Chemistry: molecular biology and microbiology inventions 03/06

20060068369 - Blood component separation method and apparatus: An apparatus and method for collecting whole blood and then separating it into components for subsequent use or storage. A self-contained bag set is used to collect the sample, which may then be placed into a device adapted to fit into a centrifuge for separation of components. Each component is...

20060068374 - Cell motility assay: An electrooptical method is disclosed for quantifying the motility response of cells to external stimuli, wherein a target cell population (formed as a function of said response) is subsequently optically differentiated by the addition of an optical differentiation solution, and whereby the targeted cell population becomes more or less detectable...

20060068370 - Liver specific chimeric regulatory sequence and use thereof: The present invention relates to a chimeric regulatory sequence with liver cell specificity. The chimeric regulatory sequence includes a proximal regulatory sequence and a distal enhancer of 5′ flanking region of human α-fetoprotein (AFP) gene. The chimeric regulatory sequence is useful in purified specific cell lineages, such as liver cells,...

20060068375 - Method and apparatus for computer controlled rare cell, including fetal cell, based diagnosis: A computer controlled method for detecting and diagnosing a rare cell type in a tissue sample is provided, said method comprising treating the tissue sample such that it generates a first signal indicative of the presence at a location of a rare cell, detecting the first signal, treating the location...

20060068376 - Method and apparatus for computer controlled rare cell, including fetal cell, based diagnosis: A computer controlled method for detecting and diagnosing a rare cell type in a tissue sample is provided, said method comprising treating the tissue sample such that it generates a first signal indicative of the presence at a location of a rare cell, detecting the first signal, treating the location...

20060068377 - Method and apparatus for computer controlled rare cell, including fetal cell, based diagnosis: A computer controlled method for detecting and diagnosing a rare cell type in a tissue sample is provided, said method comprising treating the tissue sample such that it generates a first signal indicative of the presence at a location of a rare cell, detecting the first signal, treating the location...

20060068372 - Method of classifying plant embryos using penalized logistic regression: A method is disclosed for classifying plant embryos according to their quality using a penalized logistic regression (PLR) model. First, sets of image or spectral data are acquired from plant embryos of known quality, respectively. Second, each of the acquired sets of image or spectral data is associated with one...

20060068371 - Methods for analyzing inter-cellular phenomena: Aspects of the present invention encompass the collection of multispectral images from a population of objects, and the analysis of the collected images to measure at least characteristic of the population, using photometric and/or morphometric features identifiable in the collection of images. In an exemplary application, the objects are biological...

20060068373 - Systems and methods of identifying biomarkers for subsequent screening and monitoring of diseases: A system for generating an image of ultrastructural biomarkers from a biological sample is provided. The system includes a grid onto which a sample to be imaged may be placed and a cryogenic reservoir into which the grid and sample may be immersed for vitrification of the sample. The system...

20060068380 - Assay for detecting and quantifying hiv-1: Compositions, methods and kits for detecting HIV-1 nucleic acids using nucleic acid amplification. Particularly described are oligonucleotides that are useful as hybridization probes and amplification primers for detecting very low levels of HIV-1 nucleic acids by real-time monitoring of amplicon production. The invented assays are characterized by high levels of...

20060068381 - Methods for identifying a peptide that binds a geometrical shape: Provided herein are methods, such as phage display assays, for bioengineering peptides that bind to geometrically and/or atomically structured molecular surfaces such as, for example, flat surfaces, smooth curved surfaces, as well as surfaces with periodic, random, or fractal atomic configurations. Surface-binding peptides are provided that are identified using the...

20060068378 - Nanoparticles having oligonucleotides attached thereto and uses therefor: The invention provides methods of detecting a nucleic acid. The methods comprise contacting the nucleic acid with one or more types of particles having oligonucleotides attached thereto. In one embodiment of the method, the oligonucleotides are attached to nanoparticles and have sequences complementary to portions of the sequence of the...

20060068379 - Phage display of intact domains at high copy number: Disclosed is a phage display system in which the molecules to be displayed (i.e., molecules of interest) are bound to dispensable capsid polypeptides such as SOC (small outer capsid) and HOC (highly antigenic outer capsid) polypeptides that are, in turn, bound to a surface lattice protein, such as those on...

20060068441 - Alternative pol kappa nucleotide and amino acid sequence and methods for using: The present invention provides isolated nucleic acid sequences and expression vectors encoding the pol κC76, substantially purified pol κ76, and methods for detecting pol κ76....

20060068432 - Biological markers for longevity and diseases and uses thereof: This invention provides methods of using of the sizes and levels of high-density lipoprotein (HDL) and low-density lipoprotein (LDL) particles, the −641 allele of the promoter of the gene encoding apolipoprotein C-3 (APOC-3), the 405 allele of the gene encoding cholesteryl ester transfer protein (CETP), and plasma levels of insulin-like...

20060068401 - Biopolymer resonant tunneling with a gate voltage source: The invention provides an apparatus and method for sequencing and identifying a biopolymer. The invention provides a first electrode, a second electrode, a first gate electrode, a second gate electrode, a gate voltage source and a potential means. The gate electrodes may be ramped by a voltage source to search...

20060068424 - Biosensor: An object of the present invention is to provide a biosensor wherein at least two types of surfaces are patterned without impairing the sensor surface and non-specific adsorption is thereby suppressed. The present invention provides a biosensor comprising a substrate coated with a hydrophobic compound having a photoactive group, or...

20060068411 - Cancer specific gene mh15: MH15 (Hn1L) is identified as an oncogene. Methods and compositions for detecting and diagnosing cancer in patients are provided, by determining the level of MH15 expression in biological samples. Also provided are methods for screening for inhibitors and moderators of MH15 expression and activity, as well as compositions comprising compounds...

20060068386 - Complete genome and protein sequence of the hyperthermophile methanopyrus kandleri av19 and monophyly of archael methanogens and methods of use thereof: We have determined the complete 1,694,969 nucleotide sequence of the GC-rich genome of Methanopyrus kandleri using a novel approach. It is based on unlinking genomic DNA with the ThermoFidelase version of M. kandleri topoisomerase V and cycle sequencing directed by 2′-modified oligonucleotides (Fimers). 3.3× sequencing redundancy was sufficient to assemble...

20060068415 - Complexity management of genomic dna by locus specific amplification: The present invention provides for novel methods and kits for reducing the complexity of a nucleic acid sample to interrogate a collection of target sequences. In one embodiment complexity reduction can be accomplished by extension of a locus specific capture probe followed by amplification of the extended capture probe using...

20060068436 - Compositions and methods for cdna synthesis: Methods for making cDNA molecules, for amplification of RNA by PCR and for preparation of cDNA libraries are provided. Kits for making cDNA molecules also are provided. Compositions are also provided comprising mixtures of reagents, including reverse transcriptases, buffers, cofactors and other components, suitable for immediate use in conversion of...

20060068421 - Compositions and methods for phage display of polypeptides: The present invention relates in part to novel polypeptide display methods that can permit the efficient display and selection of polypeptides, and to display vectors and other compositions configured for efficient use in such methods....

20060068426 - Cyclic peptides and antibodies thereof: The present invention provides methods of preparing and identifying antibodies against a loop domain of a protein, such as an extracellular loop (ECL) domain of a transmembrane protein. Cyclic and end-to-end cyclized peptides corresponding to loop domains are employed in the present invention. Transmembrane proteins contemplated by the invention include...

20060068419 - Detection of nucleic acids to assess risk for bovine spongiform encephalopathy: The present invention provides a method of detecting abnormal serum nucleic acid profiles to assess the risk of a transmissible spongiform encephalopathy, e.g., BSE....

20060068400 - Device and method for fecal testing: A specimen testing device having a first panel with at least two apertures, a second panel with at least two apertures opposite the apertures in said first panel, a sheet disposed between the first and second panels for receiving a specimen through the apertures, the sheet in the apertures in...

20060068416 - Devices coated with substances which mediate the adhesion of biological material: Devices having at least one surface which comes into contact with tissues and/or fluids of the human or animal body. These surfaces are at least partially coated with aptamers, which mediate the adhesion of biological material....

20060068390 - Dna amplification and sequencing in collapsible emulsions: The present invention relates to a method of performing a chemical reaction, in particular a small-scale chemical reaction. The method involves the use of two (or more) phases which, when formed into an emulsion, have the characteristic of being subject to “collapse” under certain physical or chemical conditions such that...

20060068422 - Gene chip technology for determining memory genes: The present invention relates to methods of identifying genes involved in memory formation. This is accomplished by performing a gene chip identification of those genes expressed during transcription-dependent memory formation but not during transcription-independent memory formation. A statistical analysis of the gene chip identification output yields a set of genes...

20060068425 - Genes, compositions, kits, and methods for identification, assessment, prevention, and therapy of prostate cancer: The invention relates to newly discovered nucleic acid molecules and proteins associated with prostate cancer including pre-malignant conditions. Compositions, kits, and methods for detecting, characterizing, preventing, and treating human prostate cancers are provided....

20060068387 - Genetic marker for endocrine disorders: A genetic marker associated with polycystic ovary syndrome is disclosed. The presence or absence of the allele is highly predictive of whether an individual is at risk from polycystic ovary syndrome. Methods of diagnosis, markers, and primers are disclosed and claimed in accordance with the present invention....

20060068397 - High throughput screening method for identifying molecules having biocidal function: Novel methods for screening and cloning DNA sequences coding for biocidal molecules, and for identifying biocidal molecules involving use of viability staining assay are provided. The methods consist of construction of libraries of DNA from natural and synthetic sources, and expression of cloned DNA in surrogate hosts, identification of DNA...

20060068414 - Identification of aging genes through large-scale analysis: High throughput methods for screening genetic variants that are phenotypically distinguishable are provided. Methods for identifying “long-lived” genetic variants among a set of variants are also provided. Methods for identifying pharmaceutical compounds that can promote longevity in various subjects, including mammals, and that can delay the onset of various diseases...

20060068438 - Identification of gene expression alterations underlying the aging process in mammals: A method of measuring the biological age of a multicellular organism is disclosed. In one embodiment, the method comprises the steps of: (a) obtaining a sample of nucleic acid isolated from the organism's organ, tissue or cell, wherein the nucleic acid is RNA or a cDNA copy of RNA and...

20060068428 - Identification of genetic markers associated with parkinson disease: The present invention provides methods and compositions for screening a subject for Parkinson disease, for increased risk of developing Parkinson disease and/or for an earlier or later age of developing Parkinson disease, comprising detecting the presence of a genetic marker associated with Parkinson disease....

20060068418 - Identification of markers in lung and breast cancer: Methods for identifying expression of markers indicative of the presence of breast cancer and lung cancer are provided. Also provided are articles of manufacture useful in such methods and compositions containing primers and probes useful in such methods....

20060068384 - Immuno polymerase chain reaction assay: A method to detect biological molecules in a sample which is an immuno polymerase chain reaction assay which comprises the detection of a complex which includes a ligand: nucleic acid conjugate bound to at least one biological molecule. The complex is detected by the addition of a second nucleic acid...

20060068412 - Integrated multistep bioprocessor and sensor: The invention provides an integrated biosensor. The integrated bioprocessor consists of an integrated capture chamber having an analyte recognition coating and a structure supporting analyte detection, analyte growth and target nucleic acid detection. The integrated capture chamber can consist of a waveguide, a capillary tube, a mixing flow chamber or...

20060068382 - Isolated human transporter proteins, nucleic acid molecules encoding human transporter proteins, and uses thereof: The present invention provides amino acid sequences of peptides that are encoded by genes within the human genome, the transporter peptides of the present invention. The present invention specifically provides isolated peptide and nucleic acid molecules, methods of identifying orthologs and paralogs of the transporter peptides, and methods of identifying...

20060068383 - Kits for the detection of target sequences: The present invention relates to means for cleaving a nucleic acid cleavage structure in a site-specific manner. Enzymes, including 5′ nucleases and 3′ exonucleases, are used to detect and identify nucleic acids derived from microorganisms. Methods are provided which allow for the detection and identification of bacterial and viral pathogens...

20060068429 - Leprotl1 and ob-rgrp genes for screening compounds that act on the gain or loss of weight or on diabetes in a human or animal subject: A method for identifying compounds acting on weight gain or weight loss or on diabetes in a human or animal subject including measuring effect of the compounds on (a) expression of at least one of genes LEPROTL1 and OB-RGRP or a part of them and/or (b) intracellular transport up to...

20060068403 - Long oligonucleotide arrays: Long oligonucleotide arrays, as well as methods for their preparation and use in hybridization assays, are provided. The subject arrays are characterized in that at least a portion of the probes of the array, and usually all of the probes of the array, are long oligonucleotides, e.g. oligonucleotides having a...

20060068437 - Meg-3 protein: The present invention provides a DNA expressed at high frequency in mesangial cells and a protein (Meg-3) encoded by this DNA. These substances are useful in, for example, identifying mesangial cells and detecting abnormalities in mesangial cells. Moreover, the above protein would be helpful for clarification of the functions of...

20060068396 - Method for biochip detecting limited cells: The present invention is a method for a biochip detecting limited cells. The present invention comprises steps of: obtaining a nylon membrane chip having required nucleotide fragments arranged in a dot matrix way by using a manual spotter; naturally drying by heat and fixing the nucleotide fragments on the nylon...

20060068408 - Method for determining dna damage and multi-chamber plate: In a method for determining DNA damage, wherein cells are treated with genotoxic agents and lysed and DNA fragments of the cells are separated according to their length by a physical separating method, cells are put into various chambers of a multi-chamber plate and a genotoxic chemicals are put into...

20060068435 - Method for screening cells and method for detecting oral carcinoma cells: The present invention provides a method of detecting oral carcinoma cells with high accuracy, and a method of making possible early detection of oral carcinoma or discrimination between a precancerous lesion and an early cancer in diagnosis of oral carcinoma. The methods are achieved by screening cells for oral carcinoma...

20060068442 - Method for the identification of extracellular domains of epstein barr virus (ebv) tumor-associated latent membrane proteins and for the selection of antibody reagents reactive therewith: Described are a method for the identification of extracellular epitopes of Epstein Barr virus (EBV) encoded membrane proteins, expressed on the outer cell surface of EBV-transformed mammalian cells, methods fo rthe selection and preparation of antibody reagents specific for the said epitopes, as well as peptides, including extracellular domains of...

20060068388 - Method for the identification of modulators of a secretase activity: The present invention relates to a cellular screening method for the identification of modulators of secretases. Said method comprises contacting suitable host cells with a test substance wherein said suitable host cells comprise: a) a fusion protein secretory protein, a membrane anchor domain and a secrease cleavage sequence, b) a...

20060068392 - Method of fixing biomolecule on metal support: A solution of a nucleic acid is spotted on a metal carrier consisting of a metal selected from Groups I, II, III, IV, V, VI, and VII of second to seventh periods and transition elements in a periodic table, or of an alloy containing the metal, and the solution is...

20060068407 - Method of immobilizing nucleic acid aptamers: The present invention relates to methods of immobilizing nucleic acid aptamers within a sol-gel matrix. The aptamer system remains functionally intact when it is immobilized within a protein and membrane-compatible sol-gel derived from polyol silane precursors or sodium silicate....

20060068394 - Method of producing a dna library using positional amplification: The disclosed invention relates to general and specific methods to use the Primer Extension/Nick Translation (PENT) reaction to create an amplifiable DNA strand, called a PENTAmer. A PENTAmers can be made for the purpose of amplifying a controlled length of DNA located at a controlled position within a DNA molecule,...

20060068434 - Methods and compositions for detecting cancer using components of the u2 spliceosomal particle: The present invention relates to cancer-associated proteins and nucleic acids that encode or bind specifically to cancer-associated proteins, which represent markers for cancer detection. Specifically, the invention provides a family of methods and compositions for detecting cancer, for example, breast cancer, in an individual using components of the U2 spliceosomal...

20060068389 - Methods and compositions for inhibiting hiv replication: Pharmaceutial composition comprising compounds and/or compositions useful to inhibit HIV replication are disclosed. Methods of treating individuals infected with HIV are disclosed. Methods of preventing HIV infection in high-risk individuals are disclosed....

20060068417 - Methods and compositions to detect nucleic acids in a biological sample: Methods of the invention separate a target nucleic acid from a sample by using at least one capture probe oligonucleotide that contains a target-complementary region and a member of a specific binding pair that attaches the target nucleic acid to an immobilized probe on a capture support, thus forming a...

20060068440 - Methods and device for dna sequencing using surface enhanced raman scattering (sers): The methods and apparatus disclosed herein concern nucleic acid characterization by enhanced Raman spectroscopy. In certain embodiments of the invention, exonuclease treatment of the nucleic acids results in the release of nucleotides. The nucleotides may pass from a reaction chamber through a microfluidic channel and enter a nanochannel or microchannel....

20060068405 - Methods and systems for annotating biomolecular sequences: Polypeptide sequences and polynucleotide sequences are provided. Also provided are annotative information concerning such sequences and uses for these sequences....

20060068410 - Methods and systems for monitoring intracellular binding reactions: Intracellular binding reactions, and particularly DNA/DNA binding protein reactions are detected in situ, using intracellular fluorescence polarization detection. The methods comprise providing a biological cell having at least a first component of a binding reaction disposed therein. The cell is contacted with a second component of the binding reaction whereby...

20060068402 - Methylated promoters of colon cancer-specific expression-decreased genes and use thereof: Methylated promoters of colon cancer-specific genes and use thereof. Various disclosed aspects of the invention include methylated promoters of the colon cancer specific expression-decreased genes, microarrays for cancer diagnosis on which the methylated promoters are immobilized, and cancer diagnosis kits containing the methylated promoters. The methylated promoters of colon cancer-specific...

20060068393 - Mevalonate kinase as a target for fungicides: The present invention relates to the provision of mevalonate kinase as target for fungicides, to the provision of novel nucleic acid sequences, of functional equivalents of the abovementioned nucleic acid sequences and to the use of the gene products of the abovementioned nucleic acid sequences as novel targets for fungicides....

20060068431 - Micro rt-pcr apparatus and method using the same: A micro RT-PCR apparatus includes a chip module and control module. The chip module includes a reaction chamber unit, and a heating unit for heating the reaction chamber unit. The control module controls the heating unit to perform a first heating operation and a second heating operation. The first heating...

20060068385 - Mononucleotide repeats microsatellite markers for detecting microsatellite instability: A method for evaluating microsatellite instability associated with a tumor, which entails the steps of amplifying microsatellite loci in a biological sample containing genomic DNA from the tumor and determining sizes of DNA amplification products, wherein at least one microsatellite locus selected from the group consisting of NR 21, NR...

20060068399 - Multiple bead reagent system for protein based assays with optimized matrices: The invention provides a multi-bead assay system for a protein based assay comprising at least two different beads. The first bead comprises protein and a protein stabilization matrix. The first bead forms a first solution when dissolved in liquid, and the first solution permits a first activity level for the...

20060068433 - Multiple mode multiplex reaction quenching method: Methods for balancing multiplexed PCR reactions are provided which exploit differences in primer and amplicon Tms. The methods may be controlled by a computer process. Also provided are articles of manufacture useful in such methods and compositions containing primers and probes useful in such methods....

20060068430 - Purification of biomolecules from contaminating intact nucleic acids: The present invention provides methods for the removal, destruction or inactivation of intact nucleic acids contaminating desired biomolecules by the use of small molecule nucleic acid cleavage agents....

20060068427 - Qualitative analysis of a sample using an algorithm: To determine the presence of a specific nucleic acid sequence within a sample, a labelled substance, capable of binding the nucleic acid to be determined, or a labelling substance is added, the substance having the ability to label the nucleic acid or being representative for the nucleic acid. The nucleic...

20060068420 - Regulation of th2 cell activity by modulation of nfatp and nfat4 activity: The invention demonstrates that NFATp and NFAT4 are required for the control of lymphocyte homeostasis and act as selective repressors of Th2 cells. The invention provides mice deficient in both NFATp and NFAT4 that exhibit a phenotype characteristic of increased Th2 cell activity. Methods for identifying modulators of Th2 cell...

20060068404 - Secreted and transmembrane polypeptides and nucleic acids encoding the same: The present invention is directed to novel polypeptides and to nucleic acid molecules encoding those polypeptides. Also provided herein are vectors and host cells comprising those nucleic acid sequences, chimeric polypeptide molecules comprising the polypeptides of the present invention fused to heterologous polypeptide sequences, antibodies which bind to the polypeptides...

20060068413 - Secreted and transmembrane polypeptides and nucleic acids encoding the same: The present invention is directed to novel polypeptides and to nucleic acid molecules encoding those polypeptides. Also provided herein are vectors and host cells comprising those nucleic acid sequences, chimeric polypeptide molecules comprising the polypeptides of the present invention fused to heterologous polypeptide sequences, antibodies which bind to the polypeptides...

20060068423 - Secreted and transmembrane polypeptides and nucleic acids encoding the same: The present invention is directed to novel polypeptides and to nucleic acid molecules encoding those polypeptides. Also provided herein are vectors and host cells comprising those nucleic acid sequences, chimeric polypeptide molecules comprising the polypeptides of the present invention fused to heterologous polypeptide sequences, antibodies which bind to the polypeptides...

20060068406 - Single-stranded nucleic acid template-mediated recombination and nucleic acid fragment isolation: Methods mediated by single-stranded nucleic acid templates, including utilizing single-stranded nucleic acid templates to isolate nucleic acid fragments and to recombine nucleic acid fragments. Methods include polymerase and polymerase-free recombination of nucleic acid fragments to generate chimeric nucleic acid sequences. Integrated systems and kits are also provided....

20060068395 - Synthetic nucleic acid molecule compositions and methods of preparation: A method to prepare synthetic nucleic acid molecules having reduced inappropriate or unintended transcriptional characteristics when expressed in a particular host cell....

20060068391 - Targeting of herpes simplex virus to specific receptors: The invention relates to engineered Herpes simplex virus (HSV) particles that are targeted to one or more specific receptors. Also, recombinant vectors for producing such HSV particles. By reducing the affinity of HSV for its natural receptor(s) and increasing the affinity for a selected receptor, the HSV particles of the...

20060068439 - Ucp4: The present invention is directed to novel polypeptides having homology to certain human uncoupling proteins (“UCPs”) and to nucleic acid molecules encoding those polypeptides. Also provided herein are vectors and host cells comprising those nucleic acid sequences, chimeric polypeptide molecules comprising the polypeptides of the present invention fused to heterologous...

20060068398 - Universal and target specific reagent beads for nucleic acid amplification: The present invention relates to methods and compositions providing optimized reaction conditions for nucleic acid amplification reactions and for methods and compositions that reduce contamination of amplification reactions during set up....

20060068409 - Use of restriction enzymes and other chemical methods to decrease non-specific binding in dual bead assays and related bio-discs, methods, and system apparatus for detecting medical targets: Methods for decreasing non-specific bindings of beads in dual bead assays and related optical bio-discs and disc drive systems. Methods include identifying whether a target agent is present in a biological sample and mixing capture beads each having at least one transport probe affixed thereto, reporter beads each having at...

20060068444 - Compositions and methods for detecting cyclic analogs of hpth: The present invention is directed to novel antigens, antibodies and antigen binding fragments thereof, and to immunoassays and kits using these antigens and antibodies. The compositions and methods are useful in the determination of levels of cyclic analogs of hPTH in sample fluids, such as serum or plasma. The antibodies...

20060068445 - Novel soluble cd14 antigen: There are provided a soluble CD14 antigen which is a novel in vivo protein useful as a marker for diagnosing sepsis and has the following characteristic features 1) to 3): 1) a molecular weight of 13±2 kDa when measured by SDS-PAGE under non-reducing conditions; 2) an amino acid sequence in...

20060068443 - Universal binding matrix for creating pairing ligands for identifying proteomic interaction: A Universal Binding Matrix is described which binds various classes of combinatorial libraries. This bound library can then be probed with test species of a simple or complex nature to identify complementary test species as well as test species surface mimics. These complementary test species have the characteristic of binding...

20060068447 - Directed assembly of functional heterostructures: The present invention relates to a systematic process for the creation of functionally organized, spatially patterned assemblies polymer-microparticle composites including the AC electric field-mediated assembly of patterned, self supporting organic (polymeric) films and organic (polymeric)-microparticle composite films of tailored composition and morphology; the present invention further relates to the incorporation...

20060068446 - Methods: A method for identifying a compound for modulating the immune response in a first type of mammal to a super-antigen, comprising the steps of 5 (7) providing a cell expressing a MHC Class II molecule derivable at least in part from the first type, or cells comprising such a cell...

20060068451 - Dimeric fluorescent polypeptides: The invention relates to proteins or polypeptides that comprise intramolecular dimers of fluorescent protein monomers. More specifically, the invention relates to recombinant polypeptides comprising a monomer of a fluorescent polypeptide, a linker peptide, and a second monomer of that fluorescent polypeptide, where the monomers form an intramolecular dimer. The invention...

20060068452 - Differential protein expression patterns related to disease states: The present invention is a method for determining protein expression profiles in disease or an altered biological state. The method is based on the use of two-dimensional (2D) gel electrophoresis where the gel images are assigned two different colors. The gels are then compared by an overlay procedure that allows...

20060068453 - Survivin, a protein that inhibits cellular apoptosis, and its modulation: The present invention provides the amino acid of a protein that inhibits cellular apoptosis, herein termed the Survivin protein and nucleic acid molecules that encode Survivin. Based on this disclosure, the present invention provides isolated Survivin protein, isolated Survivin encoding nucleic acid molecules, methods of isolating other members of the...

20060068448 - Fungal antigens and process for producing the same: There can be provided a fungal antigen which is an insoluble fraction obtainable from fungal cells of which cell wall has been substantially removed or at least partially removed; a process for producing the same; a nucleic acid encoding the fungal antigen; a biologic product containing the fungal antigen; a...

20060068449 - N-terminal and c-terminal markers in nascent proteins: This invention relates to non-radioactive markers that facilitate the detection and analysis of nascent proteins translated within cellular or cell-free translation systems. Nascent proteins containing these markers can be rapidly and efficiently detected, isolated and analyzed without the handling and disposal problems associated with radioactive reagents. Methods are described for...

20060068450 - Microbead-filled microsystem and production method thereof: The invention relates to a micro-system intended to receive beads and to obtain a precise positioning of the beads at preset locations in the micro-system. This micro-system comprises a tank that has a cavity, the cavity being fitted with blocking elements allowing the beads to be ordered and stacked in...

20060068454 - Method of inactivating blood coagulation factor and blood coagulation factor-inactivated sample: A method of inactivating a blood coagulation factor is described. The method comprises a step of contacting a sample containing at least one of factors V and VIII with a compound having an iminodiacetate group, whereby at least one of factors V and VIII in the sample is changed into...

20060068455 - Gaba detection methods and applications: Disclosed herein are silica glass sensors comprising more than one active biological material in a single sol-gel layer. The silica glass sensors may be used to assay complex biological mechanisms in cells and tissues cultivated thereon. Also disclosed are silica glass sensors having GABA-T, SSADH, or both, for detecting GAD,...

20060068456 - Methods for screening and identifying compounds: The present invention relates to methods for assessing enzyme activity utilizing a phase partition system. In addition, the present invention also relates to methods for screening and identifying compounds that may be used, for example, for the treatment of diabetes, diabetes-related disorders, obesity, cardiovascular disease, cancer, and other diseases or...

20060068457 - Substrates for tafi (a): The invention relates to compounds of general formula (I) and acid addition salts thereof, where the various symbols have the meanings given in the description and claims, the production and use thereof as substrate for the detection of TAFIa, a fibrinolysis inhibiting enzyme. The detection occurs by using the absorption...

20060068458 - Coupled enzymatic reaction system using a formate dehydrogenase derived from candida boidinii: The present process relates to a coupled enzymatic reaction system which is implemented in a two-phase system consisting of organic phase and aqueous phase. The system operates with cofactor-dependent enzymes, the cofactor being continually regenerated enzymatically by an FDH derived from C. boidinii....

20060068459 - Methods for detecting xanthrochromia: The invention provides methods and compositions for making a diagnosis of Xanthrochromia in patient-derived samples of cerebrospinal fluid....

20060068460 - Biochemical synthesis apparatus: A biochemical synthesis apparatus comprises a receptacle for containing a medium, and a support on which a microorganism can be placed. The support can be placed in contact with the medium, so as to allow access of the microorganism to the medium. The support with the microorganism can be removed...

20060068462 - Method: A method of producing one or more of a carbohydrate ester, a protein ester, a protein subunit ester or a hydroxyl acid ester, which method comprises admixing an acyl donor, an acyl acceptor and water to produce a high water environment comprising 5-98% water, wherein said acyl donor is a...

20060068461 - Transglycosylation method and protein having transglycosylation activity: The present invention provides a transglycosylation method and a protein catalyzing a transglycosylation reaction. A reaction of transferring a galactosylβ1→3N-acetylgalactosaminyl group from a sugar donor containing a sugar chain having a galactosylβ1→3N-acetylgalactosaminyl group bound thereto at the α-position, to a sugar chain acceptor, is carried out in the presence of...

20060068468 - Actrii receptor polypeptides, methods and compositions: In certain aspects, the present invention provides compositions and methods for modulating (promoting or inhibiting) growth of a tissue, such as bone, cartilage, muscle, fat, and/or neuron. The present invention also provides methods of screening compounds that modulate activity of an ActRII protein and/or an ActRII ligand. The compositions and...

20060068470 - Antifreeze proteins from basidiomy cetes: The present invention provides antifreeze proteins produced by a basidiomycete. The antifreeze protein has a high antifreeze activity such as a thermal hysteresis activity or an ice-recrystallization inhibition activity....

20060068469 - Bacterial vector systems: The present invention provides bacterial vectors and fusion proteins containing a TTSS polypeptide, compositions of such fusion proteins including polynucleotides, and methods of delivering one or more genes into a target cell that involve contacting the cell with a composition that includes such a fusion protein. Compositions and methods of...

20060068472 - Compositions to ameliorate protein misfolding and aggregation: The invention relates to polynucleotides comprising polynucleotide sequences corresponding to the tor-1, tor-2, ooc-5, DYT1, and DYT2 genes and parts thereof that encode polypeptide sequences and parts thereof possessing varying degrees of torsin activity, and methods of screening and amplifying polynucleotides encoding polypeptide sequences which encode polypeptides having varying degrees...

20060068463 - Natural killer cell activating factor ii: The invention relates to NKAF II polypeptides, polynucleotides encoding the polypeptides, methods for producing the polypeptides, in particular by expressing the polynucleotides, and agonists and antagonists of the polypeptides. The invention further relates to methods for utilizing such polynucleotides, polypeptides, agonists and antagonists for applications, which relate, in part, to...

20060068466 - Novel g protein-coupled receptor: The present invention is directed to novel G protein-coupled receptors that are found predominantly in the dorsal root ganglia. The invention encompasses both the receptor proteins as well as nucleic acids encoding the proteins. In addition, the present invention is directed to methods and compositions which utilize the receptors....

20060068464 - Regulation of human g protein coupled receptor: Reagents which regulate human G protein-coupled receptor and reagents which bind to human GPCR gene products can play a role in preventing, ameliorating, or correcting dysfunctions or diseases including, but not limited to, infections such as bacterial, fungal, protozoan, and viral infections, particularly those caused by HIV viruses, pain, cancers,...

20060068467 - Rhodococcus nitrile hydratase: The invention relates to a Rhodococcus polynucleotide cluster which contains nucleotide sequences which encode polypeptides having the activity of a nitrile hydratase, of an auxiliary protein P15K which activates this enzyme and of a cobalt transporter, to transformed microorganisms in which the nucleotide sequences encoding these proteins are present in...

20060068471 - Soluble zcytor 11 cytokine receptors: Novel polypeptide combinations, polynucleotides encoding the polypeptides, and related compositions and methods are disclosed for soluble zcytor11 receptors that may be used as novel cytokine antagonists, and within methods for detecting ligands that stimulate the proliferation and/or development of hematopoietic, lymphoid and myeloid cells in vitro and in vivo. Ligand-binding...

20060068465 - Ubiquitin-specific protease occurring in the brain and dna encoding the same: A ubiquitin-specific protease occurring in the brain and a DNA encoding it, which are useful for research on the molecular mechanism of the neuroplasticity expression and so on....

20060068473 - Lactobacillus n-deoxyribosyl transferases, corresponding nucleotide sequences and their uses: The invention concerns novel polypeptides and their fragments, isolated from Lactobacillus, having at least a N-deoxyribosyl transferase activity, the polynucleotides encoding said polypeptides, cloning and/or expression vectors including said polynucleotides, cells transformed by said vectors and specific antibodies directed against said polypeptides. The invention also concerns a method for enzymatic...

20060068474 - Endothelial-monocyte activating polypeptide iii: A human EMAP III polypeptide and DNA (RNA) encoding such polypeptide and a procedure for producing such polypeptide by recombinant techniques is disclosed. Also disclosed are methods for utilizing such polypeptide for preventing and/or treating neoplasia. Diagnostic assays for identifying mutations in nucleic acid sequence encoding a polypeptide of the...

20060068475 - Pre-treatment of bales of feedstock: The present invention is directed to a method of pre-treating a lignocellulosic feedstock. The lignocellulosic feedstock comprises cereal straw, stover, or grass. One or more than one bale of lignocellulosic feedstock is conveyed into a pre-treatment reactor. Steam and acid are added to the bales and are maintained at a...

20060068476 - Method for the production by fermentation of sulphur-containing fine chemicals (metf): The invention relates to methods for the fermentative production of sulfur-containing fine chemicals, in particular L-methionine, by using bacteria which express a nucleotide sequence coding for a methionine synthase (metF) gene....

20060068477 - Methods for the production of products in host cells: The invention provides methods and host cells for the production of ascorbic acid intermediates. The invention also provides host cells having a modification in a polynucleotide that uncouples the catabolic pathway from the oxidative pathway by deleting the encoding for an endogenous enzymatic activity that phosphorylates D-glucose at its 6th...

20060068479 - Porcine forssman synthetase protein, cdna, genomic organization, and regulatory region: The present invention provides porcine Forssman synthetase (FSM synthase) (Globoside α-N-acetylgalactosaminyltransferase) protein, cDNA, and genomic DNA sequence. Furthermore, the present invention includes porcine animals, tissue and organs as well as cells and cell lines derived from such animals, tissue and organs, which lack expression of functional FSM synthetase. Such animals,...

20060068478 - Site specific incorporation of keto amino acids into proteins: Compositions and methods of producing components of protein biosynthetic machinery that include orthogonal tRNAs, orthogonal aminoacyl-tRNA synthetases, and orthogonal pairs of tRNAs/synthetases, which incorporate keto amino acids into proteins are provided. Methods for identifying these orthogonal pairs are also provided along with methods of producing proteins with keto amino acids...

20060068480 - Polypeptides and polypeptide analogues: m

20060068481 - Human kinases: The invention provides human kinases (PKIN) and polynucleotides which identify and encode PKIN. The invention also provides expression vectors, host cells, antibodies, agonists, and antagonists. The invention also provides methods for diagnosing, treating, or preventing disorders associated with aberrant expression of PKIN....

20060068483 - Extracellular serine protease: The present invention provides a DNA encoding a TADG-14 protein selected from the group consisting of: (a) isolated DNA which encodes a TADG-14 protein; (b) isolated DNA which hybridizes to isolated DNA of (a) above and which encodes a TADG-14 protein; and (c) isolated DNA differing from the isolated DNAs...

20060068482 - Taspase1 and methods of use: A novel protease, Taspase1, is described. Taspase1 is involved in the cleavage of the MLL protein, which is required for proper HOX gene regulation. Diagnostic methods utilizing Taspase1 are provided, as well as inhibitors of Taspase1. Methods of using the inhibitors of Taspase1 are also described. For example an inhibitor...

20060068484 - Enteropathogenic e. coli is a separate genus pathogenic bacteria: The invention is intended for treatment of diseases in accordance with immunity deficiency (HIV/AIDS diseases and other), oncological ailments, diseases connected with aggresive autoantibody (Diabetes and other autoimmune diseases), tuberculosis and etc. Purpose of the invention is the reinforcement of immunological reactivity organism. To this effect it is offered to...

20060068485 - Novel human synaptotagmin and polynucleotides encoding the same: Novel human polynucleotide and polypeptide sequences are disclosed that can be used in therapeutic, diagnostic, and pharmacogenomic applications....

20060068486 - Pet waste disposal assembly and method: An assembly adapted to facilitate the disposing and treating of a biodegradable material that produces an unpleasant odor when it is watered and stirred and fragmented. The assembly includes a container and a fitting for accepting and retaining the insertive coupler of a standard garden hose. In addition, a pressure...

20060068489 - Apparatus and method of assay in utilizing attenuated total reflection: An assay apparatus is loaded with a sensor unit as surface plasmon resonance (SPR) biosensor, which includes a transparent dielectric medium and thin film. A first surface of the thin film is a thin film/dielectric interface. Its sensing surface causes reaction of sample fluid. An optical assay unit assays reaction...

20060068487 - Nanostructured surface for microparticle analysis and manipulation: The present invention provides an apparatus, comprising a first mechanical structure having a first rigid surface, an area of the first rigid surface having a nanostructured surface. The apparatus also includes a second mechanical structure having a second rigid surface and opposing the first mechanical structure. The second rigid surface...

20060068488 - Screening apparatus: A screening apparatus detects images of a plurality of wells provided at a plate to perform screening in a new drug process. The screening apparatus has a plurality of detecting portions which are fixedly arranged and individually detect images of wells different from each other, a movable stage on which...

20060068490 - Flow-through chemical and biological sensor: The invention provides a mixing flow apparatus. The mixing flow apparatus consists of a waveguide and a mixing flow chamber; the waveguide having a higher index of refraction material than its surroundings for propagation of a signal, and the mixing flow chamber having a body forming a flow chamber with...

20060068491 - Microdevice for performing method of separating and purifying nucleic acid: A microdevice for performing a method for separating and purifying a nucleic acid, the microdevice comprising: at least one opening; and at least one channel for passing a sample solution, wherein the method comprises: (A) a step of bringing a nucleic acid-containing sample solution into contact with a nucleic acid-adsorbing...

20060068492 - Hybrid bioreactor for cell culture: A hybrid bioreactor for cell culture is disclosed. To simultaneously apply compressive strain for cell differentiation and shear strain for cell proliferation to cells, the hybrid bioreactor includes a plurality of reactor tube assemblies (100), a compressive strain motor (5), a shear strain motor (25), a lower anchor mount (20)...

20060068493 - Protein activity screening of clones having dna from uncultivated microorganisms: Disclosed is a process of screening clones having DNA from an uncultivated microorganism for a specified protein, e.g. enzyme, activity by screening for a specified protein, e.g. enzyme, activity in a library of clones prepared by (i) recovering DNA from a DNA population derived from at least one uncultivated microorganism;...

20060068494 - Wnt4 in supporting lymphopoiesis: The T lineage differentiative potential of lymphoid progenitor cells in the lymph nodes is thwarted by the absence of Wnt signaling, resulting in blockade of the DN1→DN2 transition with accumulation of pre-DN2 cells. Wnt4 transcripts are deficient in the lymph nodes relative to the thymus. When cultured with stromal cells...

20060068495 - Process of cell electrofusion: The invention relates to the use of an electrical field applied to a mixture containing a first type of cell (C1), a second type of cell (C2), a bispecific ligand able to bind to C1 and/or to C2 and non-covalent complexes formed between C1, C2 and the bispecific ligand, for...

20060068496 - Differentiation of stem cells: Disclosed are compositions and methods for identifying specific cell types....

20060068497 - Methods for isolating novel antimicrobial agents from hypermutable mammalian cells: Dominant-negative alleles of human mismatch repair genes can be used to generate hypermutable cells and organisms. By introducing these genes into mammalian cells new cell lines with novel and useful properties can be prepared more efficiently than by relying on the natural rate of mutation or introduction of mutations by...

20060057552 - Method of conserving biological prostheses, conserved biological prostheses and conserving solution: The invention concerns a method of conserving biological prostheses, wherein the method includes the following steps: (a) treating biological prostheses with a solution which contains a mixture of epoxide compounds which are at least in part of different lengths; (b) treating the biological prosthesis treated in accordance with step (a)...

20060057553 - Chimeric viral vectors for gene therapy: The invention relates to a single nucleic acid vector comprising both adenoviral and retroviral sequences for gene therapy. The vectors described herein (See FIG. 2) are capable of transducing all cis and trans components of a retroviral vector for the generation of high titer recombinant retroviral vectors. The chimeric vectors...

20060057556 - Contiguous capillary electrospray sources and analytical devices: Contiguous capillaries useful for separating and electrospraying a fluid comprising analyte and electrolyte are provided. The contiguous capillaries have spray tips at one end of the capillaries and electrically conductive portions in proximity to the spray tips. Methods for making the contiguous capillaries and their use as electrospray sources are...

20060057559 - High-throughput cell migration screening assay: The invention relates to methods for identifying and using agents, including small organic molecules, antibodies, peptides, cyclic peptides, nucleic acids, antisense nucleic acids, sphingolipid analogs, and ribozymes, that modulate cell activation or migration, e.g., lymphocyte migration, via modulation of the expression and/or activity of migration molecules such as, for example,...

20060057555 - Method and system for controlling the development of biological entities: A method and system are presented for use in controlling the processing of components, e.g. biological entities. Each component is assigned with a unique machine readable identification mark. Data records are provided representative of matching sets of the identification marks relating to at least two associated components. Each component-containing holder...

20060057557 - Method for identifying an image of a well in an image of a well-bearing component: A method for identifying images of wells in an image of a well-bearing object such as multiwell plates or picowell carriers is provided. An observation component, such as a camera, is used to approach focus of a focal point of a well-bottom. An image of the focal point is acquired....

20060057558 - Method to measure the effect of topically applied agents using skin maintained in organ culture: The method employs a piece of skin of area greater than 1 cm2 cultured under conditions that maintain its viability and substantially normal structure for sufficient time for topically applied test material to potentially exert an effect. The skin includes the majority of the epidermal layer plus an appropriate amount...

20060057554 - Sample collecting device and mass spectrometry of device: A sample collection device comprising a support bearing an inert absorbing matrix for a fluid sample is described. The device may or may not have a lancet. Also described for a sample device is a method of using a mass spectrometer in a laboratory where the sample in its matrix...

20060057560 - System and method for denaturing and fixing collagenous tissue: A method for modifying a geometry of a collagenous tissue mass includes heating the collagenous tissue mass to a temperature sufficient to cause denaturation, and introducing a biocompatible fixative, such as genepin, into the collagenous tissue mass....

20060057562 - Method, composition and kit for antigenic binding of norwalk-like viruses: A method for detecting a Norwalk-Like Virus (NLV) in a biological sample, comprising the steps of: obtaining a biological sample suspected of containing a NLV; contacting the biological sample with at least one human histo-blood group antigen to allow formation of a complex of the NLV with the antigen; and...

20060057563 - Peptides derived from capsid antigens of the epstein-barr virus and the use thereof: In the area of virus diagnosis, in particular Epstein-Barr virus (EBV) diagnosis, methods for the detection of EBV and agents suitable for this purpose are provided, including peptides which are derived from p18-VCA and permit discrimination between acute and past EBV infection....

20060057561 - Virus detection method, primers therefor and screening kit: Discloses a novel detection and typing method for viruses, such as human papillomaviruses, based on real-time PCR using self-probing amplicon fluorescent primers. The method comprises: (IA) contacting the sample with a self-probing amplicon (‘virus self-probing amplicon’) comprising (i) a virus primer capable of hybridising to at least one target viral...

20060057582 - 83 human secreted proteins: The present invention relates to novel human secreted proteins and isolated nucleic acids containing the coding regions of the genes encoding such proteins. Also provided are vectors, host cells, antibodies, and recombinant methods for producing human secreted proteins. The invention further relates to diagnostic and therapeutic methods useful for diagnosing...

20060057596 - Activity-based assay for ricin-like toxins: A method of detecting N-glycosylase activity in a sample involves incubating an oligodeoxyribonucleotide substrate containing a deoxyadenosine or deoxyuridine residue with the sample to be tested such that the N-glycosylase, if present, hydrolyzes the deoxyadenosine or deoxyuridine residue to result in an N-glycosylase product having an abasic site. A primer...

20060057624 - Alternatively spliced pre-mrna transcripts in neurodegenerative disease: The present invention provides a method of diagnosing a neurodegenerative disease in a mammalian subject, preferably a human subject. The method comprises obtaining RNA from the mammalian subject, and assaying the RNA for an increase in the amount of ΔFosB mRNA or in ratio of the amount of ΔFosB mRNA...

20060057617 - Amplification process: A method for conducting a nucleic acid amplification reaction, said method comprising forming an amplification reaction mixture in the presence of sufficient of a pyrophosphate salt to prevent primer extension taking place, digesting said pyrophosphate salt with a pyrophosphatase enzyme (PPase), and subjecting said reaction mixture to conditions such that...

20060057570 - Antisense modulation of hematopoietic cell protein tyrosine kinase expression: Antisense compounds compositions and methods are provided for modulating the expression of hematopoietic cell protein tyrosine kinase. The compositions comprise antisense compounds, particularly antisense oligonucleotides, targeted to nucleinc acids encoding hematopoietic cell protein tyrosine kinase. Methods of using these compounds for modulation of hematopoietic cell protein tyrosine kinase expression and...

20060057621 - Assay for detecting tuberculosis in nonhuman primates: Systems, methods, and compositions for a diagnostic method for detecting TB in non-human primates that is easy to use, sensitive, and specific. The method utilizes recombinant mycobacterial antigens, such as polyfusion proteins. The method utilizes an antigen-antibody-antigen arrangement to detect TB infection in nonhuman primates. The method can detect IgM...

20060057626 - Assessment of ctla-4 polymorphisms in ctla-4 blockade therapy: The invention provides methods for predicting responsiveness of a subject to therapy with a CTLA-4 blocking agent. The methods involve assaying for at least one CTLA-4 polymorphism in the subject and predicting responsiveness of the subject to therapy with a CTLA-4 blocking agent based on presence or absence of a...

20060057580 - Biochip with maximization of the reactor number: This invention concerns a biochip, consisting primarily of one or several dieplates and one or several substrates with or without probe immobilized, and comprising a maximized number of reactors, wherein: a. said maximization of said reactor number is performed by minimizing structure-covered area on the substrate and/or maximizing effective area...

20060057589 - Biomarkers for toxic algae: The present invention is directed toward biomarkers that identify characteristics of algae. The invention is further directed toward biomarkers that serve to identify algae species and strains of algae species as well as detect the presence of algal toxins. Additional embodiments feature methods utilizing algal biomarkers and polypeptides that can...

20060057565 - Combinatorial fluorescence energy transfer tags and uses thereof: This invention provides a combinatorial fluorescence energy transfer tag which comprises a plurality of fluorescent molecules, comprising one or more energy transfer donor and one or more energy transfer acceptor, linked through a molecular scaffold wherein the fluorescent molecules are separated along the scaffold to produce a unique fluorescene emission...

20060057605 - Compositions for use in identification of viral hemorrhagic fever viruses: The present invention provides oligonucleotide primers, compositions, and kits containing the same for rapid identification of viruses that cause viral hemorrhagic fevers by amplification of a segment of viral nucleic acid followed by molecular mass analysis....

20060057595 - Compositions, methods, and kits for identifying and quantitating small rna molecules: Compositions, methods, and kits for identifying and for quantitating polynucleotide targets are disclosed. These compositions, methods, and kits are particularly useful when the polynucleotide target is a small RNA molecule, including without limitation microRNA (miRNA), small interfering RNA (siRNA), and certain other classes of non-coding RNA molecules. The forward and...

20060057603 - Detecting bacillus anthracis: The present invention utilizes unique DNA signatures to identify Bacillus anthracis. The DNA signatures can also be used in other types of detection. These signatures have been found to be unique to Bacillus anthracis, and can be used to understand how these DNA regions are being used to make this...

20060057578 - Determination of an analyte in a liquid medium: The present invention concerns a magneto-controlled method and system for the determination of an analyte in a liquid medium. The method and system of the invention are based on the use of functionalized magnetic particles, e.g. magnetic particles that carry a recognition agent, such that in the presence of the...

20060057618 - Determining data quality and/or segmental aneusomy using a computer system: A method and/or system for making determinations regarding samples from biologic sources including statistical methods for making meaning grouping of observed data and/or for determining an overall quality measure of an assay....

20060057629 - Device for injecting pcr solution into pcr channels of pcr chip, and pcr chip unit including the device: Provided is a device for injecting a PCR solution into PCR channels of a PCR chip. The device includes: first through-holes corresponding to each of the inlets of the PCR chip in which PCR channels are formed; and second through-holes corresponding to each of the outlets of the PCR chip....

20060057577 - Diagnostics and therapeutics for diseases associated with homo sapiens formyl peptide receptor-like 2: The invention provides a human FPRL2 which is associated with the cardiovascular diseases, cns disorders, hematological diseases, genito-urinary diseases, cancer and respiratory diseases. The invention also provides assays for the identification of compounds useful in the treatment or prevention of cardiovascular diseases, cns disorders, hematological diseases, genito-urinary diseases, cancer and...

20060057584 - Endometriosis-related markers and uses thereof: The present invention relates to markers of endometriosis which are differentially expressed in the endometrial cells of females with endometriosis compared to endometriosis-free females. The invention also relates to methods for determining likelihood of endometriosis in female subjects, to methods for grading endometriosis in females suffering from endometriosis and to...

20060057601 - Genomic markers of hepatitis b virus associated with hepatocellular carcinoma: The present invention provides methods of predicting a pre-disposition of HBV-infected individuals to develop hepatacellular carcinoma (HCC)....

20060057564 - Identification and mapping of single nucleotide polymorphisms in the human genome: The invention relates to the role of genes in human diseases. More particularly, the invention relates to compositions and methods for identifying genes that are involved in human disease conditions. The invention provides identification and mapping of a very large number of SNPs throughout the entire human genome. This contribution...

20060057611 - Log-linear amplification: The present disclosure provides methods and composition to detect or quantitate one or more target sequences in a reaction that couples a linear amplification reaction to an exponential amplification reaction....

20060057622 - Low cycle amplification of rna molecules: The compositions and methods afford an almost unlimited linear RNA amplification from a few cells with minimal differences in the relative abundance of amplified RNAs and their parent mRNA (sample distortion)....

20060057597 - Method and system for processing nanoparticles using a self assembly mechanism to form combined species: A method for processing nanoparticles using a self assembly mechanism. The method includes flowing a first reactant species through a first channel region, which has a predetermined dimension including a first width and a first depth. The method includes flowing a second reactant species through a second channel region, which...

20060057567 - Method for constructing a chimeric dna library using a single strand speific dnase: The method for constructing a chimeric DNA library of the present invention uses matching regions of heterologous DNA sequences as internal primers and enables highly efficient DNA reassembly between heterologous DNAs having relatively low similarity. Therefore, the inventive method can be effectively used for developing a useful gene having better...

20060057593 - Method for detecting cystic fibrosis: The present invention relates to methods for amplifying various regions of the cystic fibrosis transmembrane regulator (CFTR) gene. Methods are provided for amplifying one or all 27 exons of the CFTR gene and a portion of the CFTR promoter region in a single tube. The method can identify the presence...

20060057569 - Method for detecting hybridization events in nucleic acids: The invention relates to materials and methods for detecting the presence of and/or quantifying a target polynucleotide in a sample by using probe polynucleotides attached to the surface of an array, which probes are at least partially complementary to the target polynucleotide. A kit for carrying out the invention is...

20060057613 - Method for distinguishing methicillin resistant s. aureus from methicillin sensitive s. aureus in a mixed culture: The present invention provides isolated oligonucleotides and methods for detecting a methicillin resistant Staphylococcus aureus in a sample, including a sample that comprises nucleic acid molecules of higher biological complexity than that of amplified nucleic acid molecules....

20060057604 - Method for electrically detecting oligo-nucleotides with nano-particles: A method for detecting a target oligo-nucleotides includes the following steps: providing a substrate mounted with at least a pair of detecting electrodes separated with a gap; coating a surface activation agent on the substrate; providing a plurality of nano-particles and immobilizing them between the two detecting electrodes on the...

20060057579 - Method for predicting a drug transport capability by abcg2 polymorphisms: The present invention relates to a polypeptide which excretes drugs such as cancer chemotherapeutic agents from a cell and to a gene coding therefor. More specifically, the present invention relates to a method for predicting a drug transport capability of a mammalian cell by determining a single nucleotide polymorphism(s) of...

20060057591 - Method for preventing viral infection: There is provided a method for preventing or treating viral infections using members of the annexin proteins family. In particular annexins 1 and 5 as well as inhibitors of heterotetrameric annexin 2, such as antibodies, significantly reduce the production of virus progeny....

20060057628 - Method for prognosticating the progress of breast cancer and compounds useful for prevention or treatment thereof: This invention concerns in vitro method for prognosticating the progress of breast cancer, comprising detecting or quantifying the level of 17β-hydroxysteroid dehydrogenase (17HSD) type 1 enzyme in breast tumor tissue sample, wherein the presence of said 17HSD type 1 enzyme is indicative of severe progress of breast cancer. Furthermore, the...

20060057575 - Method for two-dimensional conformation-dependent separation of non-circular nucleic acids: Methods are provided for separating linear nucleic acid fragments based on their conformation. The methods are based on novel two-dimensional gel electrophoresis techniques. In one aspect the method comprises electrophoresing a sample of nucleic acids in a first dimension said sample through a gel matrix under a first set of...

20060057568 - Method of identifying compounds that alter bag-1 mediated down-regulation of glucocorticoid-receptor transactivation: The present invention relates to new methods of identifying compounds that inhibit or reduce bag-1 mediated down-regulation of gluocorticoid-receptor (GR) tansactivation. These methods rely on the surprising findings, that first, a (poly)peptide comprising the motif X-Lys-Lys-Lys-Y-Arg-Arg-Arg also present is the cochaperone bag-1 is sufficient for DNA binding of this cochaperone...

20060057588 - Method of separating tumor cells with and without lymphotropic metastatic potential in a human carcinoma: A method for separating tumor cells with lymphotropic metastatic potential from those without lymphotropic metastatic potential in a human carcinoma. Cells of the carcinoma are transplanted in each of a plurality of fresh athymic mice. At least one of the athymic mice which does not develop a palpable tumor at...

20060057572 - Method of testing food: A method for detecting the presence/absence of a specific substance in foods by performing PCR using primers which are designed on the basis of data obtained from a part of a gene of the specific substance. This method is highly useful in detecting a trace component contained in a food...

20060057566 - Methods and compositions for analyzing nucleic acid molecules utilizing sizing techniques: Tags and linkers specifically designed for a wide variety of nucleic acid reactions are disclosed, which are suitable for a wide variety of nucleic acid reactions wherein separation of nucleic acid molecules based upon size is required....

20060057573 - Methods and reagents for detecting target binding by nucleic acid ligands: The present invention provides novel methods and reagents for detecting the binding of protein targets to nucleic acid ligands. Using Universal Protein Stains (UPS), proteins bound by nucleic acid ligands may be labeled with a detectable moiety. The methods and reagents are particularly useful for the detection of protein targets...

20060057600 - Methods for detecting dna viruses: A DNA virus in a sample is detected by contacting the sample with host cells that have been transiently transfected with a reporter sequence under the control of a virus-specific promoter, and then detecting the reporter....

20060057571 - Methods for detecting nucleic acids while using solid phase bound primers and cleaving the products of a cyclic amplification reaction (trap release primer amplification (tramp): The invention relates to methods for detecting nucleic acids with the aid of an amplification reaction whereby involving a novel structure of solid phase bound starter molecules (primers) with a cyclic amplification reaction on the solid phase and a transformation of the amplification products into the aqueous phase. The solid...

20060057612 - Methods for diagnosing osteoporosis or a susceptibility to osteoporosis based on haplotype association: Methods for diagnosis of osteoporosis or a susceptibility to osteoporosis based on detection of at risk haplotypes associated with BMP2 are disclosed....

20060057592 - Methods for identifying primase trinucleotide initiation sites and identification of inhibitors of primase activity: Methods and kits for the identification of a primase trinucleotide initiation site and for the identification of compounds which modulate bacterial primase activity are provided....

20060057609 - Methods for predicting methotrexate polyglutamate levels using pharmacogenetics: The present invention provides methods for determining a level of methotrexate polyglutamates (MTXPGs) in an individual undergoing methotrexate (MTX) therapy and for optimizing dose efficacy of MTX therapy in an individual by genotyping the individual at a polymorphic site in at least one folate pathway gene (e.g., a reduced folate...

20060057576 - Microcapillary hybridization chamber: The invention provides a microcapillay hybridization chamber made of a narrow bore tubing with probe segments. Each probe segment has oligonucleotide probes covalently attached to the inner wall of the tubing and the oligonucleotide probes within each segment have identical, known sequences. Many oligonucleotide probe segments can be present within...

20060057581 - Microfabricated fluidic device for fragmentation: A microfabricated device for fragmenting nucleic acids present in a fluid sample, the device comprising an inlet port (20), a fragmentation cell (1), and an outlet port (10) downstream from said inlet port (20), said cell (1) being in fluid communication with said ports, and wherein said outlet port is...

20060057630 - Mll translocations specify a distinct gene expression profile, distinguishing a unique leukemia: The present invention relates to the diagnosis of mixed lineage leukemia (MLL), acute lymphoblastic leukemia (ALL), and acute myelogenous leukemia (AML) according to the gene expression profile of a sample from an individual, as well as to methods of therapy and screening that utilize the genes identified herein as targets....

20060057619 - Mutant met and uses therefor: The present invention provides compositions and methods for detecting mutations in the proto-oncogene Met....

20060057631 - Nano-structured device for analysis or separation, and its preparation and application: This invention involves the nano-structured support used for separation or/and analysis, especially the chip substrate, ELISA plate substrate, planar chromatography strip and chromatography gel. Besides, it involves the functionalized nano-structured support of high sensibility for separation or/and analysis, especially the analysis-chip, ELISA plate, planar chromatography reagent strip and chromatography gel....

20060057585 - Nanostepper/sensor systems and methods of use thereof: Nanostepper/sensor systems and methods for analyzing a polymer are provided....

20060057583 - Novel compositions and methods for controlling the extendability of various components used in copying or amplification steps: This invention relates to novel compositions and methods for controlling the extendability of various components used in copying or amplification steps to provide an enhancement of the specificity of the nucleic acids produced by these processes as well as potentially increasing the amount of such products. By these means, the...

20060057587 - Oligonucleotide arrays comprising background probes: The invention relates to chemical arrays comprising a plurality of features comprising biopolymers at known locations on the substrate, wherein at least one feature comprises specific hybridization probes designed or selected to bind to specific target sequences and background probes for evaluating non-specific binding of target. Methods, systems, and kits...

20060057594 - Preconditioning for eda cell library: A characterized cell library for EDA tools includes one or more mathematical models for each cell, and one or more preconditioning functions (and/or inverse preconditioning functions) for each mathematical model. Each mathematical model represents a performance parameter (e.g., delay, power consumption, noise) or a preconditioned performance parameter of the cell....

20060057608 - Producing, cataloging and classifying sequence tags: The described method provides, methods, and kits to produce, identify, catalog and classify a comprehensive collection of nucleic acid targets produced from a nucleic acid sample. The method, referred to as Cataloging and Classification of Sequence Tags, involves generating a set of target nucleic acid fragments; coupling the target nucleic...

20060057606 - Reagents containing terminal-phosphate-labeled nucleotides for nucleic acid sequencing: The present invention is directed to a method of sequencing a target nucleic acid molecule having a plurality of bases. In its principle, the temporal order of base additions during the polymerization reaction is measured on a molecule of nucleic acid, i.e. the activity of a nucleic acid polymerizing enzyme...

20060057615 - Risk prediction for hypertension, elevated plasma triglyceride and metabolic syndrome: The lipoprotein lipase (LPL) gene is associated with hypertension, elevated plasma triglyceride and metabolic syndrome. A haplotype in the intron 3-intron 4 region (the C/AA/T haplotype), and a haplotype in the intron 5-intron 6 region (the GTAG haplotype), are particularly useful in predicting the risk of an individual for these...

20060057590 - Rna probes: The invention is based in part on the generation of a double stranded RNA molecule substantially covering the whole transcribed region of a gene, and cleaving this using an RNA endonuclease to generate small RNA molecules which are already or may be subsequently labelled. The invention provides small labelled ribonucleic...

20060057602 - Rtvp based compositions and methods for the treatment of prostate cancer: This invention relates to a gene encoding RTVP that has been shown to be up-regulated by p53 using differential display-PCR and subsequently by co-transfection studies. RTVP-1 mRNA is abundant in normal mouse and human prostatic epithelial cells and primary tumors, but is significantly down regulated in metastatic mouse and human...

20060057625 - Scaffold-based artificial receptors and methods: The present invention relates to scaffold artificial receptors, methods of and compositions for making them, and methods of using them. Each artificial receptor includes a plurality of building blocks. The plurality of the building blocks are coupled to a scaffold....

20060057627 - Selection scheme for enzymatic function: The present invention includes compositions and methods of selecting functional genetic elements that includes selecting in vivo an autogene operon with one or more mutagenized functional genetic elements and one or more selectable elements, wherein survival of the autogene depends on expression of the one or more mutagenized functional genetic...

20060057623 - Senp1 as a marker of cancer development and target for cancer therapy: The present invention relates to compositions and methods for the use of SENP1 as a marker for cancer diagnosis, specifically prostate cancer. Still further, it relates to the use of inhibitors of SENP1 to inhibit cell proliferation of a neoplasm or tumor cell and to treat a hyperproliferative disease....

20060057616 - Sensitive detection of bacteria by improved nested polymerase chain reaction targeting the 16s ribosomal rna gene and identification of bacterial species by amplicon sequencing: A method for identifying an RNA form of a bacteria, comprising reverse transcribing RNA material; conducting PCR using primers for a first highly conserved genetic sequence generic of the bacteria; conducting nested PCR using primers for a second highly conserved genetic sequence within the first genetic sequence of the bacteria;...

20060057607 - Small rnas and bacterial strains involved in quorum sensing: Quorum-sensing bacteria communicate with extracellular signal molecules called autoinducers to allow community-wide synchronization of gene expression. The present invention relates to the identification the Vibrio harveyi and Vibrio cholerae protein Hfq as mediating interactions between small, regulatory RNAs (sRNAs) and specific messenger RNA (mRNA) targets. Accordingly, the present invention provides...

20060057599 - System for autonomous monitoring of bioagents: An autonomous monitoring apparatus for monitoring air, water, soil, or other substance for bioagents. A collector gathers a quantity of the air, water, soil, or other substance being monitored. A sample preparation system prepares a sample of the selected potential bioagent particles. The sample is analyzed by a system for...

20060057610 - System for producing synthetic promoters: The present application discloses a method for making and selecting a transcription enhancing combined promoter cassette, which includes constructing a library of randomly combined transcription regulatory elements, which comprise double stranded DNA sequence elements that are recognized by transcription regulators; inserting the combined transcription regulatory elements upstream of a minimum...

20060057614 - Tethering neuropeptides and toxins for modulation of ion channels and receptors: The present invention provides a method for tethering peptides, including neuropeptides and peptide toxins, including naturally occurring and mutant or altered peptides, to a surface, including the cell surface. The tethered peptides are not naturally tethered or attached to a surface, including a cell surface, or through a cell membrane....

20060057574 - Tissue specific expression: The present invention relates to a synthetic regulatory element for tissue specific gene expression. Furthermore, the invention relates to a tumor cell specific expression system for the expression of therapeutic genes as well as a pharmacological composition....

20060057586 - Transcript mapping method: A transcript mapping method according to an embodiment of the invention is described hereinafter and combines short tag based (SAGE and MPSS) efficiency with the accuracy of full-length cDNA (flcDNA) for comprehensive characterization of transcriptomes. This method is also referred to as Gene Identification Signature (GIS) analysis. In this method,...

20060057620 - Treatment of hiv infection: A method of treating HIV infection comprises administering to a patient a regimen that is able to shut down the immune system in a controlled manner by using T-cell depletion or T-cell modification such that T-cells no longer can be attacked by HIV cells. The T-cell depletor or T-cell modifiers...

20060057598 - Use of microphthalmia for diagnosis, prognosis and/or treatment of melanoma: Microphthalmia (Mi) while present in melanocytes, a cells and osteoclast, is not normally present in other cells. We have found that Mi is present in the nucleus of melanoma cells. Melanoma can be diagnosed by contacting a malignant cell with a probe for Mi. If the probe identifies Mi in...

20060057641 - Allergenic proteins and peptides from dog dander and uses therefor: Isolated nucleic acids encoding allergens of Canis familiaris, Canf I or Can f II. are disclosed. A cDNA encoding a peptide having a Can f I activity and a predicted molecular weight of about 19,200 daltons is also described. A cDNA encoding a peptide having Can f II activity and...

20060057632 - Artificial antibody library with super-repertory: An artificial antibody library with a super-repertory (1011 or more) is constructed by: using a cDNA library as a template, amplifying a fragment containing the CDR1 and CDR2s regions of the VH or VL region of immunoglobulin gene and a fragment containing the CDR3 region each by the PCR method;...

20060057634 - Assay for glycosylated proteins: The invention provides a method for assaying for a protein having at least two isoforms having different glycosylation patterns, said method comprising: contacting a sample containing said protein with a carbohydrate-binding agent and a ligand capable of binding to at least two said isoforms, and detecting conjugates of said carbohydrate-binding...

20060057639 - Camkii/calcium channel phosphorylation-related compositions and methods: This invention provides a method for determining whether an agent inhibits phosphorylation of a calcium channel by CaMKII. Furthermore, this invention provides a method of inhibiting phosphorylation of a calcium channel by CaMKII in a cell. Finally, this invention provides methods for treating cardiac arrhythmia and neurological disorders....

20060057635 - Coated metal surface on solid support for displacement reactions: A coated metal surface on a solid support, wherein the coating consists of a self-assembled monolayer (SAM) of oligo(ethylene glycol)-terminated amide group-containing alkyl thiols firmly attached to the metal surface via the thiol-end and low molecular weight antigens bound via an amide-group to the SAM-forming OEG molecule, wherein the alkyl...

20060057636 - Composite peptide compounds for diagnosis and treatment of diseases caused by prion proteins: The present invention relates to diseases caused by prion proteins, Novel composite peptide compounds are disclosed which comprise two or more peptides or peptide fragments optionally linked to a backbone and the peptides or peptide fragments are spatially positioned relative to each other so that they together form a non-linear...

20060057644 - Compositions and methods for stabilizing transthyretin and inhibiting transthyretin misfolding: Kinetic stabilization of the native state of transthyretin is an effective mechanism for preventing protein misfolding. Because transthyretin misfolding plays an important role in transthyretin amyloid diseases, inhibiting such misfolding can be used as an effective treatment or prophylaxis for such diseases. Treatment methods, screening methods, as well as specific...

20060057642 - Disease markers for early stage atherosclerosis: The invention relates to methods of diagnosing early stages of atherosclerosis....

20060057637 - Inorganic particle conjugates: The ionic conjugates include an inorganic particle electrostatically associated with a macromolecule which can interact specifically with predetermined chemical species or biological targets....

20060057643 - Methods and compositions for detecting erythrovirus genotypes: The invention provides methods and compositions for rapid, sensitive, and highly specific nucleic acid-based (e.g., DNA based) detection of human erythroviruses, such as the B19, V9, and A6 genotype, in a sample. In general, the methods involve detecting a target nucleic acid having a target sequence of a conserved region...

20060057638 - Methods and products related to the improved analysis of carbohydrates: The invention relates, in part, to the improved analysis of carbohydrates. In particular, the invention relates to the analysis of carbohydrates, such as N-glycans and O-glycans found on proteins. Improved methods, therefore, for the study of glycosylation patterns on cells, tissue and body fluids are also provided. Information regarding the...

20060057633 - Methods of creating modified promoters resulting in varying levels of gene expression: The present invention relates to a method of creating promoter cassettes that include modified precursor promoters and transforming a population of bacterial host cells with a promoter library comprising the promoter cassettes resulting in bacterial clones having a range of expression levels of a gene of interest. The invention further...

20060057640 - Modulators of odorant receptors: The present invention relates to polypeptides capable of promoting odorant receptor cell surface localization and odorant receptor functional expression. The present invention further provides assays for the detection of ligands specific for various odorant receptors. Additionally, the present invention provides methods of screening for odorant receptor accessory protein polymorphisms and...

20060057646 - Diagnosis of alzheimer's disease: A method of diagnosis of Alzheimer's disease comprising the steps of obtaining a sample selected from of a group of samples consisting of body fluid samples and tissue homogenate samples from a patient; of exposing the sample to an antibody directed against Amyloid-beta (Aβ) peptides; of separating a fraction of...

20060057645 - Novel genes: The object of the present invention is to detect and select insulin-producing cells capable of proliferating, and further to differentiate/proliferate insulin-producing cells and precursory thereof, or cells related thereto. The present invention was made by finding three species of novel genes by detecting genes specifically expressed in pancreases of PHHI...

20060057648 - Diagnostics and therapeutics for diseases associated with neuromedin u1 receptor (nmu1): The invention provides a human NMU1 which is associated with the hematological diseases, cardiovascular diseases, disorders of the peripheral and central nervous system, inflammation diseases, cancer diseases, and disorders of the liver. The invention also provides assays for the identification of compounds useful in the treatment or prevention of hematological...

20060057650 - Method for the immunocytological or molecular detection of disseminated tumor cells from a body fluid and kit that is suitable therefor: The invention relates to a method for enriching tumor cells from a body fluid, in which a cell separation medium of a specific density is overlaid with the body fluid and it is subsequently determined whether the enriched cells express an epithelial marker, for example a cytokeratin. A kit suitable...

20060057652 - Methods for identifying therapeutic agents and for treating disease: Included are methods of identifying compounds that mimic the interaction of Apoptin and APC1, e.g., binding of Apoptin to APC1, e.g., dissociation of APC1 from the APC/C, and compounds identified by the methods. Compounds that specifically mimic an interaction of Apoptin and APC1, e.g., cause dissociation of APC1 from the...

20060057651 - Polypeptides and antibodies derived from chronic lymphocytic leukemia cells and uses thereof: Small animal models for assessing immunomodulatory effects of compounds are provided....

20060057649 - Screening method and anti-tumor drug candidate obtained therefrom: A method for identifying a test compound that has an anti-tumoral effect whilst not significantly activating unrelated transduction signals, the method comprising the steps of: (a) selecting at least one test compound; (b) assaying the compound for anti-tumoral effect; (c) selecting at least one distinct intracellular event which is modulated,...

20060057653 - Detection of microorganisms with holographic sensors: A method for the detection of a cell comprises immobilizing the cell in a device also containing a sensor, and introducing a growth medium, wherein the sensor is sensitive to a product of the cell's growth; and detecting any change in an optical characteristic of the sensor. A device suitable...

20060057654 - Methods for determining susceptibility to dental caries: The present invention provides methods for determining whether a human being is susceptible to dental caries. The methods each include the steps of measuring the amount of α-defensins HNP 1, HNP 2 and HNP 3 in saliva obtained from a human being, and determining whether a reduced amount of the...

20060057647 - Detection and identification of saxiphilins using saxitoxin-biotin conjugates: A method for capturing a saxiphilin to allow for detection, characterisation, isolation an/or purification of said saxiphilin or its ligand, comprising: (1) providing a PST conjugate comprising a PST moiety bound via a linker through a site other than the binding site for saxiphilin directly or indirectly to a biotin...

20060057656 - Busulfan immunoassay: Novel conjugates of busulfan and novel busulfan immunogens derived from α-substituted derivatives of busulfan and antibodies generated by these busulfan linked immunogens are useful in immunoassays for the quantification and monitoring of busulfan in biological fluids....

20060057655 - Method for the detection of irradiation treatment of foods: An object of the present invention is to provide a novel method for detecting irradiation treatment of foods. The present invention provides a method for detecting irradiation treatment of foods comprising the steps of (A) obtaining a fraction containing one or more irradiation-generated fragments of natural high-molecular weight compound(s) from...

20060057657 - Stem cell selection and differentiation: Isolated mammalian stem cells sustainable in culture under glycolytic conditions and which maintain the potential to differentiate are provided. Further encompassed by the invention are functionally distinct subpopulations of stem cells with increased differentiation permissiveness....

20060057658 - Labeled substrate conjugates for identifying enzyme inhibitors: This invention provides labeled-substrate conjugates for assaying enzymes, particularly neuraminidases. Also provided are assays that are useful for identifying compounds that inhibit sialyltransferases or neuraminidases and may be useful in treating subjects with influenza. In particular, the present invention relates to methods of using such labeled substrate conjugates to screen...

20060057659 - Destructible surfactants and uses thereof: The present invention provides methods for enhancing chemical reactions of molecules, e.g., biomolecules, with destructible surfactants. The chemical reactions may involve and/or be associate with analysis, e.g., solubilizing, separating, purifying and/or characterizing the molecules. In one aspect, the anionic surfactants of the present invention may be selectively broken up at...

20060057660 - Method for measuring protein nmr: An object of the present invention is to provide a means for highly efficiently or quickly evaluating or determining the three-dimensional structure of a protein by conducting multidimensional multinuclear NMR measurement without performing a step of separating and purifying the synthesized labeled protein from other labeled proteins. The present invention...

20060057661 - Magnetic enzyme detection techniques: A diagnostic test kit for detecting the presence or quantity of an enzyme or enzyme inhibitor is provided. The diagnostic kit utilizes reactive complexes to facilitate the detection of the enzyme or enzyme inhibitor. The reactive complexes include a substrate joined (e.g., covalently bonded, physically adsorbed, etc.) to a reporter...

20060057668 - Antimicrobial polypeptide and utilization thereof: The present invention provides an artificially synthesized antimicrobial polypeptide that does not occur in nature and an antimicrobial agent comprising this polypeptide as the main component. This polypeptide contains in its peptide chain one or more types of nuclear localization signal sequences (NLSs) and/or one or more units of sequences...

20060057676 - Compositions and methods using the yeast ylr110c promoter: The invention provides novel yeast promoters useful for controlling the expression of homologous and heterologous nucleic acid molecules in yeast cells. The yeast promoters are induced by a fermentable carbon source, such as glucose, or a non-fermentable carbon source, such as ethanol, or both. Therefore, expression of nucleic acid molecules...

20060057673 - Epitope analogs: Some embodiments relate to analogs of peptides corresponding to class I MHC-restricted T cell epitopes and methods for their generation. These analogs can contain amino acid substitutions at residues that directly interact with MHC molecules, and can confer improved, modified or useful immunologic properties. Additionally classes of analogs, in which...

20060057672 - Exo-endo cellulase fusion protein: The present invention relates to a heterologous exo-endo cellulase fusion construct, which encodes a fusion protein having cellulolytic activity comprising a catalytic domain derived from a fungal exo-cellobiohydrolase and a catalytic domain derived from an endoglucanase. The invention also relates to vectors and fungal host cells comprising the heterologous exo-endo...

20060057665 - Galectin-14 therapeutic molecule and uses thereof: The present invention relates generally to a novel galectin and to derivatives, homologues, analogues, chemical equivalents and mimetics thereof capable of modulating an immune response and, in particularly, an inflammatory response. More particularly, the present invention relates to ecalectin-like galectin (herein referred to as “galectin-14”) and to derivatives, homologues, analogues,...

20060057670 - Genomic markers of hepatitis b virus associated with hepatocellular carcinomas: The present invention provides methods of predicting a pre-disposition of HBV-infected individuals to develop hepatacellular carcinoma (HCC)....

20060057666 - Human g protein coupled receptor: The invention provides isolated nucleic acids that encode GPCR-A-1, and fragments thereof, vectors for propagating and expressing GPCR-A-1 nucleic acids, host cells comprising the nucleic acids and vectors of the present invention, proteins, protein fragments, and protein fusions of the novel GPCR-A-1 isoforms, and antibodies thereto. The invention further provides...

20060057675 - Human toll homologue: The invention relates to the identification and isolation of novel DNAs encoding the human Toll protein PRO358 and its variants, and to methods and means for the recombinant production of these proteins. The invention also concerns antibodies specifically binding the PRO358....

20060057671 - Immobilized probes and methods of detecting conformationally altered prion proteins: The present invention provides methods of making immobilized probes that are specific for prion proteins, methods of using such probes to bind, detect, and remove prion proteins from samples, and kits for practicing the invention. The invention discloses immobilized probes that are locked into a particular, pre-determined configuration and that...

20060057669 - Inactive transcription factor tif-ia and uses thereof: Inactive transcription factor TIF-IA and uses thereof. Described are inactive forms of the human transcription initiation factor TIF-IA, preferably a mutant form of TIF-IA which lacks functionally important posttranslational modifications, e.g. phosphorylation, acetylation, glycosylation etc. Moreover, nucleic acid molecules encoding said TIF-IA are described as well as recombinant vectors containing...

20060057667 - Isolated human transporter proteins nucleic acid molecules encoding human transporter proteins and uses thereof: The present invention provides amino acid sequences of peptides that are encoded by genes within the human genome, the transporter peptides of the present invention. The present invention specifically provides isolated peptide and nucleic acid molecules, methods of identifying orthologs and paralogs of the transporter peptides, and methods of identifying...

20060057662 - Membrane scaffold proteins: Membrane proteins are difficult to express in recombinant form, purify, and characterize, at least in part due to their hydrophobic or partially hydrophobic properties. Membrane scaffold proteins (MSP) assemble with target membrane or other hydrophobic or partially hydrophobic proteins or membrane fragments to form soluble nanoscale particles which preserve their...

20060057663 - Novel g protein-coupled receptor protein and dna thereof: The present invention provides a human-derived protein or a salt thereof, a DNA encoding the same, a method for determination of ligand to the protein, a screening method/kits for a compound or its salt that alters a binding property between ligand and the protein, and the like. The human-derived protein...

20060057677 - Organic anion transporter and gene coding for the same: A protein capable of transporting organic anions having amino acid sequences represented by SEQ ID NO: 1 or 2 or amino acid sequences derived therefrom by deletion, substitution or addition of one or more amino acid residues; and a gene coding for the protein. The protein and gene therefor are...

20060057664 - Polypeptides and polynucleotides encoding same: The invention provides polypeptides, designated herein as SECP polypeptides, as well as polynucleotides encoding SECP polypeptides, and antibodies that immunospecifically-bind to SECP polypeptide or polynucleotide, or derivatives, variants, mutants, or fragments thereof. The invention additionally provides methods in which the SECP polypeptide, polynucleotide, and antibody are used in the detection,...

20060057674 - Translocating enzyme as a selection marker: The subject of the present invention is a selection system for microorganisms, which is based on the inactivation of an essential translocating enzyme and the curing of this inactivation by means of an indentically acting factor which is made available to the cells concerned by means of a vector. One...

20060057680 - Mutant interleukin-15 polypeptides: Mutant IL-15 polypeptides and compositions including the polypeptides are described herein. In various embodiments, a mutant IL-15 polypeptide is joined to a heterologous polypeptide. Also described herein are uses of the mutant IL-15 polypeptides, e.g., in suppressing immune responses....

20060057678 - Chimeric proteins for diagnosis and treatment of diabetes: Novel chimeric fusion proteins comprising immunodominant epitopes of GAD and insulin are provided. Also provided are immunomodulatory methods for the use of such proteins for both the prevention and treatment of Type 1 diabetes mellitus. The chimeric fusion proteins of the invention are useful in predicting risk of onset of...

20060057679 - Rage protein derivatives: The present invention is drawn to fusion proteins comprising a Receptor for Advanced Glycation Endproducts (RAGE) and an immunoglobulin element. The invention also encompasses methods of treating a condition characterized by activation of an inflammatory cytokine cascade comprising administering such fusion proteins. The invention is also drawn to nucleic acids...

20060057681 - Method for enhancing the effciency of an enzymatic purification process: This invention relates to methods for enhancing the efficiency of biological processes that involve successive enzymatic reactions. More specifically, the invention relates to methods for enhancing the efficiency of an enzymatic purification process by increasing the temperature between successive enzymatic purification steps....

20060057683 - Method for plasmid preparation by conversion of open circular plasmid to supercoiled plasmid: In one embodiment of the invention, a method is provided for preparing plasmid from host cells which contain the plasmid, comprising: (a) providing a plasmid solution comprised of unligatable open circular plasmid; (b) reacting the unligatable open circular plasmid with one or more enzymes and appropriate nucleotide cofactor(s), such that...

20060057682 - Mutation of dna polymerases from archaeobacteria: The present invention relates to a variant archaeal DNA polymerase having a modified amino acid sequence of a wild-type amino acid sequence, the modified sequence being in the amino-terminal amino acids that comprise a uracil-binding pocket in the wild-type polymerase whereby the variant polymerase has reduced affinity for uracil than...

20060057684 - Mashing process: The present invention provides processes for production of wort and beer from grist mashed at high temperature....

20060057685 - Method for producing abnormal amino acids using a bacterium of the enterobacteriaceae family having all acetohydroxy acid synthases inactivated: A method for producing abnormal amino acids is provided, such as norleucine and norvaline, using a bacterium of Enterobacteriaceae family, particularly a bacterium belonging the genus Escherichia which has all acetohydroxy acid synthases (AHASes) inactivated....

20060057686 - L-glutamic acid-producing microorganism and a method for producing l-glutamic acid: A coryneform bacterium which has an L-glutamic acid-producing ability and grows at least at the same growth rate as a non-mutated strain or a wild-type strain and has intracellular α-ketoglutarate dehydrogenase activity which is less than half that of the non-mutated or wild-type strain, and is obtained by introducing a...

20060057687 - Changing the fine chemical content in organisms by genetically modifying the shikimate pathway: The present invention relates to a process for the production of fine chemicals, in particular vitamin E, vitamin K and/or ubiquinone by culturing organisms, in particular plants, whose shikimate pathway is genetically modified over that of the wild type, and to the transgenic organisms themselves....

20060057688 - Method for separating vitamin k2 from bacillus bacterium culture: Vitamin K2 is separated from a cultured product of a Bacillus bacterium such as Bacillus natto by insolubilizing vitamin K2 contained in the cultured product using a metal ion of bivalent or more and separating the insolubilized vitamin K2 from the cultured product....

20060057690 - Glycerol-3-phosphate o-acyltransferase promoter for gene expression in oleaginous yeast: The promoter region associated with the Yarrowia lipolytica glycerol-3-phosphate O-acyltransferase (gpat) gene has been found to be particularly effective for the expression of heterologous genes in oleaginous yeast. The promoter regions of the instant invention have been shown to be suitable to drive high-level expression of genes involved in the...

20060057689 - Method for producing c4-c12 fatty acids: Processes for preparing fatty acids are described wherein a C4-C12 fatty acid methyl ester is subjected to hydrolysis in the presence of an enzyme to form an organic phase comprising a C4-C12 fatty acid and an aqueous phase comprising methanol, wherein at least a portion of the methanol is continuously...

20060057691 - Process for the extraction of polyhydroxyalkanoates from biomass: The present invention relates to a process for extracting polyhydroxyalkanoate from a biomass, comprising contacting the biomass with an organic solvent for a time, at a first temperature, and under a pressure that are sufficient to extract polyhydroxyalkanoate from the biomass to provide a composition comprising the organic solvent and...

20060057692 - Process for the extraction of polyhydroxyalkanoates from biomass: The invention relates to processes for extracting polyhydroxyalkanoate from a biomass, comprising admixing the biomass with an organic solvent for from 1 second to 15 minutes at a first temperature of from 5° C. below the melting point of the polyhydroxyalkanoate to 10° C. above the melting point of the...

20060057693 - Methods of treatment using electromagnetic field stimulated stem cells: Methods of modifying stem cells and compositions of modified stem cells, in particular mesenchymal stem cells, using electric or electromagnetic fields. In various embodiments, the present invention's methods are for the treatment of a human or other mammal subject in need thereof, comprising providing an in vitro culture comprising mesenchymal...

20060057695 - Ganp protein: The object of the present invention is to provide a novel protein having a kinase activity and a gene encoding said protein. According to the present invention, there is provided a GANP protein which is represented by the amino acid sequence shown in SEQ ID No. 1 or No. 3...

20060057694 - Rat receptor tyrosine knase, kdr: An isolated nucleic acid molecule encoding an optimized rat receptor type tyrosine kinase, KDR, is disclosed. The isolation of this KDR cDNA sequence results in disclosure of purified forms of rat KDR protein, recombinant vectors and recombinant hosts which express rat KDR....

20060057697 - Chondroitin systhetase and nucleic acid coding for the enzyme: A human-derived novel chondroitin synthase, which is an enzyme for synthesizing a fundamental backbone of chondroitin and has both glucuronic acid transferring activity and N-acetylgalactosamine transferring activity....

20060057698 - Nucleic acids and polypeptides involved in the production of cryptophycin: The present invention provides polypeptides involved in cryptophycin biosynthesis and the nucleic acid molecules that encode such polypeptides. The nucleic acid molecules and polypeptides of the invention or variants thereof can be used in the methods of the invention to produce cryptophycins....

20060057696 - Sugar chain synthases: The present invention provides O-glycan α2,8-sialyltransferase which has novel substrate specificity and substrate selectivity, and β-galactoside α2,6-sialyltransferase which has novel action and substrate specificity. The sialyltransferase of the present invention can be used as a medicament for suppression of cancer metastasis, prevention of virus infection, suppression of inflammatory response, or...

20060057701 - Antibodies directed against amyloid-beta peptide and methods using same: Monoclonal antibody 9TL and antibodies derived from 9TL directed against amyloid-beta peptide and methods of using same for diagnosing and treatment of Alzheimer's disease and Aβ peptide associated diseases are described. Methods of using antibodies directed against amyloid-beta peptide having impaired effector function for treatment of Alzheimer's disease and Aβ...

20060057702 - Antibodies directed against amyloid-beta peptide and methods using same: Monoclonal antibody 9TL and antibodies derived from 9TL directed against amyloid-beta peptide and methods of using same for diagnosing and treatment of Alzheimer's disease and Aβ peptide associated diseases are described. Methods of using antibodies directed against amyloid-beta peptide having impaired effector function for treatment of Alzheimer's disease and Aβ...

20060057700 - Compositions and methods for eliciting an immune response to gram-negative bacterial infections: Compositions for eliciting an immune response against Gram-negative bacterial infections and methods of making such compositions are provided. The composition comprises glycosylated pilin, the pilin being glycosylated with the O-antigen of a target Gram-negative bacteria of interest. Methods of eliciting an immune response by administration of such compositions are also...

20060057699 - Culturing anaplasma: The invention provides mammalian cells stably infected with Anaplasma species, as well as materials and methods related to propagating Anaplasma species in mammalian cells and isolating such Anaplasma species from mammalian cells....

20060057703 - Method for the evaluation of implantable materials: A method for the evaluation of a material to determine whether the material is susceptible to bacterial contamination or colonization comprising providing bacteria which are modified to produce a first detectable signal, exposing the material being evaluated to the bacteria and determining whether the first signal is present determining whether...

20060057704 - Composition: The present invention relates to a composition and a method of preparing or treating food products so as to improve their aroma, flavour, mildness, sweetness, consistency, texture, body, mouth feel, firmness, viscosity, gel fracture, wheying off, structure and/or organoleptic properties, nutrition and/or health benefits. In particular, the present invention provides...

20060057705 - Stimulation of microbial dechlorination of polychlorinated biphenyls with halogenated ethenes: Disclosed is a method for the anaerobic microbial dechlorination of polychlorinated biphenyls (PCBs). Polyhalogenated ethenes are used to stimulate the growth and dechlorinating activity of PCB dechlorinating bacteria in a contaminated soil or sediment. This method may be further coupled with aerobic microbial degradation of PCBs for the complete destruction...

20060057706 - Electroporation device: Electro-poration device for the permeabilization of cells contained in a substrate comprising a signal generator for generating a stimulating signal applied by means of electrodes to the substrate wherein an electric field permeabilizing the cells membranes is induced. The device calculates and monitors the instantaneous value or the ratio GT...

20060057707 - Optical detection of label-free biomolecular interactions using microreplicated plastic sensor elements: Methods and compositions are provided for detecting biomolecular interactions. The use of labels is not required and the methods can be performed in a high-throughput manner. The invention also provides optical devices useful as narrow band filters....

20060057709 - Plate and test method using the same: A flow path is formed in a zigzag shape to have a predetermined width from an upstream side to a downstream side. Accordingly, both side surfaces of the flow path divide the flow path into a plurality of regions from the upstream side to the downstream side to function as...

20060057708 - Testing plate: Light shielding portions are provided along a flow path on a top surface of a plate substrate at both sides, that is, an X1 side (right side) and an X2 side (left side) of the flow path. Then, when a fluorescent intensity is measured to determine whether a test sample...

20060057710 - Apparatus for monitoring bioluminescence of biological samples: An apparatus of monitoring the bioluminescence of organism samples comprises a culture part including a plurality of plates for putting plural organism samples and capable of cultivating said plural organism samples, a monitoring part for monitoring bioluminescences of said plural organism samples, a convey means for conveying said plural plates...

20060057711 - Air injected composter: The invention is an Air Injected Composter that accelerates the composting process by injecting air into the composting material, voiding the need for turning the composting matter for aeration. As the composting material is being aerated by air injected directly into the composting material, no other intervention is needed for...

20060057713 - Chamber for culturing cells and method for making same: Disclosed is a method for making an incubator, as well as an incubator structure. The disclosed method includes molding a chamber body. The chamber body defines an interior and an opening to the interior, and a door is provided to selectively seal the opening to the interior. During the molding...

20060057712 - Incubator and cell culturing method: In an apparatus including environment regulating means having a culture space 1h which can be sealed and serving to maintain an internal environment in the culture space 1h into a predetermined state, the environment regulating means of the apparatus includes temperature regulating means 10 for regulating a temperature in the...

20060057714 - Vector: The present invention provides a vector which can make nucleic acids to be an aggregate and locate a vector containing a cationic polymer to surround the aggregate so as to protect the nucleic acids from the enzyme. The vector comprises a polymer having branched chain(s). Preferably, 3, 4, or 6...

20060057715 - Methods and kits for isolating sperm cells: Disclosed are methods for isolating sperm cells from an aqueous sample and kits for isolating sperm cells from an aqueous sample....

20060057716 - Human malignant cystosarcoma phyllodes derived mouse cell line: A new mouse cell line, MCP-5, derived from human malignant cystosarcoma phyllodes was established. This invention provides methods for establishing MCP-5. MCP-5 may serve as both in vitro and in vivo models for studying pathogenesis and experimental therapy of human malignant cystosarcoma....

20060057719 - Carbohydrate determinant selection system for homologous recombination: This invention provides a system for selecting a cell that has undergone genetic alteration by homologous recombination from amongst a population of cells that do not have the alteration. The successfully targeted cells are identified and separated according to surface glycosylation that has changed as a result of the homologous...

20060057717 - Human bone accessory cells: Disclosed are compositions of bone accessory cells and methods for their preparation and use. Bone accessory cells are cells which are not hematopoietic and which can reconstitute the expression of bone proteins by human bone cells and support ex vivo expansion and/or differentiation of these cells. Such bone marrow-derived accessory...

20060057720 - Mesenchymal cells derived from human embryonic stem cells: This invention provides populations of mesenchymal cells obtained from pluripotent stem cells by differentiating them ex vivo. Multipotent mesenchymal cells can in turn be differentiated into more specialized cell types such as osteoblasts, with properties that make them suitable for reconstituting musculoskeletal cell function in an individual. The compositions, methods,...

20060057718 - Stem cell and progenitor cell expansion: The present invention provides compositions and methods for the expansion of stem cells and progenitor cells with adiponectin, adiponectin variants, or other molecules that activate adiponectin receptors and signaling, whereby the stem or progenitor cells retain their pluripotential phenotype after expansion. In certain embodiments, the present invention provides adiponectin-expanded stem...

20060057721 - Method of screening cell death modulators: The invention provides methods and compositions for identifying agents which modulate cell death, indicated e.g. by the expression of caspase-2 and/or caspase-7, in GDNF family growth factor deprived neuronal or nonneuronal cells. The methods for identifying such agents find particular application in drug development....

20060057722 - Conditioned medium of autologous or allogenic progenitor cells for angiogenesis treatment: A therapeutic composition is provided that comprises a cell-free conditioned medium containing mixed secretion products of isolated angiogenic progenitor cells obtained from bone marrow, peripheral blood, or adipose tissue. The composition may additionally contain angiogenesis-promoting proteins obtained by transfecting the progenitors cells in culture with an angiogenesis promoting transgene. The...

20060057724 - Nucleotide sequences and polypeptides encoded thereby useful for modifying plant characteristics and phenotypes: Isolated polynucleotides and polypeptides encoded thereby are described, together with the use of those products for making transgenic plants....

20060057723 - Transgenic plants containing molecular decoys that alter protein content therein: The present application describes isolated nucleic acids that contain a Nic gene product responsive element, and the use thereof in methods of producing transgenic tobacco plants having reduced levels of nicotine and/or TSNA therein, as well as other plants or host cells that contain altered levels of a protein of...

20060057725 - Therapeutic retroviral vectors for gene therapy: Retroviral gene therapy vectors that are optimized for erythroid specific expression and treatment of hemoglobinopathic conditions are disclosed....

20060057726 - Method for the positive selection of chromosomal mutations in c1 metabolizing bacteria via homologous recombination: A method for the positive selection of allelic exchange mutants is provided for C1 metabolizing bacteria. The chromosomal integration vectors, based on the pGP704 suicide vector, comprise at least one genetic selectable marker and the sacB gene, encoding levansucrase. A one- and two-step selection strategy is provided for the facile...

20060051732 - Ambient stored blood plasma expanders: A liquid plasma expander or resuscitation fluid composition for use in a subject in need thereof, comprising, consisting of, or consisting essentially of: (a) a keratin derivative (preferably alpha keratose, gamma keratose, or combinations thereof, and with basic alpha keratose preferred over acidic alpha keratose); and (b) an electrolyte solution,...

20060051731 - Processes for preparing lyophilized platelets: The present invention provides processes for preparing freeze-dried platelets, freeze-dried platelets made by those processes, platelets reconstituted from those freeze-dried platelets, and kits comprising those freeze-dried platelets. The freeze-dried platelets of the invention have similar characteristics to fresh platelets, have an exceptionally long shelf-life, and can be used for all...

20060051734 - Apparatus for mapping biological tissue quality: The present invention provides an apparatus suitable for use in investigating multi-phase biological tissue histology, which apparatus comprises a trans-ductally deployable probe mounting a periodically displaceable body of at least one tactile sensing device, said periodically displaceable body having an excitation frequency bandwidth in the range of from 1 Hz...

20060051738 - Dual glucose-turbidimetric analytical sensors: Diagnostic dry reagent tests capable of reacting with a single drop of whole blood and reporting both glucose and light-scattering analytes, such as chylomicrons, are taught. Such dry reagent tests may employ electrochemical detection methodologies, optical detection methodologies, or both methodologies. These tests alert diabetics to excessive levels of postprandial...

20060051735 - Method and devices for transferring biological cells between a carrier and a probe: A cell transfer method is described, in which transfer of at least one biological cell (21) describing a natural cell locomotion between a carrier (80) and a probe (10) is provided, and whereby positioning the probe (10) and the carrier (80) relative to one another in a transfer arrangement is...

20060051733 - Monitoring of cells: A method for monitoring cells in a microfluidic-device, wherein the device includes a chamber comprising a sensor, and the monitoring is under conditions such that attachment of cells to the surface of the chamber is inhibited....

20060051737 - Nucleic and amino acid sequences of human rif1 and methods of use thereof: The present invention relates to human Rif1 (hRif1) nucleic acid sequences and human Rif1 amino acid sequences encoded therefrom. The invention also encompasses antibodies that are immunologically specific for human Rif1 polypeptides. Also included in the present invention are methods directed to detecting Rif1 foci formed in response to various...

20060051736 - Paraffin-control marker: A sample configured to be cut to form a set of serial sections. The sample includes a sample block; at least one tissue sample substantially embedded in the sample block; and a least one control-marker core substantially embedded in the sample block and having a select shape as viewed from...

20060051744 - Feline infectious peritonitis (fip) and systemic multi-organ coronavirus biomarkers and screening methods: Methods for screening for FIP infection or other multi-organ coronaviruses are disclosed, as well as isolated antibodies and kits useful for performing such methods. Biomarkers for multi-organ coronavirus infections include soluble enolase; antibodies to enolase; and circulating immune complexes that contain enolase. The methods find application in diagnosis, treatment, vaccine-development,...

20060051745 - Hcv non-structural protein mutants and uses thereof: Modified HCV non-structural proteins are described. The proteins include modified NS3 domains such that proteolytic activity of the NS3 molecule is inhibited. The modified proteins retain conformational epitopes. HCV immunoassays including the modified NS3 molecules are also described....

20060051743 - Hepatitis b viral variants with reduced susceptibility to nucleoside analogs and uses thereof: The present invention relates generally to viral variants exhibiting reduced sensitivity to particular agents and/or reduced interactivity with immunological reagents. More particularly, the present invention is directed to hepatitis B virus (HBV) variants exhibiting complete or partial resistance to nucleoside or nucleotide analogs and/or reduced interactivity with antibodies to viral...

20060051740 - Method for the amplification and detection of hbv dna using a transcription based amplification: The present invention provides a method for the transcription based amplification of a target HBV nucleic acid sequence starting from HBV DNA optionally present in a sample, comprising the steps of,—incubating the sample, suspected to contain HBV, in an amplification buffer with one or more restriction enzymes capable of cleaving...

20060051739 - Methods and composition for visualizing and interfering with chromosomal tethering of extrachromosomal molecules: The invention provides methods and compositions for visualizing and interfering with chromosomal tethering and segregation of extrachromosomal molecules....

20060051742 - Novel assay for the separation and quantification of hemagglutinin antigens: The present invention relates to novel methods for separating hemagglutinin (HA) antigens, comprising the steps of applying a reduced and derivatized antigen preparation comprising solubilized HA antigens and a detergent in a pH controlled solution, on a Reversed-Phase High-Performance Liquid Chromatography column; and eluting the HA antigens from the column...

20060051746 - Peptides for inducing cytotoxic t lymphocyte responses to hepatitis b virus: Peptides are used to define epitopes that stimulate HLA-restricted cytotoxic T lymphocyte activity against hepatitis B virus antigens. The peptides are derived from regions of HBV polymerase, and are particularly useful in treating or preventing HBV infection, including methods for stimulating the immune response of chronically infected individuals to respond...

20060051741 - Plate for mass spectrometry, process for preparing the same and use thereof: The present invention provides a plate for mass spectrometry comprising a support and a PVDF-containing coating (that is, a PVDF-deposited thin layer) thereon, and a method of preparing a plate for mass spectrometry, which comprises coating the support surface with PVDF. The present invention also provides a method of analyte...

20060051747 - Production of vaccines: Means and methods for producing mammalian viruses, the method comprising infecting a culture of immortalized human cells with a virus, incubating the culture infected with virus to propagate the virus under conditions that permit growth of the virus, and to form a virus-containing medium, and removing the virus-containing medium. The...

20060051802 - Artificial receptors, building blocks, and methods: The present invention relates to artificial receptors, arrays or microarrays of artificial receptors or candidate artificial receptors, methods of and compositions for making them, methods of using them, and systems including them. The artificial receptor includes a plurality of building block compounds....

20060051798 - Bio-barcodes based on oligonucleotide-modified particles: The present invention relates to a screening methods, compositions, and kits for detecting for the presence or absence of one or more target analytes, e.g. proteins such as antibodies, in a sample. In particular, the present invention relates to a method that utilizes reporter oligonucleotides as biochemical barcodes for detecting...

20060051766 - Canine cyp1a2 genetic polymorphism: Disclosed is a method for determining whether a dog is an extensive metabolizer or a poor metabolizer in the rate of drug metabolism, by preparing a DNA sample from a dog, and determining a base corresponding to a base at position 1117 of a canine CYP1A2 gene (i.e., at position...

20060051758 - Chiral indole intermediates and their fluorescent cyanine dyes containing functional groups: This invention relates to the functionalized cyanine dyes and more particularly, to the synthesis of chiral 3-substituted 2,3′-dymethyl-3H-indole and its derivatives as intermediates for preparation of cyanine dyes, to methods of preparing these dyes and the dyes so prepared....

20060051759 - Compositions and methods relating to breast specific genes and proteins: The present invention relates to newly identified nucleic acid molecules and polypeptides present in normal and neoplastic breast cells, including fragments, variants and derivatives of the nucleic acids and polypeptides. The present invention also relates to antibodies to the polypeptides of the invention, as well as agonists and antagonists of...

20060051760 - Compositions and methods used for identifying factors required for the agglomeration of proteins: Described herein are compositions and methods for identifying cellular factors involved in protein agglomeration. One such factor is a nucleic acid component. Another factor is a cellular macromolecular co-binding component. The presence of certain NAs and co-binding factors facilitate the agglomeration of certain proteins. The compositions and methods described herein...

20060051767 - Cytochrome c acetylation: Modulation of cytochrome c acetylation, e.g., with a SIR polypeptide, enables interventions that modulate lifespan regulation and cell proliferation, e.g., by modulating apoptosis and/or mitochondrial function such as respiration....

20060051763 - Detection methods: The present invention relates to the prognosis, diagnosis and treatment of cancer, particularly prostate cancer. Polynucleotides having single nucleotide polymorphisms (SNPs) and haplotypes are provided which are of utility in the prognosis, diagnosis, prophylaxis and treatment of prostate and breast cancer....

20060051809 - Detection of nucleic acids by target-specific hybrid capture method: Target-specific hybrid capture (TSHC) provides a nucleic acid detection method that is not only rapid and sensitive, but is also highly specific and capable of discriminating highly homologous nucleic acid target sequences. The method produces DNA:RNA hybrids which can be detected by a variety of methods....

20060051756 - Device, method, and kit for gene detection: A gene detection tool comprising a sealable reaction chamber and a sealable detection chamber and a partition member positioned between the reaction chamber and the detection chamber that separates the reaction chamber and the detection chamber. The partition member is capable of unsealing; the reaction chamber is comprised of at...

20060051778 - Diagnosis of systemic lupus erythematosus: Diagnostic methods for the detection of SLE in a human body sample are disclosed. Nucleic acid hybridization and antibody-based methods derived from identification of Mycoplasma haemosapiens or its 16S sequence are described....

20060051757 - Diagnostic and therapeutic use of ensadin-0477 gene and protein for neurodegenerative diseases: The present invention discloses the differential expression of a gene coding for Ensadin-0477 in specific brain regions of Alzheimer's disease patients. Based on this finding, this invention provides a method for diagnosing or prognosticating a neurodegenerative disease, in particular Alzheimer's disease, in a subject, or for determining whether a subject...

20060051782 - Directed evolution of recombinant monooxygenase nucleic acids and related polypeptides and methods of use: The present invention relates to novel monooxygenase nucleic acids and polypeptides created using mutagenesis, DNA shuffling, or both, in a single iteration or multiple iterations, and methods for their creation and use. The monooxygenase enzymes of the present disclosure have particular utility as biocatalysts in industrial chemical redox reactions, such...

20060051765 - Dna hybrids and environment cleaning system employing dna hybrids: A DNA hybrid which comprises a porous oxide matrix and DNA immobilized thereon, and is useful for environmental clean-up, where the hybrid is prepared by removing a dispersion medium from a dispersion of colloidal oxide and DNA....

20060051768 - Dna markers for management of cancer: A method is provided for assessing allelic losses and hypermethylation of genes in CpG tumor promotor region on specific chromosomal regions in cancer patients, including melanoma, neuroblastoma breast, colorectal, and prostate cancer patients. The method relies on the evidence that free DNA and hypermethylation of genes in CpG tumor promotor...

20060051762 - Fixation carrier and solid phase: The object of the present invention is to provide an immobilization support having an optimum support surface for immobilizing various types of materials having different properties with specificity, efficiency, and good reproducibility, and to provide a solid phase having improved characteristics by use of the above support. The immobilization support...

20060051776 - Gene manipulation method using homologous recombination: Provided is a method of inserting an insert polynucleotide into a target nucleic acid having a first end and a second end by homologous recombination....

20060051786 - Genes and pathways differentially expressed in bipolar disorder and/or major depressive disorder: The present invention provides methods for diagnosing mental disorders. The invention also provides methods of identifying modulators of mental disorders as well as methods of using these modulators to treat patients suffering from mental disorders....

20060051803 - Genes useful for diagnosing and monitoring inflammation related disorders: Described herein is a system for monitoring gene expression for diagnosing and monitoring inflammation disorders, and for monitoring gene expression in inflammation disorders in response to a particular drug treatment reginmen. This system for detecting nucleic acid expression in a body fluid uses an isolated polynucleotide to detect expression of...

20060051790 - Genetic risk factor for neurodegenerative disease: The present invention relates to single base polymorphisms in the glycogen synthase kinase 3 beta and risk for developing neurodegenerative disease....

20060051810 - Kit for diagnosing gh deficiency: A kit for diagnosis of autosomal dominantly inherited isolated growth hormone (GH) deficiency (IGHD II) in a patient sample comprises means for analyzing a patient sample for the presence or absence of a T at nucleotide position 6191 in exon 4 of the GH-1 gene....

20060051751 - Major virulence factor detection and verocytontoxin type 2 subtype from clinical e. coli isolates using a one-step multiplex pcr: A single kit comprising 3 multiplex PCR assays that can detect in E. coli the presence of the 8 virulence genes: eaeA, EHEC-HlyA, Stx1 (VT1), Stx2 (VT2), Stx2c (VT2c), Stx2d (VT2d), Stx2e (VT2e) and Stx2f (VT2f) is described. In addition, the kit can detect the two critical serotypes (O157 and...

20060051805 - Method for detecting binding of nucleic acid and nucleic acid binding protein by monomolecular fluorescent analysis: A method for detecting binding of a nucleic acid and a nucleic acid binding protein by monomolecular fluorescent analysis....

20060051793 - Method for determining protein binding sites: A method for detecting a gene-noncoding region on a genomic DNA to which protein binds. Using fluorescent intensity data obtained with a conventional DNA chip, and based on positional information about the genome and intergenic sequence information, for example, protein binding sites are displayed. A cis element is detected from...

20060051750 - Method for fixing biomolecule on carrier: A nucleic acid is immobilized on a carrier by spotting a solution of the nucleic acid on the carrier comprising a synthetic resin such as polycarbonate, polymethyl methacrylate, acrylonitrile/butadiene/styrene copolymer, polyethylene, polyethylene terephthalate, polyphenol, polystyrene, polyacrylonitrile, polyvinyl chloride, or aramide, drying the solution, and irradiating the carrier with an ultraviolet...

20060051799 - Method for separating and purifying nucleic acid: Nucleic acid contained in a sample is highly efficiently recovered at a high recovery ratio by a method for separating and purifying nucleic acid using whole blood as the sample, which is a method for separating and purifying nucleic acid, comprising: preparing a sample solution containing nucleic acid; putting the...

20060051773 - Method for the mutagenesis of nucleotide sequences in plants, algae or fungi: The invention relates to a method for the mutagenesis of eukaryotic nucleotide sequences, preferably from plants, algae and/or fungi and a method for the production of genetically modified eukaryotic cells. The subject of the invention is the genetically altered nucleotide sequences of the type disclosed, vectors containing such nucleotide sequences,...

20060051771 - Methods and compositions for tailing and amplifying rna: The present invention provides methods and compositions that enable tagging and amplification of targeted RNA molecules. A targeted RNA molecule is any non-polyadenylated RNA molecule including, for example, miRNA, siRNA, rRNA, tRNA, synthetic RNA, or non-polyadenylated mRNA such as mRNA from bacteria. In certain aspects, the invention provides methods and...

20060051794 - Methods and compositions relating to col2a1 gene mutations and osteonecrosis: Methods, compounds, and kits for the diagnosis or screening of osteonecrosis are described, and the development of animal models for COL2A1 function in osteonecrosis is put forth. Novel mutations in the COL2A1 gene are identified that are associated with avascular necrosis of the femoral head. Methods of treatment of osteonecrosis...

20060051753 - Methods for determining the response of cells to vegf and uses thereof: The present invention provides methods of monitoring the progression of a disease condition associated with angiogenesis or vassculogenesis in a human subject in which a quantitative determination of the transcript level of at least one gene shown in Table 1 (by which is meant one or more of any of...

20060051755 - Methods of assaying for cell cycle modulators: The present invention relates to regulation of cellular proliferation. More particularly, the present invention is directed to nucleic acids encoding BRCA-1-Associated Protein-1 (BAP-1), Nuclear Protein 95 (NP95), Fanconi anemia group A protein (FANCA), DEAD/H box polypeptide 9 (DDX9), insulin-like growth factor 1 receptor (IGF1R), ubiquitin-conjugating enzyme E2 variant 1 (UBE2V1),...

20060051769 - Methods of genetic analysis of e. coli: The invention provides nucleic acid sequences which are complementary, in one embodiment, to a wide variety of E. coli genes. The invention provides the sequences in such a way as to make them available for a variety of analyses. In one embodiment the nucleic acid sequences provided are present as...

20060051770 - Methods of genetic analysis of yeast: Arrays of oligonucleotide probes which are complementary to a plurality of S. cerevisisae and S. pombe genes are disclosed. The arrays may be used to measure the expression levels of a plurality of genes simultaneously....

20060051791 - Methods of labelling polynucleotides with dibenzorhodamine dyes: including nitrogen- and aryl-substituted forms thereof, wherein substituents at positions C-14 to C18 taken separately are selected from the group consisting of hydrogen, chlorine, fluorine, lower alkyl, carboxylic acid, sulfonic acid, —CH2OH, alkoxy, phenoxy, linking group, and substituted forms thereof. The invention further includes energy transfer dyes comprising the dibenzorhodamine...

20060051789 - Methods of preparation of gene-specific oligonucleotide libraries and uses thereof: Methods of preparing gene-specific oligonucleotide libraries are disclosed. In one embodiment a double-stranded RNA corresponding to both sense and antisense strands of mRNA is digested by ribonuclease to produce short RNA fragments. In subsequent ligation steps, flanking oligoribonucleotides of defined sequences may be attached to the 3- and 5-ends of...

20060051748 - Multi-site mutagenesis: The present invention provides compositions and improved methods for multi-site directed mutagenesis and DNA shuffling. The present compositions and methods provide increased mutation frequency and increased number of transformants which allow one to sequence only a few clones in order to identify the correct mutants and to obtain the desired...

20060051806 - Mutations associated with iron disorders: The invention features a method of diagnosing an iron disorder, e.g., hemochromatosis, or a genetic susceptibility to developing such a disorder in a mammal by determining the presence of a mutation in exon 2 or in an intron of an HFE nucleic acid....

20060051775 - Non-invasive method for isolation and detection of fetal dna: A method of detecting the presence or absence of the fetal DNA sequence of interest in fetal DNA derived from a sample of peripheral blood obtained from a pregnant woman is described. The method involves obtaining a sample peripheral blood from a pregnant woman, treating the sample of peripheral blood...

20060051808 - Novel human dickkopf-related protein and nucleic acid molecules and uses therefor: Novel Dkk and Dkk-related polypeptides, proteins, and nucleic acid molecules are disclosed. In addition to isolated, full-length Dkk and Dkk-related proteins, the invention further provides isolated fusion proteins, antigenic peptides and antibodies. The invention also provides Dkk and Dkk-related nucleic acid molecules, recombinant expression vectors containing a nucleic acid molecule...

20060051774 - Novel nucleotide and amino acid sequences, and assays and methods of use thereof for diagnosis of prostate cancer: Novel markers for prostate cancer that are both sensitive and accurate. Furthermore, these markers are able to distinguish between prostate cancer and benign prostate hyperplasia (“BPH”). These markers are overexpressed in prostate cancer specifically, as opposed to normal prostate tissue and/or BPH. The measurement of these markers, alone or in...

20060051797 - Novel testis-specific genes in avian species: The present invention relates to a nucleotide sequence of a testis-specific gene in avian species and a method for identifying a testicular cell of avian species....

20060051781 - Nucleic acid encoding ds-cam proteins and products related thereto: In accordance with the present invention, there are provided novel Down Syndrome-Cell Adhesion Molecule (DS-CAM) proteins. Nucleic acid sequences encoding such proteins and assays employing same are also disclosed. The invention DS-CAM proteins can be employed in a variety of ways, for example, for the production of anti-DS-CAM antibodies thereto,...

20060051795 - Oligonucleotide mediated nucleic acid recombination: Methods of recombining nucleic acids, including homologous nucleic acids, are provided. Families of gene shuffling oligonucleotides and their use in recombination procedures, as well as polymerase and ligase mediated recombination methods are also provided....

20060051804 - Oligonucleotides labeled with stable isotopes and a method for detecting the same: Antisense oligonucleotide sequences which enable the measurement of the distribution and structure of antisense oligonucleotide drugs in the body, with lapse of time, and a method of detecting these sequences are provided. The antisense chains have a natural or non-natural nucleotide or peptide nucleic acid as a structural unit in...

20060051752 - One-step multiplex pcr for the identifiation and differentiation of campylobacter species: Described herein are a plurality of primers which may be used in a multiplex PCR assay in a fast, accurate, reliable and specific fashion for detecting the presence of specific Campylobacter strains within a sample. These kits can be used on bacterial isolates and has the potential for use directly...

20060051761 - Organic ultrathin film, cumulate thereof and process for producing the same: A novel organic ultrathin membrane characterized in that an amphiphilic compound having a pigment group and a nucleic acid base is aligned due to base-pairing with an oligonucleotide which is capable of forming a base pair with the nucleic acid base to thereby constitute a monolayer is provided. In this...

20060051800 - Polymeric beads for oligomer synthesis: The present invention provides solid support media for use in oligomer synthesis, methods of producing the media, and methods of using the media. In some embodiments, the processes of the invention comprise (a) providing an organic phase comprising an olefin monomer, a cross-linker, a functionalizing reagent and an initiator; and...

20060051780 - Polymorphic plasminogen genes and uses thereof: The present invention relates to polymorphic Plasminogen genes and polypeptides. In particular, the present invention provides assays for the detection of Plasminogen polymorphisms and mutations associated with disease states and provides screening assays for the identification and use of compounds that alter Plasminogen activity and/or biological pathways involving Plasminogen....

20060051749 - Polymorphism and haplotype scoring by differential amplification of polymorphisms: The current invention provides a method of performing DNA polymorphism assays. The assay combines allele-specific PCR with technology used for quantitative PCR. The method can be used to score the presence of absence of particular polymorphisms in a DNA sample. In a further aspect of the invention, the method is...

20060051788 - Probe set and substrate for detecting nucleic acid: Provided is a probe set having the same level of hybrid stability when a plurality of probes are assigned to an identical nucleic acid to be detected. The probe set is adjusted in the binding strength, for example a Tm value, of each probe in consideration of the nucleotide length...

20060051787 - Quantitative pcr for analysis of dna damage induced by pghs-2 (cox-2) or other factors: A method to assess DNA damage using quantitative polymerase chain reaction (QPCR) with an internal control with or without modified QPCR primers is disclosed. The method further included quantitation of QPCR products using a chemiluminescent, colorimetric or fluorescent assay and a real-time PCR. The chemiluminescent, colorimetric or fluorescent assay is...

20060051807 - Rapid parallel nucleic acid analysis: This invention provides methods for massive parallel nucleic acid analysis. A closed complex of nucleic acid template, nucleotide and polymerase can be formed during polymerase reaction, absent divalent metal ion. This is used to trap the nucleotide complementary to the next template nucleotide in the closed complex. Detection of the...

20060051796 - Real time pcr with the addition of pyrophosphatase: The present invention is directed to a method and a kit for amplifying and detecting a target nucleic acid, wherein the composition containing reagents to perform and monitor nucleic acid amplification in real time comprises at least a first hybridization probe labeled with a first fluorescent entity and a pyrophosphatase....

20060051801 - Representational fragment amplification: Methods and kits are provided for non-preferential amplification of a population of nucleic acids. The methods allow for a high degree of amplification, where representation in the final population has a direct linear relationship with the starting material. The amplification product is useful as a probe for hybridization; for generation...

20060051777 - Secreted and transmembrane polypeptides and nucleic acids encoding the same: The present invention is directed to novel polypeptides and to nucleic acid molecules encoding those polypeptides. Also provided herein are vectors and host cells comprising those nucleic acid sequences, chimeric polypeptide molecules comprising the polypeptides of the present invention fused to heterologous polypeptide sequences, antibodies which bind to the polypeptides...

20060051779 - Secreted and transmembrane polypeptides and nucleic acids encoding the same: The present invention is directed to novel polypeptides and to nucleic acid molecules encoding those polypeptides. Also provided herein are vectors and host cells comprising those nucleic acid sequences, chimeric polypeptide molecules comprising the polypeptides of the present invention fused to heterologous polypeptide sequences, antibodies which bind to the polypeptides...

20060051783 - Secreted and transmembrane polypeptides and nucleic acids encoding the same: The present invention is directed to novel polypeptides and to nucleic acid molecules encoding those polypeptides. Also provided herein are vectors and host cells comprising those nucleic acid sequences, chimeric polypeptide molecules comprising the polypeptides of the present invention fused to heterologous polypeptide sequences, antibodies which bind to the polypeptides...

20060051784 - Secreted and transmembrane polypeptides and nucleic acids encoding the same: The present invention is directed to novel polypeptides and to nucleic acid molecules encoding those polypeptides. Also provided herein are vectors and host cells comprising those nucleic acid sequences, chimeric polypeptide molecules comprising the polypeptides of the present invention fused to heterologous polypeptide sequences, antibodies which bind to the polypeptides...

20060051785 - Secreted and transmembrane polypeptides and nucleic acids encoding the same: The present invention is directed to novel polypeptides and to nucleic acid molecules encoding those polypeptides. Also provided herein are vectors and host cells comprising those nucleic acid sequences, chimeric polypeptide molecules comprising the polypeptides of the present invention fused to heterologous polypeptide sequences, antibodies which bind to the polypeptides...

20060051792 - Secreted and transmembrane polypeptides and nucleic acids encoding the same: The present invention is directed to novel polypeptides and to nucleic acid molecules encoding those polypeptides. Also provided herein are vectors and host cells comprising those nucleic acid sequences, chimeric polypeptide molecules comprising the polypeptides of the present invention fused to heterologous polypeptide sequences, antibodies which bind to the polypeptides...

20060051772 - Ssx-4 peptides presented by hla class ii molecules: The invention describes HLA class II binding peptides encoded by the SSX-4 tumor associated gene, as well as nucleic acids encoding such peptides and antibodies relating thereto. The peptides stimulate the activity and proliferation of CD4+ T lymphocytes. Methods and products also are provided for diagnosing and treating conditions characterized...

20060051764 - Thymidylate synthase polymorphisms for use in screening for cancer susceptibility: The present invention discloses a novel single nucleotide polymorphism (SNP) in the isolated 5′ tandem repeats of the thymidylate synthase (TS) gene and methods for its use. The novel SNP, located in the 12th nucleotide of a 28 bp third tandem repeat (3R) of the TS gene, substitutes a C...

20060051754 - Transporter selectively transporting sulfate conjugate and its gene: It is intended to provide a liver-specific organic anion transporter which transports selectively a sulfate conjugate and its gene. A protein capable of selectively transporting a sulfate conjugate; a gene encoding the same, more specifically, a gene having the amino acid sequence represented by SEQ ID NO:1 in Sequence Listing...

20060051813 - Diagnostic method for a transmissible spongiform encephalopathy or prion disease: A method is provided for the diagnosis of a transmissible spongiform encephalopathy (TSE) or prion disease in an animal which comprises assaying a sample obtained from said animal to determine the number of hematopoietic cells of the erythroid, megakaryocyte or platelet cell lineages in the sample or an expression product...

20060051814 - Methods for predicting outcome in traumatic brain injury: The invention describes methods for predicting outcome for patients suffering from traumatic brain injury (TBI) by evaluating levels of markers commonly associated with cellular damage in bodily fluids. Utilization of such methods improves diagnosis and treatment of patients suffering from traumatic brain injury, thus potentially minimizing and/or eliminating long-term adverse...

20060051812 - Novel carbohydrate compositions and a process of preparing same: The present invention is directed to novel methods to produce carbohydrate polymers and oligomers especially for pharmaceutical and food industries. The invention is directed to methods to remodel monosaccharides, and/or oligosaccharides and/or polysaccharides by a different monosaccharide, oligosaccharide or polysaccharide and optionally by further alcohol substances, under condensing conditions, preferably...

20060051811 - Site-specific labelling of proteins using acridone and quinacridone lifetime dyes: wherein B is an affinity tag; and L1 and L2 each independently comprise a group containing from 1 to 40 linked atoms selected from carbon atoms which may optionally include one or more groups selected from —NR′—, —O—, —CH═CH—, —CO—NH— and phenylenyl groups, where R′ is selected from hydrogen and...

20060051815 - Methods of treating smooth muscle cell disorders: The present invention provides methods of detecting cells showing smooth muscle differentiation. The present invention further provides methods of detecting tumor cells. The present invention further provides compositions and methods for treating smooth muscle cell disorders....

20060051819 - Detection, evaluation and treatment for advanced prostate cancer: The present invention includes novel compositions, methods and systems for detecting, evaluating and inhibiting the proliferation of prostate cancer cells by detecting and inhibiting the expression and activity of the proteoglycan Perlecan....

20060051817 - Method for diagnosing neoplasms: Disclosed are uses of Gangliosides GM1 and/or asialo-GM1 substances simulating the carbohydrate portion of said gangliosides with regard to bonding to anti-GM1 antibodies and/or anti-ADM1 antibodies for producing agents which bind or block anti-GM1 antibodies and/or anti-AGM1 antibodies which bond to natural killer cells (NKC) or for blocking antigen-presenting cells...

20060051818 - Method for ngf assay for in vitro diagnosis of breast cancer and therapeutic use: The present invention concerns a method for the in vitro diagnosis of breast cancer which consists in determining the presence of NGF in a biological sample derived from a patient suspected of suffering from breast cancer. Said method may be used both in early diagnosis, screening, therapeutic follow-up and prognosis,...

20060051820 - Method of examing staphylococcus aureus: e

20060051821 - Methods and kits for use in the diagnosis and treatment of endotoxemia: The invention provides methods and kits for use in determining whether a patient may benefit from treatment with a toll-like receptor 4 (TLR4) antagonist, such as an antiendotoxin compound....

20060051822 - Methods for detecting bacillus thuringiensis cryet33 and cry et34 polypeptides: Disclosed are Bacillus thuringiensis strains comprising novel crystal proteins which exhibit insecticidal activity against coleopteran insects including red flour beetle larvae (Tribolium castaneum) and Japanese beetle larvae (Popillia japonica). Also disclosed are novel B. thuringiensis crystal toxin genes, designated cryET33 and cryET34, which encode the colepteran-toxic crystal proteins, CryET33 (29-kDa)...

20060051823 - Compositions and methods for detecting treponema pallidum: Methods for the specific and highly sensitive detection of Treponema pallidum infection comprising the use of specific antigenic proteins and peptides unique to Treponema pallidum are provided. In particular, detection assays based on recognition of acidic repeat protein are provided. The methods of the present invention are useful for detection...

20060051816 - Rare cell detection using flat-panel imager and chemiluminescent or radioisotopic tags: Disclosed is a system using a large area flat panel imager which is specifically adapted for rare cell detection methods. The system generally includes an imager having a sample receiving surface which can provide a digital or electronic image of a sample deposited on the surface. The system also includes...

20060051824 - Tetrahydrocannabinoid antigens and method of use: The present invention is directed to Δ8-THC and Δ9-THC compounds useful for the covalent attachment to immunogenic molecules to form antigens for the preparation of specific binding molecules to Δ9-Tetrahydrocannabinol, Δ9-Tetrahydrocannabinoids, Δ8-Tetrahydrocannabinol, and Δ8-Tetrahydrocannabinoids, and their derivatives and metabolites. The present invention is directed to the compounds, their method of...

20060051825 - Methods and compositions for measuring canine bnp and uses thereof: The present invention describes compositions and methods designed to determine the presence or amount of BNP or fragments thereof in a sample. In particular, the invention provides materials that may be configured to bind canine BNP in a sandwich assay format. The present invention provides, inter alia, assays designed to...

20060051826 - Method and device for the incorporating a compound in the pores of a porous material and uses thereof: The invention relates to a process and to a device for the incorporation of a compound in the pores of a porous material chosen from microporous and mesoporous materials obtained by the sol-gel process and to the uses of this process and of this device. The process comprises the evaporation...

20060051827 - Method for increasing luminescence assay sensitivity: A method for increasing the sensitivity of a bio-luminescent assay comprising carrying out the assay in the presence of an organic compound that reduces luminescence that is not dependent on the presence of an analyte by at least about 10 fold, and that reduces, maintains, or increases the luminescence that...

20060051828 - Diagnostic test for determining the concentration of transient proteolytic activity in composite biological media: A method is provided for determining in real time the course of thrombin activity in a sample of blood or plasma as it appears in and disappears from the simple which comprises adding a thrombin substrate to the sample that, per unit time, produces a detectable signal in a quantity...

20060051829 - Regulating pas domain function with foreign pas ligands: A functional surface binding specificity of a PAS domain, wherein the PAS domain is predetermined, prefolded in its native state, and comprises a hydrophobic core that has no NMR-apparent a priori formed ligand cavity, is changed by (a) introducing into the hydrophobic core of the PAS domain a foreign ligand...

20060051830 - Quantitation of enzyme activity using planar waveguides: Enzyme activity sensors comprising a planar waveguide can be used for sensitive and quantitative measurement of enzyme activity. Such enzyme activity sensors are useful in a variety of settings, including clinical, commercial, and public health settings....

20060051831 - Method and kit for quantitative analysis of protein: The present invention provides a method for global quantitative analysis of protein that is effectively applied also for unpurified samples such as biological samples, and achieves better detection sensitivity and quantitativeness than the conventional NBS method. A method for global quantitative analysis of protein comprising: preparing two states of protein...

20060051832 - Modified sarcosine oxidase, process for producing the same and reagent composition using the same: A protein having been modified by addition, deletion, insertion or substitution of at least one amino acid in an amino acid sequence constituting a protein having a sarcosine oxidase activity and still having the sarcosine oxidase activity, characterized by having an improved stability in the state of a liquid compared...

20060051833 - Method of isolating stem cells: The present invention relates, in general, to stem cells, and in particular, to a method of isolating stem cells and to reagents suitable for use in such a method. The invention further relates to stem cell populations isolatable in accordance with the present method....

20060051834 - Method for preparative in vitro protein biosynthesis: The invention relates to a method for preparative in vitro protein synthesis of an expression product in a cell-free transcription/translation system, comprising the following steps: a) in a reaction vessel, a reaction solution is prepared, comprising the following synthesis substances: components of the transcription/translation apparatus for a defined expression product,...

20060051839 - Dna expression vectors and methods of use: The present invention relates to novel plasmid constructs useful for the delivery of DNA vaccines. The present invention provides novel plasmids having a transcription cassette capable of directing the expression of a vaccine nucleic acid insert encoding immunogens derived from any pathogen, including fungi, bacteria and viruses. The present invention,...

20060051838 - Genetic computing using combinatorial transcription control: A scheme is provided for selecting the regulatory DNA sequences to exert combinatorial control of gene transcription in vivo by multiple regulatory proteins, i.e., transcription factors (TFs). The method provides the ability to implement a wide range of complex logic functions through the manipulation of the regulatory DNA sequences, typically...

20060051840 - Hybrid expression of neisserial proteins: Two or more Neisserial proteins (e.g. A and B) are expressed as a single hybrid protein which can be represented simply by the formula NH2-A-B—COOH....

20060051841 - Identification, cloning, expression, and purification of three novel human calcium-independent phospholipase a2 family members possessing triacylglycerol lipase and acylglycerol transacylase activities: Isolated novel and purified and characterized phospholipase A2, referred to herein as calcium-independent phospholipase A2zeta (iPLA2zeta) having SEQ. ID. NO: 2 (See FIG. 1), and nucleic acid sequence (SEQ. ID. NO: 4), and calcium-independent phospholipase A2eta (iPLA2eta) having SEQ. ID. NO: 3 (See FIG. 1), and nucleic acid sequences (SEQ....

20060051836 - Molecules for disease detection and treatment: Various embodiments of the invention provide human molecules for disease detection and treatment (MDDT) and polynucleotides which identify and encode MDDT. Embodiments of the invention also provide expression vectors, host cells, antibodies, agonists, and antagonists. Other embodiments provide methods for diagnosing, treating, or preventing disorders associated with aberrant expression of...

20060051843 - Novel fluorescent proteins: A GFP with an F64L mutation and an E222G mutation is provided. This GFP has a bigger Stokes shift compared to other GFPs making it very suitable for high throughput screening due to a better resolution. This GFP also has an excitation maximum between the yellow GFP and the cyan...

20060051842 - Nucleic acid encoding alpha chain of human il-11 receptor: The present invention relates generally to novel haemopoietin receptors, or components or parts thereof and to a method for cloning genetic sequences encoding same. More particularly, the subject invention is directed to recombinant or synthetic haemopoietin receptors or components or parts thereof. The receptor molecules or components or parts thereof...

20060051835 - Pael receptor, cells and animal expressing pael receptor and method of screning remedy for parkinson's disease: An object of the present invention is to provide a substrate of Parkin, a system for elucidating the cause of Parkinson's disease using the substrate and a method of screening for a remedy for Parkinson's disease. An animal cell having a DNA encoding a human Pael receptor or a DNA...

20060051837 - Polynucleotides and polypeptides coded by said polynucleotides involved in the synthesis of diketopiperazine derivatives: The invention concerns novel isolated natural or synthetic polynucleotides and polypeptides coded by said polynucleotides, involved in the synthesis of diketopiperazine derivatives, vectors comprising said polynucleotides, micro-organisms transformed with said polynucleotides, uses of said polynucleotides and said polypeptides, as well as methods for the synthesis of diketopiperazine derivatives, including cyclodipeptides...

20060051844 - Human epo mimetic hinge core mimetibodies, compositions, methods and uses: The present invention relates to at least one novel human EPO mimetic hinge core mimetibody or specified portion or variant, including isolated nucleic acids that encode at least one EPO mimetic hinge core mimetibody or specified portion or variant, EPO mimetic hinge core mimetibody or specified portion or variants, vectors,...

20060051845 - Process for purifying difructose dianhydride iii: In the invention, highly pure crystals of difructose dianhydride III (di-D-fructofuranose-1,2′:2,3 ′-dianhydride; hereinafter referred to as DFA III) are produced by adding 5% or less of powdered active carbon to a DFA III containing purified solution containing DFA III of 90% or more purity at a concentration of R-Bx 10-60,...

20060051846 - Process for producing l-alpha-methylcysteine derivative: A process for easily producing an L-α-methylcysteine derivative or its salt, which is useful as a drug intermediate, from a cheap easily procurable raw material through an enzymatic D-stereoselective hydrolysis of racemic 5-halomethyl-5-methyl-hydantoin. L-α-methylcysteine derivative or its salt is produced by converting racemic 5-halomethyl-5-methylhydantoin to L-5-halomethyl-5-methylhydantoin through an enzymatic D-stereoselective...

20060051847 - Metabolically engineered cells for the production of polyunsaturated fatty acids: The present invention relates to the construction and engineering of cells, more particularly microorganisms for producing PUFAs with four or more double bonds from non-fatty acid substrates through heterologous expression of an oxygen requiring pathway. The invention especially involves improvement of the PUFA content in the host organism through fermentation...

20060051848 - Novel ethanol producing bacterium and process for producing ethanol: A bacterium capable of producing ethanol at a temperature of 40° C. or more from a carbon compound which is gaseous at ordinary temperature and ordinary pressure as the raw material, and a process for producing ethanol by culturing the bacterium at 40° C. or more....

20060051849 - Thermostable alpha-amylases: An isolated polynucleotide comprising an open reading frame encoding a polypeptide having alpha-amylase activity, the polypeptide selected from the group consisting of: a) a polypeptide comprising an amino acid sequence which has at least 70% identity with amino acids 22 to 450 of SEQ ID NO:4; b) a polypeptide comprising...

20060051850 - Isolated human transporter proteins, nucleic acid molecules encoding human transporter proteins, and uses thereof: The present invention provides amino acid sequences of peptides that are encoded by genes within the human genome, the transporter peptides of the present invention. The present invention specifically provides isolated peptide and nucleic acid molecules, methods of identifying orthologs and paralogs of the transporter peptides, and methods of identifying...

20060051852 - Apyrase polypeptides and nucleic acids encoding same: The present invention provides novel isolated polynucleotides encoding a novel extracellular nucleotidase, which belngs to a new family of calcium dependent apyrases. Also provided are antibodies that immunospecifically bind to the apyrase polypeptide or any derivative (including fusion derivative), variant, mutant or fragment of the novel polypeptide, polynucleotide or antibody....

20060051851 - Novel selenocysteine-containing protein: A novel selenocysteine-containing protein is provided which is a substantial entity having an enzymatic activity, typically an activity to reduce peroxide phospholipids. There are provided a gene consisting of a coding sequence for a protein not containing selenocysteine in which the codon for selenocysteine is introduced at a desired position...

20060051853 - Human mekk proteins, corresponding nucleic acid molecules and uses therefor: Isolated nucleic acid molecules encoding human MEKK proteins, and isolated MEKK proteins, are provided. The invention further provides antisense nucleic acid molecules, recombinant expression vectors containing a nucleic acid molecule of the invention, host cells into which the expression vectors have been introduced and non-human transgenic animals carrying a human...

20060051854 - Method for the production of proteins: The present invention relates to a process for the purification of a protease....

20060051855 - Microbial materials for degradation of oils and toxic chemicals: Provided is microbial materials for degradation of oils and toxic chemicals. The microbial material includes a mixture comprising a microorganism and culture filtrate capable of degrading oil and toxic chemicals being at least one selected from the group consisting of Trichosporon loubieri Y1-A of deposit No. KCTC 18079P, Trichosporon cutaneum,...

20060051856 - Bioreactor system for biological degradation of oily sludge: A 10,000 gallon sequencing batch reactor tank for the on-site bio-degradation of oily sludge. Bacteria already present in and adapted to oily sludge degrade the hydrocarbons found in oily sludge within two weeks from 20,000 ppm to less than 100 ppm. A degradation cycle requires 5 days. After five days...

20060051857 - Cell culture insert: The invention relates to a cell culture insert (1) comprising a beaker-shaped insert wall (11), a base (9) consisting of a membrane filter, projecting support arms (3, 4) that are distributed around the periphery of the top and lateral spacers (7, 8) for a vertical and horizontal orientation in a...

20060051858 - Microreactor, method for preparing same, and method for producing a biochemical or biological reaction: Microreactor comprising an inlet or feed channel, an inlet or feed zone (1) for a flow of fluid, a reaction zone (2), an outlet zone (3) and an outlet or evacuation channel, said zones (1, 2, 3) and channels (46, 48) being in fluid communication, and at least one compound...

20060051859 - Long acting human interferon analogs: Compositions, kits and methods are provided for Interferon analogs in order to promote general health or for therapeutic treatment of diseases. Human interferon analogs are made by fusion of interferon with human serum albumin. The bio-assay shows that the interferon analogs with the same cell protection against viral attack have...

20060051861 - Cell lines for use in increasing protein yield from a cell culture: The present invention provides cell lines useful in the production proteins and peptides. The cell lines contain recombinant expression constructs. The recombinant expression construct encodes the STPs consisting of the Cy protein motif and/or an ankyrin-binding protein motif. Each recombinant expression construct also contains an inducible transcription regulation element having...

20060051860 - Method of organ regeneration: It is intended to provide a method of regenerating an organ or a part thereof which comprises transplanting bone marrow or hematopoietic stem cells into an injured mammal or a mammal having an injured in organ or a part thereof; a method of treating injury: a method of producing an...

20060051863 - Meiosis arrest in human oocytes in vitro: The method for in vitro synchronisation of nuclear and cytoplasmatic maturation of GV oocytes from domestic animals or from primates can be improved if a phosphodiesterase type 3 inhibitor is added to the medium after collection of the oocytes and, thereafter, said phosphodiesterase type 3 inhibitor is removed to allow...

20060051862 - Methods of preparing feeder cells-free, xeno-free human embryonic stem cells and stem cell cultures prepared using same: The present invention is of methods of establishing and propagating human embryonic stem cell lines using feeder cells-free, xeno-free culture systems and stem cells which are capable of being maintained in an undifferentiated, pluripotent and proliferative state in culture which is free of xeno contaminants and feeder cells....

20060051865 - Systems and methods for isolating stromal cells from adipose tissue and uses thereof: Methods of isolating cells from adipose tissue that have potential to differentiate into cells of mesenchymal origin, including cells of chondrogenic, osteogenic, adipogenic, and/or myogenic origin, comprising: (a) subjecting adipose tissue to an electromagnetic, sonic, or other wave energy source; and (b) centrifuging the tissue to form a pellet comprising...

20060051864 - Tissue culture medium for culturing potentially regenerative cells and functional tissue-organs in vitro: Compositions and methods are provided for culturing in vitro potentially regenerative cells (PRCs) from which functional tissue-organs are regenerated. In one aspect of the invention, a tissue culture medium is provided which comprises at least 50% of water and a sterol compound that is dissolved in a fatty acid-containing oil...

20060051866 - Means of producing and utilising a population of disease specific cytotoxic t-lymphocytes: The present invention relates to the production of a population of virus-specific CTLs in which the population contains an anti-virus specific gene expression construct. This population can be combined with a population of CD4+ T lymphocytes or a population of CD34+ hematopoietic progenitor cells and one or more of these...

20060051867 - Cell growth: A substrate for cell growth comprises a polysaccharide and a cell adhesion protein provided at the surface of the substrate. The polysaccharide may for example be an alginate and the cell adhesion protein may be fibronectin, vitronectin or von Willebrand protein. The substrate may for example be a fibre and...

20060051868 - Methods for increasing conifer somatic embryo initiation, capture, and multiplication: The present invention provides methods for initiating, capturing, maintaining and multiplying embryogenic cultures of coniferous plants. Methods include the use of novel media compositions containing folic acid, biotin, pH buffers, gibberellin inhibitors and gas-tight environment treatments to improve the frequency of embryogenic tissue initiation, capture, maintenance and multiplication. The methods...

20060051869 - Transport proteins and their uses: The present invention relates to transport proteins, in particular VP22 and homologues thereof, and to methods of delivering these proteins and any associated molecules to a target population of cells. This transport protein has applications in gene therapy and methods of targeting agents to cells where targeting at high efficiency...

20060051870 - Methods and compositions for the introduction of molecules into cells: The present invention is directed to the introduction of molecules, including nucleic acids, carbohydrates, plant growth regulators and peptides into cells and tissues. The present invention is also directed to media and methods for enhancing embryogenic callus production of elite lines of soybean....

20060046243 - Method for vitrification of mammalian cells: A method of vitrifying mammalian cells. According to the method of the present invention, biological cells of mammalian origin are frozen quickly by a vitrification method. Upon exposure to a coolant, the biological cells undergo vitrification. The biological cells which have undergone vitrification may be stored for a period of...

20060046244 - Automated seed sampler and methods of sampling, testing and bulking seeds: An automated seed sampler includes a sampling station; a sampler for removing material from a seed in the sampling station; a seed conveyer for conveying the seed from the sampling station to a compartment in a seed tray; and a conveyor for conveying the material removed from the seed to...

20060046245 - Porous device: A porous device for use in a method of synthesis comprising a body having an internal region which is porous wherein an active material, for example a solid support resin, is entrapped within the internal region....

20060046246 - Genus, group, species and/or strain specific 16s rdna sequences: Materials and methods for identifying unique sites in bacterial 16S and 23S rDNA are provided, as well as specific unique sequences of 16S rDNA in select bacteria. The distinguishing moieties will enable rapid differentiation between families, genera, groups, species, strains, subspecies, and isolates of microorganisms. Such differentiation can be performed...

20060046247 - Protecting avians from pathogens: The invention relates to nucleotide sequences which encode therapeutic polynucleotides which correspond to one or more certain sequences in the genome of an avian pathogen. The invention also relates to transgenic avians whose cells contain such nucleotide sequences....

20060046248 - Rna interference in avians: The invention relates to nucleotide sequences which encode therapeutic polynucleotides which correspond to one or more certain sequences in the genome of an avian pathogen. The invention also relates to transgenic avians whose cells contain such nucleotide sequences....

20060046260 - Amplified bioassay: A method for assaying a biological sample includes forming sensitized microcapsules filled with unique oligomarkers, capturing sensitized microcapsules in the presence of analytes, releasing oligomarkers from microcapsules and detecting and measuring oligomarkers to detect and quantify presence of analyte in biological sample. Using encapsulated oligomarkers provides for an amplified high...

20060046263 - Analysis: A method for processing results, particularly from, the method comprising: obtaining from the results information concerning the single nucleotide polymorphisms implied for one or more loci, the information including identity information on the single nucleotide polymorphism or polymorphisms of a locus and a value related to the level detected for...

20060046258 - Applications of single molecule sequencing: The invention provides methods for determining the presence of a disease by comparing a sequence from a single target molecule with a predetermined sequence that is associated with a specific disease....

20060046264 - Automated seed sampler and methods of sampling, testing and bulking seeds: An automated seed sampler includes a sampling station; a sampler for removing material from a seed in the sampling station; a seed conveyer for conveying the seed from the sampling station to a compartment in a seed tray; and a conveyor for conveying the material removed from the seed to...

20060046261 - Compositions and methods employing zwitterionic detergent combinations: The present invention provides lysis reagents, containers, methods and kits relating to the extraction or the extraction and isolation of a cellular component from a host cell. More specifically, the invention provides combinations of zwitterionic compounds that may be employed to aide in the extraction or the extraction and isolation...

20060046254 - Determination of nucleic acid using electrocatalytic intercalators: Compositions and methods for electrochemical detection of nucleic acid are disclosed. The compositions comprise electrocatalytic intercalators that bind to double-stranded nucleic acid and oxidize nucleic acid. Methods of detecting nucleic acid in a sample comprise forming double-stranded nucleic acid that is bound by electrocatalytic intercalators wherein the intercalators catalyze oxidation...

20060046259 - Differential expression of molecules associated with acute stroke: Methods are provided for evaluating a stroke, for example for determining whether a subject has had an ischemic stroke, determining the severity or likely neurological recovery of a subject who has had an ischemic stroke, and determining a treatment regimen for a subject who has had an ischemic stroke, as...

20060046251 - Element defined sequence complexity reduction: A method for providing defined mixtures of nucleic acids is described. In certain embodiments, the method uses oligonucleotide probes attached to a solid support as a sequence-specific affinity agent to isolate and facilitate the amplification of defined nucleic acid fragment mixtures....

20060046256 - Identification of informative genetic markers: The present teachings describe methods for selecting informative genetic markers including single nucleotide polymorphisms (SNPs) that may be used in the design and execution of genome wide association studies. These methods are distinguished from other methods relying on a predefined haplotype block structure and may be configured to make use...

20060046249 - Identification of polynucleotides and polypetide for predicting activity of compounds that interact with protein tyrosine kinase and or protein tyrosine kinase pathways: The present invention describes polynucleotides and polypeptides that have been discovered to correlate to the relative intrinsic sensitivity or resistance of cells, e.g., colon cell lines, to treatment with compounds that interact with and inhibit src tyrosine kinases. These polynucleotides and polypeptides have been shown, through a weighted voting cross...

20060046266 - Inkjet-type dna arrayer able to perform pcr and method of manufacturing dna microarrays using the same: An inkjet-type DNA arrayer for performing PCR and a method of manufacturing DNA microarrays using the same are provided. The inkjet-type DNA arrayer includes: a substrate having a plurality of flow paths filled with a PCR solution; a plurality of first heaters heating the PCR solution according to the PCR...

20060046252 - Method and system for developing probes for dye normalization of microarray signal-intensity data: A method and system for determining a set of dye-normalization microarray probes that consistently hybridize to approximately the same number of target molecules in a wide range of sample solutions. The method of one embodiment of the method of the present invention generates a set of candidate probe molecules. The...

20060046253 - Method for analyzing genes of industrial yeasts: m

20060046257 - Novel nucleotide and amino acid sequences, and assays and methods of use thereof for diagnosis of lung cancer: Novel markers for lung cancer that are both sensitive and accurate. These markers are overexpressed in lung cancer specifically, as opposed to normal lung tissue. The measurement of these markers, alone or in combination, in patient samples provides information that the diagnostician can correlate with a probable diagnosis of lung...

20060046250 - Novel use of aim 3 acting as a tumor suppressor: The present invention relates to novel uses of AIM3 acting as a tumor suppressor, and more particularly to methods for using an AIM3 protein or a nucleic acid encoding the protein to activate ATM or ATR and to treat ATM- or ATR-mediated diseases. The AIM3 protein according to the present...

20060046262 - Reusable substrate for dna microarray production: The invention relates to a method for producing biopolymer arrays comprising a porous membrane and bound biopolymers. In particular, said production of biopolymer arrays comprises electrochemical production cycles....

20060046265 - Single-primer nucleic acid amplification methods: The present invention is directed to novel methods of synthesizing multiple copies of a target nucleic acid sequence which are autocatalytic (i.e., able to cycle automatically without the need to modify reaction conditions such as temperature, pH, or ionic strength and using the product of one cycle in the next...

20060046255 - Use of nucleic acid analogues in diagnostics and analytical procedures: The present invention pertains to certain nucleic acid analogs and related kits that are useful for the capture, recognition, detection, identification, or quantification of certain chemical or biological entities....

20060046270 - Analytical method: The instant invention describes an analytical assay to accurately measure an analyte in the presence of an interfering substance....

20060046271 - Assessment of cancer susceptibility to molecular targeted therapy by use of recombinant peptides: A method for assessing cancer susceptibility to molecular targeted therapy. Also provided are methods for in vivo panning of diverse molecules for isolation of targeting ligands that specifically bind an apoptotic cell associated with a responding tumor, targeting ligands identified by the panning methods, and diagnostic and imaging uses therefor....

20060046268 - Diagnosis of anemia and optimizing treatment for heart repair: A method of diagnosis of anemia is disclosed whereby blood is withdrawn from a patient and the level of hematopoietic stem cells (HSC) in the blood are determined by detecting a cell surface protein chosen from ACE+, CD34+, CD38, CD90+ (thyl) and Lin−. Patients with low levels of HSC are...

20060046267 - Method for identification and determination of hypersensitivity of a patient to abacavir: A method for identifying whether a patient will exhibit a hypersensitive reaction or like-reaction to treatment with abacavir comprising the step of: typing the patient for the presence of the 57.1 ancestral haplotype....

20060046269 - Methods and devices for processing chemical arrays: The subject invention provides methods and devices for processing at least one chemical array on a flexible substrate. Also provided are methods of using chemical arrays produced according to the subject invention, and arrays processing according to the subject methods, and arrays processed according to the subject methods, as well...

20060046272 - Whole blood preparation for cytometric analysis of cell signaling pathways: This invention is directed to a method for preparation of a biological sample for measurement of protein epitopes that allows for the preservation of intracellular protein epitopes and detection of signal transduction pathways based on the ability to capture transient activation states of the epitopes. The method provided by the...

20060046276 - Acetyl-ldl receptor as a biomarker for breast cancer: The present invention is a biomarker for breast cancer, including early stage and precancerous conditions. Patients exhibiting reduced expression of acetyl-LDL receptor in a NAF sample collected from a breast that is below the 95% confidence limit of normals either have breast cancer are at high risk to develop breast...

20060046275 - Blood urea nitrogen (bun) sensor: A BUN (blood urea nitrogen) sensor containing immobilized carbonic anhydrase and immobilized urease for the in vitro detection of urea nitrogen in blood and biological samples with improved performance and precision characteristics....

20060046273 - Homogeneous enzyme immunoassay for oral fluid: The present invention discloses homogeneous enzyme immunoassay systems, methods and kits useful for the qualitatively and quantitatively determination of analytes in oral fluid samples. The system involves a competitive enzyme immunoassay employing a conjugate comprising glucose-6-phosphate dehydrogenase (G6PDH) and an analyte. The methods and kits are particularly useful in the...

20060046274 - Inhibin-a: a marker for differentiation, diagnosing and screening abnormal pregnancies: The present invention relates to a method, based on the hormone Inhibin-A, of diagnosing, screening and differentiating between normal pregnancies and abnormal pregnancies. The method can be used to differentiate between viable and non-viable pregnancies. Particularly, Inhibin-A can also be used in a method and kit for the diagnosis of...

20060046277 - Protein kinase and phosphatase substrates and multiplex assays for identifying their activities: The present invention provides protein kinase and protein phosphatase substrates, methods of detecting protein kinases and protein phosphatases, and kits for detection of protein kinases and protein phosphatases. The substrates, methods, and kits use multiple substrates that can easily be differentiated from all other substrates, thus enabling rapid and easy...

20060046278 - Methods and kits for determining metabolic stability of compounds: The present invention provides methods and kits for determining the metabolic stability of compounds (e.g., stability to oxidative metabolism). In particular embodiments, the invention provides a method of determining the susceptibility of a compound to metabolism by an enzyme. In one representative embodiment, the enzyme is a cytochrome P450 enzyme....

20060046279 - Analytical methods utilizing real-time energy/particle interaction-based determination techniques: Analytical methods utilizing energy/particle interaction assessment techniques, useful for monitoring and screening applications, including determinations of individuals suitable for inclusion in clinical trial test subjects, monitoring of the inception and progression of disease states, determinations of the character of drug/target interactions for drug discovery, determinations of best modes of therapeutic...

20060046280 - Method for determination of platelets quality: There is provided a method for assessing the quality of platelet concentrates for determining their suitability for transfusion. More specifically dynamic light scattering measurements of the samples containing platelets are obtained and parameters, such as hydrodynamic radius and relative number of platelet derived microparticles, are derived from these measurements that...

20060046281 - Non-destructive method and test kit for the detection of infestation of live trees by invasive wood boring insect species: The invention disclosed is a method and test kit for the non-destructive detection of infestation of live conifer trees by invasive wood boring insects having a specific established fungal associate. The method involves identifying an infested tree, providing a probe for collecting viable fungal spore samples and matching the spore...

20060046282 - Template methods and devices for preparing sample arrays: A method for preparing a microarray that includes placing at least one template over a first surface of the recipient block, wherein the template defines an array of openings and the recipient block has a plurality of receptacle holes, such that the array of openings are aligned with the plurality...

20060046284 - Bgl4 beta-glucosidase and nucleic acids encoding the same: The present invention provides a novel β-glucosidase nucleic acid sequence, designated bgl4, and the corresponding BGL4 amino acid sequence. The invention also provides expression vectors and host cells comprising a nucleic acid sequence encoding BGL4, recombinant BGL4 proteins and methods for producing the same....

20060046283 - Dna encoding anti-apoptotic protein and recombinant 30k protein: The present invention relates to DNAs encoding anti-apoptotic 30K proteins. More particularly, the present invention is directed to 30K protein genes and a recombinant proteins prepared by using novel anti-apoptotic gene obtained from silkworm. The present invention also provides anti-apoptotic health care food, pharmaceutical preparation, additive for cell culture medium,...

20060046285 - Enhanced expression of fusion polypeptides with a biotinylation tag: The invention provides the means to enhance in E. coli-based expression systems the formation of fusion polypeptides containing as an N-terminal tag a biotinylation polypeptide. By way of specifically exchanging in the nucleic acid sequence encoding the biotinylation polypeptide nucleotides at 11 discrete positions enhances the formation of the total...

20060046286 - Method for preparing optically active beta-butyrolactones: The present invention is to provide a method for preparing easily and efficiently β-butyrolactones and/or optically active 3-hydroxycarboxylic acid derivatives in high optical purity by use of an easily available hydrolase, which are useful as intermediates for pharmaceuticals, agrochemicals and the like. More particularly, the present invention relates to a...

20060046287 - Enantioselective biotransformation for preparation of protein tyrosine kinase inhibitor intermediates: The invention relates to biocatalytic methods for preparing enantiomerically pure stereoisomers of 1-(2,6-dichloro-3-fluorophenyl)ethanol. Disclosed are methods of preparation of the desired (S)-enantiomer, which methods are based on a combination of enzymatic resolution, chemical esterification and chemical hydrolysis with inversion of 1-(2,6-dichloro-3-fluorophenyl)ethyl esters or stereoselective bio-reduction of 2,6-dichloro-3-fluoro-acetophenone with a biocatalyst...

20060046288 - Mutant e. coli strain with increased succinic acid production: The invention relates to a mutant strain of bacteria, which either lacks or contains mutant genes for several key metabolic enzymes, and which produces high amounts of succinic acid under anaerobic conditions....

20060046289 - Novel carbonyl reductase, gene thereof and use of the same: The present invention provides a novel polypeptide forming (R)-2-chloro-1-(3′-chlorophenyl)ethanol, a polynucleotide coding for said polypeptide, and use of the same. The present invention relates to a polypeptide having the following physical and chemical properties (1) to (4): (1) activity: acting on 2-chloro-1-(3′-chlorophenyl)ethanone with NADPH or NADH as a coenzyme, to...

20060046290 - Deactivation of linking moieties in antibody-enzyme conjugates: In some embodiments, the present invention pertains to a method for conjugating a first compound to a second compound wherein the conjugation involves an electrophilic moiety. The method comprises reacting the first compound with the second compound to form a conjugate. The improvement in embodiments of the present invention comprises...

20060046291 - Virus like particles, their preparation and their use preferably in pharmaceutical screening and functional genomics: The invention relates to virus like particles, their preparation and their use in pharmaceutical screening and functional genomics. The invention further provides a variety of assay formats to be used with said virus like particles....

20060046292 - Ferulate esterase producing strains and methods of using same: Ferulate esterase producing bacterial strains or functional mutants thereof and methods of using ferulate esterase producing bacterial strains as forage additives are disclosed....

20060046293 - Dopaminergic nurr1-positive neuron stem cells, pharmaceutical composition therefor, and methods for isolation, culture and preservation thereof: The present invention relates to a population of Nurr1-positive neuron stem cells, a pharmaceutical composition thereof, and methods for isolation, culture and preservation thereof. More specifically, the present invention provides an isolation method of Nurr1-positive neuron stem cells from human teeth. The said Nurr1-positive neuron stem cells can be applied...

20060046294 - Site-specific recombination systems for use in eukaryotic cells: Prokaryotic recombination systems have been adapted to function in eukaryotes in order to achieve one or more of the following: DNA site specific excision, translocation, integration and inversion. These recombination systems are identified as seven members of the small serine resolvase subfamily: CinH, ParA, Tn1721, Tn5053, Tn21, Tn402, and Tn501...

20060046295 - Helper virus-free aav production: A method for the production of adeno-associated virus stocks and recombinant adeno-associated virus stocks that are substantially free of contaminating helper virus is described. The method utilizes transfection with helper virus vectors to replace the infection with helper virus used in the conventional method....