Chemistry: molecular biology and microbiology inventions 05/06

20060110721 - Apparatus and method for in vitro storage of a cornea: A fixture and method is provided for in vitro storage of a cornea. The fixture includes a platform having a corneal-sceral rim receiving surface, a clamp having a mating surface for the cornel-scleral rim and handles. A locking mechanism is provided to secure a donor cornea between the clamp and...

20060110720 - Method of treating biological tissue for transplantation by applying ultrahigh hydrostatic pressure: Native tissue of mammalian origin are treated for transplantation by applying ultrahigh hydrostatic pressure to the tissue in a liquid medium. The connective tissue is rendered isolatable from the remainder of the tissue including cellular components while keeping intact the structure and biomechanical properties thereof....

20060110722 - Cell mimic platform and method: A platform and method for mimicking the environment within a cell is provided. The platform includes a microfluidic device defining a chamber. At least one hydrogel post is positioned within the chamber of the microfluidic device. Each hydrogel post defines a corresponding pore for receiving a first molecule therein. Second...

20060110725 - Apparatus for and method of purifying nucleic acids by different laser absorption of beads: An apparatus for and method of purifying nucleic acids of cells or viruses are provided. The nucleic acid purification apparatus includes: a cell lysis capillary having a sample inlet through which samples, magnetic beads, and a solid support are introduced; a vibrator attached to the capillary and mixing the samples,...

20060110723 - Multiplexed protein expression and activity assay: A system is for analyzing expression levels and activity of a plurality of proteins is provided. A bio-displayed polypeptide binding component associated with a predetermined marker is used to bind the proteins of interest. The predetermined marker components are then amplified and detected in a high throughput manner....

20060110726 - Polymerase chain reaction assays for monitoring antiviral therapy and making therapeutic decisions in the treatment of acouired immunodeficiency syndrome: The present invention relates to methods of monitoring, via polymerase chain reaction, the clinical progression of human immunodeficiency virus infection and its response to antiretroviral therapy. According to the invention, polymerase chain reaction assays may be used to predict immunological decline and to identify, at an early stage, patients whose...

20060110724 - Quantitative real-time assay for noroviruses and enteroviruses with built in quality control standard: A method is provided for reverse transcription-polymerase chain reaction (RT-PCR) comprising a) amplifying a reverse transcribed cDNA in a mixture comprising Norovirus Genogroup I and Norovirus Genogroup II primers and probes, wherein said Norovirus primers and probes distinguish between Genogroup I and Genogroup II viruses; b) quantifying virus; and c)...

20060110754 - Amplification and separation of nucleic acid sequences using strand displacement amplification and bioelectronic microchip technology: Described and disclosed are devices, methods, and compositions of matter for the multiplex amplification and analysis of nucleic acid sequences in a sample using novel strand displacement amplification technologies in combination with bioelectronic microchip technology. Specifically, a nucleic acid in a sample is amplified to form amplicons, the amplicons are...

20060110759 - Biomarkers for prostate cancer metastasis: The present invention provides genomic markers for determining the predisposition of prostate cancer to become metastasized....

20060110739 - Biosensors for detecting macromolecules and other analytes: The invention generally provides molecular biosensors. In particular, the invention provides molecular biosensors having one or more aptamers. The molecular biosensors are useful in several methods including in the identification and quantification of target molecules....

20060110731 - Cancer-linked gene as target for chemotherapy: Cancer-linked gene sequences, and derived amino acid sequences, are disclosed along with processes for assaying potential antitumor agents based on their modulation of the expression of these cancer-linked genes. Also disclosed are antibodies that react with the disclosed polypeptides and methods of using the antibodies to treat cancerous conditions, such...

20060110742 - Carrier tests for polycystic kidney disease in the cat: The present invention provides compositions and methods for detecting mutations associated with polycystic kidney disease in the cat....

20060110750 - Cleavage of nucleic acids: The present invention relates to means for the detection and characterization of nucleic acid sequences, as well as variations in nucleic acid sequences. The present invention also relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a...

20060110733 - Coded nucleic acid carriers: The present invention relates generally to coded solid or semi-solid nucleic acid carriers for use in multiplexing solid phase nucleic acid-based reactions. The use of coded carriers facilities multiplexing due to the ability to deconvolute multiple nucleic acid-based events and to correlate those to particular experiments. The present invention further...

20060110770 - Deoxyribonuclease, gene encoding same and use thereof: This invention provides a novel acid DNase (DLAD) which is an endonuclease capable of cleaving DNA independently from divalent cations, under acidic conditions, which retains its activity in acidic to even neutral pH range, and which is not inhibited by G-actin. This invention also provides a DNA encoding the enzyme,...

20060110765 - Detection of nucleic acid variation by cleavage-amplification (cleavamp) method: Methods and compositions for detecting nucleic acid polymorphisms are provided....

20060110734 - Diagnosis of shwachman-diamond syndrome: The SBDS gene has been identified as the site of mutations associated with SDS. Methods are provided for determining whether a subject is suffering from SDS....

20060110743 - Drug evolution: drug design at hot spots: A new method of designing and generating compounds having an increased probability of being drugs, drug candidates, or biologically active compounds, in particular having a therapeutic utility, is disclosed. The method consists of identifying a group of bioactive compounds, preferably of diverse therapeutic uses or biological activities and built on...

20060110767 - Genotyping assay to predict cytochrome p4503a5 exression: Genetic polymorphisms responsible or associated with altered expression of cytochrome P450 CYP3A5 enzyme are described. Single nucleotide polymorphisms are provided. Methods for identifying subjects having a low or high drug metabolizing phenotype associated with CYP3A5 expression are provided. Assays, kits and methods for determining and assaying the CYP3A5 genotype and...

20060110736 - Hiv vaccine formulations: Provided herein are HIV vaccines comprising HIV polypeptide-encoding DNA adsorbed to PLG and/or HIV proteins. Also provided are methods of using these vaccines to generate immune responses in a subject....

20060110737 - Human tumor-associated gene ct120 on chromosome 17p 13.3 region and the protein encoded by it: The invention related to a novel human tumor associated polypeptide CT120, and the encoding polynucleotide, and to the method for producing the polypeptide by recombinant technology, also to the method for diagnosing and treating diseases such as carcinoma using the polypeptide. The invention also related to antagonists against the polypeptide...

20060110761 - Identification of a gene causing the most common form of bardet-biedl syndrome and uses thereof: The present invention relates to the identification of a gene, mutated at the most common locus now designated BBS1, that is involved in the genetic disease Bardet Biedl Syndrome (BBS), which is characterized by such diverse symptoms as obesity, diabetes, hypogonadism, mental retardation, renal cancer and other renal abnormalities, retinopathy...

20060110756 - Large-scale parallelized dna sequencing: We provide a DNA sequencing method and a sequencing system where large numbers of sequence reads can be obtained in parallel by running traditional electrophoresis in a special format. Parallelization is obtained either through a 3-dimensional gel-cube or through bundled capillary tubes including fiber-optic tubes or other types of micro...

20060110764 - Large-scale parallelized dna sequencing: We provide a DNA sequencing method and a sequencing system where large numbers of sequence reads can be obtained in parallel by running traditional electrophoresis in a special format. Parallelization is obtained either through a 3-dimensional gel-cube or through bundled capillary tubes including fiber-optic tubes or other types of micro...

20060110751 - Method and kit for detecting a risk of essential arterial hypertension: Genes, SNP markers and haplotypes of susceptibility or predisposition to hypertension (HT) are disclosed. Methods for diagnosis, prediction of clinical course and efficacy of treatments for HT using polymorphisms in the HT risk genes are also disclosed. The genes, gene products and agents of the invention are also useful for...

20060110745 - Method for amplifying nucleic acids: The invention provides a sequence specific method for amplifying nucleic acids. More particularly, the invention provides a method for amplifying nucleic acid sequences which enables such sequences to be detected with high precision, rapidity and high specificity as compared to conventional methods. The present invention also provides a simple method...

20060110768 - Method for determining genotoxicity: Methods and reagents for determining the genotoxicity of a compound based on genomic responses to contact therewith are provided....

20060110732 - Method for the identification of ligands: The present invention relates generally to a method of identifying ligands that modulate protein-protein interactions. More particularly, the present invention relates to methods of determining agonists or antagonists of a co-regulator dependent target molecule based on the ability to modify the stability of the target molecule in a tissue-selective manner....

20060110757 - Method of designing probe from polynucleotide group comprising plurality of polynucleotides: There is provided a method of designing a probe from a polynucleotide group comprising a plurality of polynucleotides, the method including: forming a polynucleotide group by selecting polynucleotides having a certain sequence; producing polynucleotide fragments having a certain length from each of the polynucleotides of the polynucleotide group and obtaining...

20060110766 - Method of determining a cellular response to a biological agent: A method of determining a cellular response to a biological agent can be preformed in the presence of gene silencing. Such a method can include the use of siRNA in order to silence various genes in the cell in order to monitor the cellular response to the biological agent when...

20060110729 - Method of dna testing for mycobacterium paratuberculosis strains: The present invention relates to the discovery of a DNA sequence in sheep types of M. paratuberculosis that differs from the homologous sequence in cattle types of M. paratuberculosis. The invention also provides a nucleic acid amplification technique based on these differences that can be used to distinguish strains of...

20060110730 - Methods and compositions for regulation and manipulation of steroidogenesis: The steroidogenic acute regulatory protein (StAR), a mitochondrial protein required for stress responses, reproduction, and sexual differentiation of male fetuses is shown to exert its activity transiently at the outer mitochondrial membrane (OMM) rather than in its final resting place in the matrix, and that its OMM residency time and...

20060110748 - Methods for detection of a nucleic acid by sequential amplification: The invention relates to nucleic acid, flap-mediated, sequential amplification methods which permit detection of a nucleic acid target in a nucleic acid sample. The invention provides for nucleic acid synthesis dependent, flap-mediated amplification methods for sequentially producing detectable, released flaps to detect a target nucleic acid. The invention provides for...

20060110753 - Methods to diagnose and treat lung cancer: The present invention provides compositions and methods for aiding in the diagnoses of the neoplastic condition of a lung cell, and methods of screening for a potential therapeutic agents for the reversal of the neoplastic condition. Also provided are therapeutic compositions and methods to inhibit the growth of neoplastic lung...

20060110760 - Microarray using laminar flow and method of preparing the same: A microarray including hydrogel and a plurality of probes which are immobilized in discrete regions of the hydrogel, and a method of preparing the same are provided. When using the microarray and method, a solid substrate is not required and many biomolecules can be immobilized in a small volume, thereby...

20060110727 - Modified nucleic acid probes: Oligonucleotide analogue arrays attached to solid substrates and methods related to the use thereof are provided. The oligonucleotide analogues hybridize to nucleic acids with either higher or lower specificity than corresponding unmodified oligonucleotides. Target nucleic acids which comprise nucleotide analogues are bound to oligonucleotide and oligonucleotide analogue arrays....

20060110741 - Nucleic acid methylation detection process using an internal reference sample: There is disclosed a process for detection of DNA methylation at CpG sites using nucleic acid arrays and preferably microarrays. Specifically, there is disclosed a process for directly generating a reference sample from the sample to be tested and detecting methylation at large numbers of CpG island sites simultaneously. More...

20060110763 - Nucleic acid preparation: The present invention is directed to methods, devices and computer programs for preparing nucleic acids from a template nucleic acid by subjecting a sample to thermocycles. After a first number of thermocycles, a partial amount of the reaction mixture is being subjected to a second number of thermocycles. This two...

20060110738 - Polarization-enhanced detector with gold nanorods for detecting nanoscale rotational motion and method therefor: A nanoscale motion detector attaches a gold nanorod (30) to the rotating arm (26) of a molecular structure (10) to cause the nanoparticle to rotate. The molecular structure is an F1-ATPase enzyme. The gold nanorod is exposed to a light source. The long axis of the gold nanorod scatters red...

20060110752 - Polymorphism detection: The present invention generally provides a rapid efficient method for analyzing polymorphic or biallelic markers, and arrays for carrying out these analyses. In general, the methods of the present invention employ arrays of oligonucleotide probes that are complementary to target nucleic acids which correspond to the marker sequences of an...

20060110762 - Porcine reproductive and respiratory syndrome virus receptor components and uses thereof: This invention relates to host cellular receptor components for Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) and methods of their use in diagnosis, prevention, control, and treatment of PRRSV disease in animals....

20060110749 - Primer composition and method of using the same in the detection of shigella sonnei: The invention provides a new primer composition for detecting the presence of Shigella sonnei and a method of using the same. The primer composition and method have high specificity and sensitivity on the detection of Shigella sonnei. The invention also provides a method for extracting the nucleic acids of microorganisms...

20060110744 - Probe design methods and microarrays for comparative genomic hybridization and location analysis: Methods and systems for identifying and selecting nucleic acid probes for detecting a target with a nucleic acid probe array or comparative genome hybridization microarray, comprising selecting a plurality of potential target sequences, generating a plurality of candidate probes from the target sequences, filtering the plurality of candidate probes by...

20060110747 - Process for improved protein expression by strain engineering: This invention is a process for improving the production levels of recombinant proteins or peptides or improving the level of active recombinant proteins or peptides expressed in host cells. The invention is a process of comparing two genetic profiles of a cell that expresses a recombinant protein and modifying the...

20060110769 - Regulation of novel human prolyl 4-hydroxylases: Reagents that regulate human prolyl 4-hydroxylase and reagents which bind to human prolyl 4-hydroxylase gene products can play a role in preventing, ameliorating, or correcting dysfunctions or diseases including, but not limited to cardiovascular disorders, cancer, inflammatory diseases, fibrotic disorders, anemia, and CNS disorders, such as stroke....

20060110728 - Supported double layer structure for displaying a nucleic acid associated with a protein: The invention concerns novel biosensors, in particular a support for displaying nucleic acids and for detecting both the presence of nucleic acids in a sample and the linkage between proteins and nucleic acids, as well as the linkage between a ligand and a protein linked to a nucleic acid....

20060110758 - Synthetic peptide targeting critical sites on the sars-associated coronavirus spike protein responsible for viral infection and method of use thereof: The present invention provides methods for locating critical portions or sites on the spike protein (S protein) of SARS-associated coronavirus (SARS-CoV) responsible for the viral infection that causes Severe Acute Respiratory Syndrome (SARS). The present invention also provides new synthetic peptides targeting such critical portions or sites of the S...

20060110746 - Treatment of cancer using tlr3 agonists: The present invention relates generally to the fields of genetics and medicine. More specifically, the present invention relates to improved methods of treating cancers using a TLR3 agonist, by assessing the expression of a TLR3 receptor by cancer cells....

20060110735 - Use of molecular markers for the preclinical and clinical profiling of inhibitors of enzymes having histone deacetylase activity: The present invention relates to the use of molecular markers and related signaling mechanisms for the preclinical and clinical profiling of inhibitors of enzymes having histone deacetylase activity. The invention also relates to the use of such markers as diagnostic and/or prognostic tools for the treatment of tumor patients with...

20060110740 - Use of sendai virus as a human parainfluenza vaccine: Immunogenic compositions to protect against human parainfluenza (hPIV) virus infection are provided. The compositions comprise an unmodified Sendai virus that can be safely administered to human subjects, particularly young children, to protect against symptoms and health risks associated with hPIV infection....

20060110755 - Yeast-based therapeutic for chronic hepatitis c infection: Disclosed are compositions, including vaccines, and methods for vaccinating an animal against hepatitis C virus (HCV) and for treating or preventing hepatitis C viral infection in an animal. The invention includes a variety of novel HCV fusion proteins that can be used directly as a vaccine or in conjunction with...

20060110772 - Human and murine cytokine polypeptides: This invention relates to IMX7189 cytokines and to new members of the human cytokine polypeptide family, methods of making such polypeptides, and to methods of using them to treat conditions and diseases involving proliferation and/or differentiation of cells from pluripotent stem cell precursors, and to identify compounds that alter cytokine...

20060110773 - Marker for inflammatory conditions: Use of pregnancy-associated plasma protein-A as a marker for inflammatory conditions, and in particular, for acute coronary syndromes is described....

20060110775 - Method of detecting native probnp: The present invention relates to antibodies specifically binding to native proBNP, a method for specific detection of native proBNP, a method of correlating the level of native proBNP to the diagnosis of heart failure, a kit for detection of native proBNP and to a hybridoma cell line producing an antibody...

20060110776 - Method of detecting native probnp: The present invention relates to antibodies specifically binding to native proBNP, a method for specific detection of native proBNP, a method of correlating the level of native proBNP to the diagnosis of heart failure, a kit for detection of native proBNP and to a hybridoma cell line producing an antibody...

20060110774 - Methods for detecting sepsis: Compositions and methods for detecting sepsis by contacting a subject-derived sample with a ligand that binds to GRK and determining the concentration of GRK in the sample. An increase in the concentration of GRK compared to that of a normal, healthy control sample indicates that the subject from which the...

20060110771 - Proteins capable of binding to female sex hormones and process for producing the same: The present invention provides a protein capable of binding to a female sex hormone, which protein is supplemented with useful properties for measuring, quantifying and concentrating female sex hormones, such as high sensitivity, low cross-reactivity, unlikelihood of being influenced by interfering substances, and unlikelihood of being influenced by solvents. Specifically,...

20060110777 - Structured peptide scaffold for displaying turn libraries on phage: The invention is directed to a model system for structure-activity analysis of peptide or protein molecules involved in important biological processes. Provided by the invention are combinatorial peptide libraries comprising disulfide-constrained cyclic peptides with sequences favorable for energy stabilized conformations. One aspect of the invention is directed to cyclic peptide...

20060110778 - High-throughput screen for identifying selective persistent sodium channels channel blockers: A method for identifying a selective persistent Na+ channel blocker by measuring the ability of the blocker to reduce or inhibit a persistent Na+ current to a greater degree than a transient Na+ current. Aspects of the present method provide Na+ depletion/repletion methods for identifying a selective blocker of a...

20060110779 - Method for detecting a lectin-like substance: The present invention provides a method for detecting lectin-like substances, which comprises the steps of (a) providing a first carbohydrate molecule and a test target, wherein the first carbohydrate molecule specifically binds to the lectin-like substance to be detected; (b) contacting the test target with the first carbohydrate molecule so...

20060110780 - Plasma c5a levels as an indicator of asthma severity: This invention provides the first blood test which characterizes asthma severity. More specifically, the present invention provides methods of determining the severity of acute asthma in a patient by determining the levels of C5a or C5a-desArg in the patient's blood, plasma or serum....

20060110781 - Cholesterol detection reagent: An object of the present invention is to provide a novel cholesterol detection reagent comprising a substance which can specifically binds to cholesterol to detect it, and a method for detecting cholesterol using the reagent. The present invention provides a cholesterol detection reagent comprising a polyethylene glycol cholesteryl ether which...

20060110782 - Compositions and methods for modification of biomolecules: The present invention provides modified cycloalkyne compounds; and method of use of such compounds in modifying biomolecules. The present invention features a cycloaddition reaction that can be carried out under physiological conditions. In general, the invention involves reacting a modified cycloalkyne with an azide moiety on a target biomolecule, generating...

20060110783 - Diagnostics and therapeutics for diseases associated with human phosphodiesterase 10a (pde10a): The invention provides a human PDE10A which is associated with the disorders of the peripheral and central nervous system (e.g. Alzheimer), cardiovascular diseases, cancer (e.g. thyroid, oesophagus, stomach, liver, ovary, kidney), gastroenterological diseases (e.g. liver cirrhosis), endocrinological diseases and urological diseases (e.g. benign prostate hyperplasia). The invention also provides assays...

20060110785 - Methods to differentiate protein conformers: The invention is directed to methods to distinguish among different protein conformers of the same protein such as proteins which form amyloid deposits. Using the methods of the invention, one or more protein conformers in a sample can be detected, differentiated, and quantitated. An example of a protein which is...

20060110784 - Site-specific labeling of proteins for nmr studies: Methods of producing and/or analyzing spectroscopically labeled proteins, e.g., proteins site-specifically labeled with NMR active isotopes, spin-labels, chelators for paramagnetic metals, and the like, are provided. The labeled proteins are produced in translation systems including orthogonal aminoacyl tRNA synthetase/tRNA pairs. Methods for assigning NMR resonances, e.g., methods using isotopically labeled...

20060110786 - Compositions and methods for detecting and quantifying cox-2 activity and 2-arachidonylgycerol metabolites: The present invention provides methods, compositions and kits for discriminating between COX-1 and COX-2 activity. In particular, the prevent invention provides for the detection and/or measurement of COX-2 activity in subjects, samples thereof, and in laboratory tests. The present invention discloses that 2-arachidonylglycerol is a COX-2 selective substrate which is...

20060110787 - Use of fluorine-18-labeled fluoroquinolone antibiotics for diagnosing and monitoring bacterial infection: The invention provides methods of diagnosing and imaging bacterial infections in mammals using 18F-labeled fluoroquinolone antibiotics and detecting the fluorine-18. Fluorine-18 can be detected and imaged by positron emission tomography. The invention also provides methods of detecting bacteria in other settings, and of treating a bacterial infection and monitoring treatment...

20060110788 - Feeding buffers, systems, and methods for in vitro synthesis of biomolecules: Compositions, methods and kits for in vitro systems for synthesis of biomolecules such as polypeptides, are provided herein. Cell extracts that provide enhanced yields of soluble proteins using in vitro protein synthesis methods are provided. The invention also includes methods for producing high yields of proteins by the addition of...

20060110798 - Cells expressing recombinase useful for adenovirus vector production and control of gene expression: Embodiments of the invention provide cells that stably express an integrase family recombinase, for example Cre or FLP. Examples of production of such cells, and uses of such cells, are provided. In some embodiments, a cell may additionally express one or more adenovirus E1 proteins. In some embodiments, the cell...

20060110790 - Expression system for large functional proteins: The present invention provides a system and method for expressing functional ABC (ATP-binding cassette) proteins, from the ABCA subfamily, in a host cell. A system comprises two or more expression vectors each comprising a nucleic acid molecule encoding one or more domains of an ABC transporter gene and a means...

20060110791 - Heterologus expression of trypanothione reductase from leishmania donovani in a prokaryotic system: The present invention relates to a process for heterologous expression and large-scale production of functionally active enzyme trypanothione reductase of Leishmania donovani in prokaryotic system....

20060110796 - In vivo site-specific incorporation of n-acetyl-galactosamine amino acids in eubacteria: Methods and compositions for making glycoproteins, both in vitro and in vivo, are provided. One method involves incorporating an unnatural amino acid having a N-acetylgalactosamine moiety into a protein; optionally, the N-acetylgalactosamine-containing unnatural amino acid can be further modified with additional sugars....

20060110793 - Methods and compositions for increasing longevity and protein yield from a cell culture: Disclosed herein are compositions and methods for increasing the longevity of a cell culture and permitting the increased production of proteins, preferably recombinant proteins, such as antibodies, peptides, enzymes, growth factors, interleukins, interferons, hormones, and vaccines. By transfecting cells in culture with an apoptosis-inhibiting gene or vector, cells in culture...

20060110789 - Methods to mediate polyketide synthase module effectiveness: Linking sequence which modulate cross-talk between modules of Type I polyketide synthases have been identified. Thus, arbitrarily chosen modules can be mixed and matched by supplying the appropriate linkers to obtain desired polyketide synthases and new polyketides. The modules are provided suitable linkers so that the polyketide chain is passed...

20060110799 - Mitogen-activated protein kinase kinase mek6 and methods of use thereof: Compositions and methods are provided for potentiating the activity of the mitogen-activated protein kinase p38. In particular the mitogen-activated protein kinase kinase MEK6, and variants thereof that stimulate phosphorylation of p38 are provided. Such compounds may be used, for example, for therapy of diseases associated with the p38 cascade and...

20060110794 - Peptides from the e2 proteins of human papillomaviruses 16 and 18 for detecting and/or diagnosing cervical and other human papillomavirus associated cancers: An isolated protein sequence or peptide from the E2, E6 or E7 early coding region of human papillomavirus (HPV) that is soluble in an aqueous medium, and characterized by a relative paucity of tryptophan, methionine and cysteine residues, and a relative abundance of glycine and asparagine residues. Also disclosed are...

20060110797 - Polypeptides having xyloglucanase activity and nucleic acids encoding same: The present invention relates to isolated polypeptides having Family 74 xyloglucanase activity and isolated nucleic acid sequences encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences as well as methods for producing and using the polypeptides....

20060110792 - Potassium channels, nucleotide sequences encoding them, and methods of using same: This invention relates generally to a new family of potassium channels. More particularly, the present invention relates to the cloning and characterization of a family of distinct trans-membrane potassium ion channels, characterization of such channels, newly identified polynucleotide sequences, polypeptides encoded by such sequences, expression vectors capable of heterologous expression...

20060110795 - Protein secretion in eukaryotic cells: The present invention relates to the use of a glucosidase II mutation to increase protein secretion in eukaryotic cells. The present invention relates further to the use of eukaryotic cells, comprising a mutant glucosidase II gene, possibly in combination with the expression of a recombinant α-1,2-mannosidase gene and/or a recombinant...

20060110800 - Testis-specific receptor: Novel receptor polypeptides, polynucleotides encoding the polypeptides, and related compositions and methods are disclosed. The polypeptides comprise an extracellular domain of a cell-surface receptor that is expressed in testis cells. The polypeptides may be used within methods for detecting ligands that promote the proliferation and/or differentiation of testis cells, and...

20060110801 - Receptor for b. anthracis toxin: The present invention relates to mammalian anthrax toxin receptor polypeptides and polynucleotides encoding same as well as related polypeptides and polynucleotides, vectors containing the polynucleotides and polypeptides, host cells containing related polynucleotide molecules, and cells displaying no anthrax toxin receptor on an exterior surface of the cells-minus cell lines and...

20060110802 - Cytokine designated 4-1bb ligand and human receptor that binds thereto: Novel 4-1BB ligand (4-1BB-L) polypeptides and a human cell surface receptor designated 4-1BB that binds 4-1BB-L are provided. Isolated 4-1BB-L-encoding and human 4-1BB-encoding DNA sequences, recombinant expression vectors comprising the isolated DNA sequences, and host cells transformed with the recombinant expression vectors are disclosed, along with methods for producing the...

20060110803 - Antigenic peptides of sars coronavirus and uses thereof: The present invention pertains to antigenic peptides of SARS-CoV and their use in diagnostic test methods and in the treatment of condition resulting from SRAS-CoV. Furthermore, this invention provides antibodies capable of specifically recognizing the peptides of the invention. The antibodies can also advantageously be used in diagnostic test methods...

20060110804 - Method of treating an organ for transplantation: The present invention provides a method of treating an organ for transplantation, in which an organ graft is cold-preserved with an organ preservation solution for a given period of time and reperfused with a solution containing an intracellular accessibility, lipophilic small-molecular antioxidant (e.g., bilirubin) to wash the organ graft before...

20060110805 - Microbial production of xylitol via hexose phosphate and pentose phosphate intermediate: The invention provides methods and compositions for production of xylitol, xylose, or combinations thereof from simple sugars from biosynthetic pathways that utilize D-fructose-6-phosphate and D-xylulose-5-phosphate as pathway intermediates....

20060110806 - Docosahexaenoic acid producing strains of yarrowia lipolytica: An engineered strain of the oleaginous yeast Yarrowia lipolytica capable of producing greater than 5.6% docosahexaenoic acid acid (DHA, an w-3 polyunsaturated fatty acid) in the total oil fraction is described. This strain comprises various chimeric genes expressing heterologous desaturases, elongases and acyltransferases and optionally comprises various native desaturase and...

20060110807 - Process for the preparation of phenolic carboxylic acid derivaties by enzymatic catalysis: The present invention relates to an improved process for the preparation of phenolic carboxylic acid derivatives catalysed by biocatalytic esterification, transesterification or amidation of a corresponding lower alkyl ester. Biocatalysis is performed in the presence of suitable enzymes, e.g. hydrolases, especially esterases, amidases, lipases and proteases....

20060110808 - Methods for preserving and/or storing cellshaving a nitrilase or nitrile hydratase avtivity: The invention relates to a method for preserving and/or storing microorganisms which exhibit at least one nitrile hydratase or nitrilase enzyme activity, with the preservation and/or storage being effected in an aqueous medium which comprises at least one aldehyde, with the total aldehyde concentration being in a range from 0.1...

20060110809 - Methods for production of xylitol in microorganisms: The invention provides biosynthetic routes to xylitol production that do not require pure D-xylose for synthesis and that can utilize inexpensive substrates such as hemicellulose hydrolysates....

20060110810 - Methods and materials for the synthesis of organic products: This invention provides biocatalysts that are recombinant yeast cells comprising recombinant expression vectors encoding heterologous lactate dehydrogenase genes for producing lactate....

20060110811 - Novel candida tropicalis cj-fid(kctc 10457bp) and manufacturing method of xylitol thereby: Provided are a novel Candida tropicalis CJ-FID (KCTC 10457BP) derived from honey and a method for producing xylitol using the same. The method includes culturing the Candida tropicalis CJ-FID itself or an alginate-immobilized Candida tropicalis CJ-FID in a fermentation medium containing high concentration xylose and sucrose to obtain xylitol-containing culture,...

20060110812 - Methods and compositions for simultaneous saccharification and fermentation: The invention provides compositions and methods for the synergistic degradation of oligosaccharides by endoglucanases. The invention further provides recombinant host cells containing one or more genes encoding endoglucanses which are capable of the synergistic degradation of oligosaccharides. Preferred host cells of the invention are ethanologenic and capable of carrying out...

20060110813 - Process for producing l-glutamic acid: L-glutamic acid is produced by culturing in a liquid medium containing L-glutamic acid at a saturation concentration and the carbon source a microorganism that can metabolize a carbon source at a specific pH, and wherein said microorganism has an ability to accumulate L-glutamic acid in said medium in an amount...

20060110814 - Structural-based inhibitors of the glutathione binding site in aldose reductase, methods of screening therefor and methods of use: Provided herein is a crystallized ternary structure of aldose reductase (AR) bound to NADPH and γ-glutamyl-S-(1,2-dicarboxyethyl)cysteinylglycine (DCEG). Also provided are specific inhibitors of glutathione-aldehyde binding to aldose reductase which are designed via at least computer modeling of the ternary AR:NADPH:DCEG structure and methods of designing and of screening the inhibitors...

20060110815 - Personal care composition containing ozone-stressed yeast lysates: The present invention relates to an ozone-stressed yeast lysate, and a personal care composition including an ozone-stressed yeast lysate and at least one preservative selected from the group consisting of alcohols, glycols, parabens, hydantoins, quaternary nitrogen-containing compounds, isothiazolinones, aldehyde-releasing agents, and halogenated compounds. The present invention also relates to a...

20060110816 - Method of concentrating and removing harmful substance using double-stranded dna and adsorbent and apparatus therefor: There is provided a method of removing harmful substance characterized by comprising contacting a harmful substance-containing liquid to be treated with a double-stranded DNA, adsorbing the harmful substance onto the double-stranded DNA through intercalation, and separating it. The method is particularly useful for removing dioxins, and can be carried out...

20060110817 - Capturing apparatus and method of minute objects: A capturing apparatus that captures plural minute objects that float on a fluid, includes a capturing part having plural attraction ports that can attract each object, and an induction part that creates a flow in the fluid used to induce the plural objects to the attraction ports....

20060110818 - Aerosol into liquid collector for depositing particles from a large volume of gas into a small volume of liquid: An aerosol-into-liquid collector (ALC) for collecting gas-borne particles from a large volume of gas such as air into a small volume of liquid is described. The ALC uses a linear quadrupole to concentrate particles flowing in a gas and to help direct these concentrated particles toward a small volume of...

20060110819 - Apparatus and method for expression and capture of biomolecules and complexes on adsorbent surfaces: The present invention relates to the fields of molecular biology, combinatorial chemistry and biochemistry. Particularly, the present invention describes apparatus and methods for the detection and isolation of binding partners and activity modulators for biomolecules. The apparatus described allows for expression, capture and analysis of one or more biomolecules in...

20060110820 - Capturing apparatus and method of minute objects: A capturing apparatus that captures plural minute objects that float on a fluid, includes a capturing part that includes plural attraction ports, each of which can attract each object, wherein attractions by the plural attraction ports are independent of each other....

20060110821 - Devices for motile sperm separation: Device (10, 110) for separating motile sperm from a sample (100, 200), comprises a vessel (15, 115) having an inlet port (20, 120), an outlet port (35, 135) which is initially closed, a medium (40, 140) into which motile sperm in the sample (100, 200) can migrate into the vessel...

20060110822 - Perfusion bioreactors for culturing cells: A bioreactor system comprising a multi-well platform including an array of bioreactor units. The bioreactor system comprises a perfusion unit and a fluid source unit fluidly interconnected by a pumping unit. The perfusion unit comprises a multi-well plate including a plurality of main chambers configured to house or contain a...

20060110823 - Transgenic mice containing glucocorticoid-induced receptor gene disruptions: The present invention relates to transgenic animals, as well as compositions and methods relating to the characterization of gene function. Specifically, the present invention provides transgenic mice comprising mutations in a glucocorticoid-induced receptor gene. Such transgenic mice are useful as models for disease and for identifying agents that modulate gene...

20060110824 - Method for culturing avian primordial germ cells (pgcs) for a long period and preparing a medium for culturing avian pgcs for a long period: The present invention is a method for culturing avian PGCs comprising providing fibroblast cells, culturing fibroblast cells in a basal medium, collecting a conditioned medium, providing avain gonadal cells with PGCs from sex glands, and growing the PGCs in the conditioned medium supplemented with growth factors, wherein the conditioned medium...

20060110825 - Process for the preparation of stem cells from human muscle tissue and adipose tissue, and stem cell obtainable by this process: The invention relates to a process for the preparation of human stem cells from muscle tissue or adipose tissue. The process provides for the incubation of cells obtained from a sample of muscle tissue or adipose tissue in a medium comprising BSA, bFGF, EGF, VEGF, LIF, heparin and usual inorganic...

20060110826 - Methods for perfusion and plating of primary hepatocytes and a medium therefore: The present invention provides methods for culturing primary hepatocytes with improved long term function and improved viability, by plating the hepatocytes in the presence of an anti-oxidants) as well as an agent(s) which is a functional inhibitor of enzymes that generate reactive oxygen and reactive nitrogen species. One preferred embodiment...

20060110827 - Red and near infrared flourescent phyotochrome: This invention provides novel fluorescent adducts that emit in the far red and/or near infrared. In certain embodiments, the adducts comprise a mutant apoprotein and a bilin....

20060110828 - Compositions and methods for selection of a pure population of cells from a mixed population: Disclosed are methods and compositions for deriving a pure population of differentiated cells from a stem cell. The method comprises transforming a stem cell with a gene construct containing a selectable marker under the control of a tissue- or cell-type-specific regulatory element, such that the gene construct integrates at a...

20060110829 - Apparatus and system having dry gene silencing pools: A reverse transfection apparatus can be used for introducing siRNA into a cell to effect gene silencing. Such an apparatus can include a well plate having a well configured for transfecting cells. The well can include a substantially dry gene silencing composition that has at least two siRNAs which silences...

20060110830 - De-differentiation and re-differentiation of somatic cells and production of cells for cell therapies: The invention provides a method for effecting the de-differentiation of a somatic cell by culturing the cell in the absence of growth factors, cytokines, or other differentiation-inducing agents, and introducing components of cytoplasm of plutipotent cells into the somatic cell and allowing the cell to de-differentiate. The method can be...

20060105317 - Method and solutions for cryopreserving oocytes, especially fresh human oocytes: In a method for cryopreserving fresh human oocytes, freezing and thawing solutions are used, which solutions include 1,2 propanediol (PROH) and sucrose at a concentration of at least 0.3M. The oocytes are exposed for 13-15 minutes to a solution including 1.5M PROH and 0.3M sucrose before starting the freezing process....

20060105318 - Compositions and methods for non-targeted activation of endogenous genes: The present invention is directed generally to activating gene expression or causing over-expression of a gene by recombination methods in situ. The invention also is directed generally to methods for expressing an endogenous gene in a cell at levels higher than those normally found in the cell. In one embodiment...

20060105320 - Electrical conductors and devices from prion-like proteins: The present invention provides novel polypeptides comprising a prion-aggregation domain and a second domain; novel polynucleotides encoding such polypeptides; host cells transformed or transfected with such polynucleotides; novel fibrils with specific functionalities and unusually high chemical and thermal stability; and methods of making and using the foregoing....

20060105322 - Intracellular metabolic flux analysis method using substrate labeled with isotope: A method for analyzing an intracellular metabolic flux comprising determining the intracellular metabolic flux from analytical values of cells cultured in a medium containing an isotope-labeled substrate as a carbon source on the basis of an intracellular metabolic flux model constructed for the intracellular metabolic flux to be analyzed, wherein...

20060105319 - Method for converting venous blood values to arterial blood values, system for utilising said method and devices for such system: The invention relates to methods of converting venous blood values to arterial blood values. This has the advantage that arterial blood samples need not be taken, and the disadvantages compared to venous blood samples when taking arterial blood samples are eliminated. The method is basically based on three steps, namely...

20060105321 - Methods for evaluating cell membrane properties: Methods for measuring cell membrane property are disclosed. A computer readable medium includes instructions for deriving from transcellular impedance a measurement of a cell membrane property and provides the stability and error of the measurement. The derivation from the transcellular impedance includes the real and imaginary components of the transcellular...

20060105325 - Adsorbent with enhanced protein binding capacity and selectivity: The invention discloses an adsorbent composition in which each ligand has a cluster of functional groups. This adsorbent composition is designed to enhace binding affinity and specificity for biomolecules. The invention also discloses a method for adsorbing a biomolecule onto the disclosed adsorbent composition. A biomolecule can be adsorbed to...

20060105327 - Inhibition of the src kinase family pathway as a method of treating hbv infection and hepatocellular carcinoma: The present invention relates to therapeutic protocols and pharmaceutical compositions designed to target HBx mediated activation of Src kinase, members of the Src tyrosine kinase family and components of the Src kinase family signal transduction pathways for the treatment of HBV infection and related disorders and diseases, such as HCC....

20060105326 - Method for isolating proteins or protein and nucleic acid associations, or particle and protein complexes, reagent and uses: The magnetic colloidal particles comprise a core and an envelope in which the core is magnetic and is coated with at least one polymer comprising functional groups X chosen from amine, hydroxyl, thiol, aldehyde, ester, anhydride, acid chloride, carbonate, carbamate, isocyanate and isothiocyanate groups, or mixtures thereof, at least one...

20060105324 - Methods and compositions for predicting the outcome of cervical intra-epithelial neoplasia: The present invention relates to methods and kits for predicting the outcome of cervical intra-epithelial neoplasia and methods for treating a cervical intra-epithelial neoplasia. More particularly, the methods comprise the determination of the presence of the HLA-DRB1*13 allele and the absence of a high risk human papillomavirus (HPV)....

20060105323 - Multi-reporter gene model for toxicological screening: A system for the detection of gene activation events is provided which comprises a nucleic acid construct encoding a protein of the lipocalin protein family and a peptide tag in which the expression of the construct in a cell or in the cells of a transgenic animal demonstrates the activation...

20060105386 - 17867, a novel human aminopeptidase: The present invention relates to a newly identified human aminopeptidase. The invention also relates to polynucleotides encoding the aminopeptidase. The invention further relates to methods using the aminopeptidase polypeptides and polynucleotides as a target for diagnosis and treatment in aminopeptidase-related disorders. The invention further relates to drug-screening methods using the...

20060105364 - Best's macular dystrophy gene: Novel human and mouse DNA sequences that encode the gene CG1CE, which, when mutated, is responsible for Best's macular dystrophy, are provided. Provided are genomic CG1CE DNA as well as cDNA that encodes the CG1CE protein. Also provided is CG1CE protein encoded by the novel DNA sequences. Methods of expressing...

20060105383 - Biopolymer detecting method and biochip: The present invention relates to biopolymer detection utilizing antigen-antibody reaction, intended to improve the S/N ratio, to increase the detection sensitivity, and to shorten the detection time. According to the present invention, target biopolymers labeled with a fluorescent material and beads, onto the surface of which probe biopolymers and beads-ID...

20060105375 - Bioretrosynthetic methods for preparing products and compositions related thereto: Disclosed herein are methods of producing molecules in a natural system and methods for identifying metabolic pathways. Also, disclosed herein are methods and compositions for constructing biosynthetic pathways in whole cells and cell-extracts to produce molecules of interest....

20060105373 - Charge perturbation detection system for dna and other molecules: Methods and apparatus for direct detection of chemical reactions are provided. In a preferred embodiment, electric charge perturbations of the local environment during enzyme-catalyzed reactions are sensed by an electrode system with an immobilized target molecule. The target molecule is preferably DNA. The charge perturbation caused by the polymerase reaction...

20060105365 - Chimeric gb virus b (gbv-b): The present invention relates generally to the fields of biochemistry, molecular biology, and virology. More particularly, it relates to the production and use of GB virus B (GBV-B)/HCV chimeras. The invention involves nucleic acid constructs and compositions encoding GBV-B/HCV chimera. The chimeric viruses may be employed to study GBV-B and...

20060105335 - Color coated layer-by-layer microcapsules serving as combinatory analysis libraries and as specific optical sensors: Monodisperse colloids were coated with polyelectrolytes using the layer-by-layer method. The template cores can remain in the interior or be dissolved away. Various fluorescent dyes are covalently bonded, in defined quantity, to the polyelectrolytes. The quantity of dye is controlled by varying the label content or by coprecipitating unlabeled polymers....

20060105372 - Compositions and methods for purifying nucleic acids from stabilization reagents: The invention features reagents, methods and kits for the purification of RNA, or DNA, or both, from a sample....

20060105348 - Compositions and methods for the detection and discrimination of nucleic acids: The present invention provides novel primers and methods for the detection of specific nucleic acid sequences. The primers and methods of the invention are useful in a wide variety of molecular biology applications and are particularly useful in allele specific PCR....

20060105362 - Compositions and systems for identifying and comparing expressed genes (mrnas) in eukaryotic organisms: The invention comprises compositions and systems to identify and compare expressed genes in a given in vivo or in vitro RNA sample, as well as the relative difference in mRNA expression between two or more samples, where desired. Furthermore, the invention comprises compositions and systems to identify novel genes. The...

20060105342 - Computerized systems for formation and update of databases: An apparatus for creating and maintaining a database containing information relating to residential properties includes an AVM device configured to receive information relating to residential properties, producing AVM values for each property and updating an AVM database with the AVM values....

20060105384 - Detection of dna damage: The health condition of a living organism is detected by electrochemically analyzing samples from selected areas of the body of said living organism for elevated free levels of nucleotide excision products resulting from DNA or RNA damage....

20060105349 - Device and method for purification of biological materials: An apparatus, method and kit for isolating a biomolecule from a sample. The sample comprises a complex biological material, which includes insoluble matter. Some embodiments of the apparatus and kit include a reservoir and means for capturing the biomolecule either contained within or coupled to the reservoir. The reservoir can...

20060105360 - Diagnosis and treatment of cancers with microrna located in or near cancer associated chromosomal features: MicroRNA genes are highly associated with chromosomal features involved in the etiology of different cancers. The perturbations in the genomic structure or chromosomal architecture of a cell caused by these cancer-associated chromosomal features can affect the expression of the miR gene(s) located in close proximity to that chromosomal feature. Evaluation...

20060105330 - Diagnostics and remedies for malignant brain tumor: It is intended to provide a diagnosis method and diagnostics for cancers such as human glioma and a therapeutic method and remedies for cancers such as human glioma by identifying tumor suppressive genes or cancer genes useful in diagnosing or treating cancers such as human glioma. Tumor suppressive genes such...

20060105361 - Donor yeast strain for transfer of genetic material: The invention provides a universal yeast donor strain that contains a conditional centromere and a URA3 allele on every chromosome. This strain was constructed in four rounds of crosses of individual conditional chromosome strains using a novel tetrad-based screen to identify segregants in which all marked chromosomes were contained in...

20060105355 - Electrode array device having an adsorbed porous reaction layer having a linker moiety: There is disclosed an electrode array device having an adsorbed porous reaction layer having a linker attacher thereto for improved synthesis quality. The array comprises a plurality of electrodes on a substrate, wherein the electrodes are electronically connected to a computer control system. The array has an adsorbed porous reaction...

20060105329 - Gene panel participating in liver regeneration: A gene panel comprising a group of genes of which expression levels change in hepatocytes during liver regeneration as compared with those in a normal state is produced by the following steps: (a) the step of measuring expression levels of various genes in hepatocytes of a model animal in a...

20060105346 - Human prostaglandin fp receptor variants and methods of using same: The invention provides an isolated polypeptide containing the amino acid sequence of SEQ ID NO: 17, 18, 19, 20, 21, 22, 23, 24, or 25. The invention also provides an isolated polypeptide containing an amino acid sequence having at least 50% amino acid identity with SEQ ID NO: 14 and...

20060105336 - Isolation and identification of t cells: The present invention relates to improved autologous T cell vaccines and improved methods for their production. The invention is also directed to methods for treating autoimmune diseases such as multiple sclerosis or rheumatoid arthritis using autologous T cell vaccines. The invention is further directed to the diagnosis of T cell...

20060105328 - Mab-based dot-elisa method and assay kit for the detection of viruses: A monoclonal antibody-based Dot-ELISA assay for the rapid detection of animal viruses such as avian influenza virus. The assay includes applying a specimen suspected of containing an animal virus on a porous membrane and treating the specimen with a solution of citric acid or lactic acid and a solution containing...

20060105359 - Method and apparatus for automated pre-treatment and processing of biological samples: The present invention concerns a method and apparatus for automatic processing a biological sample on a carrier, perhaps robotically, by applying predetermined amounts of reagents in a predetermined sequence according to a processing protocol, the processing including pre-treatment steps, under the control of an adaptive processing control system using a...

20060105338 - Method and apparatus for correcting position and attitude of object to be held: There is provided a method of correcting position and attitude of an object to be held capable of reducing the number of image processing steps and simplifying the method of correcting shifts in the position and attitude of the object with respect to a holding member. The object to be...

20060105371 - Method and device for protein delivery into cells: Methods for performing surface-mediated protein delivery into living cells, and fabricating protein-transfected cell cluster arrays are provided. The method comprises providing a protein-containing mixture; depositing said protein-containing mixture onto a surface at defined locations; affixing the protein-containing mixture to the surface as microspots; and plating cells onto the surface in...

20060105368 - Method and device for simultaneously molecularly cloning and polylocus profiling of genomes or genome mixtures: A method for amplifying genetic material to characterize complex populations of nucleic acids and a method for applying these characterizations to applications. The characteristics of a population of nucleic acids may be an index of disease states as for example when pathogens cause nucleic acid release into body fluids or...

20060105367 - Method and markers for the diagnosis of graft versus host disease (gvhd): A method for the diagnosis of GvHD, the method comprising: c) measuring the presence or the absence of a polypeptide marker in a urine sample, wherein the polypeptide marker is selected from the group of polypeptide markers shown in table 1, and d) comparing the probability of the presence of...

20060105351 - Method and system for nucleic acid detection: The present invention relates to a method of detecting nucleic acids comprising suspending at least one recognizable target nucleic acid in a suspending solution containing polymeric particles marked with a probe, wherein the probe has an affinity for the target nucleic acid, adding a recognition unit-peroxidase conjugate marker to the...

20060105350 - Method and system for sorting and separating particles: The present invention relates to a method of particle separation comprising preparing a suspension of particles containing at least one recognizable target particle in a suspending solution, labeling the target particle with a conjugate marker, wherein the conjugate marker comprises at least one recognition unit for the recognizable target particle...

20060105345 - Method for diagnosing lung transplantation rejection: The invention relates to a method for diagnosing lung transplantation rejection comprising determining the amount of Hepatocyte Growth Factor (HGF) in a body fluid or tissue sample of a patient who has undergone lung transplantation....

20060105333 - Method for diagnosis of intestinal-type gastric tumors: Objective methods for detecting and diagnosing intestinal-type gastric cancers are described herein. Also described is method for predicting the presence or absence of lymph node metastasis (i.e., identifying the metastatic phenotype). In one embodiment, the diagnostic method involves the scoring of gene expression profiles that discriminate between lymph node positive...

20060105352 - Method for sensitive detection of multiple biological analytes: The present invention relates to a method of detecting biological analytes comprising suspending a target analyte in a suspending solution containing polymeric particles marked with a probe, wherein the probe has an affinity for said target analyte; adding recognition unit-peroxidase conjugate marker to the suspending solution; forming a complex of...

20060105347 - Method for the production of fusion proteins in transgenic mammal milk: Desirable fusion proteins can be produced in and purified from the milk of transgenic animals. The peptides are made as fusion proteins with a suitable fusion partner such as human alpha-fetoprotein. The fusion partner protein acts to promote and increase the half-life of the overall molecule as well as having...

20060105334 - Method of determining the copy number of a nucleotide sequence: The invention relates to a method of determining of accurately determining the copy number of a nucleotide sequence I in a sample using an amplification technique, such as PCR. In addition, a second nucleotide sequence II is also measured and calibration curves for each are made, from which the relative...

20060105370 - Method of nucleic acid typing for selecting registered donors for cross-matching to transfusion recipients: Disclosed are a method and an algorithm for genetic cross-matching based on the comparison of recipient and donor genotypes—and the underlying combinations of alleles and haplotypes. The method of the invention, rather than focusing on phenotype prediction, instead relies on a comparison of genetic variants identified in the recipient and...

20060105369 - Methods and kits for identifying enterohaemorrhagic escherichia coli o157:h7 strain: Disclosed is a method for identifying the presence of Enterohaemorrhagic E. coli O157:H7 strain using primers or a probe prepared in accordance with SEQ ID NO: 1 or a sequence homologous thereto. A kit for identifying Enterohaemorrhagic E. coli O157:H7 strain is also disclosed....

20060105380 - Methods and systems for dynamic gene expression profiling: The invention provides compositions, methods and systems for dynamic transcription profiling of two or more samples. The method of the invention comprises the uses of sample-specific primers for cDNA synthesis and for subsequent amplification of the synthesized cDNAs. The levels of abundance of genes are compared between samples for the...

20060105363 - Methods for determining transcriptional activity: In some embodiments of the invention, methods are provided to interrogate the transcriptional activity. The methods employ hybridization of a large number of oligonucleotide probes with nucleic acid derived from RNAs in a cellular compartment....

20060105343 - Methods for diagnosis and prognosis of cancer: We have discovered a protein in humans, herein referred to as thymosin R16 (SEQ ID NO: 1), that is expressed in human prostate cancer tumors but not in specimens of benign prostate hyperplasma (BPH) tissues. In contrast, prostate specific antigen (PSA), the gold standard of prostate cancer diagnosis, is highly...

20060105331 - Methods for improving rna transcription reactions: Methods are described for eliminating single-stranded oligonucleotides from a sample prior to RNA transcription, thereby reducing non-template derived production of RNA. In one embodiment, a sample containing the template for RNA transcription is treated with one or more exonucleases to remove single-stranded oligonucleotides from the reaction mixture prior to RNA...

20060105339 - Methods for measuring the rates of replication and death of microbial infectious agents in an infected: The present invention provides methods and kits useful for determining rates of replication and destruction of an infectious agent within an infected host organism. In the methods of the invention, an isotopically-labeled precursor molecule is administered to an infected host, and is given sufficient time to pass into the host's...

20060105356 - Methods for reducing evaporation in wet processing steps: Methods of contacting a surface of an object with a liquid are provided. Aspects of the invention include positioning an object in a reaction chamber and flowing a liquid across a surface of the object, where at least a portion of the flowing step occurs in the presence of a...

20060105382 - Microfluidic channels with attached biomolecules: An exemplary system and method for bonding substrate layers in the presence of chemically active species to form functionalized microfluidic surfaces is disclosed as comprising inter alia a first substrate (100), a second substrate (200), a chemically functional species (120) attached to first substrate (100), and a radiatively absorptive mask...

20060105344 - Modulation of deubiquitinase family members: Modulation of Deubiquitinase Family Members The present invention relates to the therapeutic treatment of conditions by modulating members of the deubiquitinase family, and also to assays for identifying substances which may be useful in such treatments, as well as to the treatment of cylindromatosis and more generally to the modulation...

20060105353 - Non-invasive prenatal fetal cell diagnostic method: The present invention provides methods for prenatal diagnosis. The method includes distinguishing fetal cells from maternal cells. The fetal cells may be analyzed to measure, for instance, the presence or absence of genetic markers....

20060105376 - Novel full-length cdna: Novel full-length cDNAs are provided. 2443 cDNA derived from human have been isolated. The full-length nucleotide sequences of the cDNA and amino acid sequences encoded by the nucleotide sequences have been determined. Because the cDNA of the present invention are full-length and contain the translation start site, they provide information...

20060105340 - Novel tumor suppressor gene and compositions and methods for making and using the same: The present invention relates to the identification and cloning of ARTS-1, a novel tumor suppressor gene, to isolated proteins encoded by ARTS-1, and to methods of making and using the same....

20060105358 - Nucleic acid cloning: The present invention provides an improved system for linking nucleic acids to one another. In particular, the present invention provides techniques for producing DNA product molecules that may be easily and directly ligated to recipient molecules. The product molecules need not be cleaved with restriction enzymes in order to undergo...

20060105381 - Polymorphisms of the octn1 cation transporters associated with inflammatory bowel disorders: The invention provides a method for diagnosing Inflammatory Bowel Diseases, using genetic markers that are implicated in severe, early-onset Crohn's Disease (CD). The invention also provides coding sequence mutations in the OCTN1 gene that significantly reduces its ability to transport the organic cation carnitine. The invention further provides mutations in...

20060105379 - Polynucleotide encoding a novel metalloprotease highly expressed in the testis, mmp-29: The present invention provides novel polynucleotides encoding MMP-29 polypeptides, fragments and homologues thereof. Also provided are vectors, host cells, antibodies, and recombinant and synthetic methods for producing said polypeptides. The invention further relates to diagnostic and therapeutic methods for applying these novel MMP-29 polypeptides to the diagnosis, treatment, and/or prevention...

20060105354 - Real-time quantification of multiple targets on a micro-array: The present invention relates to a method and apparatus for monitoring a real-time quantification of multiple target molecules during their binding on capture molecules of a micro-array. The method comprises the steps of: placing, in a chamber (14), a support (15) having fixed upon its surface a micro-array comprising at...

20060105374 - Rhob variants and methods of use: In one aspect, the present invention concerns RhoB variant polypeptides and isolated degenerate polynucleotides encoding the RhoB variant polypeptides. In another aspect, the present invention concerns nucleic acid constructs containing a polynucleotide encoding a RhoB variant polypeptide, and host cells genetically modified to express such polynucleotides. In another aspect, the...

20060105377 - Screening to optimize rnai: Methods and compositions are provided for screening RNAi molecules for efficiency of modulation, particularly inhibition, of expression of genes. The gene for the target protein is fused to a DNA sequence encoding a small fragment of β-galactosidase. The fragment is competent to complex with a large fragment of β-galactosidase to...

20060105337 - Solid phase based nucleic acid assays combining high affinity and high specificity: The invention relates to methods for detection of nucleic acids on a solid phase, combining high affinity and high specificity. More particularly, the invention relates to methods combining high-affinity hybridization with highly specific enzymatic discrimination in solid phase based nucleic acid assays. This invention further relates to kits containing the...

20060105366 - Specific base sequence detection method and primer extension reaction detection method: A specific base sequence detection method, comprising preparing a sample solution including a target nucleic acid, a primer for amplifying a specific base sequence and whose end has a site to be coupled to an electrode, and nucleotide; extending the primer if the specific base sequence is present in the...

20060105378 - Tenascin-c nucleic acid ligands: Methods are described for the identification and preparation of nucleic acid ligands to tenascin-C. Included in the invention are specific RNA ligands to tenascin-C identified by the SELEX method. Further included in the invention are methods for detecting the presence of a disease condition in a biological tissue in which...

20060105357 - Tissue sensor and uses thereof: Described are assemblies for screening a compound for bioactivity, the assemblies comprising a tissue and a sensor. A change in a biological parameter is measured by the sensor, such that a change in a parameter occurring when the tissue is contacted with a candidate compound is detected by the sensor....

20060105341 - Trap-tagging: a novel method for the identification and purification of rna-protein complexes: Conventional methods for the isolation and identification of specific RNA-protein complexes are plagued by a number of problems not encountered in genomics or proteomics. Here we describe a two step affinity purification method used to isolate RNA-protein complexes. The TRAP (Tandem RNA Affinity Purification) tag is a dual RNA tagging...

20060105332 - Treatment of tissue specimens: Apparatus for treating tissue specimens comprises a stationary outer casing (2) in the top of which is positioned an annular trough (3) for holding a liquid. Rotatably mounted in the outer casing (2) is a central hub (5) supporting a disc-like lid (6) the underside of which carries a magnet...

20060105385 - Xenobiotic related induction of gene expression: An isolated nucleic acid molecule comprising a nucleotide sequence encoding a transcriptional enhancer of cytochrome P450 (P450) CYP3A4 production or expression, and uses of the nucleic acid molecule for screening compounds for xenobiotic induction of CYP3A4 expression in cells and animals....

20060105391 - Device and method for separating molecules: An apparatus and method for separating a biomolecule from one or more contaminants in a sample. The apparatus can include a unitary device comprising a plurality of fractionation devices, each reservoir comprising a filter, each reservoir adapted to contain a solid phase, the solid phase adapted to separate the biomolecule...

20060105392 - Differential diagnosis of vitamin b12, vitamin b6, and folic acid disorders: The invention concerns a method for determining vitamin B12, B6 or/and folic acid disorders and in particular the differential diagnosis of vitamin B12, vitamin B6 or/and folic acid disorders by means of three or four independent parameters. The differential diagnosis can be used to detect a vitamin B12, vitamin B6...

20060105388 - Incorporation of hapten groups during the production of carriers for the determination of analytes: The invention relates to a method for producing a carrier, especially microfluidic carrier, for the determination of analytes....

20060105393 - Ligand-receptor tracking assays: The present invention provides an image-based method of screening for compounds based on the measurement of internalization of a fluorescently labeled ligand. It further provides a multiplex method for high content screening of compounds wherein at least two cellular parameters are measured....

20060105389 - Method for obtaining single chain antibodies to human interferon alpha2b: A bacterial high-expression system which is applicable for simultaneous screening of large numbers of recombinant clones from combinatorial antibody libraries is disclosed. The method pertains to screening of single chain antibodies from libraries expressed in the periplasm of E. coli by secretion. By this approach, approximately 104 clones can be...

20060105397 - Method for the detection of stress biomarkers including cortisol by fluorescence polarization: The inventive subject matter relates to a competitive method measuring stress biomarkers in bodily fluids including serum, urine and oral fluids including saliva. The inventive method measures biomarkers including cortisol, melatonin and secretory IgA by fluorescence polarization fluorescence lifetime analysis or fluorescence resonance energy transfer....

20060105390 - Method of characterizing heterogeneity in receptor-ligand interactions and related method of assay signal histogram transformation: Disclosed is a method for increasing the degree of confidence in receptor-ligand and probe-target interaction assays involving ligand or target capture by a heterogeneous population of receptors or probes displayed on solid phase carriers. The method comprises a process of establishing a weight function reflecting the heterogeneity in the receptor...

20060105394 - Methods and compositions for treatment and prevention of major depressive disorder: The present invention relates to methods of diagnosing, prognosing or treating diseases or disorders in which elevated levels of Abeta protein, including abeta1-42 are prevalent. In particular, the present invention relates to methods of diagnosing, prognosing or treating a major or minor depressive episode/disorder attributed to elevated levels of Abeta...

20060105395 - Methods and systems for positioning microspheres for imaging: Various methods and systems for positioning microspheres for imaging are provided. One system includes a filter medium that includes openings. The openings are spaced in a substantially equidistant manner across the filter medium. The system also includes a flow subsystem coupled to the filter medium. The flow subsystem is configured...

20060105396 - Novel epithelial tissue imaging agent: Targeting molecules are provided for use in delivering imaging agents to epithelial tissue. The targeting molecule comprises a polypeptide that forms a closed covalent loop, contains at least three peptide domains having β-sheet character, each of the domains being separated by domains lacking β-sheet character. The targeting molecule specifically binds...

20060105387 - Transferrin fusion proteins libraries: Modified fusion proteins of transferrin and therapeutic proteins or peptides with increased serum half-life or serum stability are disclosed. Preferred fusion proteins include those modified so that the transferrin moiety exhibits no or reduced glycosylation, binding to iron and/or binding to the transferrin receptor....

20060105399 - Materials and procedures for the purification of cells: The subject invention provides new materials and methods for the efficient isolation and purification of stem cells. Specifically, conductive immunopolymers with stem cell specific antibodies can be used to remove stem cells from biological fluids....

20060105398 - Methods of identifying compounds that modulate igg mediated mast cell activation: The present invention relates to methods for identifying compounds capable of modulating Fcg receptor signaling pathway in mast and/or basophil cells. The invention further relates to methods for identifying compounds for use as therapeutic agents in the treatment of disorders related to activation of Fcg receptor signaling cascade....

20060105402 - Blood type method system and device: A method for the detection and/or visualization of particle agglutination in a particle suspension is provided, comprising placing a volume of the particle suspension and a volume of a solution or suspension containing an agglutinating agent at substantially the same selected location on a surface of a filter constructed so...

20060105403 - Braca1/acc alpha molecular complexes, diagnostic and therapeutic applications: The invention concerns a molecular complex comprising: a first polypeptide including the sequence of amino acids 1640 to 1863 the human BRCA1 protein or a similar sequence of amino acids of the protein BRCA1 in another animal species, and a second polypeptide including a fragment of the ACC-α protein capable...

20060105404 - Fluorescence polarization assay for determining histidine decarboxylase activity: A fluorescence polarization assay for determining the HDC modulating activity of a candidate compound comprising the steps of: a) providing a reaction mixture comprising a HDC, histidine, a fluorescently labeled histamine probe, a candidate compound and an anti histamine antibody having selectivity for histamine at least 10 fold greater than...

20060105405 - Migration inhibitory factor in serum as a tumor marker for prostate, bladder, breast, ovarian, kidney, pancreatic and lung cancer and for diagnosis of endometriosis: Macrophage migration inhibitory factor (MIF) has been found to be overexpressed in blood of individuals having various cancers, including prostate, PIN, lung, breast, ovary, kidney, pancreatic and bladder, as well as inflammatory diseases and endometriosis. Normal levels of MIF in serum of males and females without these cancers have been...

20060105406 - Cytoplasmic antigens for detection of candida: The present invention relates to a method and a means of diagnosing Candida infection. In particular the present invention relates to a method of diagnosing Candida infection by measuring the levels of antibody to Candida cytoplasmic antigen present in a biological sample taken from a subject at risk of, or...

20060105401 - Diagnostic method for screening complement regulatory protein levels to predict spontaneous abortion: The invention provides a method for the early detection of pregnancy failure, spontaneous abortion or premature birth by determinations of complement regulatory protein levels. A kit for use in rapid identification of these pregnancy complications is also provided....

20060105400 - Elisa kits for detecting collagenase 3 as a proenzyme and in an activated form in body fluids and cell culture supernatants: The invention relates to ELISA kits for detecting pro-collagenase 3 and activated collagenase 3 in body fluids, especially in human serum and synovial fluid, and in cell culture supernatants, and monoclonal antibodies which specifically recognise said antigens. Said ELISA kit comprises the following separately packed elements: a) a sol