Chemistry: molecular biology and microbiology inventions 06/06

20060141444 - Adherent entities and uses therefor: The present invention is directed to reproductive function-modulating organisms especially of the genera Ureaplasma and Mycoplasma, which adhere more strongly to spermatozoa than other organisms and which correlate with the presence or risk of a condition selected from male infertility, an adverse pregnancy outcome or an adverse assisted reproductive technology...

20060141443 - Apparatus and method for transcellular testing: The present invention relates to a device and method for facilitating high throughput transcellular flux testing of compounds, such as pharmaceuticals or drugs, other compounds, or compound combinations. In one embodiment, the system and methods of the present invention may be used to identify the optimal components (e.g., solvents, carriers,...

20060141447 - Cell culture media for mammalian cells: A chemically defined mammalian cell culture medium is provided that supports maintenance and long term clonal growth of mammalian hepatocytes and other cells....

20060141445 - Method for manufacturing a sensor chip and sensor chip blank: In a process for manufacturing a sensor chip a support is prepared having at least one surface region in which at least one reactive group is arranged. A buffer solution is applied to the surface region for coating at least one surface region. Then, at least one receptor having binding...

20060141446 - Substrates, devices, and methods for cellular assays: The present invention relates to the field of molecular diagnostics, and in particular to diagnostics based on a liquid crystal assay format. In particular, the present invention provided improved substrates and methods of using liquid crystal assays for quantitating the amount of an analyte in a sample. The present invention...

20060141448 - Whole cell assay: This invention relates to newly developed methods for discovering therapeutic compounds using a cell-based assay system. This invention also relates to compositions of matter useful in carrying out the methods of the invention as well as therapeutic compounds developed using such methods....

20060141449 - Yeast screens for treatment of human disease: Screening methods for identifying substances that provide therapeutic value for various diseases associated with protein misfolding are provided. Genetic and chemical screening methods are provided using a yeast system. The methods of the invention provide a rapid and cost-effective method to screen for compounds that prevent protein misfolding and/or protein...

20060141450 - Magnetism based rapid cell separation: This invention relates generally to the field of. In particular, the invention provides processes and kits for isolating a target cell, cellular organelle or virus from a sample, using inter alia, non- or low-specific binding between a target cell, cellular organelle or virus with a magnetic microbead....

20060141457 - Acyltransferase: The invention relates to at least one nucleotide sequence, derived from a nucleotide sequence encoding an acyltransferase polypeptide comprising at least one membrane-spanning region, encoding an improved active membrane independent acyltransferase polypeptide in which at least one amino acid residue of the membrane-spanning region has been deleted and/or substituted as...

20060141521 - Apparatus, methods and compositions for biotechnical separations: RNA, preferably messenger RNA, is purified by use of selective precipitation, preferably by addition of compaction agents. Also included is a scaleable method for the liquid-phase separation of DNA from RNA. RNA may also be recovered by fractional precipitation according to the invention. We have discovered that specific classes of...

20060141471 - Assay for detection of renal cell carcinoma: Methods for diagnosing and monitoring renal cell carcinoma in a subject comprising detecting kallikrein polypeptides, or polynucleotides encoding the polypeptides in a sample from the subject. The kallikrein polypeptides include kallikrein 5, kallirekin 6, kallikrein 10, and kallikrein 11....

20060141460 - Assays for fprl-1 ligands: Assays for screening for compounds that act as agonists, antagonists, or inverse agonists of CKβ8-1 at the FPRL-1 receptor....

20060141493 - Atherosclerotic phenotype determinative genes and methods for using the same: Genes whose expression is correlated with and determinant of an atherosclerotic phenotype are provided. Genes whose expression is correlated with and determinant of an atherosclerotic susceptibility are also provided. Also provided are methods of using the subject atherosclerotic determinant genes or the atherosclerotic susceptibility genes in diagnosis and treatment methods,...

20060141474 - Biomolecule detecting element and method for analyzing nucleic acid using the same: An inexpensive DNA chip/DNA microarray system capable of highly accurate measurement with low running cost. A DNA probe 8 is immobilized on a floating electrode 7 connected to a gate electrode 5 of a field effect transistor. Hybridization with a target gene is carried out on the surface of the...

20060141508 - Cellular signaling pathway based assays, reagents and kits: The present invention is directed to an assay system, PAM (Pathway Assay Matrix) for screening drug to determine whether they are effective for treating or preventing a disease or disorder. This assay system takes into account that many diseases involve complex pathways, and measures the effect of a particular drug...

20060141511 - Chemical amplification for the synthesis of patterned arrays: Radiation-activated catalysts (RACs), autocatalytic reactions, and protective groups are employed to achieve a highly sensitive, high resolution, radiation directed combinatorial synthesis of pattern arrays of diverse polymers. When irradiated, RACs produce catalysts that can react with enhancers, such as those involved in autocatalytic reactions. The autocatalytic reactions produce at least...

20060141505 - Chimeric fusion molecule for analyte detection and quantitation: Detector fusion molecules are produced by attaching a protein sub-unit to a linker, and attaching the linker to a nucleic acid molecule. The detector fusion molecules have utility in detecting and quantifying a specific target analyte from a sample. The protein sub-unit of the detector fusion molecule is selected to...

20060141470 - Chlamydia pneumoniae associated chronic intraocular disorders and treatment thereof: Infection by Chlamydia pneumoniae has been identified as a risk factor associated with the pathogenesis of certain chronic intraocular disorders, for example, age-related macular degeneration. The invention provides methods of identifying individuals at risk of developing chronic intraocular disorders, and methods of delaying and/or preventing the onset of such chronic...

20060141479 - Cngh0010 specific polynucleotides, polypeptides, antibodies, compositions, methods and uses: Novel polypeptides (CNGH0010) and antibodies, including specified portions or variants, specific for at least one such CNGH0010 polypeptide, variant, or fragment thereof, as well as nucleic acids encoding such CNGH0010 polypeptides and antibodies, complementary nucleic acids, vectors, host cells, and methods of making and using thereof, are useful for therapeutic...

20060141519 - Compositions and methods for diagnosing autism: Mutations located within the gene encoding the homeobox transcription factor, ENGRAILED 2 (EN2), have now been identified as molecular markers associated with susceptibility for autism and related disorders. Thus, the present invention relates to compositions in the form of diagnostic kits, primers and target sequences, for use in methods for...

20060141491 - Compositions and methods for enhancing polynucleotide amplification reactions: Compositions and methods for enhancing PCR and other polynucleotide replication reactions are disclosed. These comprise the addition of low molecular weight organic amides, sulfones or sulfoxides to PCR or other replication reaction mixtures....

20060141516 - De-novo sequencing of nucleic acids: The present invention is directed to an improved analysis procedure for the comparative sequencing of nucleic acids using multistage mass spectrometry. More precisely, the invention is directed to a method enabling the de-novo sequencing of nucleic acid molecules using multistage mass spectrometry....

20060141518 - Detection of gene expression: Methods for detection of nucleic acids such as a cDNA copy of an mRNA are disclosed. The methods comprise using a PCR to form a preamplification product which comprises cDNA sequence as well as primer target sequences and a detection probe sequence, which are introduced by the forward and reverse...

20060141503 - Detection of sequence variation of nucleic acid by shifted termination analysis: The invention relates to a method for detecting any mutation at a predetermined site occurring in a known nucleic acid sequence. The method uses primer extension analysis to detect the mutation. Unlabeled terminator is supplied along with labeled non-terminator in the primer extension reaction to detect whether the first nucleic...

20060141459 - Diagnostic and therapeutic use of foap-13 polynucleotides and polypeptides for neurodegenerative diseases: The present invention discloses the dysregulation of foap-13 gene expression in specific brain regions of Alzheimer's disease patients. Based on this finding, the invention provides a method for diagnosing or prognosticating Alzheimer's disease in a subject, or for determining whether a subject is at increased risk of developing Alzheimer's disease....

20060141485 - Electrically active combinatorial chemical (eacc) chip for biochemical analyte detection: Apparatus and methods are disclosed for electrically active combinatorial-chemical (EACC) chips for biochemical analyte detection. An apparatus includes a substrate that has an array of regions defining multiple cells, wherein each of the cells includes a reaction cavity that contains multiple functional binding groups. A method of detecting an analyte...

20060141473 - Erk7 and erk8, novel diagnostic markers for cancer: The present invention is directed in part to the discovery of a novel signal transduction pathway that regulates estrogen responsiveness. Human extracellular signal-regulated kinase 8 (ERK8) has been discovered by applicants to preferentially enhance the destruction of ERα, and loss of ERK8 is correlated with breast cancer progression. Thus monitoring...

20060141492 - Gene probes for the selective detection of microorganisms that reductively dechlorinate polychlorinated biphenyl compounds: The present invention relates to an assay for identification of PCB dechlorinating organisms that are capable of biologically removing PCBs from contaminated materials. Specifically, the invention provides a set of primers for detecting PCB dechlorinating organisms in a sample. These individual primers of the primer set have a sequence of...

20060141455 - Gene products differentially expressed in cancerous breast cells and their methods of use: The present invention provides polynucleotides, as well as polypeptides encoded thereby, that are differentially expressed in breast cancer cells. These polynucleotides are useful in a variety of diagnostic and therapeutic methods. The present invention further provides methods of reducing growth of breast cancer cells. These methods are useful for treating...

20060141476 - Growth arrest homeobox gene: A novel growth arrest homeobox gene has been discovered and the nucleotide sequences have been determined in both the rat and the human. The expression of the novel homeobox gene inhibits vascular smooth muscle cell growth. The growth arrest homeobox gene hereinafter referred to as the “Gax gene” and its...

20060141451 - Guinea pig proteinase-activated receptor 4 and its activating peptide: This invention relates to nucleic acid encoding guinea pig PAR 4 and to the protein encoded thereby. The guinea pig DNA and protein are useful for the development of models of human platelet aggregation. The invention further relates to an animal model to assess the role of PAR antagonists in...

20060141522 - Heregulin-like factor: The present invention relates to a novel HLF protein which is a member of the heregulin family. In particular, isolated nucleic acid molecules are provided encoding the human HLF protein. HLF polypeptides are also provided as are vectors, host cells and recombinant methods for producing the same. The invention further...

20060141481 - Hsv-1 and hsv-2 primers and probes: The present invention provides methods, primers and probes for the detection of HSV nucleic acids in biological fluids and tissue. In the methods of the invention, at least a portion of HSV nucleic acid present in a biological sample suspected of containing an HSV-1 and/or HSV-2 is amplified and the...

20060141462 - Human type ii diabetes gene-slit-3 located on chromosome 5q35: Association of Type II diabetes and a locus on chromosome 5q35 is disclosed. In particular, the gene SLIT-3 with this locus is shown by linkage analysis to be a susceptibility gene for Type II diabetes. Pathway targeting for drug delivery and diagnosis applications in identifying those have Type II diabetes...

20060141523 - Integrated method for pcr cleanup and oligonucleotide removal: A method is provided for purifying a desired polynucleotide product by removing unincorporated oligonucleotides from a polymerase or ligase reaction mixture....

20060141501 - Iterative probe design and detailed expression profiling with flexible in-situ synthesis arrays: Methods and compositions are provided that are useful for detecting and reporting a plurality of different target polynucleotide sequences in a sample, such as polynucleotides corresponding to a plurality of different genes expressed by a cell or cells. In particular, the invention provides methods for screening a plurality of candidate...

20060141494 - Luminescence detection apparatus: An object of the present invention is to provide a luminescence detection apparatus compact in size which is capable of conveniently determining DNA base sequences at a low cost. According to the present invention, a luminescence detection apparatus 1 is provided comprising: a plurality of reaction cells 6 each having...

20060141507 - Manufacture and use of non-standard size microarray slides: Methods and devices are disclosed for microarray analysis. In one embodiment a method is disclosed for processing a non-standard size slide having an array of chemical compounds attached to a surface of the slide. A sample is exposed to the surface of the non-standard size slide wherein components in the...

20060141472 - Medical use of ras antagonists for the treatment of capillary malformation: The invention relates to the field of vascular anomalies and methods for diagnosing and treating them. The invention provides for the causative gene (RASA1) and mutations therein which are useful for diagnosis of inherited capillary malformations. The invention further provides RASA1 antagonists for use in treatment of capillary malformations....

20060141482 - Method and multicomponent conjugates for treating cancer: The present invention provides a method for treating cancer in a mammal and to multicomponent conjugates useful in such treatment. In particular, it provides a cancer treatment method that includes administering multicomponent conjugates of formula: A-B-C, where “B” may be present or absent, “A” comprises at least one specific anti-cancer...

20060141466 - Method and sensor for detecting a chemical substance using an optically anisotropic material: A method and sensor for detecting a chemical substance in an analyte. An anisotropic material is subjected to the analyte. Light is passed through the anisotropic material and collected. A change of an optical anisotropy of the collected light is detected, the change being indicative of the chemical substance in...

20060141464 - Method for immobilizing biologic molecules on solid surfaces: The invention provides a method for immobilization of biological molecules such as nucleic acids, peptides and proteins onto the surface of a glass or plastic solid support....

20060141510 - Method for information processing with nucleic acid molecules: The present invention is directed to provide an information processing method using autonomously workable nucleic acids, and a molecular computer to carry out operations with the method. Objectives above mentioned may be achieved with following method. The present invention provides an information processing method carrying out operations with functions receiving...

20060141513 - Method for recognition and recovery of cis-regulatory modules: A computational method is described which uses the process of cis-regulatory module evolution to identify conserved sequence patches which exhibit suppression of change by snp/indel occurrence, in the absence of having to execute multiple interspecific sequence comparison analysis, including libraries, and arrays that contain such cis-regulatory modules....

20060141488 - Method for stabilizing or preserving nucleic acid by using amino surfactants: A method for stabilizing or persevering nucleic acids by forming an insoluble ionic complex between nucleic acids and a surfactant in a biological sample, consisting of a step of contacting the biological sample with an isolation reagent comprising amino surfactants of the formula (I): R1R2R3N(O)x, (I), wherein, R1 and R2...

20060141452 - Method for synthesizing single-stranded nucleic acid: This invention relates to a method for selectively and efficiently synthesizing one of the sense or antisense strands of double-stranded nucleic acid. The method for synthesizing single-stranded nucleic acid according to this invention comprises the following steps of: 1) cleaving, with a restriction enzyme, double-stranded DNA having a restriction enzyme...

20060141463 - Method of detecting bone paget's disease: A method for detecting Paget disease of bone and an animal showing the pathology of Paget disease of bone are provided. In particular, there are provided associating Paget disease of bone with the mutation a chondroitin/chondroitin synthase gene comprising the amino acid sequence depicted in any one of SEQ ID...

20060141458 - Method of identifying genes involved in cell growth regulation: A method is provided for identifying in eukaryotes nucleic acid sequences that are involved in or suspected of being involved in cell growth regulation comprising mutagenising wild type plants or plants genetically modified to have accelerated tissue growth and selecting mutants having relative to the background and altered phenotype, followed...

20060141465 - Method of immobilizing a polynucleotide on a solid support, solid immobilization support thus obtained, method of detecting target polynucleotide from the solid support and solution for immobilizing the polynucleotide: It is intended to provide a method of immobilizing a polynucleotide on a solid support by spotting the polynucleotide on the solid support in a uniform spot shape without any irregularity. Namely, a method of immobilizing a polynucleotide on a solid support by spotting a solution containing the polynucleotide together...

20060141496 - Method of screening substance interacting with abc protein: The present invention relates to a method for measuring transporter activity of ABC protein that may measure not only small amount of samples with small data fluctuation and good reproducibility, but also many samples simultaneously and concisely; a screening method for substances that interact with ABC protein using said measuring...

20060141489 - Method of statistical genomic analysis: A method of design and analysis of microarray studies has been developed. The method includes classification of the microarrays; measurement of the reliability and validity of expression measurements; estimation of the effect of an experimental manipulation on gene expression; design of a statistical analysis to determine the distinction between biological...

20060141478 - Methods and compositions for detecting and treating genetically induced chronic diseases: The present invention discloses a functional relationship between a recognized disease condition and a polymorphism in the nucleotide factor kappa B promoter (NFKB1). This relationship provides a platform for methods of altering promoter activity and for determining similar relationships between specific pathologies and identified polymorphisms. A statistically significant risk of...

20060141456 - Methods and compositions for milieu-dependent binding of a targeted agent to a target: The present invention provides methods and compositions for milieu-dependent binding of a targeted agent to a target, for example, for the milieu-dependent binding of a diagnostic or therapeutic molecule to a diseased, injured or infected organ, tissue or cell....

20060141502 - Methods and compositions for the preparation and use of fixed-treated cell-lines and tissue in fluorescence in situ hybridization: This invention relates to methods for the detection of one or more mRNA transcripts in paraffin-embedded tissue by “mRNA liberation in fixed-treated tissue or ‘MLIFTT’”. This method includes treating the tissue with ammonia-ethanol and sodium borohydride combined with pressure cooking of the tissue. The chemical treatments reduce the tissue autofluorescence...

20060141500 - Methods and compounds for the diagnosis of inflammatory disease and identification of pharmacological agents useful in the treatment of inflammatory disease: Methods for the diagnosis of inflammatory bowel diseases and the identification of agents useful in the treatment of such diseases based upon the agent's effect on reducing Pim-2 expression....

20060141467 - Methods and kits for making double stranded ribonucleic acids: Embodiments of the present invention are directed to methods and kits for the generation of double stranded RNA by RNA dependent RNA polymerases. The methods and kits feature exponential generation of such RNAs through simple steps/ The RNA is suitable for interference with expression in cells....

20060141498 - Methods for fragmenting nucleic acid: Methods for using an apurinic/apyrimidinic endonuclease, capable of cleaving both single- and double-stranded cDNA, for fragmentation and labeling of single stranded or double stranded DNA molecules are provided. Amplification methods that generate single-stranded amplified cDNA are also disclosed. In the subject methods AP sites in a population of nucleic acids...

20060141506 - Methods for monitoring the expression of alternatively spliced genes: Methods, probe arrays and computer software products are provided for determining the arrangement of sequence elements. In one embodiment, methods for making and using exon chips are provided. The exon chips may be used to identify and quantify splice variants....

20060141520 - Methods for the treatment of metabolic disorders, including obesity and diabetes: The present invention relates to methods and compositions for the diagnosis and treatment of metabolic disorders, including, but not limited to, obesity, diabetes, overweight, anorexia, or cachexia. The invention further provides methods for identifying a compound capable of treating a metabolic disorder. The invention also provides methods for identifying a...

20060141461 - Methods of assessing the risk of reproductive failure by measuring telomere length: The invention features a method of identifying oocytes with a risk of reproductive failure and/or aneuploidy based on a telomere length assay....

20060141499 - Methods of determining human egg competency: The inventors have discovered inter alia, that when the chromosome complement of a first polar body (PB-I) is normal, it alone is in more than 80% of cases sufficient to predict that an embryo which has developed from an oocyte associated with the PB-I is also normal and competent. As...

20060141487 - Methods, compositions, and kits for amplifying and sequencing polynucleotides: In one aspect, there are provided methods of amplifying and sequencing a polynucleotide. In some embodiments, the method includes (a) amplifying the polynucleotide with at least one amplification primer, a processive amplification polymerase, a sequencing primer, a sequencing polymerase, deoxynucleoside triphosphates suitable for template-dependent primer extension, and one or more...

20060141497 - Molecular analysis of cellular fluid and liquid cytology specimens for clinical diagnosis, characterization, and integration with microscopic pathology evaluation: The application relates to methods, materials, kits, and devices for characterizing fluid specimens of any type or source that may or may not contain cells. The methods, materials, kits, and devices can be used for analyzing fluid obtained from various organs or near a purported tumor site, such as via...

20060141504 - Molecular technologies for improved risk classification and therapy for acute lymphoblastic leukemia in children and adults: The present invention relates to methods for predicting the outcome of therapeutic intervention in cases of leukemia, especially acute lymphoblastic leukemia in children and adults. The present invention evaluates a gene expression profile and identifies prognostic genes of cancers, in particular leukemia, more particularly B-precursor acute lymphoblastic leukemia (ALL). The...

20060141469 - Multi-layered electrochemical microfluidic sensor comprising reagent on porous layer: The present invention relates to a microfluidic electrochemical sensor apparatus and a method for conducting analytical tests with said apparatus for multi-reactant assays. The apparatus of this invention is a multi-layer body made of at least three layers, the first one being a polymer layer (1) comprising a microstructure (5)...

20060141490 - Myd88 as a therapeutic target for cancer: The invention relates to methods for identifying anti-cancer agents using MyD88 as a therapeutic target....

20060141524 - Novel kinases and uses thereof: Novel kinase polypeptides, proteins, and nucleic acid molecules are disclosed. In addition to isolated, full-length kinase proteins, the invention further provides isolated kinase fusion proteins, antigenic peptides, and anti-kinase antibodies. The invention also provides kinase nucleic acid molecules, recombinant expression vectors containing a nucleic acid molecule of the invention, host...

20060141512 - Novel method for separation of human sperm from biological samples for application in human identification: The development of an isolation methodology for separation of human sperm cells from biological samples containing human epithelial cells is provided. Using sperm binding proteins, glycopeptides, lectins, derivatives of N-acetylglucosamine, triazine dyes or inhibitors of glycosyltransferase linked to an insoluble support, our invention enables binding of human sperm cells from...

20060141468 - Novel notch-like polypeptides: The present invention discloses open reading frames (ORFs) in human genome encoding for novel notch-like polypeptides, and reagents related thereto including variants, mutants and fragments of said polypeptides, as well as ligands and antagonists directed against them. The invention provides methods for identifying and making these molecules, for preparing pharmaceutical...

20060141495 - Polymorphic markers and methods of genotyping corn: Polymorphic corn DNA loci useful for genotyping between at least two varieties of corn. Sequences of the loci are useful for designing primers and probe oligonucleotides for detecting polymorphisms in corn DNA. Polymorphisms are useful for genotyping applications in corn. The polymorphic markers are useful to establish marker/trait associations, e.g....

20060141475 - Polynucleotide sequence detection assays and analysis: Methods and software for associating mobility probes with target macromolecules are discussed. By encoding the identities of macromolecules of interest with a universal set of tag portions complementary to a universal set of mobility probes, reactions varying in their input starting material may be identified using the same universal set...

20060141515 - Polynucleotides encoding plant cysteine proteases: This invention relates to an isolated nucleic acid fragment encoding a proteinase. The invention also relates to the construction of a chimeric gene encoding all or a portion of the proteinase, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the...

20060141486 - Porous substrates and arrays comprising the same: The present invention relates to arrays comprising porous substrates for attachment of nucleic acids, polypeptides, membranes, or other biological or organic materials. In many embodiments, the arrays of the present invention have a flow-through configuration such that washing buffers or samples can access to the porous substrates from at least...

20060141453 - Prokineticin polypeptides, related compositions and methods: The invention provides isolated polypeptides that stimulate gastroeintestinal smooth muscle contraction, including human prokineticin 1 and human prokineticin 2 polypeptides, and functional fragments and modifications thereof. Also provided are methods of stimulating gastrointestinal smooth muscle contraction in a mammal, by administering to the mammal an effective amount of a prokineticin...

20060141517 - Protein having pdz domain sequence: While analyzing changes in gene expression by TNFα in human umbilical vascular endothelial cells, a gene showing enhanced expression due to stimulation with TNFα was isolated. After screening with this gene as a probe, a gene encoding a protein was isolated. Analysis of the protein encoded by this isolated gene...

20060141484 - Reactive ion etched substrates and methods of making and using: Disclosed herein are substrates comprising reactive ion etched surfaces and specific binding agents immobilized thereon. The substrates may be used in methods and devices for assaying or isolating analytes in a sample. Also disclosed are methods of making the reactive ion etched surfaces....

20060141509 - Recovery of virus from cell culture using a hypertonic salt solution: A process of harvesting a herpesvirus from a cell culture infected therewith comprises treating said culture with a hypertonic aqueous salt solution to yield a virus suspension, e.g. to give improved yield of virus for live virus vaccine where otherwise cell-disruption might be used to harvest the virus by disrupting...

20060141483 - Stabilization of viral compositions: This invention concerns stabilized virus compositions, preferably a herpesvirus which may be an attenuated or genetically modified herpes simplex virus or varicella zoster virus, and a method of stabilizing viruses and immunizing preparations by the addition of sugars, preferably glucose and amino acids, preferably lysine....

20060141514 - Thermus brockianus nucleic acid polymerases: The invention provides nucleic acids and polypeptides for nucleic acid polymerases from a thermophilic organism, Thermus brockianus. The invention also provides methods for using these nucleic acids and polypeptides....

20060141480 - Use of computationally derived protein structures of genetic polymorphisms in pharmacogenomics and clinical applications: Provided herein are computer-based methods for generating and using three-dimensional (3-D) structural models of target molecules and databases containing the models. The targets can be protein structural variants derived from genes containing polymorphisms. The models are generated using molecular modeling techniques and are used in structure-based drug design studies for...

20060141477 - Use of parvovirus capsid particles in the inhibition of cell proliferation and migration: The invention described herein relates to the discovery of methods and compositions for the inhibition of growth and/or migration of cells that have a receptor that interacts with a parvovirus B19 capsid or fragment thereof (e.g., a P antigen containing cell), including but not limited to, cells of hematopoietic origin...

20060141454 - Use of the slug gene, or of the transcription or expression products thereof in the detection and/or treatment of cancerous cells: Method for the detection of cancerous cells, in particular, mesenchymal tumoral cells, in a biological sample suspected of containing said malignant cells. The methods are based on the aberrant expression of the Slug gene or transcription or expression products thereof. Also disclosed are methods of treatment that based on the...

20060141528 - Compositions and methods for quantification of serum glycoproteins: The invention provides compositions and methods for identifying and/or quantifying glycopolypeptides from human serum or plasma. The compositions and methods include a plurality of standard peptides containing glycosylation sites determined for human serum/plasma proteins....

20060141529 - Compositions, kits and assays containing reagents directed to cortactin and an arg/abl protein kinase: It has been determined Cortactin interacts with an ARG/ABL protein kinase. Thus, provided herein are compositions, kits and assays containing reagents directed to Cortactin and an ARG/ABL protein kinase....

20060141537 - Device and method for separating molecules: An apparatus and method for separating a biomolecule from one or more contaminants in a sample. The apparatus can include a unitary device comprising a plurality of fractionation devices, each reservoir comprising a filter, each reservoir adapted to contain a solid phase, the solid phase adapted to separate the biomolecule...

20060141526 - Genetic marker for coronary artery disease: The present invention describes a Coronary artery disease (CAD) susceptibility gene and the use of this gene in the development of novel therapies to treat CAD....

20060141535 - Hyaluronan receptor for endocytosis: A purified nucleic acid segment encoding a functionally active hyaluronan receptor for endocytosis (HARE) or an active peptide fragment thereof, and methods for producing functionally active HARE or an active peptide fragment thereof therefrom, wherein the functionally active HARE or an active peptide fragment thereof is able to specifically bind...

20060141527 - Method for creating a reference region and a sample region on a biosensor and the resulting biosensor: A method is described herein that can use any one of a number of deposition techniques to create a reference region and a sample region on a single biosensor which in the preferred embodiment is located within a single well of a microplate. The deposition techniques that can be used...

20060141532 - Method for identifying and designing immunogenic peptides: The invention provides a method for identifying peptides for use in increasing a cytotoxic T lymphocytes (CTL) response to an antigen. The method comprises the steps of comparing the amino acid sequence of the VH and/or VL portions of an anti-idiotypic monoclonal antibody to the amino acid sequence of an...

20060141533 - Rapid identification of conditions, compounds, or compositions that inhibit, prevent, induce, modify, or reverse transitions of physical state: The invention concerns arrays comprising hundreds, thousands, to hundreds of thousands of samples and methods for screening thereof. These methods are useful to optimize, select, and discover compounds, compositions, or conditions that prevent, inhibit, induce, modify, or reverse physical-state transitions, particularly in-vivo physical-state transitions relating to disease causing processes. Such...

20060141530 - Reagents and procedures for multi-label high-specificity labeling: A detectable complex and methods for use thereof are provided herein. The detectable complex includes: at least one target material; a first peptide tag bound to the at least one target material; and a second peptide tag bound to the at least one target material. The complex further includes a...

20060141536 - Scintillation proximity test: The invention concerns scintillation proximity assays performed in multiwell plates where a charge coupled device is used to image the wells. Conventional phosphors emit blue light (350-450 nm) which is absorbed by yellow or brown assay components. This problem is addressed by the use of phosphors that emit radiation of...

20060141531 - Ultra-high specificity fluorescent labeling: A self-assembled relay probe for detecting a target material is provided including: a first peptide tag bound to the target material; and a first fluorescent conjugate including a first fluorochrome and a first tag binding group; wherein the first fluorescent conjugate selectively associates with the first tag. The probe further...

20060141525 - Use of lectin library for distinguishing glycoproteins or cells, diagnosing serum or cells, or fractionating glycoproteins or cells: It is intended to provide lectins and a lectin library comprising these lectins which are useful in recognizing differences in carbohydrates on cell surface (for example, sugar chains and cell surface glycoproteins) reflecting extremely subtle differences among cells, etc. and thus distinguishing, identifying or fractionating various cells....

20060141538 - Automatic large-scale screening of cells secreting monoclonal antibodies: The invention relates to an automatic method for the large-scale in vitro screening of cells, secreting at least one specific monoclonal antibody with affinity for a compound of interest, comprising: (10) distribution of antibody-producing cells on at least one culture plate, (12) culturing of said cells, (14) iterative screening of...

20060141540 - Direct selection of cells by secretion product: Cells can be labeled with products which they secrete and release in an efficient manner by coupling the cells at their surface to a specific binding partner for the product and allowing the product to be captured by the specific binding partner as it is secreted and released. The product-labeled...

20060141541 - Method of detecting or diagnosing of a neurodegenerative disease or condition: A neurodegenerative disease or condition is diagnosed in a subject by obtaining a sample of cerebral spinal fluid from the subject and assaying the sample by an assay method that detects the presence of at least one antiphospholipid autoantibody in the sample, wherein an elevated level of at least one...

20060141539 - Miniaturized cell array methods and apparatus for cell-based screening: The present invention discloses devices and methods of performing high throughput screening of the physiological response of cells to biologically active compounds and methods of combining high-throughput with high-content spatial information at the cellular and subcellular level as well as temporal information about changes in physiological, biochemical and molecular activities....

20060141545 - Cancer treatment kits using antibodies to aminophospholipids: Disclosed are the surprising discoveries that aminophospholipids, such as phosphatidylserine and phosphatidylethanolamine, are stable and specific markers accessible on the luminal surface of tumor blood vessels, and that the administration of an anti-aminophospholipid antibody alone is sufficient to induce thrombosis, tumor necrosis and tumor regression in vivo. This invention therefore...

20060141543 - Cellular retinoic acid binding protein ii as a marker for breast cancer: The present invention relates to the diagnosis of breast cancer. It discloses the use of protein cellular retinoic acid binding protein II in the diagnosis of breast cancer. It relates to a method for diagnosis of breast cancer from a liquid sample, derived from an individual by measuring cellular retinoic...

20060141544 - Compositions for binding to assay substrata and methods of using: Compositions and methods for binding to assay substrata in a stable and protective manner, thereby enhancing assay performance, are provided. The compositions comprise lyotropic materials (for example, lyotropic liquid and/or liquid crystalline materials) and may contain macromolecular standards, markers or capture compounds. The compositions are capable of binding to assay...

20060141546 - Bacterial test method by glycated label binding: A method for measuring the bacteria content of fluids such as urine and blood, in which a glycoprotein or glycopeptide is attached to the bacteria and a label attached to or inherent to the glycoprotein or glycopeptide provides a means for determining the amount of bacteria present. A preferred glycoprotein...

20060141542 - Alpha-amylase assay and uses thereof: This invention relates to methods and kits for measuring α-amylase activity in samples such as flour, stock, or amylase concentrate....

20060141548 - Immunoassays for everolimus: Immunoassays for the detection of everolimus are provided. Compounds for producing antibodies for everolimus, as well as antibodies produced therefrom, are also provided....

20060141547 - Novel diagnostic marker, a diagnostic kit and a method for diagnosis of rheumatoid arthritis: The present invention relates to a novel diagnostic marker useful for the diagnosis of rheumatoid arthritis comprising the autoantibodies of mannose binding lectin protein and a process thereof....

20060141549 - Cell-based kinase assay: The present invention relates to improved systems and strategies for the investigation of kinase activity in cells. More specifically, cell-based assay methods are provided that allow the phosphorylating activity of a kinase to be determined inside a cell. The invention also provides cell-based screening assays for identifying compounds that have...

20060141550 - Novel metabolic targets and markers: The present invention provides metabolic markers and targets, especially PEMT activity and metabolic markers and components associated therewith useful for diagnosing and treating various disease conditions....

20060141551 - Analytical method for detecting alkaline sphingomyelinase and kit for use in such method: The use of an alkanoyl L-carnitine for preparing a medicament for treating the erectile dysfunction is described....

20060141552 - Regulation of human caspase-1-like protease: Screening methods to identify candidate therapeutic agents using a newly identified human caspases-1-like protease....

20060141553 - Process for preparing microbial consortium useful as seeding material for bod analysis of pulp and paper industrial wastewater: The present invention provides a seeding material for accurate and reproducible monitoring of BOD load of pulp and paper wastewater, wherein the bacteria are isolated from various locations in India and comprise Micrococcus sp., Staphylococcus sp., Kurthia zopfii, Alcaligenes faecalis, and Pseudomonas aeruginosa, the microbial composition being capable of exerting...

20060141554 - Site-specific labeling of affinity tags in fusion proteins: The present invention provides methods and fluorescent compounds that facilitate detecting and labeling of a fusion protein by being capable of selectively binding to an affinity tag. The fluorescent compounds have the general formula A(B)n, wherein A is a fluorophore, B is a binding domain that is a charged chemical...

20060141555 - Control of biocatalysis reactions: A process for increasing the rate of biocatalysis reactions, which comprises applying a direct current electric field to a reaction mixture, wherein the reaction mixture and the electrodes used to apply said electric field are separated such that the reaction mixture does not come into contact with said electrodes....

20060141556 - Microwave cell lysis method using ionic additives: A cell lysis method in which an ionic additive and microwaves are used is provided. The cell lysis method includes: preparing a sample containing cells to be lysed; adding an ionic additive to the sample; and lysing cells in the sample by radiating microwaves onto the sample in which the...

20060141558 - Bioproduction of astaxanthin using mutant carotenoid ketolase and carotenoid hydroxylase genes: Protein engineered nucleic acid fragments encoding a CrtO ketolase and a CrtZ hydroxylase are provided with increased astaxanthin synthesis activity. Methods using the present nucleic acid fragments are also provided for increasing or altering astaxanthin production in suitable production hosts....

20060141557 - Production of canthaxanthin by phaffia: Disclosed is a process for producing canthaxanthin and echinenone which comprises cultivating a recombinant microorganism which is expressing a β-casotene ketolase gene and belonging to the genus Xanthophyllomyces (Phaffia) in an aqueous nutrient medium under aerobic conditions, and isolating the resulted carotenoids from the cells of said recombinant microorganism or...

20060141559 - Extract from cultured mammalian cell, process for preparation thereof and method of cell-free protein synthesis using the extract: A method for preparing a cultured mammalian cell extract liquid, comprising at least the step of rapidly freezing a cultured mammalian cell suspended in a solution for extraction; a cultured mammalian cell extract liquid prepared by the process; and a method for cell-free protein synthesis using the extract liquid. Preferably,...

20060141561 - Apo-2 ligand/trail variants and uses thereof: The disclosure provides Apo-2 ligand variant polypeptides. Methods of making and chemically modifying Apo-2 ligand variant polypeptides are also provided. In addition, formulations of Apo-2 ligand variant polypeptides are provided. In addition, therapeutic methods for using Apo-2 ligand variant polypeptides are provided....

20060141569 - Compositions and methods for the assay of g-protein coupled receptors and their ligands: A cell comprising one or more endogenous promiscuous G-proteins and an exogenous nucleic acid sequence that encodes a G-protein coupled receptor (GPCR), which optionally comprises a calcium release activated calcium channel (CRAC channel) provides features and improvements that significantly improve membrane level expression of the GPCR, increase signal responses and...

20060141560 - Estrogen receptor genes and utilization thereof: An estrogen receptor gene comprising a nucleotide sequence coding for any of the following amino acid sequences: (a) the amino acid sequence of SEQ ID NO: 1, (b) the amino acid sequence of SEQ ID NO: 4, (c) the amino acid sequence of SEQ ID NO: 23, (d) an amino...

20060141567 - Human chemotactic cytokine: The invention relates to isolated and/or recombinant nucleic acids which encode a human chemotactic cytokine designated human eotaxin, and to isolated and/or recombinant human eotaxin proteins or polypeptides, including synthetic polypeptides. The invention further relates to recombinant nucleic acid constructs, comprising a nucleic acid which encodes a human eotaxin, a...

20060141570 - Intein-mediated protein purification using in vivo expression of an aggregator protein: Purification of recombinant proteins is performed by expressing in a host cell a fusion protein comprising: (a) a product protein domain, (b) an intein, and (c) at least one aggregator protein domain, wherein the aggregator protein domain comprises a protein that is capable of specific association with granules of polyhydroxyalkanoate...

20060141574 - Method for producing monoclonal antibodies: An improved method for the production of monoclonal antibodies is disclosed....

20060141571 - Method for promoting cell growth and increasing the production of the expressed target gene products: The present invention is related to a method to promote cell growth, comprising: (a) cloning one or more aspartase coding sequence into a vector to become a recombinant vector, wherein the recombinant vector including a promoter and one or more aspartase coding sequences manipulated to link and insert into the...

20060141568 - Modification of collagenous materials and medical treatment, diagnosis and monitoring of fibrotic conditions: The present invention relates to the gene PLOD2 which codes for telopeptide lysyl hydroxylase (TLH). This enzyme converts telopeptidyl Lys into telopeptidyl Hyl, that can subsequently be converted into hydroxyallysine cross-links. Collagen with hydroxyallysine cross-links shows a higher resistance to degradation by proteinases than collagen with cross-links derived from allysine....

20060141562 - Mutant e. coli appa phytase enzymes and natural variants thereof, nucleic acids encoding such phytase enzymes, vectors and host cells incorporating same and methods of making and using same: Cells producing mutant phytases having modified activity are provided, as well as the phytases so produced. Also provided are methods of making and producing such phytases and the use of the expressed phytase protein in feed as a supplement....

20060141563 - Mutant protein and refolding method: An FrpB protein having one or more deletions of non-conserved amino acids compared to a corresponding wild-type FrpB protein, and a method of refolding an FrpB protein....

20060141565 - Novel fibroblast growth factor and uses thereof: The present invention generally relates to nucleic acids, proteins, and antibodies. The invention relates more particularly to nucleic acid molecules, proteins, and antibodies of Fibroblast Growth Factor-20 (FGF-22), or its fragments, derivatives, variants, homologs, analogs, or a combination thereof....

20060141573 - Novel tumor necrosis factor receptor homolog and nucleic acids encoding the same: The present invention is directed to novel polypeptides having homology to members of the tumor necrosis factor receptor family and to nucleic acid molecules encoding those polypeptides. Also provided herein are vectors and host cells comprising those nucleic acid sequences, chimeric polypeptide molecules comprising the polypeptides of the present invention...

20060141564 - Nucleic acids encoding duramycin: Nucleic acids encoding preduramycin and produramycin are described, along with recombinant nucleic acids and host cells containing the same and methods of use thereof, such as for the manufacture of the lantibiotic duramycin....

20060141572 - Recombinant light chains of botulinum neurotoxins and light chain fusion proteins for use in research and clinical therapy: Botulinum neurotoxins, the most potent of all toxins, induce lethal neuromuscular paralysis by inhibiting exocytosis at the neuromuscular junction. The light chains (LC) of these dichain neurotoxins are a new class of zinc-endopeptidases that specifically cleave the synaptosomal proteins, SNAP-25, VAMP, or syntaxin at discrete sites. The present invention relates...

20060141575 - Secreted proteins and nucleic acids encoding them: The invention concerns cDNA molecules encoding TANGO 180, TANGO 181, TANGO 182, TANGO 183, TANGO 184, TANGO 185, TANGO 186, TANGO 187, TANGO 188, TANGO 189, and TANGO 215, all of which are either secreted or transmembrane proteins. The nucleic acids and polypeptides of the present invention are useful as...

20060141577 - Selection of host cells expressing protein at high levels: The invention provides a DNA molecule comprising a multicistronic transcription unit coding for i) a polypeptide of interest, and for ii) a selectable marker polypeptide functional in a eukaryotic host cell, wherein the polypeptide of interest has a translation initiation sequence separate from that of the selectable marker polypeptide, and...

20060141576 - Variants of corticotropin releasing hormone receptor type 1 and uses thereof: The present invention identifies four new isoforms of human corticotropin releasing hormone receptor type 1 (CRH-R1e, 1f, 1g and 1h) and three new isoforms of mouse corticotropin releasing hormone receptor type 1 (mCRH-R1c, 1e and 1f). The data indicate that polymorphism of CRH-R1 expression is related to anatomic location, skin...

20060141566 - Virus preparations and methods: Methods are disclosed for the preparation of herpesvirus, such as herpes simplex virus type 2 for vaccine use. Such viruses can be grown on serum free or serum containing media and can be prepared from the virus containing culture supernatant or virus containing cells. The virus is prepared for subsequent...

20060141578 - Method of protein synthesis: The present invention relates to a method of protein synthesis and, more particularly, to a method of effective protein synthesis by regulating the expression of a protein in a host cell, wherein said host cell is transformed with an expression vector comprising a promoter as well as a DNA fragment...

20060141579 - Cytokine zcytor17 ligand polynucleotides: The present invention relates to zcytor17lig polynucleotide, polypeptide and anti-zcytor17 antibody molecules. The zcytor17lig is a novel cytokine. The polypeptides may be used within methods for stimulating the immune system, and proliferation and/or development of hematopoietic cells in vitro and in vivo. The present invention also includes methods for producing...

20060141580 - Interferon-alpha induced gene: The present invention relates to identification of a gene upregulated by interferon-α administration corresponding to the cDNA sequence set forth in SEQ. ID. NO. 1. Determination of expression products of this gene is proposed as having utility in predicting responsiveness to treatment with interferon-α and other interferons which act at...

20060141581 - Il-7 variants with reduced immunogenicity: Modified interleukin-7 (IL-7) polypeptides are disclosed. The modified IL-7 polypeptides have alterations to one or more potential T-cell epitopes, thereby to reduce a T-cell response....

20060141582 - Anti-il-22ra antibodies and binding partners and methods of using in inflammation: The present invention relates to blocking, inhibiting, reduceing, antagonizing or neutralizing the activity of IL-22, IL-20, or both IL-20 and IL-22 polypeptide molecules. IL-20 and IL-22 are cytokines that are involved in inflammatory processes and human disease. IL-22RA (zcytor11) is a common receptor for IL-20 and IL-22. The present invention...

20060141583 - Elaiophylin biosynthetic gene cluster: The present invention relates especially to a DNA fragment that is obtainable from the gene cluster within the genome of streptomycete that is responsible for elaiophylin biosynthesis and that contains at least one gene or a part of a gene that codes for a polypeptide that is involved directly or...

20060141584 - Pretreatment of biomass for ethanol production: A pretreatment of biomass for ethanol production is disclosed. The pretreatment of biomass such as sawdust or wood chips by processing such biomass through an extruder results in a greatly increased recovery of sugar using hydrolysis....

20060141586 - Method for producing l-amino acids using bacteria of the enterobacteriaceae family: There is disclosed a method for producing an L-amino acid, for example L-threonine, L-lysine, L-histidine, L-phenylalanine, L-arginine, L-tryptophan or L-glutamic acid, using a bacterium of the Enterobacteriaceae family, wherein the bacterium has been modified to enhance an activity of L-arabinose permease....

20060141585 - Microorganisms and processes for enhanced production of pantothenate: The present invention features improved methods for the enhanced production of pantoate and pantothenate utilizing microorganisms having modified pantothenate biosynthetic enzyme activities and having modified methylenetetrahydrofolate (MTF) biosynthetic enzyme activities. In particular, the invention features methods for enhancing production of desired products by increasing levels of a key intermediate, ketopantoate,...

20060141587 - Process for the preparation of l-3, 4-dihydroxyphenylalanine by aerobic fermentation of a microorganism: The invention relates to a process for the preparation of L-3,4-dihydroxyphenylalanine wherein L-3,4,-dihydroxyphenylalanine is produced in a fermentation medium by aerobic fermentation of a recombinant microorganism having L-tyro-sine-3-hydroxy-mono-oxygenase activity and at least the metabolic pathways: glycolysis, pentose phosphate pathway, aromatic amino acid pathway, or derivative pathways thereof, which process comprises...

20060141588 - L-glutamic acid-producing microorganism and a method for producing l-glutamic acid: A coryneform bacterium that is modified by using a yggB gene so that L-glutamic acid-producing ability is enhanced as compared to a non-modified strains is cultured in a medium to cause accumulation of L-glutamic acid in the medium or bacterial cells, and L-glutamic acid is collected from the medium or...

20060141589 - Method of producing macrolide compound: The present invention provides a novel method of producing the 12-membered ring macrolide compound 11107D having an antitumor activity by biological transformation. Starting material which is the 12-membered ring macrolide compound 11107B represented by the formula (I) is incubated in the presence of a strain belonging to the genus Mortierella,...

20060141590 - Dna encoding favin-adenine-dinucleotide-dependent-d-erythronate-4-phosphate-de-hydrogenase, pdxr, and microbial production of vitamin b6: The present invention relates to a DNA encoding a novel flavin adenine dinucleotide (FAD)-dependent D-erythronate 4-phosphate (EN4P) dehydrogenase originated from Sinorhizobium meliloti, which is involved in vitamin B6 biosynthesis, and a recombinant microorganism transformed with a vector having the DNA. It also relates to a process for production of vitamin...

20060141591 - Method for producing optically active chroman-carboxylate: In the method for producing an optically active chromancarboxylate of the present invention, one of the enantiomers of racemic chromancarboxylic acid is esterified in a solvent containing an alcohol in the presence of a biocatalyst. After the esterification, the other enantiomer, i.e., the non-reacted chromancarboxylic acid is separated out of...

20060141592 - Process for production of transesterified oils/fats or triglycerides: There are provided polyunsaturated fatty acids-containing oils/fats or triglycerides with high digestion and absorption properties and resistance to oxidative damage, which are suitable for applications in fields such as modified milk for infants, food products and healthy foods and/or supplements, produced by transesterification of polyunsaturated fatty acids-containing oils/fats or triglycerides...

20060141593 - Enzymatic production of (meth) acrylic acid esters: A method for the production of partially esterified (meth)acrylic acid esters of polyalcohols and the use thereof....

20060141594 - Simultaneous anaerobic production of isoamyl acetate and succinic acid: In vivo method of producing esters from acetyle coA, such as isoamyl acetate and succinate, has been developed by producing null mutants in pathways that use acetyl coA and by overexpressing products that use NADH and in order to maintain the proper redox balance between NADH and NAD+. The method...

20060141595 - Process for continuous manufacture of invert sugar syrup and alcohol: The present invention relates to an improved process for the manufacture of invert sugar syrup and alcohol capable of operating in the continuous mode for enzymatic hydrolysis of sucrose or sucrose containing sources for the production of invert syrup and thereby alcohol fermentation and using a recyclable solid catalyst...

20060141596 - Process for the immobilization of enzymes: The present invention relates to a novel process for the immobilization of the enzyme on biocompatible gold nanoparticle encapsulated free-standing membranes. The binding of the enzyme occurs through the amine groups and cysteine residues to the gold nanoparticles. The free-standing gold nanoparticle membrane was synthesized at the interface between chloroform...

20060141597 - Valency platform molecules comprising aminooxy groups: Molecules comprising aminooxy groups are provided, wherein the aminooxy groups provide attachment sites for the covalent attachment of other molecules. In one embodiment, polyoxyethylene molecules comprising aminooxy groups are provided that can be conjugated to wide variety of biologically active molecules including poly(amino acids). In another embodiment, valency platform molecules...

20060141598 - Promotion of peroxisomal catalase function in cells: The molecular mechanisms of peroxisome biogenesis have begun to emerge: in contrast, relatively little is known about how the organelle functions as cells age. The present inventors characterized age-related changes in peroxisomes of human cells and showed that aging compromises peroxisomal targeting signal 1 (PTS1) protein import, with the critical...

20060141599 - Crystals of glucokinase and methods of growing them: Crystalline forms of mammalian Gluckokinase of sufficient size and quality to obtain structural data by X-ray crystallography are presented. Methods of growing such crystals are also disclosed....

20060141600 - Methods and compositions related to argonaute proteins: This invention provides methods and compositions related to Argonaute proteins and, in certain embodiments, the applications of these methods and compositions to treatment and therapeutics based on RNAi....

20060141601 - Egvii endoglucanase and nucleic acids encoding the same: The present invention provides a novel endoglucanase nucleic acid sequence, designated egl7, and the corresponding EGVII amino acid sequence. The invention also provides expression vectors and host cells comprising a nucleic acid sequence encoding EGVII, recombinant EGVII proteins and methods for producing the same....

20060141602 - Packaging of positive-strand rna virus replicon particles: The invention generally relates to recombinant polynucleotides, positive-strand RNA virus (psRNAV) recombinant expression vectors, and packaging systems. The packaging systems are based on the expression of helper functions by coinfecting re-combinant poxvirus vectors comprising recombinant polynucleotides. Methods for obtaining psRNAV replicon particles using these packaging systems are disclosed. Immunogenic compositions...

20060141603 - Methods and compositions for selectively enriching microbes: The present invention provides methods and materials to selectively enrich target microbes in a sample....

20060141605 - Biological neutralization of highly alkaline textile industrial wastewater: The present invention provides a novel process of neutralizing textile industrial wastewater by a bacterial strain isolated in India. The isolated bacterial strain is capable to bring down the pH of wastewater from 12.00 to 7.00 units within two hours. The neutralization of alkaline textile industrial wastewater by such biotechnological...

20060141604 - Biotechnological process for neutralizing alkaline beverage industrial waste water: The present invention provides a process of neutralizing beverage industrial wastewater by a bacterial strain Exiguobacterium sp. isolated in India, which strain is capable of bring down the pH of wastewater from 12.00 to 7.00 units within 1 to 1.5 hours....

20060141606 - Enantioconvergent chemoenzymatic synthesis of (r)-gamma-amino-beta-hydroxybutyric acid ((r)-gabob): The present invention particularly relates to a chemoenzymatic process for the stereoselective preparation of both enantiomers of 3-hydroxy-4-trityloxy butanenitrile key intermediates for the preparation of (R)-GABOB by lipase mediated kinetic resolution of its racemates and their effective application in the enantioconvergent synthesis of (R)-GABOB....

20060141607 - Capillary perfused bioreactors with multiple chambers: A bioreactor for cultivating living cells in a liquid medium. In one embodiment of the present invention, the bioreactor includes a first substrate having a first surface, an opposite second surface and edges. The bioreactor further includes a second substrate having a first surface and an opposite second surface, defining...

20060141609 - Culture observation apparatus, sample tray heat-insulating device and lid: A culture observation apparatus, which is used for observing a cultured cell while culturing the cell, includes a culture device that forms a culture space which is controlled to be an environment suitable for culturing the cultured cell; a sample tray that holds a container housing the cultured cell and...

20060141608 - Piezoelectric sensor arrangement: A piezoelectric sensor arrangement for analysis of fluid samples includes a signal source, a measuring device and a docking system. The sensor arrangement comprises a first part provided with means for receiving a sensor element that exposes a piezoelectric quartz crystal, a second part comprising fluid channels for the sample...

20060141612 - Automated method of manufacturing polymer arrays: The present invention provides methods to process multiple sensors by providing a sensor plate and HT plates. In a preferred embodiment of the invention, methods for assembling microarray pegs and microarray plates are described for high throughput microarray processing....

20060141610 - Microarray devices having controllable reaction volume: This invention relates generally to the field of microarray reaction devices and uses thereof. In particular, the invention a microarray reaction device wherein a plurality of reaction spaces are formed between a first and second plurality of projections, the heights of said plurality of reaction spaces being substantially identical and...

20060141611 - Spatially scanned optical reader system and method for using same: An optical reader system is described herein that uses a scanned optical beam to interrogate a biosensor to determine if a biomolecular binding event occurred on a surface of the biosensor. In one embodiment, the optical reader system includes a light source, a detector and a processor (e.g., computer, DSP)....

20060141613 - Culture observation apparatus: A culture observation apparatus, which is used for observing a cultured cell while culturing the cell, includes a culture device that cultures the cultured cell, and a microscope used for observing the cultured cell. The culture device includes a first space that is controlled to an environment suitable for culture...

20060141614 - Device and method for parallel, automated cultivation of cells in technical conditions: The invention relates to a device for the cultivation of cells in liquid columns measured on a millimetre scale. Said device comprises a container for receiving a liquid culture suspension of the cells, a mixing element for mixing the culture suspension in the container around a mixing axis. The container...

20060141615 - Vegetable alga and microbe photosynthetic reaction system and method for the same: A vegetable alga and microbe photosynthetic reaction system and method for the same is used for vegetable algae and microbe cultivation liquid and algae seeds infused herein to circularly conduct photosynthesis and discharge oxygen; the vegetable alga and microbe photosynthetic reaction system comprises a photosynthetic reaction unit, a pressure liquid...

20060141616 - Cell colonies dissecting and transplanting apparatus: A cell colonies dissecting and transplanting apparatus comprises: a base, a platform, a feeding mechanism having at least three axis feeding means, a movable holder, a cutting means, a transplanting means, and a CCD image capturing means. The platform having the CCD image capturing means therebottom is disposed onto the...

20060141619 - Apparatus and method for machining microchamber for cell culture: There is provided a processing apparatus and a processing method for micro-chamber for cell culture having a desired culturing space. The micro-chamber processing apparatus for cell culture of the present invention comprises a micro-chamber comprising no more than one absorption layer and at least one gel layer, in this order,...

20060141618 - Cell separation apparatus: A cell separation apparatus comprises a means for moving a cell within a cell separating space by applying an electrical voltage to cells in the cell separating space using gel electrodes, and channels for separating and discharging the cell, which thus does not damage a cell sample and prevents the...

20060141617 - Multilayered microcultures: A multilayer microculture capable of modeling complex in vitro structures such as mammalian tissues and organ structures is provided, along with methods for producing such a microculture and methods of using such microcultures for assaying for modulators of cell-cell interaction, cell migration, cell proliferation, cell adhesion or cellular or organismal...

20060141620 - System and method for forming a cardiac tissue construct: A system and method for forming a cardiac tissue construct includes a chamber and cardiac myocytes provided within the chamber. The chamber is arranged to at least partially surround an intact blood vessel in vivo to facilitate formation of the three-dimensional cardiac tissue construct within the chamber....

20060141621 - Process for producing tissue cell from pluripotent stem cell derived from iris pigment epithelial cell of animal and tissue cell obtained by the process: A method for producing tissue cells derived from iris pigmented epithelial cells of an animal, and tissue cells obtained by the method are provided. The method and the tissue cells solve problems such as immunological rejection in cell transplantation, ethical issues, and unbalance between the demand and supply of transplant...

20060141622 - Transplantation of human neural cells for treatment of neurodegenerative conditions: A method of treating neurodegenerative conditions is provided. Neural stem cells may be implanted at and/or remote from a region of neuron degeneration. The methods can include isolating neural stem cells from regions where specific types of neurons corresponding to the neurons to be replaced are generated. The methods can...

20060141623 - Automated tissue engineering system: The invention provides systems, modules, bioreactor and methods for the automated culture, proliferation, differentiation, production and maintenance of tissue engineered products. In one aspect is an automated tissue engineering system comprising a housing, at least one bioreactor supported by the housing, the bioreactor facilitating physiological cellular functions and/or the generation...

20060141624 - Method for transferring molecules in vital cells by means of laser beams and arrangement for carrying out said method: The invention relates to an optical method for targeted transfer of molecules, preferably of DNA, RNA, peptides, amino acids and proteins, into vital cells by means of laser radiation and to an arrangement for implementing the method. The object of the invention, to find a novel possibility for targeted molecule...

20060141625 - Expression cassette and vector for transient or stable expression of exogenous molecules: The disclosure provides an expression cassette and a vector comprising the cassette for expression of a polynucleotide. The expression cassette includes a promoter/enhancer, an intervening region, and a polyadenylation signal domain. Expression systems and methods of using the expression cassette and vector are also provided....

20060141626 - Non-random method of gene shuffling: The present invention concerns the non-random assembling of DNA molecules in a DNA construct and methods of using such constructs, including the production of nucleic acid libraries. The non-random gene shuffling is preferably accomplished by the following steps. First, optionally, the amino acid sequences of proteins encoded by related gene...

20060141627 - Vectors for stable gene expression: The present invention relates to expression vectors capable of promoting transgene expression. The expression vectors include site specific recombination elements, insulator elements, and recombinase elements. In particular, the present invention provides methods for obtaining specific and stable integration of nucleic acids into eukaryotic cells through site specific recombination....

20060134597 - Apparatus and method for rapid separation of cells without using density gradient and antibodies: A method and an apparatus for rapid cell separation without using density gradient and antibody but using a column packed with resin particles. The interactions between cell surfaces and resin particles resulting in the different retention time of different cells in the column contribute to the separation of the specific...

20060134596 - Cryopreservation of human blastocyst-derived stem cells by use of a closed straw vitrification method: An improved method for vitrification of biological cells, especially blastocyst-derived stem cells (BS cells). The method is very mild for the cells that remain viable after they have been thawed. The method comprises, i) transfer of the cells to a first solution (solution A), ii) optionally incubation of the cells...

20060134595 - Polymer affinity matrix, a method for the production and use thereof: A polymer affinity matrix for the binding of one or more substances in a fluid for removing said substance(s) from the fluid and/or decreasing the amount or concentration thereof in said fluid with a view to preventing, eliminating, or reducing undesired activation of components in said fluid is described, as...

20060134598 - Cell culture media dispenser: An apparatus and method of dispensing precise aliquots of cell culture media from a bulk, sterile, flexible container. The apparatus has load cell, a control valve, and a programmable controller. The container suspends from the load cell within the housing. The load cell continuously transmits to the controller an output...

20060134603 - Automated extraction and purification of samples using optical tweezers: An apparatus and method for purifying a forensic sample using an automated extraction and purification system includes optical tweezers; an input channel through which the sample is introduced; a chamber which receives the sample from the input channel; a collection channel through which selected particles of the sample are removed;...

20060134604 - Flexible method and apparatus for high throughput production and purification of multiple proteins: A plurality of proteins of interest, or peptides of interest, or other genetically expressed materials, are screened and subsequently produced using any of a variety of expression systems. The plurality of proteins are extracted from a plurality of separate, processed green juices, each green juice containing one of the proteins...

20060134600 - Method and devices for non-traumatic movement of a probe through biological cell material: A method for moving a probe (10, 91) through a cell material (20), which is formed from biological cells (21), is described, the probe (10) displacing the cells (21) without injury. A probe (10, 91) for performing the method and a cell manipulator, which is equipped with at least one...

20060134602 - Methods of identifying cytotoxic effects in quiescent cells: The present invention provides a system for measuring the cytotoxicity of a test compound against quiescent cells. The inventive methods and reagents may also be used to determine whether a compound, such as a chemotherapeutic agent, will have undesired toxicity toward normal tissues, which generally do not include a significant...

20060134599 - Microfluidic device for cell separation and uses thereof: The invention features methods for separating cells from a sample (e.g., separating fetal red blood cells from maternal method begins with the introduction of a sample including cells into one or more microfluidic channels. In one embodiment, the device includes at least two processing steps. For example, a mixture of...

20060134601 - Osmotic reaction detector for detecting biological and non-biological reactions: A method and apparatus for measuring the presence or absence of interaction between at least a first and second material of interest by measuring osmotic pressure changes in an osmotic cell. Changes in osmotic pressure are determined by measuring the amount of compression exhibited by a compressible, semi-permeable material positioned...

20060134605 - Platelet biomarkers for the detection of disease: The present inventors have surprisingly discovered that platelets sequester angiogenic regulators and prevent their degradation. Thus, by analyzing levels of angiogenic regulators in platelets, it is now possible to detect angiogenic activity, even at an early stage. By monitoring for changes in angiogenic activity, the presence of cancer or other...

20060134608 - Chemically amplified electrochemical detection of affinity reaction: This invention relates generally to the field of electrochemistry. In particular, the invention provides a method for assaying an analyte, using, inter alia, a reactant capable of binding and/or reacting with an analyte to be analyzed on an oxide electrode and a reducing agent not capable of being oxidized directly...

20060134610 - Chris-like virus, compositions, vaccines and uses thereof: A novel virus, the Chris-like virus, has been identified and characterised. Sequences derived from a new paramyxovirus, Chris-like virus (CV), are provided. These sequences encode viral proteins with similarity to recognized viral proteins in other known and characterized paramyxoviruses. The CV viral sequences and the polypeptides encoded therein are useful...

20060134609 - Compositions and methods for determining the presence of sars coronavirus in a sample: The present invention relates to oligonucleotides useful for determining the presence of SARS coronavirus in a test sample. The oligonucleotides of the present invention may be incorporated into detection probes, capture probes and amplification oligonucleotides, or used in various combinations thereof....

20060134611 - Condom device for detecting std's: A condom device for detecting STD's comprising an outer layer having open pores positioned along the surface of the outer layer. The condom device includes an inner layer positioned opposite the outer layer. An intermediate layer is positioned between the outer layer and the inner layer. The intermediate layer receives...

20060134613 - Detection of microbe contamination on elastomeric articles: An elastomeric article that contains a chromogen that undergoes a detectable change in color in the presence of one or more microbes is provided. For example, in one embodiment, the chromogen is a solvatochromic dye (e.g., Reichardt's dye) that undergoes a color change in the presence of bacteria or other...

20060134607 - Marker gene: A method of determining a predisposition to infection, especially infection with HIV, together with therapy for the infection is described....

20060134615 - Method for the simultaneous detection of hpv rna and dna in a sample: A method is provided for the simultaneous detection of human papillomavirus DNA and RNA in a sample....

20060134612 - Methods and compositions for retroviral preintegration complex-based assays: Disclosed are solution-based methods of screening for molecules that modulate the integration of retroviral cDNA into target DNA molecules. These methods can be used to identify molecules that modulate (e.g., inhibit) the integration of unintegrated retroviral cDNA. Such PIC activity modulators may have therapeutic application, for example, to treat or...

20060134614 - Screens for altered immune response capability: The invention relates to associations between genetic variation in the gene encoding CD45 and human disease and human immune responses. In particular the invention provides methods of screening human subjects for the presence of an “altered immune response capability”, which may in turn affect susceptibility to viral disease and/or autoimmune...

20060134616 - Sonication to selectively lyse different cell types: A sonication apparatus is directed to a microfluidic-based system to automate differential extraction of specific cell types within a mixed sample. The microfluidic-based system includes a sonication module for selective cell lysis, separating means to eliminate centrifugation, high surface area pillar chip modules to purify DNA from a cell lysate,...

20060134606 - Substrates for isolating reacting and microscopically analiyzing materials: An immobilizing device for biological material comprises a rigid support (12) carrying a substrate layer (20, 20′) of polymer having biological immobilizing properties, e.g. for amino and nucleic acids. Substantially solid ultra-thin substrate layers (20′) having a thickness less than about 5 micron, preferably between about 0.1 and 0.5 micron,...

20060134654 - Adam12, a novel marker for abnormal cell function: The present invention provides a method, an assay and a kit for providing an indication of abnormal cell function. It was surprisingly found that the change in the serum ADAM12 concentration in individuals was useful as a prognostic tool to predict the clinical outcome, complications and mortality following an abnormal...

20060134660 - Alien sequences: The present invention provides sequences and reagents for preparing microarrays with internal controls. Specifically, the present invention defines and provides sequences that are not present in the hybridizing mRNA or cDNA, and therefore can be used both as hybridization controls and for inter-spot normalization....

20060134622 - Amplified cancer target genes useful in diagnosis and thereapeutic screening: A gene useful as a prognostic indicator of survival of patients afflicted with cancers such as breast cancer is described. Also disclosed are methods for screening potential therapeutic agents, such as antitumor agents, based on their modulation of the expression of a TRIP13 gene, or splice variants thereof, along with...

20060134644 - Apparatus and methods for detecting target analyte: This invention relates to apparatus and methods to detect a target analyte in a test sample by forming a fluorescent complex comprising the target analyte and a probe. The fluorescence decay and/or lifetime changes upon complex formation. The apparatus includes a pulsed light source and a digitizer to measure fluorescent...

20060134649 - Bardet-biedl susceptibility gene and uses thereof: The present invention relates to the designation of ADP-ribosylation factor-like 6 as the BBS3 gene, that is involved in the genetic disease Bardet Biedl Syndrome (BBS), which is characterized by such diverse symptoms as obesity, diabetes, hypertension, mental retardation, renal cancer and other abnormalities, retinopathy and hypogonadism. Methods of use...

20060134643 - Bisulfite conversion of dna: The present invention provides an improved method for the bisulfite conversion of DNA, and facilitates the analysis of cytosine methylation of genomic DNA. Novel combinations of denaturing solvents, new reaction conditions and new purification methods provide surprisingly efficacious methods for bisulfite conversion of DNA relative to prior art methods. The...

20060134619 - Casein kinase i epsilon and casein kinase i delta and sleep in humans: Genetic variants of the casein kinase I delta and casein kinase I epsilon genes are disclosed. Herein, two novel mutant casein kinase I delta genes and a novel mutant casein kinase I epsilon gene are disclosed. The protein products of the mutant casein kinase I delta gene and the mutant...

20060134618 - Detection, identification and differentiation of eubacterial taxa using a hybridization assay: The present invention relates to a method for the specific detection and/or identification of Enterococcus species, in particular Enterococcus faecalis and/or Enterococcus faecium, using new nucleic acid sequences derived from the ITS (internal Transcribed Spacer) region. The present invention relates also to said new nucleic acid sequences derived from the...

20060134673 - Diagnostic assay for wiskott-aldrich syndrome and genetically related disorders: The methods and compositions of the invention find use in the clinical diagnosis of primary immunodeficiencies, particularly Wiskott-Aldrich related syndromes. The compositions of the invention include isolated nucleic acid molecules and oligonucleotide pairs suitable for use in amplifying regions of the Wiskott-Aldrich syndrome protein gene and in determining the nucleotide...

20060134621 - Diagnostic test for attention deficit hyperactivity disorder: A two-pronged approach useful for diagnostic testing of ADHD is provided, comprising (1) a genetic test to determine the DRD4 genotype and (2) a test to characterize dopamine release to mental/physical/pharmacological probes....

20060134653 - Differential expression of genes in microsatellite instability: The present invention relates to nucleic acid sequences which are overexpressed in colorectal tumors which display a high level of microsatellite instability (MSI-H) vs. tumors displaying a low level of microsatellite instability (MSS), and can thus be used to identify MSI-H in a patient, and be used further to facilitate...

20060134633 - Double ended sequencing: This invention relates to methods of sequencing DNA. More specifically, this invention relates to methods of sequencing both the sense and antisense strands of DNA through the use of blocked and unblocked sequencing primers. In brief, these methods include the steps of annealing an unblocked primer to a first strand...

20060134670 - Enabling tools to identify ligands for hormone nuclear receptors: A method and kit developed to identify substances that act as ligands, corepressors, coactivators, agonist and antagonists for cloned nuclear hormone receptors, as well as a test kit for use in the methods is provided herein. More specifically, the method involves expressing a nuclear hormone receptor, receptor heterodimer, and/or receptor...

20060134617 - Enzymatic method for the isolation of dna from plant tissue: The invention relates to a mixture of cell wall degrading enzymes. The invention further relates to methods for isolating DNA from plant tissue that utilize a mixture of cell wall degrading enzymes. The invention also relates to kits for isolating DNA from plant tissue wherein the kit comprises a mixture...

20060134638 - Error reduction in automated gene synthesis: In embodiments of the present invention, methods are provided for removing double-stranded oligonucleotide (e.g., DNA) molecules containing one or more sequence errors, generated during nucleic acid synthesis, from a population of correct oligonucleotide duplexes. In one embodiment, the oligonucleotides are generated enzymatically. Heteroduplex (containing mismatched bases) oligonucleotides may be created...

20060134688 - Genes overexpressed in prostate disorders as diagnostic and therapeutic targets: Disclosed are methods for diagnosing, monitoring the progression of, and treating a prostate disorder based upon genes that are differentially expressed in prostate disorders. Also disclosed are methods for identifying agents useful in the treatment of a prostate disorder, methods for monitoring the efficacy of a treatment for a prostate...

20060134680 - Homogeneous populations of nucleic acids, methods of synthesyzing same and uses thereof: A homogeneous population of fully double stranded nucleic acid molecules having blunt ends, each of the molecules being at least 100 base pairs long, and having the same repetitive core sequence selected from the group consisting of mono, di-, or tri-nucleotide, with the proviso that the repetitive core sequence is...

20060134634 - Human gene: The present invention provides novel human genes, for example a novel human gene comprising a nucleotide sequence coding for the amino acid sequence shown under SEQ ID NO:1. The use of the genes makes it possible to detect the expression of the same in various tissues, analyze their structures and...

20060134664 - Identification of dysregulated genes in patients with neurological diseases: The present invention identifies a number of gene markers whose expression is altered in neurodegenerative diseases (ND). These markers can be used to diagnose or predict ND in subjects, and can be used in the monitoring of therapies. In addition, these genes identify therapeutic targets, the modification of which may...

20060134640 - Inhibiting the growth of bacterial biofilms: The present invention provides targets and methods for inhibiting the development, formation and/or maturation of bacterial biofilms, and for detecting bacterial biofilms....

20060134672 - Macromolecular arrays on polymeric brushes & methods for preparing the same: Polymeric brush substrates and methods for their preparation are provided. Methods are also provided for preparing macromolecular arrays on such polymeric brush substrates. Using polymeric brush substrates allows control over functional site density as well as wettability and porosity of the substrate. These polymeric brushes are useful in solid-phase synthesis...

20060134620 - Method and apparatus for concentration and purification of nucleic acid: In a method for concentration and purification method of nucleic acids using electrophoresis, cationic surfactant is added to a sample containing nucleic acids so as to adjust electric charge of impurities in the sample, and then the sample is placed in an electric field for electrophoresis so as to concentrate...

20060134662 - Method and system for genotyping samples in a normalized allelic space: Aspects of the present invention describe an apparatus and method for generating genotype calls for a sample. The genotyping initially models allelic signal response into an allelic model having one or more model parameters for an identified one or more sources of systematic variation. The model and parameters are then...

20060134628 - Method for assembly of a polynucleotide encoding a target polypeptide: The present invention provides a novel approach to utilizing the results of genomic sequence information by computer-directed polynucleotide assembly based upon information available in databases such as the human genome database. Specifically, the present invention can be used to select, synthesize and assemble a novel, synthetic target polynucleotide sequence encoding...

20060134659 - Method for demethylating target methylated region of genome dna: It is an object of the present invention to provide a demethylation method that, from among multiple methylated regions present in the genome DNA of a living cell, can demethylate a specified methylated region only without influencing the other methylated regions, and to achieve the object, a target methylated region...

20060134667 - Method for detecting fusion gene: The present invention relates to a method for detecting fusion gene transcripts resulting from chromosomal translocation. Specifically, the method of the present invention comprises allowing at least two or more probes, each of which contains a partial base sequence of exons which sandwich the breakpoint of a fusion gene or...

20060134625 - Method for determining susceptibility to schizophrenia: The present invention relates to methods for identifying the susceptibility or predisposition of an individual to schizophrenia SZ) or to clusters of symptoms associated thereof. Particularly, the tests can be performed before or after the disorders appear. More particularly, the present invention relates to the determination of epistatic effect of...

20060134647 - Method for elimination of false reactivities in immunoassay for hepatitis a virus: An ancillary reducing reagent composition is used to significantly reduce and substantially eliminate the incidence of false positive results in an immunoassay for the detection of antibodies to Hepatitis A virus. The reducing agent contained in the ancillary reducing reagent composition is a sulfhydryl compound buffered to a pH sufficient...

20060134630 - Method for screening for an antimicrobial polypeptide: The present invention relates to a method for screening polynucleotide sequences encoding antimicrobial polypeptides and methods for testing the antimicrobial activity of an antimicrobial polypeptide....

20060134637 - Method for the detection of cancer related gene transcripts in blood: The present invention is directed to detection and measurement of gene transcripts and their equivalent nucleic acid products in blood. Specifically provided is analysis performed on a drop of blood for detecting, diagnosing and monitoring diseases using gene-specific and/or tissue-specific primers. The present invention also describes methods by which delineation...

20060134635 - Method for the detection of coronary artery disease related gene transcripts in blood: The present invention is directed to detection and measurement of gene transcripts and their equivalent nucleic acid products in blood. Specifically provided is analysis performed on a drop of blood for detecting, diagnosing and monitoring diseases using gene-specific and/or tissue-specific primers. The present invention also describes methods by which delineation...

20060134639 - Method for the determination of cellular transcriptional regulation: The present invention relates to a new method for determining the RNAi mediated transcriptional regulation of a cell by the determination of a pattern of at least 3 miRNA detected simultaneously and quantified in the same cell extract. The determination of a pattern of miRNA comprises the steps of: (i)...

20060134646 - Method for treatment of hiv infection: A method for treatment of HIV infection includes administering at least one anti-HIV drug, such as a reverse transcriptase inhibitor, to a patient in need of such treatment and administering an extract from inflammatory tissue inoculated with vaccinia virus to the patient following the administration of the at least one...

20060134655 - Method of detection and quantification of cgmp by using a cgmp-visualizing probe: As a cGMP-visualizing probe capable of detecting and quantifying cGMP easily and accurately even in vivo and for a method of detecting and quantifying cGMP by using the same, a cGMP-visualizing probe comprising a polypeptide, which binds specifically to cGMP, and two chromophores having different fluorescence wavelengths each linked respectively...

20060134675 - Method of isolating and purifying nucleic acids using immobilized hydrogel or peg-hydrogel copolymer: Provided is a method of isolating and purifying nucleic acids using an immobilized hydrogel or polyethylene glycol (PEG)-hydrogel copolymer. The method includes: immobilizing a functional group-containing hydrogel or PEG-hydrogel copolymer on a substrate; adding a mixed sample solution containing a salt and nucleic acids to the hydrogel- or PEG-hydrogel copolymer-immobilized...

20060134679 - Methods and compositions for acquiring information from unstretched polymer conformations: The invention relates to the methods for analyzing polymers that are in unstretched conformations. In particular, the polymers such as nucleic acids may be present in hairpin conformations....

20060134629 - Methods and compositions for elucidating protein expression profiles in cells: The present invention relates generally to methods and compositions for the identification of differential protein expression patterns and concomitantly the active genetic regions that are directly or indirectly involved in different tissue types, disease states, or other cellular differences desirable for diagnosis or for targets for drug therapy....

20060134683 - Methods and devices for hybridization and binding assays using thermophoresis: Device and methods for performing hybridization and binding assays are described....

20060134652 - Methods and kits for preparing nucleic acid samples: The present invention provides methods for preparing nucleic acid samples. The methods of the present invention are particularly amenable for preparing samples that substantially represent the whole transcripts. The method is particularly suitable to use with microarray based expression analysis....

20060134671 - Methods and systems for prognosis and treatment of solid tumors: The present invention provides methods, systems and equipment for the prognosis and treatment of renal cell carcinoma (RCC) or other solid tumors. Genes prognostic of clinical outcomes of a solid tumor can be identified according to the present invention. The expression profiles of these genes in peripheral blood mononuclear cells...

20060134665 - Methods for analyzing transcripts: In one aspect of the invention, high density arrays are used with 5′ and 3′ RACE (rapid amplification of cDNA ends) or RAGE (rapid amplification of Genomic DNA) in tandem to detect and characterize transcripts or genomic structures....

20060134666 - Methods for detecting nucleic acid analyte: The present invention is directed to a method of sequencing a target nucleic acid molecule having a plurality of bases. In its principle, the temporal order of base additions during the polymerization reaction is measured on a molecule of nucleic acid, i.e. the activity of a nucleic acid polymerizing enzyme...

20060134678 - Methods for drug discovery disease treatment, and diagnosis using metabolomics: The small molecule profiles of cells are compared to identify small molecules which are modulated in altered states. Cellular small molecule libraries, methods of identifying tissue sources, methods for treating genetic and non-genetic diseases, and methods for predicting the efficacy of drugs are also discussed....

20060134676 - Methods for drug discovery, disease treatment, and diagnosis using metabolomics: The small molecule profiles of cells are compared to identify small molecules which are modulated in altered states. Cellular small molecule libraries, methods of identifying tissue sources, methods for treating genetic and non-genetic diseases, and methods for predicting the efficacy of drugs are also discussed....

20060134677 - Methods for drug discovery, disease treatment, and diagnosis using metabolomics: The small molecule profiles of cells are compared to identify small molecules which are modulated in altered states. Cellular small molecule libraries, methods of identifying tissue sources, methods for treating genetic and non-genetic diseases, and methods for predicting the efficacy of drugs are also discussed....

20060134674 - Methods for identifying dna copy number changes: Methods of identifying allele-specific changes in genomic DNA copy number are disclosed. Methods for identifying homozygous deletions and genetic amplifications are disclosed. An array of probes designed to detect presence or absence of a plurality of different sequences is also disclosed. The probes are designed to hybridize to sequences that...

20060134668 - Methods for treating patients and identifying therapeutics: The disclosure provides, among other things, molecular markers for categorizing the neoplastic state of a patient and methods for using the molecular markers in designing, screening and targeting therapeutic agents for the treatment of colon cancer....

20060134681 - Methods of assaying for compounds that inhibit premature translation termination and nonsense-mediated rna decay: The present application provides methods of assaying for compounds that inhibit premature translation termination and nonsense mediated RNA decay in cells....

20060134650 - Methylation-sensitive restriction enzyme endonuclease method of whole genome methylation analysis: The invention provides methods of identifying a plurality of reactive recognition sites for a restriction endonuclease in genomic DNA. In particular embodiments, the methods can be used to identify methylation state of a plurality of CpG target sites in genomic DNA. The method can include steps of treating genomic DNA...

20060134687 - Microfluidic differential extraction cartridge: A differential extraction system is directed to a microfluidic-based integrated cartridge to automate differential extraction of specific cell types within a mixed sample. The integrated cartridge includes a sonication module for selective cell lysis, separating means to eliminate centrifugation, high surface area pillar chip modules to purify DNA from a...

20060134657 - Nanoscale electronic detection system and methods for their manufacture: A new, extremely sensitive, and rapid electronic detection method for direct detection of hybridized genomic targets to specific probes on the microarray is proposed. The method consists of fast electronic accumulation of the DNA target on a particular electrode site at the micro-electrode array, sequential electronic hybridization of oligonucleotide labeled...

20060134645 - Notch1 variants associated with cardiovascular disease: Human individuals at increased risk for vascular pathology are identified by determining whether the individuals' genome comprises a Notch1 allele predetermined to be associated with an increased risk for vascular pathology. Notch1 alleles are identified as being associated with increased risk for vascular pathology by detecting the presence of a...

20060134623 - Novel compositions and methods in cancer: The present invention relates to novel sequences for use in detection, diagnosis and treatment of cancers, especially lymphomas. The invention provides cancer-associated (CA) polynucleotide sequences whose expression is dys-regulated in cancer tissues. The present invention provides CA polypeptides associated with cancer and provides diagnostic compositions and methods for the detection...

20060134631 - Novel dna glycosylases and their use: Novel cytosine-, thymine- and uracil-DNA glycosylases, subcellular localization peptides, nucleic acid molecules containing the same, methods of identifying such enzymes and their use in various methods including mutagenesis, cell killing and DNA sequencing and modification, are desired....

20060134651 - Nuclear localization signal of lentiviral integrase and methods of use thereof: The present invention provides novel peptides, nucleic acids, vectors, compounds, compositions and methods for regulating nuclear import The present invention also relates to a lentiviral NLS, and methods of use thereof for inhibiting HIV pathogenesis and disease progression, and for gene delivery methods...

20060134632 - Oligonucleotides and methods for detecting hepatitis c viral nucleic acids: The present invention provides methods and compositions for determining the presence and/or amount of HCV nucleic acids in a test sample. In particular, substantially purified oligonucleotide primers and probes are described that can be used for qualitatively and quantitatively detecting HCV nucleic acid in a test sample by amplification methods....

20060134648 - One-tube nested pcr for detecting mycobacterium tuberculosis: The present invention relates to a kit and method for detecting M. tuberculosis of suspected patient. The present invention also relates to primers and probe used to detect M. tuberculosis by performing one tube nested PCR....

20060134642 - Pharmacological targeting of bacterial dna ligase for treatment and prevention of bacterial infections: This invention provides methods for the discovery of molecules that target an essential step of bacterial DNA replication—the sealing of DNA strands by NAD+-dependent DNA ligase. Specific structural components of NAD+-dependent DNA ligase that are important for the reaction of DNA ligase with NAD+ and that comprise a putative binding...

20060134669 - Plasmon resonance biosensor and method: A method of analyzing a sample comprising directing a beam of light to a spot on the surface of a microarray treated with the sample using a digital micromirror device, and observing the SPR spectral shift due to a chemical binding event. The digital micromirror device can be, for example,...

20060134624 - Polymerase-based protocols for the introductions of deletions and insertions: The invention relates to primers, libraries of primer, kits and methods for site-specific in vitro mutagenesis comprising: (a) cloning a parental polynucleotide into a vector comprising a cloning site, thereby obtaining a cloned product; (b) denaturing the cloned product, thereby obtaining a single-stranded polynucleotide template; (c) hybridizing at least one...

20060134682 - Preparation and use of superior vaccines: This invention provides an isolated population of polynucleotides comprising or corresponding to at least one polynucleotide shown in Table 1 and their respective complements. It also provides a polynucleotide encoding a ligand or antibody or engineered protein that binds to a cell surface protein of an antigen presenting cell and...

20060134627 - Primers and probes for amplification and detection of human inhibitor of dna-binding: Oligonucleotide primers and polynucleotide probes provide selective amplification and detection of Id genetic sequences and are selected both (1) to provide the desired specificity so that they amplify only the specific Id type to which they are targeted; and (2) to introduce terminal restriction endonuclease cleavage sites into the amplicon...

20060134626 - Process for extracting nucleic acid: The invention provides a process and kit for isolating and purifying nucleic acids such as DNA or RNA or a hybrid molecule of DNA and RNA. A siliceous material combined with a solution is used to prepare highly pure nucleic acids, especially DNA. The siliceous material is a support for...

20060134689 - Real-time polymerase chain reaction-based genotyping assay for single nucleotide polymorphism: The present invention provides fluorescence-based real-time PCR assays for the rapid detection of single nucleotide polymorphisms (SNPs). The genotyping assay can be used to detect SNPs of a number of genes of interest that include, but are not limited to, the human multidrug resistance gene (MDR1) single nucleotide polymorphisms C3435T...

20060134656 - Reduction of non-specific adsorption of biological agents on surfaces: The present invention relates to surface-modified substrates that demonstrate reduced non-specific adsorption of biological agents. The substrates are silicon or carbon substrates having ethylene glycol oligomers covalently bound to at least one substrate surface. The substrates may be used in sensor devices, such as biochips, and in implantable medical devices...

20060134636 - Standardization of growth conditions for human embryonic stem cells intended for use in regenerative medicine: This disclosure provides a system for qualifying embryonic stem cells intended for human therapy. A comprehensive sequencing project has identified important markers that are characteristic of undifferentiated pluripotent cells. Combinations of these markers have been used to screen feeder cells, media additives, and culture conditions that promote rapid expansion of...

20060134684 - Sulfotransferase sequence variants: Isolated sulfotransferase nucleic acid molecules that include a nucleotide sequence variant and nucleotides flanking the sequence variant are described, as well as sulfotransferase allozymes. Methods for determining if a mammal is predisposed to thyroid disease or cancer also are described....

20060134663 - Transcriptome microarray technology and methods of using the same: Arrays containing a transcriptome of a diseased tissue and methods of using the arrays for diagnosis, prognosis, screening, and identification of disease are provided herein. The transcriptome arrays from diseased tissue are useful for diagnosis of a disease by analysis of the genetic profile of a tissue sample specific to...

20060134641 - Treating viral infections with krill enzymes: The invention relates to a multifunctional enzyme that can be derived from crustaceans or fish. The enzyme has at least one of a chymotrypsin, trypsin, elastase, collagenase and exo peptidase activity, and a molecular weight between about 20 kd and about 40 kd as determined by SDS PAGE. Preferably, the...

20060134661 - Treatment of cancer and compositions: The invention discloses a method of identifying a gene associated with stage III primary cancer or lymph node metastasis. The genes so identified include CAV1, CST3, LIMK1, MMP2, MMP15, VEGF, ETV4, MMP9, PIK3C2B, and SERPIN1. Also disclosed are methods for diagnosis, prognosis, and treatment of cancer. The invention further discloses...

20060134686 - Unary avian myeloblastosis virus reverse transcriptase and its use: An avian myeloblastosis virus reverse transcriptase composition which comprises a heterodimer consisting of an α subunit having a molecular weight of 68000 and a β subunit having a molecular weight of 92000 in an amount of at least 80%....

20060134685 - Use of a biochip for the diagnosis of sepsis or sepsis related syndrome: The application relates to the use of a nucleic acid and/or protein chip comprising probe nucleic acids and/or probe proteins, which are specific for cellular stress, inflammatory and immune reactions, associated with stress, inflammatory and immune reactions, induced during acute phase responses or any combination thereof and which are immobilised...

20060134658 - Use of rna trans-splicing for generation of interfering rna molecules: Methods and compositions for generating novel nucleic acid molecules through trans-splicing that function to reduce the level of expression of a target RNA. The compositions of the invention include pre-trans-splicing molecules (PTMs) designed to interact with a target precursor messenger RNA molecule (target pre-mRNA) and mediate a trans-splicing reaction resulting...

20060134695 - Biosensor surface structures and methods: A biosensor surface with a low density of ligand-carrying tether molecules on a base layer. Also, surface plasmon resonance (SPR) devices have the biosensor surface attached to a thin gold layer with backside angle-spread incident radiation for resonance excitation and reflective detection....

20060134699 - Inter-alpha trypsin inhibitor biopolymer marker indicative of insulin resistance: The instant invention involves the use of a combination of preparatory steps in conjunction with mass spectroscopy and time-of-flight detection procedures to maximize the diversity of biopolymers which are verifiable within a particular sample. The cohort of biopolymers verified within such a sample is then viewed with reference to their...

20060134700 - Inter-alpha trypsin inhibitor biopolymer marker indicative of insulin resistance: The instant invention involves the use of a combination of preparatory steps in conjunction with mass spectroscopy and time-of-flight detection procedures to maximize the diversity of biopolymers which are verifiable within a particular sample. The cohort of biopolymers verified within such a sample is then viewed with reference to their...

20060134691 - Labeling methodology comprising oligopeptides: A method for providing a labeled target protein or labeled target peptide in high purity, which method comprises the steps of a. contacting a chemical molecule, which chemical molecule comprises a target protein or a target peptide residue beside amino acid residues, spacer residues, and/or linker residues, an affinity tagging...

20060134697 - Method of preparing coded compound libraries: The present invention relates to methods of preparing a library of compounds, wherein each of the compounds resides on the exterior portion and is encoded by coding tags confined in each of successive zones on the interior portion of a solid support. Following screening of the compounds, the coding tags...

20060134698 - Methods for treating cardiac disease by modifying an n-terminal domain of troponin i: The invention provides methods for identifying modulators of cardiac troponin I activity as well as methods for using N-terminally truncated forms of cTnI to monitor the function of the circulatory system in general, and the heart in particular, of an organism such as man. Additionally, the invention provides methods of...

20060134694 - Methods of protein profiling by thiolation: Methods for analyzing a protein are provided, including isolating the protein having original reactive functional groups from a biological sample, and converting substantially all original reactive functional groups into thiol groups, followed by attaching tags to the protein via the thiol groups....

20060134693 - Olfactory cyclic nucleotide-gated channel cell-based assays to identify t1r and t2r taste modulators: Screening assays, preferably high throughput, are provided that screen libraries of candidate compounds to identify agonists, antagonists, enhancers or modulators of taste receptors (bitter, sweet or savory (umami) taste receptor) using test cells that co-express at least one functional taste receptor and an olfactory cyclic nucleotide-gated channel (oCNGC). (The oCNGC...

20060134690 - Process for the purification of interleukin-4 and its muteins: This invention relates generally to a method for purifying Interleukin-4 or related variants from an Escherichia coli culture medium, where Interleukin-4 and its derivatives are expressed as insoluble protein aggregates, so-called inclusion bodies....

20060134696 - Small molecule substrate based enzyme activity assays: The invention relates to a method of high throughput chemical analysis comprising the steps of combining one test compound with a solution comprising m enzyme(s) and n substrate(s), wherein m is an integer equal to one or greater, n is an integer equal to one or greater, and m+n≧3, incubating...

20060134692 - Target activated microtransfer: A method of removing a target from a biological sample which involves placing a transfer surface in contact with the biological sample, and then focally altering the transfer surface to allow selective separation of the target from the biological sample. In disclosed embodiments, the target is a cell or cellular...

20060134706 - Atopic dermatitis inducer: An atopic dermatitis inducer binding to a human own IgE antibody and activating mast cells and basophiles, which includes a purified human secretion fraction, or an antigenic molecule or an antigenic determinant in the purified fraction, and obtained through the following steps of: filtering a human secretion, removing insoluble matters...

20060134705 - Cvd assay: An assay method for the detection of potential for CVD or propensity to CVD in a human or non-human animal subject, said method comprising assessing the concentration of calprotectin in a calprotectin-containing sample taken from said subject....

20060134704 - Microwell array chip for detecting antigen-specific lymphocytes, method of detecting and method of manufacturing antigen-specific lymphocytes, and method of cloning antigen-specific lymphocyte antigen receptor genes: A microwell array chip that has multiple microwells and is employed to contain a single lymphocyte specimen in each microwell and detect antigen-specific lymphocytes in single units; wherein the microwell array chip is of a shape and of dimensions where only one lymphocyte is contained in each microwell. A method...

20060134707 - Monoclonal and polyclonal antibodies to equine hemoglobin and apparatus and methods using the antibodies and/or peroxidase reactions in the identification and localization of ulcers in equines: A diagnostic and testing apparatus and related methods for the use of the same are disclosed which derive and use antibodies to equine globin and hematin in testing apparatus, kits, and methods for detecting and localizing gastric and colonic ulcers in horses. Fecal droppings from a horse to be tested...

20060134708 - Detection and treatment of renal cancer: The present invention relates to compositions and methods for cancer therapies and diagnostics, including but not limited to, cancer markers. In particular, the present invention provides cancer markers associated with specific cancers and diagnostic assays for the detection of such markers as indicative of the presence of kidney and urothelial...

20060134709 - Engineering fc antibody regions to confer effector function: The present invention relates to molecules having a variant Fc region, wherein said variant Fc region comprises at least one amino acid modification relative to a wild-type Fc region. These modified molecules confer an effector function to a molecule, where the parent molecule does not detectably exhibit this effector function....

20060134713 - Biosensor apparatus and methods of use: Disclosed herein are methods and devices for detecting the presence of an analyte of interest. A biosensor device can include a reaction chamber and an electrochemical detection chamber. The reaction chamber can include at least one immobilized binding site and a probe conjugate adapted to bind to at least one...

20060134711 - Methamphetamine derivatives and conjugates for immunoassay: s

20060134712 - Multiplexed assay methods: The present invention is directed to methods for conducting multiplexed assays. The methods are particularly well suited for measuring a plurality of analytes that may be present in very different abundances. The invention also relates to systems, devices, equipment, kits and reagents for use in such methods....

20060134710 - Wdr11 protein assay: An affinity-based analyte separation method of assaying a sample for a WDR11 protein. The method can be coupled to a variety of detection methods, including detection of an observable label or detection using mass spectrometry. The methods may be applied to assessing cancerous cells....

20060134714 - Detection and identification of peptide and protein modifications: Embodiments of the present invention provide devices and methods for detecting, identifying, distinguishing, and quantifying modification states of proteins and peptides using Surface Enhanced Raman (SERS) and Raman spectroscopy. Applications of embodiments of the present invention include, for example, proteome wide modification profiling and analyses with applications in disease diagnosis,...

20060134716 - Electrospun enzyme-nanocomposite biosensing material: The present invention provides biosensing material comprising polymer-enzyme nanocomposite fibers. A biosensor comprising such material may be obtained through an electrospinning process to yield a nonwoven mat, which retains enzyme activity. The large amount of available surface area obtained by the methods of the present invention provides unusually high sensitivity...

20060134715 - Optically detectable organophosphonates: in which X represents an optically detectable moiety, n is an integer with 1≦n≦20, R1 is an unbranched or branched alkyl group having 1 to 20 carbon atoms, and R2 is hydrogen or —CH2—O—R3 group, wherein R3 has the same meaning as R1. These compounds are useful as inhibitors of...

20060134718 - Assay for camp based on exclusive binding of camp to the b-binding sites of camp dependent protein kinase (capk): The present invention provides a method for assaying for cAMP in a sample, said method comprising contacting a sample with an unknown cAMP content with a polypeptidic cAMP binding agent and optionally with a labelled cAMP and detecting conjugates of cAMP or labelled cAMP and said binding agent, characterized in...

20060134717 - Method for making biochips: A method for immobilizing biological polymers such as DNA or proteins, on a solid support, by ionocovalent bond, for making biochips, and the resulting chips obtained by the method....

20060134721 - Affinity trap reactor and single-step process for purifying angiostatin-like fragment from human plasma using the same: The present invention provides an affinity trap reactor that enables the reaction between an enzyme bound to a support and substrate to proceed efficiently without its applications being restricted by the type of enzyme and substrate used. The present invention relates to an affinity trap reactor composed of a support...

20060134722 - Method and compositions for detecting botulinum neurotoxin: A molecular construct capable of fluorescent resonance energy transfer (FRET), comprising a linker peptide, a donor fluorophore moiety and an acceptor fluorophore moiety, wherein the linker peptide is a substrate of a botulinum neurotoxin selected from the group consisting of synaptobrevin, syntaxin and SNAP-25, or a fragment thereof capable being...

20060134720 - Method for analyzing c-terminal amino acid sequence of peptide using mass spectrometry: The present invention provides a method for analyzing the C-terminal amino acid sequence of a peptide by using a reaction for successively releasing the C-terminal amino acids of the peptide, which method can suppress, when successively releasing the C-terminal amino acids of a peptide of long amino acid length, such...

20060134723 - N-or c-terminal peptide selection method for proteomics: The present invention provides a new and improved method for quantitative determination and/or identification of proteins in a sample. In one aspect, the invention provides a mass spectroscopic method for comparing protein levels in two or more samples by differentially isotopically labeling each sample's proteins' N- or C-termini. In another...

20060134719 - Novel serine protease: It is disclosed that a novel polypeptide useful for screening for an agent for specific diseases to which gastrointestinal hormones are related, particularly irritable bowel syndrome, a polynucleotide encoding the polypeptide, a vector comprising the polynucleotide, and a transfected cell by the vector. It is disclosed that the polypeptide is...

20060134724 - Method and probe for identifying bacterial virulence modifying agents, agents thus identifed, and their use: A method for identifying antibacterial agents comprises depleting bacteria of a strain comprising a luxAB construct of Ca2+, incubating the Ca2+ depleted bacteria with an agent the antibacterial effect of which shall be determined, recording the light emitted by the bacteria upon addition of an aldehyde, the incubation being carried...

20060134725 - Method for the determination of the presence of an antibiotic in a fluid: The present invention provides a method for determining the presence or absence of an antibiotic in a fluid comprising: (a) contacting a fluid sample with a test medium comprising a test microorganism and at least one indicator; (b) incubating the test microorganism with the fluid under conditions whereby growth of...

20060134726 - Modular compositing-multiple lot screening protocols for detection of pathogens, microbial contaminants and/or constituents: Particular aspects provide a method of sampling, testing and validating test lots (e.g., single-unit production lots), comprising: assembling a plurality of product portions from each of a plurality of test lots and combining the collected product portions to provide a corresponding set of test lot samples (wherein each test lot...

20060134730 - Culture medium for detecting micro-organisms: A culture medium to detect microorganisms containing carrageenan as a gelling factor, the carrageenan is preferably iota carrageenan. The culture may also comprise an agent releasing oxygen, such as nitrate oxide....

20060134728 - Microbial detection and quantification: A method for semi-quantitatively or quantitatively detecting the presence of a microbe in a sample is provided. The method utilizes a test dye that undergoes a detectable color change in the presence of one or more microbes. For example, in one embodiment, the test dye is a solvatochromic dye (e.g.,...

20060134729 - Process for the universal detection of microorganisms and reaction environment permitting the implementation of the process: A process for detecting microorganisms present in a biological fluid including a) contacting a sample of the biological fluid with a reaction environment comprising a marking agent that is a derivative of cyanines and at least one reactant of cellular penetration of the membrane of the microorganisms, b) filtering the...

20060134727 - Rapid coliform detection sytem: A system for rapidly determining the presence or quantity of coliform bacteria in a water sample using the enzymes β-D-galactosidase and β-D-glucuronidase. The system includes a first filter means for separating bacteria from the sample and a broth for culturing the bacteria including an inducing agent for inducing enzyme production....

20060134732 - Methods and compositions for a microemulsion-based tissue treatment: The invention is directed to methods and compositions for deparaffinizing paraffin-embedded biological samples for subsequent tissue staining. The compositions are microemulsions that may include water/oil/surfactant microemulsions, and optionally a cosurfactant. The microemulsions enable deparaffinization without the use of xylene or toluene, and also enable solvent exchange without the use of...

20060134731 - Stain characterization for automated cytology: A method is provided for analyzing and characterizing the formulation of a cell stain. In particular, the present invention provides a method of stain quality control using parameters derived from an imaging based automated cytology system to characterize the stain performance....

20060134733 - Production of functional proteins: balance of shear stress and gravity: The present invention provides for a method of culturing cells and inducing the expression of at least one gene in the cell culture. The method provides for contacting the cell with a transcription factor decoy oligonucleotide sequence comprising a nucleotide sequence encoding a shear stress response element....

20060134734 - Astaxanthin production using fed-batch fermentation process by phaffia rhodozyma: Disclosed is a fermentation method of astaxanthin using Phaffia rhodozyma comprising the steps of: (a) in the growing phase, feeding of a nutrient medium containing glucose or sucrose based on the specific growth rate (μ) of Phaffia rhodozyma cells, and (b) in the astaxanthin production phase, feeding of the nutrient...

20060134736 - Human growth hormone conjugated with biocompatible polymer: The present invention relates to conjugates of biocompatible polymers and hGH, particularly PEG-hGH, where the activated biocompatible polymer is conjugated to a carboxyl group of hGH at a molar ratio of 2:1 or less, preferably 1:1, methods of preparation, and related pharmaceutical compositions. The PEG-hGH conjugates have up to 20%...

20060134735 - Process for producing extract for cell-free protein synthesis and cell extract produced thereby: It is intended to provide a process for producing an extract for cell-free protein synthesis whereby the productivity of a protein and the production efficiency can be improved. Cells are cultured under suppressed growth conditions. In the stationary phase of the culture, the cells are collected and then disrupted. The...

20060134737 - Ubiquitinated tnf receptor2 and its uses: The present invention relates to ubiquitinated TNF receptor 2, and the uses thereof. More specifically, the invention relates to the use of TNF receptor 2 ubiquitination to deplete TNF receptor 2 from the cell membrane and cytoplasm, and relocalize it in the insoluble cell fraction. Such relocalization can be used...

20060134746 - Adding photoregulated amino acids to the genetic code: Compositions and methods of producing components of protein biosynthetic machinery that include orthogonal leucyl-tRNAs, orthogonal leucyl-aminoacyl-tRNA synthetases, and orthogonal pairs of leucyl-tRNAs/synthetases, which incorporate photoregulated amino acids, OMe-L-tyrosine, α-aminocaprylic acid, or o-nitrobenzyl cysteine into proteins are provided in response to an amber selector codon. Methods for identifying these orthogonal pairs...

20060134741 - Composition and methods relating to senp1-a sentrin-specific protease: The invention relates to a novel protease, called SENP1, which is active against sentrin-modified proteins in vivo. The invention more specifically relates to the genomic and amino acid sequences for SENP1, compositions related to and based on these sequences, and methods of using these sequences and compositions....

20060134740 - Engineered proteases for affinity purification and processing of fusion proteins: The present invention is directed to the identification of a protease prodomain that is capable of binding a corresponding protease with high affinity. The protease prodomain of the present invention is fused to a second protein to form a protease prodomain fusion protein. The presence of a protease prodomain protein...

20060134738 - Gene expression inducing fusion protein and method for controlling gene expression induction: The invention relates to a gene expression inducing fusion protein comprising a) a ribonucleic acid linking peptide domain and a post-transcriptional gene expression activator domain and b) a domain enabling cytoplasmic membrane delocalization. The invention also relates to a modulatable permanent external method for controlling gene expression induction by modulating...

20060134747 - Heterologous alpha amylase expression in aspergillus: The present invention relates to the co-expression and production of a heterologous alpha amylase and an endogenous glucoamylase in an Aspergillus strain and enzyme compositions including the same....

20060134752 - Human diacylglycerol acyltransferase 2 (dgat2) family members and uses therefor: The present invention relates to compositions and methods for the diagnosis and treatment of obesity and related metabolic disorders. The invention provides isolated nucleic acids molecules, designated DGAT2 family member nucleic acid molecules, which encode diacylglycerol acyltransferase family members. The invention also provides recombinant expression vectors containing DGAT2 family member...

20060134751 - Identification of novel ms4a gene family members expressed by hematopoietic cells: Isolated nucleic acids encoding MS4A polypeptides, isolated MS4A polypeptides, and uses thereof. The disclosed MS4A nucleic acids and polypeptides can be used to generate a mouse model of atopic disorders, for drug discovery screens, and for therapeutic treatment of atopic disorders or other MS4A-related conditions....

20060134744 - Method and apparatus for the production of soluble mhc antigens and uses thereof: The field of the invention relates in general to at least one method and apparatus for the production of soluble MHC antigens and more particularly, but not by way of limitation, to at least one method and apparatus for the production of soluble Class I and II HLA molecules. The...

20060134750 - Method of treatment using interferon-tau: Methods of treating an autoimmune condition by administering IFNτ are described. IFNτ is administered orally at a dose sufficient to achieve obtain a desired clinical endpoint, such as a reduction in new contrast-enhanced brain lesions in multiple sclerosis patients....

20060134745 - Nucleic acid sequences encoding two-component sensing and regulatory proteins, antimicrobial proteins and uses therefor: Stress-related nucleic acid molecules and polypeptides and fragments and variants thereof are disclosed in the current invention. In addition, stress-related fusion proteins, antigenic peptides, and anti-stress-related antibodies are encompassed. The invention also provides recombinant expression vectors containing a nucleic acid molecule of the invention and cells into which the expression...

20060134748 - Orthogonal suppressor trnas and aminoacyl-trna synthetases and uses thereof: The present invention provides novel orthogonal suppressor tRNAs, aminoacyl-tRNA synthetases, and suppressor tRNA/aminoacyl-tRNA synthetase pairs. In preferred embodiments the suppressor tRNAs function in mammalian cells and in certain embodiments they are not aminoacylated by any of the mammalian cytoplasmic aminoacyl-tRNA synthetases. The invention provides a novel ochre suppressor tRNA that...

20060134742 - Production of ggl with low environmental impact: A method is disclosed for increasing the specific activity of myo-inositol oxygenase. The method includes incubating a mixture including myo-inositol oxygenase and a non-sulfur containing reductant under conditions effective to increase the specific activity of the myo-inositol oxygenase. Also disclosed are methods for producing D-glucuronic acid and glucurono-γ-lactone comprising incubating...

20060134749 - Protease, dna encoding the same, and method for manufacturing protease: The present invention provides a protease, DNA encoding this protease, a recombinant vector comprising this DNA, and a method for manufacturing the protease using the transformant....

20060134739 - Synthesis of proteins by cell-free protein expression: In Vitro peptide/protein or biological macromolecule synthesis systems, and methods and kits thereof improve efficiency of in vitro protein or biological macromolecule synthesis and related compositions....

20060134743 - Vectors and methods for enhanced cell longevity and protein expression: It is the object of the current invention to provide methods and compositions relating to the expression of vankyrin proteins in cell lines to increase their viability, longevity and capacity for protein production. The inventors have discovered that the expression of P-ank-1 and I2-ank-3 proteins in cell culture has increased...

20060134753 - Super-antigen fusion proteins and the use thereof: The present invention relates to a super-antigen fusion protein, comprising: a peptide fragment encoding a partial SARS E2 spike protein; and a translocating peptide fragment for transporting a protein into a cell and translocating the protein in cytosol; wherein, the amino acid of the peptide fragment encoding the partial SARS...

20060134755 - Il-17 homologous polypeptides and therapeutic uses thereof: The present invention is directed to novel polypeptides and to nucleic acid molecules encoding those polypeptides. Also provided herein are vectors and host cells comprising those nucleic acid sequences, chimeric polypeptide molecules comprising the polypeptides of the present invention fused to heterologous polypeptide sequences, antibodies which bind to the polypeptides...

20060134754 - Il-21 production in prokaryotic hosts: The expression vectors and methods using an E. coli expression system for the large scale production of IL-21 are described. The vectors utilize the IL-21 coding sequence with specific changes in nucleotides in order to optimize codons and mRNA secondary structure for translation in E. coli. Using the expression vectors,...

20060134756 - Anti-il-20 antibodies and binding partners and methods of using in inflammation: The present invention relates to blocking, inhibiting, reducing, antagonizing or neutralizing the activity of IL-20 polypeptide molecules. IL-20 and IL-22 are cytokines that are involved in inflammatory processes and human disease. The present invention includes anti-IL-20 and anti-IL-22RA antibodies and binding partners, as well as methods for antagonizing IL-20 using...

20060134757 - Microorganism and the process for the production of teicoplanin: The present invention provides a newly isolated mutant culture of Actinoplanes teichomyceticus BNG 2315. This culture is capable of producing teicoplanin more than 60 times productivity than those reported before (e.g., Actinoplanes teichomyceticus nov. sp. ATCC 31121). The present invention also provides a fermentation process for the production of teicoplanin...

20060134759 - Isolation of glucan particles and uses thereof: The present invention relates to the isolation of novel glucan particles but also to mannoprotein from natural sources such as yeast cell walls, novel isolation methods, and the use of products thereof....

20060134758 - Process for obtaining a ccellulosic wet sheet and a membrane, the equipment used to obtain the membrane and the membrane obtained: Said process used to obtain, in industrial scale, wet sheets and membranes of bacterial cellulose of high purity and specific physical and chemical properties, using the appropriate culture medium that is prepared in a mechanical mixer furnished with a jacket for temperature control and heat exchange, using selected recycled or...

20060134760 - Process for the production of l-amino acids using strains of the family enterobacteriaceae that contain an attenuated frur gene: A process for the production of L-amino acids, in particular L-threonine, in which the following steps are carried out: (a) fermentation of the microorganisms of the family Enterobacteriaceae producing the desired L-amino acid, in which the fruR gene or nucleotide sequences coding therefor are attenuated, in particular are switched off,...

20060134761 - Nucleotide sequences coding for the luxr gene: The present invention relates to polynucleotides corresponding to the luxR gene and which encode a LuxR transcriptional activator, methods of producing L-amino acids, and methods of screening for polynucleotides which encode proteins having LuxR transcriptional activation activity....

20060134762 - Novel endophytic camptothecin and camptothecinoid producing fungi and process of producing the same: The present invention relates to a novel source of microorganism for production of Camptothecin and related camptothecinoids. The invention also discloses its isolation, screening for Camptothecin production, growth, fermentation requirements and chemical analysis of Camptothecin (camptothecinoids)....

20060134763 - Stereoselective process for the production of dioxolane nucleoside analogues: The present invention relates to a process for producing a compound of formula (I); said process comprising the steps of: a) subjecting a compound of formula (II) to an enzymatic diastereomeric resolution in the presence of a suitable amount of enzyme chosen from Pig Liver Esterase or Porcine Pancreatic Lipase...

20060134764 - Gene cluster involved in safracin biosynthesis and its uses for genetic engineering: A gene cluster has open reading frames which encode polypeptides sufficient to direct the synthesis of a safracin molecule....

20060134765 - Fermentative production of mannitol: The invention provides processes for continuous fed-batch fermentative production and continuous recycle fermentative production of mannitol....

20060134766 - Enhancement of enzyme activity through purification and immobilization: An improved method of making an immobilized enzyme comprising (a) treating an immobilization support with an aqueous solution comprising a cross-linking agent and polymeric aldehyde species and active centre species to produce a modified support; (b) isolating the modified support; (c) treating an enzyme solution with the modified support to...

20060134768 - Erbb4 co-crystal: A crystal structure of the ErbB4 kinase domain (ErbB4K), specifically the ErbB4K in liganded form as well as methods of using the same in the discovery of ErbB4 inhibitors. Disclosed further are methods of treatment of diseases mediated by inappropriate ErbB4 activity utilizing ErbB4 inhibitors identified by the methods disclosed...

20060134767 - Mitotic kinesin binding site: The present invention is directed to the identification, characterization and three-dimensional structure of a novel ligand binding site of KSP. Binding of ligands to the novel binding site result in a conformational change in the three-dimensional structure of the protein and a modulation of the activity of KSP. This conformational...

20060134769 - Process for producing a virus-inactivated thrombin preparation: The present invention relates to processes for the preparation of virus inactivated thrombin, and to the products prepared by these processes....

20060134770 - Microorganisms for assimilating oils or fats, etc. and methods for treating waste liquids and deodorizing by using the same: The microorganisms for assimilating oils, fats or an organic waste material, etc. according to the present invention can efficiently decompose waste oils, fats or organic waste materials, etc. contained in a waste liquid and reduce concentrations of such waste oils, fats or organic waste materials, etc. to a level lower...

20060134771 - Biofiltration apparatus and method for reducing methyl bromide concentration in a volume of gas: A biofiltration apparatus and method for reducing methyl bromide concentration in a volume of a gas are disclosed. The apparatus includes a housing and a substrate component located in the housing. The substrate component includes a population of methyl bromide degrading microorganisms. The housing receives a volume of contaminated gas,...

20060134772 - System for locating cells and for cellular analysis: A system for locating a cell for analysis. A capture structure is provided with a flow channel in the capture structure and a capture well in the flow channel. A fluid flows along the fluid flow channel and through the capture well. The cell flows with the fluid along the...

20060134773 - Device for pretreating specimen: Due to the recent decipherment of the human genome, various relations between life processes and genes have been analyzed. Consequently, medical importance shifts from pathology to etiology, and from medical treatment to prevention. Hereupon, the gene test technology becomes an important basis. A pretreatment device for pretreating a specimen comprises...

20060134774 - Detection of protease enzymes: Methods and compositions for the detection of biomolecules, for example, proteases, are provided. The novel compositions, methods, and kits of the present invention have broad applicability in the detection of proteases, and providing enhanced specificity in the detection of proteases. The compositions and methods may be used to measure the...

20060134775 - Systems, illumination subsystems, and methods for increasing fluorescence emitted by a fluorophore: Systems, illumination subsystems, and methods for increasing fluorescence emitted by a fluorophore are provided. One system configured to measure fluorescence of particles includes an illumination subsystem configured to illuminate the particles with light having linear polarization oriented in a non-vertical direction, circular polarization, or elliptical polarization. The polarization of the...

20060134776 - Device for cereal malting: The invention relates to a device (1) for malting cereals comprising a tower (2) consisting a certain number of stories which are separated from each other by storey floors (9, 10), each storey comprising an air-proof carrier plate (13) carrying germinating cereals (24). Said tower also comprises air conditioning means...

20060134777 - Microfluidic device comprising electrolysis device for cell lysis and method for electrochemically lysing cells using the same: Provided are a microfluidic device including an electrolysis device for cell lysis which includes an anode chamber, a cathode chamber and a separator, in which the separator is installed between the anode chamber and the cathode chamber, the anode chamber includes an inlet and an outlet for an anode chamber...

20060134778 - Method of treating a malignancy in a subject via direct picornaviral-mediated oncolysis: There are provided methods for treatment of abnormal cells such as cancer cells in a mammal. The methods involve treating the mammal with virus selected from echoviruses and modified forms and combination thereof, which recognise α2β1 for infectivity of the cells. There are also provided methods for screening viruses for...

20060134779 - Modulation of cell intrinsic strain to control cell modulus, matrix synthesis, secretion, organization, material properties and remodeling of tissue engineered constructs: The present invention provides methods for manipulating the intrinsic strain of cells by treating tissue engineered constructs or native tissue with compounds which affect the intrinsic strain setpoint of the cells in order to modulate matrix synthesis, secretion, organization and/or remodeling so that the tissues withstand in vivo mechanical forces...

20060134782 - Differentiated cells suitable for human therapy: This invention provides a system for producing differentiated cells from a stem cell population for use wherever a relatively homogenous cell population is desirable. The cells contain an effector gene under control of a transcriptional control element (such as the TERT promoter) that causes the gene to be expressed in...

20060134781 - Three-dimensional cell culture system: A three-dimensional culture system provides an efficient mechanism for producing stems cells derived from adipose tissue. A three-dimensional matrix is formed in the presence of adipose tissue to incorporate the adipose tissue into the three-dimensional matrix. After incubation, the 3-D matrix may be degraded to liberate stem cells, or progeny...

20060134780 - Transgenic animals for producing specific isotypes of human antibodies via non-cognate switch regions: The present invention provides fully human antibodies in a transgenic animal of a desired isotype in response to immunization with any virtually any desired antigen. The human immunoglobulin heavy chain transgene in the foregoing animals comprises a human constant region gene segment comprising exons encoding the desired heavy chain isotype,...

20060134784 - Methods and compositions for the growth and maintenance of stem cells: The present invention relates to pluripotent stem cells and methods of promoting long term growth of such cells. In particular, the methods described are used to enhance the proliferation of hematopoietic stem cells, hematopoietic precursor cells, hemangioblasts and endothelial stem cells. The present invention also relates to methods of using...

20060134783 - Methods of expanding myeloid cell populations and uses thereof: The present disclosure relates to a method of expanding myeloid progenitor cells by culturing an initial population of cells in a medium comprising a mixture of cytokines and growth factors that promote growth and expansion of the myeloid progenitor cells. The expanded cell population provides a source of cells as...

20060134785 - Nanoparticles for the administration of active ingredients, method of producing said particles and composition containing same: The invention relates to nanoparticles used for the administration of active ingredients, the method of producing said nanoparticles and compositions containing same. The inventive nanoparticles comprise a chitosan, glucomannan, at least one active ingredient and, if necessary, an anionic salt, preferably in sodium tripolyphosphate form. Said nanoparticles can be used...

20060134786 - Nucleotide sequences and polypeptides encoded thereby useful for modifying plant characteristics: Isolated polynucleotides and polypeptides encoded thereby are described, together with the use of those products for making transgenic plants....

20060134787 - Methods and compositions for enhancing the efficacy and specificity of single and double blunt-ended sirna: The present invention provides methods of enhancing the efficacy and specificity of RNAi using single or double blunt-ended siRNA. The invention also provides single and double-blunt ended siRNA compositions, vectors, and transgenes containing the same for mediating silencing of a target gene. Therapeutic methods are also featured....

20060134789 - Methods and products for biasing cellular development: Methods are described that bias cells, such as potent and multipotent stem cells, by transfection with a nucleic acid sequence, to differentiate to a desired end-stage cell or a cell having characteristics of a desired end-stage cell. In particular embodiments, human neural stem cells are transfected with vectors comprising genes...

20060134788 - Recombinant vector containing mj1 gene and method of site-specific integration using the same: The present invention relates to a recombinant vector comprising MJ1 gene coding an integrase derived from Enterococcus temperate bacteriophage φ FC1 and an integration method using the vector. More particularly, the present invention relates to a recombinant vector comprising MJ1 gene which can make a site-specific integration in the human...

20060134790 - Solid surface with immobilized degradable cationic polymer for transfecting eukaryotic cells: A cell transfection/culture device is disclosed which includes a solid support coated with a degradable polymer cation as a transfection reagent. The transfection/culture device is conveniently stored at room temperature until use. Cell transfection is accomplished easily by adding the nucleic acid of interest and the cells to be transfected...

20060134791 - Compositions and methods for activating genes of interest: Compositions and methods for activating genes of interest are provided. The compositions comprise a masked targeted expression cassette which expresses a gene product only in the presence of a target molecule. The cassettes are useful for the treatment of disease and for preventing the proliferation of neoplastic cells....

20060127873 - Composition for cytocompatible, injectable, self-gelling chitosan solutions for encapsulating and delivering live cells or biologically active factors: The present invention provides compositions and methods for tissue repair using a cytocompatible self-gelling cross-linked hydrogel. The composition comprises a biocompatible mixture of chitosan, bifunctional dialdehyde, and hydroxylated polymer, which can be used to immobilize or encapsulate viable cells, or bioactive substances. The method includes the process of mixing bioactive...

20060127875 - Device for separation of platelets from whole blood: A method is described for separating, retrieving and concentrating platelets from whole blood relying on aggregation of the platelets followed by filtration. This method eliminates the need of a centrifuge for separating said cells from blood. To obtain cellular concentrates of platelets, blood is mixed with compatible agents that will...

20060127876 - Method to treat collagenous connective tissue for implant remodeled by host cells into living tissue: The invention relates to a method of treatment of collagenous connective tissue removed from a donor for implant into a recipient which is re-habited or re-colonized by host cells without an immune rejection and inflammatory reaction. After removal from the donor the tissue is trimmed and thereafter soaked in a...

20060127874 - Plasma preparation or serum preparation and process for producing the same: The present invention relates to a plasma product or a serum product with an extremely low risk of viral contamination and a method for producing the same. Before treating plasma or serum to be used as a raw material for producing a plasma product or a serum product using a...

20060127881 - Automated segmentation, classification, and tracking of cell nuclei in time-lapse microscopy: Methods and apparatus are provided for the automated analysis of images of living cells acquired by time-lapse microscopy. The new methods and apparatus can be used for the segmentation, classification and tracking of individual cells in a cell population, and for the extraction of biologically significant features from the cell...

20060127882 - Circuit and method to non-invasively detect the electrical potential of a cell or neuron: A system and method for non-invasively measuring the electrical potential radiated by a cell. To do this, a probe is positioned within ten microns distance from the cell for receiving the signal. Also, a reference potential is determined for the cell's environment. A sensor records the signal and compares the...

20060127880 - Computerized image capture of structures of interest within a tissue sample: A computerized method of automatically capturing an image of a structure of interest in a tissue sample. The computer memory receives a first pixel data set representing an image of the tissue sample at a low resolution and an identification of a tissue type of the tissue sample, and selecting...

20060127879 - Electrodynamic profiling of genomic response in the cell: A method of cellular evaluation based on the electronic nature of cells is reveled though cellular reproductions use of a magnetic force. The dynamic process of nuclear response is shown to be electronic in nature relative to DNA mediating electrons hydrogen bonding in bases pairing of DNA though out the...

20060127877 - Fluorescent protein: m

20060127878 - Hydrogel preparation and process of manufacture thereof: A polymeric hydrogel having a network of a macropores and micropores formed by copolymerizing at least one monomer having at least one double bond and at least one crosslinker having at least two double bonds in the presence of an organic additive forming a hydro-organic system with water, and uses...

20060127883 - Spatially directed ejection of cells from a carrier fluid: This invention is directed to the use of focused energy, particularly focused acoustic energy, in the spatially directed ejection of cells suspended in a carrier fluid, e.g., for providing a pattern of cells on a substrate surface, such as a cellular array....

20060127884 - Toll-like receptor 9 modulators: TLR9 is localized to endoplasmic reticulum and upon stimulation with a TLR9 ligand, is transported to a tubular lysosomal compartment as is CpG-DNA. Furthermore, it is shown that TLR9 and CpG-DNA directly bind. It was also found that the MyD88 translocates in response to activation of TLR9-mediated signaling. Methods of...

20060127888 - Compositions and methods for enhancing the identification of prion protein prpsc: The present invention provides compositions, methods and kits for enhancing the amplification of PrPSc for use in increasing the sensitivity of identifying the presence of PrPSc in a sample....

20060127887 - Isolation and purification method of biomolecules using hydrogel: Provided is a method of isolating and purifying biomolecules using a hydrogel, the method including: bring a sample containing charged biomolecules into contact with a hydrogel to bind the biomolecules to the hydrogel; washing the hydrogel bound with the biomolecules; and eluting the bound biomolecules using an elution solvent. According...

20060127885 - Method and devices for rapid diagnosis of foot-and-mouth disease: A rapid immunoassay method and apparatus for detecting foot and mouth disease virus are disclosed. The method and test device permit pen-side testing of animals and provide test results within a relatively short time period. In a preferred embodiment, the method and apparatus provide a means for differentiating between FMDV-infected...

20060127886 - Sample-efficient lateral flow immunoassay: There is provided a lateral flow assay device for detecting the presence or quantity of an analyte residing in a test sample where the lateral flow assay device has a porous membrane in communication with a conjugate pad and a wicking pad. The porous membrane has a detection zone which...

20060127899 - Active carbohydrate containing protecting reagents for chemical modifications, their production and use: The present invention relates to new active carbohydrate containing protecting reagents represented by a carbohydrate central unit, which is attached to a polymer chain at least at one of the hydroxyl groups and to an active linkage group at the anomeric position of the carbohydrate central unit. These new compounds...

20060127913 - Alternatively spliced isoforms of human phka2: The present invention features nucleic acids and polypeptides encoding four novel splice variant isoforms of PHKA2. The polynucleotide sequences of PHKA2sv3, PHKA2sv4, PHKA2sv6 and PHKA2sv7 are provided by SEQ ID NO 1, SEQ ID NO 3, SEQ ID NO 5 and SEQ ID NO 6, respectively. The amino acid sequences...

20060127910 - Bpl1 as an antifungal target: The invention provides biotin protein ligase 1 (BPL1) as a novel antifungal target, screening methods for BPL1 inhibitors and their use as antifungal compounds, pharmaceutical compositions containing them and their use in medicine specifically in the treatment of an individual susceptible to or suffering from an anti-fungal infection. In particular...

20060127902 - Brain endothelial cell expression patterns: To gain a better understanding of brain tumor angiogenesis, new techniques for isolating brain endothelial cells (ECs) and evaluating gene expression patterns were developed. When transcripts from brain ECs derived from normal and malignant colorectal tissues were compared with transcripts from non-endothelial cells, genes predominantly expressed in the endothelium were...

20060127908 - Cancer associated plexinb1 mutations: The present inventors have identified cancer associated mutations in plexinB1, which is a transmembrane receptor that mediates semaphorin signaling. The frequency of plexinB1 mutations, in particular in prostate and breast cancer, indicates that plexinB1 may be useful in the screening and diagnosis of cancer and as a drug target in...

20060127911 - Compositions and methods for enhancing the identification of prion protein prpsc: The present invention provides compositions, methods and kits for enhancing the amplification of PrPSc for use in increasing the sensitivity of identifying the presence of PrPSc in a sample....

20060127936 - Compositions and methods for labeling of nucleic acid molecules: The present invention is generally related to compositions, kits and methods for labeling nucleic acid molecules using reverse transcriptases, preferably multi-subunit reverse transcriptases such as ASLV reverse transcriptases. Specifically, the invention relates to methods, kits and compositions for fluorescently labeling nucleic acid molecules during nucleic acid synthesis. The labeled nucleic...

20060127919 - Compositions and methods relating to cell adhesion molecule l1: A method of identifying a compound that induces apoptosis in a cell is disclosed. The method includes contacting the cell with a putative apoptosis-inducing compound and determining whether the compound inhibits L1. Also disclosed are methods for inducing apoptosis in a cell by inhibiting L1. The invention further includes methods...

20060127917 - Comprehensive virus disinfecting strategy and methods for making a preventive anti-virus medical product: The present invention provides a comprehensive virus disinfecting strategy with more than two biologically active anti-virus components work synergistically to form a multi-hurdle defending line for virus disinfecting, and methods for making a medical product with anti-virus properties. The comprehensive preventive system for virus disinfecting, combines components competitively inhibiting virus...

20060127916 - Concurrent optimization in selection of primer and capture probe sets for nucleic acid analysis: Disclosed is a method of iteratively optimizing two (or more) interrelated sets of probes for the multi-step analysis of sets of designated sequences, each such sequence requiring, for conversion, at least one conversion probe (“primer”), and each converted sequence requiring, for detection, at least one capture probe. The iterative method...

20060127897 - Delete sequence in m, tuberculosis, method for detecting mycobacteria using these sequences and vaccines: The present invention is the identification of a nucleotide sequence which make it possible in particular to distinguish an infection resulting from the vast majority of Mycobacterium tuberculosis strains from an infection resulting from Mycobacterium africanum, Mycobacterium canetti, Mycobacterium microti, Mycobacterium bovis, Mycobacterium bovis BCG. The subject of the present...

20060127906 - Detection system: A method for detecting the presence of a target nucleic acid sequence in a sample, said method comprising: (a) adding to a sample suspected of containing said target nucleic acid sequence, a fluorescently labelled probe specific for said target sequence, and DNA duplex binding agent which can absorb fluorescent energy...

20060127939 - Device and method for multiple analyte detection: The invention is directed to a method and device for simultaneously testing a sample for the presence, absence, and/or amounts of one or more of a plurality of selected analytes. The invention includes, in one aspect, a device for detecting or quantitating a plurality of different analytes in a liquid...

20060127940 - Dual resonance energy transfer nucleic acid probes: Dual nucleic acid probes with resonance energy transfer moieties are provided. In particular, fluorescent or luminescent resonance energy transfer moieties are provided on hairpin stem-loop molecular beacon probes that hybridize sufficiently near each other on a subject nucleic acid, e.g. mRNA, to generate an observable interaction. The invention also provides...

20060127915 - Gene mutation associated with age-related macular degeneration: The present invention describes the identification of a mutation in a human FIBL-6 protein, which mutation is associated with Familial Age-Related Macular Degeneration. Transcripts and products of this mutated gene are useful in detecting and diagnosing AMD, developing therapeutics for treatment of AMD, as well as the isolation and manufacture...

20060127898 - Holographic sensor based on a volume hologram in a porous medium: A sensor for the detection of an analyte, comprising a holographic element comprising a medium and a hologram disposed throughout the volume of the medium, wherein an optical characteristic of the hologram changes as a result of a variation of a physical property occurring throughout the volume of the medium,...

20060127904 - Hybridization sensing part, sensor chip, and hybridization method: The present invention provides a hybridization detector (1a) and the like that are improved in the hybridization efficiency by arraying nucleotide probes in a stretched form, a sensor chip including the hybridization detector (1a) or the like, and a method of hybridization using these detectors or the chip. The hybridization...

20060127935 - Identification and use of prognostic and predictive markers in cancer treatment: The present invention provides a method of screening for markers useful in predicting the efficacy of a specified cancer that includes: (a) constructing a tissue microarray from a tissue bank comprising multiple tissue samples that are annotated with clinical follow up data; (b) labeling polynucleic acid probes specific for oncogenes...

20060127938 - Immunoassay for hiv protease inhibitors: A non-isotopic immunoassay for an HIV protease inhibitor comprising incubating a sample containing the inhibitor with a receptor specific for the inhibitor or for a metabolite of said inhibitor and further with a conjugate comprising an analog of the inhibitor and a non-isotopic signal generating moiety. Signal generated as a...

20060127923 - Internal ribosomal entry site mediated gene expression: The present invention relates to an IRES element that is operably linked to one or more coding sequences, wherein the IRES element expresses said coding sequences in an endothelial cell....

20060127927 - Mammalian iap gene family, primers, probes, and detection methods: Disclosed is substantially pure DNA encoding mammalian IAP polypeptides; substantially pure polypeptides; and methods of using such DNA to express the IAP polypeptides in cells and animals to inhibit apoptosis. Also disclosed are conserved regions characteristic of the IAP family and primers and probes for the identification and isolation of...

20060127903 - Marker for cancer: The present invention relates to a cell-free immunoreactive Integrin Linked Kinase which is present in samples of biological fluids from patients suffering from ovarian cancer. The invention also relates to methods of diagnosis, screening, monitoring treatment and treatment of cancers, especially ovarian cancer, and to kits and compounds for use...

20060127896 - Materials and methods for treating cancer: The invention is concerned with the detection and treatment of nasopharyngeal carcinoma (NPC) based on differential gene expression in these cells. Specifically, the invention provides details of differentially expressed genes in NPC which serve to detect the presence or risk of the disease and its clinical type. The invention also...

20060127889 - Method for assessing transgene expression and copy number: The invention relates to the field of genetic engineering. More particularly the invention relates to a method of quantitating transgenes or transgene expression by using sequences commonly included in transformation plasmids or vectors. The invention also provides primers and probes which can be used with this method....

20060127900 - Method for detecting increased susceptibility to tumors: The invention relates to a method for detecting increased susceptibility to tumours by specifically detecting a polymorphism in the position 354 A ? G in the exon 12 of the human murine double minute-2 (MDM2) gene. Said polymorphism represents a hereditary marker for increased risk of cancer in humans. The...

20060127912 - Method for diagnosis and/or prognosis of a septic syndrome: The present invention relates to a method for diagnosing and/or predicting a septic syndrome, wherein: a. a biological sample from the patient is made available and biological material is extracted from the biological sample b. at least four specific reagents which are selected from the following specific reagents are made...

20060127944 - Method for pheromone discovery in insects: The present invention is directed generally to a method of identifying an insect pheromone. Initially, a candidate insect pheromone-binding protein is obtained and sequenced. Specific proteins may then be selected by observing the pattern of pheromone-binding protein expression in the insect stage, phase or caste; and/or in the antenna and...

20060127924 - Method for rapid identification of microorganisms: The present invention relates, in general, to probes, methods, and kits used to determine the presence or absence of a microorganism in a sample. The probes, methods, and kits comprise at least one capture probe and/or at least one detector probe....

20060127932 - Method for the snp analysis on biochips having oligonucleotide areas: The invention relates to a method for the multiparallel detection of nucleotide polymorphisms on a polydimensional array. The invention also relates to a method for detecting many individual nucleotide polymorphisms, during which the nucleotide polymorphisms of multiple individuals can be multiparallelly detected on the array. According to the invention, the...

20060127907 - Method of detecting gene mutation: DNA amplification and hybridization are successively carried out in a reaction system containing primers for the DNA amplification and hybridization probes, followed by detecting the hybrid in the reaction solution by affinity chromatography, wherein at least one of the primers to be used in the DNA amplification is labeled with...

20060127926 - Method of error reduction in nucleic acid populations: A method is disclosed for the direct synthesis of double stranded DNA molecules of a variety of sizes and with any desired sequence. The DNA molecule to be synthesis is logically broken up into smaller overlapping DNA segments. A maskless microarray synthesizer is used to make a DNA microarray on...

20060127933 - Methods and compositions for treating & diagnosing mood disorders, schizophrenia, and neuro-psychiatric disorders: This invention provides: methods of determining a predisposition or susceptibility of a subject to a mood disorder, a schizophrenia, or a neuro-psychiatric disease or disorder, comprising detecting a presence of a polymorphism in a vesicular monoamine transporter 1 (VMAT1) gene or a haplotype comprising the polymorphism, and methods of treating...

20060127922 - Methods for detecting, grading or monitoring an h. pylori infection: The present invention provides methods for determining the status of an H. pylori infection by detecting a high-integrity H. pylori nucleic acid in a patient sample....

20060127930 - Methods of attaching biological compounds to solid supports using triazine: Disclosed are methods of attaching biologically active compounds to a solid surface, comprising modifying the solid surface using triazine chloride and attaching the biologically active compound to the triazine moiety....

20060127893 - Modification of human variable domains: The present invention relates to a method for the optimization of isolated human immunoglobulin variable heavy (VH) and light (VL) constructs....

20060127914 - Nicotinic acetylcholine receptor: The invention provides nucleic acid encoding human alpha nicotinic acetylcholine receptor (α10AChR), isolated α10AChR, and assay methods utilising the same....

20060127890 - Novel gene overexpressed in heart and skeletal muscle and use thereof: It is an object of the present invention to provide a novel protein and a DNA encoding the same. A compound or a salt thereof controlling an activity of the protein, and a neutralizing antibody controlling the activity of the protein are useful as, for example, a preventive and therapeutic...

20060127937 - Novel survivin interacting protein tpr1: Survivin Interacting Protein 1 and polynucleotides and methods for producing such polypeptides by recombinant techniques are disclosed. Also disclosed are methods for ulilizing TPR1 polypeptides and polynucleotides in diagnostic assays....

20060127941 - Nuclear matrix protein fluid assay: Disclosed is a method for detecting and quantitating soluble nuclear matrix proteins in body fluids and extracellular media. The method is useful for monitoring the viability of cells and tissue, for evaluating the progress of a disease or its treatment, and for evaluating the cytotoxicity of unknown compounds. Also disclosed...

20060127918 - Nucleic acid arrays: Described is a nucleic acid-containing substrate that is useful for nucleic acid hybridization methods, such as methods that utilize nucleic acid microarrays. The substrate contains nucleic acid that had not been covalently modified prior to having been contacted with the substrate and/or nucleic acid that is bound substantially non-covalently to...

20060127934 - Oligonucleotides useful in methods for detecting and characterizing aspergillus fumigatus: Oligonucleotides and methods for using these oligonucleotides in the detection of Aspergillus fumigatus are disclosed. Aspergillus fumigatus is the causative agent for medical conditions including invasive aspergillosis. The oligonucleotides of the invention have nucleotide sequences derived from the gene encoding the cytochrome P450 14 alpha-sterol demethylase (i.e., the Cyp51A protein)...

20060127942 - Particle analysis assay for biomolecular quantification: A method is provided for carrying out multi-step separations of objects bearing at least two binding sites. In the first step, a first binder/bead composition is bound to objects that bear the first binding site, and then unbound objects, i.e. objects not bearing the first binding site, are separated from...

20060127931 - Particle detector with waveguide light confinement: A strong light confining nano-cavity in a photonic structure enhances the effective extinction cross-section of metal nano-particles. As a result of strong light confinement, precisely where the particle is located, the presence of a single metal nano-particle with a diameter as small, or smaller than 10 nm may be detected...

20060127921 - Pecam-1-related molecules compositions kit of parts and associated methods of use: An isolated polynucleotide coding for platelet endothelial cell adhesion molecule-1 (PECAM-1), and obtainable by amplifying cDNA from total human white blood cells by PCR; peptides encoding PECAM-1 5th Ig-like domain; an antibody against one of the peptides; associated compositions methods and kits of parts....

20060127929 - Polymers for analyte detection: The present invention generally relates to organic polymers able to participate in an analyte-recognition process, where an analyte facilitates an energy transfer between an energy donor and an energy acceptor. Certain embodiments of the invention make use of fluorescent conjugated polymers, such as poly(phenylene ethynylene)s and other polymers comprising pi-conjugated...

20060127920 - Polynucleotide synthesis: Methods of improving the kinetics of bimolecular interactions where reactants are present in low concentrations are provided. Methods of pre-amplifying one or more oligonucleotides using high concentration universal primers are provided. Methods of improving the error rate in oligonucleotide and/or polynucleotide syntheses are also provided. Methods for sequence optimization and...

20060127909 - Polynucleotides targeted against hter and use thereof: The present invention relates to polynucleotides directed towards a gene of a catalytic subunit of human telomerase, as well as to the use of said polynucleotides for the diagnosis, prophylaxis, reduction and follow-up of diseases associated with cell growth, differentiation and/or division, such as tumor diseases....

20060127894 - Protein associated with cell growth, differentiation, and death: Various embodiments of the invention provide human proteins associated with cell growth, differentiation, and death (CGDD) and polynucleotides which identify and encode CGDD. Embodiments of the invention also provide expression vectors, host cells, antibodies, agonists, and antagonists. Other embodiments provide methods for diagnosing, treating, or preventing disorders associated with aberrant...

20060127892 - Quantitative diagnostic analysis of hypertonia: The invention relates to the application of the direct correlation between the overexpression or the functional molecular modification of human homologs of the sgk family and hypertension for quantitative diagnosis of a particular form of genetically determined hypertension. In particular the invention relates to the detection of a direct link...

20060127891 - Rna interference mediated inhibition of map kinase gene expression or expression of genes involved in map kinase pathway using short interfering nucleic acid (sina): The present invention concerns methods and reagents useful in modulating MAP kinase gene expression in a variety of applications, including use in therapeutic, diagnostic, target validation, and genomic discovery applications. Specifically, the invention relates to small nucleic acid molecules, such as short interfering nucleic acid (siNA), short interfering RNA (siRNA),...

20060127905 - Rna-exit-channel: target and method for inhibition of bacterial rna polymerase: The invention provides a target and methods for specific binding and inhibition of RNAP from bacterial species. The invention is directed to a method for identifying agents that bind to a bacterial RNAP homologous RNA-exit-channel amino-acid sequence, comprising preparing a reaction solution comprising the agent to be tested and an...

20060127943 - Scheduling system and scheduling method for the same: A schedule management table is additionally provided in a scheduling system comprising: a time slot for specifying, in slots, the cell transfer sequence of individual lines in one turn; a cell read sequence management table having elements to which the lines are set as cell transfer objects; schedule computation means...

20060127901 - Sim2 polypeptides and polynucleotides and uses of each in diagnosis and treatment of ovarian, breast and lung cancers: A method of diagnosing predisposition to, or presence of ovarian cancer, breast cancer and/or lung cancer in a subject is provided. The method comprises determining a level of SIM2 in a lung tissue, breast tissue and/or ovarian tissue of the subject, the level being correlatable with predisposition to, or presence...

20060127925 - Stimuli-responsive polymer conjugates and related methods: Conjugates that include polymers that are reversibly self-associative in response to a stimulus and methods for using the conjugates....

20060127928 - Targeted therapy marker panels: The present invention relates to a panel of targeted therapy markers that can be used in assessing a particular subject's sensitivity to various therapeutic agents and cancer treatments as a means of prognosticating whether a treatment or use of a particular therapeutic agent will result in a clinically positive outcome....

20060127895 - Use of c-jun or c-jun activating agents such as uv or c-jun n-terminal kinases (junks) for treating cancer: The invention concerns the increased stabilization and activation of p73, a homologue of p53. Specifically, the inventors have determined that c-Jun, a component of the AP-1 family of transcription factors, is necessary for stabilization and activation of p73. Thus, there is provided a mechanism for initiating an apoptotic pathway involving...

20060127948 - Analysis of proteins from biological fluids using mass spectrometric immunoassay: Presented herein are methods, devices and kits for the mass spectrometric immunoassay (MSIA) of proteins present in complex biological fluids or extracts. Pipettor tips containing porous solid supports that are covalently derivatized with affinity ligand and used to extract specific proteins and their variants from various biological fluids. Nonspecifically bound...

20060127949 - Device for detecting molecules, method for detecting molecules: A construct is provided that is capable of binding a plurality of molecules, the construct comprising a first moiety with a first molecule binding region and a first molecule non-binding region; and a second moiety with a second molecule-binding region and a second molecule-non-binding region, whereby the first binding region...

20060127945 - Method for determining endoglycosidase enzyme activity: The invention relates to a method for determining endoglycosidase enzyme activity, and in particular of the heparanase type, in a sample, and also to a method for detecting a compound capable of modulating the activity of an endoglycosidase, and in particular of an endoglycosidase having activity of the heparanase type,...

20060127950 - Methods and products related to the improved analysis of carbohydrates: The invention relates, in part, to the improved analysis of carbohydrates. In particular, the invention relates to the analysis of carbohydrates, such as N-glycans and O-glycans found on proteins and saccharides attached to lipids. Improved methods, therefore, for the study of glycosylation patterns on cells, tissue and body fluids are...

20060127952 - Rapid diagnostic methods of peste des petits ruminants using recombinant nucleocapsid protein expressed in insect cells and monoclonal antibody: The present invention relates to a diagnostic method of Peste des Petits Ruminants (PPR), a viral animal disease. More specifically, the invention relates to a new diagnostic method capable of detecting PPRV antibodies safely, simply and rapidly with recombinant Nucleocapsid (N) protein of PPR virus (PPRV) and specific monoclonal antibody...

20060127946 - Reading of fluorescent arrays: Reading of fluorescent arrays (103) in clinical settings is made possible by a reader (110) constructed to employ dark field illumination of the array, and mapping an image of the array onto a solid state sensor array (146) with image dimensions (D;) of the same order magnitude as the dimensions...

20060127947 - Receptor deorphanization using tagged molecular libraries: The present invention provides a method of screening test compounds and test conditions for the ability to modulate (activate or inhibit, enhance or depress) a GPCR pathway, and provides methods of assessing GPCR pathway function, such as the function of an orphan GPCR, in a cell in general. In another...

20060127951 - Screening system: Bonding data regarding a great number of combinations of ligands and analytes are obtained within a short amount of time. Management of the bonding data and the corresponding analyte is automatically performed. A ligand solution is supplied to a thin film layer of a measurement unit, prior to measurement by...

20060127955 - Assay for autoantibodies to folate receptors: The present invention identifies autoantibodies to folate receptors. Methods to identify these autoantibodies to the human folate receptors are also provided. The present invention also contemplates diagnostic methods and test kits to be used in a clinical setting for identifying a subject at risk of folate-sensitive abnormalities or disorders as...

20060127953 - Method of diagnosing osteolysis: Methods for detecting osteolysis are described. The diagnostic method involves detecting the presence of regulatory T cells in a sample from a patient....

20060127954 - N-11 truncated amyloid-beta monoclonal antibodies, compositions, methods and uses: This invention relates to antibodies, including specified portions or variants, specific for at least the human Amyloid-beta 11 N-terminal site, i.e. Aβ11-x peptides. It further provides methods of making and using said antibodies, including therapeutic formulations, administration and devices....

20060127956 - Quality control for cytochemical assays: A method and device is described for a quality control device for immunohistochemical testing of surgical biopsies. Immunohistochemistry testing involves the use of antibodies to detect the presence of proteins that have diagnostic or prognostic importance. In this invention, peptides are covalently attached to glass microscope slides. The peptides represent...

20060127958 - Immunoassay for the detection of cancer: The present invention relates to a broad cancer immunoassay. Specifically, an immunoassay for peptides associated with oncogenic processes such as metastatic proteolysis is disclosed. In an illustrative embodiment, the immunoassay utilizes antibodies which bind to peptides which are generated by proteolytic processes and which contain epitopes which are masked in...

20060127957 - Novel biologicalcancer marker and methods for determining the cancerous or non-cancerous phenotype of cells: The present invention relates to the use of the frequency value of filopodia generation in a sample cell population as a biological marker for determining the cancerous, metastatic cancerous or normal phenotype of said cell population. It also relates to a method for determining in vitro the cancerous, metastatic cancerous...

20060127959 - Antibodies that bind m. tuberculosis polypeptides: Purified antibodies that bind to M. tuberculosis ERP protein are disclosed. In one a embodiment, a purified antibody, which binds specifically with a polypeptide comprising SEQ ID NO: 39 or SEQ ID NO: 41 is provided. In some embodiments the polypeptide has a theoretical molecular weight of about 28 kDa....

20060127961 - Diagnostic assays for determination of dental caries susceptibility: The invention overcomes the limitations of the prior art by providing rapid assays for predicting the likelihood of caries development in patients. The assays allow implementation of appropriate dental care measures during a patient visit depending on the results of the assay. The assay utilizes the finding that caries-free children...

20060127960 - Process for preparation of an agglutination reagent for rapid detection of typhoid: A process for the preparation of an agglutination reagent for rapid and early detection of typhoid, comprising the steps of: preparing Salmonella typhi specific antibody; preparing latex particles suspension; and coating said latex particles with the antibody....

20060127962 - Biomarkers for intro-amniotic inflammation: Biomarkers have been discovered that are capable of identifying intra-amniotic inflammation. A single biomarker or combination of biomarkers can be used to qualify the risk of preterm delivery in a patient, provided that at least one of the biomarkers is a calgranulin, preferably calgranulin A or C. The result is...

20060127963 - Microarray-based analysis of rheumatoid arthritis markers: A microarray and method of screening for rheumatoid arthritis (RA) in a mammal is disclosed. The method comprises contacting a sample from the mammal to an immobilized polypeptide or fragment thereof homologous to at least a portion of an RA marker protein, and detecting binding of an antibody from the...

20060127964 - Microprocessors, devices, and methods for use in monitoring of physiological analytes: Described herein are microprocessors, devices, and methods useful for sweat and/or temperature detection that correlate more closely with changes in amperometric or charge signals related to analyte amount or concentration. The present invention provides methods for the establishment of more accurate sweat and/or temperature thresholds and new methods of compensation,...

20060127965 - Bindingzyme arrays and high-throughput proteomic methods: Provided herein are methods for identifying the presence or absence of a polypeptide variance between different biological samples and corresponding methods for generating a high-throughput screen to rapidly identify variances of one or more polypeptides in different biological samples. In particular, a variance in a post-translational modification on a particular...

20060127966 - Method of collecting gas-borne viable matter: A method of collecting viable matter included in a gaseous atmosphere includes directing a flow of gas toward a suspension medium. The suspension material is configured to maintain viable matter in a living state. The method also includes capturing viable matter carried in the gas in the suspension material and...

20060127967 - In vitro test for studying compound predicting pharmacologic and/or harmacokinetic and/or pharmacodynamic parameters of a compound: The invention provides a method and an assay for predicting the pharmacologic and/or pharmacokinetic and/or pharmacodynamic activity and/or effective concentration of a test material using cell and/or protozoa and/or micro-organism by assessing the effect of the test material on the and/or protozoa and/or micro-organism. Further, the invention provides an apparatus...

20060127968 - Protozoan rhomboid proteins: Protozoan Rhomboid Proteins This invention relates to the identification of protozoan Rhomboid proteins that are involved in the invasive processes of protozoan pathogens, such as P. falciparum. Modulation of these Rhomboid proteins may thus be useful in treating protozoan pathogen infection. Methods and means relating to the modulation of protozoan...

20060127969 - Assays for trichomonal and other hydrolases: The release by trichomonads of a hydrolase that hydrolyzes a narrowly defined class of substrates at a low pH without interference from hydrolases that are unrelated to trichomoniasis is the basis for a selective diagnostic assay for trichomoniasis that measures hydrolysis of any of these substrates by vaginal fluid at...

20060127970 - Controlling proteolytic action in natural rubber latex: A method for controlling proteolytic action in manufacturing enzyme-treated natural rubber latex and products made therefrom. The method involves adding a protease inhibitor such that hydrolysis by protease enzymes is stopped. A preferred inhibitor is a low molecular weight, cationic protease inhibitor from Fagopyrum esculentum having stability in the pH...

20060127971 - Process for protection of insoluble enzymatic biiocatalysts, biocatalyst obtained thereof and bioreactor with the immobilized biocatalyst: The purpose of the present invention is to provide a process for protection of enzymatic biocatalysts, constituted by a primary support, wrapped by a secondary matrix. More particularly, the invention is embodied as a bioreactor with a configuration that preserves the physical integrity and catalytic activity of the biocatalyst after...

20060127972 - In vivo production of cephalosporins: The invention provides improved in vivo production of acylated cephalosporins with higher yield using expandase which is predominantly localised in the cytosol of the host cell....

20060127979 - Chimeric human papillomavirus (hpv) l1 molecules and uses therefor: Disclosed are chimeric HPV L1 proteins and virus like particles comprising the same which are capable of generating high titer neutralizing antibody responses against at least two HPV types. The disclosed chimeric HPV L1 proteins and VLPs are useful as therapeutic and prophylactic reagents, as well as reagents for diagnosing...

20060127982 - Development of an asporogenic bacillus licheniformis and production of keratinase therefrom: A recombinant bacteria and methods of making and using the same are provided. The recombinant bacteria is a recombinant Bacillus having an inactive endogenous spoIIAC gene, with said recombinant Bacillus producing greater quantities of keratinase than a corresponding wild-type Bacillus that does not have said inactive endogenous spoIIAC gene inserted...

20060127973 - Dna sequences of major secreted proteins from the ciliate tetrahymena and use thereof: Furthermore a method is disclosed for the heterologous expression of proteins from Tetrahymena using gene constructs made from regulatory elements selected from the group consisting of promoters or terminators from Tetrahymena and coding nucleic acid sequences of a protein to be expressed heterologously, said regulatory elements from Tetrahymena being obtainable...

20060127980 - Human excitatory amino acid transporter-2 gene promoter and uses thereof: The nucleic acid sequence of the human Excitatory Amino Acid Transporter-2 Gene (hEAAT2) promoter, a nucleic acid sequence that hybridizes to the hEAAT2 promoter nucleic acid sequence under stringent hybridization conditions, and a nucleic acid sequence that is functionally equivalent to the hEAAT2 promoter sequence are provided, as are vectors...

20060127977 - Isolated (t1r2/t1r3) sweet taste receptors that respond to sweet taste stimuli: Isolated or biologically pure taste receptors comprised of at least one T1R2 and T1R3 polypeptide and compositions containing are provided. These taste receptors specifically bind and/or are specifically activated by sweet taste stimuli such as natural and artificial sweetener compounds. These taste receptors are useful in screening assays for identifying...

20060127975 - Method for culturing cells in order to produce substances: The invention concerns a method for culturing cells in order to produce substances. According to the invention a cell line producing substances is cultured while feeding a nutrient medium in such a manner that glucose limitation occurs in the culture solution. The degree of glucose limitation DGL=qGlc/qGlcmax (qGlc=observed current specific...

20060127984 - Modified vaccinia virus ankara for the vaccination of neonates: The invention concern the use of a virus for the preparation of a medicament for the vaccination or treatment of a neonatal or prenatal animal, including a human, wherein the virus is capable of infecting the cells of the neonatal or prenatal animal, including a human, but not capable of...

20060127974 - Mpg added to fermentation: A method for recovering a protein of interest from a culture solution comprising: adding a polyol having the formula CnH2n+2On, wherein n is from 4 to 8, and/or a carbohydrate, and/or a derivative thereof, to the culture solution during recovery processing....

20060127978 - Nucleic acid recombination method, host cell and expression vector: This invention is to provide a nucleic acid recombination method which enables to carry out precisely homologous recombination of a desired target region, a host cell which enables to carry out precisely homologous recombination of a desired target region, and an expression vector which can be used for the nucleic...

20060127983 - Secreted and transmembrane polypeptides and nucleic acids encoding the same: The present invention is directed to secreted and transmembrane polypeptides and to nucleic acid molecules encoding those polypeptides. Also provided herein are vectors and host cells comprising those nucleic acid sequences, chimeric polypeptide molecules comprising the polypeptides of the present invention fused to heterologous polypeptide sequences, antibodies which bind to...

20060127981 - Targeting viruses using a modified sindbis glycoprotein: The present invention relates to viruses that are engineered to contain a surface ligand molecule which targets the virus to a cell of interest. In particular non-limiting embodiments, the cell of interest is desirably ablated and may be a cancer cell, an infected cell, a cell exhibiting a non-malignant proliferative...

20060127976 - Use of oxalate deficient aspergillus niger strains for producing a polypeptide: The invention relates to oxalate deficient A. niger strains for the production of a given enzyme, wherein the oxalate deficient strain produces at least the same amount of enzyme as the wild type strain it originates from under the same culture conditions. Preferably, the oxalate deficient A. niger strain produces...

20060127985 - Cytokine designated 4-1bb ligand and human receptor that binds thereto: Novel 4-1BB ligand (4-1BB-L) polypeptides and a human cell surface receptor designated 4-1BB that binds 4-1BB-L are provided. Isolated 4-1BB-L-encoding and human 4-1BB-encoding DNA sequences, recombinant expression vectors comprising the isolated DNA sequences, and host cells transformed with the recombinant expression vectors are disclosed, along with methods for producing the...

20060127986 - Recombinant production of novel polyketides: Novel polyketides and novel methods of efficiently producing both new and known polyketides, using recombinant technology, are disclosed. In particular, a novel host-vector system is described which is used to produce polyketide synthases which in turn catalyze the production of a variety of polyketides....

20060127987 - Microorganism producing hyaluronic acid and purification method of hyaluronic acid: The present invention relates to a hyaluronic acid producing strain Streptococcus sp. KL0188 and a method for purifying hyaluronic acid, more particularly to a Streptococcus sp. KL0188 that does not express hyaluronidase and is non-hemolytic, and a method for purifying hyaluronic acid using an aromatic adsorption resin and an active...

20060127989 - Antigenic fragment of human t-lymphotropic virus: Antigenic fragments of human T-lymphotropic virus (HTLV), their fusion proteins with glutathione Stranferase (GST) or thioredoxin (Thio), and a process for producing the fusion proteins thereof. The antigenic fragment of HTLV comprises the amino acid sequence of SEQ ID Nos: 3 or 4....

20060127988 - Synthetic nucleic acid molecule compositions and methods of preparation: A method to prepare synthetic nucleic acid molecules having reduced inappropriate or unintended transcriptional characteristics when expressed in a particular host cell....

20060127990 - 4'-thionucleotide: m

20060127991 - Process for making de-esterified pectins their cmposition and uses thereof: The present invention relates to a process by which a pectin extract is first de-esterified using a biocatalyst. Secondly, the resulting high molecular weight de-esterified pectin is further de-esterified and optionally amidated using conventional methods. Since the bio-catalyst may de-esterify to a DE of about 25%, conventional acid or alkali...

20060127992 - Microorganism and process for preparing vitamin b6: Disclosed is a recombinant microorganism of the genus Sinorhizobium which is transformed with a vector containing pyridoxol 5′-phophate synthase gene and D-erythrose 4-phosphate dehydrogenase gene, and being capable of producing vitamin B6....

20060127993 - Thiamin production by fermentation: The present invention provides a method for producing thiamin products using a microorganism containing a mutation that causes it to overproduce and release thiamin products into the medium. Biologically pure cultures of the microorganisms and isolated polynucleotides containing the mutations are also provided. In addition, methods for detecting a pathogenic...

20060127994 - Aldehyde dehydrogenase gene: The present invention relates to a novel DNA which encodes aldehyde dehydrogenase (SNDH), an expression vector containing the DNA and recombinant organisms containing said DNA. Furthermore, the present invention concerns a process for producing recombinant aldehyde dehydrogenase protein and a process for producing L-ascorbic acid (vitamin C) and/or 2-keto-L-gulonic acid...

20060127995 - Enzymatic reactions in the presence of keto acids: Conversion in vitro of X-Gly to X-alpha-hydroxy-Gly or X—NH2 (X being a peptide or any other compound having a carbonyl group capable of forming a covalent bond with glycine) is accomplished enzymatically in the presence of keto acids, or salts or esters thereof, to provide a good yield without the...

20060127996 - Method of extraction of isothiocyanates into oil from glucosinolate-containing plants and method of producing products with oil containing isothiocyanates extracted from glucosinolate-containing plants: A method of extraction of isothiocyanates into the oil from glucosinolate-containing plants. A method of preparing products including pharmaceutical compositions, food or drink products, supplements or additives, skin or hair products, and agricultural products which contain isothiocyanate oil extracted from glucosinolate-containing plants....

20060127997 - Process for the preparation of compounds with enhanced optical purity: A process for preparation of a compound with enhanced optical purity is disclosed wherein a mixture of the enantiomers of a chiral amine is brought into contact with an enzyme having peptide deformylase activity with a bivalent metal ion as a cofactor....

20060127998 - Method of purifying 3-hydroxyalkanoic acid copolymer: The object of this invention is to provide a method for purifying PHA separated from PHA-containing microbial cells in high purity without incurring any serious decrease in molecular weight. The present invention provides a method for purifying a 3-hydroxyalkanoic acid copolymer produced by a microorganism, which comprises treating an aqueous...

20060127999 - Process for producing ethanol from corn dry milling: A process for producing ethanol by the conversion of carbohydrates from a corn dry milling process in which the bottoms fraction from distillation of ethanol in a conventional yeast fermentation is used in a process including a combination of biochemical and synthetic conversions. The process results in high yield ethanol...

20060128000 - Enone reductase gene and microbial production of levodione: Disclosed is an isolated DNA comprising a nucleotide sequence coding for an enzyme having enone reductase activity wherein the enzyme is characterized by the following physico-chemical properties: (a) molecular mass: 61,300±5,000 Da (estimated using gel filtration, consisting of one subunit); (b) co-factor: NADPH and NADH; (c) substrate specificity: active on...

20060128001 - Highly efficient hydrogen production method using microorganism: It is intended to obtain anaerobic microbial cells in an amount sufficient for hydrogen generation reaction, impart a hydrogen generation function to an aerobic microorganism within a short time and provide an industrial advantageous method of producing hydrogen. The above object can be established by providing a highly efficient microbial...

20060128002 - Method for supplying a biologically utilized aqueous system with oxygen, and device and set for carrying out said method: The invention relates to a method for supplying a biologically utilized aqueous system with oxygen, comprising the following steps: i) a predetermined quantity of catalase is added to the biologically utilized aqueous system; ii) H2O2 or a salt thereof is added to the biologically utilized aqueous system; and iii) the...

20060128003 - Therapeutic agents for low hdl-cholesterolemia: The present invention is to provide an agent for low HDL-cholesterolemia, a prophylactic and/or therapeutic antiarteriosclerosis agent as well as a method for preventing and treating low HDL-cholesterolemia, arteriosclerosis and their related diseases or disorders, with emphasis given to improvement in HDL, without resorting to genetic engineering technology. Further, the...

20060128004 - Process and an apparatus for producing calcium carbonate via an enzymatic pathway: The invention relates to a method for improving the production yield of precipitated calcium carbonate (PCC). The improvement resides in the use of an enzymatic bioreactor that supplies HCO3 into the reaction enabling to produce PCC without bubbling gaseous carbon dioxide in the aqueous solution. The present invention has an...

20060128005 - Observation apparatus: An observation apparatus includes a sample tray that holds a container housing a cultured cell and a culture medium and covered with a lid; an observation unit that serves for observation of the cultured cell; and a shifting unit that relatively shifts the sample tray and a light axis of...

20060128006 - Hydrodynamic capture and release mechanisms for particle manipulation: A cell analysis and sorting apparatus is capable of monitoring over time the behavior of each cell in a large population of cells. The cell analysis and sorting apparatus contains individually addressable cell locations. Each location is capable of capturing and holding a specified number of cells, and selectively releasing...

20060128007 - Device and method for multiple analyte detection: The invention is directed to a method and device for simultaneously testing a sample for the presence, absence, and/or amounts of one or more of a plurality of selected analytes. The invention includes, in one aspect, a device for detecting or quantitating a plurality of different analytes in a liquid...

20060128008 - Gas-borne matter collection device: A plate for use in a gas-borne matter collection device includes a substance provided on a surface of the plate for preserving viable matter in a living state without generally promoting growth of the viable matter....

20060128009 - Instrument for monitoring polymerase chain reaction of dna: An optical instrument monitors PCR replication of DNA in a reaction apparatus having a temperature cycled block with vials of reaction ingredients including dye that fluoresces in presence of double-stranded DNA. A beam splitter passes an excitation beam to the vials to fluoresce the dye. An emission beam from the...

20060128010 - Method for preparing engineered tissue: A method for preparing a human or animal tissue by applying a compressive force to a stack of sheets of living tissue thereby inducing adjacent layers to fuse or adhere to each other. The force is applied in direction normal to the surface of the tissue. A multi-layer tissue produced...

20060128011 - Replication of hepatitis c virus in non-hepatic epithelial and mouse hepatic cells: Cells and cell lines which replicate HCV of non-hepatic human and non human origin are disclosed. Also provided are methods of using such cells and cell lines to identify anti-HCV agents for the treatment of HCV infection....

20060128013 - Directed differentiation of embryonic cells: Methods are described for mapping a pathway of differentiation of a population of embryonic cells that includes exposing the cells to an exogenous factor and measuring gene expression products that are characteristic of a particular cell type or lineage. Directing differentiation of human embryonic cells relies on dissociated embryoid bodies...

20060128012 - Substrate recognition by differentiable human mesenchymal stem cells: The invention described herein provides a structure for growing isolated differentiable human mesenchymal cells, which includes a three-dimensional matrix of fibers. The matrix serves as an implantable scaffolding for delivery of differentiable human mesenchymal cells in tissue engineering. The invention further provides compositions that contain the three-dimensional matrix of fibers...

20060128014 - Compositions and methods for culturing stem cells: The present invention provides methods of culturing, propagating, treating, and maintaining stem cells in the presence of a phosphate mimic. In particular, the invention relates to the propagation of stem cells in vitro, the formation of neurospheres in vitro, and to tissue culture of stem cells in the presence of...

20060128015 - Small hepatocyte-rich colonies, process for preparing the colonies, process for maturating the colonies into liver tissue and method of estimating effects of drug by using matured small hepatocyte-rich colonies: The present invention provides a process for preparing a transplantable liver tissue, cell colonies suitable for the process, and a process for preparing them. At least about 70 % of the cells in the small hepatocyte-rich colonies of the present invention are small hepatocytes. In particular, each colony comprises about...

20060128016 - Fibrin-containing composition: It is intended to provide a scaffold material having favorable properties and being appropriate for cell proliferation and differentiation in regeneration therapy. Namely, a fibrin-containing biological scaffold material to be used in the case of employing a fibrin composition for the regeneration of a human tissue and cell proliferation, characterized...

20060128018 - Directed genetic modifications of human stem cells: Human embryonic stem cells can be genetically transformed by a combination of electroporation and homologous recombination. This technique makes it possible to create targeted inserts or deletions to the genome of the stem cells. This ability makes it possible to create populations of progeny cells which have differentiated into a...

20060128017 - Process for the production of an active molecule vector used to diffuse active substances and vector thus obtained: Adding glutaraldehyde, OHC—(CH2)3—COH, and Awaiting the polycondensation reaction and the formation of imines, and Recovering the poly(monomer-G) that is obtained. The monomer is selected from among the L-ornithine, the L-lysine or the L-citrulline. Further described are the biomedical vector that is obtained, and the use as a vector of active...

20060128020 - Methods of unidirectional, site-specific integration into a genome, compositions and kits for practicing the same: The subject invention provides a unidirectional site-specific integration system for integrating a nucleic acid into the genome of a target cell. The provided system includes a site-specific integrating expression cassette (INTEC) vector, consisting of (a) a polynucleotide of interest operably linked to a promoter, (b) a single recombination site, and...

20060128019 - Process for producing virus vector containing membrane protein having sialic acid-binding in envelope with the use of gram-positive bacterium origin nueraminidase: The present invention provides methods for producing a viral vector comprising a membrane protein that binds to sialic acid as a component of the envelope, using neuraminidase (NA) derived from Gram-positive bacteria. The methods comprise the steps of culturing cells producing a viral vector in the presence of an NA...

20060128021 - Unusual retrotransposon from the yeast candida albicans: TCa2 is a TyI/copia retrotransposon from the pathogenic yeast Candida albicans. In contrast to other retrotransposons it can appear as an abundant, extrachromosomal double-stranded DNA molecule, called pCal. The invention relates to the isolation and characterisation of TCa2 and pCal together with its uses for inducing random mutagenesis in a...

20060115805 - Gelatine-based materials as swabs: A swab comprising gelatine or collagen has been found to have a remarkably high recovery of microorganisms. Furthermore, the samples, such as microorganisms, spores, nucleotides and other biologically or biochemically relevant compounds can be fully recovered from the collagen- or gelatine-comprising swab. The invention thus provides a method and swab...

20060115804 - Method of studying living cells: The present invention provides a method of eliciting a Raman signal from a living cell, or a plurality of living cells, said method comprising irradiating the cell with a laser having a wavelength of 785±60 nm....

20060115807 - Episomal expression system: A cell which expresses a replication factor is transfected with a vector which requires the presence of the replication factor to be maintained episomally within the cell. This is then expanded into a plurality of cells; and a cell in the plurality of cells selected (i) which maintains the vector...

20060115806 - Global analysis of transposable elements as molecular markers of cancer: The present invention provides methods of determining expression patterns, methylation patterns and chromatin status patterns for transposable element gene sequences. These methods can be utilized to diagnose, stage and treat cancer....

20060115808 - Microarrays of functional biomolecules, and uses therefor: Disclosed are products and methods to facilitate the identification of compounds that are capable of interacting with biological macromolecules of interest, especially when such macromolecules are attached to a support surface in microarray. Aspects of the invention concern attachment chemistry, peptide labeling, antibody preparation, applications and so on....

20060115809 - Microarrays of functional biomolecules, and uses therefor: Disclosed are products and methods to facilitate the identification of compounds that are capable of interacting with biological macromolecules of interest, especially when such macromolecules are attached to a support surface in microarray. Aspects of the invention concern attachment chemistry, peptide labeling, antibody preparation, applications and so on....

20060115810 - Microarrays of functional biomolecules, and uses therefor: Disclosed are products and methods to facilitate the identification of compounds that are capable of interacting with biological macromolecules of interest, especially when such macromolecules are attached to a support surface in microarray. Aspects of the invention concern attachment chemistry, peptide labeling, antibody preparation, applications and so on....

20060115846 - Antigenic binding patterns of norovirus to human histo-blood group antigens: The invention provides a compound which competitively inhibits the binding of a norovirus with a native blood antigen of a human host, as well as a kit for determining whether an individual has been infected by a norovirus. Also provided is a method for determining the susceptibility of an individual...

20060115851 - Artificial genes for use as controls in gene expression analysis systems: Method of producing universal controls for use in gene expression analysis systems such as macroarrays, real-time PCR, northern blots, SAGE and microarrays. The controls are generated either from near-random sequence of DNA, or from intergenic or intronic regions of a genome. Twenty-three specific control sequences are also disclosed. Also presented...

20060115812 - Biochemical markers of the human endometrium: Endometrium secreted polypeptides are assayed in body fluids to determine the presence of polypeptides of specified pI and MW values that have been found to be regulated in body fluids according to the status of the endometrium, or the presence of hyperplasia or adenocarcinoma....

20060115837 - Crystals and structure of domain iii of west nile virus envelope protein in association with a fab fragment of a neutralizing antibody: The present invention provides crystalline forms of domain III of the West Nile Virus (“WNV”) envelope protein in complex with a Fab fragment of a neutralizing antibody, methods of obtaining such crystals and high-resolution structures and atomic structure coordinates. The crystals of the invention and the atomic structural information are...

20060115811 - Cytochrome p450ra1-2 and related proteins: The present invention provides a novel all-trans-RA inducible all-trans-RA metabolizing cytochrome P450, P450RAI-2, that is predominantly expressed in the brain, cerebellum in particular. Human P450RAI-2 show 42% amino acid identity to human P450RAI-1 and when transfected into COS-1 cells causes the rapid conversion of all-trans-RA into more polar metabolites including...

20060115828 - Detection device having increased detection rate, and method for quick detection of biological molecules: A biological molecule detection device that includes a detection array, arranged on a body and having one or more probes for detecting corresponding electrically charged molecules, wherein a time varying electric field generating circuit is provided for generating at least one time varying electric field around the detection array within...

20060115832 - Detection of cancer cells in body fluids: A method of detecting circulating melanoma or carcinoma cells in a subject. The method comprises obtaining a body fluid from a subject and detecting the expression of a panel of genes in the body fluid, wherein the expression of the panel of genes indicates the presence of circulating melanoma or...

20060115823 - Detection reagent for shiga toxin family gene of entero-hemorrhagic escherichia coli: The present invention provides a combination of oligonucleotides preferable for composing a rapid and specific gene testing reagent for Shiga toxin family gene type 2 (stx) of entero-hemorrhagic Escherichia coli (EHEC). More specifically, the present invention provides a method for detecting stx2 RNA of EHEC by specifically amplifying only stx2...

20060115819 - Detection, identification and differentiation of eubacterial taxa using a hybridization assay: The present invention relates to a method for the specific detection and/or identification of Staphylococcus species, in particular Staphylococcus aureus, using new nucleic acid sequences derived from the ITS (Internal Transcribed Spacer) region. The present invention relates also to said new nucleic acid sequences derived from the ITS region, between...

20060115818 - Diagnostic and therapeutic means for kidney stone related pathologies: A description is given of a nucleic acid comprising at least one fragment of the human gene ZNF365 or functional portions thereof for use in the diagnosis or treatment of pathologies associated with kidney stones. Appropriate diagnostic kits are also described....

20060115843 - Diagnostic test for collie eye anomaly: The invention relates to a method for identifying dogs which are genetically normal, heterozygous for, or homozygous for the mutation primarily responsible for Collie eye anomaly (CEA). The method comprises the steps of obtaining a biological sample from a dog and testing DNA in the biological sample for the presence...

20060115844 - Enhanced amplifiability of minute fixative-treated tissue samples, minute stained cytology samples, and other minute sources of dna: The specification provides materials and methods for enhancing the amplifiability of nucleic acids that are present in minute amounts. Preferably, the materials and methods are utilized in combination with pathology techniques to amplify nucleic acids obtained from fixative and/or stained tissue or cell samples. The amplified nucleic acids can then...

20060115826 - Gene expression profiling for identification monitoring and treatment of multiple sclerosis: A method is provided in various embodiments for determining a profile data set for a subject with multiple sclerosis or inflammatory conditions related to multiple sclerosis based on a sample from the subject, wherein the sample provides a source of RNAs. The method includes using amplification for measuring the amount...

20060115827 - Genetic markers for predicting disease and treatment outcome: The invention provides compositions and methods for determining the increased risk for recurrence of certain cancers and the likelihood of successful treatment with one or both of chemotherapy and radiation therapy. The methods comprise determining the type of genomic polymorphism present in a predetermined region of the gene of interest...

20060115842 - Highly sensitive homogeneous assay based on anti-stokes' shift fret measurement: This invention relates to a novel time-resolved lanthanide-based energy transfer assay utilizing non-overlapping acceptor fluorophores, having their absorption maximum energetically at higher level than the main emittive energy level of the donor. In this assay the lifetime of the energy transfer enhanced acceptor signal is partially independent of the energy...

20060115852 - Human p51 genes and gene products thereof: Novel human genes falling within the category of family genes relating to p53 gene which is known as a cell proliferation regulatory gene, and gene products thereof. A human p51 gene characterized by containing a base sequence encoding an amino acid sequence represented by SEQ ID NO:1; a human p51...

20060115840 - In situ dilution of external controls for use in microarrays: An array in which an external control feature for normalization has been designed and tested for its ability to mimic the range of observed expression levels for a test set of oligos. The external control probes span a series of concentrations. They are spatially randomized across a grid of an...

20060115813 - Intracellular signaling molecules: Various embodiments of the invention provide human intracellular signaling molecules (INTSIG) and polynucleotides which identify and encode INTSIG. Embodiments of the invention also provide expression vectors, host cells, antibodies, agonists, and antagonists. Other embodiments provide methods for diagnosing, treating, or preventing disorders associated with aberrant expression of INTSIG....

20060115831 - Map: The invention provides map polypeptides and polynucleotides encoding map polypeptides and methods for producing such polypeptides by recombinant techniques. Also provided are methods for utilizing map polypeptides to screen for antibacterial compounds....

20060115833 - Method and apparatus for determining base sequence of nucleic acid molecule: Disclosed herein is a method and apparatus for determining the base sequence of a nucleic acid molecule by cleaving a nucleic acid molecule of interest while controlling the cleavage site, measuring the change in mass which occurs in the nucleic acid molecule after the cleavage step, and acquiring the base...

20060115815 - Method and compositions for identifying anti-hiv therapeutic compounds: Methods are provided for identifying anti-HIV therapeutic compounds substituted with carboxyl ester or phosphonate ester groups. Libraries of such compounds are screened optionally using the novel enzyme GS-7340 Ester Hydrolase. Compositions and methods relating to GS-7340 Ester Hydrolase also are provided....

20060115841 - Method and system for elucidating the primary structure of biopolymers: The mass spectra are classified in dependence on results of the first sequence analysis (30), resulting in at least one first spectrum class, to which a known biopolymer can be assigned, and one second spectrum class, to which no known biopolymer can be assigned, being obtained. A further analysis (50)...

20060115835 - Method for the carry-over protection in dna amplification systems targeting methylation analysis achieved by a modified pre-treatment of nucleic acids: Particular aspects provide methods for specific amplification of template DNA in the presence of potentially contaminating PCR products from previous amplification experiments. Particular embodiments comprise, in a first step, contacting DNA with a bisulfite solution, which sulfonates unmethylated (but not methylated) cytosines, resulting in cytosine deamination and generation of sulfonated...

20060115850 - Method for the synthesis of dna fragments: The invention relates to a method that can be carried out in parallel and automated for the production of any nucleic acid, comprising the following steps: a) coupling an oligonucleotide to a solid matrix b) adding an additional oligonucleotide c) ligating the oligonucleotides from steps a) and b) in one...

20060115829 - Method of treating a cancer: The present invention provides isolated nucleic acids encoding delta DNA methyltransferase 3B molecules that are involved in the treatment and prevention of cancers such as, but not limited to, lung cancer. The delta DNA methyltransferase 3B molecules of the present invention are found to play a critical role in promoter-specific...

20060115845 - Methods and compositions for correlating genetic markers with cardiovascular disease: The present invention provides methods of identifying a subject having an increased or decreased risk of developing cardiovascular disease, comprising: a) correlating the presence of one or more genetic markers in chromosome 3q13.31 with an increased or decreased risk of developing cardiovascular disease; and b) detecting the one or more...

20060115814 - Methods and compositions, relating to hiv gp41 antigens and other hiv envelope antigens: The present invention concerns methods and compositions involving nondenatured IIIV gp41 antigens alone and in combination with other nondenatured HIV envelope antigens for the detection of early antibodies against HIV. Such methods and compositions may be used to detect HIV infection in a patient or in a blood sample. The...

20060115830 - Methods for monitoring genomic dna of organisms: The invention provides novel SGP primers for improved use in waveform-profiling methods of DNA amplification. In one embodiment, use of an SGP primer in a method of DNA amplification results in exponential amplification of several distinct products. In another embodiment, the methods of the invention further comprise a novel half-time...

20060115839 - Methods of identifying an agent that modulates an interaction between 53bp1 and histone h3, and uses thereof: The present invention relates to methods of identifying modulators of an interaction between 53BP1 and histone H3 (H3). The present invention also relates to methods of use of inhibitors of an interaction between 53BP1 and H3. The present invention further relates to fragments of 53BP1 and H3, as well as...

20060115834 - Methods of identifying metastatic potential in cancer: The present invention encompasses methods for predicting metastasis in cancer by assessing the structure of the complement protein C1qA. The methods may encompass examining either protein or nucleic acids, and may further include making treatment decisions based on the predictive methods....

20060115848 - Nucleic acid end-labeling reagents: Compounds and methods are provided for covalent end-labeling of polynucleotides. Incorporation of a nucleic acid affinity group improves the efficiency of reaction of aldehyde reactive groups with the nucleic acid leading to more efficient labeling....

20060115825 - Pna chip using zip-codes and fabrication method thereof: A PNA zip-code chip in which PNA zip-code probes are immobilized on a substrate at high density using an epoxy compound as a linker, and method for fabricating such PNA chip. The use of PNA provides the chip with superior properties to DNA chips, allowing precise diagnosis of congenital diseases...

20060115853 - Polynucleotides, polypeptides and method for screening for useful dog candidates: A method for screening for useful dog candidates comprises a step of investigating the genotype of the allele in exon 1 or intron 2 of dog dopamine receptor D4 gene, and a step of selecting a dog with genetic aptitudes beneficial to people by making the selection based on that...

20060115820 - Primer and probe for detecting vibrio cholerae or vibrio mimicus and detection method using the same: To produce high-performance specific gene amplification primers for detecting, quantifying, or identifying Vibrio cholerae and Vibrio mimicus, having low risk of misidentification and practically sufficient amplification efficiency and amplification specificity. We have determined partial nucleotide sequences of rpoD and gyrB genes of Vibrio cholerae, Vibrio mimicus, and closely related species,...

20060115821 - Prostate specific genes and the use thereof as targets for prostate cancer therapy: Genes that are upregulated in human prostate tumor tissues and the corresponding proteins are identified. These genes and the corresponding antigens are suitable targets for the treatment, diagnosis or prophylaxis of prostate cancer....

20060115838 - Real-time detection of amplicons using nucleic acid repair enzymes: Target nucleic acid sequences can be detected during an amplification reaction in real-time. Detection methods involve conducting an amplification reaction in the presence of at least one nucleic acid repair enzyme and at least one oligonucleotide probe that contains a mismatched or repairable base sequence such that a mismatch occurs...

20060115849 - Screening methods: A mammalian polypeptide designated Inhibitory PAS Domain Protein (IPAS) is useful for the inhibition of angiogenesis and/or tumor progression. Screening methods exist to identify compounds potentially useful as medicaments for the treatment of medical conditions related to angiogenesis or tumor progression....

20060115817 - Span-xb gene and protein for the diagnosis and treatment of cancer: The present invention relates to methods for diagnosing cancer in a subject The method involves obtaining a biological sample from a subject and measuring SPAN-Xb expression level in the biological sample. The SPAN-Xb expression level in the biological sample is compared to the SPAN-Xb expression level in a control sample...

20060115847 - Specific epitope based immunological diagnosis of tuberculosis: The currently used method for immunological diagnosis of tuberculosis infection, the tuberculin skin test, is problematic for a number of reasons; it has low specificity in BCG vaccinated individuals, a high interobserver variance and requires skill to be read and interpreted. Furthermore it requires an extra visit to the clinic...

20060115816 - Splice variant cannabinoid receptor (cb1b): The invention is based on the identification of a novel splice variant of human cannabinoid 1 (CB1) receptor, termed CB1b, and its use in the identification of therapeutic agents and in the diagnosis, prevention, and treatment of CB associated disorders such as obesity, psychiatric, pain and neurological disorders....

20060115822 - Systems and methods for probe design: Systems and methods for using the same to obtain one or more probe sequences, e.g., for use on an array, are provided. Also provided are computer program products for executing the subject methods....

20060115824 - Trend analysis and statistical process control using multitargeted screening assays: Aspects of the present invention provide novel multi-targeted microbiological screening and monitoring methods having substantial utility for monitoring and control of microbial growth and contaminants, microbiological processes, predictive microbiology, and for exposure and risk assessment. Microbial markers shared by both target and index microbes are used in novel methods for...

20060115836 - Utilization of fancc, dad1, grim19 and hadhii genes for the ultimate treatment of psoriasis: Polynucleotide probes relating to the FANCC, DAD1, GRIM19 and HADHII genes are useful for characterizing biological samples and for identifying compounds suited for the treatment of psoriasis....

20060115856 - 2'-5'-oligoadenylate synthetase like protein as a biomarker for neurodegenerative disease: The present invention relates to a biomarker for neurodegenerative disease, including amyotrophic lateral sclerosis (ALS), Alzheimer's (AD), and Parkinson's (PD) disease. More particularly, the present invention relates to the identification of an 2′-5′-oligoadenylate synthetase like protein (p59 OASL) related protein as a biomarker useful for the detection, diagnosis, and differentiation...

20060115854 - Acetyl-ldl receptor related proteins and peptides as a biomarker for neurodegenerative disease: The present invention relates to a biomarker for neurodegenerative disease, including amyotrophic lateral sclerosis (ALS), Alzheimer's (AD), and Parkinson's (PD) disease. More particularly, the present invention relates to the identification of an acetyl-LDL receptor related protein as a biomarker useful for the detection, diagnosis, and differentiation of neurodegenerative disease, including...

20060115855 - Fk506-binding protein 7 related protein as a biomarker for neurodegenerative disease: The present invention relates to a biomarker for neurodegenerative disease, including amyotrophic lateral sclerosis (ALS), Alzheimer's (AD), and Parkinson's (PD) disease. More particularly, the present invention relates to the identification of the FK 506-binding protein 7, or prolylisomerase, as a biomarker useful for the detection, diagnosis, and differentiation of neurodegenerative...

20060115857 - Molecular wire injection sensors: Disclosed is a sensor for sensing the presence of an analyte component without relying on redox mediators. This sensor includes (a) a plurality of conductive polymer strands each having at least a first end and a second end and each aligned in a substantially common orientation; (b) a plurality of...

20060115858 - Trace amine 1 receptor of the african green monkey: A trace amine receptor 1 has been isolated from the genome of Cercopithecus aethiops (African green monkey). Described is the amino acid sequence of the receptor, the nucleic acid encoding the receptor, and methods for using the receptor to identify analytes that are agonists or antagonists of the receptor....

20060115859 - Tyrosylprotein sulfotransferases and methods of use thereof: Tyrosylprotein sulfotransferases and nucleic acids encoding the tyrosylprotein sulfotransferases are described. Dual isotopes of the enzyme and of the nucleic acids encoding said enzymes have been identified in human, mouse and C. elegans. The polypeptides and polynucleotides exhibit a wide range of homologies. The polynucleotides can be used to transform...

20060115862 - Anti-tenascin monoclonal antibody immunoassays and diagnostic kits: The present invention provides immunoassays for detecting a tumor in a subject, comprising producing an antibody that specifically binds to tenascin, contacting the antibody with a biological sample suspected of containing tumor cells and determining the binding of the antibody to the biological sample. The present invention further provides methods...

20060115860 - Immunologic assay for detection of autoantibodies to folate binding protein: The present invention is directed to an assay that detects autoantibodies to folate receptor and can be used in the clinical diagnostic testing of these autoantibodies in humans. Although there are other methods that exist to detect these autoantibodies, the assay described in the present invention has several features that...

20060115861 - Structure and method for releasing substance therefrom: A structure comprises at least a porous body holding a substance releasably, comprising a capping member for keeping the substance inside the pore and/or on at least a part of the entire surface of the porous body, and a connecting member for connecting the porous body and the capping member...

20060115864 - Assays for identifying ubiquitin agents and for identifying agents that modify the activity of ubiquitin agents: Provided are methods and compositions for assaying for ubiquitin agents that are enzymatic components of ubiquitin-mediated proteolysis and, more particularly, methods and compositions for assaying for agents that modulate the activity of such ubiquitin agents....

20060115863 - Inositol phosphate detection assays: Assays for detecting or measuring inositol phosphate, for detecting or measuring signaling pathways, for detecting or measuring the activity of phospholipase C-linked receptors and/or their cognate pathways, for detecting or measuring the activity of inositol kinases and inositol phosphatases, and for screening for compounds that modulate the activity of signaling...

20060115865 - Lamotrigine analogs: Generally, the present invention relates to lamotrigine analogs that have substituents at the triazine 3-position and on the benzene 4-position and 5-position. The lamotrigine analogs can include immunogenic moieties that can be used to prepare anti-lamotrigine antibodies, or antigenic moieties that can be used in immunodiagnostic assays for lamotrigine. Also,...

20060115866 - Immunoassay method and kit to be used therein: Using two types of antibodies, i.e., a first antibody having a higher affinity for a target substance than for a competitive substance and a second antibody having a higher affinity for the competitive substance than for the target substance, a specimen is treated with these two antibodies. Then, the competitive...

20060115867 - Trim 5 related protein as a biomarker of neurodegenerative disease: The present invention relates to a biomarker for neurodegenerative disease, including amyotrophic lateral sclerosis (ALS), Alzheimer's (AD), and Parkinson's (PD) disease. More particularly, the present invention relates to the identification of a tripartite motif protein TRIM5 isoform gamma related protein as a biomarker useful for the detection, diagnosis, and differentiation...

20060115868 - Activation mixture: The present invention relates to an activation mixture comprising factor-deficient substrate plasma, an activator, and a phospholipid. The present invention also relates to a method for preparing, and the use of, an activation mixture. The present invention further relates to an assay method for determining the amount of blood coagulation...

20060115870 - High throughput assay for human rho kinase activity: The present invention provides a high throughput assay for human Rho kinase activity in vitro, and methods and kits therefor. A high throughput method of assaying a test compound for human Rho kinase modulating activity is also provided....

20060115869 - Uses of carbamoyl phosphate synthetas for the diagnosis of inflammatory diseases and sepsis: Use of carbamoyl phosphate synthetase 1 (CPS 1) and/or of fragments of the N-terminal part of CPS 1 from body fluids or body tissues as marker peptides for the diagnosis and for the prognosis and the monitoring of inflammations and infections, including sepsis, and of liver failure as part of...

20060115871 - Protein interaction reporter agents and methods for using same: Particular aspects provide novel protein interaction reporter (PIR) compounds (e.g., formulas I and II), comprising at least two protein reactive moieties (e.g., N-hydroxysuccinamide), each linked to a reporter moiety (e.g., mass reporter) by a covalent labile bond that is differentially cleavable with respect to peptide bonds (e.g., by a method...

20060115872 - Culture medium and a method for detection of parasites: This invention relates to a culture medium, a kit containing the culture medium and to a method for detection of a parasite such as Dientamoeba fragilis and/or another parasite. The culture medium of the invention is bi-phasic and includes a solid phase containing an egg slope or agar slope; and...

20060115873 - Novel exothermic material, method and device for lysing cells using the exothermic material: Provided are an exothermic material, and a method and device for lysing cells using the exothermic material. The exothermic material is a solid material containing CaO, 5-7% by weight of MgCl2 or MgSO4, and 5-7% by weight of NaOH, with respect to the total weight of CaO, and generates heat...

20060115875 - Detection, characterization and treatment of viral infection and methods thereof: A method of detecting, characterizing and treating viral infection is provided. In particular, a strategy of molecular mimicry is provided for characterizing viral behavior and/or a predisposition for a given viral outcome in vivo. Novel compositions are also provided for detecting, characterizing and treating viral infections....

20060115874 - Enrichment method for variant proteins with altered binding properties: A method for selecting novel proteins such as growth hormone and antibody fragment variants having altered binding properties for their respective receptor molecules is provided. The method comprises fusing a gene encoding a protein of interest to the carboxy terminal domain of the gene III coat protein of the filamentous...

20060115877 - Hyaluronate synthase gene and uses thereof: Disclosed are DNA sequences encoding hyaluronic acid synthase that are employed to construct recombinant cells useful in the production of hyaluronata synthase and hyaluronic acid (HA). In preferred aspects, chromosomal DNA encoding the HA synthase gene, hasA, was cloned from a Streptococcus pyoganas genomic library. These vectors were used to...

20060115876 - Site-directed modification of fviii: This invention relates to Factor VIII muteins that are covalently bound, at a predefined site that is not an N-terminal amine, to one or more biocompatible polymers such as polyethylene glycol. The mutein conjugates retain FVIII procoagulant activity and have improved pharmacokinetic properties....

20060115878 - Method for producing l-amino acid: In a method for producing an L-amino acid by culturing a microorganism having an ability to produce an L-amino acid in a medium to produce and accumulate the L-amino acid in the medium and collecting the L-amino acid from the medium, a Gram-negative bacterium having the Entner-Doudoroff pathway and modified...

20060115879 - Process for producing anticancer agent ll-d45042:

20060115880 - Enzymatic production of acyl flavonoid derivatives: Disclosed is a method for enzymatic synthesis of esters of flavonoid and flavonoid derivatives, according to which a reaction medium containing an organic solvent, a glycosylated flavonoid or aglycon flavonoid, an acyl group donor, and an enzymatic catalyst is produced, b) additional quantities of flavonoid and/or acyl donor are optionally...

20060115881 - High eicosapentaenoic acid producing strains of yarrowia lipolytica: Engineered strains of the oleaginous yeast Yarrowia lipolytica capable of producing greater than 25% eicosapentaenoic acid (EPA, an ω-3 polyunsaturated fatty acid) in the total oil fraction are described. These strains comprise various chimeric genes expressing heterologous desaturases, elongases and acyltransferases and optionally comprise various native desaturase and acyltransferase knockouts...

20060115882 - Method for producing symmetric triglycerides: A method for producing symmetric triglycerides, which comprises the steps of reacting a medium chain fatty acid triglyceride with a long chain fatty acid triglyceride in the presence of an enzyme or a chemical catalyst to conduct random transesterification reaction and thereby to obtain a reaction product containing a triglyceride...

20060115883 - Preparation of (e)- and (z)-2-methyl-2-butenoic acids: A method has been developed to prepare (E)- and (Z)-2-methyl-2-butenoic acids (2M2BA) from a mixture of (E,Z)-2-methyl-2-butenenitriles (2M2BN) by the regioselective hydrolysis of (E)-2M2BN to (E)-2-methyl-2-butenoic acid (2M2BA) using enzyme catalysts having either a nitrilase activity or a combination of nitrile hydratase and amidase activities. The method provides high yields...

20060115884 - Ethanol fermentation using oxidation reduction potential: A process to improve ethanol yield, decrease fermentation time and reduce byproduct formation by monitoring and controlling oxidation reduction potential (redox) of the fermentor is disclosed....

20060115885 - Technique of removing marker gene by transient expression of site-specific recombinase gene: The present inventors conceived that a marker gene may be efficiently and easily removed by using the floral dip method to transiently express high levels of a site-specific recombinase gene. it is considered that the present methods can also be applied to methods for transposing a transposon that does not...

20060115886 - Saponin-decomposing enzyme, gene thereof and large-scale production system for producing soyasapogenol b: The present invention provides a protein having saponin-decomposing activity, more specifically a protein which can decompose a glycoside having soyasapogenol B as an aglycone to produce soyasapogenol B, a polynucleotide encoding such a protein, and a method of producing soyasapogenol B on a large scale using the same. A protein...

20060115887 - Method for the biological breakdown of effluent containing nitroaromatics: A method for the biodegradation of nitroaromatic-containing wastewater, including: (a) providing activated sludge as biological matrix, (b) adding a nitroaromatic-degrading microorganism to the activated sludge, (c) adding nitroaromatic-containing wastewater to the material of step (b) and (d) adding at least one carbon source which is more readily utilizable than the...

20060115888 - Large-scale electroporation plates, systems and methods of use: Described are electroporation plates (2) that comprise a plurality of energizable electroporation wells (4) or chambers arrayed in a solid substrate, wherein at least two of the wells (4) in the plate (2) can be independently energized. Each well (4) contains at least two electroporation electrodes (12, 14) disposed therein,...

20060115889 - Automatic cell cultivation apparatus having a multijoint robot: An automatic cell cultivation apparatus is provided which is capable of conveying the culture container, exchanging the culture medium, and operating other jobs promptly by a robot. The automatic cell cultivation apparatus has a cultivation apparatus main body for cultivating cells in a culture container, and the cultivation apparatus main...

20060115890 - Particle injector for a cell sorter: The invention relates to a particle injector for introducing particles into a carrier flow of a microfluidic system, especially for injecting biological cells into the carrier flow of a cell sorter. The particle injector includes an inlet for receiving the carrier flow, an outlet for discharging the carrier flow including...

20060115891 - Apparatus for minimizing evaporation and/or condensation of samples occurring in tubes of multi-well plate mounted to pcr thermo cycler: The present invention relates to an automated apparatus for effectively preventing evaporation of samples in reaction tubes during DNA amplification (i.e., Polymerase Chain Reaction) and DNA sequencing processes performed in a thermo cycler. The automated apparatus of the present invention comprises a plurality of plungers corresponding to the plurality of...

20060115892 - Incubator: The invention provides an incubator 1 wherein stackers 3 having a plurality of microplate accommodating portions are arranged in a chamber 11, and a microplate transport device 5 is arranged for transporting a microplate 31 within the chamber 11 and moving the microplate 31 into or out of a desired...

20060115893 - Culturing apparatus: A culturing apparatus has a culture vessel having a first elastic seal bonded on its upper face and a culture space formed thereon, and a joint having supplying means for supplying solution such as a medium to this culture vessel and a second elastic seal having microprojection formed at the...

20060115895 - Fish disease models and uses thereof: Generation and use of disease models, for instance in assays to identify and investigate genes and substances involved in disease and disease treatment, identification and use of drug targets. In particular, the present invention relates to generation and use of disease models in fish such as zebrafish. Gradable phenotypes may...

20060115894 - Method of and apparatus for facilitating processes of mammalian cells: A method of facilitating processes of mammalian cells such as at least one of attachment, movement, growth, proliferation and differentiation comprises: supplying liquid comprising biologically compatible polymer to a liquid outlet in the vicinity of a surface and subjecting liquid issuing from the outlet to an electric field to cause...

20060115897 - Compositions and methods modulating variola and vaccinia virus: Antibodies directed to SPICE and VCP which may be used for detection, prevention, and treatment of variola virus or vaccinia virus are provided. Recombinant SPICE and VCP proteins are also provided which are used for enhancing the immune response to variola or vaccinia virus and modulating of complement activation. Methods...

20060115896 - Diagnostic test for west nile virus: The present invention provides a rapid and sensitive method for the detection of a West Nile virus (WNV), Japanese encephalitis virus (JEV), St. Louis encephalitis virus (SLEV) and Dengue virus (DENV) and antibodies directed against thereof involving contacting a biological specimen suspected of being infected with WNV, JE, SLE or...

20060115898 - Cultured hematopoietic stem cells and method for expansion and analysis thereof: Hematopoietic stem cells and methods for ex vivo expansion of hematopoietic stem cells are provided. The methods comprise culturing the cells in a media containing an effective amount insulin-like growth factor (IGF), fibroblast growth factor (FGF), thrombopoietin (TPO), and stem cell factor (SCF), under conditions sufficient for expansion of said...

20060115899 - Methods of generating antigen-specific cd4+cd25+ regulatory t cells, compositions and methods of use: The present invention provides methods for generating mammalian T cell populations comprising antigen-specific CD4+CD25+ regulatory T cells from freshly isolated CD4+CD25− T cells. The method comprises selecting CD4+CD25− T cells from a sample obtained from a mammalian subject; determining the MHC Class II type of the subject; inducing the generation...

20060115900 - Method of treating biological tissue by microwave-irradiation: A method of efficiently removing or fixing donor cells from a native tissue of mammalian origin comprises immersing the tissue in a treating solution, and irradiating the tissue with microwave while maintaining the temperature thereof in the range between 0° C. and 40° C....

20060115901 - Method and media for single cell serum-free culture of cho cells: The present invention relates to methods and media for single cell serum free culture of CHO cells. Generally, the invention relates to a method of culturing CHO cells at a cell density of less than 100 cells/ml in a serum-free cell culture medium. The medium is sufficient to support the...