166363 - Modified baculovirus expression system for production of pseudotyped raav vector: The invention provides modifications to a baculovirus-based recombinant adeno associated virus (AAV) system including enhancement of the helper virus stability and construction of novel baculovirus vectors for rAAV pseudotyping. The modified system extends the flexibility of rAAV vector production and promotes the utility of AAV as, a clinically applicable gene...

20060166364 - Use of flexible bag containers for viral production: The present invention relates generally to the fields of cell banking and viral production. More particularly, it concerns a method of virus production from host cells using flexible containers....

20060160061 - Dehydration procedures for induction of cryptobiosis in insect larvae: This invention provides a method for effectively producing dehydrated larvae for educational materials without disrupting the environment. The cryptobiotic larvae for educational materials can be obtained by dehydrating larvae while gradually reducing humidity in 3 separate stages....

20060160062 - Perfusion and/or preservation solution for organs: The present invention relates to a solution for preservation, perfusion, and/or reperfusion of an organ, especially the heart, for transplantation. The solution contains peptide inhibitor(s) of protein kinase C βII (PKC βII) and/or of protein kinase C ζ (PKC ζ) and/or peptide activator(s) of protein kinase C δ (PKCδ). Methods...

20060160066 - Cellular microarrays for screening differentiation factors: Provided is a microarray platform for the culture of cells atop combinatorial matrix mixtures; enabling the study of differentiation in response to a multitude of microenvironments in parallel....

20060160064 - Devices and methods for removing target agents from a sample: The invention provides devices, test kits and methods for removing target agents from a sample. The device contains one or more porous matrices having pore sizes larger than 10 μm, and a plurality of particles impregnated therein. The target agents attach the device and are removed from the sample....

20060160065 - Method for classifying plant embryos using raman spectroscopy: A three-step method for classifying plant embryo quality using Raman spectroscopy is provided. First, a classification model is developed based on Raman spectral data of reference samples of plant embryos or any portions of plant embryos of known embryo quality. The embryo quality may be known based on a comparison...

20060160063 - Method of screening physilogically active substance: The present invention relates to a method of screening a physiologically active substance, comprising the steps of (1) contacting a transformed yeast with a test sample, wherein the transformed yeast is capable of expressing a heterogeneous protein, and shows a change in a growing state in an expression state of...

20060160070 - Association-based epitome design: Systems that facilitate immunogen design are described herein. An optimization component is provided to determine an immunogen according to at least one criterion. The immunogen comprises a set of overlapping sequences comprising sequences that are known to be and/or are likely to be immunogenic. At least one of the sequences...

20060160071 - Association-based predictions: A system comprising a machine learning classifier trained on a plurality of associations between a host and a pathogen to predict a pathogen characteristic is described herein. The pathogen characteristic can relate to a disease state of the host. Computer-executable instructions for performing a method of forecasting a portion of...

20060160067 - Chimeric gb virus b (gbv-b): The present invention relates generally to the fields of biochemistry, molecular biology, and virology. More particularly, it relates to the identification of GB virus B (GBV-B)/HCV chimeras. The invention involves nucleic acid constructs and compositions encoding GBV-B/HCV chimera, including at least part of a 5′ NTR derived from a HCV...

20060160068 - Competitive immunoassay: The present invention provides ligand-detection reagents, ligand analogs and methods for determining the presence of a ligand in a sample. The ligand-detection reagent comprises a ligand-binding antibody and a ligand analog to form an antibody-ligand analog complex wherein the ligand analog is covalently bonded to a reporter molecule. This complex...

20060160073 - Efficient algorithm for pcr testing of blood samples: Systems, processes, and devices are provided which are useful for testing blood or plasma donations to detect those specific donations which are contaminated by a virus above a predetermined level. An apparatus and process is described which forms individual, separately sealed and connected sample containers from a flexible hollow tubing...

20060160072 - Method for molecular cloning and polynucleotide synthesis using vaccinia dna topoisomerase: This invention provides a modified vaccinia topoisomerase enzyme containing an affinity tag which is capable of facilitating purification of protein-DNA complexes away from unbound DNA. This invention further provides a modified seauence specific topoisomerase enzyme. This invention provides a method of ligating duplex DNAs, a method of molecular cloning of...

20060160069 - Methods and compositions for the diagnosis of cancer: In one embodiment, the invention relates to a method of detecting cervical cancer, and other types of cancer, using a combination of at least three genomic clones, or fragments thereof, of high risk Human Papilloma Virus. For example, the invention relates to a composition comprising at least three full length...

20060160126 - 90 human secreted proteins: The present invention relates to novel human secreted proteins and isolated nucleic acids containing the coding regions of the genes encoding such proteins. Also provided are vectors, host cells, antibodies, and recombinant methods for producing human secreted proteins. The invention further relates to diagnostic and therapeutic methods useful for diagnosing...

20060160115 - Activation method of protein derived from extremely thermophilic bacterium in nucleic acid amplification reaction and use thereof: The present invention is to provide a method which makes it possible to activate RecA, and maintain its biological function in all the PCR cycles. Also, the invention is to provide a nucleic acid amplification method, and a kit for amplifying a nucleic acid which makes it possible to suppress...

20060160092 - Adip protein and use thereof: The objective of the present invention is to provide a gene encoding novel afadin DIL domain-binding protein (ADIP) and use of the novel ADIP protein. The present inventors successfully identified a novel afadin-binding protein (ADIP) using yeast two-hybrid screening in order to identify how the nectin and afadin system organize...

20060160097 - Amplification reaction vessel, and method of manufacturing the same: A nucleic acid amplification reaction vessel has a substrate, a cavity formed in the substrate, a cover plate for sealing the cavity, and a sample-injection inlet formed in the cover plate. The cavity includes a columnar structure connected to the cover plate or the substrate. The nucleic acid amplification reaction...

20060160080 - Avian sex determination method: A method for the determination of the sex of an avian subject is provided which comprises analysis of a sample from said subject with a nucleic acid probe comprising an at least 6 base pair fragment from a Female Associated Factor (FAF) nucleic acid sequence, or with an antibody to...

20060160090 - Composition splice variants and methods relating to cancer specific genes and proteins: The present invention relates to newly identified nucleic acid molecules and polypeptides present in normal and neoplastic cells, including fragments, variants and derivatives of the nucleic acids and polypeptides. The present invention also relates to antibodies to the polypeptides of the invention, as well as agonists and antagonists of the...

20060160125 - Compositions and methods for nonenzymatic ligation of oligonucleotides and detection of genetic polymorphisms: The invention is directed to novel compositions and methods for nonenzymatic ligation of oligonucleotides. In one aspect of the invention, the nonenzymatic ligation is selenium-mediated or tellurium mediated ligation. In another aspect, the invention provides for the use of fluorescence resonance energy transfer (FRET) to detect the nonenzymatic ligation....

20060160124 - Detection of negatively charged polymers using water-soluble, cationic, polythiophene derivatives: Novel methods allowing for the simple optical and electrochemical detection of double-stranded oligonucleotides are disclosed. The methods are rapid, selective and versatile. Advantageously, they do not require any chemical reaction on the probes or on the analytes since they are based on different electrostatic interactions between cationic poly(3-alkoxy-4-methylthiophene) derivatives and...

20060160083 - Diagnositics and therapeutics for diseases associated with human g-protein couple receptor 14 (gpr14): The invention provides a human GPR14 which is associated with the hematological diseases, cardiovascular diseases, disorders of the peripheral and central nervous system, urological diseases, endocrinological diseases and cancer. The invention also provides assays for the identification of compounds useful in the treatment or prevention of hematological diseases, cardiovascular diseases,...

20060160088 - Diagnostics and therapeutics for diseases associated with g protein-coupled receptor 40 (gpr40): The invention provides a human GPR40 which is associated with the hematological diseases, disorders of the peripheral and central nervous system, gastrointestinal diseases, respiratory diseases, metabolic disease, cancer, cardiovascular diseases and urological diseases. The invention also provides assays for the identification of compounds useful in the treatment or prevention of...

20060160100 - Enzymatic electrochemical detection assay using protective monolayer and device therefor: There is provided an electrochemical assay method for detecting a target molecule, for example a protein, in a sample, which involves the use of a protective monolayer and a redox polymer to form a bilayer immobilized on an electrode. The monolayer protects the electrode from non-specific adherence of reagents, particular...

20060160127 - Extraction of molecules using frame: Disclosed is the extraction of molecules from at least one separation medium wherein a frame with different compartments is brought in contact with the separation medium, thereby dividing the separation medium into different compartments. At least one solvent is applied into the different compartments, extracting the molecules....

20060160089 - Fixing agents, method of fixing substance with the same, and substrate having sustance fixed thereto with the same: An agent for fixing (a) substance(s), which can fix various substances to be fixed on a substrate via covalent bonds and which has an excellent effect for the prevention of non-specific adsorption is disclosed. A polymer containing a plurality of phosphorylcholine groups and photoreactive groups in one molecule is used....

20060160119 - Genetic screening for improving treatment of patients diagnosed with depression: Disclosed is a method for screening patients to determine whether or not SSRI therapy is likely to alleviate symptoms of depression in those patients. The method provides a polymorphism at position −1019 of the 5-HT1A gene that is predictive of likelihood of improvement of symptoms and a polymorphism at position...

20060160109 - Harnessing network biology to improve drug discovery: This invention provides principles, methods and compositions for ascertaining the mechanism of action of pharmacologically important compounds in the context of network biology, across the entire scope of the complex pathways of living cells. Importantly, the principles, methods and compositions provided allow a rapid assessment of the on-pathway and off-pathway...

20060160102 - Identification of rare alleles by enzymatic enrichment of mismatched heteroduplexes: In one aspect, the invention provides a method of enriching a pool of nucleic acid duplexes for heteroduplexes containing sequences that vary from those of a reference DNA population. A mixture of test sequences, that is, nucleic acids suspected of containing one or more mutations or rare alleles, and corresponding...

20060160101 - Inactivated fcv vaccines: The present invention relates to improved inactivated feline calicivirus (FCV) vaccines. The invention also provides a process for producing stabilized inactivated FCV, and the use of such stabilized inactivated FCV, in the production of FCV immunogenic compositions. The invention further provides methods of inducing an immune response in an animal...

20060160093 - Introduction of structural affinity handles as a tool in selective nucleic acid separations: The method is used for separating nucleic acids and other similar constructs. It involves selective introduction, enhancement, or stabilization of affinity handles such as single-strandedness in the undesired (or desired) nucleic acids as compared to the usual structure (e.g., double-strandedness) of the desired (or undesired) nucleic acids. The undesired (or...

20060160075 - Labelled nucleotides: Nucleosides and nucleotides are disclosed that are linked to detectable labels via a cleavable linker group....

20060160081 - Labelled nucleotides: The invention provides a nucleotide or nucleoside having a base attached to a detectable label via a cleavable linker, characterised in that the cleavable linker contains a moiety selected from the group comprising: Formula (I) wherein X is selected from the group comprising O, S, NH and NQ wherein Q...

20060160078 - Lateral flow assay device and method: Disclosed is a lateral flow assay device to test for the presence and/or amount of a nucleic acid sequence of interest in a sample, the lateral flow device comprising: (a) a sample receiving zone for contacting the device with a sample to be tested; (b) an extraction zone (8) for...

20060160103 - Mammalian neuralized family transcriptional regulators and uses: The disclosure relates to isolated polynucleotides and purified polypeptides of the Neu family of proteins, which have been shown to demonstrate transcriptional regulatory activity. For example, the purified polynucleotide can encode a Neu polypeptide, wherein the Neu polypeptide comprises at least one neuralized homology repeat domain and a C3HC4 RING-zinc...

20060160111 - Measuring forster resonance energy transfer with polarized and depolarized light: The present invention provides improved methods for assessing Förster resonance energy transfer using polarized light. Specifically, the methods rely on measuring depolarized light emitted by fluorescent acceptor molecules....

20060160106 - Method and probes for the detection of chromosome aberrations: A novel method for detecting chromosome aberrations is disclosed. More specifically, chromosome aberrations are detected by in situ hybridisation using at least two sets of hybridisation probes, at least one set comprising one or more peptide nucleic acid probes capable of hybridising to specific nucleic acid sequences related to a...

20060160077 - Method for analyzing the spatial distribution of a chemical substance retained by a biological matter: A method of analyzing the spatial distribution of at least one chemical substance retained by a biological matter comprises the steps of (a) supplying a sample of said biological matter as a specimen surface; (b) producing at least one imprint of said specimen surface on at least one corresponding separate...

20060160086 - Method for continuous detection of an analyte, trifunctional detecting reagent used and detecting device: The invention concerns a method for heterogeneous phase detection of an analyte, the trifunctional reagent used for implementing said method, its use for detecting an analyte, as well as the device for detecting a corresponding analyte...

20060160123 - Method of minimizing off-target effects of sirna molecules: The invention relates to a method of minimizing off-target effects of siRNA, comprising preparing double-stranded RNA (dsRNA) having a sense strand that is homologous to a sequence of a target gene and an anti-sense strand that is complementary to said sense strand, and having at least one pyrimidine replaced by...

20060160105 - Methods for detection of genetic disorders: The invention provides a method useful for detection of genetic disorders. The method comprises determining the sequence of alleles of a locus of interest, and quantitating a ratio for the alleles at the locus of interest, wherein the ratio indicates the presence or absence of a chromosomal abnormality. The present...

20060160104 - Methods for diagnosing, preventing, and treating developmental disorders due to a combination of genetic and environmental factors: The present invention discloses a novel method for identifying an individual who may be susceptible to develop a developmental disorder. In one particular example, an individual is identified who is genetically susceptible to becoming schizophrenic. In addition, the present invention discloses a novel method for identifying individuals who are genetically...

20060160094 - Methods for restoring wild-type p53 gene function: Methods are provided for restoring wild-type p53 gene function to a cell. Such methods include gene therapy. Typically, this will stop tumor cells from proliferating....

20060160079 - Methods for the treatment of dementia based on apo e genotype: This invention relates to methods to prevent worsening of and/or to improve cognitive functioning and behaviour problems in patients with dementia by means of ApoE genotyping to guide the use of AChEI drugs, including rivastigmine. Also included are kits for determining ApoE4 status and recommended treatment strategy....

20060160110 - Methods of designing small interfering rnas, antisense polynucleotides, and other hybridizing polynucleotides: The present invention relates to methods, apparatus and computer program products for selecting siRNAs, antisense polynucleotides, and other hybridizing polynucleotides. In particular, the invention relates to methods for selecting siRNAs, antisense polynucleotides, and other hybridizing polynucleotides that have moderate or low off-target activity....

20060160129 - Methods of producing a library and methods of selecting polynucleotides of interest: The present invention relates to a high efficiency method of introducing DNA into linear DNA viruses such as poxvirus, a method of producing libraries in linear DNA viruses such as poxvirus, and methods of selecting polynucleotides of interest based on cell nonviability or other phenotypes....

20060160107 - Methods of screening for compounds that improve engraftment: The invention provides for methods of screening for compounds that increase the expression of P-selectin, SDF-1, and/or CXCR4 on facilitatory cells (FCs). The invention also provides for methods of screening for compounds that increase the level of p-predendritic cells (p-pre DC) without substantially decreasing the level of natural killer (NK)...

20060160076 - Methods of treating and diagnosing diabetes with cx3cr1 modulators: The present invention provides compositions and methods for diagnosing and treating diabetes and insulin resistance. In particular, the invention provides methods of identifying modulators of CX3CR1 and using those modulators to treat diabetes, as well as methods of diagnosing diabetes by measuring the levels of CX3CR1 or fractalkine in a...

20060160118 - Modulators of nod2 signaling: The present invention relates to intracellular signaling molecules, in particular the Nod2 protein and nucleic acids encoding the Nod2 protein. The present invention provides methods of identifying modulators of Nod2 signaling. In particular, the present invention additionally provides methods of screening immune modulators such as adjuvants using Nod2. The present...

20060160087 - Monitoring and treatment of amyotrophic lateral sclerosis: The invention provides methods of monitoring amyotrophic lateral sclerosis (ALS) disease development or progression and monitoring an ALS therapy in an individual by determining the presence or absence of Herv-K/HML-2 expression in a biological sample from the individual. The invention is also directed to methods for aiding diagnosis of ALS...

20060160128 - Novel adenosine deaminase homolog: This invention provides a novel isolated polynucleotide sequence, called ADARP1 (adenosine deaminase related protein 1), that displays significant homology to the adenosine deaminase (ADA) gene. Also provided is the amino acid sequence of the ADARP1 polypeptide encoded by the polynucleotide of the invention. The RNA for this novel gene is...

20060160085 - Novel buffer formulations for isolating purifying and recovering long-chain and short-chain nucleic acids: The invention relates to novel formulations of buffers used for isolating, purifying and recovering long-chain and short-chain nucleic acids. The areas of application of the inventive method include all laboratories engaged in isolating nucleic acids, such as laboratories used in forensic medicine, food diagnosis, medical diagnosis, molecular biology, biochemistry, genetic...

20060160082 - Novel genes and proteins encoded thereby: A novel DNA comprising a protein-encoding region is directly cloned from cDNA library originating from human adult whole brain and human fetal whole brain, the base sequence thereof is determined, and the functions thereof are identified. A DNA comprising a base sequence encoding the following polypeptide (a) or (b): (a)...

20060160120 - Novel hydrogel copolymer, substrate coated with the copolymer, method of producing microarray using the copolymer, and microarray produced by the method: A novel hydrogel copolymer, a substrate coated with the copolymer, a method of producing a microarray using the copolymer, and a microarray produced by the method are provided. The use of the hydrogel copolymer makes efficient removal of protein and high integration of nucleic acid and protein on a substrate...

20060160096 - Nucleic acid labeling methods: In one aspect of the invention, a method is provided for end-labeling RNA (total RNA, mRNA, cRNA or fragmented RNA). In one aspect of the present invention, T4 RNA ligase is used to attach a 3′-labeled AMP or CMP donor to an RNA acceptor molecule. In another embodiment, a pyrophosphate...

20060160074 - Pharmacogenetic dme detection assay methods and kits: The present invention relates to methods for detecting polymorphisms in enzymes related to drug metabolizm (Drug Metabolizing Enzymes or DMEs) such as uridine diphosphate glucuronosyl transferase (UGT) gene promoter, cytochrome p450, with a non-amplified oligonucleotide detection assays. The present invention also relates to pharmacogenetic DME detection assay kits....

20060160122 - Polyelectrolyte-coated size-exclusion ion-exchange particles: A polyelectrolyte-coated particle, devices for using the particle, methods for using the particle for separating PCR reaction products and/or DNA sequencing reaction products, and compositions for coating the particle are provided....

20060160098 - Polymeric label molecules: A method of constructing a highly labeled linear polymeric molecule for use in any desired application, and linear polymeric molecules of such structure. The polymeric molecule is a nucleic acid constructed from a large number of one or more types of monomeric oligonucleotide units that are attached together to form...

20060160117 - Polynucleotide motor, a motor system, their preparation and uses: A polynucleotide motor is disclosed, comprising an enzyme capable of binding to a duplex nucleic acid sequence, which enzyme is also capable of translocating the nucleic acid sequence without causing cleavage thereof. The motor may be associated with a substance bound to the nucleic acid sequence so that the bound...

20060160091 - Polynucleotides encoding a class ii cytokine receptor: Novel receptor polypeptides, polynucleotides encoding the polypeptides, and related compositions and methods are disclosed. The polypeptides comprise an extracellular domain of a cell-surface receptor that is expressed in kidneys, pancreas, prostate, adrenal cortex and nervous tissue. The polypeptides may be used within methods for detecting ligands that promote the proliferation...

20060160108 - Populations of reporter sequences and methods of their use: Compositions, methods and kits are provided that are useful, for example, for determining activities of multiple cis-regulatory sequences, such as promoters and enhancers, and/or multiple trans-acting factors, such as transcription factors, in a cell. In particular, in certain embodiments, compositions are provided comprising a population of polynucleotide reporter transcription units...

20060160121 - Probe arrays for detecting multiple strains of different species: The present invention provides probe arrays and methods of using the same for concurrent and discriminable detection of multiple strains of different species. In one aspect, the probe arrays of the present invention are nucleic acid arrays comprising (1) a first group of probes, each of which is specific to...

20060160084 - Process for amplifying nucleic acid: The present invention relates to a process for synthesizing or amplifying efficiently a nucleic acid comprising a target nucleic acid sequence. In the process according to the present invention, a primer comprising in its 3′-end portion a sequence (Ac′) which hybridizes a sequence (A) in the 3′-end portion of the...

20060160114 - Reagents and methods for predicting drug resistance: The invention provides methods for prognosis, diagnosis, staging and determining disease progression in human cancer patients related to expression levels of one or a plurality of genes or genetic loci that are differentially deleted, amplified, expressed or amplified and over-expressed in chemotherapeutic drug resistant tumor cells....

20060160130 - Recombinant rsv virus expression systems and vaccines: The present invention relates to genetically engineered recombinant RS viruses and viral vectors which contain heterologous genes which for the use as vaccines. In accordance with the present invention, the recombinant RS viral vectors and viruses are engineered to contain heterologous genes, including genes of other viruses, pathogens, cellular genes,...

20060160116 - Repetitive sequence-free dna libraries: A method of creating a repetitive sequence-free DNA library comprising the steps of providing a DNA library, providing an amplification mixture from the DNA library, and adding a repetitive sequence fraction DNA to the amplification mixture to produce the repetitive sequence-free DNA library. The invention also provides a method of...

20060160099 - Substrate preparation process: The present invention provides novel processes for the large scale preparation of arrays of polymer sequences wherein each array includes a plurality of different, positionally distinct polymer sequences having known monomer sequences. The methods of the invention combine high throughput process steps with high resolution photolithographic techniques in the manufacture...

20060160095 - Survivin: The invention provides a method for detecting apoptosis in a myeloid cell comprising detecting an alteration in any one of: i) a Survivin polypeptide having an amino acid sequence as set out in SEQ ID NO: 1; ii) a polypeptide having at least 80% homology with i); iii) a nucleic...

20060160113 - Terminal-phosphate-labeled nucleotides: The present invention is directed to a method of sequencing a target nucleic acid molecule having a plurality of bases. In its principle, the temporal order of base additions during the polymerization reaction is measured on a molecule of nucleic acid, i.e. the activity of a nucleic acid polymerizing enzyme...

20060160112 - Tissue inhibitor of metalloproteinase (timp) as a marker and predictor of cardiovascular disease and both cardiac and non-cardiac mortality: The present invention is directed to a method for characterizing an individual's risk profile of developing vascular disease, for an adverse outcome in a patient, for mortality in an individual diagnosed with vascular disease by evaluating the level of tissue inhibitor of metalloproteinase-1 (TIMP-1) in a sample taken from the...

20060160138 - Compositions and methods for protein design: In certain aspects the present invention provides methods and compositions related to rational protein design....

20060160132 - Diagnositics and therapeutics for diseases associated with g-protein coupled receptor hm74a (hm74a): The invention provides a human HM74a which is associated with the disorders of the peripheral and central nervous system, cardiovascular diseases, hematological diseases, cancer, inflammation, urological diseases, respiratory diseases and gastroen-terological diseases. The invention also provides assays for the identification of compounds useful in the treatment or prevention of disorders...

20060160145 - Epf receptor assays, compounds and therapeutic compositions: The present invention provides assays for the study of the interaction of EPF and its EPF-related peptides with a hDRR receptor. The assays are useful to identify whether a test compound can bind to the hDRR receptor under conditions in which EPF or related peptide can bind to the receptor....

20060160133 - Glucose-transport related genes, polypeptides, and methods of use thereof: Methods and compositions for modulating glucose transport are provided herein....

20060160136 - High throughput screening of catalysts using spin resonance: High throughput screening of catalyst libraries may be performed using spin resonance techniques, and an evanescent wave probe developed by the present inventors. The probe may operate using either nuclear magnetic resonance or electron spin resonance techniques. In one configuration, a scanning evanescent wave spin resonance probe is used in...

20060160140 - Human nuclear receptor protein nnr7-1: The present invention discloses the isolation and characterization of cDNA molecules encoding a novel member to the human nuclear receptor superfamiliy, designated nNR7 and/or nNR7-1. Also within the scope of the disclosure are recombinant vectors, recombinant host cells, methods of screening for modulators of nNR7 and/or nNR7-1 activity, and production...

20060160137 - Method for modification of a synthetically generated assay using measured whole crude properties: The present invention is a method for modifying any synthetically generated assay of a whole crude oil or a portion of a whole crude, such as a condensate or resid material, by using measured crude properties. These measured properties are used to adjust the synthesized assay values to obtain a...

20060160131 - Method for the identification of drug targets: The present invention relates to the field of drug development. More specifically the invention provides a method for the identification of drug targets. The method can also be used for analysis of proteomes. The method utilizes in essence a combination of two chromatographic separations of the same type, separated by...

20060160143 - Mitogenic oxygenases: The present invention relates to new genes encoding for the production of novel proteins involved in generation of reactive oxygen intermediates that affect cell division. The present invention also provides vectors containing these genes, cells transfected with these vectors, antibodies raised against these novel proteins, kits for detection, localization and...

20060160134 - Novel application of biosensors for diagnosis and treatment of disease: A method for detecting compounds of interest in bodily fluids, including exhaled breath and blood. The present invention uses biosensors that mimic naturally occurring cellular mechanisms, including RNA oligonucleotide chains or “aptamers,” in combination with signaling agents or nanotechnology to provide an effective and efficient method for diagnosing a condition...

20060160141 - Peptide sequence tags and method of using same: Peptide sequence tags are identified and used to produce a class of global antibodies, which recognize all members of PsbB protein family with uniform specificity, regardless of the species of origin. The tags are used to create antibodies to the stable PsbB protein of the Photosystem II complex catalyzing photochemical...

20060160142 - Phosophoinositoglycan binding proteins: The invention refers to a protein from plasma membrane of adipocytes. The protein has specific binding affinity to phosphoinositoylglycans. It regulates glucose uptake by circumventing the insulin signaling cascade....

20060160139 - Rhesus bcrp and antibodies thereto: The present invention provides a breast cancer resistance polynucleotide and protein of Macaca mulata (rhesus monkey) and uses thereof. The present invention also provides anti-BCRP antibodies and uses thereof, particularly as a hematopoietic stem cell marker....

20060160144 - Sensor arrangement, device and method for testing active substances and/or active sites from a pharmacological point of view using an amperometer and/or potentiometer: The invention relates to a sensor electrode device (20) for carrying out amperometric and/or potentiometric testing of active sites or substances from a pharmacological point of view. In order to carry out testing in a reliable, quick and inexpensive manner, the device is provided with a solid-supported electrode area (21)...

20060160135 - Sf-1 and lrh-1 modulator development: Structures of SF1 and LRH are described, along with methods for identifying or developing modulators of those receptors and uses for such modulators....

20060160149 - G-coupled receptor showing selective affinity for atp: The present invention relates to an isolated G-protein coupled receptor, nucleic acid sequence encoding the receptor, and host cells capable of expressing the receptor. The invention further comprises methods for detecting the expression of a G-protein coupled receptor, and methods for identifying agonists or antagonists of the receptor. The invention...

20060160150 - Immunoassays for brain natriuretic propeptide: Immunoassays for the biological precursor of human brain natriuretic peptide are described....

20060160146 - Method of screening compound affecting amyloid beta production: It is intended to provide a material for efficiently measuring the production of β-amyloid which relates to the onset of Alzheimer's type dementia, and a method of efficiently screening a substance affecting the production of β-amyloid by using the above material....

20060160148 - Optoelectronic sensor: A device for detecting the presence of an antigen including (1) a cell having antibodies which are expressed on the surface of the cell and are specific for the antigen to be detected, where binding of the antigen to the antibodies results in an increase in calcium concentration in the...

20060160147 - Recombinant cell line: The invention relates to a recombinant cell line capable of inducible expression of an α and/or β subunit of interleukin 12 (IL-12), and an ecdosymeinducible expression vector capable of transfecting a host cell to produce the recombinant cell line of the invention. The invention also relates to a method of...

20060160153 - Method to measure a t cell response and its uses to qualify antigen-presenting cells: A method to characterize a T-cell response of a final population of T-lymphocytes resulting from the co-incubation of an initial population of T lymphocytes with a composition of antigen-presenting cells (APCs), said method comprising the steps of a) simultaneous measuring on a single cell basis at least two parameters: (i)...

20060160154 - Materials and methods for detection and treatment of breast cancer: The invention provides a wide range of methods and compositions for detecting and treating breast cancer in an individual. Specifically, the invention provides target breast cancer-associated proteins, which permit a rapid detection, preferably before metastases occur, of breast cancer. The target breast cancer-associated protein may be detected, for example, by...

20060160157 - Method, compositions and classification for tumor diagnostics and treatment: The present invention is directed towards classifying tumor biomarkers, particularly membrane receptors, and more particularly the gastrin-releasing peptide (GPR) receptors, identified in patient samples, then linking therapeutic agents (chemical, radiological, or biological) to patient-specific ligands that bind to such receptors, clinicians can produce diagnostic and treatment compositions and implement treatment...

20060160155 - Serum levels of her2/neu as an indicator of clinical state and prognosis of prostate cancer: The present invention relates to methods of diagnosing prostate cancer, predicting disease progression of prostate cancer, prognosing the stage and state of prostate cancer, and identifying appropriate treatment indication for prostate cancer patients. Methods encompass determining the levels of HER-2/neu in fluid biological samples (e.g., blood and serum) from patients....

20060160156 - Tears as a screening medium: The present invention is directed to the use of surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF-MS) on tears of breast cancer patients. The present invention demonstrates the use of this highly sensitive and specific method to identify proteins and protein patterns in tears that show promise as a diagnostic indicator....

20060160159 - Identification of actinobacillus actinomycetemcomitans antigens for use in the diagnosis, treatment, and monitoring of periodontal diseases: Antibodies, polypeptides, and polynucleotides are provided for the detection, prevention, amelioration and treatment of diseases caused by Actinobacillus actinomycetemcomitans....

20060160158 - Target and method for inhibition of bacterial rna polymerase: t

20060160151 - Method of quantitative immunohistochemistry and in situ hybridization: The present invention involves a simple and rapid method for quantitative detection of a ligand in a sample using a combination of an ELISA-like assay and immunohistochemical staining....

20060160152 - Purified stat proteins and methods of purifying thereof: The present invention describes methods of producing milligram quantities of three forms of purified Stat1 protein from recombinant DNA constructs. In addition, the Stat proteins may be isolated in their phosphorylated or nonphosphorylated forms (Tyr 701). The proteins can be produced in baculovirus infected insect cells, or E. coli. A...

20060160162 - Capillary array and related methods: The invention provides methods and devices for detecting the presence of one or more target analytes in a sample employing a channel having affixed therein one or more binding partners for each target analyte. Assays are carried out by transporting the sample through the channel to each successive binding partner...

20060160160 - Detection of human antibodies to squalene in serum: The invention is a method and assay for detecting human squalene antibodies in sera or plasma using a blocking agent of −0.5% casein in a phosphate buffered saline (PBS) solution....

20060160161 - Methods for assessing antibodies to neurodegenerative disease-associated antigens: The invention provides methods for assessing immunoglobulins directed to neurodegenerative disease-associated antigens. Methods of the instant invention involve specific, sensitive and precise identification of Ig classes of antibodies present in a test sample that are capable of binding an immobilized neurodegenerative disease-associated antigen. The enhanced assessment of antibodies directed to...

20060160164 - Immunoassay device with immuno-reference electrode: An electrochemical immunosensor system with reduced interference, comprising: a first immunosensor that generates an electrochemical signal based on the formation of a sandwich between an immobilized antibody, a target analyte and a labeled antibody, wherein a portion of the signal arises from non-specific binding of the labeled antibody in the...

20060160163 - Method of saying alastin digestion product and assay kit, method of detecting aortic dissection and detection kit: An immunoassay method for conveniently and accurately measuring amount of the elastin degradation product in a circulating fluid of a subject, which is useful for detection of aortic dissection, the method comprising immunologically binding a first antibody and a second antibody to the elastin degradation product, wherein the first antibody...

20060160165 - Device and methods for identifying and treating aspirin non-responsive patients: The present invention relates to methods and compositions for identifying and treating subjects in need of antithrombotic therapies but who are not responsive to aspirin....

20060160166 - Rapid triglyceride assay for use in pulp pitch control: Enzymatic methods are provided for determining the surface triglycerides content in a sample of wood pulp. The methods preferably comprise reacting triglycerides which are present on the surface of the wood pulp fibers in the sample in the presence of a lipase to form glycerol and fatty acids, and then...

20060160167 - Screening assay for enzymes involved in pathophysiology using seldi-tof mass spectrometry: The invention provides methods for quantifying enzymatic activity of an enzyme with a known substrate. The methods employ SELDI-TOF mass spectrometry, and are suitable, in particular, for assaying aspects of the renin-angiotensin system. The methods may be utilized to assess and/or monitor biological conditions associated with the renin-angiotensin system prior...

20060160168 - Methods and products for identifying compounds that modulate cell plasma membrane repair: The invention relates in some aspects to methods and kits for determining plasma membrane repair status in cells, tissues, and subjects. The methods include, in part, the use of high-throughput assays for assessing cell plasma membrane repair status. The methods and kits of the invention are useful to identify compounds...

20060160169 - Cell microarray for profiling of cellular phenotypes and gene function: The present invention includes compositions, methods and systems for analyzing one or more cell characteristics including the steps of depositing one or more spots on a substrate that include one or more cells from a strain collection, contacting the one or more cells with one or more agents and evaluating...

20060160171 - Detection method and apparatus for detecting microbial growth: A system and method is provided for non-invasively measuring changes in a specimen suspected of containing one or more microbes, by monitoring changes in the dielectric constant of the specimen caused by metabolic processes of such microbes....

20060160170 - Method and device of rapid antigen extraction: A system and a method of the interaction and preservation of two or more reagents used in a chemical reaction is described, in which the reagents are put in contact one with another only when a wad (A) carrying a sample to be treated with said reagents breaks a partition...

20060160172 - Sqs gene: The present invention relates to a gene useful in a process to increase the microbial production of carotenoids. The carotenoids astaxanthin is distributed in a wide variety of organisms such as animals, algae and microorganisms. It has a strong antioxidation property against reactive oxygen species. Astaxanthin is used as a...

20060160175 - Compositions of orthogonal leucyl-trna and aminoacyl-trna synthetase pairs and uses thereof: Compositions and methods of producing components of protein biosynthetic machinery that include leucyl orthogonal tRNAs, leucyl orthogonal aminoacyl-tRNA synthetases, and orthogonal pairs of leucyl tRNAs/synthetases are provided. Methods for identifying these orthogonal pairs are also provided along with methods of producing proteins using these orthogonal pairs....

20060160184 - Fab library for the preparation of anti vegf and anti rabies virus fabs: The present invention provides combinations of specific binding proteins, such as immunoglobulins, that are designed to be true combinations, essentially all components of the combination being functional and compatible with each other. The invention further provides a method for producing a composition comprising at least two different proteinaceous molecules comprising...

20060160173 - Feedback-resistant homoserine transsuccinylases having a modified c terminus: The invention relates to a homoserine transsuccinylase, which exhibits reduced sensitivity towards L-methionine or SAM in comparison with a homoserine transsuccinylase wild-type enzyme, whereby the latter comprises an amino acid sequence containing a TyrGlnXaaThrPro sub-sequence, the Thr of said sub-sequence lying between positions 285 and 310 of the amino acid...

20060160181 - Fibroblast growth factor-23 molecules and uses thereof: The present invention provides Fibroblast Growth Factor-23 (FGF-23) polypeptides and nucleic acid molecules encoding the same. The invention also provides selective binding agents, vectors, host cells, and methods for producing FGF-23 polypeptides. The invention further provides pharmaceutical compositions and methods for the diagnosis, treatment, amelioration, and/or prevention of diseases, disorders,...

20060160176 - Functional assays for identifying compounds that modulate t1r1/t1r3 (umami) taste: Functional assays for identifying compounds that activate or modulate the activation of the T1R1/T1R3 (umami) taste receptor are provided. These assays detect the effect of one or more compounds on the activation of T1R1/T1R3 (umami) taste receptor or on the activation of T1R1/T1R3 umami taste receptor by another compound e.g.,...

20060160179 - Methods for eliminating mannosylphosphorylation of glycans in the production of glycoproteins: The present invention relates to the elimination of mannosylphosphorylation on the glycans of glycoproteins in the yeast genus Pichia. The elimination of mannosylphosphorylated glycoproteins results from the disruption of the PNO1 gene and the newly isolated P. pastoris MNN4B gene. The present invention further relates to methods for producing modified...

20060160185 - Methods of evaluating viral entry inhibitors using patient derived envelope protein constructs: The invention provides a method for identifying whether a compound inhibits entry of a virus into a cell which comprises: (a) obtaining nucleic acid encoding a viral envelope protein from a patient infected by the virus; (b) co-transfecting into a first cell (i) the nucleic acid of step (a), and...

20060160178 - Methods of generating antibody diversity in vitro: The present invention provides a high throughput method for generating fully human monoclonal antibodies....

20060160174 - Modified l49-sfv exhibiting increased stability and methods of use thereof: The present invention relates to a modified L49 single chain antibody (L49-sFv) that exhibits increased refolding efficiency and/or greater stability in mouse serum, and surprisingly substantially maintains binding affinity for its binding ligand, p97 melanotransferrin. p97 melanotransferrin is expressed on the surface of a number of types of cancer (carcinoma)...

20060160186 - Nucleic acid underexpressed in stomach tumor and lung tumor: The present invention is directed to novel polypeptides and to nucleic acid molecules encoding those polypeptides. Also provided herein are vectors and host cells comprising those nucleic acid sequences, chimeric polypeptide molecules comprising the polypeptides of the present invention fused to heterologous polypeptide sequences, antibodies which bind to the polypeptides...

20060160177 - Peptide extended glycosylated polypeptides: Glycosylated polypeptides comprising the primary structure NH2-X-Pp-COOH, wherein X is a peptide addition comprising or contributing to a glycosylation site, and Pp is a polypeptide of interest or comprising the primary structure NH2-Px-X-Py-COOH, wherein Px is an N-terminal part of a polypeptide Pp of interest, Py is a C-terminal part...

20060160180 - Production of alpha-abeta: An improved system for large scale production of proteins and/or polypeptides in cell culture, particularly in media characterized by one or more of: i) a cumulative amino acid concentration greater than about 70 mM; ii) a molar cumulative glutamine to cumulative asparagine ratio of less than about 2; iii) a...

20060160182 - Use of rna trans-splicing for antibody gene transfer and antibody polypeptide production: The present invention provides methods and compositions for generating novel nucleic acid molecules through RNA trans-splicing that target a highly expressed pre-mRNA and contain the coding sequence for antibody polypeptide(s). The compositions of the invention include pre-trans-splicing molecules (PTMs) designed to interact with the target precursor messenger RNA molecule (target...

20060160183 - Vaccines and immunotherapeutics derived from the human immunodeficiency virus (hiv) transactivator of transcription protein for the treatment and prevention of hiv disease: Anti-lentivirus vaccines and immunotherapeutics and methods for preparing and using same are disclosed. The vaccines and immunotherapeutics are produced using non-immunosuppressive lentivirus trans-activator of transcription (Tat) proteins. An associated in vitro ultra-sensitive macrophage Tat bioassay is disclosed for assessing the immunosuppressive qualities of the lentivirus Tat preparations of the present...

20060160187 - Combinatorial interleukin-2 muteins: Novel human interleukin-2 (IL-2) muteins or variants thereof, and nucleic acid molecules and variants thereof are provided. Methods for producing these muteins as well as methods for stimulating the immune system of an animal are also disclosed. In addition, the invention provides recombinant expression vectors comprising the nucleic acid molecules...

20060160188 - Systems, methods , and compositions for detection of human papilloma virus in biological samples: The present invention comprises, without limitation, systems, methods, and compositions for the detection, identification, and quantification, down to the single copy level, of human papillomavirus (HPV) in biological samples, including but not limited to, mammalian body fluids and cervix scrapings, for purposes of detection, treatment and/or management of cancer and...

20060160189 - High-temperature enzyme starch-to-sugar conversion: A starch-to-sugars conversion method comprises mixing rice flour with water, alpha-amylase enzyme, and calcium chloride into a slurry with 27% solids. The slurry is liquefied at about 223° F. Glucoamylase and beta-amylase enzymes are added and the slurry is incubated. The action of the three enzymes is terminated. The slurry...

20060160190 - Method and equipment for producing glucose from a starch solution: A method for producing glucose from a starch solution includes a step for saccharification of the starch solution (30) by adding enzymes and/or acid to crude syrup. The method then includes separation of a phase of proteins and/or fats from the crude syrup in a centrifugal separating device (110) and...

20060160192 - A method for producing an l-amino acid using a bacterium of the enterobacteriaceae family having a pathway of glycogen biosynthesis disrupted: There is provided a method for producing an L-amino acid using a bacterium of the Enterobacteriaceae family, particularly a bacterium belonging to the genus Escherichia or Pantoea, having the glycogen biosynthesis pathway disrupted....

20060160191 - L-amino acid producing microorganism and a method for producing l-amino acid: An L-amino acid producing bacterium of the Enterobacteriaceae family is described, wherein the bacterium has been modified so as to not produce type I fimbrial adhesin protein is cultured in a medium to produce and excrete said L-amino acid in the medium, and collecting said L-amino acid from the medium....

20060160193 - Acyltransferases for alteration of polyunsaturated fatty acids and oil content in oleaginous yeasts: Two acyltransferases are provided, suitable for use in the manufacture of microbial oils enriched in omega fatty acids in oleaginous yeast (e.g., Yarrowia lipolytica). Specifically, the genes encoding phophatidylcholine-diacylglycerol acyltransferase (PDAT) and diacylglycerol acyltransferase (DGAT2) have been isolated from Y. lipolytica. These genes encode enzymes that participate in the terminal...

20060160195 - Novel vector: It is intended to provide a process for industrially safely and/or economically and efficiently producing a polyester by using gene manipulation techniques. A vector applicable to hosts over a wide range which is characterized by having improved industrial safety; a polyester synthase-expressing plasmid wherein at least a polyester synthase gene...

20060160194 - Process for the production of biodegradeable, functionalised polymer particles, and use thereof as pharmaceutical supports: The invention relates to a method for producing biodegradable, functionalised polymer particles, and to the use of the same as medicament carriers....

20060160198 - Method for the production of glycolic acid from ammonium glycolate by direct deammoniation: The present invention relates to a method for obtaining glycolic acid by direct deammoniation of ammonium glycolate. More specifically, water is removed from an aqueous solution of ammonium glycolate to produce the substantially anhydrous salt. Thermal salt cracking is then used to remove ammonia from the salt, producing a deammoniated...

20060160197 - Method for the production of glycolic acid from ammonium glycolate by solvent extraction: The present invention relates to a method for producing glycolic acid from an aqueous solution of ammonium glycolate using reactive solvent extraction. More specifically, an aqueous solution of glycolic acid is created by acidifying an aqueous ammonium glycolate solution. An organic extraction solution comprising a tertiary trialkylamine is contacted with...

20060160196 - Process for the synthesis of glycolonitrile: A process to prepare substantially pure glycolonitrile in an aqueous medium is provided by reacting hydrogen cyanide and formaldehyde. The formaldehyde feed stream is heated prior to reacting with hydrogen cyanide, resulting in an aqueous glycolonitrile solution with fewer impurities, especially less unreacted formaldehyde, than is obtained by other methods....

20060160199 - Enzymatic production of glycolic acid: Various methods are provided for the enzymatic production of glycolic acid from glycolonitrile. These methods include: 1) use of Acidovorax facilis 72W nitrilase mutants having improved nitrilase activity for converting glycolonitrile to glycolic acid, and 2) methods to improve catalyst stability and/or productivity. The methods to improve catalyst stability/productivity include...

20060160200 - Supporting body with immobilized catalytically active units: The present invention relates to the use of porous carbon-based bodies for the support and/or immobilization of catalytically active units. Catalytically active units for chemical and/or biological reactions may be essentially immobilized on such supporting bodies. The catalyst units can comprise catalytically active units and porous carbon-based supporting bodies. The...

20060160201 - Three-dimensional structures of hdac9 and cabin1 and compound structures and methods related thereto: Disclosed are the three-dimensional structures of two complexes: a Cabin1/MEF2B/DNA complex and a MITR/MEF2B/DNA complex. Also disclosed are methods of using such structures and models derived therefrom in structure-based design methods to identify regulators of the interaction of MEF2 with its cognate ligands/corepressors, to compounds that can be designed or...

20060160202 - Artificial chromosomes comprising ehv sequences: The invention belongs to the field of animal health, in particular equine diseases caused by equine herpesvirus (EHV). The invention relates to artificial chromosomes comprising the genome of equine herpesviruses, methods of producing attenuated or virulent EHV with or without the insertion of foreign genes, EHV obtainable with said methods...

20060160203 - Process for the heterotrophic production of microbial products with high concentrations of omega-3 highly unsaturated fatty acids: Disclosed is a process for growing the microflora Thraustochytrium, Schizochytrium, and mixtures thereof, which includes the growing of the microflora in fermentation medium containing non-chloride containing sodium salts, in particular sodium sulfate. In a preferred embodiment of the present invention, the process produces microflora having a cell aggregate size useful...

20060160204 - Systems and methods for ex-vivo organ care: The invention provides, in various embodiments, systems, devices and methods relating to ex-vivo organ care. In certain embodiments, the invention relates to maintaining an organ ex-vivo at near-physiologic conditions....

20060160210 - Biological sample analysis plate: In a biological sample analysis plate, one ends of channels 11 and 12 are connected to a quantitative sorting part 10 having a predetermined volume for taking a predetermined amount of a biological sample. On the other end of the channel 11, a buffer agent storage part 7 for holding...

20060160207 - Cucurbituril derivative-bonded solid substrate and biochip using the same: The present disclosure provides a cucurbituril derivative-bonded solid substrate in which cucurbituril derivatives of Formula 1 are covalently bonded to a modified solid substrate of Formula 2. The cucurbituril derivative provides functional groups which can be used to link biomaterials to the substrate. Protein chips, gene chips, and sensors for...

20060160205 - Devices and methods for biochip multiplexing: The invention is directed to devices that allow for simultaneous multiple biochip analysis. In particular, the devices are configured to hold multiple cartridges comprising biochips comprising arrays such as nucleic acid arrays, and allow for high throughput analysis of samples....

20060160208 - Multi-well plate with alignment grooves for encoded microparticles: A method and apparatus are provided for aligning optical elements or microbeads, wherein each microbead has an elongated body with a code embedded therein along a longitudinal axis thereof to be read by a code reading device. The microbeads are aligned with a positioning device so the longitudinal axis of...

20060160206 - Parallel prosessing of microfluidic devices: Microfluidic arrangement which comprises A) a number of microfluidic devices, and B) an instrument which comprises a spinner motor and a rotary member arranged such that liquid flow can be driven centrifugal force in each of the devices by spinning the. Each of the microfluidic devices comprises microchannel structures in...

20060160209 - Systems and methods for measurement optimization: The invention relates to methods and systems for detecting and or analyzing an agent in a sample with a chip having optical components incorporated therein....

20060160211 - Transgenic mice containing, cgmp phosphodiesterase gene disruptions: The present invention relates to transgenic animals, as well as compositions and methods relating to the characterization of gene function. Specifically, the present invention provides transgenic mice comprising disruptions in cGMP phosphodiesterase genes. Such transgenic mice are useful as models for disease and for identifying agents that modulate gene expression...

20060160213 - Enhancement of mammalian embryo development: A method of enhancing in vitro development of a mammalian embryo is disclosed which comprises supplementing the culture medium with a prostaglandin, or a prostaglandin analog, in an amount effective to promote complete hatching of the embryo (i.e., freeing of the embryo from the zona pellucida). The quality of human...

20060160212 - Process for isolating haemocyanin: A process for obtaining a substantially pure, stable solution of a molluscan haemocyanin, comprising the steps of: (1) collecting blood from a mollusc; (2) centrifuging the collected blood to remove cellular material and other particulates to obtain a serum fraction; (3) diafiltering the serum fraction to replace serum liquid with...

20060160214 - Engineered intervertebral disc tissue: Surgically implantable tissue engineered intervertebral disc tissues that effectively replicate the physicochemical properties of the corresponding in vivo tissues are provided. Methods for producing such tissues can involve culturing the intervertebral disc cells to produce cells surrounded by an extracellular matrix and culturing the cells and matrix on a semipermeable...

20060160215 - Methods of developing a stem cell line: The invention relates to a methodology for creating an embryonic stem cell line. The stem cells derived from the invention are of substantially better quality of human stem cells currently known to exist. The inventors have discovered inter alia, that when the chromosome complement of a first polar body (PB-I)...

20060160216 - Human liver cell line: In this application is described the establishment and maintanence of a normal human hepatocyte cell line able to support complete development of malaria parasite development in vitro. Advantages and uses of the cell line are also described....

20060160217 - Novel nucleic acids from sesame plants: Novel nucleic acids derived from the 5′ untranslated regions of the 2S albumin and 11S globulin genes from sesame are disclosed. These novel nucleic acids can be used to direct expression of coding nucleic acids. Expression constructs can be introduced into a plant cell to generate a transgenic plant cell...

20060160219 - Method of transferring mutation into target nucleic acid: A method of transferring a mutation into the base sequence of a target nucleic acid characterized by comprising the step of preparing a DNA having a reversed repetitive sequence wherein the base sequence of the DNA having the reversed repetitive sequence is homologous with the target nucleic acid and has...

20060160218 - Transdifferentiation of cells and tissues: Method for carrying out transdifferentiation of non-pancreatic cells to pancreatic cells involving the provision of a pancreas specific transcription factor and an activating means such as VP16 of Herpes simplex virus to non-pancreatic cells. The methods and materials provided by the invention may be used to treat pancreatic disorders, in...

20060160220 - Retroviral vectors with introns: The present invention relates to improved retroviral vectors. In particular, the present invention relates to retroviral vectors that retain introns in genes of interest during vector production. The present invention further provides host cells and animals comprising gene delivered by the vectors. The present invention additionally provides methods of using...

20060160221 - Device and method for processing biological material: The invention relates to a device for processing biological material, which at least comprises a chamber which at least can be closed in relation to the outside and which comprises an inner space for receiving said biological material, wherein said chamber comprises at least one electrode which is placed in...

20060160222 - Modifying dna recombination and repair: The present invention provides methods of modifying in vivo mutagenesis or homeologous recombination in a eukaryote. The method of modifying in vivo mutagenesis involves transforming a eukaryote with a nucleotide sequence capable of expressing a wild-type prokaryotic MutS, MutL, MutH, MutU, NLS-MutS, NLS-MutL, NLS-MutH, NLS-MutU protein, or a combination thereof,...

20060160223 - Plasmid vectors for transformation of filamentous fungi: The invention relates to novel plasmid vectors for transformation of filamentous fungi and to a method of modifiying the genome of filamentous fungi based on these vectors. The invention furthermore relates to the modification of a specific gene via the process of homologous recombination, to recombinant expression of foreign genes...

20060154231 - Apparatus for reconstituting bone with biological fluids prior to surgical implantation: A novel apparatus and device is described for reconstituting a bone sample with a biological fluid, such as concentrated plasma or saline solutions, for example, prior to surgical implantation into a patient. In particular, the invention comprises, in certain aspects, a container in which a bone sample may be reconstituted...

20060154230 - Methods for processing biological tissue: A method for processing biological tissue used in biological prostheses includes providing a tissue procurement solution formed from a phosphate buffered saline (PBS) solution and a chelating agent. The tissue is transferred from the tissue procurement solution and undergoes chemical fixation. The fixed tissue is then immersed in a series...

20060154232 - Stack of supports, in particular for cryopreservation of biological samples: Substrates (100) are described for receiving a plurality of samples, especially for the preservation of biological samples at cryotemperatures comprising a plurality of substrate plates (11, 12, 13) arranged on top of one another as a stack (10) and comprising an anchoring axis (20) to which the substrate plates (11,...

20060154236 - Computer-assisted analysis: The present invention provides methods and systems for automated morphological analysis of cells also known as phenotypic screening. The inventive methods are particularly useful in the rapid analysis of cells required in a biological screen or in the screening for agents with a particular mechanism of action. Agents which cause...

20060154235 - Human hepatocyte-like cells and uses thereof: The present invention provides modified methods of producing human hepatocyte-like cells which exhibit phenotypes more similar to those of human hepatocytes. The present invention also provides the human hepatocyte-like cells and uses thereof. The methods of the present invention comprises a prolonged incubation period and addition of dexamethasone to the...

20060154233 - Method and device for manipulating individual small objects: A method for individually moving small objects, such as cells, from one location to another as well as a device for implementing the method is disclosed. A small object such as a cell is isolated at some initial location and moved to some destination location by the movement of the...

20060154234 - Standard: Disclosed is a reference standard for a detectable entity, the reference standard comprising a support medium, such as an embedding medium, a compact particle having a compact shape with a quantity of detectable entity coupled thereto and supported by the medium, in which the compact particle is a biological, such...

20060154243 - Antigenic peptides of sars coronavirus and uses thereof: The present invention pertains to antigenic peptides of SARS-CoV and their use in diagnostic test methods and in the treatment of condition resulting from SARS-CoV. Furthermore, this invention provides antibodies capable of specifically recognizing the peptides of the invention. The antibodies can also advantageously be used in diagnostic test methods...

20060154242 - Biomolecule chip and fabrication method thereof: Disclosed is a biomolecule chip and a fabrication method thereof The biomolecule chip of the invention includes: a substrate; an insulating layer formed on the substrate; an adhesive layer formed on the insulating layer; a seed layer formed on the adhesive layer; an opening patterned at a predetermined location within...

20060154240 - Compositions and methods related to modified retroviral vectors for restricted, site specific integration: Embodiments of the invention include compositions comprising and methods utilizing a retroviral integrase complex comprising a recombinant integrase having a domain comprising a non-native protein binding site, and a DNA binding protein comprising a DNA binding domain and a peptide binding domain that binds the non-native protein binding site of...

20060154238 - Compounds and methods of early diagnosis of cervical cancer and genital condyloma with hpv, chsp60 tumor suppressor h-ras, k-ras and pten derived peptides modified: An isolated sequence or peptide isolated from an E2, E4, E6, E7 early or late coding region of human papillomavirus (HPV) that is soluble in aqueous medium, and characterized by a linkage to another protein sequence or peptide isolated from the E2, E4, E6, E7 early or late coding region...

20060154239 - Detection of protease-resistant prion protein after asymmetric spontaneous interaction: The present invention concerns methods for detecting infectious prion protein with improved sensitivity. For this purpose the heterologous non-pathogenic protease-sensitive prion protein PrPc is added to a sample to be examined and is transformed into protease-resistant prion aggregates by asymmetric spontaneous interaction when the infectious prion protein PrPSc is present...

20060154241 - Immunochromatographic test device: An immunochromatographic test device of the present invention comprises: a sample receiving member for receiving a sample; a label holding member for holding a labeling substance to bind to an analyte contained in the sample; and a chromatographic membrane having a detection zone at which an immobilization substance to bind...

20060154237 - Soluble rna polymerase protein and methods for the use thereof: A polymerase protein originating from a eukaryotic cell and involved in the RNA silencing pathway is for the first time provided in a purified soluble form that possesses a detectable RNA polymerization activity. This polymerase is useful in methods and kits for in vitro RNA synthesis. A polymerase of the...

20060154270 - Analysis method and analysis apparatus of liquid sample: With the object of providing an analysis method and analysis apparatus of liquid sample capable of forming a temperature environment in which all reactants can react with their respective specific target substances when a liquid sample is analyzed using a solid support on the surface of which are fixed multiple...

20060154266 - Assays for tert promoter modulatory agents: Methods and compositions for assaying an agent for TERT promoter modulatory activity are provided. In the subject methods, an agent is contacted with a cell comprising a mutant telomerase structural RNA component (TR) that results in a detectable phenotype in the presence of telomerase reverse transcriptase (TERT). Also provided are...

20060154282 - Biomolecule bonding detection apparatus using rf wireless energy transmission and method thereof: An apparatus for detecting a biomolecule bonding using a radio frequency (RF) wireless energy transmission and a method thereof are disclosed. The method includes transmitting RF energy to a sensor module having one or more probe biomolecules immobilized thereon, and determining a first energy charging time of the sensor module,...

20060154255 - Blood and tissue sample assessment of mitochondrial function and biochemistry as a tool for selection for feed efficiency and other production parameters: The present invention provides methods for predicting feed efficiency in animals by correlating the association of mitochondrial function with feed efficiency. In one embodiment, the invention provides a method for determining feed efficiency comprising comparing proteins patterns and activity levels with antibody interaction of proteins associated with mitochondrial function and...

20060154251 - Carboxamide-substituted dyes for analytical applications: The present invention relates to carboxamide-substituted dyes, the production and use of such dyes as labeling groups in analytics....

20060154258 - Card-domain containing polypeptides, encoding nucleic acids, and methods of use: The invention provides caspase recruitment domain (CARD)-containing polypeptides and functional fragments thereof, encoding nucleic acid molecules, and specific antibodies. Also provided are screening methods for identifying CARD-associated polypeptides (CAPs), and for identifying agents that alter the association of a CARD-containing polypeptide with itself or with a CAP. Further provided are...

20060154268 - Circadian rhythm control gene cluster, dna chip, and methods for using the dna chip: A circadian rhythm control gee cluster (Bmal1 gene, Npas2 gene, Rev-erbα, Dbp gene, Per3 gene, Per2 gene and Per1 gene) having a given expression timing and expression sequence under normal conditions is provided along with a DNA chip having, at least, the circadian control gene group sequentially arranged. There are...

20060154269 - Cleavage of nucleic acids: The present invention relates to means for the detection and characterization of nucleic acid sequences, as well as variations in nucleic acid sequences. The present invention also relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a...

20060154277 - Compositions and methods for diagnosing or treating psoriasis: In one aspect, the present invention provides isolated nucleic acid molecules that encode a CAN-1 polypeptide, or an STG polypeptide. In another aspect, the present invention also provides isolated STG polypeptides, isolated CAN-1 polypeptides, and isolated SEEK-1 polypeptides. In another aspect, the present invention provides isolated antibodies that bind specifically...

20060154283 - Computer-directed assembly of a polynucleotide encoding a target polypeptide: The present invention outlines a novel approach to utilizing the results of genomic sequence information by computer-directed polynucleotide assembly based upon information available in databases such as the human genome database. Specifically, the present invention may be used to select, synthesize and assemble a novel, synthetic target polynucleotide sequence encoding...

20060154278 - Detection methods for disorders of the lung: The present invention is directed to prognostic and diagnostic methods to assess lung disease risk caused by airway pollutants by analyzing expression of one or more genes belonging to the airway transcriptome provided herein. Based on the finding of a so called “field defect” affecting the airways, the invention further...

20060154267 - Diagnosis and treatment of breast cancer: Methods and compositions are provided for the identification of expression signatures in ER+ breast cancer cases, where the signatures correlate with responsiveness, or lack thereof, to treatment with tamoxifen or another antiestrogen agent against breast cancer The signature profiles are identified based upon sampling of reference breast tissue samples from...

20060154259 - Diamond crystallites for biotechnological applications: A diamond-based composition that contains (1) a diamond crystallite having chemically derivatized surface groups, and (2) a polymer having functional groups, in which a portion of the functional groups bind to the chemically derivatized surface groups non-covalently. Also disclosed are methods of using such composition for analyzing a biological sample...

20060154246 - Encoded self-assembling chemical libraries (esachel): The invention concerns a chemical compound comprising a chemical moiety (p) capable of performing a binding interaction with a target molecule (e.g. a biological target) and further comprising an oligonucleotide (b) or functional analogue thereof. In a first embodiment according to the invention, the chemical compound is characterized in that...

20060154271 - Enzymatic nucleic acid treatment of diseases or conditions related to levels of ikk-gamma and pkr: The present invention relates to nucleic acid molecules, including antisense and enzymatic nucleic acid molecules, such as hammerhead ribozymes, DNAzymes, allozymes, aptamers, decoys and siRNA (RNAi), which modulate the expression or function of IKK genes, such as IKK-gamma, IKK-alpha, or IKK-beta, and PKR genes....

20060154279 - Extracellular serine protease: The present invention provides a DNA encoding a TADG-14 protein selected from the group consisting of: (a) isolated DNA which encodes a TADG-14 protein; (b) isolated DNA which hybridizes to isolated DNA of (a) above and which encodes a TADG-14 protein; and (c) isolated DNA differing from the isolated DNAs...

20060154286 - Helicase-dependent amplification of nucleic acids: Methods and a kit are provided for selectively and exponentially amplifying nucleic acids and include the use of a single strand helicase preparation or a thermostable helicase in the absence of a single strand binding protein and a DNA polymerase such that the amplification can be performed isothermally....

20060154287 - High-content screening for drugs against cancer and age-related diseases: The invention relates to methods for high-throughput screening for compounds that modulate cell growth or promoter activity. The invention provides the use of cell lines with properly regulated promoter-reporter expression, suitable for high-throughput screening. More particularly, the invention relates to such screening to identify compounds that affect cell growth and/or...

20060154262 - Identification of genes involved in metastatic progression of cancer cells: The present invention relates to the discovery, identification and characterization of an eight genes that are differentially expressed as a consequence of metastatic progression in human melanoma cells. Six of the identified metastasis elevated genes (MEG genes), encode for known proteins (MEG-1 through MEG-6). However, two of the identified genes,...

20060154294 - Interleukin-18 binding proteins, their preparation and use: Interleukin-18 binding proteins which are capable of binding IL-18 and/or modulating and/or blocking IL-18 activity are provided. Methods for their isolation and recombinant production, DNAs encoding them, DNA vectors expressing them, vecotors useful for their expression in humans and other mammals, antibodies against them are also provided....

20060154265 - Ldlr genetic markers associated with age of onset of alzheimer's disease: Haplotypes in the LDLR gene associated: with age of onset of Alzheimer's Disease are disclosed. Compositions and methods for detecting and using these LDLR haplotypes in a variety of clinical applications are disclosed. Such applications include articles of manufacture comprising compounds effective in delaying the age of onset of AD...

20060154248 - Manufacturing method and readout system for biopolymer arrays: A method for manufacturing an array of biomolecules that have complementary binding species is disclosed. The method utilize a master substrate containing a desired master array of binding molecules or ligands, e.g., DNA target molecules. A mixture of complementary, antiligand molecules, e.g., DNA probe molecules, are then hybridized to the...

20060154263 - Method and apparatus for chromosome profiling: A method and apparatus for generating an Interphase chromosome profile. The method comprises obtaining a sample containing cells having chromosomes for profiling; obtaining species specific DNA probes, wherein the DNA probes are capable of marking at least one chromosome at substantially equidistant locations on said chromosome; hybridizing the sample with...

20060154264 - Method and apparatus for parallel synthesis of chain molecules such as dna: Synthesis of chain molecules such as DNA is carried out in a conduit having an interior channel with an inlet end and an outlet end. At least one wall of the conduit is substantially transparent to selected wavelengths of light. Solid carrier particles are contained within the interior channel of...

20060154290 - Method and apparatus for pattern identification in diploid dna sequence data: This invention provides methods for identifying mutations and methylation patterns in diploid DNA sequence signal data. In particular, the invention provides methods for (1) obtaining two parental allele sequences from diploid DNA sequence signal data, (2) identifying the mutation and haplotype patterns in the two parental allele sequences, (3) assigning...

20060154280 - Method and apparatus for precise temperature cycling in chemical/biochemical processes: A method for implementing a temperature cycling operation for a biochemical sample to be reacted includes applying an infrared (IR) heating source to the sample at a first infrared wavelength selected so as to generate a first desired temperature for a first duration and produce a first desired reaction within...

20060154249 - Method and kit for detecting a risk for diabetes or a metabolic syndrome: The invention provides a method and kit for detecting or diagnosing a risk of or predisposition to diabetes or a metabolic syndrome in a subject, the method comprising the steps of providing a biological sample of the subject to be tested and detecting the presence or absence of a variant...

20060154256 - Method for covalently attaching nucleosides and/or nucleotides on surfaces and method for determining coupling yields in the synthesis of nucleotides: where L is a common suitable leaving group, the motif O—PX represents a phosphor amidite, a H-phosphonate a phosphonic acid ester, a phosphotriester, Y═O or S, N is a nucleoside or a nucleotide derivative which subsequently reacts further with a protecting group reagent and the elimination of the leaving group...

20060154245 - Method for detecting, screening and/or montoring a cancer in individual: The invention relates to a method for screening and/or detecting and/or monitoring a cancer in an individual, said method comprising determining a first parameter represented by the concentration of TIMP-1 in at least one excreta, e.g. saliva, from the individual. The invention provides a method that without the need to...

20060154289 - Method for determining metabolic flux affecting substance production: A metabolic flux affecting substance production using cells is determined by 1) creating a stoichiometric matrix based on formulas of biochemical reactions from a substrate through a desired produced substance, 2) selecting the same number of independent metabolic fluxes from all metabolic fluxes as the degree of freedom of the...

20060154261 - Method for determining susceptibility of an individual to allergen induced hypersensitivity: The present invention is related generally to a method for screening subjects to determine those subjects more likely to exhibit a hypersensitivity to allergen in the presence of airborne pollutants, such subject having a GSTM1 null genotype and/or a GSTP1 Ile/Ile genotype. This invention also provides novel or improved pharmaceutical...

20060154253 - Method for the validated construction of arrays: The present invention relates to a method for validating the synthesis of arrays, in particular of biopolymers, by step-by-step construction from protected and labeled synthesis building blocks....

20060154284 - Method of isolating nucleic acid using material positively charged at first ph and containing amino group and carboxyl group: A method of isolating nucleic acid from a sample containing nucleic acid is provided. The method includes contacting the sample with a bifunctional material that contains an amino group and a carboxyl group and is positively charged at a first pH to allow binding of the nucleic acid to the...

20060154252 - Methods and composition for identifying therapeutic agents of atherosclerotic plaque lesions: The present invention relates to a method for identifying therapeutic agents for reducing and monitoring the growth, erosion, rupture or stability of an atherosclerotic plaque comprising the analysis of the differential expression of at least two genes coding proteins chosen among among Stearoyl CoA desaturase, Phosphatidic acid phosphate, and Phosphoinositide-specific-phospholipase-B1,...

20060154288 - Methods for analyzing nucleic acid sequences: The present invention is directed to a method of sequencing a target nucleic acid molecule having a plurality of bases. In its principle, the temporal order of base additions during the polymerization reaction is measured on a molecule of nucleic acid, i.e. the activity of a nucleic acid polymerizing enzyme...

20060154276 - Methods of diagnosing inflammatory bowel disease: The present invention provides methods for diagnosing inflammatory bowel disease (IBD) or for differentiating between Crohn's disease (CD), ulcerative colitis (UC), and indeterminate colitis (IC) in an individual by using a combination of learning statistical classifiers based upon the presence or level of one or more IBD markers in a...

20060154247 - Methods,compositions and kits for cell separation: Methods, compositions and kits for concentrating or separating cells containing target nucleic acid are disclosed, especially m mixtures containing the cells and other components such as impurities. The methods can keep a large proportion of the cells intact, allowing the cells to be employed after separation (e.g. cultured) and/or which...

20060154292 - Novel 16srrna gene data and probes useful for detecting and quantitating microorganism from natural environment: An isolated 16S rDNA having a nucleotide sequence selected from SEQ ID NOs: 1, 2, 3, and 4. The invention also relates to an RNA or DNA probe with a length ranging from 10 to 50 bases which comprises at least one nucleotide sequence selected from SEQ ID NOs: 1,...

20060154272 - Novel coding sequence haplotypes of the human brca2 gene: Five novel DNA and protein sequences have been determined for the BRCA2 gene, as have been ten polymorphic sites and their rates of occurrence in the normal alleles of BRCA2. The sequences BRCA2(omi 1-5) and the ten polymorphic sites will provide greater accuracy and reliability for genetic testing. One skilled...

20060154250 - Novel compositions and methods in cancer: The present invention relates to novel sequences for use in detection, diagnosis and treatment of cancers, especially lymphomas. The invention provides cancer-associated (CA) polynucleotide sequences whose expression is associated with cancer. The present invention provides CA polypeptides associated with cancer and provides diagnostic compositions and methods for the detection of...

20060154244 - Novel g-protein coupled receptor: HNHLA17 polypeptides and polynucleotides and methods for producing such polypeptides by recombinant techniques are disclosed. Also disclosed are methods for utilizing HNHLA17 polypeptides and polynucleotides in diagnostic assays....

20060154295 - Nucleotide sequence which encodes a flavin monooxygenase, the corresponding protein and their uses in the spheres of diagnosis and therapy: The present invention concerns, in particular, human flavin-containing monooxygenase 2 (hFMO2), and another human enzyme of the FMO, hFMOx family, their nucleotide and polypeptide sequences. The present invention also concerns vectors for cloning and/or expression containing said nucleotide sequences and cells transformed by these vectors and method for preparing said...

20060154281 - Reaction chamber: The invention describes novel reaction chambers that include a case with at least one opening and a flat bottom flange attached to the first side of a substrate with at least one microarray of materials attached thereto. The case and the substrate are attached through an adhesive layer with at...

20060154291 - Reagents and methods useful for detecting diseases of the breast: A set of contiguous and partially overlapping cDNA sequences and polypeptides encoded thereby, designated as BS322 and transcribed from breast tissue, is described. These sequences are useful for the detecting, diagnosing, staging, monitoring, prognosticating, in vivo imaging, preventing or treating, or determining the predisposition of an individual to diseases and...

20060154275 - Regulated genes in cervical cancer: Polynucleotides, as well as polypeptides encoded thereby, that are differentially expressed in SCCC cells are provided. The polynucleotides find use in diagnosis of cancer, and classification of cancer cells according to expression profiles. The methods are useful for detecting cervical cancer cells, facilitating diagnosis of cervical cancer and the severity...

20060154293 - Regulation of human prostasin-like serine protease: Reagents which regulate human prostasin-like enzyme activity and reagents which bind to human prostasin-like enzyme gene products can be used to regulate human prostasin-like enzyme activity. Such regulation is particularly useful for treating metastasis of malignant cells, tumor angiogenesis, inflammation, atherosclerosis, neurodegenerative diseases, and pathogenic infections....

20060154254 - Rotaxane compound, rotaxane-bonded solid substrate, and biochip using the same: The present disclosure provides a rotaxane compound that can be used to separate molecules within a linkage layer formed on a solid substrate of a biochip by a predetermined distance. The compound is represented by Formula 1, in which a compound of Formula 3 vertically passes through a cavity of...

20060154260 - Sample preparation methods for diagnostic analyses: A method for determining the presence or amount of an analyte in a food sample is provided. The method includes the steps of contacting a food sample containing polymer or lipid with an enzyme capable of degrading the polymer or lipid, and analyzing at least a portion of the enzyme-treated...

20060154257 - Screening method for identification of efficient pre-trans-splicing molecules: The present invention provides methods and compositions for rapid high capacity functional screening to identify optimal pre-trans-splicing molecules (PTMs). The compositions of the invention include PTM expression libraries capable of encoding candidate PTMs designed to interact with a target precursor messenger RNA molecule (target pre-mRNA) and mediate a trans-splicing reaction...

20060154273 - System and computer software products for comparative gene expression analysis: Methods and computer software products are provided for analyzing gene expression data. In one embodiment, methods, systems and computer software are provided for comparative gene expression analysis using intensity dependent normalization factors....

20060154274 - Topographic genotyping: The present invention pertains to a method for topographic genotyping. The method comprises the steps of placing a biological specimen having DNA of a patient under a microscope. Then there is the step of inspecting the biological specimen microscopically with the microscope. Next there is the step of choosing a...

20060154285 - Zebrafish heterotrimer g-protein gamma 2 subunit (gng2): The invention provides sequences of the zebrafish heterotrimeric G-protein gamma 2 subunit (GNG2). The invention also provides methods of inhibiting and promoting GNG2-dependent angiogenesis in vertebrates, particular mammals, including humans, for the treatment of angiogenesis-related diseases. The invention also provides methods of identifying compounds that promote or inhibit angiogenesis through...

20060154300 - Biomolecule bonding detection apparatus using wireless antenna and method thereof: Disclosed is a biomolecule binding detection apparatus and a method thereof, which detects biomolecule bonding by measuring change in characteristics of a test signal transmitted by an antenna on which probe biomolecules are immobilized. The biomolecule bonding detection apparatus includes a transmitting unit on which probe biomolecules are immobilized, the...

20060154304 - Clinical applications of crystalline diamond particles: A method for profiling proteins in a biological sample by mixing the sample and a diamond-based composition. The diamond-based composition includes a diamond crystallite having a surface that contains chemically derivatized surface groups and a polymer having a plurality of functional groups non-covalently bound to the surface groups. Proteins in...

20060154305 - Combinatorial synthesis of ceramic materials: A combinatorial library includes a gelcast substrate defining a plurality of cavities in at least one surface thereof; and a plurality of gelcast test materials in the cavities, at least two of the test materials differing from the substrate in at least one compositional characteristic, the two test materials differing...

20060154303 - Evanescent wave excited fluorescence detection method: In the case where many interactional substances are immobilized on the front surface side of a light transmitting plate capable of generating evanescent waves on the front surface, and where the fluorescence levels in the interactions between the interactional substances and a fluorescence labeled test substance are detected in a...

20060154302 - Iterative, subtractive immunoaffinity method for proteome analyte enrichment: An immunoaffinity method according an embodiment of the present invention is described for selectably removing highly abundant proteins (HAP) from a sample which contains a mixture of HAP and lower abundance proteins (LAP). One method includes providing a sample having a concentration of original HAP and LAP therein and producing...

20060154297 - Marker substances and the use of the same in diagnostic methods: The invention relates to a diagnostic method and to marker substances which enable manipulations in endogenic marking to be detected and thus prevented. According to the invention, a metabolisable substance is added to non-metabolisable marker substances, said metabolisable substance revealing a manipulation when detected in excreta....

20060154298 - Method of synthesis and testing of combinatorial libraries using microcapsules: The invention describes a method for the synthesis of compounds comprising the steps of: (a) compartmentalizing two or more sets of primary compounds into microcapsules; such that a proportion of the microcapsules contains two or more compounds; and (b) forming secondary compounds in the microcapsules by chemical reactions between primary...

20060154296 - Pigment protein: An object of the present invention is to provide a novel chromoprotein derived from Cnidopus japonicus. The present invention provides a chromoprotein derived from Cnidopus japonicus having the following properties: (1) the absorption maximum wavelength is 559 nm and the fluorescence maximum wavelength is of 578 nm; (2) the molar...

20060154299 - Protein microarray device having internal calibrators and methods of using therefor: The present invention relates to protein microarray devices having an internal calibrator, kits containing such devices, and methods of using such devices. Such a device comprises a plurality of protein arraying pads on a support substrate, a plurality of protein arraying spots on each pad, and a calibrator disposed on...

20060154301 - Test kit for immunochromatography: A test kit for immunochromatography comprising a test strip and a test tube is described. The test strip comprises a detection zone for detecting an analyte in a sample. The test tube accommodates the test strip. The test tube comprises an indicator at a position corresponding to the detection zone...

20060154306 - Methods detecting, characterising and monitoring hydrocarbon reservoirs: The present invention relates to a method of detecting, characterizing or monitoring a hydrocarbon source, which method comprises the genotypic analysis of a sample for the presence of one or more thermophilic or extemophilic microorganisms and in particular to the generation of microbiological profiles of samples and to the comparison...

20060154307 - Receptor assay: The present invention relates to receptor/reporter fusion protein based assays for detecting an effect test compounds have on a particular membrane receptor, as well as to receptor/reporter fusion proteins for use in such assays and compounds identified by the assays as having interesting/useful effects. Suitable membrane receptors are growth factor...

20060154308 - Buffy coat tube and float system and method: A tube and float system for use in separation and axial expansion of the buffy coat is provided. The system includes a transparent, or semi-transparent, flexible sample tube and a rigid separator float having a specific gravity intermediate that of red blood cells and plasma. The sample tube has an...

20060154310 - Diagnosis and therapy of cancer using sgp28-related molecules: The present invention relates to methods and compositions for the diagnosis and therapy of prostate cancer which utilize isolated polynucleotides corresponding to the human SGP28 gene, proteins encoded by the SGP28 gene and fragments thereof, and antibodies capable of specifically recognizing and binding to SGP28 proteins....

20060154313 - Human b7 polypeptide b7-h3a: This invention relates to B7-H3A, a new member of the human B7 polypeptide family, methods of making such polypeptides, and to methods of using them to treat immunological conditions and to identify compounds that alter B7-H3A polypeptide activities....

20060154309 - Method of examining cancer cells and reagent therefor: A method for examining cancer cells by which examination of cancer cells may be carried out simply and efficiently without using an expensive apparatus, and a reagent therefor are disclosed. In the method for examining cancer cells according to the present invention, cancer cells separated from the body, which cells...

20060154311 - Novel bladder matrix protein peptides and methods of detection of bladder cancer: Nuclear matrix proteins (NMP) which are characterized by a defined expression in tissue are provided. These NMPs are useful markers in diagnosing and monitoring the stage of malignancy of a cell and treating cell proliferative disorders associated with the NMP. Also provided are substantially purified polypeptides and polynucleotide sequences encoding...

20060154312 - Tubulin isotype screening in cancer therapy using halichondrin b analogs: Chemotherapeutic agents that interfere with microtubule assembly or disassembly in the cell are potent inhibitors of cell replication. Examples of such agents include halichondrin B analogs. It has been shown that the susceptibility of certain cancers to analogs of halichondrin B correlates with the expression of particular tubulin isotypes or...

20060154314 - Gfp-snap25 fluorescence release assay for botulinum neurotoxin protease activity: The present invention provides a nucleic acid molecule which contains a nucleotide sequence encoding a SNAP-25 substrate which includes (i) a green fluorescent protein; (ii) a first partner of an affinity couple; and (iii) a portion of SNAP-25 that includes a BoNT/A, BoNT/C1 or BoNT/E recognition sequence containing a cleavage...

20060154315 - Method of use of one step immunochromatographic device for streptococcus a antigen: A method to determine the presence or absence of Streptococcus Group A antigen in a sample, comprising the following steps: extracting the antigen from the sample in an assay chamber with two or less extraction reagents, wherein the two reagents may be added to the assay chamber in no particular...

20060154316 - Elisa method for the detection of guanylate binding protein 1 (gbp-1): A method for the identification and/or quantification of GBP-1 or fragments of this protein in the culture supernatant of a tissue sample, a body fluid sample or a sample from a cell culture supernatant is described....

20060154320 - Enzyme activity measurement using bio-layer interferometry: Disclosed are enzyme assays using biolayer interferometry. Assays may be carried out using immobilized substrate or with a substrate capture format. In certain embodiments, the assays are carried out using unlabeled substrates. The methods are broadly applicable to enzyme assay measurements, can be carried out in vivo or in vitro,...

20060154319 - Inhibition of bradykinin release: The invention is related to a method for treating or preventing a disorder resulting from the release of bradykinin in said mammal, particularly a mammal which produces a HBP which binds to a HBP antagonist, e.g. a monoclonal antibody that binds to at least one epitope of human HBP, comprising...

20060154317 - Method of immunoassaying taritrate resistant acid phosphatase 5b and kit to be used therein: Specific and accurate immunoassay of tartrate resistant acid phosphatase 5b (TRACP 5b) in a specimen may be carried out by bonding TRACP 5b in the specimen to an antibody, subjecting the TRACP 5b bonded to the antibody to enzyme reaction with a 2-halo-4-nitrophenylphosphoric acid or a salt thereof, a substrate...

20060154321 - Polyclonal-monoclonal elisa assay for detecting n-terminus probnp: A specific and sensitive in vitro ELISA assay and diagnostic test kit is disclosed for determining levels of NT-proBNP protein in a variety of bodily fluids, non-limiting examples of which are blood, serum, plasma, urine and the like. The NT-proBNP ELISA assay test employs the sandwich ELISA technique to measure...

20060154318 - Stable isotope labeled polypeptide standards for protein quantitation: This invention relates to proteins having an amino acid sequence containing several amino acid subsequences found in nature and wherein at least two different subsequences act as monitor sequences, said subsequences being part of at least one natural protein which is a target protein, wherein the end of each of...

20060154322 - Methods and systems for determining and controlling glycemic responses: The present invention provides a method of determining a standard comestible Equivalent Glycemic Load of a dietary comestible comprising: (a) establishing a reliable glycemic response index for a standard comestible, wherein the index correlates glycemic response with glycemic load; (b) determining the glycemic response produced by a dietary comestible, and...

20060154323 - Method for obtaining dynamic and structural data pertaining to proteins and protein/ligand complexes: This invention provides an NMR method for obtaining both entropic and enthalpic data on proteins and protein/ligand complexes which can be used to obtain accurate structural and dynamic data of proteins and protein complexes having a wide range of molecular weights. An embodiment of the invention provides proteins which contain...

20060154324 - Prostate-specific antigen probes for optical imaging: This invention provides a novel probe, which contains a prostate peptide substrate. The probe can be used as a prostate diagnostic agent for optical imaging. An 11 amino acid peptide substrate is synthesized for specific prostate-specific antigen (PSA). This protected graft copolymer (PGC) consists of a poly-L-lysine (PL) backbone and...

20060154325 - Synthesis of epoxide based inhibitors of cysteine proteases: Epoxide based cysteine protease inhibitors containing peptide derivatives and methods for synthesizing them in sold phase are disclosed. Preferably, an epoxy succinyl “warhead” (for binding to the enzyme) is prepared, having two amino acid residues or residue-like structures on either side. Natural and non-natural amino acids may be used. The...

20060154326 - Metabolic biosensor and uses thereof: The present invention relates to a device for analyzing the metabolism of cells involved in a culture or fermentation process. A sample of the culture or fermentation medium is submitted to at least one oxidation-reduction reaction. The device of the invention includes two electrodes that measures the electric conductivity of...

20060154327 - System and method for determining fill volume in a container: A system and method for detecting a pathogen in a sample is provided, the system capable of measuring the volume of a sample in a container through the use of various measurement technologies, thereby ensuring that a user is aware of volumes not meeting specification and/or allowing correction of results...

20060154328 - Ionic liquids: This invention relates to ionic liquids and their use as solvents in biocatalysis. According to a first aspect of the invention there is provided a method of carrying out an enzyme-catalysed reaction comprising providing a liquid reaction medium which comprises an ionic liquid including an ion which comprises a functional...

20060154330 - Akap18 delta, a novel splicing variant of a protein kinase a anchor protein and the use of the same: The invention relates to a nucleic acid sequence encoding a protein kinase A anchor portein, to the use of said nucleic acid sequence in a fusion protein, to a method of determining the interaction of said protein kinase A anchor protein with regulatory subunits of protein kinase A, and to...

20060154331 - Erythrocyte differentiation factor, gene encoding same, and methods of use thereof: The present invention relates to an erythrocyte differentiation factor designed Codanin-1, and to an isolated nucleic acid which encodes for the differentiation factor. Mutations in the differentiation factor are associated with Congenital Dyserythropoietic Anemias (CDA), a group of inherited red blood cell disorders associated with dysplastic changes in late erythroid...

20060154329 - Expression system: The present invention provides a recombinant polynucleotide, the polynucleotide comprising a first and a second sequence, the first sequence encoding a signal peptide comprising a TAT signal and a Sec avoidance signal and the second sequence encoding a heterologous protein. The sequence of the signal peptide is M-X1—K/R—X2—K/R—X3—RR—X4—K/R-A in which...

20060154337 - Gene encoding chondroitinase abc and uses therefor: Nucleic acid sequences coding for the chondroitinase ABC gene and isolated chondroitinase ABC protein produced in a host cell transformed with a nucleic acid vector directing the expression of a nucleotide sequence coding for chondroitinase ABC protein are described. Chondroitinase ABC prepared by chemical synthesis is also described. Monoclonal and...

20060154334 - Method of producing an antibody to epidermal growth factor receptor: The present invention is directed to a method of producing an antibody to Epidermal Growth Factor Receptor (EGFR). The method includes producing transformed cells that express EGFR antibodies, culturing the transformed cells, harvesting the transformed cells to collect the EGFR antibodies, and purifying the EGFR antibodies....

20060154338 - Novel antibiotic alternatives: The present invention relates to two recombinant colicin expression systems, one utilizing a yeast expression system that produces a protein that is inexpensive to purify, and the other utilizing a plasmid expression system to be used as a probiotic culture. The recombinant colicins provide effective alternatives to conventional antibiotics and...

20060154339 - Novel clock gene bmal2: The present invention provides novel clock proteins BMAL2 (Brain-Muscle-Arnt-Like protein2), which is crucial for the clock oscillation mechanism including photic-input pathway and output pathway, novel clock genes encoding the proteins, a screening method using the proteins to screen a promoter or a suppressor of the promoter transactivation, and the like....

20060154335 - Novel members of the steroid/thyroid superfamily and uses therefor: Novel peroxisome proliferators-activated receptor subunits designated PPARγ and PPARδ are described. Nucleic acid sequences encoding the receptor subunits, expression vectors containing such sequences and host cells transformed with such vectors are also disclosed, as are heterodimeric PPAR receptors comprising at least one of the invention subunits, and methods for the...

20060154336 - Nucleic acid molecules for optimized cab-2 expression and their use: The invention provides for nucleic acid molecules encoding a CAB-2 protein and variants thereof. The invention also provides constructs, transformed eukaryotic cells, and methods of producing CAB-2 protein and variants thereof....

20060154333 - Pan-her antagonists and methods of use: The present invention features human epidermal receptor (HER) antagonists. These antagonists are polypeptide variants of ligands of HER. The HER ligand polypeptide variants of the invention possess Pan-HER antagonistic properties and can inhibit at least one HER-mediated biological activity of one or more HER subtypes, such as inhibition of the...

20060154332 - Selective capture and enrichment of proteins expressed on the cell surface: The present invention provides a method for enriching cell surface proteins by oxidizing glycosylated protein with periodate. The present invention further provides that the cell surface proteins are obtained from intact cells either from cell lines or from tissues. The present invention provides a method not only capable of obtaining...

20060154340 - Expression of potato proteinase inhibitor ii in microbial hosts: Multiple microbial host genetic systems with various molecular constructs enabling subcellular targeting, fusion protein generation and co-expression of ancillary enzymatic activities are used in the expression of potato proteinase inhibitor II. The expression levels achieved exceed previous reports by up to 10 to 1000 fold....

20060154341 - Sample processing vessels: A sample vessel for holding a sample may include a sample containing portion having at least one pressure gate disposed within the sample containing portion to divide the sample containing portion into a plurality of segments. At least one segment of the sample containing portion may have a wall constructed...

20060154342 - Acid fungal proteases: The present invention is directed to novel acid proteases and more specifically to NSP24 family proteases and NSP25 family proteases including biologically active fragments thereof and to nucleic acid molecules encoding said proteases. Also provided are vectors and host cells including nucleic acid sequences coding for the proteases, methods for...

20060154343 - Method for producing monatin: A solution to be treated containing monatin and impurities is treated with a nonpolar resin having aromatic rings, whereby monatin is separated from the solution. Treatment with the nonpolar resin having aromatic rings results in efficient separation of monatin under a mild pH condition....

20060154345 - Metabolic engineering of amino acid production: The present invention is directed towards the fermentative production of amino acids, providing microorganisms, methods and processes useful therefor. Microorganisms of the invention are capable of converting glucose to amino acids other than L-isoleucine, L-leucine and L-valine with greater efficiency than the parent strain. The efficiency of conversion may be...

20060154344 - Method for producing l-lysine or l-threonine: A bacterium belonging to the genus Escherichia which has an ability to produce L-lysine or L-threonine and which is modified so that a malic enzyme does not function normally in a cell, and a method for producing L-lysine or L-threonine, comprising culturing the bacterium in a medium to produce and...

20060154346 - Microorganism producing 5'xanthylic acid: The invention relates to Corynebacterium ammoniagenes CJXOL 0201 KCCM 10447 producing 5′-xanthylic acid. More specifically, the invention relates to Corynebacterium ammoniagenes CJXOL 0201 KCCM 10447 which is a mutant strain of Corynebacterium ammoniagenes KCCM 10340 having a resistance to oligomycin. In order to obtain mutant strain having enhanced respiratory activity,...

20060154347 - Process for the manufacture of spiroketals: The present invention is directed to the use of yeasts to produce spiroketal fragrance materials. The preferred yeasts used in the method of the invention are Bensingtonia ciliate and Cryptococcus laurentii....

20060154348 - Process for producing hmg-coa reductase inhibitors: e

20060154349 - Production of coenzyme q-10: Improved process for the preparation of CoQ10 by fermentation of microorganisms of the genus Rhodobacter transformed with the mevalonate operon of Paracoccus zeaxanthinifaciens....

20060154350 - Method of production of biodegradable lactic acid polymers and the use of lactic acid polymers produced using such a method: The invention relates to the use of lactic acid and its esters produced upon microbiological fermentation of organic substances, particularly cereal starch, for producing biodegradable lactic acid polymers and use of the biodegradable polymer produced using such a technique. The areas of application of the invention include food processing and...

20060154351 - Gene participating in acetic acid tolerance, acetic acid bacteria bred using the gene, and process for producing vinegar with the use of the acetic acid bacterium: The present invention is intended to provide novel genes participating in acetic acid tolerance of acetic acid bacteria, and a method of improving acetic acid tolerance of microorganisms, particularly that of acetic acid bacteria by using the genes, further a method of efficiently producing vinegar with acetic acid at higher...

20060154353 - Acid fungal protease in fermentation of insoluble starch substrates: The invention is directed to methods of producing ethanol and decreasing residual starch production in a no cook fermentation comprising contacting granular starch containing substrates with a granular starch hydrolyzing enzyme, a protease, and a fermenting microorganism under suitable fermentation conditions at a temperature below the starch gelatinization temperature of...

20060154352 - Upflow settling reactor for enzymatic hydrolysis of cellulose: A process for the enzymatic hydrolysis of cellulose to produce a hydrolysis product from a pre-treated cellulosic feedstock is provided. The process comprises introducing an aqueous slurry of the pre-treated cellulosic feedstock at the bottom of a hydrolysis reactor. Axial dispersion in the reactor is limited by avoiding mixing and...

20060154354 - High yield pertussis vaccine production strain and method for making same: The present invention provides a vaccine production strain of Bordetella bronchiseptica that produces a pertussis toxin in high yield. The present invention provides a method for creating a Bordetella bronchiseptica cell line which produces a Bordetella pertussis toxin comprising the steps of introducing a plasmid containing a DNA encoding antibiotic...

20060154355 - Method for constructing transgenic economic seaweeds: This invention relates to marine algae, and more particularly, to a method for producing improved seaweed strains by genetic engineering. The vector for transformation were constructed by inserting the high-plant or algae-derived promoters upstream of foreign reporter genes or such cassettes that functional genes are fused with antibiotics or herbicide-resistant...

20060154356 - Method for treating organic matter to promote mouldering: The present invention relates to a method for treating organic matter to promote mouldering wherein the organic material is subjected to a splitting process followed by a freeze drying process prior to being transferred for deposition for mouldering....

20060154357 - Systems and methods for ex-vivo organ care: The invention provides, in various embodiments, systems, devices and methods relating to ex-vivo organ care. In certain embodiments, the invention relates to maintaining an organ ex-vivo at near-physiologic conditions....

20060154358 - Systems and methods for ex-vivo organ care: The invention provides, in various embodiments, systems, devices and methods relating to ex-vivo organ care. In certain embodiments, the invention relates to maintaining an organ ex-vivo at near-physiologic conditions....

20060154359 - Systems and methods for ex-vivo organ care: The invention provides, in various embodiments, systems, devices and methods relating to ex-vivo organ care. In certain embodiments, the invention relates to maintaining an organ ex-vivo at near-physiologic conditions....

20060154360 - Hybridization system using the control of pump and valves in closed system: Provided is a hybridization system for hybridizing a biochip including: a chamber device including at least a hybridization chamber including a support for a biochip and a first cover having a sample inlet; an agitation device including: two air channels connected to ends of the hybridization chamber; two valves disposed...

20060154361 - Bioreactors with substance injection capacity: A bioreactor with substance injection capability. In one embodiment, the bioreactor includes a first substrate having a first surface, an opposite second surface and edges. The bioreactor further includes a second substrate having a first surface and an opposite second surface, defining a cavity with a bottom surface, where the...

20060154362 - Garden composter: In accordance with this invention, there is provided a garden composter for decomposing garden waste in which the composter has a longitudinally extending cylindrical drum which is mounted to a support frame which is adapted to space the drum from a supporting surface. Tumbling means are provided for coupling the...

20060154363 - Proliferation and delivery apparatus: A portable, disposable, unitary microorganism proliferation and delivery apparatus (10) is provided. The apparatus (10) comprises a proliferation chamber (12) for containing a growth medium (14); and an inoculation chamber (16) for containing an inoculum (18). The proliferation and inoculation chambers (12 and 16) are connected by a tube (19)...

20060154364 - Stem cell cultivation devices and methods: The present invention relates to a device and methods for culturing stem cells, and in particular, for culturing ocular stem cells and the use of stem cells cultured using the devices and methods of the invention for the treatment of diseases....

20060154367 - Cartilage and bone repair and regeneration using postpartum-derived cells: Cells derived from postpartum tissue and methods for their isolation and induction to differentiate to cells of a chondrogenic or osteogenic phenotype are provided by the invention. The invention further provides cultures and compositions of the postpartum-derived cells and products related thereto. The postpartum-derived cells of the invention and products...

20060154365 - Cultured three dimensional tissues and uses thereof: The present disclosure provides compositions of three dimensional tissue that can be administered into tissues and organs using minimally invasive methods. The three dimensional tissues elaborate a repertoire of growth factors that facilitate repair or regeneration of damaged tissues and organs....

20060154366 - Treatment of osteochondral diseases using postpartum-derived cells and products thereof: Cells derived from postpartum tissue and methods for their isolation and induction to differentiate to cells of a chondrogenic or osteogenic phenotype are provided by the invention. The invention further provides cultures and compositions of the postpartum-derived cells and products such as lysates related thereto. The postpartum-derived cells of the...

20060154368 - Transgenic mice containing cx2 gene disruptions: The present invention relates to transgenic animals, as well as compositions and methods relating to the characterization of gene function. Specifically, the present invention provides transgenic mice comprising mutations in a CX2 gene. Such transgenic mice are useful as models for disease and for identifying agents that modulate gene expression...

20060154370 - Efficient gene suppression using a transfer rna promoter in herpes virus vectors to deliver small interference rnas: The invention provides herpes virus nucleic acid vectors for expressing shRNAs in mammalian cells and thereby silencing target genes. The vectors include (a) a herpes virus packaging signal sequence; (b) a herpes virus origin of replication; (c) a segment expressing a light-emitting marker; and (d) a transfer RNA promoter upstream...

20060154369 - Promoter sequences from wssv immediate early genes and their uses in recombinant dna techniques: Disclosed herein are isolated promoter-regulatory regions from a newly identified WSSV immediate early (IE) gene, ie1 (immediate early gene #1), which exhibit promoter activity to drive the transcription of a target gene in non-native host cells. The isolated promoter-regulatory regions can be used in the construction of a variety of...

20060147894 - Jacketed vessel for holding semen for sex biasing mammals through artificial insemination and systems and methods for enhancing the probability of sex biasing using the same: A jacketed container for holding a semen sample from a mammalian donor at a predetermined temperature for a predetermined period of time prior to incubation to enhance the probability of obtaining offspring of a selected sex through artificial insemination is disclosed. In one embodiment, the semen sample is collected and...

20060147895 - System, chamber, and method for fractionation, elutriation, and decontamination of fluids containing cellular components: A chamber, system, and method for separating a selected component from a fluid are provided. The chamber is capable of rotating about the central axis of a centrifuge device and includes a radially-extending duct having an optimized variable cross-sectional area that decreases in relation to the outward radial distance from...

20060147898 - Cell microchip: A cell microchip with which various measurements of the action of medicaments, etc. by the use of biological reaction using a small amount of cells can be accomplished simply within a brief period of time; and a method of assay with the use of the cell microchip. In particular, there...

20060147897 - Characterization of arteriosclerosis by optical imaging: A method and system for detecting abnormalities in the properties of the walls of a subject's blood vessels by observing the characteristics of blood flow in vessels which are optically accessible, such as the retinal vasculature. A time sequenced series of images is taken, and the images are processed to...

20060147900 - Compositions and methods for determining oocyte development potential: The present invention relates to the field of reproduction (e.g., human reproduction). In particular, the present invention provides markers (e.g., pentraxin 3 and Annexin A6), and methods of using the same to determine oocyte development potential. The present invention also provides compositions and methods for modifying oocyte development potential and...

20060147901 - Devices and methods to image objects by time delay integration: Devices and methods for automated collection and image analysis are disclosed that enable identification or classification of microscopic objects aligned or deposited on surfaces. Such objects, e.g. detectably labeled rare target cells, are magnetically or non-magnetically immobilized and subjected to Time Delay Integration imaging (TDI). Incorporation of TDI technology into...

20060147903 - In vitro organ modeling with cell culture tool: A method of culturing different cell types from a single organ in a cell culture tool having more than one well includes depositing the different cell types from the single organ in separate wells, culturing the different cell types in the separate wells and filling the cell culture tool to...

20060147896 - Method and device for manipulating samples: The invention relates to a method and a device for manipulating samples, in particular tissue samples, with at least one needle (3) for punching samples from preparations (5), in particular prepared tissue specimen, at defined positions and a control device (13) to control the needle (3). The aim of the...

20060147899 - Multicellular metabolic models and methods: The invention provides a computer readable medium or media, having: (a) a first data structure relating a plurality of reactants to a plurality of reactions from a first cell, each of said reactions comprising a reactant identified as a substrate of the reaction, a reactant identified as a product of...

20060147904 - Use of resveratrol to regulate expression of apolipoprotein a1: Described are new methods for promoting the expression of apolipoprotein A1 (APO A1) for increasing levels of HDL, and assays for screening and identifying compounds for regulating expression of the APO A1 protein....

20060147902 - Yeast screens for treatment of human disease: Screening methods for identifying substances that provide therapeutic value for various diseases associated with protein misfolding are provided. Genetic and chemical screening methods are provided using a yeast system. The methods of the invention provide a rapid and cost-effective method to screen for compounds that prevent protein misfolding and/or protein...

20060147906 - Anti-hpv-16 e7 antibodies and their use: The present invention relates to an anti-HPV-16 E7 antibody obtainable by (a) eliciting an in vivo humoral response against highly purified HPV-16 E7 protein or a fragment thereof in a non-human vertebrate; and (b) affinity-purifying antibodies as obtained in the eliciting-step (a). Furthermore, the invention provides for the use of...

20060147905 - Method for the specific identification of orthopoxvirus with the aid of a miniature biological chip: The invention relates to an alternative express-method for specific identification of orthopoxviruses with the aid of microchips by hybridising DNA-DNA. Said method involves a two-stage PCR producing a single stained fluorescently labelled DNA fragment, a hybridisation on a biochip containing an original set of typing oligonucleotides and original procedures for...

20060147908 - \"prionins\", highly specific markers for noninvasive presymptomatic detection of tse diseases, and targets for therapeutic reagents to prevent and control tse diseases in animals and humans.: Proteins expressed from within the prion protein genes of all animals and humans, “prionins”, against which reagents can be prepared for accurate pre-symptomatic diagnosis, for detecting latent TSE, for detecting TSE contamination of food, blood and blood products and for therapeutic treatment of Bovine spongiform encephalopathy (BSE) in cows, Scrapie...

20060147982 - 32 human secreted proteins: The present invention relates to novel human secreted proteins and isolated nucleic acids containing the coding regions of the genes encoding such proteins. Also provided are vectors, host cells, antibodies, and recombinant methods for producing human secreted proteins. The invention further relates to diagnostic and therapeutic methods useful for diagnosing...

20060147947 - Ampk pathway components: Control of AMPK activity enables interventions that modulate lifespan regulation. Agents which alter lifespan regulation of a cell or organism are identified by screening for compounds that regulate AMPK activity or AMPK pathway activity. The agents so-identified and compounds known to alter AMPK pathway activity can be administered to a...

20060147930 - Amplified and overexpressed gene in colorectal cancers: This invention relates to methods to diagnose colon cancer and other proliferative diseases by detecting amplification of genes and gene products on chromosome 20q13.2, a region whose amplification is associated with a poor cancer prognosis....

20060147919 - Analysis of biological samples: The invention provides a method of determining the degree of similarity between gene expression in a biological sample of interest and that in individual reference samples by use of: i) a nucleic acid probe library representative of a pattern of gene expression in the biological sample of interest; and ii)...

20060147972 - Apparatus for detecting biomolecular bonding and a method thereof: An apparatus and method for detecting biomolecular bonding are disclosed. The apparatus comprises a substrate; a rotation unit mounted on the substrate, a probe biomolecule immobilized on a surface of the rotation unit; an input port and an output port connected with the substrate. A frequency detector is connected with...

20060147968 - Biochip substrate used for immobilizing biomaterials: The present invention provides a biochip substrate used for immobilizing biomaterials, which has a matrix and at least one metal oxide thread-like nano-object formed vertically on a surface of the matrix. The metal oxide thread-like nano-object is employed for immobilizing the biomaterial onto the matrix of the chip substrate directly,...

20060147970 - Biomolecule detector and detection method using the same: Disclosed is a biomolecule detector and a detection method using the same. The biomolecule detector includes a biomolecule chip having a substrate and a slit formed therein. Biomolecules are immobilized on at least a portion of the edges of the slit. A light source directs incident light toward the biomolecule...

20060147987 - Bivalent binding molecules of 7 transmembrane g protein-coupled receptors: Described herein are methods for identifying and preparing bivalent binding molecules to 7 transmembrane G protein-coupled receptors. The methods disclosed herein are based on the SELEX method for generating high affinity nucleic acid ligands. SELEX is an acronym for Systematic Evolution of Ligands by EXponential enrichment. The methods of this...

20060147938 - Cftr allele detection assays: The present invention provides compositions and methods for the detection and characterization of mutations associated with cystic fibrosis. More particularly, the present invention provides compositions, methods and kits for using invasive cleavage structure assays (e.g. the INVADER assay) to screen nucleic acid samples, e.g., from patients, for the presence of...

20060147931 - Characterization of hupb gene encoding histone like protein of mycobacterium tuberculosis: Oligonucleotide primers for specific amplification of the hupB gene of Mycobacterium species selected from the group consisting of Seq ID No. 1, Seq ID No. 2, Seq ID No. 3, Seq ID No. 4, Seq ID No. 5 and a method for differentiating Mycobacterium species based on target gene encoding...

20060147940 - Combinatorial affinity selection: In one aspect of the invention, methods for analyzing nucleic acid sample are provided. In a preferred embodiment, nucleic acids are selected using affinity matrices prior hybridization with a microarray. In another preferred embodiment, multivalency nucleic acid probe structures are used for affinity selection and/or analysis....

20060147939 - Compositions and methods for detecting human herpesviruses: The present invention relates to primer compositions and methods for identifying and distinguishing CMV, EBV-A/B, HSV-1/2, HHV-6 and VZV herpesviruses in a sample....

20060147989 - Compositions and methods for treatment of herpes: Prophylactic and treatment methods for treatment of herpes, as well as methods for reducing or preventing the transmission of herpes. The methods comprise the step of administering to a mammal, an amount of a composition having a first ingredient obtainable from ginger; a second ingredient obtainable from green tea; an...

20060147910 - Detection of mutations: The present invention relates to a polypeptide which is a flavin-containing monooxygenase 3 (FMO3), wherein said FMO3 is a polypeptide comprising at least a sequence having at least 85% identity with the polypeptide sequence SEQ ID NO: 15, in particular to a polypeptide which is a functionally altered mutant of...

20060147963 - Detection of polynucleotides on nucleic acid arrays using azido-modified triphosphate nucleotide analogs: Methods are provided for detecting hybridization of a polynucleotide to a nucleic acid array by chemically modifying the polynucleotide to contain a detectable label. According to one aspect of the present invention, a method is provided for detecting the presence of a mRNA in a nucleic acid sample, the method...

20060147977 - Device and methods for tracking conditions in an assay: The invention provides a device comprising a substrate having a plurality of different molecular probes attached to a surface thereof and an integrated indicator that exhibits a response when exposed to a condition to which the substrate may be exposed. Each different molecular probe is selected to interact with a...

20060147915 - Disease risk estimating method fusing sequence polymorphisms in a specific region of chromosome 19: The present invention provides methods and compositions for identifying human subjects with an increased risk of having or developing disease. In particular, this invention relates to the identification and characterization of polymorphisms in the human chromosome 19q, the region r located approximately 19q13.2-3 correlated with increased risk of developing disease,...

20060147912 - Dna amplification apparatus and method: The invention relates to a method for the amplification of DNA and apparatus for such amplification. The method comprises steps typically found in amplification methods but utilises an optical procedure to detect denaturation of DNA or attainment of the desired denaturation temperature. The apparatus (1) comprises a temperature controllable chamber...

20060147991 - Dna chip and reactive solid carrier: A reactive solid carrier favorably employable for manufacturing DNA chip is composed of a solid carrier and a plurality of vinylsulfonyl groups or their reactive precursors each of which is fixed onto a surface of the solid carrier by covalent bonding via a linking group, and a method for producing...

20060147948 - Fluorescent labeling of specific protein targets in vitro and in vivo: New methods are provided for the post-genomic era that will permit the analysis of the dynamic expression and localization of gene products in living cells. Herein we propose the development of such a method from a bioorganic approach involving organic synthesis and protein engineering. Specifically, novel compounds bearing two maleimide...

20060147986 - Gene and protein libraries and methods relating thereto: A set of libraries of proteins, which proteins are capable of specific binding interactions by virtue of amino acid residues at two or more determined positions including a first determined position and one or more other determined positions, which set of libraries consists of: a) 6 to 20 libraries in...

20060147956 - Genetic alterations associated with prostate cancer: The present invention provides new probes for the detection of prostate cancer cells. The probes bind selectively with target polynucleotide sequences selected from the group consisting of 2q, 4q, 5q, 6q, 10p, 15q, 1q, 2p, 3q, 3p, 4q, 6p, 7p, 7q, 9q, 11p, 16p, and 17q....

20060147962 - Genotype test: The present invention provides a method for assessing a nutritional requirement, disease susceptibility or behavioral characteristic of a dog, the method comprising: determining the nucleotide present at one or more SNP positions in the dog genome; identifying therefrom the genetic breed inheritance of the dog; thereby determining a nutritional requirement,...

20060147934 - High speed assay for glycosyl transferases: The polyprenyl phosphate: N-acetylhexosamine-1-phosphate transferase family are glycosyl transferase enzymes of interest in the development of antibacterial treatments. The bacterial proteins WecA and MraY are exemplary transferases, each of which catalyzes the transfer of a specific hexosamine 1-P from a soluble UDP-hexosamine substrate to a polyprenyl phosphate carrier at the...

20060147927 - High-sensitivity assays for pathogen detection using metal enhanced fluorescence: The present invention relates to an assay including a surface having silver colloids or islands attached thereto. Attached to the surface and/or silver colloids/islands are polynucleotides which are complimentary to a target polynucleotide sequence. The assay is performed by adding the target polynucleotide sequence to the assay surface and allowed...

20060147960 - Hybridization chamber agitation device using pump and valves: Provided is an agitation device used to agitate a solution in a hybridization chamber, the agitation device including: the hybridization chamber; first and second air channels connected to ends of the hybridization chamber; a first valve disposed in the first air channel; a second valve disposed in the second air...

20060147917 - Information processing system using base sequence relevant information: A highly safe system for processing information, which can provide useful semantic information for an individual and/or information associated with the semantic information via effective utilization of differences in nucleotide sequence information among individuals is constructed, This method comprises steps of: (a) receiving requested information for an object and/or service;...

20060147933 - Interspecies embryonic stem cell: Present invention demonstrates the derivation of ES cells from hybrid blastocysts, obtained by the mating of two different Mus species. The invention also relates to the use of these interspecies hybrid ES cells for the generation of genetically modified mammalian animals and for the identification of quantitative trait loci (QTL)...

20060147937 - Isolation of living cells and preparation of cell lines based on detection and quantification of preselected cellular ribonucleic acid sequences: The invention is directed to reliable and efficient detection of mRNAs as well as other RNAs in living cells and its use to identify and, if desired, separate cells based on their desired characteristics. Such methods greatly simplify and reduce the time necessary to carry out previously-known procedures, and offers...

20060147965 - Label free analysis of nucleic acids: A method is provided for label free analysis of nucleic acid materials. In accordance with the present invention, a fluorescent material is provided having a certain fluorescence emission without nucleic acids attached thereto. The fluorescent material of the present invention, has a greater emission upon the binding of a single...

20060147926 - Method and apparatus for performing multiple simultaneous manipulations of biomolecules in a two-dimensional array: This invention relates to methods and apparati for performing multiple simultaneous manipulations of biomolecules in a two-dimensional array, such as a gel, membrane, tissue biopsy, etc. Such manipulations particularly include assays and nucleic acid amplification protocols....

20060147975 - Method for assaying protein-protein interaction: The invention relates to a method for determining if a test compound, or a mix of compounds, modulates the interaction between two proteins of interest. The determination is made possible via the use of two recombinant molecules, one of which contains the first protein a cleavage site for a proteolytic...

20060147911 - Method for characterizing primary tumors: The invention relates to a method for the detection and characterisation of primary tumours and separate areas of primary tumours, respectively. Clusters of tumour cells, extracted from sample material, are isolated and concentrated, followed by an analysis of genetic changes in these isolated cell clusters....

20060147984 - Method for the detection of bacillus cereus: The present invention provides novel oligonucleotide primers for phosphotidyl inositol in B. cereus the primer comprising PI-1 (F) 5′ AGTATGGGGAATGAG 3′ and PI-2 (R) 5′ ACAATTTTCCCACGA 3′ and to a method for the detection of B. cereus in foods in a mixed microflora....

20060147929 - Method for the quantitative assessment of global and specific dna repair capacities of at least one biological medium, and the applications thereof: The invention relates to a method for the quantitative assessment of the global and specific DNA repair capacities of a biological medium, consisting in assessing the excision/resynthesis capacities of said medium, and to the applications thereof. The inventive method comprises the following steps: (a) a range of plasmids, each comprising...

20060147921 - Method of amplifying nucleic acid and apparatus therefor: In nucleic acid amplification, the amount of a labeled or modified oligomer used is reduced to lower cost, and detection is enabled within the measurement range of a detection unit without requiring operations such as dilution. A nucleic acid measurement method of the invention comprises the steps of extracting a...

20060147913 - Method of analyzing substance on substrate by mass spectrometry: Provided is a method making it possible to measure the molecular weight useful for the specification of a substance bonded on a substrate by MALDI-TOF MS. When the substance is to be bonded on the substrate, MALDI-TOF MS analysis can be utilized by providing a partial structure to be disconnected...

20060147976 - Method of detecting and quantitating microorganism having specific function and its gene from natural environment using novel 16srrna gene data or probes: A method of detecting and quantitating a petroleum-digesting microorganism and its gene from natural environment. In one embodiment, the microorganism is a bacterium belonging to the genus Cycloclasticus. The invention also relates to a method for detecting and quantitating a petroleum-degrading bacterium belonging to the genus Cycloclasticus comprising using an...

20060147925 - Method of detection: The present invention relates to a method of detecting a population of cells or microorganisms in a subject and, more particularly, to a method for qualitatively and/or quantitatively detecting a clonal population of cells or microorganisms in a subject. The method of the present invention is useful in a range...

20060147920 - Method of judging inflammatory disease: The object of the present invention is to determine inflammatory diseases such as myocardial infarction by identifying single nucleotide polymorphisms (SNPs) associated with myocardial infarction and utilizing these SNPs. The present invention provides a method for determining an inflammatory disease, which comprises detecting at least one gene polymorphism existing in...

20060147914 - Method of screening for agents that modulate immunophilin/peptidylproline cis-trans isomerase (ppiase)-homer interaction: The invention features a method of identifying, evaluating and screening for compounds or agents for the treatment of disorders involving the Homer signaling pathway in the modulation of immunosupression and neuroprotection. The method inlcudes evaluating the ability of agents to modulate Homer protein activity, Homer protein/immunophilin-peptidylproline cis-trans isomerase interaction, and/or...

20060147959 - Method to determine responsiveness of cancer to epidermal growth factor receptor targeting treatments: The present invention is directed to a method for determining the responsiveness of cancer to an epidermal growth factor receptor (EGFR) treatment. In a preferred embodiment, the presence of at least one variance in the kinase domain of the erbB1 gene confers sensitivity to the tyrosine kinase inhibitor gefitinib. Thus,...

20060147941 - Methods and apparatus for sers assay of biological analytes: SERS technology is used for high throughput screening of biological analytes and samples. For polynucleotide sequencing, sets of oligonucleotide probes are labeled with composite organic-inorganic nanoparticles (COIN) that produce distinguishable SERS signals when excited by a laser. Detection of a hybridization complex containing members of two such COIN-labeled probe sets...

20060147985 - Methods and compositions for monitoring polymer array synthesis: VLSIPS™ manufacturing processes are of increasing commercial importance. The present invention provides methods and compositions for monitoring the efficiency and quality of polymer synthesis in VLSIPS™ arrays. Methods for monitoring polymer synthesis in an array on a substrate are provided. Mono-isomeric labels for the labeling of synthetic polymer arrays are...

20060147978 - Methods and compositions for screening for altered cellular phenotypes: The invention relates to methods and compositions useful for screening for altered cellular phenotypes using an inducible expression system to enrich for and detect the altered phenotypes and, more particularly, relates to screening libraries of candidate bioactive agents, for example, nucleic acids and peptides, in cells using an regulatable expression...

20060147935 - Methods and means for nucleic acid sequencing: Nucleic acid sequencing-by-synthesis. Primed synthesis of a second strand complementary to a template strand in repeated sets of steps, each step comprising providing one or more of the possible nucleotide complementarity classes for incorporation into the synthesized strand, and each set of steps comprising providing all four possible nucleotide complementarity...

20060147981 - Methods for array preparation using substrate rotation: Methods are provided for the preparation of polymer arrays on wafers wherein the wafers are rotated between synthesis steps to provide more uniform results across the entire wafer....

20060147907 - Methods for detection of promoter polymorphism in a ugt gene promoter: The present invention is directed to methods for detecting the presence of genetic polymorphisms that correlate with altered gene expression. More specifically, the present invention is directed to methods for detecting the genetic polymorphisms located in the UGT1A1 promoter. The invention also provides methods for optimizing drug dosages based upon...

20060147979 - Methods for forming recombined nucleic acids: Described herein are methods for generating recombined nucleic acids. In one method, fragments of a sequence are provided wherein the fragments have non-extendable 3′ ends. A primer is provided and the primer and fragments are reacted under conditions to extend the primer to form a recombined nucleic acid molecule. In...

20060147957 - Methods for high throughput sample preparation for microarray analysis: Automated methods for sample preparation for amplification of nucleic acid samples to prepare target for hybridization to microarrays are disclosed. Automated methods for hybridizing target to microarrays, washing and staining microarrays are also disclosed. Improved conditions for hybridization and for storage and scanning of arrays with hybridized target are also...

20060147980 - Methods for isolating and characterizing endogenous mrna-protein (mrnp) complexes: Cellular mRNA-protein (mRNP) complexes are partitioned in vivo by contacting a biological sample with at least one ligand that specifically binds at least one component of a mRNP complex. Suitable biological samples comprise at least one mRNA-protein (mRNP) complex and include cell cultures, cell extracts, and whole tissue, including tumor...

20060147961 - Methods for ligation independent cloning of dna: The present invention provides methods for assembling DNA molecules in a predetermined order to produce a DNA construct useful in Agrobacterium mediated transformation in plants. The method employs ligation independent cloning of separate DNA elements where one of the DNA elements contains T-DNA borders from Agrobacterium tumefaciens. The invention further...

20060147953 - Methods of diagnosing cardiovascular disease: Described herein are methods for diagnosing increased risk of cardiovascular disease in a subject, based on the presence or absence of polymorphisms in the D36 gene....

20060147932 - Methods of screening for modulators of nerve growth factor: The invention relates, in general, to a method of screening for agents that modulate NGF activity. More specifically this invention provides a method of assessing the ability of a candidate agent to modulate NGF activity comprising, measuring the level of expression of one or more or two or more NGF...

20060147909 - Microstructures and use thereof for the directed evolution of biomolecules: The invention relates to microstructures and the use thereof for the directed evolution of biomolecules....

20060147952 - Modulation of exon recognition in pre-mrna by interfering with the secondary rna structure: The invention provides a method for generating an oligonucleotide with which an exon may be skipped in a pre-mRNA and thus excluded from a produced mRNA thereof. Further provided are methods for altering the secondary structure of an mRNA to interfere with splicing processes and uses of the oligonucleotides and...

20060147964 - Mycobacterial rpob sequences: This invention provides polynucleotide probes, sequences and methods for speciating and phenotyping organisms, for example, using probes based on the Mycobacterium tuberculosis rpoB gene. The groups or species to which an organism belongs may be determined by comparing hybridization patterns of target nucleic acid from the organism to hybridization patterns...

20060147988 - Netrin receptors: The invention provides methods and compositions relating to vertebrate UNC-5 proteins which function as receptor proteins for netrins, a family of cell guidance proteins. The proteins may be produced recombinantly from transformed host cells from the disclosed vertebrate UNC-5 encoding nucleic acid or purified from human cells. The invention provides...

20060147946 - Novel calcium channel variants and methods of use thereof: Novel alpha 1 subunit splice variants, including nucleic and amino acid sequences. Methods of use thereof are also described....

20060147922 - Novel diagnostic and therapeutic methods and reagents therefor: This invention provides novel methods of detecting or treating aberrant cell cycle regulation associated with expression of a nuclear protein encoded by a gene that is linked to map position 8q22.3 of the human genome, and to novel reagents that are useful therefor. More particularly, the invention provides novel nucleic...

20060147974 - Novel drosophila tumor necrosis factor class molecule (\"dmtnf\") and variants thereof: The present invention provides novel polynucleotides encoding Drosophila DmTNF polypeptides, fragments and homologs thereof. The present invention also is directed to novel polynucleotides encoding two Drosophila DmTNF variants, DmTNFv1 and DmTNFv2 polypeptides, fragments and homologs thereof. Also provided are vectors, host cells, antibodies, and recombinant and synthetic methods for producing...

20060147954 - Novel probe and its use in bioaffinity assays: The invention concerns a labeled probe for use in bioaffinity assays based on time-resolved fluorescence resonance energy transfer, wherein said probe comprises an energy donor as well as an energy acceptor, and the energy transfer signal of the acceptor is to be measured. The invention concerns also a homogeneous time-resolved...

20060147928 - Novel real-time rt-pcr for the sensitive detection of multiple mage gene transcripts: The present invention relates to a highly sensitive real-time RT-PCR method for specifically detecting the expression of more than one MAGE gene. The present invention further relates to a diagnostic composition for carrying out such a real-time RT-PCR as well as to oligonucleotides suitable for the cDNA synthesis reaction prior...

20060147945 - Novel secreted proteins and their uses: The present invention provides nucleic acid sequences encoding novel human proteins. These novel nucleic acids are useful for constructing the claimed DNA vectors and host cells of the invention and for preparing the claimed nucleic acids, recombinant proteins and antibodies that are useful in the claimed methods and medical uses....

20060147951 - Novel serpentine transmembrane antigens expressed in human cancers and uses thereof: Described is a novel family of cell surface serpentine transmembrane antigens. Two of the proteins in this family are exclusively or predominantly expressed in the prostate, as well as in prostate cancer, and thus members of this family have been termed “STRAP” Serpentine TRansmembrane Antigens of the Prostate). Four particular...

20060147983 - Nucleic acid detecting sensor, nucleic acid detecting chip, and nucleic acid detecting circuit: Nucleic acid detecting sensor includes field-effect transistor, detector which detects target nucleic acid molecules having sequences from sample based on degree of a variation in threshold voltage of field-effect transistor, and at least one nucleic acid probe molecule which is hybridized with corresponding one of target nucleic acid molecules, and...

20060147923 - Nucleic acid sequences encoding novel point mutations on mglur2 and mglur3: The present invention is directed to mutant forms of mGluR2 and mGluR3 that are shown to affect the binding of modulators of said receptors. The invention discloses polypeptides comprising the mutations, and nucleic acid sequences that encodes said polypeptides, and methods of using said polypeptides and nucleic acids sequences to...

20060147971 - Parallel preparation of high fidelity probes in an array format: The present invention provides massively parallel oligonucleotide synthesis and purification for applications that utilize large collections of defined high-fidelity oligonucleotides (e.g., from about 101 to about 105 different sequences, generally between 25-160 bases in length)....

20060147967 - Phi 15 dna polymerase: A purified recombinant DNA polymerase having high processivity and strand-displacement activity. An isolated nucleic acid that encodes the bacteriophage DNA polymerase. A kit and method for amplifying DNA is also disclosed....

20060147949 - Pna chip using plastic substrate coated with epoxy group-containing polymer, method of manufacturing the pna chip, and method of detecting single nucleotide polymorphism using the pna chip: Provided is a PNA (Peptide Nucleic Acid) chip in which a probe PNA containing a desired DNA sequence is immobilized on a plastic substrate coated with an epoxy group-containing polymer. Therefore, single-stranded PNAs can be immobilized on a transparent plastic substrate by means of an epoxy group-containing polymer layer in...

20060147973 - Polynucleotide encoding a novel human serpin secreted from lymphoid cells, lsi-01: The present invention provides novel polynucleotides encoding LSI-01 polypeptides, fragments and homologues thereof. Also provided are vectors, host cells, antibodies, and recombinant and synthetic methods for producing said polypeptides. The invention further relates to diagnostic and therapeutic methods for applying these novel LSI-01 polypeptides to the diagnosis, treatment, and/or prevention...

20060147924 - Population of nucleic acids including a subpopulation of lna oligomers: The present invention relates to a population of nucleic acids, said population comprising a first population of nucleic acids of the same length, said length being in the range of 5-15 nucleotides or units, said first population representing at least 1% of the possible different nucleic acid sequences for nucleic...

20060147950 - Precursor conversion screening method: The invention provides methods of identifying a host cell that encodes a metabolic pathway that converts a precursor molecule into a growth inhibitory compound, by: (a) culturing a population of host cells under conditions that allow expression of the metabolic pathway; (b) contacting the host cells, or an extract thereof,...

20060147966 - Preparation and labeling of polynucleotides for hybridization to a nucleic acid array: In accordance with the present invention, method are presented for labeling a cDNA strand with a photochemical cleavable reagent which upon exposure to electromagnetic radiation of particular reagent to create abasic DNA sites. According to one aspect of the present invention, DNA at the abasic sites, also known a chemical...

20060147969 - Primer array synthesis and validation: Methods are presented for generating large sets for polymers. The methods employ high density oligonucleotide array....

20060147916 - Probe carrier and method of producing same: A method of producing a probe carrier in which a probe is immobilized to a substrate is disclosed, which comprises providing the substrate and contacting a basic group introduced to the substrate with the probe having an acidic group to thereby immobilize the probe to the substrate. This method enables...

20060147990 - Process for producing template dna and process for producing protein in cell-free protein synthesis system with the use of the same: Provided is a method of producing a template DNA used for protein synthesis comprising a step of amplifying a linear double-stranded DNA by polymerase chain reaction (PCR), by using a reaction solution comprising a first double-stranded DNA fragment comprising a sequence coding for a protein or a portion thereof, a...

20060147944 - Reagents and methods for storage and processing of biological samples for dna analysis: The present invention describes reagents and methods for storing and/or processing of biological samples for direct use in PCR and other DNA applications. After storage, the preserved DNA in the samples may then be processed and analyzed by known methods, e.g., PCR. the present invention provides in one aspect, a...

20060147955 - Single step detection assay: The present invention provides methods and routines for developing and optimizing nucleic acid detection assays for use in basic research, clinical research, and for the development of clinical detection assays. In particular, the present invention provides methods for designing oligonucleotide primers to be used in multiplex amplification reactions. The present...

20060147918 - Stabilisation of nucleic acids: The present invention concerns a method for the stabilisation of nucleic acid from a biological sample, which comprises: (a) collecting of biological sample; (b) treating the sample so that a proportion of the 2′, 3′ or 5′-OH positions of the nucleic acid are modified with a protecting group; and (c)...

20060147942 - Stabilizing a nucleic acid for nucleic acid sequencing: The invention provides methods for sequencing a nucleic acid comprising stabilizing a primer/target nucleic acid duplex on a substrate. Methods of the invention generally contemplate the use of a dual-anchored primer/target nucleic acid duplex, or a stabilizing molecule in a single molecule sequencing reaction....

20060147943 - Substrates having pendant epoxide groups for binding biomolecules and methods of making and using thereof: Described herein are substrates having one or more pendant epoxide groups capable of being attached to one or more different biomolecules and methods of making and using thereof....

20060147958 - System for detecting polynucleotides: The present invention relates to methods for detecting the presence or amount of a target polynucleotide. A polynucleotide, target nucleic acid analog, and dye are combined to form a mixture. The optical property of the dye is observed after the mixture is exposed to a stimulating means. Optionally, after the...

20060147936 - Use of a gene mutation in the human gnas gene for predicting risks of diseases, courses of the disease and for predicting the response to disease therapies: The invention concerns the use of a genomic gene modification in the gene for the Gas subunit of the human G protein, coded by the gene GNAS (or GNAS1), for the prediction of disease risks, disease clinical courses and responses to pharmacological and non-pharmacological measures and for the prediction of...

20060148005 - Biologically active synthetic thyrotropin and cloned gene for producing same: Substantially pure recombinant TSH has been prepared from a clone comprising complete nucleotide sequence for the expression of the TSH. Diagnostic and therapeutic applications of the synthetic TSH are described....

20060148001 - Compositions and methods for treating female fertility: The PTX3 gene or equivalent PTX3 activity is required for female fertility. Manipulation of PTX3 activity will regulate female fertility. The effects of female sterility may be ameliorated, reproductive ability may be increased or decreased as desired, female fertility may be enhanced, or combinations thereof. The need for therapies that...

20060147996 - Device for holding and detecting substance libraries: The invention relates to a holding device for substance libraries which can be used, more particularly, for the detection of target molecules with the aid of substance libraries. Said device comprises: (i) a holder for a substance library carrier; (ii) a substance library carrier, which can be fixed to the...

20060147993 - Membrane arrays and methods of manufacture: The invention relates to G protein-coupled receptor (GPCR) microarrays on porous substrates for structural or functional analyses of GPCRs, and methods of preparing porous substrate surfaces for receiving membranes that comprise GPCRs. In one embodiment, a GPCR microarray of the invention comprises a membrane adhered to an upper surface of...

20060147999 - Method and apparatus for homology-based complex detection in a protein-protein interaction network: Provided are a method and a apparatus for detecting a protein complex using a similarity between different proteins in a protein-protein interaction network. The method includes: (a) producing a virtual complex of a specific organism by mapping proteins contained in a complex of a different organism into proteins of the...

20060147992 - Method for detection of bioactive peptides: A method for the detection of bioactive peptides derived from a precursor protein or protein-containing biological extract, comprising the steps of: (i) providing a library of peptides derived from said precursor protein or protein-containing biological extract; (ii) optionally screening said library to confirm that it includes peptides exhibiting one or...

20060147998 - Method for diagnosing and monitoring critically ill patients: Provided is an improved method of diagnosing and monitoring hemostatic dysfunction, sepsis-related morbidity or severe infection by improving detection of an in vitro complex formed by lipoprotein and C-reactive protein with the utilization of an effective amount of a surface active agent in the reagent....

20060147995 - Method for producing antibody fragments: An expression library comprising a repertoire of nucleic acid sequences each encoding at least part of a variable domain of a heavy chain derived from an immunoglobulin naturally devoid of light chains and its use in producing antibodies, particularly fragments thereof, is disclosed. The invention provides a method for preparing...

20060148002 - Methods and compositions for the treatment and diagnosis of body weight disorders: The invention relates to methods and compositions for the treatment and diagnosis of body weight disorders, including, but not limited to, obesity, overweight, anorexia, cachexia, insulin resistance, and diabetes. The invention further provides methods for identifying a compound capable of treating a body weight disorder. In addition, the invention provides...

20060148000 - Methods for identifying modulators of n-type ion channel inactivation: Methods and compositions for identifying compounds which disrupt the functional interaction of an intracellular receptor region of an a-subunit of a voltage gated ion channel and an amino-terminal inactivation region of an ion channel protein are disclosed. Compounds that disrupt the functional or binding interaction of these two regions have...

20060147997 - Penetrabodies: receptor-mediated targeted delivery of functionally-active human antibody fragments into cytosol for the treatment of chronic infections and diseases: The present invention relates to methods of making and using chimeric antibody molecules comprised of at least two domains, namely RAF, and IAF, linked by a flexible peptide linker....

20060148003 - Peptide sequence tags and method of using same: Peptide sequence tags are identified and used to produce a class of global antibodies, which recognize key protein subunits of the ATP Synthase complex with uniform specificity, regardless of the species of origin. The tags are used to create antibodies to the major AtpB subunit or the AtpC subunit of...

20060148004 - Peptide sequence tags and method of using same: Peptide sequence tags are identified and used to produce a class of global antibodies, which recognize all members of key protein subunit families for the PSI complex with uniform specificity, regardless of the species of origin. The tags are used to create antibodies to the key PsaC or PsaA proteins...

20060148006 - Assay solution compositions and methods for gpcr arrays: Buffered assay solutions for performing 1) binding or 2) functional assays on GPCR arrays, along with methods for their use are described. The buffered assay solution has an underlying composition having: a buffer reagent with a pH in the range of about 6.5 to about 7.9; an inorganic salt of...

20060148007 - Method for using division arrested cells in screening assays: Division arrested cells are used in screening assays to determine the effect of a substance of interest on the cells. The division arrested cells can be used in drug screening assays, signal transduction assays, and are especially useful in large scale, high throughput assays....

20060148008 - Method of characterizing dendritic cells: A method of characterizing cells in a subclass of dendritic cells or precursors thereof in a biological sample. The method comprising placing the sample in contact with immunointeractive molecules directed to at least one dendritic cell immunogen (e.g. HLA-DR, CE123, CD11c, CD1b/c, BDCA2, BDCA3, BDCA4, Cb16, CD83, CD45, CD40, CMRF-44...

20060148009 - Prediction and assessment of immunogenicity: A system and method to predict and assess immunogenicity, especially prior to on-set of immunogenic conditions. Also disclosed are methods to identify relevant peptides associated with the formation of antibodies in patients treated with a given protein therapeutic. In various aspects, the present application is directed to methods of determining...

20060148010 - Isolation and purification of p. falciparum merozoite protien-142 vaccine: In this application is the expression and purification of a recombinant Plasmodium falciparum (3D7) MSP-142. The method of the present invention produces a highly purified protein which retains folding and disulfide bridging of the native molecule. The recombinant MSP-142 is useful as a diagnostic reagent, for use in antibody production,...

20060148012 - Assay for identifying antibody producing cells: The present invention provides a homogeneous assay for identifying an antibody producing cell producing an antibody which binds to a selected antigen comprising: a) providing a population of antibody producing cells; b) incubating said population of antibody producing cells with a selected antigen and a labeled anti-antibody antibody, wherein said...

20060148011 - Early prostate cancer antigens (epca), polynucleotide sequences encoding them, and their use: A novel prostate cancer marker is described that is found in cancerous and normal prostate cells of individuals that have prostate cancer but is not found in the prostate of individuals without prostate cancer. The marker also is present in normal tissue adjacent to tumor tissue in individuals having prostate...

20060148014 - Tubulin isotype screening in cancer therapy using hemiasterlin analogs: Chemotherapeutic agents that interfere with microtubule assembly or disassembly in the cell are potent inhibitors of cell replication. Examples of such agents include hemiasterlin analogs. It has been shown that the susceptibility of certain cancers to analogs of hemiasterlin correlates with the expression of particular tubulin isotypes or other microtubule-associated...

20060148013 - Tumor marker and its use: The invention provides a new kind of tumor marker RL9/RL10 protein, the polynucleotide encoding the polypeptide, and the method of producing RL9/RL10 protein by recombinant technology. The invention also discloses the use of RL9/RL10 protein and the polynucleotides encoding RL9/RL10 protein, e.g., in diagnosing and treating tumor, as well as...

20060148015 - Lectin pathway deficiency assay: The invention refers to an in vitro method of functionally determining at physiological conditions deficiencies in the lectin pathway of the complement system, the method comprising the steps of (a) providing a sample of mammalian blood, serum, plasma or another body fluid; (b) preventing in the sample the activation of...

20060148017 - Compositions and methods for monitoring enzyme activity: The invention provides a method to monitor the activity of an enzyme comprising the step of monitoring the addition to at least one polypeptide of a phosphate moiety. The polypeptide is an isolated polypeptide, or a fragment thereof, comprising a non-natural site sufficient for the addition of a phosphate (PO4)....

20060148018 - Insulin-like growth factor binding protein-4 protease: Methods for screening for altered focal proliferation states in non-pregnant patients, which include detecting levels of pregnancy-associated plasma protein-A (PAPP-A), are described. Methods for identifying agents that alter the protease activity of PAPP-A, and pharmaceutical compositions and medical devices that include such agents also are described....

20060148016 - Nanoparticle for bioaffinity assays: Thus this invention relates to a nanoparticle, useful for bioaffinity assays. The nanoparticle has a self-assembling shell built up of several protein and/or peptide subunits, which protein and/or peptide subunits can be of one or several different types, assembled in an organized manner to form the shell having an inner...

20060148019 - Luminescence-based recipe and device using the same: Luminescence-based recipes for the measurement of creatinine, urea, uric acid, or glucose, and device using the same are provided....

20060148020 - Inhibitors of gst a3-3 and gst a1-1 for the treatment of cancer: The present invention relates to a novel drug target, glutathione transferase (GST), preferably GST A3-3, as target for treatment of cancer and other diseases responsive to inhibition of steroid hormone production. The present invention also relates to a method for screening of compounds or drug candidates that modulate, preferably inhibit,...

20060148023 - Affinity labeling of serine proteases for simultaneous detection of multiple serine protease activity levels: The invention provides assay methods and reagents useful for evaluating the level of enzyme activities within living cells. Enzyme activity levels within living cells, such as Serine proteases, can be key determinates in assessing; 1) the presence of tumor (cancer) cells, 2) the predictive efficacy of a chemotherapeutic treatment regimen...

20060148022 - Assay for tissue factor and factor viia: The invention provides assays for detecting and quantitating tissue factor and factor VIIa in simple and complex biological systems. The assays are performed by detecting and/or measuring the tissue factor cofactor activity and factor VIIa enzymatic activity using aminonapthalene-based fluorogenic substrates....

20060148021 - Extraction of a proteinaceous macromolecule from a biological sample: The invention provides a method of treating a biological sample to extract a macromolecule under acidic conditions. In one aspect the present invention provides a method for enhancing the solubilisation of at least one proteinaceous macromolecule in a biological sample without inducing substantial acid hydrolysis of said proteinaceous macromolecule, said...

20060148026 - Engineering redox proteins: The protein of this invention comprises a 4-α-helix bundle motif formed from the α-helices of rop (repressor of primer) and a redox centre. In this invention the redox centre is preferably haem and the iron is preferably coordinated to the α-helices of the rop structure via histidine residues. Such a...

20060148024 - Homogenous assay for enzymatic activity: An assay is disclosed for measuring activity of enzymes, such as kinases, phosphatases, and proteases. Measurements of enzymatic activity are accomplished in a homogenous assay format utilizing a fluorescence quenching technique employing paramagnetic metal ions....

20060148025 - Method of analyzing coenzyme q-10 and two-electron reduction product thereof and analysis system: The present invention relates to an analysis method and analysis system that can accurately analyze the contents of coenzyme Q-10 and a 2-electron reduced form thereof in a specimen, in which human blood plasma as the specimen is mixed with isopropyl alcohol as a pretreatment and the coenzyme Q-10 and...

20060148027 - Sprouty and spred protein biosensors: Sprouty/SPRED protein cysteine-rich domain (SCRD) modules comprising an iron:sulfer complex are used to sense electro/chemical signals....

20060148028 - Method for detecting microbe or cell: A method for detecting microorganisms or cells wherein microorganisms or cells contained in a sample are captured on the surface of the adhesive layer of a collection sheet having a substrate layer containing a focusing marker, an adhesive layer having a predetermined thickness and deposited on this substrate layer, the...

20060148033 - Cold-induced expression vector: A vector having a region encoding a cold shock protein gene mRNA-origin 5′-nontranslated region, characterized in that the 5′-nontranslated region has a mutation having been transferred therein so as to change the distance of the stem structure formed by the region....

20060148036 - Gabaalpha receptor epsilon subunits: The present invention relates to a novel GABAA receptor ε subunit (GABRE) and an alternative transcript thereof (ET2). More specifically, isolated nucleic acid molecules are provided encoding human GABRE and ET2 receptor subunits. ET2 and GABRE polypeptides are also provided, as are vectors, host cells and recombinant methods for producing...

20060148031 - Hemipteran myosin light chain kinase: Novel nucleic acid sequences encoding hemipteran myosin light chain kinases are disclosed. Isolated hemipteran myosin light chain kinases are also disclosed....

20060148029 - Lactoferricin gene and transformant expressing lactoferricin: Disclosed is a gene encoding a mutated lactoferricin peptide and an E. coli cell transformed with the same, wherein the mutated lactoferricin peptide has substitutions at the 7th and 10th amino acid residues of the wildtype bovine lactoferricin. The gene of the present invention can be expressed in E. coli...

20060148034 - Method for preventing and/or treating viral infection in aquatic animals: The present invention provides a preferred method for preventing and/or treating a virus infection in aquatic animals, including the steps of: (i) providing a composition including trichoanguin protein; (ii) adding the composition to an aqueous solution including aquatic animals; and (iii) exposing the aquatic animals to the composition; wherein the...

20060148035 - Methods for producing modified glycoproteins: Cell lines having genetically modified glycosylation pathways that allow them to carry out a sequence of enzymatic reactions, which mimic the processing of glycoproteins in humans, have been developed. Recombinant proteins expressed in these engineered hosts yield glycoproteins more similar, if not substantially identical, to their human counterparts. The lower...

20060148032 - Novel phosphoprotein: The present invention relates generally to cancer therapy and cancer diagnostics and to agents useful therefor. More particularly, the present invention provides a novel tumorigenesis-related phosphoprotein and a nucleic acid molecule encoding same. The present invention also provides diagnostic agents to detect the presence or absence of the tumorigenesis-related phosphoprotein...

20060148030 - Nuclear receptor err y 3: The present invention relates to novel nuclear receptor ERRγ3. Although ERRγ3 itself lacks a DNA binding domain, it comprises the function of enhancing the transcriptional activation function of arbitrary nuclear receptors, such as ERR, ER, or TR. Moreover, the present inventors found that, like ERRγ3, the known proteins ERRγ1 and...

20060148037 - Polynucleotides encoding potassium channels: The present invention provides polynucleotides encoding potassium channels as well as methods of using the potassium channels to treat and diagnose diabetes and a predisposition for diabetes....

20060148038 - Thrombin cleavable factor x analogues: The invention relates to factor X analogues containing the thrombin-cleavable sequence Pro-Arg-Ala in place of the-sequence Thr-Arg-Ile of the activation site of native factor X. These factor X analogues can be used to obtain procoagulant medicinal products....

20060148039 - Methylotroph producing mammalian type sugar chain: This invention is to provide a process for producing a glycoprotein comprising a mammalian type sugar chain, characterized in that the process comprises introducing an α-1,2-mannosidase gene into a methylotrophic yeast having a mutation of a sugar chain biosynthesizing enzyme gene, so that the α-1,2-mannosidase gene is expressed under the...

20060148040 - Process for preparing isomalto-oligosaccharides with elongated chain and low glycemic index: The invention relates to a process for preparing isomalto-oligosaccharides with elongated chain length. Isomalto-oligosaccharides are directly converted to isomalto-oligosaccharides with elongated chain length in the presence of glucansucrase. Said products can be applied in food, feed, beverages, cosmetics or pharmaceutical products and are particularly useful as slow or non-digestible oligosaccharides,...

20060148041 - Method for fermentative production of amino acids and amino acid derivatives of the phosphoglycerate family: A microorganism strain suitable for fermentative production of amino acids of the phosphoglycerate family or derivatives thereof and producible from a starting strain, is based upon a starting strain having an increased activity of a yfiK-gene product or of a gene product of a yfiK homologue. The microorganism strain is...

20060148044 - Enzymatic production of macrocyclic amide oligomers: Hydrolytic enzymes capable of catalyzing amidation reactions can be used to produce cyclic amide oligomers from diesters and diamines. The cyclic amide oligomers are useful for the production of higher molecular weight polyamides....

20060148043 - Novel optically active, heteroaromatic beta-hydroxy esters and processes for their preparation from beta-keto esters and processes for the preparation of these beta-keto esters: The present invention relates to new optically active heteroaromatic β-hydroxy esters useful in the synthesis of epothilone derivatives, to certain compounds used to produce these intermediates, as well as to processes for their production....

20060148042 - Process for preparing chiral aromatic alpha-hydroxy ketones using 2-hydroxy-3-oxoacid synthase: A biotransformation process for preparing chiral aromatic-hydroxy ketones in high yields is described, using 2-hydroxy-3-oxoacid synthase, such as AHAS or TSAS. Optionally substituted arylaldehydes and -oxoacids react in this process to provide pure enantiomers, useful as synthons in the production of various drugs, an example being (R)-phenylacetyl carbinol....

20060148045 - Composition containing lactic acid bacterium producing equol: The present invention provides an equol-producing lactic acid bacteria-containing composition comprising, as an essential component thereof, a lactic acid bacterial strain belonging to the genus Lactococcus having an ability to utilize at least one daidzein compound selected from the group consisting of daidzein glycosides, daidzein, and dihydrodaidzein to produce equol....

20060148046 - Enzymatic process for the preparation of an intermediate compound and use thereof for the synthesis of tamsulosin hydrochloride: e

20060148047 - Lipase-catalysed esterification of marine oil: Marine oil compositions which contain EPA and DHA as free acids or hexyl esters are esterified with ethanol in the presence of a lipase catalyst under essentially organic solvent-free conditions and separated by distillation....

20060148048 - Process for acyl-transfer enzyme reactions with acyl-coenzyme a: The present invention relates to a method for acyltransferase reaction in which an acyl group of acyl coenzyme A is transferred to an acyl group receptor characterized in that the reaction is carried out by production and/or reproduction of acyl coenzyme A from coenzyme A in a reaction system by...

20060148049 - Gene disrupted yeast: In the practice of the invention, a yeast strain in which ADE1 gene has been disrupted is constructed by homologous recombination with a chromosomal DNA by using an ADE1 gene fragment encoding phosphoribosylaminoimidazole succinocarboxamide synthase (EC6.3.2.6) of Candida maltosa. In the practice of the invention, then, this disrupted strain is...

20060148050 - Yeast strains for the production of lactic acid: Yeast strains transformed with at least one copy of a gene coding for lactic dehydrogenase (LDH) and further modified for the production of lactic acid with high yield and productivities, are described....

20060148051 - Method for the microbiological isomerisation of alpha-hydroxy carboxylic acids: The present invention relates to a method for the microbiological isomerization of alpha-hydroxycarboxylic acids using an alpha-hydroxycarboxylic acid racemase, the enzymes used for this method and microorganisms which express a suitable racemase activity, a screening method for microorganisms with alpha-hydroxycarboxylic acid racemase activity, the nucleic acid sequences encoding this enzyme,...

20060148052 - Production of polyketides in bacteria and yeast: Hybrid and novel polyketide synthases and polyketides are produced by use of a multiple vector system. The combinatorial possibilities offered by placing the various catalytic activities of PKS systems on separate vectors permits the construction of improved libraries of PKS and polyketides. In addition, polyketides can be produced in hosts...

20060148053 - Process for the biological production of 1,3-propanediol with high titer: The present invention provides an improved method for the biological production of 1,3-propanediol from a fermentable carbon source in a single microorganism. In one aspect of the present invention, an improved process for the conversion of glucose to 1,3-propanediol is achieved by the use of an E. coli transformed with...

20060148054 - Enzymes for starch processing: The present invention relates to polypeptides comprising a carbohydrate-binding module amino acid sequence and,an alpha-amylase amino acid sequence as well as to the application of such polypeptides....

20060148056 - Forms of soluble pyrroloquinoline quinone-dependent glucose dehydrogenase: The present invention relates to improved variants of soluble pyrroloquinoline quinone (PQQ)-dependent glucose dehydrogenases (s-GDH), to genes encoding mutated s-GDH, to mutant proteins of s-GDH with improved substrate specificity for glucose, and to different applications of these s-GDH variants, particularly for determining concentrations of sugar, especially of glucose in a...

20060148055 - Oxidised fragments of apolioprotein b and their uses: A molecule having the sequence of SEQ ID NO: 1 lysine 5 being conjugated with MDA is provided. The molecule inhibits uptake by the high affinity LDL receptor of LDL or a partially modified form thereof....

20060148057 - Thioredoxin mutants and uses thereof: The invention relates to an isolated mutant thioredoxin (Trx) molecules and related molecules, including, for example, a thioredoxin mutant resistant to the oxidizing effects of cytokines or reactive oxygen species. The invention further provides various uses, including treatment and diagnostic uses....

20060148058 - Organic compounds: The tides which comprise an amino acid sequence consisting of a mutated functional Abl kinase domain, said mutated functional kinase domain being resistant to inhibition of its tyrosine kinase activity by N-[4-methyl-3-(4-pyridin-3-yl-pyrimidin-2-ylamino)-phenyl]-4-(4-methyl-piperazin-1-ylmethyl)-benzamide or a salt thereof, to the use of such polypeptides to screen for compounds which inhibit they tyrosine...

20060148059 - Salt tolerant l-myo-inositol 1-phosphate synthase and the process of obtaining the same: A salt tolerant L-myo-inositol 1-phosphate synthase for Porteresia coarctata (PINO1), the nucleotide sequences and the deduced aminoacid sequence which is shown later....

20060148060 - Biomolecule transduction motif mph-1-btm and the use thereof: This invention relates to a novel Biomolecule Transduction Motif (BTM), Mph-1 peptide, which has the potential to transduce many biological response modifiers effectively into the cytoplasm, intracellular organelles or nucleus of prokaryotic or eukaryotic cells in vivo and in vitro, and the related technological methods using Mph-1 BTM. This Mph-1...

20060148061 - Gene sequence of l-rhamnose isomerase having new catalytic function and use thereof: In the rare sugar strategy of Izumoring (FIG. 1), it is intended to establish a reaction system of producing rare sugars of many types by acquiring an isomerase which acts on various rare aldoses and, therefore, is most efficient in producing various rare ketoses. A DNA encoding the following protein...

20060148062 - Systems and methods for ex-vivo organ care: The invention provides, in various embodiments, systems, devices and methods relating to ex-vivo organ care. In certain embodiments, the invention relates to maintaining an organ ex-vivo at near-physiologic conditions....

20060148063 - Method and apparatus for automated pre-treatment and processing of biological samples: A method and apparatus for continuous workflow processing of biological samples. In one embodiment, the appratus includes a probe for dispensing one or more reagents from one or more reagent containers onto one or more biological sample carriers. The method and apparatus includes processing each biological sample according to a...

20060148064 - Apparatus and method for immunotherapy of a cancer through controlled cell lysis: The treatment device includes an extraction mechanism, configured to extract a tissue sample from a patient or a tumor, coupled to a lysis mechanism. The lysis mechanism is configured to induce lysis of the tissue sample into a lysed tissue sample. The treatment device also includes an administration mechanism that...

20060148067 - Bio-bonding detection apparatus using ultrasonic waves and method thereof: An apparatus for detecting bio-bonding using ultrasonic waves and a method thereof are disclosed. The apparatus includes a generator for generating ultrasonic wave, a transformer, disposed in proximity to the generator, the transformer configured for vibration thereof by the generated ultrasonic wave. A probe biomolecule is immobilized on the transformer,...

20060148065 - Collection of micro scale devices: A collection of one or more microfluidic devices which together carry a plurality of microchannel structures each of which comprises a reaction microcavity (104a-h) in which there is a solid phase with an immobilized affinity ligand L, characterized in that (i) the plurality is divided into sets of microchannel structures,...

20060148066 - Electrospun nanofibrous membrane assembly for use in capturing chemical and/or biological analytes: A membrane assembly adapted for use in capturing an analyte of interest, the membrane assembly comprising in one embodiment (a) an electrospun nanofibrous membrane, the electrospun nanofibrous membrane comprising a random mat of electrospun nanofibers, at least some of the electrospun nanofibers including one or more types of functional groups;...

20060148068 - Biological sensor: In the same body 2, this detector integrates means to extract a sample from the environment, be it solid, liquid or gaseous; means 4 for the biological culture or magnification of said sample; detection means 5 inducing a reaction, for example on an impregnated strip 12, said reaction being either...

20060148069 - Biochemical container: In order to provide a biochemical vessel suitable for use in effective cell culture, precision analysis, etc., in a biochemical vessel comprising a plate-like body defining a plurality of through holes along a thickness thereof, and a substrate bonded to one face of the plate-like body via an adhesive, the...

20060148070 - Consumable for laser capture microdissection: Systems and methods for acquiring laser capture microdissection samples are disclosed. A scattering media is located within a beam path defined by the laser capture microdissection microscope and the sample is imaged through the scattering media. A transfer film carrier includes a substrate surface and a laser capture microdissection transfer...

20060148072 - Bioreactor system: A bioreactor system. A supply container receives a cultivation solution. A bioreactor container is connected to the supply container and includes a porous carrier. The porous carrier carries a plurality of cells. The cultivation solution circulates between the supply container and the bioreactor container, providing needed nutrients to the cells....

20060148071 - Covering device: The invention relates to a covering construction in a flexible device for covering heaped or stacked material, in particular for the aerobic treatment of organic-containing waste, for example residual waste or domestic refuse, in a compost heap. The covering construction has a cover which comprises a number of support elements...

20060148073 - Enhancement of adenoviral oncolytic activity in prostate cells by modification of the e1a gene product: The present invention relates to compositions and methods for enhancing the oncolytic activity of replication-competent, target cell-specific adenovirus vectors by modification of the E1A gene product. The target cell-specific replication-competent adenovirus vectors comprise a chimera of an adenovirus gene essential for replication, preferably an early gene, and the Androgen receptor...

20060148075 - Compositions, methods and systems for preparation of a stem cell-enriched cell population: In accordance with some preferred embodiments, without limitation, the present invention comprises compositions, methods and systems for preparation of stem-cell enriched cell populations from sources of biological materials by sorting cell types in relation to size....

20060148074 - Serum-free mammalian cell culture medium, and uses thereof: The present invention provides a cell culture medium formulation that supports the in vitro cultivation, particularly in suspension, of mammalian cells, particularly epithelial cells and fibroblast cells, and methods for cultivating mammalian cells in suspension in vitro using these media. The media comprise a basal medium and a polyanionic or...

20060148076 - Secreted and transmembrane polypeptides and nucleic acids encoding the same: The present invention is directed to novel polypeptides and to nucleic acid molecules encoding those polypeptides. Also provided herein are vectors and host cells comprising those nucleic acid sequences, chimeric polypeptide molecules comprising the polypeptides of the present invention fused to heterologous polypeptide sequences, antibodies which bind to the polypeptides...

20060148077 - Chondrocyte precursors derived from human embryonic stem cells: This invention provides a system for obtaining cells of the chondrocyte lineage by differentiating primate pluripotent stem cells. The process involves culturing the cells as a micromass or other aggregate form in a cocktail of differentiation agents that facilitates outgrowth of the desired cell type. Progeny are capable of synthesizing...

20060148081 - Expansion of definitive endoderm cells: Disclosed herein are cell cultures comprising expanded definitive endoderm cells as well as methods for expanding definitive endoderm cells in culture....

20060148079 - Isolation and purification of human insulin producing cells for the treatment of insulin dependent diabetes: A method for producing an ex vivo population of human insulin-producing cells that produce insulin in response to glucose from a patient is disclosed. The population of insulin producing cells is derived from human epithelial cells by causing those epithelial cells to differentiate into insulin producing cells. In one embodiment,...

20060148078 - Method of dynamically culturing embryonic stem cells: he present invention is of a method of dynamically generating human embryoid bodies which can be used for generating lineage specific cells and cell lines. Specifically, the present invention can be used to generate ESC-differentiated cells for cell-replacement therapy....

20060148080 - Methods for supporting and producing human cells and tissues in non-human mammal hosts: Provided are methods for culturing and producing human cell containing compositions, such as organs and blood, in non-human mammal hosts and for the preparation of animal-hosted human cell containing compositions for transplantation to human subjects. In one aspect, non-human hosts genetically modified or treated to reduce the expression of xenoantigens,...

20060148083 - Differentiation of brain tissue: A method is described for generating a clinically significant volume of neural progenitor cells from whole bone marrow. A mass of bone marrow cells may be grown in a culture supplemented with fibroblast growth factor-2 (FGF-2) and epidermal growth factor (EGF). Further methods of the present invention are directed to...

20060148084 - Effect of growth hormone and igf-1 on neural stem cells: The present invention provides a method of increasing neural stem cell numbers by using growth hormone and/or IGF-1. The method can be practiced in vivo to obtain more neural stem cells in situ, which can in turn produce more neurons or glial cells to compensate for lost or dysfunctional neural...

20060148082 - Method for delivering a biological compound using neural progenitor cells derived from whole bone marrow: A method is described for generating a clinically significant volume of neural progenitor cells from whole bone marrow. A mass of bone marrow cells may be grown in a culture supplemented with fibroblast growth factor-2 (FGF-2) and epidermal growth factor (EGF). Further methods of the present invention are directed to...

20060148085 - High yield heterologous expression cell lines for expression of gene products with human glycosylation pattern: The invention relates to ubiquitous/universal processes for establishing cells capable of stable high yield expression of a recombinant gene with human glycosylation pattern, and for establishing stable universal precursor cells available for insertion of arbitrary target genes. The invention further relates to cells obtainable by said processes...

20060148086 - Vault and vault-like carrier molecules: A method of using vaults as carrier molecules to deliver one or more than one substance to an organism, or to a specific tissue or to specific cells, or to an environmental medium. A vault-like particle. A method of preventing damage by one or more than one substance to an...

20060148087 - Development of a preventive vaccine for filovirus infection in primates: The present invention relates generally to viral vaccines and, more particularly, to filovirus vaccines and methods of eliciting an immune response against a filovirus or disease caused by infection with filovirus....

20060148088 - Fungi non-ltr retrotransposons and methods related thereto: Retrotransposons and methods related thereto. The retrotransposon comprises a nucleotide sequence selected from a group consisting of a nucleotide sequence of SEQ ID NO: 1, a nucleotide sequence of SEQ ID NO: 2, a nucleotide sequence of SEQ ID NO: 3, and a nucleotide sequence encoding a polypeptide of an...