Chemistry: molecular biology and microbiology inventions 06/06

20060141444 - Adherent entities and uses therefor: The present invention is directed to reproductive function-modulating organisms especially of the genera Ureaplasma and Mycoplasma, which adhere more strongly to spermatozoa than other organisms and which correlate with the presence or risk of a condition selected from male infertility, an adverse pregnancy outcome or an adverse assisted reproductive technology...

20060141443 - Apparatus and method for transcellular testing: The present invention relates to a device and method for facilitating high throughput transcellular flux testing of compounds, such as pharmaceuticals or drugs, other compounds, or compound combinations. In one embodiment, the system and methods of the present invention may be used to identify the optimal components (e.g., solvents, carriers,...

20060141447 - Cell culture media for mammalian cells: A chemically defined mammalian cell culture medium is provided that supports maintenance and long term clonal growth of mammalian hepatocytes and other cells....

20060141445 - Method for manufacturing a sensor chip and sensor chip blank: In a process for manufacturing a sensor chip a support is prepared having at least one surface region in which at least one reactive group is arranged. A buffer solution is applied to the surface region for coating at least one surface region. Then, at least one receptor having binding...

20060141446 - Substrates, devices, and methods for cellular assays: The present invention relates to the field of molecular diagnostics, and in particular to diagnostics based on a liquid crystal assay format. In particular, the present invention provided improved substrates and methods of using liquid crystal assays for quantitating the amount of an analyte in a sample. The present invention...

20060141448 - Whole cell assay: This invention relates to newly developed methods for discovering therapeutic compounds using a cell-based assay system. This invention also relates to compositions of matter useful in carrying out the methods of the invention as well as therapeutic compounds developed using such methods....

20060141449 - Yeast screens for treatment of human disease: Screening methods for identifying substances that provide therapeutic value for various diseases associated with protein misfolding are provided. Genetic and chemical screening methods are provided using a yeast system. The methods of the invention provide a rapid and cost-effective method to screen for compounds that prevent protein misfolding and/or protein...

20060141450 - Magnetism based rapid cell separation: This invention relates generally to the field of. In particular, the invention provides processes and kits for isolating a target cell, cellular organelle or virus from a sample, using inter alia, non- or low-specific binding between a target cell, cellular organelle or virus with a magnetic microbead....

20060141457 - Acyltransferase: The invention relates to at least one nucleotide sequence, derived from a nucleotide sequence encoding an acyltransferase polypeptide comprising at least one membrane-spanning region, encoding an improved active membrane independent acyltransferase polypeptide in which at least one amino acid residue of the membrane-spanning region has been deleted and/or substituted as...

20060141521 - Apparatus, methods and compositions for biotechnical separations: RNA, preferably messenger RNA, is purified by use of selective precipitation, preferably by addition of compaction agents. Also included is a scaleable method for the liquid-phase separation of DNA from RNA. RNA may also be recovered by fractional precipitation according to the invention. We have discovered that specific classes of...

20060141471 - Assay for detection of renal cell carcinoma: Methods for diagnosing and monitoring renal cell carcinoma in a subject comprising detecting kallikrein polypeptides, or polynucleotides encoding the polypeptides in a sample from the subject. The kallikrein polypeptides include kallikrein 5, kallirekin 6, kallikrein 10, and kallikrein 11....

20060141460 - Assays for fprl-1 ligands: Assays for screening for compounds that act as agonists, antagonists, or inverse agonists of CKβ8-1 at the FPRL-1 receptor....

20060141493 - Atherosclerotic phenotype determinative genes and methods for using the same: Genes whose expression is correlated with and determinant of an atherosclerotic phenotype are provided. Genes whose expression is correlated with and determinant of an atherosclerotic susceptibility are also provided. Also provided are methods of using the subject atherosclerotic determinant genes or the atherosclerotic susceptibility genes in diagnosis and treatment methods,...

20060141474 - Biomolecule detecting element and method for analyzing nucleic acid using the same: An inexpensive DNA chip/DNA microarray system capable of highly accurate measurement with low running cost. A DNA probe 8 is immobilized on a floating electrode 7 connected to a gate electrode 5 of a field effect transistor. Hybridization with a target gene is carried out on the surface of the...

20060141508 - Cellular signaling pathway based assays, reagents and kits: The present invention is directed to an assay system, PAM (Pathway Assay Matrix) for screening drug to determine whether they are effective for treating or preventing a disease or disorder. This assay system takes into account that many diseases involve complex pathways, and measures the effect of a particular drug...

20060141511 - Chemical amplification for the synthesis of patterned arrays: Radiation-activated catalysts (RACs), autocatalytic reactions, and protective groups are employed to achieve a highly sensitive, high resolution, radiation directed combinatorial synthesis of pattern arrays of diverse polymers. When irradiated, RACs produce catalysts that can react with enhancers, such as those involved in autocatalytic reactions. The autocatalytic reactions produce at least...

20060141505 - Chimeric fusion molecule for analyte detection and quantitation: Detector fusion molecules are produced by attaching a protein sub-unit to a linker, and attaching the linker to a nucleic acid molecule. The detector fusion molecules have utility in detecting and quantifying a specific target analyte from a sample. The protein sub-unit of the detector fusion molecule is selected to...

20060141470 - Chlamydia pneumoniae associated chronic intraocular disorders and treatment thereof: Infection by Chlamydia pneumoniae has been identified as a risk factor associated with the pathogenesis of certain chronic intraocular disorders, for example, age-related macular degeneration. The invention provides methods of identifying individuals at risk of developing chronic intraocular disorders, and methods of delaying and/or preventing the onset of such chronic...

20060141479 - Cngh0010 specific polynucleotides, polypeptides, antibodies, compositions, methods and uses: Novel polypeptides (CNGH0010) and antibodies, including specified portions or variants, specific for at least one such CNGH0010 polypeptide, variant, or fragment thereof, as well as nucleic acids encoding such CNGH0010 polypeptides and antibodies, complementary nucleic acids, vectors, host cells, and methods of making and using thereof, are useful for therapeutic...

20060141519 - Compositions and methods for diagnosing autism: Mutations located within the gene encoding the homeobox transcription factor, ENGRAILED 2 (EN2), have now been identified as molecular markers associated with susceptibility for autism and related disorders. Thus, the present invention relates to compositions in the form of diagnostic kits, primers and target sequences, for use in methods for...

20060141491 - Compositions and methods for enhancing polynucleotide amplification reactions: Compositions and methods for enhancing PCR and other polynucleotide replication reactions are disclosed. These comprise the addition of low molecular weight organic amides, sulfones or sulfoxides to PCR or other replication reaction mixtures....

20060141516 - De-novo sequencing of nucleic acids: The present invention is directed to an improved analysis procedure for the comparative sequencing of nucleic acids using multistage mass spectrometry. More precisely, the invention is directed to a method enabling the de-novo sequencing of nucleic acid molecules using multistage mass spectrometry....

20060141518 - Detection of gene expression: Methods for detection of nucleic acids such as a cDNA copy of an mRNA are disclosed. The methods comprise using a PCR to form a preamplification product which comprises cDNA sequence as well as primer target sequences and a detection probe sequence, which are introduced by the forward and reverse...

20060141503 - Detection of sequence variation of nucleic acid by shifted termination analysis: The invention relates to a method for detecting any mutation at a predetermined site occurring in a known nucleic acid sequence. The method uses primer extension analysis to detect the mutation. Unlabeled terminator is supplied along with labeled non-terminator in the primer extension reaction to detect whether the first nucleic...

20060141459 - Diagnostic and therapeutic use of foap-13 polynucleotides and polypeptides for neurodegenerative diseases: The present invention discloses the dysregulation of foap-13 gene expression in specific brain regions of Alzheimer's disease patients. Based on this finding, the invention provides a method for diagnosing or prognosticating Alzheimer's disease in a subject, or for determining whether a subject is at increased risk of developing Alzheimer's disease....

20060141485 - Electrically active combinatorial chemical (eacc) chip for biochemical analyte detection: Apparatus and methods are disclosed for electrically active combinatorial-chemical (EACC) chips for biochemical analyte detection. An apparatus includes a substrate that has an array of regions defining multiple cells, wherein each of the cells includes a reaction cavity that contains multiple functional binding groups. A method of detecting an analyte...

20060141473 - Erk7 and erk8, novel diagnostic markers for cancer: The present invention is directed in part to the discovery of a novel signal transduction pathway that regulates estrogen responsiveness. Human extracellular signal-regulated kinase 8 (ERK8) has been discovered by applicants to preferentially enhance the destruction of ERα, and loss of ERK8 is correlated with breast cancer progression. Thus monitoring...

20060141492 - Gene probes for the selective detection of microorganisms that reductively dechlorinate polychlorinated biphenyl compounds: The present invention relates to an assay for identification of PCB dechlorinating organisms that are capable of biologically removing PCBs from contaminated materials. Specifically, the invention provides a set of primers for detecting PCB dechlorinating organisms in a sample. These individual primers of the primer set have a sequence of...

20060141455 - Gene products differentially expressed in cancerous breast cells and their methods of use: The present invention provides polynucleotides, as well as polypeptides encoded thereby, that are differentially expressed in breast cancer cells. These polynucleotides are useful in a variety of diagnostic and therapeutic methods. The present invention further provides methods of reducing growth of breast cancer cells. These methods are useful for treating...

20060141476 - Growth arrest homeobox gene: A novel growth arrest homeobox gene has been discovered and the nucleotide sequences have been determined in both the rat and the human. The expression of the novel homeobox gene inhibits vascular smooth muscle cell growth. The growth arrest homeobox gene hereinafter referred to as the “Gax gene” and its...

20060141451 - Guinea pig proteinase-activated receptor 4 and its activating peptide: This invention relates to nucleic acid encoding guinea pig PAR 4 and to the protein encoded thereby. The guinea pig DNA and protein are useful for the development of models of human platelet aggregation. The invention further relates to an animal model to assess the role of PAR antagonists in...

20060141522 - Heregulin-like factor: The present invention relates to a novel HLF protein which is a member of the heregulin family. In particular, isolated nucleic acid molecules are provided encoding the human HLF protein. HLF polypeptides are also provided as are vectors, host cells and recombinant methods for producing the same. The invention further...

20060141481 - Hsv-1 and hsv-2 primers and probes: The present invention provides methods, primers and probes for the detection of HSV nucleic acids in biological fluids and tissue. In the methods of the invention, at least a portion of HSV nucleic acid present in a biological sample suspected of containing an HSV-1 and/or HSV-2 is amplified and the...

20060141462 - Human type ii diabetes gene-slit-3 located on chromosome 5q35: Association of Type II diabetes and a locus on chromosome 5q35 is disclosed. In particular, the gene SLIT-3 with this locus is shown by linkage analysis to be a susceptibility gene for Type II diabetes. Pathway targeting for drug delivery and diagnosis applications in identifying those have Type II diabetes...

20060141523 - Integrated method for pcr cleanup and oligonucleotide removal: A method is provided for purifying a desired polynucleotide product by removing unincorporated oligonucleotides from a polymerase or ligase reaction mixture....

20060141501 - Iterative probe design and detailed expression profiling with flexible in-situ synthesis arrays: Methods and compositions are provided that are useful for detecting and reporting a plurality of different target polynucleotide sequences in a sample, such as polynucleotides corresponding to a plurality of different genes expressed by a cell or cells. In particular, the invention provides methods for screening a plurality of candidate...

20060141494 - Luminescence detection apparatus: An object of the present invention is to provide a luminescence detection apparatus compact in size which is capable of conveniently determining DNA base sequences at a low cost. According to the present invention, a luminescence detection apparatus 1 is provided comprising: a plurality of reaction cells 6 each having...

20060141507 - Manufacture and use of non-standard size microarray slides: Methods and devices are disclosed for microarray analysis. In one embodiment a method is disclosed for processing a non-standard size slide having an array of chemical compounds attached to a surface of the slide. A sample is exposed to the surface of the non-standard size slide wherein components in the...

20060141472 - Medical use of ras antagonists for the treatment of capillary malformation: The invention relates to the field of vascular anomalies and methods for diagnosing and treating them. The invention provides for the causative gene (RASA1) and mutations therein which are useful for diagnosis of inherited capillary malformations. The invention further provides RASA1 antagonists for use in treatment of capillary malformations....

20060141482 - Method and multicomponent conjugates for treating cancer: The present invention provides a method for treating cancer in a mammal and to multicomponent conjugates useful in such treatment. In particular, it provides a cancer treatment method that includes administering multicomponent conjugates of formula: A-B-C, where “B” may be present or absent, “A” comprises at least one specific anti-cancer...

20060141466 - Method and sensor for detecting a chemical substance using an optically anisotropic material: A method and sensor for detecting a chemical substance in an analyte. An anisotropic material is subjected to the analyte. Light is passed through the anisotropic material and collected. A change of an optical anisotropy of the collected light is detected, the change being indicative of the chemical substance in...

20060141464 - Method for immobilizing biologic molecules on solid surfaces: The invention provides a method for immobilization of biological molecules such as nucleic acids, peptides and proteins onto the surface of a glass or plastic solid support....

20060141510 - Method for information processing with nucleic acid molecules: The present invention is directed to provide an information processing method using autonomously workable nucleic acids, and a molecular computer to carry out operations with the method. Objectives above mentioned may be achieved with following method. The present invention provides an information processing method carrying out operations with functions receiving...

20060141513 - Method for recognition and recovery of cis-regulatory modules: A computational method is described which uses the process of cis-regulatory module evolution to identify conserved sequence patches which exhibit suppression of change by snp/indel occurrence, in the absence of having to execute multiple interspecific sequence comparison analysis, including libraries, and arrays that contain such cis-regulatory modules....

20060141488 - Method for stabilizing or preserving nucleic acid by using amino surfactants: A method for stabilizing or persevering nucleic acids by forming an insoluble ionic complex between nucleic acids and a surfactant in a biological sample, consisting of a step of contacting the biological sample with an isolation reagent comprising amino surfactants of the formula (I): R1R2R3N(O)x, (I), wherein, R1 and R2...

20060141452 - Method for synthesizing single-stranded nucleic acid: This invention relates to a method for selectively and efficiently synthesizing one of the sense or antisense strands of double-stranded nucleic acid. The method for synthesizing single-stranded nucleic acid according to this invention comprises the following steps of: 1) cleaving, with a restriction enzyme, double-stranded DNA having a restriction enzyme...

20060141463 - Method of detecting bone paget's disease: A method for detecting Paget disease of bone and an animal showing the pathology of Paget disease of bone are provided. In particular, there are provided associating Paget disease of bone with the mutation a chondroitin/chondroitin synthase gene comprising the amino acid sequence depicted in any one of SEQ ID...

20060141458 - Method of identifying genes involved in cell growth regulation: A method is provided for identifying in eukaryotes nucleic acid sequences that are involved in or suspected of being involved in cell growth regulation comprising mutagenising wild type plants or plants genetically modified to have accelerated tissue growth and selecting mutants having relative to the background and altered phenotype, followed...

20060141465 - Method of immobilizing a polynucleotide on a solid support, solid immobilization support thus obtained, method of detecting target polynucleotide from the solid support and solution for immobilizing the polynucleotide: It is intended to provide a method of immobilizing a polynucleotide on a solid support by spotting the polynucleotide on the solid support in a uniform spot shape without any irregularity. Namely, a method of immobilizing a polynucleotide on a solid support by spotting a solution containing the polynucleotide together...

20060141496 - Method of screening substance interacting with abc protein: The present invention relates to a method for measuring transporter activity of ABC protein that may measure not only small amount of samples with small data fluctuation and good reproducibility, but also many samples simultaneously and concisely; a screening method for substances that interact with ABC protein using said measuring...

20060141489 - Method of statistical genomic analysis: A method of design and analysis of microarray studies has been developed. The method includes classification of the microarrays; measurement of the reliability and validity of expression measurements; estimation of the effect of an experimental manipulation on gene expression; design of a statistical analysis to determine the distinction between biological...

20060141478 - Methods and compositions for detecting and treating genetically induced chronic diseases: The present invention discloses a functional relationship between a recognized disease condition and a polymorphism in the nucleotide factor kappa B promoter (NFKB1). This relationship provides a platform for methods of altering promoter activity and for determining similar relationships between specific pathologies and identified polymorphisms. A statistically significant risk of...

20060141456 - Methods and compositions for milieu-dependent binding of a targeted agent to a target: The present invention provides methods and compositions for milieu-dependent binding of a targeted agent to a target, for example, for the milieu-dependent binding of a diagnostic or therapeutic molecule to a diseased, injured or infected organ, tissue or cell....

20060141502 - Methods and compositions for the preparation and use of fixed-treated cell-lines and tissue in fluorescence in situ hybridization: This invention relates to methods for the detection of one or more mRNA transcripts in paraffin-embedded tissue by “mRNA liberation in fixed-treated tissue or ‘MLIFTT’”. This method includes treating the tissue with ammonia-ethanol and sodium borohydride combined with pressure cooking of the tissue. The chemical treatments reduce the tissue autofluorescence...

20060141500 - Methods and compounds for the diagnosis of inflammatory disease and identification of pharmacological agents useful in the treatment of inflammatory disease: Methods for the diagnosis of inflammatory bowel diseases and the identification of agents useful in the treatment of such diseases based upon the agent's effect on reducing Pim-2 expression....

20060141467 - Methods and kits for making double stranded ribonucleic acids: Embodiments of the present invention are directed to methods and kits for the generation of double stranded RNA by RNA dependent RNA polymerases. The methods and kits feature exponential generation of such RNAs through simple steps/ The RNA is suitable for interference with expression in cells....

20060141498 - Methods for fragmenting nucleic acid: Methods for using an apurinic/apyrimidinic endonuclease, capable of cleaving both single- and double-stranded cDNA, for fragmentation and labeling of single stranded or double stranded DNA molecules are provided. Amplification methods that generate single-stranded amplified cDNA are also disclosed. In the subject methods AP sites in a population of nucleic acids...

20060141506 - Methods for monitoring the expression of alternatively spliced genes: Methods, probe arrays and computer software products are provided for determining the arrangement of sequence elements. In one embodiment, methods for making and using exon chips are provided. The exon chips may be used to identify and quantify splice variants....

20060141520 - Methods for the treatment of metabolic disorders, including obesity and diabetes: The present invention relates to methods and compositions for the diagnosis and treatment of metabolic disorders, including, but not limited to, obesity, diabetes, overweight, anorexia, or cachexia. The invention further provides methods for identifying a compound capable of treating a metabolic disorder. The invention also provides methods for identifying a...

20060141461 - Methods of assessing the risk of reproductive failure by measuring telomere length: The invention features a method of identifying oocytes with a risk of reproductive failure and/or aneuploidy based on a telomere length assay....

20060141499 - Methods of determining human egg competency: The inventors have discovered inter alia, that when the chromosome complement of a first polar body (PB-I) is normal, it alone is in more than 80% of cases sufficient to predict that an embryo which has developed from an oocyte associated with the PB-I is also normal and competent. As...

20060141487 - Methods, compositions, and kits for amplifying and sequencing polynucleotides: In one aspect, there are provided methods of amplifying and sequencing a polynucleotide. In some embodiments, the method includes (a) amplifying the polynucleotide with at least one amplification primer, a processive amplification polymerase, a sequencing primer, a sequencing polymerase, deoxynucleoside triphosphates suitable for template-dependent primer extension, and one or more...

20060141497 - Molecular analysis of cellular fluid and liquid cytology specimens for clinical diagnosis, characterization, and integration with microscopic pathology evaluation: The application relates to methods, materials, kits, and devices for characterizing fluid specimens of any type or source that may or may not contain cells. The methods, materials, kits, and devices can be used for analyzing fluid obtained from various organs or near a purported tumor site, such as via...

20060141504 - Molecular technologies for improved risk classification and therapy for acute lymphoblastic leukemia in children and adults: The present invention relates to methods for predicting the outcome of therapeutic intervention in cases of leukemia, especially acute lymphoblastic leukemia in children and adults. The present invention evaluates a gene expression profile and identifies prognostic genes of cancers, in particular leukemia, more particularly B-precursor acute lymphoblastic leukemia (ALL). The...

20060141469 - Multi-layered electrochemical microfluidic sensor comprising reagent on porous layer: The present invention relates to a microfluidic electrochemical sensor apparatus and a method for conducting analytical tests with said apparatus for multi-reactant assays. The apparatus of this invention is a multi-layer body made of at least three layers, the first one being a polymer layer (1) comprising a microstructure (5)...

20060141490 - Myd88 as a therapeutic target for cancer: The invention relates to methods for identifying anti-cancer agents using MyD88 as a therapeutic target....

20060141524 - Novel kinases and uses thereof: Novel kinase polypeptides, proteins, and nucleic acid molecules are disclosed. In addition to isolated, full-length kinase proteins, the invention further provides isolated kinase fusion proteins, antigenic peptides, and anti-kinase antibodies. The invention also provides kinase nucleic acid molecules, recombinant expression vectors containing a nucleic acid molecule of the invention, host...

20060141512 - Novel method for separation of human sperm from biological samples for application in human identification: The development of an isolation methodology for separation of human sperm cells from biological samples containing human epithelial cells is provided. Using sperm binding proteins, glycopeptides, lectins, derivatives of N-acetylglucosamine, triazine dyes or inhibitors of glycosyltransferase linked to an insoluble support, our invention enables binding of human sperm cells from...

20060141468 - Novel notch-like polypeptides: The present invention discloses open reading frames (ORFs) in human genome encoding for novel notch-like polypeptides, and reagents related thereto including variants, mutants and fragments of said polypeptides, as well as ligands and antagonists directed against them. The invention provides methods for identifying and making these molecules, for preparing pharmaceutical...

20060141495 - Polymorphic markers and methods of genotyping corn: Polymorphic corn DNA loci useful for genotyping between at least two varieties of corn. Sequences of the loci are useful for designing primers and probe oligonucleotides for detecting polymorphisms in corn DNA. Polymorphisms are useful for genotyping applications in corn. The polymorphic markers are useful to establish marker/trait associations, e.g....

20060141475 - Polynucleotide sequence detection assays and analysis: Methods and software for associating mobility probes with target macromolecules are discussed. By encoding the identities of macromolecules of interest with a universal set of tag portions complementary to a universal set of mobility probes, reactions varying in their input starting material may be identified using the same universal set...

20060141515 - Polynucleotides encoding plant cysteine proteases: This invention relates to an isolated nucleic acid fragment encoding a proteinase. The invention also relates to the construction of a chimeric gene encoding all or a portion of the proteinase, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the...

20060141486 - Porous substrates and arrays comprising the same: The present invention relates to arrays comprising porous substrates for attachment of nucleic acids, polypeptides, membranes, or other biological or organic materials. In many embodiments, the arrays of the present invention have a flow-through configuration such that washing buffers or samples can access to the porous substrates from at least...

20060141453 - Prokineticin polypeptides, related compositions and methods: The invention provides isolated polypeptides that stimulate gastroeintestinal smooth muscle contraction, including human prokineticin 1 and human prokineticin 2 polypeptides, and functional fragments and modifications thereof. Also provided are methods of stimulating gastrointestinal smooth muscle contraction in a mammal, by administering to the mammal an effective amount of a prokineticin...

20060141517 - Protein having pdz domain sequence: While analyzing changes in gene expression by TNFα in human umbilical vascular endothelial cells, a gene showing enhanced expression due to stimulation with TNFα was isolated. After screening with this gene as a probe, a gene encoding a protein was isolated. Analysis of the protein encoded by this isolated gene...

20060141484 - Reactive ion etched substrates and methods of making and using: Disclosed herein are substrates comprising reactive ion etched surfaces and specific binding agents immobilized thereon. The substrates may be used in methods and devices for assaying or isolating analytes in a sample. Also disclosed are methods of making the reactive ion etched surfaces....

20060141509 - Recovery of virus from cell culture using a hypertonic salt solution: A process of harvesting a herpesvirus from a cell culture infected therewith comprises treating said culture with a hypertonic aqueous salt solution to yield a virus suspension, e.g. to give improved yield of virus for live virus vaccine where otherwise cell-disruption might be used to harvest the virus by disrupting...

20060141483 - Stabilization of viral compositions: This invention concerns stabilized virus compositions, preferably a herpesvirus which may be an attenuated or genetically modified herpes simplex virus or varicella zoster virus, and a method of stabilizing viruses and immunizing preparations by the addition of sugars, preferably glucose and amino acids, preferably lysine....

20060141514 - Thermus brockianus nucleic acid polymerases: The invention provides nucleic acids and polypeptides for nucleic acid polymerases from a thermophilic organism, Thermus brockianus. The invention also provides methods for using these nucleic acids and polypeptides....

20060141480 - Use of computationally derived protein structures of genetic polymorphisms in pharmacogenomics and clinical applications: Provided herein are computer-based methods for generating and using three-dimensional (3-D) structural models of target molecules and databases containing the models. The targets can be protein structural variants derived from genes containing polymorphisms. The models are generated using molecular modeling techniques and are used in structure-based drug design studies for...

20060141477 - Use of parvovirus capsid particles in the inhibition of cell proliferation and migration: The invention described herein relates to the discovery of methods and compositions for the inhibition of growth and/or migration of cells that have a receptor that interacts with a parvovirus B19 capsid or fragment thereof (e.g., a P antigen containing cell), including but not limited to, cells of hematopoietic origin...

20060141454 - Use of the slug gene, or of the transcription or expression products thereof in the detection and/or treatment of cancerous cells: Method for the detection of cancerous cells, in particular, mesenchymal tumoral cells, in a biological sample suspected of containing said malignant cells. The methods are based on the aberrant expression of the Slug gene or transcription or expression products thereof. Also disclosed are methods of treatment that based on the...

20060141528 - Compositions and methods for quantification of serum glycoproteins: The invention provides compositions and methods for identifying and/or quantifying glycopolypeptides from human serum or plasma. The compositions and methods include a plurality of standard peptides containing glycosylation sites determined for human serum/plasma proteins....

20060141529 - Compositions, kits and assays containing reagents directed to cortactin and an arg/abl protein kinase: It has been determined Cortactin interacts with an ARG/ABL protein kinase. Thus, provided herein are compositions, kits and assays containing reagents directed to Cortactin and an ARG/ABL protein kinase....

20060141537 - Device and method for separating molecules: An apparatus and method for separating a biomolecule from one or more contaminants in a sample. The apparatus can include a unitary device comprising a plurality of fractionation devices, each reservoir comprising a filter, each reservoir adapted to contain a solid phase, the solid phase adapted to separate the biomolecule...

20060141526 - Genetic marker for coronary artery disease: The present invention describes a Coronary artery disease (CAD) susceptibility gene and the use of this gene in the development of novel therapies to treat CAD....

20060141535 - Hyaluronan receptor for endocytosis: A purified nucleic acid segment encoding a functionally active hyaluronan receptor for endocytosis (HARE) or an active peptide fragment thereof, and methods for producing functionally active HARE or an active peptide fragment thereof therefrom, wherein the functionally active HARE or an active peptide fragment thereof is able to specifically bind...

20060141527 - Method for creating a reference region and a sample region on a biosensor and the resulting biosensor: A method is described herein that can use any one of a number of deposition techniques to create a reference region and a sample region on a single biosensor which in the preferred embodiment is located within a single well of a microplate. The deposition techniques that can be used...

20060141532 - Method for identifying and designing immunogenic peptides: The invention provides a method for identifying peptides for use in increasing a cytotoxic T lymphocytes (CTL) response to an antigen. The method comprises the steps of comparing the amino acid sequence of the VH and/or VL portions of an anti-idiotypic monoclonal antibody to the amino acid sequence of an...

20060141533 - Rapid identification of conditions, compounds, or compositions that inhibit, prevent, induce, modify, or reverse transitions of physical state: The invention concerns arrays comprising hundreds, thousands, to hundreds of thousands of samples and methods for screening thereof. These methods are useful to optimize, select, and discover compounds, compositions, or conditions that prevent, inhibit, induce, modify, or reverse physical-state transitions, particularly in-vivo physical-state transitions relating to disease causing processes. Such...

20060141530 - Reagents and procedures for multi-label high-specificity labeling: A detectable complex and methods for use thereof are provided herein. The detectable complex includes: at least one target material; a first peptide tag bound to the at least one target material; and a second peptide tag bound to the at least one target material. The complex further includes a...

20060141536 - Scintillation proximity test: The invention concerns scintillation proximity assays performed in multiwell plates where a charge coupled device is used to image the wells. Conventional phosphors emit blue light (350-450 nm) which is absorbed by yellow or brown assay components. This problem is addressed by the use of phosphors that emit radiation of...

20060141531 - Ultra-high specificity fluorescent labeling: A self-assembled relay probe for detecting a target material is provided including: a first peptide tag bound to the target material; and a first fluorescent conjugate including a first fluorochrome and a first tag binding group; wherein the first fluorescent conjugate selectively associates with the first tag. The probe further...

20060141525 - Use of lectin library for distinguishing glycoproteins or cells, diagnosing serum or cells, or fractionating glycoproteins or cells: It is intended to provide lectins and a lectin library comprising these lectins which are useful in recognizing differences in carbohydrates on cell surface (for example, sugar chains and cell surface glycoproteins) reflecting extremely subtle differences among cells, etc. and thus distinguishing, identifying or fractionating various cells....

20060141538 - Automatic large-scale screening of cells secreting monoclonal antibodies: The invention relates to an automatic method for the large-scale in vitro screening of cells, secreting at least one specific monoclonal antibody with affinity for a compound of interest, comprising: (10) distribution of antibody-producing cells on at least one culture plate, (12) culturing of said cells, (14) iterative screening of...

20060141540 - Direct selection of cells by secretion product: Cells can be labeled with products which they secrete and release in an efficient manner by coupling the cells at their surface to a specific binding partner for the product and allowing the product to be captured by the specific binding partner as it is secreted and released. The product-labeled...

20060141541 - Method of detecting or diagnosing of a neurodegenerative disease or condition: A neurodegenerative disease or condition is diagnosed in a subject by obtaining a sample of cerebral spinal fluid from the subject and assaying the sample by an assay method that detects the presence of at least one antiphospholipid autoantibody in the sample, wherein an elevated level of at least one...

20060141539 - Miniaturized cell array methods and apparatus for cell-based screening: The present invention discloses devices and methods of performing high throughput screening of the physiological response of cells to biologically active compounds and methods of combining high-throughput with high-content spatial information at the cellular and subcellular level as well as temporal information about changes in physiological, biochemical and molecular activities....

20060141545 - Cancer treatment kits using antibodies to aminophospholipids: Disclosed are the surprising discoveries that aminophospholipids, such as phosphatidylserine and phosphatidylethanolamine, are stable and specific markers accessible on the luminal surface of tumor blood vessels, and that the administration of an anti-aminophospholipid antibody alone is sufficient to induce thrombosis, tumor necrosis and tumor regression in vivo. This invention therefore...

20060141543 - Cellular retinoic acid binding protein ii as a marker for breast cancer: The present invention relates to the diagnosis of breast cancer. It discloses the use of protein cellular retinoic acid binding protein II in the diagnosis of breast cancer. It relates to a method for diagnosis of breast cancer from a liquid sample, derived from an individual by measuring cellular retinoic...

20060141544 - Compositions for binding to assay substrata and methods of using: Compositions and methods for binding to assay substrata in a stable and protective manner, thereby enhancing assay performance, are provided. The compositions comprise lyotropic materials (for example, lyotropic liquid and/or liquid crystalline materials) and may contain macromolecular standards, markers or capture compounds. The compositions are capable of binding to assay...

20060141546 - Bacterial test method by glycated label binding: A method for measuring the bacteria content of fluids such as urine and blood, in which a glycoprotein or glycopeptide is attached to the bacteria and a label attached to or inherent to the glycoprotein or glycopeptide provides a means for determining the amount of bacteria present. A preferred glycoprotein...

20060141542 - Alpha-amylase assay and uses thereof: This invention relates to methods and kits for measuring α-amylase activity in samples such as flour, stock, or amylase concentrate....

20060141548 - Immunoassays for everolimus: Immunoassays for the detection of everolimus are provided. Compounds for producing antibodies for everolimus, as well as antibodies produced therefrom, are also provided....

20060141547 - Novel diagnostic marker, a diagnostic kit and a method for diagnosis of rheumatoid arthritis: The present invention relates to a novel diagnostic marker useful for the diagnosis of rheumatoid arthritis comprising the autoantibodies of mannose binding lectin protein and a process thereof....

20060141549 - Cell-based kinase assay: The present invention relates to improved systems and strategies for the investigation of kinase activity in cells. More specifically, cell-based assay methods are provided that allow the phosphorylating activity of a kinase to be determined inside a cell. The invention also provides cell-based screening assays for identifying compounds that have...

20060141550 - Novel metabolic targets and markers: The present invention provides metabolic markers and targets, especially PEMT activity and metabolic markers and components associated therewith useful for diagnosing and treating various disease conditions....

20060141551 - Analytical method for detecting alkaline sphingomyelinase and kit for use in such method: The use of an alkanoyl L-carnitine for preparing a medicament for treating the erectile dysfunction is described....

20060141552 - Regulation of human caspase-1-like protease: Screening methods to identify candidate therapeutic agents using a newly identified human caspases-1-like protease....

20060141553 - Process for preparing microbial consortium useful as seeding material for bod analysis of pulp and paper industrial wastewater: The present invention provides a seeding material for accurate and reproducible monitoring of BOD load of pulp and paper wastewater, wherein the bacteria are isolated from various locations in India and comprise Micrococcus sp., Staphylococcus sp., Kurthia zopfii, Alcaligenes faecalis, and Pseudomonas aeruginosa, the microbial composition being capable of exerting...

20060141554 - Site-specific labeling of affinity tags in fusion proteins: The present invention provides methods and fluorescent compounds that facilitate detecting and labeling of a fusion protein by being capable of selectively binding to an affinity tag. The fluorescent compounds have the general formula A(B)n, wherein A is a fluorophore, B is a binding domain that is a charged chemical...

20060141555 - Control of biocatalysis reactions: A process for increasing the rate of biocatalysis reactions, which comprises applying a direct current electric field to a reaction mixture, wherein the reaction mixture and the electrodes used to apply said electric field are separated such that the reaction mixture does not come into contact with said electrodes....

20060141556 - Microwave cell lysis method using ionic additives: A cell lysis method in which an ionic additive and microwaves are used is provided. The cell lysis method includes: preparing a sample containing cells to be lysed; adding an ionic additive to the sample; and lysing cells in the sample by radiating microwaves onto the sample in which the...

20060141558 - Bioproduction of astaxanthin using mutant carotenoid ketolase and carotenoid hydroxylase genes: Protein engineered nucleic acid fragments encoding a CrtO ketolase and a CrtZ hydroxylase are provided with increased astaxanthin synthesis activity. Methods using the present nucleic acid fragments are also provided for increasing or altering astaxanthin production in suitable production hosts....

20060141557 - Production of canthaxanthin by phaffia: Disclosed is a process for producing canthaxanthin and echinenone which comprises cultivating a recombinant microorganism which is expressing a β-casotene ketolase gene and belonging to the genus Xanthophyllomyces (Phaffia) in an aqueous nutrient medium under aerobic conditions, and isolating the resulted carotenoids from the cells of said recombinant microorganism or...

20060141559 - Extract from cultured mammalian cell, process for preparation thereof and method of cell-free protein synthesis using the extract: A method for preparing a cultured mammalian cell extract liquid, comprising at least the step of rapidly freezing a cultured mammalian cell suspended in a solution for extraction; a cultured mammalian cell extract liquid prepared by the process; and a method for cell-free protein synthesis using the extract liquid. Preferably,...

20060141561 - Apo-2 ligand/trail variants and uses thereof: The disclosure provides Apo-2 ligand variant polypeptides. Methods of making and chemically modifying Apo-2 ligand variant polypeptides are also provided. In addition, formulations of Apo-2 ligand variant polypeptides are provided. In addition, therapeutic methods for using Apo-2 ligand variant polypeptides are provided....

20060141569 - Compositions and methods for the assay of g-protein coupled receptors and their ligands: A cell comprising one or more endogenous promiscuous G-proteins and an exogenous nucleic acid sequence that encodes a G-protein coupled receptor (GPCR), which optionally comprises a calcium release activated calcium channel (CRAC channel) provides features and improvements that significantly improve membrane level expression of the GPCR, increase signal responses and...

20060141560 - Estrogen receptor genes and utilization thereof: An estrogen receptor gene comprising a nucleotide sequence coding for any of the following amino acid sequences: (a) the amino acid sequence of SEQ ID NO: 1, (b) the amino acid sequence of SEQ ID NO: 4, (c) the amino acid sequence of SEQ ID NO: 23, (d) an amino...

20060141567 - Human chemotactic cytokine: The invention relates to isolated and/or recombinant nucleic acids which encode a human chemotactic cytokine designated human eotaxin, and to isolated and/or recombinant human eotaxin proteins or polypeptides, including synthetic polypeptides. The invention further relates to recombinant nucleic acid constructs, comprising a nucleic acid which encodes a human eotaxin, a...

20060141570 - Intein-mediated protein purification using in vivo expression of an aggregator protein: Purification of recombinant proteins is performed by expressing in a host cell a fusion protein comprising: (a) a product protein domain, (b) an intein, and (c) at least one aggregator protein domain, wherein the aggregator protein domain comprises a protein that is capable of specific association with granules of polyhydroxyalkanoate...

20060141574 - Method for producing monoclonal antibodies: An improved method for the production of monoclonal antibodies is disclosed....

20060141571 - Method for promoting cell growth and increasing the production of the expressed target gene products: The present invention is related to a method to promote cell growth, comprising: (a) cloning one or more aspartase coding sequence into a vector to become a recombinant vector, wherein the recombinant vector including a promoter and one or more aspartase coding sequences manipulated to link and insert into the...

20060141568 - Modification of collagenous materials and medical treatment, diagnosis and monitoring of fibrotic conditions: The present invention relates to the gene PLOD2 which codes for telopeptide lysyl hydroxylase (TLH). This enzyme converts telopeptidyl Lys into telopeptidyl Hyl, that can subsequently be converted into hydroxyallysine cross-links. Collagen with hydroxyallysine cross-links shows a higher resistance to degradation by proteinases than collagen with cross-links derived from allysine....

20060141562 - Mutant e. coli appa phytase enzymes and natural variants thereof, nucleic acids encoding such phytase enzymes, vectors and host cells incorporating same and methods of making and using same: Cells producing mutant phytases having modified activity are provided, as well as the phytases so produced. Also provided are methods of making and producing such phytases and the use of the expressed phytase protein in feed as a supplement....

20060141563 - Mutant protein and refolding method: An FrpB protein having one or more deletions of non-conserved amino acids compared to a corresponding wild-type FrpB protein, and a method of refolding an FrpB protein....

20060141565 - Novel fibroblast growth factor and uses thereof: The present invention generally relates to nucleic acids, proteins, and antibodies. The invention relates more particularly to nucleic acid molecules, proteins, and antibodies of Fibroblast Growth Factor-20 (FGF-22), or its fragments, derivatives, variants, homologs, analogs, or a combination thereof....

20060141573 - Novel tumor necrosis factor receptor homolog and nucleic acids encoding the same: The present invention is directed to novel polypeptides having homology to members of the tumor necrosis factor receptor family and to nucleic acid molecules encoding those polypeptides. Also provided herein are vectors and host cells comprising those nucleic acid sequences, chimeric polypeptide molecules comprising the polypeptides of the present invention...

20060141564 - Nucleic acids encoding duramycin: Nucleic acids encoding preduramycin and produramycin are described, along with recombinant nucleic acids and host cells containing the same and methods of use thereof, such as for the manufacture of the lantibiotic duramycin....

20060141572 - Recombinant light chains of botulinum neurotoxins and light chain fusion proteins for use in research and clinical therapy: Botulinum neurotoxins, the most potent of all toxins, induce lethal neuromuscular paralysis by inhibiting exocytosis at the neuromuscular junction. The light chains (LC) of these dichain neurotoxins are a new class of zinc-endopeptidases that specifically cleave the synaptosomal proteins, SNAP-25, VAMP, or syntaxin at discrete sites. The present invention relates...

20060141575 - Secreted proteins and nucleic acids encoding them: The invention concerns cDNA molecules encoding TANGO 180, TANGO 181, TANGO 182, TANGO 183, TANGO 184, TANGO 185, TANGO 186, TANGO 187, TANGO 188, TANGO 189, and TANGO 215, all of which are either secreted or transmembrane proteins. The nucleic acids and polypeptides of the present invention are useful as...

20060141577 - Selection of host cells expressing protein at high levels: The invention provides a DNA molecule comprising a multicistronic transcription unit coding for i) a polypeptide of interest, and for ii) a selectable marker polypeptide functional in a eukaryotic host cell, wherein the polypeptide of interest has a translation initiation sequence separate from that of the selectable marker polypeptide, and...

20060141576 - Variants of corticotropin releasing hormone receptor type 1 and uses thereof: The present invention identifies four new isoforms of human corticotropin releasing hormone receptor type 1 (CRH-R1e, 1f, 1g and 1h) and three new isoforms of mouse corticotropin releasing hormone receptor type 1 (mCRH-R1c, 1e and 1f). The data indicate that polymorphism of CRH-R1 expression is related to anatomic location, skin...

20060141566 - Virus preparations and methods: Methods are disclosed for the preparation of herpesvirus, such as herpes simplex virus type 2 for vaccine use. Such viruses can be grown on serum free or serum containing media and can be prepared from the virus containing culture supernatant or virus containing cells. The virus is prepared for subsequent...

20060141578 - Method of protein synthesis: The present invention relates to a method of protein synthesis and, more particularly, to a method of effective protein synthesis by regulating the expression of a protein in a host cell, wherein said host cell is transformed with an expression vector comprising a promoter as well as a DNA fragment...

20060141579 - Cytokine zcytor17 ligand polynucleotides: The present invention relates to zcytor17lig polynucleotide, polypeptide and anti-zcytor17 antibody molecules. The zcytor17lig is a novel cytokine. The polypeptides may be used within methods for stimulating the immune system, and proliferation and/or development of hematopoietic cells in vitro and in vivo. The present invention also includes methods for producing...

20060141580 - Interferon-alpha induced gene: The present invention relates to identification of a gene upregulated by interferon-α administration corresponding to the cDNA sequence set forth in SEQ. ID. NO. 1. Determination of expression products of this gene is proposed as having utility in predicting responsiveness to treatment with interferon-α and other interferons which act at...

20060141581 - Il-7 variants with reduced immunogenicity: Modified interleukin-7 (IL-7) polypeptides are disclosed. The modified IL-7 polypeptides have alterations to one or more potential T-cell epitopes, thereby to reduce a T-cell response....

20060141582 - Anti-il-22ra antibodies and binding partners and methods of using in inflammation: The present invention relates to blocking, inhibiting, reduceing, antagonizing or neutralizing the activity of IL-22, IL-20, or both IL-20 and IL-22 polypeptide molecules. IL-20 and IL-22 are cytokines that are involved in inflammatory processes and human disease. IL-22RA (zcytor11) is a common receptor for IL-20 and IL-22. The present invention...

20060141583 - Elaiophylin biosynthetic gene cluster: The present invention relates especially to a DNA fragment that is obtainable from the gene cluster within the genome of streptomycete that is responsible for elaiophylin biosynthesis and that contains at least one gene or a part of a gene that codes for a polypeptide that is involved directly or...

20060141584 - Pretreatment of biomass for ethanol production: A pretreatment of biomass for ethanol production is disclosed. The pretreatment of biomass such as sawdust or wood chips by processing such biomass through an extruder results in a greatly increased recovery of sugar using hydrolysis....

20060141586 - Method for producing l-amino acids using bacteria of the enterobacteriaceae family: There is disclosed a method for producing an L-amino acid, for example L-threonine, L-lysine, L-histidine, L-phenylalanine, L-arginine, L-tryptophan or L-glutamic acid, using a bacterium of the Enterobacteriaceae family, wherein the bacterium has been modified to enhance an activity of L-arabinose permease....

20060141585 - Microorganisms and processes for enhanced production of pantothenate: The present invention features improved methods for the enhanced production of pantoate and pantothenate utilizing microorganisms having modified pantothenate biosynthetic enzyme activities and having modified methylenetetrahydrofolate (MTF) biosynthetic enzyme activities. In particular, the invention features methods for enhancing production of desired products by increasing levels of a key intermediate, ketopantoate,...

20060141587 - Process for the preparation of l-3, 4-dihydroxyphenylalanine by aerobic fermentation of a microorganism: The invention relates to a process for the preparation of L-3,4-dihydroxyphenylalanine wherein L-3,4,-dihydroxyphenylalanine is produced in a fermentation medium by aerobic fermentation of a recombinant microorganism having L-tyro-sine-3-hydroxy-mono-oxygenase activity and at least the metabolic pathways: glycolysis, pentose phosphate pathway, aromatic amino acid pathway, or derivative pathways thereof, which process comprises...

20060141588 - L-glutamic acid-producing microorganism and a method for producing l-glutamic acid: A coryneform bacterium that is modified by using a yggB gene so that L-glutamic acid-producing ability is enhanced as compared to a non-modified strains is cultured in a medium to cause accumulation of L-glutamic acid in the medium or bacterial cells, and L-glutamic acid is collected from the medium or...

20060141589 - Method of producing macrolide compound: The present invention provides a novel method of producing the 12-membered ring macrolide compound 11107D having an antitumor activity by biological transformation. Starting material which is the 12-membered ring macrolide compound 11107B represented by the formula (I) is incubated in the presence of a strain belonging to the genus Mortierella,...

20060141590 - Dna encoding favin-adenine-dinucleotide-dependent-d-erythronate-4-phosphate-de-hydrogenase, pdxr, and microbial production of vitamin b6: The present invention relates to a DNA encoding a novel flavin adenine dinucleotide (FAD)-dependent D-erythronate 4-phosphate (EN4P) dehydrogenase originated from Sinorhizobium meliloti, which is involved in vitamin B6 biosynthesis, and a recombinant microorganism transformed with a vector having the DNA. It also relates to a process for production of vitamin...

20060141591 - Method for producing optically active chroman-carboxylate: In the method for producing an optically active chromancarboxylate of the present invention, one of the enantiomers of racemic chromancarboxylic acid is esterified in a solvent containing an alcohol in the presence of a biocatalyst. After the esterification, the other enantiomer, i.e., the non-reacted chromancarboxylic acid is separated out of...

20060141592 - Process for production of transesterified oils/fats or triglycerides: There are provided polyunsaturated fatty acids-containing oils/fats or triglycerides with high digestion and absorption properties and resistance to oxidative damage, which are suitable for applications in fields such as modified milk for infants, food products and healthy foods and/or supplements, produced by transesterification of polyunsaturated fatty acids-containing oils/fats or triglycerides...

20060141593 - Enzymatic production of (meth) acrylic acid esters: A method for the production of partially esterified (meth)acrylic acid esters of polyalcohols and the use thereof....

20060141594 - Simultaneous anaerobic production of isoamyl acetate and succinic acid: In vivo method of producing esters from acetyle coA, such as isoamyl acetate and succinate, has been developed by producing null mutants in pathways that use acetyl coA and by overexpressing products that use NADH and in order to maintain the proper redox balance between NADH and NAD+. The method...

20060141595 - Process for continuous manufacture of invert sugar syrup and alcohol: The present invention relates to an improved process for the manufacture of invert sugar syrup and alcohol capable of operating in the continuous mode for enzymatic hydrolysis of sucrose or sucrose containing sources for the production of invert syrup and thereby alcohol fermentation and using a recyclable solid catalyst...

20060141596 - Process for the immobilization of enzymes: The present invention relates to a novel process for the immobilization of the enzyme on biocompatible gold nanoparticle encapsulated free-standing membranes. The binding of the enzyme occurs through the amine groups and cysteine residues to the gold nanoparticles. The free-standing gold nanoparticle membrane was synthesized at the interface between chloroform...

20060141597 - Valency platform molecules comprising aminooxy groups: Molecules comprising aminooxy groups are provided, wherein the aminooxy groups provide attachment sites for the covalent attachment of other molecules. In one embodiment, polyoxyethylene molecules comprising aminooxy groups are provided that can be conjugated to wide variety of biologically active molecules including poly(amino acids). In another embodiment, valency platform molecules...

20060141598 - Promotion of peroxisomal catalase function in cells: The molecular mechanisms of peroxisome biogenesis have begun to emerge: in contrast, relatively little is known about how the organelle functions as cells age. The present inventors characterized age-related changes in peroxisomes of human cells and showed that aging compromises peroxisomal targeting signal 1 (PTS1) protein import, with the critical...

20060141599 - Crystals of glucokinase and methods of growing them: Crystalline forms of mammalian Gluckokinase of sufficient size and quality to obtain structural data by X-ray crystallography are presented. Methods of growing such crystals are also disclosed....

20060141600 - Methods and compositions related to argonaute proteins: This invention provides methods and compositions related to Argonaute proteins and, in certain embodiments, the applications of these methods and compositions to treatment and therapeutics based on RNAi....

20060141601 - Egvii endoglucanase and nucleic acids encoding the same: The present invention provides a novel endoglucanase nucleic acid sequence, designated egl7, and the corresponding EGVII amino acid sequence. The invention also provides expression vectors and host cells comprising a nucleic acid sequence encoding EGVII, recombinant EGVII proteins and methods for producing the same....

20060141602 - Packaging of positive-strand rna virus replicon particles: The invention generally relates to recombinant polynucleotides, positive-strand RNA virus (psRNAV) recombinant expression vectors, and packaging systems. The packaging systems are based on the expression of helper functions by coinfecting re-combinant poxvirus vectors comprising recombinant polynucleotides. Methods for obtaining psRNAV replicon particles using these packaging systems are disclosed. Immunogenic compositions...

20060141603 - Methods and compositions for selectively enriching microbes: The present invention provides methods and materials to selectively enrich target microbes in a sample....

20060141605 - Biological neutralization of highly alkaline textile industrial wastewater: The present invention provides a novel process of neutralizing textile industrial wastewater by a bacterial strain isolated in India. The isolated bacterial strain is capable to bring down the pH of wastewater from 12.00 to 7.00 units within two hours. The neutralization of alkaline textile industrial wastewater by such biotechnological...

20060141604 - Biotechnological process for neutralizing alkaline beverage industrial waste water: The present invention provides a process of neutralizing beverage industrial wastewater by a bacterial strain Exiguobacterium sp. isolated in India, which strain is capable of bring down the pH of wastewater from 12.00 to 7.00 units within 1 to 1.5 hours....

20060141606 - Enantioconvergent chemoenzymatic synthesis of (r)-gamma-amino-beta-hydroxybutyric acid ((r)-gabob): The present invention particularly relates to a chemoenzymatic process for the stereoselective preparation of both enantiomers of 3-hydroxy-4-trityloxy butanenitrile key intermediates for the preparation of (R)-GABOB by lipase mediated kinetic resolution of its racemates and their effective application in the enantioconvergent synthesis of (R)-GABOB....

20060141607 - Capillary perfused bioreactors with multiple chambers: A bioreactor for cultivating living cells in a liquid medium. In one embodiment of the present invention, the bioreactor includes a first substrate having a first surface, an opposite second surface and edges. The bioreactor further includes a second substrate having a first surface and an opposite second surface, defining...

20060141609 - Culture observation apparatus, sample tray heat-insulating device and lid: A culture observation apparatus, which is used for observing a cultured cell while culturing the cell, includes a culture device that forms a culture space which is controlled to be an environment suitable for culturing the cultured cell; a sample tray that holds a container housing the cultured cell and...

20060141608 - Piezoelectric sensor arrangement: A piezoelectric sensor arrangement for analysis of fluid samples includes a signal source, a measuring device and a docking system. The sensor arrangement comprises a first part provided with means for receiving a sensor element that exposes a piezoelectric quartz crystal, a second part comprising fluid channels for the sample...

20060141612 - Automated method of manufacturing polymer arrays: The present invention provides methods to process multiple sensors by providing a sensor plate and HT plates. In a preferred embodiment of the invention, methods for assembling microarray pegs and microarray plates are described for high throughput microarray processing....

20060141610 - Microarray devices having controllable reaction volume: This invention relates generally to the field of microarray reaction devices and uses thereof. In particular, the invention a microarray reaction device wherein a plurality of reaction spaces are formed between a first and second plurality of projections, the heights of said plurality of reaction spaces being substantially identical and...

20060141611 - Spatially scanned optical reader system and method for using same: An optical reader system is described herein that uses a scanned optical beam to interrogate a biosensor to determine if a biomolecular binding event occurred on a surface of the biosensor. In one embodiment, the optical reader system includes a light source, a detector and a processor (e.g., computer, DSP)....

20060141613 - Culture observation apparatus: A culture observation apparatus, which is used for observing a cultured cell while culturing the cell, includes a culture device that cultures the cultured cell, and a microscope used for observing the cultured cell. The culture device includes a first space that is controlled to an environment suitable for culture...

20060141614 - Device and method for parallel, automated cultivation of cells in technical conditions: The invention relates to a device for the cultivation of cells in liquid columns measured on a millimetre scale. Said device comprises a container for receiving a liquid culture suspension of the cells, a mixing element for mixing the culture suspension in the container around a mixing axis. The container...

20060141615 - Vegetable alga and microbe photosynthetic reaction system and method for the same: A vegetable alga and microbe photosynthetic reaction system and method for the same is used for vegetable algae and microbe cultivation liquid and algae seeds infused herein to circularly conduct photosynthesis and discharge oxygen; the vegetable alga and microbe photosynthetic reaction system comprises a photosynthetic reaction unit, a pressure liquid...

20060141616 - Cell colonies dissecting and transplanting apparatus: A cell colonies dissecting and transplanting apparatus comprises: a base, a platform, a feeding mechanism having at least three axis feeding means, a movable holder, a cutting means, a transplanting means, and a CCD image capturing means. The platform having the CCD image capturing means therebottom is disposed onto the...

20060141619 - Apparatus and method for machining microchamber for cell culture: There is provided a processing apparatus and a processing method for micro-chamber for cell culture having a desired culturing space. The micro-chamber processing apparatus for cell culture of the present invention comprises a micro-chamber comprising no more than one absorption layer and at least one gel layer, in this order,...

20060141618 - Cell separation apparatus: A cell separation apparatus comprises a means for moving a cell within a cell separating space by applying an electrical voltage to cells in the cell separating space using gel electrodes, and channels for separating and discharging the cell, which thus does not damage a cell sample and prevents the...

20060141617 - Multilayered microcultures: A multilayer microculture capable of modeling complex in vitro structures such as mammalian tissues and organ structures is provided, along with methods for producing such a microculture and methods of using such microcultures for assaying for modulators of cell-cell interaction, cell migration, cell proliferation, cell adhesion or cellular or organismal...

20060141620 - System and method for forming a cardiac tissue construct: A system and method for forming a cardiac tissue construct includes a chamber and cardiac myocytes provided within the chamber. The chamber is arranged to at least partially surround an intact blood vessel in vivo to facilitate formation of the three-dimensional cardiac tissue construct within the chamber....

20060141621 - Process for producing tissue cell from pluripotent stem cell derived from iris pigment epithelial cell of animal and tissue cell obtained by the process: A method for producing tissue cells derived from iris pigmented epithelial cells of an animal, and tissue cells obtained by the method are provided. The method and the tissue cells solve problems such as immunological rejection in cell transplantation, ethical issues, and unbalance between the demand and supply of transplant...

20060141622 - Transplantation of human neural cells for treatment of neurodegenerative conditions: A method of treating neurodegenerative conditions is provided. Neural stem cells may be implanted at and/or remote from a region of neuron degeneration. The methods can include isolating neural stem cells from regions where specific types of neurons corresponding to the neurons to be replaced are generated. The methods can...

20060141623 - Automated tissue engineering system: The invention provides systems, modules, bioreactor and methods for the automated culture, proliferation, differentiation, production and maintenance of tissue engineered products. In one aspect is an automated tissue engineering system comprising a housing, at least one bioreactor supported by the housing, the bioreactor facilitating physiological cellular functions and/or the generation...

20060141624 - Method for transferring molecules in vital cells by means of laser beams and arrangement for carrying out said method: The invention relates to an optical method for targeted transfer of molecules, preferably of DNA, RNA, peptides, amino acids and proteins, into vital cells by means of laser radiation and to an arrangement for implementing the method. The object of the invention, to find a novel possibility for targeted molecule...

20060141625 - Expression cassette and vector for transient or stable expression of exogenous molecules: The disclosure provides an expression cassette and a vector comprising the cassette for expression of a polynucleotide. The expression cassette includes a promoter/enhancer, an intervening region, and a polyadenylation signal domain. Expression systems and methods of using the expression cassette and vector are also provided....

20060141626 - Non-random method of gene shuffling: The present invention concerns the non-random assembling of DNA molecules in a DNA construct and methods of using such constructs, including the production of nucleic acid libraries. The non-random gene shuffling is preferably accomplished by the following steps. First, optionally, the amino acid sequences of proteins encoded by related gene...

20060141627 - Vectors for stable gene expression: The present invention relates to expression vectors capable of promoting transgene expression. The expression vectors include site specific recombination elements, insulator elements, and recombinase elements. In particular, the present invention provides methods for obtaining specific and stable integration of nucleic acids into eukaryotic cells through site specific recombination....

20060134597 - Apparatus and method for rapid separation of cells without using density gradient and antibodies: A method and an apparatus for rapid cell separation without using density gradient and antibody but using a column packed with resin particles. The interactions between cell surfaces and resin particles resulting in the different retention time of different cells in the column contribute to the separation of the specific...

20060134596 - Cryopreservation of human blastocyst-derived stem cells by use of a closed straw vitrification method: An improved method for vitrification of biological cells, especially blastocyst-derived stem cells (BS cells). The method is very mild for the cells that remain viable after they have been thawed. The method comprises, i) transfer of the cells to a first solution (solution A), ii) optionally incubation of the cells...

20060134595 - Polymer affinity matrix, a method for the production and use thereof: A polymer affinity matrix for the binding of one or more substances in a fluid for removing said substance(s) from the fluid and/or decreasing the amount or concentration thereof in said fluid with a view to preventing, eliminating, or reducing undesired activation of components in said fluid is described, as...

20060134598 - Cell culture media dispenser: An apparatus and method of dispensing precise aliquots of cell culture media from a bulk, sterile, flexible container. The apparatus has load cell, a control valve, and a programmable controller. The container suspends from the load cell within the housing. The load cell continuously transmits to the controller an output...

20060134603 - Automated extraction and purification of samples using optical tweezers: An apparatus and method for purifying a forensic sample using an automated extraction and purification system includes optical tweezers; an input channel through which the sample is introduced; a chamber which receives the sample from the input channel; a collection channel through which selected particles of the sample are removed;...

20060134604 - Flexible method and apparatus for high throughput production and purification of multiple proteins: A plurality of proteins of interest, or peptides of interest, or other genetically expressed materials, are screened and subsequently produced using any of a variety of expression systems. The plurality of proteins are extracted from a plurality of separate, processed green juices, each green juice containing one of the proteins...

20060134600 - Method and devices for non-traumatic movement of a probe through biological cell material: A method for moving a probe (10, 91) through a cell material (20), which is formed from biological cells (21), is described, the probe (10) displacing the cells (21) without injury. A probe (10, 91) for performing the method and a cell manipulator, which is equipped with at least one...

20060134602 - Methods of identifying cytotoxic effects in quiescent cells: The present invention provides a system for measuring the cytotoxicity of a test compound against quiescent cells. The inventive methods and reagents may also be used to determine whether a compound, such as a chemotherapeutic agent, will have undesired toxicity toward normal tissues, which generally do not include a significant...

20060134599 - Microfluidic device for cell separation and uses thereof: The invention features methods for separating cells from a sample (e.g., separating fetal red blood cells from maternal method begins with the introduction of a sample including cells into one or more microfluidic channels. In one embodiment, the device includes at least two processing steps. For example, a mixture of...

20060134601 - Osmotic reaction detector for detecting biological and non-biological reactions: A method and apparatus for measuring the presence or absence of interaction between at least a first and second material of interest by measuring osmotic pressure changes in an osmotic cell. Changes in osmotic pressure are determined by measuring the amount of compression exhibited by a compressible, semi-permeable material positioned...

20060134605 - Platelet biomarkers for the detection of disease: The present inventors have surprisingly discovered that platelets sequester angiogenic regulators and prevent their degradation. Thus, by analyzing levels of angiogenic regulators in platelets, it is now possible to detect angiogenic activity, even at an early stage. By monitoring for changes in angiogenic activity, the presence of cancer or other...

20060134608 - Chemically amplified electrochemical detection of affinity reaction: This invention relates generally to the field of electrochemistry. In particular, the invention provides a method for assaying an analyte, using, inter alia, a reactant capable of binding and/or reacting with an analyte to be analyzed on an oxide electrode and a reducing agent not capable of being oxidized directly...

20060134610 - Chris-like virus, compositions, vaccines and uses thereof: A novel virus, the Chris-like virus, has been identified and characterised. Sequences derived from a new paramyxovirus, Chris-like virus (CV), are provided. These sequences encode viral proteins with similarity to recognized viral proteins in other known and characterized paramyxoviruses. The CV viral sequences and the polypeptides encoded therein are useful...

20060134609 - Compositions and methods for determining the presence of sars coronavirus in a sample: The present invention relates to oligonucleotides useful for determining the presence of SARS coronavirus in a test sample. The oligonucleotides of the present invention may be incorporated into detection probes, capture probes and amplification oligonucleotides, or used in various combinations thereof....

20060134611 - Condom device for detecting std's: A condom device for detecting STD's comprising an outer layer having open pores positioned along the surface of the outer layer. The condom device includes an inner layer positioned opposite the outer layer. An intermediate layer is positioned between the outer layer and the inner layer. The intermediate layer receives...

20060134613 - Detection of microbe contamination on elastomeric articles: An elastomeric article that contains a chromogen that undergoes a detectable change in color in the presence of one or more microbes is provided. For example, in one embodiment, the chromogen is a solvatochromic dye (e.g., Reichardt's dye) that undergoes a color change in the presence of bacteria or other...

20060134607 - Marker gene: A method of determining a predisposition to infection, especially infection with HIV, together with therapy for the infection is described....

20060134615 - Method for the simultaneous detection of hpv rna and dna in a sample: A method is provided for the simultaneous detection of human papillomavirus DNA and RNA in a sample....

20060134612 - Methods and compositions for retroviral preintegration complex-based assays: Disclosed are solution-based methods of screening for molecules that modulate the integration of retroviral cDNA into target DNA molecules. These methods can be used to identify molecules that modulate (e.g., inhibit) the integration of unintegrated retroviral cDNA. Such PIC activity modulators may have therapeutic application, for example, to treat or...

20060134614 - Screens for altered immune response capability: The invention relates to associations between genetic variation in the gene encoding CD45 and human disease and human immune responses. In particular the invention provides methods of screening human subjects for the presence of an “altered immune response capability”, which may in turn affect susceptibility to viral disease and/or autoimmune...

20060134616 - Sonication to selectively lyse different cell types: A sonication apparatus is directed to a microfluidic-based system to automate differential extraction of specific cell types within a mixed sample. The microfluidic-based system includes a sonication module for selective cell lysis, separating means to eliminate centrifugation, high surface area pillar chip modules to purify DNA from a cell lysate,...

20060134606 - Substrates for isolating reacting and microscopically analiyzing materials: An immobilizing device for biological material comprises a rigid support (12) carrying a substrate layer (20, 20′) of polymer having biological immobilizing properties, e.g. for amino and nucleic acids. Substantially solid ultra-thin substrate layers (20′) having a thickness less than about 5 micron, preferably between about 0.1 and 0.5 micron,...

20060134654 -