Chemistry: molecular biology and microbiology inventions 05/06

20060110721 - Apparatus and method for in vitro storage of a cornea: A fixture and method is provided for in vitro storage of a cornea. The fixture includes a platform having a corneal-sceral rim receiving surface, a clamp having a mating surface for the cornel-scleral rim and handles. A locking mechanism is provided to secure a donor cornea between the clamp and...

20060110720 - Method of treating biological tissue for transplantation by applying ultrahigh hydrostatic pressure: Native tissue of mammalian origin are treated for transplantation by applying ultrahigh hydrostatic pressure to the tissue in a liquid medium. The connective tissue is rendered isolatable from the remainder of the tissue including cellular components while keeping intact the structure and biomechanical properties thereof....

20060110722 - Cell mimic platform and method: A platform and method for mimicking the environment within a cell is provided. The platform includes a microfluidic device defining a chamber. At least one hydrogel post is positioned within the chamber of the microfluidic device. Each hydrogel post defines a corresponding pore for receiving a first molecule therein. Second...

20060110725 - Apparatus for and method of purifying nucleic acids by different laser absorption of beads: An apparatus for and method of purifying nucleic acids of cells or viruses are provided. The nucleic acid purification apparatus includes: a cell lysis capillary having a sample inlet through which samples, magnetic beads, and a solid support are introduced; a vibrator attached to the capillary and mixing the samples,...

20060110723 - Multiplexed protein expression and activity assay: A system is for analyzing expression levels and activity of a plurality of proteins is provided. A bio-displayed polypeptide binding component associated with a predetermined marker is used to bind the proteins of interest. The predetermined marker components are then amplified and detected in a high throughput manner....

20060110726 - Polymerase chain reaction assays for monitoring antiviral therapy and making therapeutic decisions in the treatment of acouired immunodeficiency syndrome: The present invention relates to methods of monitoring, via polymerase chain reaction, the clinical progression of human immunodeficiency virus infection and its response to antiretroviral therapy. According to the invention, polymerase chain reaction assays may be used to predict immunological decline and to identify, at an early stage, patients whose...

20060110724 - Quantitative real-time assay for noroviruses and enteroviruses with built in quality control standard: A method is provided for reverse transcription-polymerase chain reaction (RT-PCR) comprising a) amplifying a reverse transcribed cDNA in a mixture comprising Norovirus Genogroup I and Norovirus Genogroup II primers and probes, wherein said Norovirus primers and probes distinguish between Genogroup I and Genogroup II viruses; b) quantifying virus; and c)...

20060110754 - Amplification and separation of nucleic acid sequences using strand displacement amplification and bioelectronic microchip technology: Described and disclosed are devices, methods, and compositions of matter for the multiplex amplification and analysis of nucleic acid sequences in a sample using novel strand displacement amplification technologies in combination with bioelectronic microchip technology. Specifically, a nucleic acid in a sample is amplified to form amplicons, the amplicons are...

20060110759 - Biomarkers for prostate cancer metastasis: The present invention provides genomic markers for determining the predisposition of prostate cancer to become metastasized....

20060110739 - Biosensors for detecting macromolecules and other analytes: The invention generally provides molecular biosensors. In particular, the invention provides molecular biosensors having one or more aptamers. The molecular biosensors are useful in several methods including in the identification and quantification of target molecules....

20060110731 - Cancer-linked gene as target for chemotherapy: Cancer-linked gene sequences, and derived amino acid sequences, are disclosed along with processes for assaying potential antitumor agents based on their modulation of the expression of these cancer-linked genes. Also disclosed are antibodies that react with the disclosed polypeptides and methods of using the antibodies to treat cancerous conditions, such...

20060110742 - Carrier tests for polycystic kidney disease in the cat: The present invention provides compositions and methods for detecting mutations associated with polycystic kidney disease in the cat....

20060110750 - Cleavage of nucleic acids: The present invention relates to means for the detection and characterization of nucleic acid sequences, as well as variations in nucleic acid sequences. The present invention also relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a...

20060110733 - Coded nucleic acid carriers: The present invention relates generally to coded solid or semi-solid nucleic acid carriers for use in multiplexing solid phase nucleic acid-based reactions. The use of coded carriers facilities multiplexing due to the ability to deconvolute multiple nucleic acid-based events and to correlate those to particular experiments. The present invention further...

20060110770 - Deoxyribonuclease, gene encoding same and use thereof: This invention provides a novel acid DNase (DLAD) which is an endonuclease capable of cleaving DNA independently from divalent cations, under acidic conditions, which retains its activity in acidic to even neutral pH range, and which is not inhibited by G-actin. This invention also provides a DNA encoding the enzyme,...

20060110765 - Detection of nucleic acid variation by cleavage-amplification (cleavamp) method: Methods and compositions for detecting nucleic acid polymorphisms are provided....

20060110734 - Diagnosis of shwachman-diamond syndrome: The SBDS gene has been identified as the site of mutations associated with SDS. Methods are provided for determining whether a subject is suffering from SDS....

20060110743 - Drug evolution: drug design at hot spots: A new method of designing and generating compounds having an increased probability of being drugs, drug candidates, or biologically active compounds, in particular having a therapeutic utility, is disclosed. The method consists of identifying a group of bioactive compounds, preferably of diverse therapeutic uses or biological activities and built on...

20060110767 - Genotyping assay to predict cytochrome p4503a5 exression: Genetic polymorphisms responsible or associated with altered expression of cytochrome P450 CYP3A5 enzyme are described. Single nucleotide polymorphisms are provided. Methods for identifying subjects having a low or high drug metabolizing phenotype associated with CYP3A5 expression are provided. Assays, kits and methods for determining and assaying the CYP3A5 genotype and...

20060110736 - Hiv vaccine formulations: Provided herein are HIV vaccines comprising HIV polypeptide-encoding DNA adsorbed to PLG and/or HIV proteins. Also provided are methods of using these vaccines to generate immune responses in a subject....

20060110737 - Human tumor-associated gene ct120 on chromosome 17p 13.3 region and the protein encoded by it: The invention related to a novel human tumor associated polypeptide CT120, and the encoding polynucleotide, and to the method for producing the polypeptide by recombinant technology, also to the method for diagnosing and treating diseases such as carcinoma using the polypeptide. The invention also related to antagonists against the polypeptide...

20060110761 - Identification of a gene causing the most common form of bardet-biedl syndrome and uses thereof: The present invention relates to the identification of a gene, mutated at the most common locus now designated BBS1, that is involved in the genetic disease Bardet Biedl Syndrome (BBS), which is characterized by such diverse symptoms as obesity, diabetes, hypogonadism, mental retardation, renal cancer and other renal abnormalities, retinopathy...

20060110756 - Large-scale parallelized dna sequencing: We provide a DNA sequencing method and a sequencing system where large numbers of sequence reads can be obtained in parallel by running traditional electrophoresis in a special format. Parallelization is obtained either through a 3-dimensional gel-cube or through bundled capillary tubes including fiber-optic tubes or other types of micro...

20060110764 - Large-scale parallelized dna sequencing: We provide a DNA sequencing method and a sequencing system where large numbers of sequence reads can be obtained in parallel by running traditional electrophoresis in a special format. Parallelization is obtained either through a 3-dimensional gel-cube or through bundled capillary tubes including fiber-optic tubes or other types of micro...

20060110751 - Method and kit for detecting a risk of essential arterial hypertension: Genes, SNP markers and haplotypes of susceptibility or predisposition to hypertension (HT) are disclosed. Methods for diagnosis, prediction of clinical course and efficacy of treatments for HT using polymorphisms in the HT risk genes are also disclosed. The genes, gene products and agents of the invention are also useful for...

20060110745 - Method for amplifying nucleic acids: The invention provides a sequence specific method for amplifying nucleic acids. More particularly, the invention provides a method for amplifying nucleic acid sequences which enables such sequences to be detected with high precision, rapidity and high specificity as compared to conventional methods. The present invention also provides a simple method...

20060110768 - Method for determining genotoxicity: Methods and reagents for determining the genotoxicity of a compound based on genomic responses to contact therewith are provided....

20060110732 - Method for the identification of ligands: The present invention relates generally to a method of identifying ligands that modulate protein-protein interactions. More particularly, the present invention relates to methods of determining agonists or antagonists of a co-regulator dependent target molecule based on the ability to modify the stability of the target molecule in a tissue-selective manner....

20060110757 - Method of designing probe from polynucleotide group comprising plurality of polynucleotides: There is provided a method of designing a probe from a polynucleotide group comprising a plurality of polynucleotides, the method including: forming a polynucleotide group by selecting polynucleotides having a certain sequence; producing polynucleotide fragments having a certain length from each of the polynucleotides of the polynucleotide group and obtaining...

20060110766 - Method of determining a cellular response to a biological agent: A method of determining a cellular response to a biological agent can be preformed in the presence of gene silencing. Such a method can include the use of siRNA in order to silence various genes in the cell in order to monitor the cellular response to the biological agent when...

20060110729 - Method of dna testing for mycobacterium paratuberculosis strains: The present invention relates to the discovery of a DNA sequence in sheep types of M. paratuberculosis that differs from the homologous sequence in cattle types of M. paratuberculosis. The invention also provides a nucleic acid amplification technique based on these differences that can be used to distinguish strains of...

20060110730 - Methods and compositions for regulation and manipulation of steroidogenesis: The steroidogenic acute regulatory protein (StAR), a mitochondrial protein required for stress responses, reproduction, and sexual differentiation of male fetuses is shown to exert its activity transiently at the outer mitochondrial membrane (OMM) rather than in its final resting place in the matrix, and that its OMM residency time and...

20060110748 - Methods for detection of a nucleic acid by sequential amplification: The invention relates to nucleic acid, flap-mediated, sequential amplification methods which permit detection of a nucleic acid target in a nucleic acid sample. The invention provides for nucleic acid synthesis dependent, flap-mediated amplification methods for sequentially producing detectable, released flaps to detect a target nucleic acid. The invention provides for...

20060110753 - Methods to diagnose and treat lung cancer: The present invention provides compositions and methods for aiding in the diagnoses of the neoplastic condition of a lung cell, and methods of screening for a potential therapeutic agents for the reversal of the neoplastic condition. Also provided are therapeutic compositions and methods to inhibit the growth of neoplastic lung...

20060110760 - Microarray using laminar flow and method of preparing the same: A microarray including hydrogel and a plurality of probes which are immobilized in discrete regions of the hydrogel, and a method of preparing the same are provided. When using the microarray and method, a solid substrate is not required and many biomolecules can be immobilized in a small volume, thereby...

20060110727 - Modified nucleic acid probes: Oligonucleotide analogue arrays attached to solid substrates and methods related to the use thereof are provided. The oligonucleotide analogues hybridize to nucleic acids with either higher or lower specificity than corresponding unmodified oligonucleotides. Target nucleic acids which comprise nucleotide analogues are bound to oligonucleotide and oligonucleotide analogue arrays....

20060110741 - Nucleic acid methylation detection process using an internal reference sample: There is disclosed a process for detection of DNA methylation at CpG sites using nucleic acid arrays and preferably microarrays. Specifically, there is disclosed a process for directly generating a reference sample from the sample to be tested and detecting methylation at large numbers of CpG island sites simultaneously. More...

20060110763 - Nucleic acid preparation: The present invention is directed to methods, devices and computer programs for preparing nucleic acids from a template nucleic acid by subjecting a sample to thermocycles. After a first number of thermocycles, a partial amount of the reaction mixture is being subjected to a second number of thermocycles. This two...

20060110738 - Polarization-enhanced detector with gold nanorods for detecting nanoscale rotational motion and method therefor: A nanoscale motion detector attaches a gold nanorod (30) to the rotating arm (26) of a molecular structure (10) to cause the nanoparticle to rotate. The molecular structure is an F1-ATPase enzyme. The gold nanorod is exposed to a light source. The long axis of the gold nanorod scatters red...

20060110752 - Polymorphism detection: The present invention generally provides a rapid efficient method for analyzing polymorphic or biallelic markers, and arrays for carrying out these analyses. In general, the methods of the present invention employ arrays of oligonucleotide probes that are complementary to target nucleic acids which correspond to the marker sequences of an...

20060110762 - Porcine reproductive and respiratory syndrome virus receptor components and uses thereof: This invention relates to host cellular receptor components for Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) and methods of their use in diagnosis, prevention, control, and treatment of PRRSV disease in animals....

20060110749 - Primer composition and method of using the same in the detection of shigella sonnei: The invention provides a new primer composition for detecting the presence of Shigella sonnei and a method of using the same. The primer composition and method have high specificity and sensitivity on the detection of Shigella sonnei. The invention also provides a method for extracting the nucleic acids of microorganisms...

20060110744 - Probe design methods and microarrays for comparative genomic hybridization and location analysis: Methods and systems for identifying and selecting nucleic acid probes for detecting a target with a nucleic acid probe array or comparative genome hybridization microarray, comprising selecting a plurality of potential target sequences, generating a plurality of candidate probes from the target sequences, filtering the plurality of candidate probes by...

20060110747 - Process for improved protein expression by strain engineering: This invention is a process for improving the production levels of recombinant proteins or peptides or improving the level of active recombinant proteins or peptides expressed in host cells. The invention is a process of comparing two genetic profiles of a cell that expresses a recombinant protein and modifying the...

20060110769 - Regulation of novel human prolyl 4-hydroxylases: Reagents that regulate human prolyl 4-hydroxylase and reagents which bind to human prolyl 4-hydroxylase gene products can play a role in preventing, ameliorating, or correcting dysfunctions or diseases including, but not limited to cardiovascular disorders, cancer, inflammatory diseases, fibrotic disorders, anemia, and CNS disorders, such as stroke....

20060110728 - Supported double layer structure for displaying a nucleic acid associated with a protein: The invention concerns novel biosensors, in particular a support for displaying nucleic acids and for detecting both the presence of nucleic acids in a sample and the linkage between proteins and nucleic acids, as well as the linkage between a ligand and a protein linked to a nucleic acid....

20060110758 - Synthetic peptide targeting critical sites on the sars-associated coronavirus spike protein responsible for viral infection and method of use thereof: The present invention provides methods for locating critical portions or sites on the spike protein (S protein) of SARS-associated coronavirus (SARS-CoV) responsible for the viral infection that causes Severe Acute Respiratory Syndrome (SARS). The present invention also provides new synthetic peptides targeting such critical portions or sites of the S...

20060110746 - Treatment of cancer using tlr3 agonists: The present invention relates generally to the fields of genetics and medicine. More specifically, the present invention relates to improved methods of treating cancers using a TLR3 agonist, by assessing the expression of a TLR3 receptor by cancer cells....

20060110735 - Use of molecular markers for the preclinical and clinical profiling of inhibitors of enzymes having histone deacetylase activity: The present invention relates to the use of molecular markers and related signaling mechanisms for the preclinical and clinical profiling of inhibitors of enzymes having histone deacetylase activity. The invention also relates to the use of such markers as diagnostic and/or prognostic tools for the treatment of tumor patients with...

20060110740 - Use of sendai virus as a human parainfluenza vaccine: Immunogenic compositions to protect against human parainfluenza (hPIV) virus infection are provided. The compositions comprise an unmodified Sendai virus that can be safely administered to human subjects, particularly young children, to protect against symptoms and health risks associated with hPIV infection....

20060110755 - Yeast-based therapeutic for chronic hepatitis c infection: Disclosed are compositions, including vaccines, and methods for vaccinating an animal against hepatitis C virus (HCV) and for treating or preventing hepatitis C viral infection in an animal. The invention includes a variety of novel HCV fusion proteins that can be used directly as a vaccine or in conjunction with...

20060110772 - Human and murine cytokine polypeptides: This invention relates to IMX7189 cytokines and to new members of the human cytokine polypeptide family, methods of making such polypeptides, and to methods of using them to treat conditions and diseases involving proliferation and/or differentiation of cells from pluripotent stem cell precursors, and to identify compounds that alter cytokine...

20060110773 - Marker for inflammatory conditions: Use of pregnancy-associated plasma protein-A as a marker for inflammatory conditions, and in particular, for acute coronary syndromes is described....

20060110775 - Method of detecting native probnp: The present invention relates to antibodies specifically binding to native proBNP, a method for specific detection of native proBNP, a method of correlating the level of native proBNP to the diagnosis of heart failure, a kit for detection of native proBNP and to a hybridoma cell line producing an antibody...

20060110776 - Method of detecting native probnp: The present invention relates to antibodies specifically binding to native proBNP, a method for specific detection of native proBNP, a method of correlating the level of native proBNP to the diagnosis of heart failure, a kit for detection of native proBNP and to a hybridoma cell line producing an antibody...

20060110774 - Methods for detecting sepsis: Compositions and methods for detecting sepsis by contacting a subject-derived sample with a ligand that binds to GRK and determining the concentration of GRK in the sample. An increase in the concentration of GRK compared to that of a normal, healthy control sample indicates that the subject from which the...

20060110771 - Proteins capable of binding to female sex hormones and process for producing the same: The present invention provides a protein capable of binding to a female sex hormone, which protein is supplemented with useful properties for measuring, quantifying and concentrating female sex hormones, such as high sensitivity, low cross-reactivity, unlikelihood of being influenced by interfering substances, and unlikelihood of being influenced by solvents. Specifically,...

20060110777 - Structured peptide scaffold for displaying turn libraries on phage: The invention is directed to a model system for structure-activity analysis of peptide or protein molecules involved in important biological processes. Provided by the invention are combinatorial peptide libraries comprising disulfide-constrained cyclic peptides with sequences favorable for energy stabilized conformations. One aspect of the invention is directed to cyclic peptide...

20060110778 - High-throughput screen for identifying selective persistent sodium channels channel blockers: A method for identifying a selective persistent Na+ channel blocker by measuring the ability of the blocker to reduce or inhibit a persistent Na+ current to a greater degree than a transient Na+ current. Aspects of the present method provide Na+ depletion/repletion methods for identifying a selective blocker of a...

20060110779 - Method for detecting a lectin-like substance: The present invention provides a method for detecting lectin-like substances, which comprises the steps of (a) providing a first carbohydrate molecule and a test target, wherein the first carbohydrate molecule specifically binds to the lectin-like substance to be detected; (b) contacting the test target with the first carbohydrate molecule so...

20060110780 - Plasma c5a levels as an indicator of asthma severity: This invention provides the first blood test which characterizes asthma severity. More specifically, the present invention provides methods of determining the severity of acute asthma in a patient by determining the levels of C5a or C5a-desArg in the patient's blood, plasma or serum....

20060110781 - Cholesterol detection reagent: An object of the present invention is to provide a novel cholesterol detection reagent comprising a substance which can specifically binds to cholesterol to detect it, and a method for detecting cholesterol using the reagent. The present invention provides a cholesterol detection reagent comprising a polyethylene glycol cholesteryl ether which...

20060110782 - Compositions and methods for modification of biomolecules: The present invention provides modified cycloalkyne compounds; and method of use of such compounds in modifying biomolecules. The present invention features a cycloaddition reaction that can be carried out under physiological conditions. In general, the invention involves reacting a modified cycloalkyne with an azide moiety on a target biomolecule, generating...

20060110783 - Diagnostics and therapeutics for diseases associated with human phosphodiesterase 10a (pde10a): The invention provides a human PDE10A which is associated with the disorders of the peripheral and central nervous system (e.g. Alzheimer), cardiovascular diseases, cancer (e.g. thyroid, oesophagus, stomach, liver, ovary, kidney), gastroenterological diseases (e.g. liver cirrhosis), endocrinological diseases and urological diseases (e.g. benign prostate hyperplasia). The invention also provides assays...

20060110785 - Methods to differentiate protein conformers: The invention is directed to methods to distinguish among different protein conformers of the same protein such as proteins which form amyloid deposits. Using the methods of the invention, one or more protein conformers in a sample can be detected, differentiated, and quantitated. An example of a protein which is...

20060110784 - Site-specific labeling of proteins for nmr studies: Methods of producing and/or analyzing spectroscopically labeled proteins, e.g., proteins site-specifically labeled with NMR active isotopes, spin-labels, chelators for paramagnetic metals, and the like, are provided. The labeled proteins are produced in translation systems including orthogonal aminoacyl tRNA synthetase/tRNA pairs. Methods for assigning NMR resonances, e.g., methods using isotopically labeled...

20060110786 - Compositions and methods for detecting and quantifying cox-2 activity and 2-arachidonylgycerol metabolites: The present invention provides methods, compositions and kits for discriminating between COX-1 and COX-2 activity. In particular, the prevent invention provides for the detection and/or measurement of COX-2 activity in subjects, samples thereof, and in laboratory tests. The present invention discloses that 2-arachidonylglycerol is a COX-2 selective substrate which is...

20060110787 - Use of fluorine-18-labeled fluoroquinolone antibiotics for diagnosing and monitoring bacterial infection: The invention provides methods of diagnosing and imaging bacterial infections in mammals using 18F-labeled fluoroquinolone antibiotics and detecting the fluorine-18. Fluorine-18 can be detected and imaged by positron emission tomography. The invention also provides methods of detecting bacteria in other settings, and of treating a bacterial infection and monitoring treatment...

20060110788 - Feeding buffers, systems, and methods for in vitro synthesis of biomolecules: Compositions, methods and kits for in vitro systems for synthesis of biomolecules such as polypeptides, are provided herein. Cell extracts that provide enhanced yields of soluble proteins using in vitro protein synthesis methods are provided. The invention also includes methods for producing high yields of proteins by the addition of...

20060110798 - Cells expressing recombinase useful for adenovirus vector production and control of gene expression: Embodiments of the invention provide cells that stably express an integrase family recombinase, for example Cre or FLP. Examples of production of such cells, and uses of such cells, are provided. In some embodiments, a cell may additionally express one or more adenovirus E1 proteins. In some embodiments, the cell...

20060110790 - Expression system for large functional proteins: The present invention provides a system and method for expressing functional ABC (ATP-binding cassette) proteins, from the ABCA subfamily, in a host cell. A system comprises two or more expression vectors each comprising a nucleic acid molecule encoding one or more domains of an ABC transporter gene and a means...

20060110791 - Heterologus expression of trypanothione reductase from leishmania donovani in a prokaryotic system: The present invention relates to a process for heterologous expression and large-scale production of functionally active enzyme trypanothione reductase of Leishmania donovani in prokaryotic system....

20060110796 - In vivo site-specific incorporation of n-acetyl-galactosamine amino acids in eubacteria: Methods and compositions for making glycoproteins, both in vitro and in vivo, are provided. One method involves incorporating an unnatural amino acid having a N-acetylgalactosamine moiety into a protein; optionally, the N-acetylgalactosamine-containing unnatural amino acid can be further modified with additional sugars....

20060110793 - Methods and compositions for increasing longevity and protein yield from a cell culture: Disclosed herein are compositions and methods for increasing the longevity of a cell culture and permitting the increased production of proteins, preferably recombinant proteins, such as antibodies, peptides, enzymes, growth factors, interleukins, interferons, hormones, and vaccines. By transfecting cells in culture with an apoptosis-inhibiting gene or vector, cells in culture...

20060110789 - Methods to mediate polyketide synthase module effectiveness: Linking sequence which modulate cross-talk between modules of Type I polyketide synthases have been identified. Thus, arbitrarily chosen modules can be mixed and matched by supplying the appropriate linkers to obtain desired polyketide synthases and new polyketides. The modules are provided suitable linkers so that the polyketide chain is passed...

20060110799 - Mitogen-activated protein kinase kinase mek6 and methods of use thereof: Compositions and methods are provided for potentiating the activity of the mitogen-activated protein kinase p38. In particular the mitogen-activated protein kinase kinase MEK6, and variants thereof that stimulate phosphorylation of p38 are provided. Such compounds may be used, for example, for therapy of diseases associated with the p38 cascade and...

20060110794 - Peptides from the e2 proteins of human papillomaviruses 16 and 18 for detecting and/or diagnosing cervical and other human papillomavirus associated cancers: An isolated protein sequence or peptide from the E2, E6 or E7 early coding region of human papillomavirus (HPV) that is soluble in an aqueous medium, and characterized by a relative paucity of tryptophan, methionine and cysteine residues, and a relative abundance of glycine and asparagine residues. Also disclosed are...

20060110797 - Polypeptides having xyloglucanase activity and nucleic acids encoding same: The present invention relates to isolated polypeptides having Family 74 xyloglucanase activity and isolated nucleic acid sequences encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences as well as methods for producing and using the polypeptides....

20060110792 - Potassium channels, nucleotide sequences encoding them, and methods of using same: This invention relates generally to a new family of potassium channels. More particularly, the present invention relates to the cloning and characterization of a family of distinct trans-membrane potassium ion channels, characterization of such channels, newly identified polynucleotide sequences, polypeptides encoded by such sequences, expression vectors capable of heterologous expression...

20060110795 - Protein secretion in eukaryotic cells: The present invention relates to the use of a glucosidase II mutation to increase protein secretion in eukaryotic cells. The present invention relates further to the use of eukaryotic cells, comprising a mutant glucosidase II gene, possibly in combination with the expression of a recombinant α-1,2-mannosidase gene and/or a recombinant...

20060110800 - Testis-specific receptor: Novel receptor polypeptides, polynucleotides encoding the polypeptides, and related compositions and methods are disclosed. The polypeptides comprise an extracellular domain of a cell-surface receptor that is expressed in testis cells. The polypeptides may be used within methods for detecting ligands that promote the proliferation and/or differentiation of testis cells, and...

20060110801 - Receptor for b. anthracis toxin: The present invention relates to mammalian anthrax toxin receptor polypeptides and polynucleotides encoding same as well as related polypeptides and polynucleotides, vectors containing the polynucleotides and polypeptides, host cells containing related polynucleotide molecules, and cells displaying no anthrax toxin receptor on an exterior surface of the cells-minus cell lines and...

20060110802 - Cytokine designated 4-1bb ligand and human receptor that binds thereto: Novel 4-1BB ligand (4-1BB-L) polypeptides and a human cell surface receptor designated 4-1BB that binds 4-1BB-L are provided. Isolated 4-1BB-L-encoding and human 4-1BB-encoding DNA sequences, recombinant expression vectors comprising the isolated DNA sequences, and host cells transformed with the recombinant expression vectors are disclosed, along with methods for producing the...

20060110803 - Antigenic peptides of sars coronavirus and uses thereof: The present invention pertains to antigenic peptides of SARS-CoV and their use in diagnostic test methods and in the treatment of condition resulting from SRAS-CoV. Furthermore, this invention provides antibodies capable of specifically recognizing the peptides of the invention. The antibodies can also advantageously be used in diagnostic test methods...

20060110804 - Method of treating an organ for transplantation: The present invention provides a method of treating an organ for transplantation, in which an organ graft is cold-preserved with an organ preservation solution for a given period of time and reperfused with a solution containing an intracellular accessibility, lipophilic small-molecular antioxidant (e.g., bilirubin) to wash the organ graft before...

20060110805 - Microbial production of xylitol via hexose phosphate and pentose phosphate intermediate: The invention provides methods and compositions for production of xylitol, xylose, or combinations thereof from simple sugars from biosynthetic pathways that utilize D-fructose-6-phosphate and D-xylulose-5-phosphate as pathway intermediates....

20060110806 - Docosahexaenoic acid producing strains of yarrowia lipolytica: An engineered strain of the oleaginous yeast Yarrowia lipolytica capable of producing greater than 5.6% docosahexaenoic acid acid (DHA, an w-3 polyunsaturated fatty acid) in the total oil fraction is described. This strain comprises various chimeric genes expressing heterologous desaturases, elongases and acyltransferases and optionally comprises various native desaturase and...

20060110807 - Process for the preparation of phenolic carboxylic acid derivaties by enzymatic catalysis: The present invention relates to an improved process for the preparation of phenolic carboxylic acid derivatives catalysed by biocatalytic esterification, transesterification or amidation of a corresponding lower alkyl ester. Biocatalysis is performed in the presence of suitable enzymes, e.g. hydrolases, especially esterases, amidases, lipases and proteases....

20060110808 - Methods for preserving and/or storing cellshaving a nitrilase or nitrile hydratase avtivity: The invention relates to a method for preserving and/or storing microorganisms which exhibit at least one nitrile hydratase or nitrilase enzyme activity, with the preservation and/or storage being effected in an aqueous medium which comprises at least one aldehyde, with the total aldehyde concentration being in a range from 0.1...

20060110809 - Methods for production of xylitol in microorganisms: The invention provides biosynthetic routes to xylitol production that do not require pure D-xylose for synthesis and that can utilize inexpensive substrates such as hemicellulose hydrolysates....

20060110810 - Methods and materials for the synthesis of organic products: This invention provides biocatalysts that are recombinant yeast cells comprising recombinant expression vectors encoding heterologous lactate dehydrogenase genes for producing lactate....

20060110811 - Novel candida tropicalis cj-fid(kctc 10457bp) and manufacturing method of xylitol thereby: Provided are a novel Candida tropicalis CJ-FID (KCTC 10457BP) derived from honey and a method for producing xylitol using the same. The method includes culturing the Candida tropicalis CJ-FID itself or an alginate-immobilized Candida tropicalis CJ-FID in a fermentation medium containing high concentration xylose and sucrose to obtain xylitol-containing culture,...

20060110812 - Methods and compositions for simultaneous saccharification and fermentation: The invention provides compositions and methods for the synergistic degradation of oligosaccharides by endoglucanases. The invention further provides recombinant host cells containing one or more genes encoding endoglucanses which are capable of the synergistic degradation of oligosaccharides. Preferred host cells of the invention are ethanologenic and capable of carrying out...

20060110813 - Process for producing l-glutamic acid: L-glutamic acid is produced by culturing in a liquid medium containing L-glutamic acid at a saturation concentration and the carbon source a microorganism that can metabolize a carbon source at a specific pH, and wherein said microorganism has an ability to accumulate L-glutamic acid in said medium in an amount...

20060110814 - Structural-based inhibitors of the glutathione binding site in aldose reductase, methods of screening therefor and methods of use: Provided herein is a crystallized ternary structure of aldose reductase (AR) bound to NADPH and γ-glutamyl-S-(1,2-dicarboxyethyl)cysteinylglycine (DCEG). Also provided are specific inhibitors of glutathione-aldehyde binding to aldose reductase which are designed via at least computer modeling of the ternary AR:NADPH:DCEG structure and methods of designing and of screening the inhibitors...

20060110815 - Personal care composition containing ozone-stressed yeast lysates: The present invention relates to an ozone-stressed yeast lysate, and a personal care composition including an ozone-stressed yeast lysate and at least one preservative selected from the group consisting of alcohols, glycols, parabens, hydantoins, quaternary nitrogen-containing compounds, isothiazolinones, aldehyde-releasing agents, and halogenated compounds. The present invention also relates to a...

20060110816 - Method of concentrating and removing harmful substance using double-stranded dna and adsorbent and apparatus therefor: There is provided a method of removing harmful substance characterized by comprising contacting a harmful substance-containing liquid to be treated with a double-stranded DNA, adsorbing the harmful substance onto the double-stranded DNA through intercalation, and separating it. The method is particularly useful for removing dioxins, and can be carried out...

20060110817 - Capturing apparatus and method of minute objects: A capturing apparatus that captures plural minute objects that float on a fluid, includes a capturing part having plural attraction ports that can attract each object, and an induction part that creates a flow in the fluid used to induce the plural objects to the attraction ports....

20060110818 - Aerosol into liquid collector for depositing particles from a large volume of gas into a small volume of liquid: An aerosol-into-liquid collector (ALC) for collecting gas-borne particles from a large volume of gas such as air into a small volume of liquid is described. The ALC uses a linear quadrupole to concentrate particles flowing in a gas and to help direct these concentrated particles toward a small volume of...

20060110819 - Apparatus and method for expression and capture of biomolecules and complexes on adsorbent surfaces: The present invention relates to the fields of molecular biology, combinatorial chemistry and biochemistry. Particularly, the present invention describes apparatus and methods for the detection and isolation of binding partners and activity modulators for biomolecules. The apparatus described allows for expression, capture and analysis of one or more biomolecules in...

20060110820 - Capturing apparatus and method of minute objects: A capturing apparatus that captures plural minute objects that float on a fluid, includes a capturing part that includes plural attraction ports, each of which can attract each object, wherein attractions by the plural attraction ports are independent of each other....

20060110821 - Devices for motile sperm separation: Device (10, 110) for separating motile sperm from a sample (100, 200), comprises a vessel (15, 115) having an inlet port (20, 120), an outlet port (35, 135) which is initially closed, a medium (40, 140) into which motile sperm in the sample (100, 200) can migrate into the vessel...

20060110822 - Perfusion bioreactors for culturing cells: A bioreactor system comprising a multi-well platform including an array of bioreactor units. The bioreactor system comprises a perfusion unit and a fluid source unit fluidly interconnected by a pumping unit. The perfusion unit comprises a multi-well plate including a plurality of main chambers configured to house or contain a...

20060110823 - Transgenic mice containing glucocorticoid-induced receptor gene disruptions: The present invention relates to transgenic animals, as well as compositions and methods relating to the characterization of gene function. Specifically, the present invention provides transgenic mice comprising mutations in a glucocorticoid-induced receptor gene. Such transgenic mice are useful as models for disease and for identifying agents that modulate gene...

20060110824 - Method for culturing avian primordial germ cells (pgcs) for a long period and preparing a medium for culturing avian pgcs for a long period: The present invention is a method for culturing avian PGCs comprising providing fibroblast cells, culturing fibroblast cells in a basal medium, collecting a conditioned medium, providing avain gonadal cells with PGCs from sex glands, and growing the PGCs in the conditioned medium supplemented with growth factors, wherein the conditioned medium...

20060110825 - Process for the preparation of stem cells from human muscle tissue and adipose tissue, and stem cell obtainable by this process: The invention relates to a process for the preparation of human stem cells from muscle tissue or adipose tissue. The process provides for the incubation of cells obtained from a sample of muscle tissue or adipose tissue in a medium comprising BSA, bFGF, EGF, VEGF, LIF, heparin and usual inorganic...

20060110826 - Methods for perfusion and plating of primary hepatocytes and a medium therefore: The present invention provides methods for culturing primary hepatocytes with improved long term function and improved viability, by plating the hepatocytes in the presence of an anti-oxidants) as well as an agent(s) which is a functional inhibitor of enzymes that generate reactive oxygen and reactive nitrogen species. One preferred embodiment...

20060110827 - Red and near infrared flourescent phyotochrome: This invention provides novel fluorescent adducts that emit in the far red and/or near infrared. In certain embodiments, the adducts comprise a mutant apoprotein and a bilin....

20060110828 - Compositions and methods for selection of a pure population of cells from a mixed population: Disclosed are methods and compositions for deriving a pure population of differentiated cells from a stem cell. The method comprises transforming a stem cell with a gene construct containing a selectable marker under the control of a tissue- or cell-type-specific regulatory element, such that the gene construct integrates at a...

20060110829 - Apparatus and system having dry gene silencing pools: A reverse transfection apparatus can be used for introducing siRNA into a cell to effect gene silencing. Such an apparatus can include a well plate having a well configured for transfecting cells. The well can include a substantially dry gene silencing composition that has at least two siRNAs which silences...

20060110830 - De-differentiation and re-differentiation of somatic cells and production of cells for cell therapies: The invention provides a method for effecting the de-differentiation of a somatic cell by culturing the cell in the absence of growth factors, cytokines, or other differentiation-inducing agents, and introducing components of cytoplasm of plutipotent cells into the somatic cell and allowing the cell to de-differentiate. The method can be...

20060105317 - Method and solutions for cryopreserving oocytes, especially fresh human oocytes: In a method for cryopreserving fresh human oocytes, freezing and thawing solutions are used, which solutions include 1,2 propanediol (PROH) and sucrose at a concentration of at least 0.3M. The oocytes are exposed for 13-15 minutes to a solution including 1.5M PROH and 0.3M sucrose before starting the freezing process....

20060105318 - Compositions and methods for non-targeted activation of endogenous genes: The present invention is directed generally to activating gene expression or causing over-expression of a gene by recombination methods in situ. The invention also is directed generally to methods for expressing an endogenous gene in a cell at levels higher than those normally found in the cell. In one embodiment...

20060105320 - Electrical conductors and devices from prion-like proteins: The present invention provides novel polypeptides comprising a prion-aggregation domain and a second domain; novel polynucleotides encoding such polypeptides; host cells transformed or transfected with such polynucleotides; novel fibrils with specific functionalities and unusually high chemical and thermal stability; and methods of making and using the foregoing....

20060105322 - Intracellular metabolic flux analysis method using substrate labeled with isotope: A method for analyzing an intracellular metabolic flux comprising determining the intracellular metabolic flux from analytical values of cells cultured in a medium containing an isotope-labeled substrate as a carbon source on the basis of an intracellular metabolic flux model constructed for the intracellular metabolic flux to be analyzed, wherein...

20060105319 - Method for converting venous blood values to arterial blood values, system for utilising said method and devices for such system: The invention relates to methods of converting venous blood values to arterial blood values. This has the advantage that arterial blood samples need not be taken, and the disadvantages compared to venous blood samples when taking arterial blood samples are eliminated. The method is basically based on three steps, namely...

20060105321 - Methods for evaluating cell membrane properties: Methods for measuring cell membrane property are disclosed. A computer readable medium includes instructions for deriving from transcellular impedance a measurement of a cell membrane property and provides the stability and error of the measurement. The derivation from the transcellular impedance includes the real and imaginary components of the transcellular...

20060105325 - Adsorbent with enhanced protein binding capacity and selectivity: The invention discloses an adsorbent composition in which each ligand has a cluster of functional groups. This adsorbent composition is designed to enhace binding affinity and specificity for biomolecules. The invention also discloses a method for adsorbing a biomolecule onto the disclosed adsorbent composition. A biomolecule can be adsorbed to...

20060105327 - Inhibition of the src kinase family pathway as a method of treating hbv infection and hepatocellular carcinoma: The present invention relates to therapeutic protocols and pharmaceutical compositions designed to target HBx mediated activation of Src kinase, members of the Src tyrosine kinase family and components of the Src kinase family signal transduction pathways for the treatment of HBV infection and related disorders and diseases, such as HCC....

20060105326 - Method for isolating proteins or protein and nucleic acid associations, or particle and protein complexes, reagent and uses: The magnetic colloidal particles comprise a core and an envelope in which the core is magnetic and is coated with at least one polymer comprising functional groups X chosen from amine, hydroxyl, thiol, aldehyde, ester, anhydride, acid chloride, carbonate, carbamate, isocyanate and isothiocyanate groups, or mixtures thereof, at least one...

20060105324 - Methods and compositions for predicting the outcome of cervical intra-epithelial neoplasia: The present invention relates to methods and kits for predicting the outcome of cervical intra-epithelial neoplasia and methods for treating a cervical intra-epithelial neoplasia. More particularly, the methods comprise the determination of the presence of the HLA-DRB1*13 allele and the absence of a high risk human papillomavirus (HPV)....

20060105323 - Multi-reporter gene model for toxicological screening: A system for the detection of gene activation events is provided which comprises a nucleic acid construct encoding a protein of the lipocalin protein family and a peptide tag in which the expression of the construct in a cell or in the cells of a transgenic animal demonstrates the activation...

20060105386 - 17867, a novel human aminopeptidase: The present invention relates to a newly identified human aminopeptidase. The invention also relates to polynucleotides encoding the aminopeptidase. The invention further relates to methods using the aminopeptidase polypeptides and polynucleotides as a target for diagnosis and treatment in aminopeptidase-related disorders. The invention further relates to drug-screening methods using the...

20060105364 - Best's macular dystrophy gene: Novel human and mouse DNA sequences that encode the gene CG1CE, which, when mutated, is responsible for Best's macular dystrophy, are provided. Provided are genomic CG1CE DNA as well as cDNA that encodes the CG1CE protein. Also provided is CG1CE protein encoded by the novel DNA sequences. Methods of expressing...

20060105383 - Biopolymer detecting method and biochip: The present invention relates to biopolymer detection utilizing antigen-antibody reaction, intended to improve the S/N ratio, to increase the detection sensitivity, and to shorten the detection time. According to the present invention, target biopolymers labeled with a fluorescent material and beads, onto the surface of which probe biopolymers and beads-ID...

20060105375 - Bioretrosynthetic methods for preparing products and compositions related thereto: Disclosed herein are methods of producing molecules in a natural system and methods for identifying metabolic pathways. Also, disclosed herein are methods and compositions for constructing biosynthetic pathways in whole cells and cell-extracts to produce molecules of interest....

20060105373 - Charge perturbation detection system for dna and other molecules: Methods and apparatus for direct detection of chemical reactions are provided. In a preferred embodiment, electric charge perturbations of the local environment during enzyme-catalyzed reactions are sensed by an electrode system with an immobilized target molecule. The target molecule is preferably DNA. The charge perturbation caused by the polymerase reaction...

20060105365 - Chimeric gb virus b (gbv-b): The present invention relates generally to the fields of biochemistry, molecular biology, and virology. More particularly, it relates to the production and use of GB virus B (GBV-B)/HCV chimeras. The invention involves nucleic acid constructs and compositions encoding GBV-B/HCV chimera. The chimeric viruses may be employed to study GBV-B and...

20060105335 - Color coated layer-by-layer microcapsules serving as combinatory analysis libraries and as specific optical sensors: Monodisperse colloids were coated with polyelectrolytes using the layer-by-layer method. The template cores can remain in the interior or be dissolved away. Various fluorescent dyes are covalently bonded, in defined quantity, to the polyelectrolytes. The quantity of dye is controlled by varying the label content or by coprecipitating unlabeled polymers....

20060105372 - Compositions and methods for purifying nucleic acids from stabilization reagents: The invention features reagents, methods and kits for the purification of RNA, or DNA, or both, from a sample....

20060105348 - Compositions and methods for the detection and discrimination of nucleic acids: The present invention provides novel primers and methods for the detection of specific nucleic acid sequences. The primers and methods of the invention are useful in a wide variety of molecular biology applications and are particularly useful in allele specific PCR....

20060105362 - Compositions and systems for identifying and comparing expressed genes (mrnas) in eukaryotic organisms: The invention comprises compositions and systems to identify and compare expressed genes in a given in vivo or in vitro RNA sample, as well as the relative difference in mRNA expression between two or more samples, where desired. Furthermore, the invention comprises compositions and systems to identify novel genes. The...

20060105342 - Computerized systems for formation and update of databases: An apparatus for creating and maintaining a database containing information relating to residential properties includes an AVM device configured to receive information relating to residential properties, producing AVM values for each property and updating an AVM database with the AVM values....

20060105384 - Detection of dna damage: The health condition of a living organism is detected by electrochemically analyzing samples from selected areas of the body of said living organism for elevated free levels of nucleotide excision products resulting from DNA or RNA damage....

20060105349 - Device and method for purification of biological materials: An apparatus, method and kit for isolating a biomolecule from a sample. The sample comprises a complex biological material, which includes insoluble matter. Some embodiments of the apparatus and kit include a reservoir and means for capturing the biomolecule either contained within or coupled to the reservoir. The reservoir can...

20060105360 - Diagnosis and treatment of cancers with microrna located in or near cancer associated chromosomal features: MicroRNA genes are highly associated with chromosomal features involved in the etiology of different cancers. The perturbations in the genomic structure or chromosomal architecture of a cell caused by these cancer-associated chromosomal features can affect the expression of the miR gene(s) located in close proximity to that chromosomal feature. Evaluation...

20060105330 - Diagnostics and remedies for malignant brain tumor: It is intended to provide a diagnosis method and diagnostics for cancers such as human glioma and a therapeutic method and remedies for cancers such as human glioma by identifying tumor suppressive genes or cancer genes useful in diagnosing or treating cancers such as human glioma. Tumor suppressive genes such...

20060105361 - Donor yeast strain for transfer of genetic material: The invention provides a universal yeast donor strain that contains a conditional centromere and a URA3 allele on every chromosome. This strain was constructed in four rounds of crosses of individual conditional chromosome strains using a novel tetrad-based screen to identify segregants in which all marked chromosomes were contained in...

20060105355 - Electrode array device having an adsorbed porous reaction layer having a linker moiety: There is disclosed an electrode array device having an adsorbed porous reaction layer having a linker attacher thereto for improved synthesis quality. The array comprises a plurality of electrodes on a substrate, wherein the electrodes are electronically connected to a computer control system. The array has an adsorbed porous reaction...

20060105329 - Gene panel participating in liver regeneration: A gene panel comprising a group of genes of which expression levels change in hepatocytes during liver regeneration as compared with those in a normal state is produced by the following steps: (a) the step of measuring expression levels of various genes in hepatocytes of a model animal in a...

20060105346 - Human prostaglandin fp receptor variants and methods of using same: The invention provides an isolated polypeptide containing the amino acid sequence of SEQ ID NO: 17, 18, 19, 20, 21, 22, 23, 24, or 25. The invention also provides an isolated polypeptide containing an amino acid sequence having at least 50% amino acid identity with SEQ ID NO: 14 and...

20060105336 - Isolation and identification of t cells: The present invention relates to improved autologous T cell vaccines and improved methods for their production. The invention is also directed to methods for treating autoimmune diseases such as multiple sclerosis or rheumatoid arthritis using autologous T cell vaccines. The invention is further directed to the diagnosis of T cell...

20060105328 - Mab-based dot-elisa method and assay kit for the detection of viruses: A monoclonal antibody-based Dot-ELISA assay for the rapid detection of animal viruses such as avian influenza virus. The assay includes applying a specimen suspected of containing an animal virus on a porous membrane and treating the specimen with a solution of citric acid or lactic acid and a solution containing...

20060105359 - Method and apparatus for automated pre-treatment and processing of biological samples: The present invention concerns a method and apparatus for automatic processing a biological sample on a carrier, perhaps robotically, by applying predetermined amounts of reagents in a predetermined sequence according to a processing protocol, the processing including pre-treatment steps, under the control of an adaptive processing control system using a...

20060105338 - Method and apparatus for correcting position and attitude of object to be held: There is provided a method of correcting position and attitude of an object to be held capable of reducing the number of image processing steps and simplifying the method of correcting shifts in the position and attitude of the object with respect to a holding member. The object to be...

20060105371 - Method and device for protein delivery into cells: Methods for performing surface-mediated protein delivery into living cells, and fabricating protein-transfected cell cluster arrays are provided. The method comprises providing a protein-containing mixture; depositing said protein-containing mixture onto a surface at defined locations; affixing the protein-containing mixture to the surface as microspots; and plating cells onto the surface in...

20060105368 - Method and device for simultaneously molecularly cloning and polylocus profiling of genomes or genome mixtures: A method for amplifying genetic material to characterize complex populations of nucleic acids and a method for applying these characterizations to applications. The characteristics of a population of nucleic acids may be an index of disease states as for example when pathogens cause nucleic acid release into body fluids or...

20060105367 - Method and markers for the diagnosis of graft versus host disease (gvhd): A method for the diagnosis of GvHD, the method comprising: c) measuring the presence or the absence of a polypeptide marker in a urine sample, wherein the polypeptide marker is selected from the group of polypeptide markers shown in table 1, and d) comparing the probability of the presence of...

20060105351 - Method and system for nucleic acid detection: The present invention relates to a method of detecting nucleic acids comprising suspending at least one recognizable target nucleic acid in a suspending solution containing polymeric particles marked with a probe, wherein the probe has an affinity for the target nucleic acid, adding a recognition unit-peroxidase conjugate marker to the...

20060105350 - Method and system for sorting and separating particles: The present invention relates to a method of particle separation comprising preparing a suspension of particles containing at least one recognizable target particle in a suspending solution, labeling the target particle with a conjugate marker, wherein the conjugate marker comprises at least one recognition unit for the recognizable target particle...

20060105345 - Method for diagnosing lung transplantation rejection: The invention relates to a method for diagnosing lung transplantation rejection comprising determining the amount of Hepatocyte Growth Factor (HGF) in a body fluid or tissue sample of a patient who has undergone lung transplantation....

20060105333 - Method for diagnosis of intestinal-type gastric tumors: Objective methods for detecting and diagnosing intestinal-type gastric cancers are described herein. Also described is method for predicting the presence or absence of lymph node metastasis (i.e., identifying the metastatic phenotype). In one embodiment, the diagnostic method involves the scoring of gene expression profiles that discriminate between lymph node positive...

20060105352 - Method for sensitive detection of multiple biological analytes: The present invention relates to a method of detecting biological analytes comprising suspending a target analyte in a suspending solution containing polymeric particles marked with a probe, wherein the probe has an affinity for said target analyte; adding recognition unit-peroxidase conjugate marker to the suspending solution; forming a complex of...

20060105347 - Method for the production of fusion proteins in transgenic mammal milk: Desirable fusion proteins can be produced in and purified from the milk of transgenic animals. The peptides are made as fusion proteins with a suitable fusion partner such as human alpha-fetoprotein. The fusion partner protein acts to promote and increase the half-life of the overall molecule as well as having...

20060105334 - Method of determining the copy number of a nucleotide sequence: The invention relates to a method of determining of accurately determining the copy number of a nucleotide sequence I in a sample using an amplification technique, such as PCR. In addition, a second nucleotide sequence II is also measured and calibration curves for each are made, from which the relative...

20060105370 - Method of nucleic acid typing for selecting registered donors for cross-matching to transfusion recipients: Disclosed are a method and an algorithm for genetic cross-matching based on the comparison of recipient and donor genotypes—and the underlying combinations of alleles and haplotypes. The method of the invention, rather than focusing on phenotype prediction, instead relies on a comparison of genetic variants identified in the recipient and...

20060105369 - Methods and kits for identifying enterohaemorrhagic escherichia coli o157:h7 strain: Disclosed is a method for identifying the presence of Enterohaemorrhagic E. coli O157:H7 strain using primers or a probe prepared in accordance with SEQ ID NO: 1 or a sequence homologous thereto. A kit for identifying Enterohaemorrhagic E. coli O157:H7 strain is also disclosed....

20060105380 - Methods and systems for dynamic gene expression profiling: The invention provides compositions, methods and systems for dynamic transcription profiling of two or more samples. The method of the invention comprises the uses of sample-specific primers for cDNA synthesis and for subsequent amplification of the synthesized cDNAs. The levels of abundance of genes are compared between samples for the...

20060105363 - Methods for determining transcriptional activity: In some embodiments of the invention, methods are provided to interrogate the transcriptional activity. The methods employ hybridization of a large number of oligonucleotide probes with nucleic acid derived from RNAs in a cellular compartment....

20060105343 - Methods for diagnosis and prognosis of cancer: We have discovered a protein in humans, herein referred to as thymosin R16 (SEQ ID NO: 1), that is expressed in human prostate cancer tumors but not in specimens of benign prostate hyperplasma (BPH) tissues. In contrast, prostate specific antigen (PSA), the gold standard of prostate cancer diagnosis, is highly...

20060105331 - Methods for improving rna transcription reactions: Methods are described for eliminating single-stranded oligonucleotides from a sample prior to RNA transcription, thereby reducing non-template derived production of RNA. In one embodiment, a sample containing the template for RNA transcription is treated with one or more exonucleases to remove single-stranded oligonucleotides from the reaction mixture prior to RNA...

20060105339 - Methods for measuring the rates of replication and death of microbial infectious agents in an infected: The present invention provides methods and kits useful for determining rates of replication and destruction of an infectious agent within an infected host organism. In the methods of the invention, an isotopically-labeled precursor molecule is administered to an infected host, and is given sufficient time to pass into the host's...

20060105356 - Methods for reducing evaporation in wet processing steps: Methods of contacting a surface of an object with a liquid are provided. Aspects of the invention include positioning an object in a reaction chamber and flowing a liquid across a surface of the object, where at least a portion of the flowing step occurs in the presence of a...

20060105382 - Microfluidic channels with attached biomolecules: An exemplary system and method for bonding substrate layers in the presence of chemically active species to form functionalized microfluidic surfaces is disclosed as comprising inter alia a first substrate (100), a second substrate (200), a chemically functional species (120) attached to first substrate (100), and a radiatively absorptive mask...

20060105344 - Modulation of deubiquitinase family members: Modulation of Deubiquitinase Family Members The present invention relates to the therapeutic treatment of conditions by modulating members of the deubiquitinase family, and also to assays for identifying substances which may be useful in such treatments, as well as to the treatment of cylindromatosis and more generally to the modulation...

20060105353 - Non-invasive prenatal fetal cell diagnostic method: The present invention provides methods for prenatal diagnosis. The method includes distinguishing fetal cells from maternal cells. The fetal cells may be analyzed to measure, for instance, the presence or absence of genetic markers....

20060105376 - Novel full-length cdna: Novel full-length cDNAs are provided. 2443 cDNA derived from human have been isolated. The full-length nucleotide sequences of the cDNA and amino acid sequences encoded by the nucleotide sequences have been determined. Because the cDNA of the present invention are full-length and contain the translation start site, they provide information...

20060105340 - Novel tumor suppressor gene and compositions and methods for making and using the same: The present invention relates to the identification and cloning of ARTS-1, a novel tumor suppressor gene, to isolated proteins encoded by ARTS-1, and to methods of making and using the same....

20060105358 - Nucleic acid cloning: The present invention provides an improved system for linking nucleic acids to one another. In particular, the present invention provides techniques for producing DNA product molecules that may be easily and directly ligated to recipient molecules. The product molecules need not be cleaved with restriction enzymes in order to undergo...

20060105381 - Polymorphisms of the octn1 cation transporters associated with inflammatory bowel disorders: The invention provides a method for diagnosing Inflammatory Bowel Diseases, using genetic markers that are implicated in severe, early-onset Crohn's Disease (CD). The invention also provides coding sequence mutations in the OCTN1 gene that significantly reduces its ability to transport the organic cation carnitine. The invention further provides mutations in...

20060105379 - Polynucleotide encoding a novel metalloprotease highly expressed in the testis, mmp-29: The present invention provides novel polynucleotides encoding MMP-29 polypeptides, fragments and homologues thereof. Also provided are vectors, host cells, antibodies, and recombinant and synthetic methods for producing said polypeptides. The invention further relates to diagnostic and therapeutic methods for applying these novel MMP-29 polypeptides to the diagnosis, treatment, and/or prevention...

20060105354 - Real-time quantification of multiple targets on a micro-array: The present invention relates to a method and apparatus for monitoring a real-time quantification of multiple target molecules during their binding on capture molecules of a micro-array. The method comprises the steps of: placing, in a chamber (14), a support (15) having fixed upon its surface a micro-array comprising at...

20060105374 - Rhob variants and methods of use: In one aspect, the present invention concerns RhoB variant polypeptides and isolated degenerate polynucleotides encoding the RhoB variant polypeptides. In another aspect, the present invention concerns nucleic acid constructs containing a polynucleotide encoding a RhoB variant polypeptide, and host cells genetically modified to express such polynucleotides. In another aspect, the...

20060105377 - Screening to optimize rnai: Methods and compositions are provided for screening RNAi molecules for efficiency of modulation, particularly inhibition, of expression of genes. The gene for the target protein is fused to a DNA sequence encoding a small fragment of β-galactosidase. The fragment is competent to complex with a large fragment of β-galactosidase to...

20060105337 - Solid phase based nucleic acid assays combining high affinity and high specificity: The invention relates to methods for detection of nucleic acids on a solid phase, combining high affinity and high specificity. More particularly, the invention relates to methods combining high-affinity hybridization with highly specific enzymatic discrimination in solid phase based nucleic acid assays. This invention further relates to kits containing the...

20060105366 - Specific base sequence detection method and primer extension reaction detection method: A specific base sequence detection method, comprising preparing a sample solution including a target nucleic acid, a primer for amplifying a specific base sequence and whose end has a site to be coupled to an electrode, and nucleotide; extending the primer if the specific base sequence is present in the...

20060105378 - Tenascin-c nucleic acid ligands: Methods are described for the identification and preparation of nucleic acid ligands to tenascin-C. Included in the invention are specific RNA ligands to tenascin-C identified by the SELEX method. Further included in the invention are methods for detecting the presence of a disease condition in a biological tissue in which...

20060105357 - Tissue sensor and uses thereof: Described are assemblies for screening a compound for bioactivity, the assemblies comprising a tissue and a sensor. A change in a biological parameter is measured by the sensor, such that a change in a parameter occurring when the tissue is contacted with a candidate compound is detected by the sensor....

20060105341 - Trap-tagging: a novel method for the identification and purification of rna-protein complexes: Conventional methods for the isolation and identification of specific RNA-protein complexes are plagued by a number of problems not encountered in genomics or proteomics. Here we describe a two step affinity purification method used to isolate RNA-protein complexes. The TRAP (Tandem RNA Affinity Purification) tag is a dual RNA tagging...

20060105332 - Treatment of tissue specimens: Apparatus for treating tissue specimens comprises a stationary outer casing (2) in the top of which is positioned an annular trough (3) for holding a liquid. Rotatably mounted in the outer casing (2) is a central hub (5) supporting a disc-like lid (6) the underside of which carries a magnet...

20060105385 - Xenobiotic related induction of gene expression: An isolated nucleic acid molecule comprising a nucleotide sequence encoding a transcriptional enhancer of cytochrome P450 (P450) CYP3A4 production or expression, and uses of the nucleic acid molecule for screening compounds for xenobiotic induction of CYP3A4 expression in cells and animals....

20060105391 - Device and method for separating molecules: An apparatus and method for separating a biomolecule from one or more contaminants in a sample. The apparatus can include a unitary device comprising a plurality of fractionation devices, each reservoir comprising a filter, each reservoir adapted to contain a solid phase, the solid phase adapted to separate the biomolecule...

20060105392 - Differential diagnosis of vitamin b12, vitamin b6, and folic acid disorders: The invention concerns a method for determining vitamin B12, B6 or/and folic acid disorders and in particular the differential diagnosis of vitamin B12, vitamin B6 or/and folic acid disorders by means of three or four independent parameters. The differential diagnosis can be used to detect a vitamin B12, vitamin B6...

20060105388 - Incorporation of hapten groups during the production of carriers for the determination of analytes: The invention relates to a method for producing a carrier, especially microfluidic carrier, for the determination of analytes....

20060105393 - Ligand-receptor tracking assays: The present invention provides an image-based method of screening for compounds based on the measurement of internalization of a fluorescently labeled ligand. It further provides a multiplex method for high content screening of compounds wherein at least two cellular parameters are measured....

20060105389 - Method for obtaining single chain antibodies to human interferon alpha2b: A bacterial high-expression system which is applicable for simultaneous screening of large numbers of recombinant clones from combinatorial antibody libraries is disclosed. The method pertains to screening of single chain antibodies from libraries expressed in the periplasm of E. coli by secretion. By this approach, approximately 104 clones can be...

20060105397 - Method for the detection of stress biomarkers including cortisol by fluorescence polarization: The inventive subject matter relates to a competitive method measuring stress biomarkers in bodily fluids including serum, urine and oral fluids including saliva. The inventive method measures biomarkers including cortisol, melatonin and secretory IgA by fluorescence polarization fluorescence lifetime analysis or fluorescence resonance energy transfer....

20060105390 - Method of characterizing heterogeneity in receptor-ligand interactions and related method of assay signal histogram transformation: Disclosed is a method for increasing the degree of confidence in receptor-ligand and probe-target interaction assays involving ligand or target capture by a heterogeneous population of receptors or probes displayed on solid phase carriers. The method comprises a process of establishing a weight function reflecting the heterogeneity in the receptor...

20060105394 - Methods and compositions for treatment and prevention of major depressive disorder: The present invention relates to methods of diagnosing, prognosing or treating diseases or disorders in which elevated levels of Abeta protein, including abeta1-42 are prevalent. In particular, the present invention relates to methods of diagnosing, prognosing or treating a major or minor depressive episode/disorder attributed to elevated levels of Abeta...

20060105395 - Methods and systems for positioning microspheres for imaging: Various methods and systems for positioning microspheres for imaging are provided. One system includes a filter medium that includes openings. The openings are spaced in a substantially equidistant manner across the filter medium. The system also includes a flow subsystem coupled to the filter medium. The flow subsystem is configured...

20060105396 - Novel epithelial tissue imaging agent: Targeting molecules are provided for use in delivering imaging agents to epithelial tissue. The targeting molecule comprises a polypeptide that forms a closed covalent loop, contains at least three peptide domains having β-sheet character, each of the domains being separated by domains lacking β-sheet character. The targeting molecule specifically binds...

20060105387 - Transferrin fusion proteins libraries: Modified fusion proteins of transferrin and therapeutic proteins or peptides with increased serum half-life or serum stability are disclosed. Preferred fusion proteins include those modified so that the transferrin moiety exhibits no or reduced glycosylation, binding to iron and/or binding to the transferrin receptor....

20060105399 - Materials and procedures for the purification of cells: The subject invention provides new materials and methods for the efficient isolation and purification of stem cells. Specifically, conductive immunopolymers with stem cell specific antibodies can be used to remove stem cells from biological fluids....

20060105398 - Methods of identifying compounds that modulate igg mediated mast cell activation: The present invention relates to methods for identifying compounds capable of modulating Fcg receptor signaling pathway in mast and/or basophil cells. The invention further relates to methods for identifying compounds for use as therapeutic agents in the treatment of disorders related to activation of Fcg receptor signaling cascade....

20060105402 - Blood type method system and device: A method for the detection and/or visualization of particle agglutination in a particle suspension is provided, comprising placing a volume of the particle suspension and a volume of a solution or suspension containing an agglutinating agent at substantially the same selected location on a surface of a filter constructed so...

20060105403 - Braca1/acc alpha molecular complexes, diagnostic and therapeutic applications: The invention concerns a molecular complex comprising: a first polypeptide including the sequence of amino acids 1640 to 1863 the human BRCA1 protein or a similar sequence of amino acids of the protein BRCA1 in another animal species, and a second polypeptide including a fragment of the ACC-α protein capable...

20060105404 - Fluorescence polarization assay for determining histidine decarboxylase activity: A fluorescence polarization assay for determining the HDC modulating activity of a candidate compound comprising the steps of: a) providing a reaction mixture comprising a HDC, histidine, a fluorescently labeled histamine probe, a candidate compound and an anti histamine antibody having selectivity for histamine at least 10 fold greater than...

20060105405 - Migration inhibitory factor in serum as a tumor marker for prostate, bladder, breast, ovarian, kidney, pancreatic and lung cancer and for diagnosis of endometriosis: Macrophage migration inhibitory factor (MIF) has been found to be overexpressed in blood of individuals having various cancers, including prostate, PIN, lung, breast, ovary, kidney, pancreatic and bladder, as well as inflammatory diseases and endometriosis. Normal levels of MIF in serum of males and females without these cancers have been...

20060105406 - Cytoplasmic antigens for detection of candida: The present invention relates to a method and a means of diagnosing Candida infection. In particular the present invention relates to a method of diagnosing Candida infection by measuring the levels of antibody to Candida cytoplasmic antigen present in a biological sample taken from a subject at risk of, or...

20060105401 - Diagnostic method for screening complement regulatory protein levels to predict spontaneous abortion: The invention provides a method for the early detection of pregnancy failure, spontaneous abortion or premature birth by determinations of complement regulatory protein levels. A kit for use in rapid identification of these pregnancy complications is also provided....

20060105400 - Elisa kits for detecting collagenase 3 as a proenzyme and in an activated form in body fluids and cell culture supernatants: The invention relates to ELISA kits for detecting pro-collagenase 3 and activated collagenase 3 in body fluids, especially in human serum and synovial fluid, and in cell culture supernatants, and monoclonal antibodies which specifically recognise said antigens. Said ELISA kit comprises the following separately packed elements: a) a solid carrier...

20060105408 - Myeloperoxidase, a risk indicator for cardiovascular disease: Diagnostic tests for characterizing an individual's risk of developing or having a cardiovascular disease. In one embodiment the present diagnostic test comprises determining the level of myeloperoxidase (MPO) activity in a bodily sample obtained from the individual or test subject. In another embodiment, the diagnostic test comprises determining the level...

20060105407 - Protein chip for analyzing interaction between protein and substrate peptide thereof: A protein chip of a S—L—SP form wherein a substrate peptide (SP) is immobilized on a solid substrate (S) by the mediation of a linker protein (L). Such chip is usefully employed in a method for analyzing the interaction between a reactive protein and its substrate peptide involving the steps...

20060105410 - Sulfonate compound and fluorescent probe using the same: In the formula (I), an atomic group A-O is an atomic group that forms a fluorescent compound upon cleavage of a covalent bond with a sulfonyl group. There may be one or plural atomic groups B—SO3— bound to an atomic group A. B is a ring substituted by one or...

20060105409 - Ubiquitin ligase assay: The invention relates to assays for measuring ubiquitin ligase activity and for identifying modulators of ubiquitin ligase enzymes....

20060105411 - Method of detecting early pregnancy at high accuracy by measuring hcg and hyperglycosylated hcg concentrations equally: The present invention relates to methods for analyzing concentrations of hyperglycosylated hCG (H-hCG) and hCG equally in blood, serum or urine samples, preferably urine samples, from female patients to determine with an unexpectedly high measure of accuracy whether or not the patient is pregnant and to appropriately monitor hCG levels...

20060105412 - Sulfonate compound and fluorescent probe using the same: one or a plurality of atomic groups B-SO3— are bonded to an atomic group A, B is a ring that is substituted by one or a plurality of electron-withdrawing groups, the electron-withdrawing group is at least one selected from the group consisting of an alkyl halide group, a nitro group...

20060105414 - Inhibition of b-cell maturation and antibody production: The present invention provides methods and compositions for the inhibition of B-cell maturation and antibody production without interfering with innate or T-cell mediated immunity....

20060105413 - Rick3 nucleic acids and proteins: The present invention related to methods and compositions for modulating cellular signaling. In particular, the present invention relates to PKK and RICK3 proteins. The present invention further relates the to use of PKK and RICK3 proteins in modulating NF-κB signaling. The present invention thus provides novel targets for drug screening...

20060105415 - Method for single oxygen atom incorporation into digested peptides using peptidases: Optimized enzymatic conditions incorporate a single oxygen atom into digested peptides using a peptidase. The incorporation of a single oxygen atom is especially useful for proteolytic 18O labeling in comparative proteomics. The optimized proteolytic 18O labeling minimizes the generation of a mixture of isotopic isoforms of the peptides resulting from...

20060105416 - Methods, compositions and kits pertaining to analyte determination: This invention pertains to methods, kits and/or compositions for the determination of analytes by mass analysis using unique labeling reagents or sets of unique labeling reagents. The labeling reagents can be isomeric or isobaric and can be used to produce mixtures suitable for multiplex analysis of the labeled analytes....

20060105420 - Compositions and methods for enhancement of transdermal analyte flux: The present invention relates to compositions for use in analyte monitoring devices. These compositions are useful to increase the flux of analyte across skin, tissue or mucosal surfaces. The compositions include hydrogels and collection reservoir systems comprising ionically conductive materials. The present invention also includes methods of making/manufacturing hydrogels or...

20060105418 - Immobilization support, process for producing the same, electrode, process for producing the same, electrode reaction utilizing apparatus and process for producing the same: An immobilization carrier containing an electron acceptor compound is used in addition to glutaraldehyde and poly-L-lysine to immobilize an enzyme and an electron acceptor compound simultaneously to an electrode. For example, here are used diaphorase as the enzyme and 2-amino-3-carboxy-1,4-naphthoquinone (ACNQ) as the electron acceptor compound....

20060105417 - Stable cytochrome p450 24 (cyp24) expressing cell line and methods and uses thereof: The invention relates to a stable recombinant host cell comprising a CYP24 nucleic acid. The host cell is preferably a mammalian cell or an insect cell. The cell is useful for identifying modulators of CYP24 that can be used in the treatment of CYP24 expression-related medical conditions....

20060105419 - Use of a glutathione peroxidase 1 as a marker in cardiovascular conditions: The present invention relates to materials and procedures for evaluating patients suffering from cardiovascular conditions, particularly acute coronary syndromes. In particular, the presence, amount, or enzymatic activity of glutathione peroxidase-1 in a patient sample, alone or in combination with one or more other markers, provides diagnostic and/or prognostic information. While...

20060105421 - Enzymatic measurement of imatinib mesylate: The present invention provides a method for the enzymatic measurement of protein tyrosine kinase inhibitors in biological samples. The present invention also provides assay reagents and packaged kits useful for performing enzymatic measurement of imatinib mesylate and other bcr-abl protein kinase inhibitors in biological samples....

20060105422 - Homocysteine assay: The present invention provides an improved method of assessing/quantifying the amount of homocysteine in a body fluid sample via an enzymatic assay which comprises reducing background signal by treatment with one of the following: a reducing agent, a pyruvate deactivating agent, heat treatment, or by lyophilising or immobilizing the homocysteine...

20060105428 - Alkaline protease: The present invention relates to genes expressing an alkaline protease having a prepro sequence, vectors containing the same, and transformants containing said vector. The present invention also relates to a method for producing an alkaline protease....

20060105425 - Aspergillus niger promoter for expressing genes in host cells: The present invention relates to isolated promoter sequences, particularly a promoter isolated from Aspergillus niger designated herein as A4-L or A4 and DNA constructs and vectors including the same....

20060105426 - Mcfp encoding nucleic acids, polypeptides, antibodies and methods of use thereof: mcFP encoding nucleic acids, polypeptides and antibodies immunologically specific therefore are disclosed. Methods of use thereof are also provided....

20060105423 - Microorganisms as carriers of nucleotide sequences coding for cell antigens used for the treatment of tumors: The invention relates to a microorganism with a nucleotide sequence coding for a cell antigen in which the following components are inserted and are expressible: I) a nucleotide sequence coding for at least one epitope of an antigen of a tumor cell and/or a nucleotide sequence for at least one...

20060105424 - Process for production of cephalosporin c: The present invention relates to isolated nucleic acid molecules which code for a new protein from Acremonium chrysogenum, to vectors which comprise such a nucleic acid molecule, to Acremonium chrysogenum host cells which have been transformed with such a vector and to a method for production of cephalosporin C using...

20060105427 - Secreted and transmembrane polypeptides and nucleic acids encoding the same: The present invention is directed to secreted and transmembrane polypeptides and to nucleic acid molecules encoding those polypeptides. Also provided herein are vectors and host cells comprising those nucleic acid sequences, chimeric polypeptide molecules comprising the polypeptides of the present invention fused to heterologous polypeptide sequences, antibodies which bind to...

20060105429 - Fusion polypeptides of human serum albumin and a therapeutically active polypeptide: Biologically active polypeptides comprising a therapeutically active polypeptide fused to human serum albumin or a variant thereof, methods for the preparation thereof, nucleotide sequences encoding such fusion polypeptides, expression cassettes comprising such nucleotide sequences, self-replicating plasmids containing such expression cassettes, and pharmaceutical compositions containing said fusion polypeptides....

20060105431 - Polymer grafting by polysaccharide synthases using artificial sugar acceptors: The present invention relates to methodology for polymer grafting by a polysaccharide synthase and, more particularly, polymer grafting using the glycosaminoglycan synthases from Pasteurella multocida. The methodology of the present invention includes providing an enzymatically active glycosaminoglycan synthase enzyme from Pasteurella multocida, providing a synthetic, artificial acceptor for the glycosaminoglycan...

20060105430 - Rationally designed heparinases derived from heparinase i and ii and methods of sequencing therewith: Modified heparinases having altered binding specificity and activity are provided. Isolated nucleic acids encoding the same as well as vectors and host cells are provided. Methods for using the modified heparinases are also provided....

20060105432 - Method for the production of vitamin b12: The present invention relates to a method for the production of vitamin B12 by means of a culture comprising a genetically modified Bacillus megaterium strain, to a genetically modified Bacillus megaterium strain, and to vectors for its preparation....

20060105433 - Rapid thermocycler: A thermocycler is provided, that in one embodiment has separate heat exchangers for each thermocycler target temperature, and a cold boost heat exchanger and a hot boost heat exchanger. Fluid conduit is used to circulate fluid through the appropriate heat exchanger and through a sample holder containing at least one...

20060105434 - R-hydroxynitrillyases havign improved substrate tolerance and the use thereof: The invention related to R-hydroxynitrillyases from the family of Rosaceae that are characterized by an improved substrate tolerance and increased stability. In the active center of the R-hydroxynitrillyases either a) an alanine group is substituted by glycine, valine, leucine, isoleucine, or phenylalanine or b) a phenylalanine group is substituted by...

20060105435 - Process for producing optically active alpha-methylcysteine derivative: The present invention provides a simple industrial process for producing an L- or D-optically active α-methylcysteine derivative or its salt, which is a useful pharmaceutical intermediate, from readily available, inexpensive raw materials. In a process for producing an L- or D-optically active α-methylcysteine derivative or its salt, a racemic N-carbamoyl-α-methylcysteine...

20060105436 - Polyketides and their synthesis and use: The rapamycin gene cluster is an example of a gene cluster which includes a gene (rapL) leading to the formation of a precursor compound (pipecolic acid, in this case) which is required for inclusion in the larger product (rapamycin) produced by the enzymes encoded by the cluster. We have produced...

20060105437 - Process for producing theanine: To provide a new method for efficiently producing theanine, by which theanine is simply and industrially advantageously produced. Pseudomonas citronellosis GEA, which is newly separated/selected from natural soil, belongs to the genus, Pseudomonas, and the species citronellosis and is theanine producing bacteria with activities of transferring γ-glutamyl group. Theanine is...

20060105438 - 1,3-specific lipase powder, methods for producing the same and use thereof: A method for producing a lipase powder, which comprises the step of spray drying an aqueous solution containing a lipase adjusted to pH 6 to 7.5; the lipase powder which is a 1,3-specific lipase derived from Rhizomucor miehei, spherical, and contains not more than 10% by weight of moisture; and...

20060105439 - Bioconversion of xylan and levulinic acid to biodegradable thermoplastics: Biodegradable polyesters are produced by microbial fermentation using xylose and levulinic acid as carbon sources....

20060105440 - Method of producing polyhydroxyalkanoate: The present invention has its object to provide a method of producing PHA by extracting, separating, and purifying PHA from biomass containing PHA having an weight average molecular weight of more than 2,000,000, by which smooth stirring can be carried out during extraction and filterability of a residue becomes good...

20060105441 - Process for the preparation of indole derivatives by enzymatic acylation: A process for the preparation of compounds of formula (1), by enzymatic acylation to form compounds of formulae (3a) and (3a) and then reacting the compound of formula (3a) with a compound introducing the radical of formula —CH2—COOR8, R8 being an organic radical, and then reducing, and optionally hydrolysing, the...

20060105442 - Promoters and proteins from clostridium thermocellum and uses thereof: The present invention relates to an inducible and a high expression nucleic acid promoter isolated from Clostridium thermocellum. These promoters are useful for directing expression of a protein or polypeptide encoded by a nucleic acid molecule operably associated with the nucleic acid promoters. The present invention also relates to nucleic...

20060105443 - Process for obtaining biosynthesized lycopene from bacterial cells and the purified lycopene of the same: This invention is related to a process for isolating and purifying lycopene, and more particularly to a process for large-scaled isolation and purification of the biosynthesized lycopene from bacterial cells via simple steps with high purity and recovery. The steps in the process of the present invention comprises: isolating a...

20060105444 - O-fucosyltransferase: The present invention describes the identification, purification, recombinant production and characterization of novel O-fucosyltransferase enzymes....

20060105445 - Medium and method for enriching, purifying or depleting atp binding proteins from a pool of proteins: The present invention relates to a medium and a method for enriching ATP binding proteins, e.g. proteinkinases, from a pool of proteins, like a proteome. The medium of the present invention comprises specific inhibitors, e.g. at least one of the compounds 4-[4-(4-fluoro-phenyl)-5-pyridine-4-yl-1H-imidazole-2-yl]-benzylamine, 2-[4-(2-Amino-ethoxy)-phenylamino]-6-(2,6-dichloro-phenyl)-8-methyl-8H-pyrido[2,3-d]pyrimidine-7-one, 2-[1-(3-aminopropyl)-1H-indole-3-yl)maleinmide, 3-[1-(3-Aminopropyl)-1H-indol-3-yl]-3-(1H-indol 3-yl)-maleinmide, 3-[1-(3-Aminopropyl)-1H-indol-3-yl]4-(1-methyl-1H-indol-3-yl) maleinimide, 3-(8-Aminomethyl-6,7,8,9-tetrahydropyrido-[1,2-a]-indol-10-yl) (1-methyl-1H-indol...

20060105446 - Bacillus subtilis var. chungkookjang producing high molecular weight poly-gamma-glutamic acid: Bacillus subtilis var. chungkookjang, accession no. KCTC 0697BP, a salt-resistant B. subtilis variant isolated from “chungkookjang,” a traditional Korean fermented soy bean paste. This B. subtilis variant produces poly-gamma-glutamic acid (or poly-gamma-glutamate, γ-PGA), an edible, soluble, anionic, and biodegradable high molecular-weight polymer. Bacillus subtilis var. chungkookjang produces γ-PGA with a...

20060105447 - \"transformer\" aerobic digestion method, a system for treating biodegradable waste material through aerobic degradation: This method facilitates the cost effective treatment of liquid and solid waste in a landfill or composting facility. The ability to distribute the oxygen to the waste and remove the spent waste is the key to the process. The process can digest a megafill or treat massive green waste pile....

20060105448 - Reactor device: The invention relates to a reactor device (1). In order to keep the equipment costs low and to make it possible to handle a plurality of cultivations during a short time period, the device (1) comprises a channel and means (17) for delivering fluid to monitoring means (4, 5); reactor...

20060105449 - Biochip having an electode array on a substrate: A biochip for electrical, e.g., capacitive, detection of biochemical molecules has an electrode array on a substrate, the electrodes of the electrode array being each connected through the substrate to an electrically conductive contact surface via an electrical conductor. The contact surfaces are situated on the side of the substrate...

20060105450 - Vessel loading/unloading: Apparatus is described for loading or unloading a vessel, say a flask, on to or from a selected position on a support arranged to oscillate substantially in a plane. The apparatus includes a transporter in the form of a robotic arm having a gripper unit for gripping the flask. A...

20060105451 - Genetically engineered p30 antigen improved antigen cocktail, and uses thereof: The present invention relates to a genetically engineered P30 antigen and a combination or mixture of antigens (e.g., the genetically engineered P30 antigen and P35) that may be used in the detection of IgM and/or IgG antibodies to Toxoplasma gondii. Furthermore, the present invention also relates to methods of using...

20060105453 - Coating process for microfluidic sample arrays: A differentially coated device for conducting a plurality of nano-volume specified reactions, the device comprising a platen having at least one exterior surface modified to a specified physicochemical property, a plurality of nano-volume channels, each nano-volume channel having at least one interior surface in communication with the at least one...

20060105452 - Convective flow tissue assembly: The present invention provides for an improved in vitro tissue assembly system and related methods that includes and uses a bioreactor, a porous mandrel disposed in the bioreactor, and components that provide for the circulation of culture media and cell suspensions within the bioreactor and through the porous mandrel. The...

20060105455 - Covalently attached collagen vi for cell attachment and proliferation: Surfaces useful for cell culture comprise a support to which is bound a CAR material, and, bound to the CAR material, collagen VI or a biologically active fragment or variant thereof and, optionally, one or more other ECM proteins (or fragments or variants thereof) such as elastin, fibronectin, vitronectin, tenascin,...

20060105456 - Method for purifying adenoviruses: Methods for purifying adenoviruses from a contaminated sample using a hydroxyapatite medium are provided. Sodium chloride concentrations present in buffers used throughout the method are at least 150 mM to prevent the adenovirus from irreversibly binding to the hydroxyapatite. Levels of contaminants and empty adenovirus capsids from samples purified by...

20060105454 - Method of isolating epithelial cells, method of preconditioning cells, and methods of preparing bioartificial skin and dermis with the epithelial cells or the preconditioned cells: A method of isolating epithelial cells from a human skin tissue or internal organ tissue using trypsin and ethylenediamine tetraacetic acid (EDTA) simultaneously with the application of magnetic stirring, a method of preconditioning isolated biological cells by the application of physical stimulus, i.e., strain, are provided. Epithelial cells can be...

20060105457 - Method for producing a functional neuron: The present invention relates to the production of functional neurons from adult human mesenchymal stem cells using a retinoid. A retinoid, when used in the absence of a growth factor, transdifferentiates mesenchymal stem cells into functional neurons that exhibit synaptic transmission. Moreover, polarization of the functional neurons can be achieved...

20060105458 - Caged tetracycline (derivatives), their generation, and their use for photoactivated gene expression: Transcriptionally inactivated (caged) tetracycline or tetracycline derivative, wherein the inactivation is caused by reaction of the tetracycline (derivative) with a photosensitive protection compound, said caged tetracycline or tetracycline derivative being capable to be activated again by photolysis....

20060105459 - Transgenic mice containing nttp1 phosphatase gene disruptions: The present invention relates to transgenic animals, as well as compositions and methods relating to the characterization of gene function. Specifically, the present invention provides transgenic mice comprising mutations in a NTTP1 gene. Such transgenic mice are useful as models for disease and for identifying agents that modulate gene expression...

20060099567 - Integration of sample storage and sample management for life science: Compositions and methods are disclosed for automated storing, tracking, retrieving and analyzing biological samples, including dry storage at ambient temperatures of nucleic acids, proteins (including enzymes), and cells using a dissolvable dry storage matrix that permits recovery of biologically active materials. RFID-tagged biological sample storage devices featuring dissolvable or dissociable...

20060099570 - Device and method for non-invasive measurement of the individual metabolic rate of a substantially spherical metabolizing particle: The present invention relates to methods and devices for non-invasive and non-disturbing measurements of metabolizing rates of substantially spherical metabolizing particles, such as an embryo, and to a method and device for controlling oxygen partial pressure at the level of the embryo. Furthermore, the invention relates to a method for...

20060099569 - Method and device for diagnosing oncological diseases: The invention relates to medicine and can be used for diagnosing oncological diseases, manly on early stages thereof and for evaluating a treatment efficiency. The inventive method consists in studying an aqueous solution of native plasma or a native blood serum of a patient with the aid of a laser...

20060099568 - Signals and molecular species involved in senescence: The present invention relates to (a) a method for detecting a senescent cell, which comprises measuring a relative alteration to young cell in a signal or molecular species involved in signal transduction; (b) a method; and (c) a composition for modulating cellular senescence comprising treating a senescent cell with the...

20060099571 - Stabilized bioactive peptides and methods of identification, synthesis, and use: An intracellular selection system allows screening for peptide bioactivity and stability. Randomized recombinant peptides are screened for bioactivity in a tightly regulated expression system, preferably derived from the wild-type lac operon. Bioactive peptides thus identified are inherently protease- and peptidase-resistant. Also provided are bioactive peptides stabilized by a stabilizing group...

20060099572 - Crystallization reagent matrices and related methods and kits: This invention provides methods, kits and automated systems for identifying a reagent in which a compound crystallizes, and methods for crystallizing a compound....

20060099574 - Detection and distinguishing infections bursal disease virus (ibdv) strains by molecular biology method: The present invention relates to a novel method to detect and differentiate different strains of infectious bursal disease virus (IBDV) in a chicken and other bird sample. RNA was obtained from said samples by using a pair of primer (Primer FVVC & RVVC) in a reverse transcriptase-polymerase chain reaction. Two...

20060099573 - Diagnostic assays: Diagnostic assays using IgE for detecting HIV in a baby patient or a child patient that might be infected with HIV; distinguishing between classic dengue and dengue hemorrhagic fever in a patient that is believed to have either the classic or hemorrhagic version of dengue; and detecting cognitive impairment in...

20060099614 - analysis of dna: The invention provides improved techniques for investigating DNA samples, which offers improved sensitivity and specifity. The invention provides a method of investigating single nucleotide polymorphisms in a sample of DNA, the method comprising contacting the DNA containing sample with at least one first set of primers, amplifying the DNA using...

20060099622 - 12 human secreted proteins: The present invention relates to 12 novel human secreted proteins and isolated nucleic acids containing the coding regions of the genes encoding such proteins. Also provided are vectors, host cells, antibodies, and recombinant methods for producing human secreted proteins. The invention further relates to diagnostic and therapeutic methods useful for...

20060099575 - 67 human secreted proteins: The present invention relates to novel human secreted proteins and isolated nucleic acids containing the coding regions of the genes encoding such proteins. Also provided are vectors, host cells, antibodies, and recombinant methods for producing human secreted proteins. The invention further relates to diagnostic and therapeutic methods useful for diagnosing...

20060099584 - A method for increasing the affinity of an oligonucleotide for a target nucleic acid: The present invention relates to the optimization of primer libraries. Shorter primers are annealed to template sequences and extended in order to provide primers having improved specificity. The primers of the invention have utility in DNA amplification and sequencing methods....

20060099609 - Activation of natural killer (nk) cells and methods of use: The present invention relates to filovirus VLPs and their use in activating innate immunity, specifically natural killer cells, and in enhancing an immune response to an antigen in an animal....

20060099587 - Alphavirus vectors having attentuated virion structural proteins: The present invention provides immunogenic compositions and methods that may be used to administer safer (i.e., attenuated) alphavirus vectors (such as alphavirus vectors comprising a VEE virion shell) that retain improved immunogenicity as compared with other attenuated alphaviruses (e.g., the VEE 3014 mutant, described below). In particular embodiments of the...

20060099635 - Anti-gl50 antibodies: The invention provides isolated nucleic acids molecules, designated GL50 nucleic acid molecules, which encode novel GL50 polypeptides. The invention also provides antisense nucleic acid molecules, recombinant expression vectors containing GL50 nucleic acid molecules, host cells into which the expression vectors have been introduced, and nonhuman transgenic animals in which a...

20060099606 - Assay to detect viral uncoating: A cell-free viral uncoating assay is provided....

20060099636 - Biomolecular analysis by rolling circle amplification and sers detection: The present methods, compositions and systems are concerned with biomolecule 130 detection, identification and/or quantification by rolling circle amplification (RCA) and Raman detection. In particular embodiments of the invention, the RCA is exponential RCA or linear RCA. In some embodiments of the invention, the Raman detection is SERS or SERRS....

20060099583 - Compositions and methods for identifying antiviral agents: Disclosed are compositions and methods that can be used to identify antiviral compounds. The methods can be carried out by exposing a cell that expresses a host factor to a candidate compound. If the expression or activity of the host factor, which is a protein we identified by virtue of...

20060099619 - Detection and quantification of mirna on microarrays: The present invention relates to a new method for the detection, identification and/or quantification of multiple gene-specific mRNA or stRNA, respectively, the inducers of RNAi. In particular the present invention relates to a method for detecting the presence or change in concentration of mRNA in a cell, which change may...

20060099621 - Detection of nucleic acids to assess risk for creutzfeldt-jakob disease: The present invention provides a method of detecting abnormal circulating nucleic acid profiles to assess the risk of Creutzfeldt-Jakob Disease....

20060099618 - Detection, identification and differentiation of serratia species using the spacer region: e

20060099605 - Devices and methods for isolating rna: Devices and methods for isolating nucleic acid are disclosed herein. In particular, the isolation of total cellular RNA is discussed. Additionally, devices and methods for reducing genomic DNA in a biological sample without introducing harmful contaminants, and without significantly increasing the time required for the overall procedure being performed on...

20060099591 - Diagnosis of chronic rejection: Methods are disclosed for, early diagnosing chronic rejection (CR) in a transplanted subject, monitoring CR in a transplanted subject at risk of developing CR, preventing, inhibiting, reducing or treating CR in a transplanted subject, or identifying agents for use in the prevention, inhibition, reduction or treatment of CR, based on...

20060099627 - Diagnosis of parasites: Detection of Plasmodium ssp. by analysis of extrachromosomal DNA. It has been found that the molecular composition, physical arrangements, and nucleotide sequences of the extrachromosomal plastid-like element and mitochondrial element are highly conserved in different Plasmodium ssp. The high degree of homology has been used to design genera-specific or species...

20060099585 - Diagnostic and therapeutic use of cyp11a1 for neurodegenerative diseases: The present invention discloses the differential expression of the CYP11A1 gene in specific brain regions of Alzheimer's disease patients. Based on this finding, this invention provides a method for diagnosing or prognosticating a neurodegenerative disease, in particular Alzheimer's disease in a subject, or for determining whether a subject is at...

20060099628 - Diagnostic assay for rickettsia prowazekii disease by detection of responsive gene expression: The inventive subject matter relates to a method for the detection and diagnosis of Rickettsia prowazekii infection by measuring the increased or decreased expression of specific human genes following infection by microarray or polymerase chain reaction analysis. Gene modulation profiles can be further analyzed by computer. The method permits the...

20060099626 - Dna-templated combinatorial library device and method for use: The present invention provides a device and method for synthesizing nucleic acid-templated combinatorial chemical libraries. The device includes a splitting filter having an array of immobilized capture nucleic acids and a chemical coupling filter having an array of non-specific binding features, wherein the plates are positioned to provide for alignment...

20060099586 - Eef1a2 for use in the prognosis, diagnosis, and treatment of cancer: Methods and kits for diagnosing and prognosticating cancer via detection of EEF1A2 and/or EEF1A2 are provided. Also provided are methods for treating cancer via inhibition of expression and/or activity of EEF1A2 and screening assays to identify new anticancer agents based upon their ability to inhibit EEF1A2 expression and/or activity. The...

20060099631 - Electrodes linked via oligomers to nucleic acids: The invention relates to nucleic acids covalently coupled to electrodes via conductive oligomers. More particularly, the invention is directed to the site-selective modification of nucleic acids with electron transfer moieties and electrodes to produce a new class of biomaterials, and to methods of making and using them....

20060099604 - Fluorescent affinity tag to enhance phosphoprotein detection and characterization: The invention relates to target-associative tags incorporating cysteamine as the target-associative moiety. The invention further relates to a method for producing a target-associative tag by addition of cysteamine as the target-associative moiety to another molecule or entity having a property or properties useful in discriminating or selecting between members of...

20060099589 - Gene shuffling by template switching: The present invention provides a new approach to creating novel polynucleotide sequences by point mutation and recombination in vitro of a set of parental sequences. The new polynucleotide sequences can be useful in themselves or they can be used for the templated synthesis of polymers, e.g. polypeptides composed of α-amino...

20060099595 - Highly permissive cell lines for hepatitis c virus replication: This invention relates generally to cells and cell lines that are permissive for hepatitis C virus (HCV) replication, and methods and materials for making and using them....

20060099633 - Human fcgammariib gene polymorphisms for assessing development of systemic lupus erythematosus and compositions for use thereof: The present invention provides methods for predicting the likelihood of development of systemic lupus erythematosus (SLE) in an individual, which comprise determining the sequence at one or more polymorphic positions within the human genes encoding FcγRIIB. The invention also provides isolated nucleic acids encoding FcγRIIB polymorphisms, nucleic acid probes that...

20060099579 - In situ induced antigen technology (isiat) for identification of polynucleotides expressed during infection or colonization: The invention provides compositions and methods for identifying polynucleotides and polypeptides expressed by a microbe during infection or colonization. The invention also provides compositions and methods for identifying polynucleotides and polypeptides expressed by a host organism in response to a disease state. The invention also provides methods and compositions for...

20060099611 - In vivo affinity maturation scheme: The present invention relates to the field of evolution of nucleic acids in vivo and provides methods and compositions for introducing diversity into gene products. The present invention allows generation of new sequences that have desirable properties by virtue of high frequency mutation events within a cell. The high frequency...

20060099610 - Method and kit for detecting a risk of acute myocardial infarction: Genes, SNP markers and haplotypes of susceptibility or predisposition to CHD such as AMI are disclosed. Methods for diagnosis, prediction of clinical course and efficacy of treatments for AMI using polymorphisms in the AMI risk genes are also disclosed. The genes, gene products and agents of the invention are also...

20060099581 - Method for analysis of methylated nucleic acids: A method is disclosed providing analysis of the degree of methylation within nucleic acid samples, including the degree of methylation within CpG islands. After bisulfite treatment of a nucleic acid sample to convert cytosines to uracils, multiple species of paired oliogonucleotide primers and optionally a methylation insensitive reference primer pair...

20060099612 - Method for analyzing genes of industrial yeasts: m

20060099576 - Method for diagnosing a pre-neoplastic or neoplastic lesion in transitional epithelial cells: The present invention provides a method for determining whether a subject has a pre-neoplastic or neoplastic lesion in transitional epithelial cells. The present invention further provides a method for assessing the efficacy of therapy to treat a pre-neoplastic or neoplastic lesion in transitional epithelial cells in a subject who has...

20060099577 - Method for preparative production of long nucleic acids by pcr: The invention relates to a method for preparative production of long nucleic acids by PCR. The method involves the following hybridization steps: a) a nucleic acid base sequence is hybridized on the 3′ and 5′ ends with an adapter primer; b) the product from step a) is hybridized on the...

20060099592 - Method for the synthesis of a bifunctional complex: Disclosed is a method for obtaining a bifunctional complex comprising a display molecule part and a coding part, wherein a nascent bifunctional complex comprising a chemical reaction site and a priming site for enzymatic addition of a tag is reacted at the chemical reaction site with one or more reactants,...

20060099615 - Method of detecting target nucleotide sequence, structure and preparation of the detection target used for working the detection method, and assay kit for detecting the target nucleotide sequence: The present invention provides a method of preparing a detection target structure useful for detecting a target nucleotide sequence. For example, provided is a method of preparing a detection target structure from a target nucleic acid (1). The target nucleic acid includes a target nucleotide sequence 2 in a part....

20060099593 - Method of diagnosing integration dysfunction syndrome using blood: An object of the present invention is. to provide an objective method for diagnosis of schizophrenia using gene expression in mononuclear cells of peripheral blood as an index, and this invention provide a method for diagnosing whether a test subject suffers from schizophrenia or not. The method according to this...

20060099590 - Method of diagnosing risk of restenosis after coronary angioplasty: It is intended to provide a means of diagnosing genetic risk of restenosis after coronary angioplasty which shows a high accuracy and a high estimation ratio. The risk of restenosis after coronary angioplasty is diagnosed by a method comprising the following steps: (i) the step of analyzing 2 or more...

20060099603 - Method of diagnosis/prognosis of human chronic lymphocytic leukemia comprising the profiling of lpl/adam genes: The present invention provides methods of diagnosis and prognosis of human chronic lymphocytic leukemia (CLL) in a subject a patient in need thereof. The methods of the present invention involve measuring the expression profile of two known genes: LPL and ADAM29; and comparing the ratio of their expression to diagnose...

20060099597 - Method of isolating nucleic acid having desired functional property and kit therefor: The present invention provides a method for isolating a nucleic acid having an intended functional property conveniently and rapidly, as well as a kit for carrying out the method. Specifically, the present invention provides a method comprising the steps of: (A) transferring a nucleic acid into a plurality of first...

20060099594 - Method of predicting genetic risk for hypertension: It is intended to provide a means of predicting genetic risk for hypertension at a high accuracy and high prediction possibility. Namely, risk for hypertension is predicted by a method involving the following steps: (i) the step of analyzing two or more polymorphisms selected from among 4 gene polymorphisms having...

20060099588 - Method of producing an aspartic protease in a recombinant host organism: A polypeptide comprising an aspartic protease such as chymosin which is modified so as to comprise at least one —N—X-T-glycosylation site and a method for producing said polypeptide....

20060099617 - Method of test for animal-derived ingredients in foods: The present invention relates to a method for testing the presence or absence of an animal-derived ingredient(s) in food, which comprises the step of (i) detecting whether a DNA fragment highly reserved among animals is present, and optionally the step of (ii) detecting whether a DNA fragment highly reserved among...

20060099607 - Method, apparatus and kit for analyzing sample nucleic acid: The present invention relates to a technique for detecting the presence or absence of any mutation in a sample nucleic acid having plural possible mutation sites, which is necessary for identification of a cancerous cell....

20060099598 - Methods for diagnosis and monitoring of neurological disease by detection of an encephalotoxin: Encephalotoxin produced by activated mononuclear phagocytes is present in individuals having neurological disease including neurodegenerative and neuro-inflammatory diseases, such as Azlheimer's disease (AD), HIV-1-associated dementia (HAD), Creutzfeld-Jakob disease, Mild Cognitive Impairment, prion disease, mild congnitive/motor dysfunction, acute stroke, acute trauma, or neuro-AIDS. Biochemical detection of encephalotoxin according to the methods...

20060099632 - Methods for preparation of a nucleic acid for analysis: The present invention provides a method of preparing a nucleic acid sample comprising template nucleic acid and synthetic nucleic acid for analysis wherein prior to analysis the nucleic acid sample is treated with a substance which selectively cleaves the template nucleic acid without substantially cleaving the synthetic nucleic acid. The...

20060099608 - Methods of diagnosing cardiovascular disease: The invention relates to predicting which individuals are at risk of developing atherosclerotic vascular disease, and once having disease, which individuals are at risk of experiencing plaque rupture which, depending on the site of the plaque, could produce myocardial infarction, stroke, critical limb ischemia, or other vascular event. The invention...

20060099580 - Methyl-cpg binding domain protein 2 homologs: Two novel proteins, MBD2-CTH1 and MBD2-CTH2, are described that share extensive sequence homology to MBD2. These novel proteins lack MBDs and do not bind methylated DNA, but do, however, localize to the nucleus and assemble into transcriptional repressor complexes. These properties of MBD2-CTH1 and MBD2-CTH2 demonstrate that one or both...

20060099578 - Mitochondrial biology expression arrays: This invention provides a library of genes involved in mitochondrial biology, arrays containing probes for genes involved in mitochondrial biology, methods for making such arrays, and methods of using such arrays. Genes and probe sequences involved in mitochondrial biology in humans and mice are provided. The arrays of this invention...

20060099638 - Modified carbocyanine dyes and their conjugates: Chemically reactive carbocyanine dyes incorporating an indolium ring moiety that is substituted at the 3-position by a reactive group or by a conjugated substance, and their uses, are described. Conjugation through this position results in spectral properties that are uniformly superior to those of conjugates of spectrally similar dyes wherein...

20060099616 - Modulation of exon recognition in pre-mrna by interfering with the secondary rna structure: The invention provides a method for generating an oligonucleotide with which an exon may be skipped in a pre-mRNA and thus excluded from a produced mRNA thereof. Further provided are methods for altering the secondary structure of an mRNA to interfere with splicing processes and uses of the oligonucleotides and...

20060099625 - Mt-sp1 serine protease: This invention provides a novel membrane-type serine protease (designated MT-SP1) elevated expression of which is associated with cancer. In one embodiment, this invention provides a method obtaining a prognosis or of detecting or staging a cancer in an organism. The method involves providing a biological sample from the organism and...

20060099596 - Multiplex assay detection of pathogenic organisms: The present invention is directed to a method for identification of a pathogenic organism from a predetermined group of pathogens, comprising (i) isolating a clinical sample containing at least partially purified nucleic acid, (ii) subjecting at least a first aliquot of said clinical specimen to at least one amplification and...

20060099620 - Multiplex pcr for simultaneous quantitation of human nuclear, mitochondrial, and male y-chromosome dna: A comprehensive set of human specific, target specific, multiplex PCR assays for DNA quantitation is provided. Our duplex qPCR for nDNA/mtDNA had a linear quantitation range of 100 ng to 1 pg, and our triplex qPCR assay for nDNA/mtDNA/male Y DNA had a linear range of 100 ng to 0.1...

20060099630 - Nes-1 polypeptides, dna, and related molecules and methods: Disclosed is substantially pure NES1 polypeptide and purified DNA, vectors, and cells encoding that polypeptide. Also disclosed are methods for carcinoma detection and treatment using the NES1 sequence....

20060099600 - Novel acetylcholine transporter: This invention provides novel acetylcholine transporters. The transporters are effective and useful targets to screen for modulators of cholinergic synaptic activity. Also provided are methods of modulating the activity of cholinergic synapses using modulator of acetylcholine transporter expression and/or activity....

20060099601 - Novel compositions and processes for analyte detection, quantification and amplification: This invention provides novel compositions and processes for analyte detection, quantification and amplification. Nucleic acid arrays and libraries of analytes are usefully incorporated into such compositions and processes. Universal detection elements, signaling entities and the like are employed to detect and if necessary or desirable, to quantify analytes. Amplification of...

20060099602 - Nucleic acid library and protein library: In order to achieve an object of providing a nucleic acid library and a protein library capable of supplying plural species of proteins correlated with the nucleic acid coding therefor at once, and analyzing immediately after the supply the plural species of protein in parallel, the present invention provides a...

20060099637 - Oligonucleotide and method for detecting verotoxin: An oligonucleotide capable of binding to the intramolecular structure-free region of Verotoxin type 1 RNA or Verotoxin type 2 RNA at relatively low and constant temperature, and which can be used in a constant temperature nucleic acid amplification method, is provided. Also, a simple, speedy and highly sensitive method for...

20060099582 - Peripheral-type benzodiazepine receptor expression level as an index of organ damage and regeneration: The present invention relates to methods, reagents, and kits for assessing organ damage, such as damage due to ischemia reperfusion injury, in the course of a transplantation therapy and/or for assessing organ regeneration following transplantation therapy. The invention provides a method for determining an index of organ health in the...

20060099623 - Polynucleotides and polypeptides isolated from lactobacillus and methods for their use: Novel polynucleotides isolated from Lactobacillus rhamnosus, as well as probes and primers, genetic constructs comprising the polynucleotides, biological materials, including plants, microorganisms and multicellular organisms incorporating the polynucleotides, polypeptides expressed by the polynucleotides, and methods for using the polynucleotides and polypeptides are disclosed....

20060099639 - Prolactin receptor gene as a genetic marker for increased litter size in animals: Disclosed herein are genetic markers for animal litter size, methods for identifying such markers, and methods of screening animals to determine those more likely to produce larger litters and preferably selecting those animals for future breeding purposes. The markers are based upon the presence or absence of certain polymorphisms in...

20060099599 - Role of glycogen synthase kinase-3 and tetraspanins in ethanol-induced henaviors: This invention pertains to the identification of genes that mediate an organisms behavioral response to consumption of alcohol and/or other substances of abuse. The genes include a tetraspanin Tsp42Ee gene or a homologue or analogue thereof, a tetraspanin Tsp42El gene or a homologue or analogue thereof, and a Glycogen Synthase...

20060099634 - Simplified biological evaluation method of natural and artificial chemicals by using dna injury index and apparatus therefor: A biological evaluation method for rational and simply evaluating biological harmfulness or usefulness of a great number of natural and artificial chemicals, foods, etc. This method comprises adding a known amount of 2′-deoxyguanosine (dG) to a solution containing a test substance (drug, pesticide, functional food, etc.), optionally applying UV light...

20060099640 - Slo2 and slo4, novel potassium channel proteins from human brain: The invention provides isolated nucleic acid and amino acid sequences of Slo2 and Slo4, members of the Slo family of potassium channel proteins, also known as “maxi” or BK potassium channel proteins. Also provided herein are antibodies to Slo2 and Slo 4, methods of detecting Slo2 and Slo 4, methods...

20060099624 - System and method for providing personalized healthcare for alzheimer's disease: A system and method for providing personalized health care for Alzheimer's disease (AD) are provided. A method for providing personalized healthcare to a patient suspect of having or having AD, includes: receiving heterogeneous data of the patient; fusing the heterogeneous data by using one of an information fusion or machine...

20060099629 - Uropathogenic e. coli d-serine detoxification operon: Disclosed are methods of detecting uropathogenic E. coli genes that are differentially expressed in response to D-serine. Also disclosed are methods of characterizing bacterial isolates from clinical samples based on the ability to metabolize D-serine....

20060099613 - Use of antisense oligonucleotide libraries for identifying gene function: A method for identifying one or more genes involved in a phenotype of cells, tissues or organisms, comprising the steps of contacting cells, tissues or organisms which exhibit the phenotype with a library of antisense oligonucleotides and performing a primary phenotypic assay to determine which antisense oligonucleotides in the library...

20060099646 - Bret assay: An improved BRET assay, wherein the BRET signal is enhanced and/or prolonged. The improved BRET assay comprises the steps of i) adding a substrate to a cell comprising GPCR-Rluc fusion protein and a β-arrestin-GFP fusion protein, wherein the (β-arrestin is mutated, ii) adding a ligand to obtain, if possible, a...

20060099648 - Compositions and methods for epitope mapping: The invention provides a composition comprising a diverse population of reagent ligands attached to a solid support and a diverse population of reagent antibodies specifically bound to the reagent ligands. The ligands can be peptides, oligosaccharides, oligonucleotides, or organic molecules. The invention additionally provides methods of determining an epitope in...

20060099649 - Method and apparatus for assay for multiple analytes: A method and apparatus for assay of multiple analytes. The method uses a sensing element comprising a substrate upon which is arranged a multiplicity of recognition elements, such that each element is laid out in a predetermined pattern. Each pattern is unique in that it can give rise to a...

20060099643 - Method for determining the regulation of xenobiotic removal: The present invention relates generally to a method for identifying ligands that affect xenobiotic removal based upon their ability to modify the stability of receptors that regulate cytochrome P450 expression and/or drug transport proteins....

20060099644 - Method of assaying oxidized ldl-beta 2-glycoprotein i complexin vivo: A complex having oxLDL bound covalently to β2-GPI can be used as a standard for measuring a β2-GPI/oxLDL complex in the living body thereby measuring the β2-GPI/oxLDL complex in the living body more accurately and strictly, and can be utilized to provide a new measurement method, detection method, kit etc....

20060099641 - Method of making libraries and anti-ligands: The invention relates to methods of making anti-ligand libraries, which anti-ligands have a cavity binding site. The method comprises providing a plurality of anti-ligands having a cavity for binding a ligand, said plurality of anti-ligands being derived from a first anti-ligand having a cavity for binding a first ligand and...

20060099645 - Methods and reagents for assaying molecular interactions: The invention relates to methods and reagents for screening, identifying, and/or quantifying molecular interactions. In particular, the invention provides a method for identifying protein-protein interactions comprising prey proteins interacting with bait proteins comprising: (a) introducing one or more prey protein in cells, wherein a prey protein is labelled with an...

20060099647 - Methods of identifying kinetically stable proteins: The present invention provides a fast and efficient means for identifying kinetically stable proteins. As used herein the term “kinetically stable protein” means a protein that is trapped in a specific conformation due to an unusually high unfolding barrier that results in very slow unfolding rates. The present inventors are...

20060099642 - Solid-phase substrate for immobilizing biomolecules: The invention relates to a solid phase substrate comprising at least one binding area, which is suited for immobilizing biomolecules. The solid phase substrate is characterized in that the substrate has, in the binding area, reactive binding sites at which polyol prefabricated by means of covalent bonds is immobilized....

20060099650 - Toll-like receptor assays: Methods of identifying compounds that modulate the interaction between a TLR and a molecule that interacts with the TLR by direct binding or by inclusion in a complex that associates with the TLR are described. Methods of identifying molecules that interact with a TLR are also described....

20060099651 - Enriched central nervous system stem cell and progenitor cell populations, and methods for identifying, isolating and enriching for such populations: Enriched neural stem and progenitor cell populations, and methods for identifying, isolating and enriching for neural stem cells using reagents that bind to cell surface markers are provided....

20060099652 - Il 13 receptor alpha 2 antibody and methods of use: The invention provides an antibody directed against an IL13 receptor alpha 2 (IL13-Rα2) and methods of using the antibody to detect and localize IL13-Rα2, to diagnose a disease characterized by expression of IL13-Rα2, and to kill a cell that expresses an IL13-Rα2....

20060099653 - Microscopic precision construction of tissue array block related application data: This present invention covers novel means, devices and instruments for production of a tissue array block that is further sectioned into duplicates of tissue arrays. An integral microscope is incorporated into the instrument for viewing and examining a stained reference slide and selecting donor tissue core region(s) from the reference...

20060099656 - Methods and compositions for treating hematological disorders using 232, 2059, 10630, 12848, 13875, 14395, 14618, 17692, 58874, 252, 304, 1980, 14717, 9941, 1941, 19310, or 17832: The present invention relates to methods for the diagnosis and treatment of hematological disorders. Specifically, the present invention identifies the differential expression of 232, 2059, 10630, 12848, 13875, 14395, 14618, 17692, 58874, 252, 304, 1980, 14717, 9941, 19310, or 17832 genes in tissues relating to hematological disorders sensation, relative to...

20060099657 - Antibodies to a polypeptide encoded by a nucleic acid underexpressed in melanoma: The present invention is directed to novel polypeptides and to nucleic acid molecules encoding those polypeptides. Also provided herein are vectors and host cells comprising those nucleic acid sequences, chimeric polypeptide molecules comprising the polypeptides of the present invention fused to heterologous polypeptide sequences, antibodies which bind to the polypeptides...

20060099659 - Method to detect ige: The present invention includes a method to detect IgE using a human Fc epsilon receptor (FcεR) to detect IgE antibodies in a biological sample from a cat, a dog, or a horse. The present invention also relates to kits to perform such methods....

20060099658 - Pca3, pca3 genes, and methods of use: The present invention relates, in general, to a prostate-specific antigen, PCA3. In particular, the present invention relates to nucleic acid molecules coding for the PCA3 protein; purified PCA3 proteins and polypeptides; recombinant nucleic acid molecules; cells containing the recombinant nucleic acid molecules; antibodies having binding affinity specifically to PCA3 proteins...

20060099660 - Conjugates of biologically active compounds, methods for their preparation and use, formulation and pharmaceutical applications thereof: This invention features a method of identifying a compound useful for enhancing efficacy of a therapeutic agent. The method includes incubating a compound in blood cells; separating immune cells from erythrocytic cells; and determining the ratio of the concentration of the compound in the immune cells to the concentration of...

20060099661 - Detection and quantification of intracellular pathogens: The present invention provides methods and compositions for detecting bacteria of the Chlamydiaceae family in a biological sample. Methods include, for example, contacting a biological sample with an antibody that specifically binds to a chlamydial antigen displayed on the surface of a chlamydia-infected blood cell; and analyzing the sample using...

20060099654 - Methods and kits for the determination of sirolimus in a sample: Methods and kits for determining sirolimus in a sample known or suspected to contain sirolimus are provided. The methods and kits according to the invention use one or more antibodies generated using a fragment of sirolimus, such as the northern fragment of sirolimus....

20060099655 - Human cytoplasmic polyadenylation element binding protein and uses thereof: The present invention provides genomic and cDNA encoding human cytoplasmic polyadenylation element binding protein, expression vectors comprising human cytoplasmic polyadenylation element binding protein cDNA and host cells that contain the expression vectors. Also provided are recombinant human cytoplasmic polyadenylation element binding protein and polypeptides derived thereof. In addition, the present...

20060099662 - Assay for antibodies: The presence and quantity of an antibody of interest in a patient's bloodstream or other biological sample can serve as an important clinical or other analytical or diagnostic tool. ELISA methods, and kits for such assays, as well as anti-idiotypic antibodies and hybridomas producing them, are developed to detect levels...

20060099663 - Peptide sequence tags and method of using same: Peptide sequence tags are identified and used to produce a class of global antibodies, which recognize all members of RbcL protein family with uniform specificity, regardless of the species of origin. The tags are used to create antibodies to the major RbcL protein of the RUBISCO enzyme catalyzing of carbon...

20060099664 - Simultaneous screening of multiple analytes: Methods, compositions and kits are disclosed. The methods are directed to determining the presence of one or more analytes in a sample suspected of containing any one of a plurality of the analytes. A combination is provided comprising in a medium (i) the sample, (ii) a binding partner for each...

20060099665 - Taxol immunoassay: Novel conjugates of taxol and novel taxol immunogens derived from the 9 and 7 positions of taxol and monoclonal antibodies generated by these taxol linked immunogens are useful in immunoassays for the quantification and monitoring of taxol in biological fluids....

20060099666 - Analyzer for the simultaneous enzymatic detection of closely related analytes: An analyzer and a method for simultaneously detecting and measuring the concentration of two related analytes, the analytes being substrates for a common enzyme. The analyzer comprises an enzymatic reaction monitoring component including a support base, a mixed electrode system consisting of a working electrode, an auxiliary electrode and a...

20060099668 - Assay for inhibitors of isph: Assay for testing a sample for the presence or absence of inhibition of the enzymatic conversion of 1-hydroxy-2-methyl-(E)-butenyl 4-diphosphate into isopentenyl diphosphate and/or dimethylallyl diphosphate by the following steps: a) reacting an aqueous mixture containing 1-hydroxy-2-methyl-(E)-butenyl 4-diphosphate, a 1-hydroxy-2-methyl-(E)-butenyl 4-diphosphate reductase, and a reducing agent under predetermined reaction conditions for...

20060099667 - Method for performing restrained dynamics docking of one or multiple substrates on multi-specific enzymes: The present invention relates to a method for performing restrained dynamics docking of one or several substrates having allosteric or synergistic effect on enzymes presenting multipspecific and flexible active site. It also concerns a method for determining the 3D-substrates, which is the case for multispecific enzymes such as cytochrome P450,...

20060099669 - High throughput assay system: A device and a method that permits rapid application of experimental solutions to both (i.e. intracellular and extra-cellular) surfaces of a membrane patch is described. In one embodiment, this is accomplished by mounting a membrane patch on a hole through the side of a hollow tube such that one surface...

20060099670 - Method for the genetic modification of organisms of the genus blakeslea, corresponding organisms and the use of the same: The invention relates to a method for producing a genetically modified organism of the Blakeslea genus, which method comprises the following steps (i) transformation of at least one of the cells, (ii) optional homokaryotic conversion of the cells obtained in step (i) to produce cells, in which one or more...

20060099672 - Activatable recombinant neurotoxins: Compositions comprising activatable recombinant neurotoxins and polypeptides derived therefrom. The invention also comprises nucleic acids encoding such polypeptides, and methods of making such polypeptides and nucleic acids....

20060099671 - Methods and compositions for generating angiostatin: The invention provides methods of generating angiostatin in vitro comprising contacting plasminogen with a plasminogen activator and a sulfhydryl donor or contacting plasmin with a sulfhydryl donor. The invention also provides a method of treating angiogenic diseases by administering to an animal suffering from such a disease a sulfhydryl donor,...

20060099687 - 14926 receptor, a novel g-protein coupled receptor: The present invention relates to a newly identified receptor belonging to the superfamily of G-protein-coupled receptors. The invention also relates to polynucleotides encoding the receptor. The invention further relates to methods using the receptor polypeptides and polynucleotides as a target for diagnosis and treatment in receptor-mediated disorders. The invention further...

20060099674 - Chromatographic purification of recombinant human erythropoietin: The invention provides a method for recovering and purifying recombinant human erythropoietin (rhEpo) from a cell culture medium comprising host cells, which method comprises the steps of: (a) removing host cells, cellular constituents and debris from the cell culture medium by centrifugation using a disc stack separator followed by a...

20060099677 - Dna/rna transduction technology and its clinical and basic applications: This invention relates to a method for in vivo and in vitro transferring efficiently DNA/RNA coding materials regulating bio-function to cytoplasm or nucleus in eukaryotic or prokaryotic cells using PTD (Protein Transduction Domain) and DNA/RNA binding factor. Particularly, this invention provides a method for in vivo transferring the materials to...

20060099686 - Generation of artificial binding proteins on the basis of ubiquitin proteins: The invention relates to modified proteins of the superfamily of “ubiguitin-like proteins”, proteins that have a ubiguitin-like fold and fragments or fusion proteins thereof. As a result of said modification, the proteins have a binding affinity with respect to a predetermined binding partner that did not exist previously. The invention...

20060099681 - Human and mouse alkaline ceramidase 1 and skin diseases: Newly discovered, isolated, and cloned human alkaline ceramidase 1 (haCER1) and mouse alkaline ceramidase 1 (maCER1) are provided, which are predominantly expressed in skin cells and hydrolyze D-erthyro-ceramide exclusively....

20060099682 - Human vanilloid receptors and their uses: The invention provides novel human vanilloid receptor (hVR) proteins, in particular hVR1 and hVR3, nucleotide sequences encoding for the novel hVR proteins, and hVR proteins for use in a method for screening for agents useful in the treatment or prophylaxis of disorders which are responsive to modulation of hVR activity...

20060099683 - Isolated hoat polypeptide: This invention is concerned with human organic anion transporter (“hOAT”). Isolated nucleic acid encoding hOAT is provided, along with ioslated hOAT polypeptide. hOAT nucleic acid and/or hOAT polypeptide are employed in transgenic animals, recombinant cells, replicable vectors and analytical procedures for identifying hOAT agonists or antagonists, assays for identifying hOAT...

20060099676 - Mammalian genes involved in rapamycin resistance and tumorgenesis: rapr6 genes: The invention provides nucleotide sequence of a novel mammalian gene, the RapR6 gene, which is involved in resistance and tumorgenesis, and amino acid sequences of the encoded proteins. The present invention also provides methods and compositions for regulating rapamycin resistance and/or tumorgenesis by modulating the expression and/or the activity of...

20060099675 - Method for preparing an extracellular matrix and its use for tumor cell culture: The invention concerns a method for preparing an isolated extracellular matrix, secreted by tumor cells, in particular epitheliomatous cells, methods for tumor cell culture using such a matrix, the use of such a matrix for producing a tumor cell line as well as novel tumor cell lines obtained by said...

20060099678 - Method of inducing homologous recombination of somatic cell: The present invention provides a method for obtaining diverse novel genes by inducing somatic cell homologous recombination at genetic loci in somatic cells. By controlling transcription activity of a gene that exists at a genetic locus in a eukaryotic organism cell wherein DNA homologous recombination is occurring at an arbitrary...

20060099679 - Methods for engineering polypeptide variants via somatic hypermutation and polypeptides made thereby: Methods using somatic hypermutation (SHM) for producing polypeptide and nucleic acid variants, and nucleic acids encoding such polypeptide variants are disclosed. Such variants may have desired properties. Also disclosed are novel polypeptides, such as improved fluorescent proteins, produced by the novel methods, and nucleic acids, vectors, and host cells comprising...

20060099684 - Novel antiangiogenic peptides: The present invention provides an antiangiogenic polypeptide having the amino acid sequence set forth in SEQ ID NO: 1, or a fragment thereof, which is effective to inhibit endothelial cell proliferation as determined by the capillary EC proliferation assay. Preferably, the fragment has at least 50% inhibition of bFGF-stimulated EC...

20060099673 - Novel recombinant gene expression method by stop codon suppression: This invention describes a novel recombinant gene expression method based on a novel recombinant gene expression vector, comprising in the following order a promoter sequence, a gene of interest, a translational stop signal and translationally linked to said gene of interest a selectable marker gene....

20060099685 - Recombinant expression of factor viii in human cells: The invention discloses a process for recombinant production of blood coagulation Factor VIII in an immortalized human embryonic retina cell, said cell expressing at least an adenoviral E1A protein and comprising a nucleic acid sequence encoding said Factor VIII, said nucleic acid sequence being under control of a heterologous promoter,...

20060099688 - Udp-galactose: beta-d-galactose-r4-alpha-d-galactosyltransferase, alpha4gal-t1: A novel gene defining a novel enzyme UDP-galactose: β-D-galactose-R 4-α-D-galactosyltransferase, termed α4Gal-T1, with unique enzymatic properties is disclosed. The invention provides isolated DNA molecules and DNA constructs encoding α4Gal-T1 and derivatives thereof by way of amino acid deletion, substitution or insertion exhibiting α4Gal-T1 activity, as well as cloning and expression...

20060099680 - Yeast transformant into which genes associated with synthesis system of o-fucosylated protein are introduced: An object of the present invention is to provide a means for producing O-fucosylated protein in large quantities, a means for searching for new genes associated with the synthesis system of O-fucosylated protein or the proteins expressed by the genes, and a means for elucidating the functions thereof. According to...

20060099689 - Use of caspase enzymes for maturation of engineered recombinant polypeptide fusions: The invention relates to methods of producing and isolating recombinant proteins using fusion protein constructs comprising specific recognition sites for a maturating protease. The invention specifically relates to the use of caspase proteases for the maturation of engineered recombinant fusion proteins or polypeptides. These molecules are engineered using recombinant DNA...

20060099690 - Extraction, purification and conversion of flavonoids from plant biomass: A process for preparing a rutin-enriched composition from plant biomass comprises extraction with an aqueous solution, and precipitation. An enzyme preparation, such as naringinase, is used for the transformation of rutin to higher value compositions containing increased proportions of isoquercitrin and quercetin....

20060099691 - Production of complex carbohydrates: Compositions and methods for making complex carbohydrates in a bacterial production cell are disclosed. The complex carbohydrates that can be made include oligosaccharides and polysaccharides of bacterial or mammalian origin....

20060099692 - Microorganisms and processes for enhanced production of pantothenate: The present invention features improved methods for the enhanced production of pantoate and pantothenate utilizing microorganisms having modified pantothenate biosynthetic enzyme activities and having modified methylenetetrahydrofolate (MTF) biosynthetic enzyme activities. In particular, the invention features methods for enhancing production of desired products by increasing levels of a key intermediate, ketopantoate,...

20060099693 - High-quality lipids and methods for producing by enzymatic liberation from biomass: A high-quality lipid composition is disclosed having low oxidative deterioration such as measured by low anisidine values. Also disclosed are methods of preparing the same from a lipid-containing material that include enzymatic degradation of protein and/or carbohydrate components of the material. Lipid-containing materials include biomass, such as microorganisms. The invention...

20060099694 - Process for increasing the yield of lipid and omega-3 fatty acid in seaweed culture: The present invention relates to a new method for producing polyunsaturated fatty acids from algae. The method comprises the step of applying at least growth-limiting factor to an algae culture, causing growth arrest of said algae culture and production and stocking by algae in culture of polyunsaturated fatty acids....

20060099695 - Method of separating poly-3-hydroxyalkanoic acid: The invention aims at providing a method of producing a poly-3-hydroxyalkanoic acid, which comprises carrying out a physical disruption treatment of a suspension of poly-3-hydroxyalkanoic acid-containing microbial cells with adding an alkali thereto either continuously or intermittently and, thereafter, separating the poly-3-hydroxyalkanoic acid....

20060099696 - Method for producing chiral alpha-hydroxycarboxylic crystalline acids: The invention relates to a method for producing chiral α-hydroxycarboxylic crystalline acids consisting in transforming cyanhydrins (R) or (S) into α-hydroxycarboxylic acids (R) or (S), respectively by enzymatic hydrolysis in the presence of Rhodococcus erythropolis NCIMB 11540....

20060099697 - Anaerobic fermenter: The invention relates to a device for anaerobically fermenting substances comprising a preacidifier inside of which the substances are subjected to a preacidification, a fermenter inside of which the preacidified substances ferment, and transfer means for transferring the substances out of the preacidifier and into the fermenter. The device is...

20060099698 - Glucose dehydrogenases: Modified water-soluble glucose dehydrogenase having pyrrolo-quinoline quinone as a coenzyme are provided wherein at least one amino acid residue is replaced by another amino acid residue in a specific region. Modified water-soluble PQQGDHs of the present invention have improved thermal stability....

20060099699 - Udp-galactose:beta-n-acetyl-glucosamine beta-1,3-galactosyl transferases, beta-3gal-t5: A novel gene defining a novel enzyme in the UDP-D-galactose: β-N-acetylglucosamine/β-N-acetylgalactosamine β1,3galactosyltransferase family, termed β3Gal-T5, with unique enzymatic properties is disclosed. The enzymatic activity of β3Gal-T5 is shown to be distinct from that of previously identified enzymes of this gene family. The invention discloses isolated DNA molecules and DNA constructs...

20060099700 - Halohydrin dehalogenases and related polynucleotides: The present invention relates to novel halohydrin dehalogenase polypeptides and the polynucleotides that encode them. These polypeptides are useful in the production of 4-substituted-3-butyric acid derivatives and vicinal cyano, hydroxyl substituted carboxylic acid esters. The invention also provides related vectors, host cells and methods....

20060099701 - Activators of cyclin-dependent kinases: A new gene family involved in cell cycle regulation was discovered. This family has been termed activators of cyclin-dependent kinases (ACDKs). A number of ACDK genes have been cloned and sequenced. Proteins encoded by ACDK genes specifically were shown to interact with CDKs and pRb, and to upregulate the kinase...

20060099702 - Agent-containing bacillus natto culture extract and preservation method of bacillus natto culture extract: To this end, the agent containing Bacillus natto culture extract of the present invention comprises a Bacillus natto culture extract, and any one of a reducing sugar and a reducing sugar derivative. The reducing sugar derivative is preferably the one having no reducing terminal. The invention is particularly effective when...

20060099703 - Device for quantitative analysis of biological materials: Disclosed is a device for quantitatively analyzing material of a living creature. The device includes an analyzing unit using photometric method, and an analyzing unit using electrochemical method. The device has a socket for mounting a photometric test strip and a socket for mounting an electrochemical test strip, separately or...

20060099704 - Method for providing protein microarrays: The present application relates to methods for providing a protein microarray product and related products and services to a customer, methods, kits, and systems for labeling a probe for a protein microarray, and methods for determining protein concentrations using a protein microarray. The methods for providing a protein micorarray can...

20060099705 - Bioreactors with an array of chambers and a common feed line: A bioreactor (200) for cultivating living cells in a liquid medium. In one embodiment, the bioreactor (200) has a substrate (230) having a first surface and an opposite second surface, defining a channel (202) therein, and a plurality of chambers (204) formed in the substrate, wherein each of the plurality...

20060099706 - Exposure device: The present invention relates to an exposure device for living cell cultures, the device having a medium chamber common to a plurality of cell culture chambers and medium directing means. The medium chamber, cell culture chambers and the medium directing means may be arranged so as to provide substantially contemporaneous...

20060099707 - Automated cell preparation system and method: An apparatus and method for automated cell preparation is described. A biological cell sample, including large particles and smaller objects of interest, is introduced into a first chamber. The large particles are trapped in the first chamber using a first filter, while smaller objects-of-interest and small particles pass through the...

20060099708 - Methods for diagnosis and treatment of mdk1 signal transduction disorders: The present invention relates to MDK1 polypeptides, nucleic acids encoding such polypeptides, cells, tissues and animals containing such nucleic acids, antibodies to such polypeptides, assays utilizing such polypeptides, and methods relating to all of the foregoing. Methods for treatment, diagnosis, and screening are provided for diseases or conditions characterized by...

20060099709 - Targeted tumor therapy by use of recombinant adenovirus vectors that selectively replicate in hypoxic regions of tumors: The presently claimed subject matter provides conditionally replication competent adenoviral vectors that confer selective cytotoxicity on cells expressing HIF-1 by infecting cells that allow HIF-1 inducible promoters present within the vectors to function. Also provided are compositions and host cells based upon the vectors, as well as methods of propagating...

20060099710 - Vector for improved in vivo production of proteins: A vector designed to include two tags wherein the first tag improves protein expression, folding and solubility, and the second tag promotes affinity purification, and two distinct recognition sequences for highly specific proteases. Uses of the vector include in vivo protein production....

20060093999 - Adsorbing pathogen-inactivating compounds with porous particles immobilized in a matrix: Methods and devices are provided for reducing the concentration of low molecular weight compounds in a biological composition containing cells while substantially maintaining a desired biological activity of the biological composition. The device comprises highly porous adsorbent particles, and the adsorbent particles are immobilized by an inert matrix. The matrix...

20060094003 - Acetylcholine-dependent current as a novel ionic target for atrial fibrillation: A method for treating a cardiac pathology. The method includes the administration to a mammal of a therapeutically effective amount of a substance interfering with an acetylcholine-dependent potassium current. Also, a method for identifying a compound for treating atrial fibrillation in a mammal....

20060094000 - Destructible surfactants and uses thereof: Destructible surfactants and methods of using same are provided. The invention includes anionic surfactants having a dioxolane or dioxane functional group that enable degradation of the surfactant under acidic conditions. The invention also includes methods of using anionic surfactants in a variety of applications relating to samples containing small molecules....

20060094002 - Eukaryotic biosensor making use of a calcium regulated light emitting enzyme: The present invention provides a method of using eukaryotic cells being transformed with a light emitting Ca2+ regulated photoprotein gene for determining the presence or absence of at least one toxic substance in a sample and for assisting in the identification of the toxicant(s). More specifically there is provided a...

20060094001 - Method and device for image processing and learning with neuronal cultures: It is disclosed a device for image processing and learning comprising at least a “multi electrode array” (MEA), over which an homogeneous culture of interconnected neurons, so that forming a cell network, is grown on, wherein said MEA is able to stimulate and record the electric activity of said neurons....

20060094004 - Micro-reactor, biological material inspection device, and microanalysis system: A micro reactor for inspecting a biological material, comprising: a first substrate on which a minute flow path is formed; a second substrate laminated on the first substrate so as to cover the minute flow path; a detection section provided on the minute flow path formed between the first and...

20060094031 - Alpha-l-arabinofuranosidase histochemical reporter gene assay: The present invention provides a novel reporter gene assay. In particular, the present invention provides a histochemical reporter gene assay employing α-L-arabinofuranosidase in combination with a chromogenic α-L-arabinofuranosidase substrate. The α-L-arabinofuranosidase histochemical reporter assay may be utilized to characterize gene regulatory regions of interest either alone or in combination with...

20060094051 - Apparatus and method for purification of nucleic acids by phase separation using laser and beads: An apparatus and method for purification of nucleic acids of cells or viruses are provided. The nucleic acid purification apparatus includes: a cell lysis capillary having a sample inlet through which samples and magnetic beads are introduced; a vibrator attached to the capillary and mixing the samples and the magnetic...

20060094022 - Array hybridization method including determination of completeness of restriction digest: The present invention provides a method of performing an array hybridization analysis of a sample, including performing a restriction digest reaction on the sample, hybridizing the digested sample to the array, and interrogating the array. The array includes probe sets that provide for a determination of the extent of the...

20060094005 - Capture and detection of target nucleic acid in dipstick assays: Use of helper probes in dipstick assays is described. In a dipstick assay to test for the presence of a target nucleic acid in a sample solution, the sample solution is connected with the contact end of the dipstick to cause the sample solution is contacted with the contact end...

20060094038 - Cardiac pressure overload associated genes: The present invention identifies genes whose gene products are differentially expressed pressure overload of the heart. The invention provides methods for diagnosing or assessing an individual's susceptibility to heart failure from many etiologies, as well as the presence and severity of hypertrophy, chamber enlargement, or systolic heat failure. Also provided...

20060094046 - Compositions and methods relating to angiogenesis and tumorigenesis: Methods for identifying nucleic acid molecules and polypeptides that participate in angiogenesis and tumorigenesis, and associated methods and products are provided....

20060094016 - Determination of methylation of nucleic acid sequences: The invention relates to a method of detecting the precise locations of methyl-cytosines in a given nucleic acid sequence. In particular, the invention features a method which includes sequencing a template nucleic acid that is attached to a hairpin nucleic acid or double-stranded nucleic acid anchor, which contain specifically-designed sites...

20060094039 - Diagnosis of fetal aneuploidy: The invention relates to a method for the early non-invasive diagnosis of fetal aneuploidy. In particular, the invention concerns the diagnosis of fetal aneuploidy by identifying protein expression patterns characteristics of fetal aneuploidy in a maternal biological fluid, such as maternal serum or amniotic fluid....

20060094018 - Discovery and a method for the early detection of pancreatic cancer and other disease conditions: The present invention uses peripheral blood monocyte-lymphocyte for the early diagnosis of pancreatic cancer, as well as other conditions of the pancreas and other organs. The peripheral blood lymphocytes recognize the new neoplasm in the pancreas, as well as disease processes in other organ systems. The evaluation of this specific...

20060094029 - Dre recombinase and recombinase systems employing dre recombinase: The invention provides a Dre/rox recombinase system. In particular, the invention provides Dre polypeptides that can catalyze site-specific recombination at rox sites but not at lox sites. The Dre/rox system can be utilized in a number of genetic manipulations either alone or in combination with other recombinase systems....

20060094024 - Electrochemical arrays: The present invention relates to methods for synthesizing nucleic acids. The invention also relates to the production of an array of nucleic acids as well as methods for making such an array. Electrochemical methods may be used to both fabricate and interrogate the nucleic acid arrays....

20060094054 - Fibulin-3 and uses thereof: Disclosed is a method and test kit to diagnose tumorigenicity or the presence of tumor cells in a patient by detecting the level of FBLN-3 expression or biological activity in the patient. Also disclosed are methods to identify regulators of tumor cell growth, motility and/or invasion, by identifying regulators of...

20060094043 - Genes displaying enhanced expression during cellular senescence and terminal cell differentiation and uses thereof: This invention provides isolated nucleic acid molecules encoding an OLD-35 protein, OLD-64 protein, OLD-137 protein, OLD-139 protein, OLD-142 protein or OLD-175 protein. This invention further provides a purified OLD-35 protein, OLD-64, OLD-137, OLD-139, OLD-142 and OLD-175. Finally, this invention provides different uses of the nucleic acids and proteins....

20060094050 - High fidelity reverse transcriptases and uses thereof: The invention relates to reverse transcriptases which have increased fidelity (or reduced misincorporation rate) and/or terminal deoxynucleotidyl transferase activity. In particular, the invention relates to a method of making such reverse transcriptases by modifying or mutating specified positions in the reverse transcriptases. The invention also relates to nucleic acid molecules...

20060094035 - Identification of tumors: The invention provides methods for the use of gene expression measurements to classify or identify tumors in samples obtained from a subject in a clinical setting, such as in cases of formalin fixed, paraffin embedded (FFPE) samples....

20060094017 - Immunogens for hiv vaccine: Peptidyl sequences, called mimotopes, are disclosed which mimic the binding site of the broadly neutralizing human monoclonal antibody, 2G12, specific for the HIV protein gp120. The mimotopes are identified from a chimeric protein III (pIII) phage display library, each phage containing an additional random 15 amino acids near the N-terminus...

20060094006 - Immunotherapy regimens in hiv-infected patients: This invention relates to an improved method of maintaining an immuno-protective response in persons infected with a retrovirus during early infection or after highly active anti-retroviral therapy....

20060094032 - Irna agents targeting vegf: The features of the present invention relate to compounds, compositions and methods useful for modulating the expression of vascular endothelial growth factor (VEGF), such as by the mechanism of RNA interference (RNAi). The compounds and compositions include iRNA agents that can be unmodified or chemically-modified....

20060094040 - Lithographic mask design and synthesis of diverse probes on a substrate: Systems and methods of synthesizing probes on a substrate are provided. One or more shift reticles are utilized to uniformly add monomers to the substrate at specified locations. The shift reticles are shifted relative to the substrate between monomer addition steps. Additionally, characteristics of the desired probes may be specified...

20060094045 - Macrocyclic chelators for gene-silencing or gene disruption: A composition comprising a kinetically inert ion coordinated to a macrocyclic chelator or ligand, a first functional group bonded to the macrocyclic chelator or ligand, an oligonucleotide, and a second functional group bonded to the oligonucleotide, wherein the first functional group covalently bonds to the second functional group....

20060094011 - Method for altering fatty acid composition of milk: A method of reducing the level of saturated fatty acids relative to the level of unsaturated fatty acids in milk. In particular, the genotyping and/or phenotyping of bovine cows on the basis of the amino acid residue located at position 67 of beta-casein produced in their milk. The invention is...

20060094023 - Method for isolating nucleic acid by using amino surfactants: A method for isolating nucleic acids by forming an insoluble ionic complex between nucleic acids and a surfactant in a biological sample, consisting of a step of contacting the biological sample with an isolation composition comprising amino surfactants of the formula (I): R1R2R3N(O)x, (I), wherein, R1 and R2 each independently...

20060094009 - Method for the characterisation of nucleic acid molecules: A method for characterising nucleic acid molecules comprises the steps of i) introducing a modified base which is a substrate for a DNA endonuclease, for example Endonuclease V from E. coli, into a target DNA molecule; and ii) reacting the nucleic acid containing the modified base with said DNA endonuclease...

20060094021 - Method of determining a chemotherapeutic regimen for non small cell lung cancer based on brca1 expression: The present invention relates to a screening method for classifying patients and for selecting an effective chemotherapy for the treatment of a patient suffering from non-small-cell lung cancer (NSCLC), based on the use of his levels of BRCA1 expression to predict the outcome of chemotherapy....

20060094042 - Method of enhancing fluorescence: A method of enhancing the fluorescence of and/or producing an increased Stokes' shift in a fluorescent dye comprising combining the dye with a base and/or a detergent is described. The method is suitable in chemical or biochemical techniques using fluorescent dyes, particularly techniques requiring staining or labeling of organic molecules,...

20060094007 - Method of identifying pancreatic ductal carcinoma-specific gene using pancreatic ductal cells, method of testing for pdc using said genes, and method of screening pharmaceutical candidate compounds for treating or preventing pdc: A method for identifying a pancreatic ductal carcinoma-specific gene made up of the steps of: a) preparing pancreatic ductal cells from a pancreatic ductal carcinoma patient and a normal individual; b) detecting the gene expression in the pancreatic ductal cells prepared from the pancreatic ductal carcinoma patient and the gene...

20060094010 - Methods and compositions for analyzing comprised sample using single nucleotide polymorphism panels: The present invention provides methods and compositions for analyzing compromised nucleic acid samples. The present invention also includes methods of selecting panels and panels of single nucleotide polymorphisms that are selected so as to be outside of tandem repeat regions, and are not genetically linked....

20060094020 - Methods and compositions for determining resistance or susceptibility of hiv-1 to stavudine: This invention relates to methods for determining resistance or susceptibility of HIV-1 viruses to d4T based on the viral genotypes. The methods generally comprise detecting, in a gene encoding reverse transcriptase of the HIV-1, the presence of a mutation at codon 65 in combination with one or more mutations at...

20060094015 - Methods and materials for using chemical compounds as a tool for nucleic acid storage on media of nucleic acid purification systems: The present invention relates to methods for isolating and storing, nucleic acid from a sample containing nucleic acid, such as a cell sample or cell lysate. The nucleic acid is isolated on a solid phase medium, which is then dried, and which can be stored efficiently, such as at room...

20060094036 - Methods for detection of a target nucleic acid by capture: The invention relates to a method of generating a signal indicative of the presence of a target nucleic acid in a sample, where the method includes forming a cleavage structure by incubating a sample comprising a target nucleic acid with a probe having a secondary structure that changes upon binding...

20060094025 - Methods for detection of microrna molecules: Methods and kits are provided for the production and use in microarray assays of labeled miRNA molecules and labeled cDNA molecules complementary to miRNA molecules....

20060094027 - Multiaxis focusing mechanism for microarray analysis: Systems and methods for positioning a multi-featured biological array relative to a signal acquisition device. Detection of the array's positional deviation may be achieved by a calibration beam reflected from the array surface and detected by a position sensitive detector (PSD). The PSD-measured positional deviation can be transformed and used...

20060094008 - Nucleic acid amplification primers for detecting cytokeratins and examination method with the use of the primers: It is intended to provide novel primers to be used in gene amplification reactions for detecting human cytokeratins (Human CK). A primer containing an oligonucleotide comprising a base sequence which is selected from among the the 270- to 1375-th regions of the base sequence represented by SEQ ID NO:1 and...

20060094026 - Nucleic acid enzyme light-up sensor utilizing invasive dna: The present invention provides a calorimetric light-up sensor for determining the presence and optionally the concentration of an analyte in a sample. Methods of utilizing the sensor and kits that include the sensor also are provided. The sensor utilizes invasive DNA to assist the analyte dependent disaggregation of an aggregate...

20060094014 - Nucleic acid supported protein complementation: The present invention is directed to novel methods for in vitro and in vivo detection of target nucleic acid molecules, including DNA and RNA targets, as well as nucleic acid analogues. The present invention is based on protein complementation, in which two individual polypeptides are inactive. When the two inactive...

20060094047 - Olfactory receptor expression libraries and methods of making and using them: This invention provides novel libraries of olfactory receptor odorant/ligand-binding domains and methods of making and using them. The invention also provides libraries of vectors and cells comprising these nucleic acid constructs. The compositions and methods of the invention are used to identify novel ligand-binding domains for olfactory neuron odorant receptors...

20060094041 - Oligonucleotide and method for detecting verotoxin: An olignucleotide capable of binding to the intramolecular structure-free region of Verotoxin type 1 RNA or Verotoxin type 2 RNA at relatively low and constant temperature, and which can be used in a constant temperature nucleic acid amplification method, is provided. Also, a simple, speedy and highly sensitive method for...

20060094052 - Pcr-based substrate preparation for helicase assays: The present invention relates to a PCR method for generating a labeled double stranded nucleotide sequence that upon digestion with a restriction nuclease generates a double stranded nucleotide sequence having either a 3′ or 5′ overhang of a ssDNA sequence that can serve as helicase substrate....

20060094012 - Polymorphisms for predicting disease and treatment outcome: The invention provides compositions and methods for determining the increased risks for recurrence of certain cancers and the likelihood of successful treatment with one or both of chemotherapy and radiation therapy. The methods comprising determining the type of genomic polymorphism present in a predetermined region of the gene of interest...

20060094019 - Polynucleotide barcoding: A polynucleotide is barcoded using a method whereby an isolated, individual polynucleotide is immobilized on a solid phase and stretched, targets are labeled using target-specific hybridization probes, and an individual label of an unamplified probe at each of the labeled targets is optically detected. The order of the labels is...

20060094037 - Proteins related to schizophrenia and uses thereof: Presenilin Associated Membrane Protein (PAMP), and nucleic acids encoding this protein, are provided. PAMP and PAMP nucleic acids provide diagnostic and therapeutic tools for evaluating and treating or preventing neurodevelopmental and neuropsychiatric disorders. In a specific embodiment, mutations in PAMP are diagnostic for schizophrenia. The invention further relates to screening,...

20060094028 - Rapid diagnostic assay: Disclosed is a rapid and easy to use diagnostic tool that a point-of-care practitioner can use to specifically identify the cause of a disease, such as the upper respiratory infection (URI) pharyngitis. Such a disease has multiple potential causative pathogens and has a number of combined clinical manifestations. The diagnostic...

20060094055 - Rna silencing in animals as an antiviral defense: The present invention provides recombinant DNA constructs for inactivation of viral or endogenous genes in a cell, wherein the construct comprises viral sequence sufficient to activate RNA silencing. In another aspect, the invention provides methods for identifying RNA silencing suppressors by sequence analysis and functional tests. In yet another aspect,...

20060094013 - Salt-inducible kinases 2 and use thereof: The polypeptide of the present invention has the amino acid sequence represented by SEQ ID NO: 2, 4, 6, 8, 10, or 12. The polypeptide of the present invention is useful as an agent for preventing or improving a disease with which the metabolic disorder of fat cells or the...

20060094033 - Screening methods and libraries of trace amounts of dna from uncultivated microorganisms: The invention provides methods for making a gene library from trace amounts of DNA derived from a plurality of species of organisms comprising obtaining trace amounts of cDNA, gDNA, or genomic DNA fragments from a plurality of species of organisms, amplifying the DNA so obtained, and ligating the DNA to...

20060094053 - Self-assembled arrays of lipid-bilayer vesicles: High-density arrays of attoliter volume elements can be created within minutes in a parallel and effortless manner by using self-assembly of nanometer-sized components (e.g., lipid vesicles containing (bio)chemicals) based on biological recognition. The ultrasmall volumes allow localization to a predefined position of a few or single molecules, and then screening...

20060094049 - Stabilized viral envelope proteins and uses thereof: This invention provides an isolated nucleic acid which comprises a nucleotide segment having a sequence encoding a viral envelope protein comprising a viral surface protein and a corresponding viral transmembrane protein wherein the viral envelope protein contains one or more mutations in amino acid sequence that enhance the stability of...

20060094044 - System and method for mild cognitive impairment class discovery using gene expression data: A system and method for mild cognitive impairment (MCI) class discovery using gene expression data are provided. The method comprises: acquiring gene expression data of a patient having MCI; and identifying a putative MCI subtype based on an expression signature in the gene expression data, wherein the putative MCI subtype...

20060094048 - System, method, and product for multiple wavelength detection using single source excitation: An embodiment of a method for adjusting system gain of a biological probe array scanner for a plurality of fluorophore species is described that comprises setting an excitation beam comprising an excitation wavelength at a first power level that elicits an optimal signal to noise ratio response from a first...

20060094034 - Virulence and antibiotic resistance array and uses thereof: An array of nucleic acid probes is described for simultaneously identifying or characterizing a pathotype of a microorganism and detecting antibiotic resistance of said microorganism. Methods are also described for detecting the presence of a microorganism in a sample, as well as determining its pathotype and its antibiotic resistance, using...

20060094030 - Virus like particles, their preparation and their use preferably in pharmaceutical screening and functional genomics: The invention relates to virus like particles, their preparation and their use in pharmaceutical screening and functional genomics. The invention further provides a variety of assay formats to be used with said virus like particles....

20060094057 - Method for automated, large-scale measurement of the molecular flux rates of the proteome or the organeome using mass spectrometry: Disclosed here is a method for measuring the kinetics (i.e., the molecular flux rates—synthesis and breakdown or removal rates) of a plurality of proteins or organic metabolites inn living systems. The methods may be accomplished in a high-throughput, large-scale automated manner, by using existing mass spectrometric profiling techniques and art...

20060094056 - Method of using cytokine assays to diagnose treat, and evaluate inflammatory and autoimmune diseases: The invention provides methods for diagnosing, treating, or evaluating inflammatory and autoimmune diseases by sampling peripheral blood, serum, plasma, tissue, cerebrospinal fluid, or other bodily fluids from a human subject having a suspected diagnosis. The sample is analyzed for the presence and amount of certain cytokines, which provides the diagnosis,...

20060094059 - Methods for identifying new drug leads and new therapeutic uses for known drugs: The screening system utilizes dynamic measurements of pathway activity to detect the activities of drugs within cellular pathways. The methods of the invention can be used to identify previously unknown drug activities and therapeutic uses, even for drugs that have been well characterized with standard biochemical assays. We demonstrated the...

20060094063 - Modulators of retinol-retinol binding protein (rbp)-transthyretin (ttr) complex formation: Described herein are methods and compositions for the detection of transthyretin (TTR), retinol binding protein (RBP) and retinol complex formation. The methods and compositions described herein also provide for the screening of modulators of retinol-RBP-TTR complex formation. Furthermore, the methods and compositions provide for therapeutic agents for the treatment and/or...

20060094061 - Molecular targets and compounds, and methods to identify the same, useful in the treatment of joint degenerative and inflammatory diseases: The application discloses methods for identifying and using compounds that inhibit extra-cellular matrix (ECM) degradation and inflammation, using a polypeptide sequence including SEQ ID NO: 17-127 (hereinafter “TARGETS”) and fragments thereof, expression inhibitory agents such as antisense polynucleotide, a ribozyme, and a small interfering RNA (siRNA), comprising a nucleic acid...

20060094058 - Peptide sequence tags and method of using same: Peptide sequence tags are identified and used to produce a class of global antibodies, which recognize all members of the GlnA protein sub-family with uniform specificity, regardless of the species of origin. The tags are used to create antibodies to the major GlnA protein of the Glutamine Synthetase enzyme catalyzing...

20060094060 - Preparation and use of a reactive solid support surface: A method of preparing a protein-resistant reactive solid support surface is disclosed. The method comprises the steps of providing a solid support having a hydrogel coating with a plurality of binding elements, coupling a protein resistant compound to the hydrogel via a first fraction of the binding elements, and coupling...

20060094062 - Ultra high throughput capture lift screening methods: The present invention relates to a method for identification and isolation of binding molecules having a selective affinity for a ligand. More specifically, this invention provides a process for the ultra high throughput screening of binding molecules from expression libraries containing billions of independent clones without the biases and limitations...

20060094064 - Methods and compositions for diagnosis, stratification, and monitoring of alzheimer's disease and other neurological disorders in body fluids: The inventors have discovered a collection of proteinaceous biomarkers (“AD biomarkers) which can be measured in peripheral biological fluid samples to aid in the diagnosis of neurodegenerative disorders, particularly Alzheimer's disease and mild cognitive impairment (MCI). The invention further provides methods of identifying candidate agents for the treatment of Alzheimer's...

20060094066 - Myeloperoxidase detection in diagnosis and prognosis of hematopoietic disorders: The present invention relates to the diagnosis of hematopoietic disorders and to determining the prognosis of patients affected by such disorders. The methods generally comprise determining a level of myeloperoxidase in a body fluid sample from the individual and using the level as a factor for diagnosing the disorder in...

20060094067 - Method for the determination of characteristics and/or the classification of circulating macrophages, and analysis arrangement for carrying out said method: The invention relates to a method and an analysis arrangement for the determination of characteristics and/or classification of circulating macrophages. A whole blood sample is subjected to a gradient centrifugation for isolating macrophages. The macrophage cells are then perforated and provided with an intracellular staining with at least one selected...

20060094068 - Predictive markers in cancer therapy: Molecular markers useful in medicine response tests are provided, as an aid in determining whether an individual subject s tumor is responding to treatment with EGF and/or erbB2 inhibitors. Markers include phosphorylated ERK protein...

20060094069 - Tumour marker proteins and uses thereof: The invention relates to tumour marker proteins and their preparation from fluids from one or more cancer patients, wherein said fluids are those which collect in a body cavity or space which is naturally occurring or which is the result of cancer or medical intervention for cancer. The invention also...

20060094070 - Trl1 as an antifungal target: The invention provides TRNA ligase (TRL1) as a novel antifungal target, screening methods for TRL1 inhibitors and their use as antifungal compounds, pharmaceutical compositions containing them and their use in medicine, specifically in the treatment of an individual susceptible to or suffering from an anti-fungal infection. In particular the compounds...

20060094065 - Methods of analyzing a sample by maldi-mass spectrometry: Methods of analyzing a sample by MALDI-mass spectrometry are disclosed....

20060094071 - Method for enriching and tracking pathologic modified prions-proteins(prpsc): The invention relates to a method for enriching and tracking pathologic modified prions-proteins (PrPsc) of living organisms....

20060094072 - Peptide sequence tags and method of using same: The presence of a member of a protein in a nitrogenase enzyme system is detected by obtaining a sequence tag selected form the group consisting of VESGGPEPGVGC, VSQSLGHHIAND, CTTCMAEVIGDDL and CMAEVIGDDL, utilizing the tag to elicit the production of antibodies, and using the antibodies to measure the concentration of the...

20060094073 - Saliva immunoassay for detection of antibodies for cardiovascular disease: A method for diagnosing the likelihood and severity of cardiovascular disease in a patient is disclosed. The method determines the levels of antibodies against autoantigens, including myosin, oxidized LDL, β-2-glycoprotein, heat shock protein-60, platelet glycoprotein, and immune complexes. It then compares the results to normal levels to determine the likelihood...

20060094074 - Methods for screening and therapeutic applications for kinesin modulators: The present invention relates to protein modulators, especially modulators of kinesin bioactivities. The present invention provides methods for screening protein modulators, e.g., kinesin modulators that bind to a region of a kinesin and/or that modulate kinesin bioactivities. The present invention also provide methods and compositions for treatment of disorders mediated...

20060094075 - Methods for indentifying compounds that modulate an enzyme in the coenzyme a biosynthetic pathway in a pathogenic microoganism: A method for the identification and treatment of pathogenic microorganism infections by inhibiting one or more enzymes in a metabolic pathway by inhibiting the conversion of substrate to produce the penultimate or ultimate product particularly by inhibiting the activity of one or more of the enzymes in the pathway, and...

20060094076 - Bacteriophages as selective agents: Compositions containing bacteriophages and methods of using bacteriophages in microorganism detection assays and microbial growth and plating media are disclosed. The lytic ability of these phages to control the growth of non-target populations provides superior sensitivity and specificity to detection assays and reduces false negative and false positive results. The...

20060094077 - Increasing bioavailability of carotenoids: A method of increasing a fraction of free carotenoids in a source of carotenoids in which at least some of the carotenoids are fatty acid esterified carotenoids is disclosed. The method is effected by contacting the source of carotenoids with an effective amount of an esterase under conditions effective in...

20060094082 - Composition of lactoferrin related peptides and uses thereof: The present invention is directed to a composition consisting of a series of novel biologically active 33-mer peptides....

20060094081 - Crystal structure of the c-fms kinase domain: applications and use of heterologous substitutions of kinase insert domains for crystallization: The present invention includes a crystal structure of the kinase domain of c-fms and a methodology to produce diffraction quality crystals of the c-fms kinase domain by heterologous substitution of the kinase insert domain. Also included in the invention is the structure of the c-fms kinase domain in liganded form...

20060094078 - Method of improving the production of biomass or a desired product from a cell: The production of biomass or a desired product from a cell can be improved by inducing conversion of ATP to ADP without primary effects on other cellular metabolites or functions which is achieved by expressing an uncoupled ATPase activity in said cell and incubating the cell with a suitable substrate...

20060094079 - Protein for blocking platelet adhesion: A naturally occurring protein isolated from the saliva of the medicinal leech Hirudo medicinalis is described which strongly binds to collagen thus acting as an inhibitor of natural platelet adhesion to collagen. The protein has a molecular weight of about 12,000, an acidic isoelectric point and contains six cysteins. The...

20060094080 - Trichoderma reesei glucoamylase and homologs thereof: The present invention is related to glucoamylases having at least 80% sequence identity to a Trichoderma glucoamylase having the sequence of SEQ ID NO: 4 and biologically functional fragments thereof. The invention is also related to DNA sequences coding for the glucoamylases, vectors and host cells incorporating the DNA sequences,...

20060094083 - Probiotic microorganisms producing chimeric human growth hormone fused with fc fragment of human igg for oral delivery system and methods for producing them: Accordingly, the invention demonstrates that the chimeric protein for oral delivery system can be absorbed in intestine, and delivery of the chimeric protein by oral route using Lactobacillus has very excellent efficiency in vivo test in rats. Accordingly, the Lactobacillus of the present invention is an excellent deliverer of protein...

20060094084 - Microorganism producing 5'-xanthylic acid: The invention relates to Corynebacterium ammoniagenes CJXSP 0201 KCCM 10448 producing 5′-xanthylic acid. More specifically, the invention relates to Corynebacterium ammoniagenes CJXSP 0201 KCCM 10448 which is a mutant strain of Corynebacterium ammoniagenes KCCM 10340 having a resistance to osmotic pressure. In order to obtain mutant strain having enhanced respiratory...

20060094085 - Process and materials for production of glucosamine: The present invention relates to a method and materials for producing glucosamine by fermentation of a genetically modified microorganism. Included in the present invention are genetically modified microorganisms useful in the present method for producing glucsamine, as well as recombinant nucleic acid molecules and the proteins produces by such recombinant...

20060094088 - Acyltransferase regulation to increase the percent of polyunsaturated fatty acids in total lipids and oils of oleaginous organisms: Methods to increase the percent of polyunsaturated fatty acids (PUFAs) within the total lipids and oils of PUFA-producing oleaginous organisms are provided herein, by regulating the activity of specific acyltransferases. Specifically, since oil biosynthesis is expected to compete with polyunsaturation during oleaginy, it is possible to reduce or inactivate the...

20060094086 - Diacylglycerol acyltransferases for alteration of polyunsaturated fatty acids and oil content in oleaginous organisms: Acyltransferases are provided, suitable for use in the manufacture of microbial oils enriched in omega fatty acids in oleaginous yeast (e.g., Yarrowia lipolytica). Specifically, genes encoding diacylglycerol acyltransferase (DGAT1) have been isolated from Y. lipolytica and Mortierella alpina. These genes encode enzymes that participate in the terminal step in oil...

20060094092 - High arachidonic acid producing strains of yarrowia lipolytica: Engineered strains of the oleaginous yeast Yarrowia lipolytica capable of producing greater than 10% arachidonic acid (ARA, an ω-6 polyunsaturated fatty acid) in the total oil fraction are described. These strains comprise various chimeric genes expressing heterologous desaturases, elongases and acyltransferases, and optionally comprise various native desaturase and acyltransferase knockouts...

20060094087 - Mortierella alpina diacylglycerol acyltransferase for alteration of polyunsaturated fatty acids and oil content in oleaginous organisms: An acyltransferase is provided, suitable for use in the manufacture of microbial oils enriched in omega fatty acids in oleaginous organisms. Specifically, the gene encoding diacylglycerol acyltransferase (DGAT2) has been isolated from Mortierella alpina. This gene encodes an enzyme that participates in the terminal step in oil biosynthesis in fungi...

20060094091 - Mortierella alpina glycerol-3-phosphate o-acyltransferase for alteration of polyunsaturated fatty acids and oil content in oleaginous organisms: Glycerol-3-phosphate o-acyltransferase (GPAT) participates in the first step of oil biosynthesis and is expected to play a key role in altering the quantity of long-chain polyunsaturated fatty acids (PUFAs) produced in oils of oleaginous organisms. The present application provides a nucleic acid fragment isolated from Mortierella alpina encoding a GPAT...

20060094090 - Mortierella alpina lysophosphatidic acid acyltransferase homolog for alteration of polyunsaturated fatty acids and oil content in oleaginous organisms: Lysophosphatidic acid acyltransferase (LPAAT) participates in the second step of oil biosynthesis and is expected to play a key role in altering the quantity of long-chain polyunsaturated fatty acids produced in oils of oleaginous organisms. The present application provides a nucleic acid fragment (identified as “LPAAT2”) isolated from Mortierella alpina...

20060094089 - Process for the heterotrophic production of microbial products with high concentrations of omega-3 highly unsaturated fatty acids: A process for the heterotrophic or predominantly heterotrophic production of whole-celled or extracted microbial products with a high concentration of omega-3 highly unsaturated fatty acids, producible in an aerobic culture under controlled conditions using biologically pure cultures of heterotrophic single-celled fungi microorganisms of the order Thraustochytriales. The harvested whole-cell microbial...

20060094093 - Low ph lactic acid fermentation: A process for producing lactic acid which includes incubating acid-tolerant homolactic bacteria in nutrient medium to produce a fermentation broth with high levels of free lactic acid is provided. An isolated acid-tolerant homolactic bacteria capable of producing high levels of free lactic acid is also provided....

20060094094 - Bacteria strain wenelen dsm 16786, use of said bacteria for leaching of ores or concentrates containing metallic sulfide mineral species and leaching processes based on the use of said bacteria or mixtures that contain said bacteria: The present invention is related to an isolated chemolithotrophic bacterial strain belonging to the specie Acidithiobacillus ferrooxidans, named “Wenelen” and deposited at the Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH—DSMZ under classification number DSM 16786, the use of said bacteria for leaching ores or concentrates containing of metallic sulfide species...

20060094095 - Method and circuit arrangement for treating biomaterial: The invention relates to a method for treating biomaterial using at least one electrical field generated by a first voltage pulse which is terminated once the value for an electrical parameter has exceeded or dropped below a preset limit. After the first voltage pulse has been terminated, it is continued...

20060094096 - Fibrous protein-immobilization systems: The present invention provides a fibrous protein-immobilization system composition comprising a fiber comprising fiber-forming material, and a protein attached to the fiber-forming material....

20060094097 - Highly impact-resistant granules: The present invention provides highly impact-resistant, water-soluble or water dispersible, low-dust granules comprising an active ingredient and methods for obtaining the same....

20060094099 - Inactivated enzyme variants and associated process and reagent system: The present invention relates to oxidoreductase apoenzyme variants which are enzymatically inactive but have coenzyme-binding properties. Further, the present invention relates to DNA sequences encoding these oxidoreductase apoenzyme variants, expression vectors containing such DNA sequences and the use of these oxidoreductase apoenzyme variants in diagnostic applications....

20060094098 - Method for purifying protein and glucose dehydrogenase: A method of purifying a target protein from a solution, in which the target protein containing an electron transfer protein is dissolved, with the use of liquid chromatography. The liquid chromatography is performed by introducing the above-described protein solution into a tank filled with a packing agent, thus bonding the...

20060094100 - Structural models of carnitine acyltransferases and uses thereof: The present invention relates to structural models of carnitine acyltransferases, and, in particular, to models of the reactive sites of these enzymes. It is based, at least in part, on the X-ray crystallographic structures of murine carnitine acetyltransferase (“mCRAT”), both in pure form and in complex with its substrates carnitine...

20060094101 - Mk2 interacting proteins: The present invention relates to uses of proteins that bind MK2 to modulate inflammation. More particularly, the invention relates to uses of proteins that bind MK2 for treating condition that are related to inflammation. The invention is useful for treating inflammatory conditions, particularly those in which a decrease in inflammation...

20060094102 - Ammonium transporter promoter for gene expression in oleaginous yeast: The promoter region associated with the Yarrowia lipolytica ammonium transporter (yat1) gene has been found to be particularly effective for the expression of heterologous genes in oleaginous yeast. The promoter regions of the instant invention have been shown to be advantageously inducible under oleaginous conditions (i.e., nitrogen limitation) and are...

20060094103 - Modified bace: The present invention relates to recombinant human BACE polypeptides. More particularly, the invention relates to recombinant human BACE polypeptides that have a modified amino acid sequence at position 33 of the BACE sequence, as well as to polynucleotides, expression vectors, host cells, and methods for producing the modified recombinant human...

20060094104 - Animal protein-free media for cultivation of cells: The present invention relates to animal protein-free cell culture media comprising polyamines and a plant and/or yeast-derived hydrolysate. The invention also relates to animal protein-free culturing processes, wherein cells can be cultivated, propagated and passaged without adding supplementary animal proteins in the culture medium. These processes are useful in cultivating...

20060094105 - Mixed cell diagnostic systems for detection of respiratory, herpes and enteric viruses: The present invention generally relates to the field of diagnostic microbiology, and, more particularly, to compositions and methods for detecting and differentiating one or more viruses or other intracellular parasites present in a specimen. The present invention also provides compositions and methods to evaluate the susceptibility of organisms to antimicrobial...

20060094106 - Biodegradation of subsurface contaminants by injection of gaseous microbial metabolic inducer: The present invention provides a method and a gaseous composition for bioremediation of soil and groundwater contaminated with organic compounds, including halogenated organic compounds and explosives. The gaseous composition contains (a) at least one gaseous microbial metabolic inducer and (b) a carrier gas. The gaseous composition may also optionally include...

20060094107 - Synergistic bioinoculant composition comprising bacterial strains of accession nos. nrrl b-30486, nrrl b-30487, and nrrl-b 30488 and a method of producing said composition thereof: A synergistic composition useful as bioinoculant includes bacterial strains of accession Nos. NRRL B-30486, NRRL B-30487, and NRRL-B 30488, individually or in all possible combinations, and optionally a carrier, with each of the strains showing plant promoting activity, phytopathogenic fungi controlling activity, abiotic stress conditions tolerating capability, phosphate solubilization capability...

20060094108 - Thermal cycler for microfluidic array assays: A system for thermal cycling a plurality of samples. The system includes a case having a fluid-tight cavity defining an interior volume. A microfluidic array is disposed in the interior volume, the array including a sheet of material having a pair of opposed surfaces, a thickness, and a plurality of...

20060094109 - Device and method for analytical cell imaging: In patients with carcinomas tumor cells are shed into the blood, enumeration and characterization of these cells offers the opportunity to obtain a “real time” biopsy of the tumor and may improve the management of the disease. The frequency of circulating tumor cells is rare (<1 cell/ml) and technology is...

20060094111 - Dna containing variant frt sequences: To provide DNA comprising mutant FRT sequence which causes recombination reaction between two mutant FRT sequences having an identical sequence to each other but does not cause recombination reaction with a wild-type FRT sequence, in the presence of FLP recombinase; and a method for performing high-efficiency, gene insertion or gene...

20060094110 - Use of spliceosome mediated rna trans-splicing for immunotherapy: Methods and compositions for generating novel nucleic acid molecules through targeted spliceosomal mediated trans-splicing that result in expression of an immunogenic polypeptide. The invention includes pre-trans-splicing molecules (PTMs) designed to interact with a target precursor messenger RNA molecule (target pre-mRNA) and mediate a trans-splicing reaction resulting in the generation of...

20060094112 - Biological scaffold: Disclosed in this specification is a cell-scaffold composition that comprised of at least two layers biocompatible material, each of which are coated with a biological sealant and contains different cells. The cell-scaffold composition is located in a bioreactor that contains devices for stimulating each of the cells in each of...

20060094114 - Animal product-free cell culture medial extracts, supplements and culture media supplement systems: Provided are APF plant extracts, APF plant extract cell culture media supplements, APF plant extract cell culture media, and APF plant extract cell culture media kits for the support of primary culture and cultivation of normal cells....

20060094113 - Chemically defined media compositions: Chemically defined media compositions for the culture of eukaryotic cells are disclosed. The compositions are useful for eukaryotic cell culture in perfusion bioreactors and other vessels....

20060094115 - Method for the production of cells with increased development potential: The invention relates to a method for producing cells having an increased development potential from cells, which are removed from an organism, wherein the removed cells are cultivated in vitro, and wherein a native nuclear non-activated MAPKAP kinase of the cultivated cell is activated or wherein an activated MAPKAP kinase...

20060094116 - High-efficiency wild-type-free aav helper functions: The present invention provides methods and compositions for producing high titer, wild-type-free preparations of recombinant AAV (“rAAV”) virions. The compositions of the present invention include novel nucleic acids encoding AAV helper functions and AAV helper function vectors. The present invention also includes host cells transfected by the claimed nucleic acids,...

20060094117 - Plasmid-free clone of e. coli strain dsm 6601: The invention relates to a plasmid-free clone of Escherichia coli strain DSM 6601, to a method for preparing the same and to the use thereof as a cloning vehicle....