Chemistry: molecular biology and microbiology inventions 03/06

20060068369 - Blood component separation method and apparatus: An apparatus and method for collecting whole blood and then separating it into components for subsequent use or storage. A self-contained bag set is used to collect the sample, which may then be placed into a device adapted to fit into a centrifuge for separation of components. Each component is...

20060068374 - Cell motility assay: An electrooptical method is disclosed for quantifying the motility response of cells to external stimuli, wherein a target cell population (formed as a function of said response) is subsequently optically differentiated by the addition of an optical differentiation solution, and whereby the targeted cell population becomes more or less detectable...

20060068370 - Liver specific chimeric regulatory sequence and use thereof: The present invention relates to a chimeric regulatory sequence with liver cell specificity. The chimeric regulatory sequence includes a proximal regulatory sequence and a distal enhancer of 5′ flanking region of human α-fetoprotein (AFP) gene. The chimeric regulatory sequence is useful in purified specific cell lineages, such as liver cells,...

20060068375 - Method and apparatus for computer controlled rare cell, including fetal cell, based diagnosis: A computer controlled method for detecting and diagnosing a rare cell type in a tissue sample is provided, said method comprising treating the tissue sample such that it generates a first signal indicative of the presence at a location of a rare cell, detecting the first signal, treating the location...

20060068376 - Method and apparatus for computer controlled rare cell, including fetal cell, based diagnosis: A computer controlled method for detecting and diagnosing a rare cell type in a tissue sample is provided, said method comprising treating the tissue sample such that it generates a first signal indicative of the presence at a location of a rare cell, detecting the first signal, treating the location...

20060068377 - Method and apparatus for computer controlled rare cell, including fetal cell, based diagnosis: A computer controlled method for detecting and diagnosing a rare cell type in a tissue sample is provided, said method comprising treating the tissue sample such that it generates a first signal indicative of the presence at a location of a rare cell, detecting the first signal, treating the location...

20060068372 - Method of classifying plant embryos using penalized logistic regression: A method is disclosed for classifying plant embryos according to their quality using a penalized logistic regression (PLR) model. First, sets of image or spectral data are acquired from plant embryos of known quality, respectively. Second, each of the acquired sets of image or spectral data is associated with one...

20060068371 - Methods for analyzing inter-cellular phenomena: Aspects of the present invention encompass the collection of multispectral images from a population of objects, and the analysis of the collected images to measure at least characteristic of the population, using photometric and/or morphometric features identifiable in the collection of images. In an exemplary application, the objects are biological...

20060068373 - Systems and methods of identifying biomarkers for subsequent screening and monitoring of diseases: A system for generating an image of ultrastructural biomarkers from a biological sample is provided. The system includes a grid onto which a sample to be imaged may be placed and a cryogenic reservoir into which the grid and sample may be immersed for vitrification of the sample. The system...

20060068380 - Assay for detecting and quantifying hiv-1: Compositions, methods and kits for detecting HIV-1 nucleic acids using nucleic acid amplification. Particularly described are oligonucleotides that are useful as hybridization probes and amplification primers for detecting very low levels of HIV-1 nucleic acids by real-time monitoring of amplicon production. The invented assays are characterized by high levels of...

20060068381 - Methods for identifying a peptide that binds a geometrical shape: Provided herein are methods, such as phage display assays, for bioengineering peptides that bind to geometrically and/or atomically structured molecular surfaces such as, for example, flat surfaces, smooth curved surfaces, as well as surfaces with periodic, random, or fractal atomic configurations. Surface-binding peptides are provided that are identified using the...

20060068378 - Nanoparticles having oligonucleotides attached thereto and uses therefor: The invention provides methods of detecting a nucleic acid. The methods comprise contacting the nucleic acid with one or more types of particles having oligonucleotides attached thereto. In one embodiment of the method, the oligonucleotides are attached to nanoparticles and have sequences complementary to portions of the sequence of the...

20060068379 - Phage display of intact domains at high copy number: Disclosed is a phage display system in which the molecules to be displayed (i.e., molecules of interest) are bound to dispensable capsid polypeptides such as SOC (small outer capsid) and HOC (highly antigenic outer capsid) polypeptides that are, in turn, bound to a surface lattice protein, such as those on...

20060068441 - Alternative pol kappa nucleotide and amino acid sequence and methods for using: The present invention provides isolated nucleic acid sequences and expression vectors encoding the pol κC76, substantially purified pol κ76, and methods for detecting pol κ76....

20060068432 - Biological markers for longevity and diseases and uses thereof: This invention provides methods of using of the sizes and levels of high-density lipoprotein (HDL) and low-density lipoprotein (LDL) particles, the −641 allele of the promoter of the gene encoding apolipoprotein C-3 (APOC-3), the 405 allele of the gene encoding cholesteryl ester transfer protein (CETP), and plasma levels of insulin-like...

20060068401 - Biopolymer resonant tunneling with a gate voltage source: The invention provides an apparatus and method for sequencing and identifying a biopolymer. The invention provides a first electrode, a second electrode, a first gate electrode, a second gate electrode, a gate voltage source and a potential means. The gate electrodes may be ramped by a voltage source to search...

20060068424 - Biosensor: An object of the present invention is to provide a biosensor wherein at least two types of surfaces are patterned without impairing the sensor surface and non-specific adsorption is thereby suppressed. The present invention provides a biosensor comprising a substrate coated with a hydrophobic compound having a photoactive group, or...

20060068411 - Cancer specific gene mh15: MH15 (Hn1L) is identified as an oncogene. Methods and compositions for detecting and diagnosing cancer in patients are provided, by determining the level of MH15 expression in biological samples. Also provided are methods for screening for inhibitors and moderators of MH15 expression and activity, as well as compositions comprising compounds...

20060068386 - Complete genome and protein sequence of the hyperthermophile methanopyrus kandleri av19 and monophyly of archael methanogens and methods of use thereof: We have determined the complete 1,694,969 nucleotide sequence of the GC-rich genome of Methanopyrus kandleri using a novel approach. It is based on unlinking genomic DNA with the ThermoFidelase version of M. kandleri topoisomerase V and cycle sequencing directed by 2′-modified oligonucleotides (Fimers). 3.3× sequencing redundancy was sufficient to assemble...

20060068415 - Complexity management of genomic dna by locus specific amplification: The present invention provides for novel methods and kits for reducing the complexity of a nucleic acid sample to interrogate a collection of target sequences. In one embodiment complexity reduction can be accomplished by extension of a locus specific capture probe followed by amplification of the extended capture probe using...

20060068436 - Compositions and methods for cdna synthesis: Methods for making cDNA molecules, for amplification of RNA by PCR and for preparation of cDNA libraries are provided. Kits for making cDNA molecules also are provided. Compositions are also provided comprising mixtures of reagents, including reverse transcriptases, buffers, cofactors and other components, suitable for immediate use in conversion of...

20060068421 - Compositions and methods for phage display of polypeptides: The present invention relates in part to novel polypeptide display methods that can permit the efficient display and selection of polypeptides, and to display vectors and other compositions configured for efficient use in such methods....

20060068426 - Cyclic peptides and antibodies thereof: The present invention provides methods of preparing and identifying antibodies against a loop domain of a protein, such as an extracellular loop (ECL) domain of a transmembrane protein. Cyclic and end-to-end cyclized peptides corresponding to loop domains are employed in the present invention. Transmembrane proteins contemplated by the invention include...

20060068419 - Detection of nucleic acids to assess risk for bovine spongiform encephalopathy: The present invention provides a method of detecting abnormal serum nucleic acid profiles to assess the risk of a transmissible spongiform encephalopathy, e.g., BSE....

20060068400 - Device and method for fecal testing: A specimen testing device having a first panel with at least two apertures, a second panel with at least two apertures opposite the apertures in said first panel, a sheet disposed between the first and second panels for receiving a specimen through the apertures, the sheet in the apertures in...

20060068416 - Devices coated with substances which mediate the adhesion of biological material: Devices having at least one surface which comes into contact with tissues and/or fluids of the human or animal body. These surfaces are at least partially coated with aptamers, which mediate the adhesion of biological material....

20060068390 - Dna amplification and sequencing in collapsible emulsions: The present invention relates to a method of performing a chemical reaction, in particular a small-scale chemical reaction. The method involves the use of two (or more) phases which, when formed into an emulsion, have the characteristic of being subject to “collapse” under certain physical or chemical conditions such that...

20060068422 - Gene chip technology for determining memory genes: The present invention relates to methods of identifying genes involved in memory formation. This is accomplished by performing a gene chip identification of those genes expressed during transcription-dependent memory formation but not during transcription-independent memory formation. A statistical analysis of the gene chip identification output yields a set of genes...

20060068425 - Genes, compositions, kits, and methods for identification, assessment, prevention, and therapy of prostate cancer: The invention relates to newly discovered nucleic acid molecules and proteins associated with prostate cancer including pre-malignant conditions. Compositions, kits, and methods for detecting, characterizing, preventing, and treating human prostate cancers are provided....

20060068387 - Genetic marker for endocrine disorders: A genetic marker associated with polycystic ovary syndrome is disclosed. The presence or absence of the allele is highly predictive of whether an individual is at risk from polycystic ovary syndrome. Methods of diagnosis, markers, and primers are disclosed and claimed in accordance with the present invention....

20060068397 - High throughput screening method for identifying molecules having biocidal function: Novel methods for screening and cloning DNA sequences coding for biocidal molecules, and for identifying biocidal molecules involving use of viability staining assay are provided. The methods consist of construction of libraries of DNA from natural and synthetic sources, and expression of cloned DNA in surrogate hosts, identification of DNA...

20060068414 - Identification of aging genes through large-scale analysis: High throughput methods for screening genetic variants that are phenotypically distinguishable are provided. Methods for identifying “long-lived” genetic variants among a set of variants are also provided. Methods for identifying pharmaceutical compounds that can promote longevity in various subjects, including mammals, and that can delay the onset of various diseases...

20060068438 - Identification of gene expression alterations underlying the aging process in mammals: A method of measuring the biological age of a multicellular organism is disclosed. In one embodiment, the method comprises the steps of: (a) obtaining a sample of nucleic acid isolated from the organism's organ, tissue or cell, wherein the nucleic acid is RNA or a cDNA copy of RNA and...

20060068428 - Identification of genetic markers associated with parkinson disease: The present invention provides methods and compositions for screening a subject for Parkinson disease, for increased risk of developing Parkinson disease and/or for an earlier or later age of developing Parkinson disease, comprising detecting the presence of a genetic marker associated with Parkinson disease....

20060068418 - Identification of markers in lung and breast cancer: Methods for identifying expression of markers indicative of the presence of breast cancer and lung cancer are provided. Also provided are articles of manufacture useful in such methods and compositions containing primers and probes useful in such methods....

20060068384 - Immuno polymerase chain reaction assay: A method to detect biological molecules in a sample which is an immuno polymerase chain reaction assay which comprises the detection of a complex which includes a ligand: nucleic acid conjugate bound to at least one biological molecule. The complex is detected by the addition of a second nucleic acid...

20060068412 - Integrated multistep bioprocessor and sensor: The invention provides an integrated biosensor. The integrated bioprocessor consists of an integrated capture chamber having an analyte recognition coating and a structure supporting analyte detection, analyte growth and target nucleic acid detection. The integrated capture chamber can consist of a waveguide, a capillary tube, a mixing flow chamber or...

20060068382 - Isolated human transporter proteins, nucleic acid molecules encoding human transporter proteins, and uses thereof: The present invention provides amino acid sequences of peptides that are encoded by genes within the human genome, the transporter peptides of the present invention. The present invention specifically provides isolated peptide and nucleic acid molecules, methods of identifying orthologs and paralogs of the transporter peptides, and methods of identifying...

20060068383 - Kits for the detection of target sequences: The present invention relates to means for cleaving a nucleic acid cleavage structure in a site-specific manner. Enzymes, including 5′ nucleases and 3′ exonucleases, are used to detect and identify nucleic acids derived from microorganisms. Methods are provided which allow for the detection and identification of bacterial and viral pathogens...

20060068429 - Leprotl1 and ob-rgrp genes for screening compounds that act on the gain or loss of weight or on diabetes in a human or animal subject: A method for identifying compounds acting on weight gain or weight loss or on diabetes in a human or animal subject including measuring effect of the compounds on (a) expression of at least one of genes LEPROTL1 and OB-RGRP or a part of them and/or (b) intracellular transport up to...

20060068403 - Long oligonucleotide arrays: Long oligonucleotide arrays, as well as methods for their preparation and use in hybridization assays, are provided. The subject arrays are characterized in that at least a portion of the probes of the array, and usually all of the probes of the array, are long oligonucleotides, e.g. oligonucleotides having a...

20060068437 - Meg-3 protein: The present invention provides a DNA expressed at high frequency in mesangial cells and a protein (Meg-3) encoded by this DNA. These substances are useful in, for example, identifying mesangial cells and detecting abnormalities in mesangial cells. Moreover, the above protein would be helpful for clarification of the functions of...

20060068396 - Method for biochip detecting limited cells: The present invention is a method for a biochip detecting limited cells. The present invention comprises steps of: obtaining a nylon membrane chip having required nucleotide fragments arranged in a dot matrix way by using a manual spotter; naturally drying by heat and fixing the nucleotide fragments on the nylon...

20060068408 - Method for determining dna damage and multi-chamber plate: In a method for determining DNA damage, wherein cells are treated with genotoxic agents and lysed and DNA fragments of the cells are separated according to their length by a physical separating method, cells are put into various chambers of a multi-chamber plate and a genotoxic chemicals are put into...

20060068435 - Method for screening cells and method for detecting oral carcinoma cells: The present invention provides a method of detecting oral carcinoma cells with high accuracy, and a method of making possible early detection of oral carcinoma or discrimination between a precancerous lesion and an early cancer in diagnosis of oral carcinoma. The methods are achieved by screening cells for oral carcinoma...

20060068442 - Method for the identification of extracellular domains of epstein barr virus (ebv) tumor-associated latent membrane proteins and for the selection of antibody reagents reactive therewith: Described are a method for the identification of extracellular epitopes of Epstein Barr virus (EBV) encoded membrane proteins, expressed on the outer cell surface of EBV-transformed mammalian cells, methods fo rthe selection and preparation of antibody reagents specific for the said epitopes, as well as peptides, including extracellular domains of...

20060068388 - Method for the identification of modulators of a secretase activity: The present invention relates to a cellular screening method for the identification of modulators of secretases. Said method comprises contacting suitable host cells with a test substance wherein said suitable host cells comprise: a) a fusion protein secretory protein, a membrane anchor domain and a secrease cleavage sequence, b) a...

20060068392 - Method of fixing biomolecule on metal support: A solution of a nucleic acid is spotted on a metal carrier consisting of a metal selected from Groups I, II, III, IV, V, VI, and VII of second to seventh periods and transition elements in a periodic table, or of an alloy containing the metal, and the solution is...

20060068407 - Method of immobilizing nucleic acid aptamers: The present invention relates to methods of immobilizing nucleic acid aptamers within a sol-gel matrix. The aptamer system remains functionally intact when it is immobilized within a protein and membrane-compatible sol-gel derived from polyol silane precursors or sodium silicate....

20060068394 - Method of producing a dna library using positional amplification: The disclosed invention relates to general and specific methods to use the Primer Extension/Nick Translation (PENT) reaction to create an amplifiable DNA strand, called a PENTAmer. A PENTAmers can be made for the purpose of amplifying a controlled length of DNA located at a controlled position within a DNA molecule,...

20060068434 - Methods and compositions for detecting cancer using components of the u2 spliceosomal particle: The present invention relates to cancer-associated proteins and nucleic acids that encode or bind specifically to cancer-associated proteins, which represent markers for cancer detection. Specifically, the invention provides a family of methods and compositions for detecting cancer, for example, breast cancer, in an individual using components of the U2 spliceosomal...

20060068389 - Methods and compositions for inhibiting hiv replication: Pharmaceutial composition comprising compounds and/or compositions useful to inhibit HIV replication are disclosed. Methods of treating individuals infected with HIV are disclosed. Methods of preventing HIV infection in high-risk individuals are disclosed....

20060068417 - Methods and compositions to detect nucleic acids in a biological sample: Methods of the invention separate a target nucleic acid from a sample by using at least one capture probe oligonucleotide that contains a target-complementary region and a member of a specific binding pair that attaches the target nucleic acid to an immobilized probe on a capture support, thus forming a...

20060068440 - Methods and device for dna sequencing using surface enhanced raman scattering (sers): The methods and apparatus disclosed herein concern nucleic acid characterization by enhanced Raman spectroscopy. In certain embodiments of the invention, exonuclease treatment of the nucleic acids results in the release of nucleotides. The nucleotides may pass from a reaction chamber through a microfluidic channel and enter a nanochannel or microchannel....

20060068405 - Methods and systems for annotating biomolecular sequences: Polypeptide sequences and polynucleotide sequences are provided. Also provided are annotative information concerning such sequences and uses for these sequences....

20060068410 - Methods and systems for monitoring intracellular binding reactions: Intracellular binding reactions, and particularly DNA/DNA binding protein reactions are detected in situ, using intracellular fluorescence polarization detection. The methods comprise providing a biological cell having at least a first component of a binding reaction disposed therein. The cell is contacted with a second component of the binding reaction whereby...

20060068402 - Methylated promoters of colon cancer-specific expression-decreased genes and use thereof: Methylated promoters of colon cancer-specific genes and use thereof. Various disclosed aspects of the invention include methylated promoters of the colon cancer specific expression-decreased genes, microarrays for cancer diagnosis on which the methylated promoters are immobilized, and cancer diagnosis kits containing the methylated promoters. The methylated promoters of colon cancer-specific...

20060068393 - Mevalonate kinase as a target for fungicides: The present invention relates to the provision of mevalonate kinase as target for fungicides, to the provision of novel nucleic acid sequences, of functional equivalents of the abovementioned nucleic acid sequences and to the use of the gene products of the abovementioned nucleic acid sequences as novel targets for fungicides....

20060068431 - Micro rt-pcr apparatus and method using the same: A micro RT-PCR apparatus includes a chip module and control module. The chip module includes a reaction chamber unit, and a heating unit for heating the reaction chamber unit. The control module controls the heating unit to perform a first heating operation and a second heating operation. The first heating...

20060068385 - Mononucleotide repeats microsatellite markers for detecting microsatellite instability: A method for evaluating microsatellite instability associated with a tumor, which entails the steps of amplifying microsatellite loci in a biological sample containing genomic DNA from the tumor and determining sizes of DNA amplification products, wherein at least one microsatellite locus selected from the group consisting of NR 21, NR...

20060068399 - Multiple bead reagent system for protein based assays with optimized matrices: The invention provides a multi-bead assay system for a protein based assay comprising at least two different beads. The first bead comprises protein and a protein stabilization matrix. The first bead forms a first solution when dissolved in liquid, and the first solution permits a first activity level for the...

20060068433 - Multiple mode multiplex reaction quenching method: Methods for balancing multiplexed PCR reactions are provided which exploit differences in primer and amplicon Tms. The methods may be controlled by a computer process. Also provided are articles of manufacture useful in such methods and compositions containing primers and probes useful in such methods....

20060068430 - Purification of biomolecules from contaminating intact nucleic acids: The present invention provides methods for the removal, destruction or inactivation of intact nucleic acids contaminating desired biomolecules by the use of small molecule nucleic acid cleavage agents....

20060068427 - Qualitative analysis of a sample using an algorithm: To determine the presence of a specific nucleic acid sequence within a sample, a labelled substance, capable of binding the nucleic acid to be determined, or a labelling substance is added, the substance having the ability to label the nucleic acid or being representative for the nucleic acid. The nucleic...

20060068420 - Regulation of th2 cell activity by modulation of nfatp and nfat4 activity: The invention demonstrates that NFATp and NFAT4 are required for the control of lymphocyte homeostasis and act as selective repressors of Th2 cells. The invention provides mice deficient in both NFATp and NFAT4 that exhibit a phenotype characteristic of increased Th2 cell activity. Methods for identifying modulators of Th2 cell...

20060068404 - Secreted and transmembrane polypeptides and nucleic acids encoding the same: The present invention is directed to novel polypeptides and to nucleic acid molecules encoding those polypeptides. Also provided herein are vectors and host cells comprising those nucleic acid sequences, chimeric polypeptide molecules comprising the polypeptides of the present invention fused to heterologous polypeptide sequences, antibodies which bind to the polypeptides...

20060068413 - Secreted and transmembrane polypeptides and nucleic acids encoding the same: The present invention is directed to novel polypeptides and to nucleic acid molecules encoding those polypeptides. Also provided herein are vectors and host cells comprising those nucleic acid sequences, chimeric polypeptide molecules comprising the polypeptides of the present invention fused to heterologous polypeptide sequences, antibodies which bind to the polypeptides...

20060068423 - Secreted and transmembrane polypeptides and nucleic acids encoding the same: The present invention is directed to novel polypeptides and to nucleic acid molecules encoding those polypeptides. Also provided herein are vectors and host cells comprising those nucleic acid sequences, chimeric polypeptide molecules comprising the polypeptides of the present invention fused to heterologous polypeptide sequences, antibodies which bind to the polypeptides...

20060068406 - Single-stranded nucleic acid template-mediated recombination and nucleic acid fragment isolation: Methods mediated by single-stranded nucleic acid templates, including utilizing single-stranded nucleic acid templates to isolate nucleic acid fragments and to recombine nucleic acid fragments. Methods include polymerase and polymerase-free recombination of nucleic acid fragments to generate chimeric nucleic acid sequences. Integrated systems and kits are also provided....

20060068395 - Synthetic nucleic acid molecule compositions and methods of preparation: A method to prepare synthetic nucleic acid molecules having reduced inappropriate or unintended transcriptional characteristics when expressed in a particular host cell....

20060068391 - Targeting of herpes simplex virus to specific receptors: The invention relates to engineered Herpes simplex virus (HSV) particles that are targeted to one or more specific receptors. Also, recombinant vectors for producing such HSV particles. By reducing the affinity of HSV for its natural receptor(s) and increasing the affinity for a selected receptor, the HSV particles of the...

20060068439 - Ucp4: The present invention is directed to novel polypeptides having homology to certain human uncoupling proteins (“UCPs”) and to nucleic acid molecules encoding those polypeptides. Also provided herein are vectors and host cells comprising those nucleic acid sequences, chimeric polypeptide molecules comprising the polypeptides of the present invention fused to heterologous...

20060068398 - Universal and target specific reagent beads for nucleic acid amplification: The present invention relates to methods and compositions providing optimized reaction conditions for nucleic acid amplification reactions and for methods and compositions that reduce contamination of amplification reactions during set up....

20060068409 - Use of restriction enzymes and other chemical methods to decrease non-specific binding in dual bead assays and related bio-discs, methods, and system apparatus for detecting medical targets: Methods for decreasing non-specific bindings of beads in dual bead assays and related optical bio-discs and disc drive systems. Methods include identifying whether a target agent is present in a biological sample and mixing capture beads each having at least one transport probe affixed thereto, reporter beads each having at...

20060068444 - Compositions and methods for detecting cyclic analogs of hpth: The present invention is directed to novel antigens, antibodies and antigen binding fragments thereof, and to immunoassays and kits using these antigens and antibodies. The compositions and methods are useful in the determination of levels of cyclic analogs of hPTH in sample fluids, such as serum or plasma. The antibodies...

20060068445 - Novel soluble cd14 antigen: There are provided a soluble CD14 antigen which is a novel in vivo protein useful as a marker for diagnosing sepsis and has the following characteristic features 1) to 3): 1) a molecular weight of 13±2 kDa when measured by SDS-PAGE under non-reducing conditions; 2) an amino acid sequence in...

20060068443 - Universal binding matrix for creating pairing ligands for identifying proteomic interaction: A Universal Binding Matrix is described which binds various classes of combinatorial libraries. This bound library can then be probed with test species of a simple or complex nature to identify complementary test species as well as test species surface mimics. These complementary test species have the characteristic of binding...

20060068447 - Directed assembly of functional heterostructures: The present invention relates to a systematic process for the creation of functionally organized, spatially patterned assemblies polymer-microparticle composites including the AC electric field-mediated assembly of patterned, self supporting organic (polymeric) films and organic (polymeric)-microparticle composite films of tailored composition and morphology; the present invention further relates to the incorporation...

20060068446 - Methods: A method for identifying a compound for modulating the immune response in a first type of mammal to a super-antigen, comprising the steps of 5 (7) providing a cell expressing a MHC Class II molecule derivable at least in part from the first type, or cells comprising such a cell...

20060068451 - Dimeric fluorescent polypeptides: The invention relates to proteins or polypeptides that comprise intramolecular dimers of fluorescent protein monomers. More specifically, the invention relates to recombinant polypeptides comprising a monomer of a fluorescent polypeptide, a linker peptide, and a second monomer of that fluorescent polypeptide, where the monomers form an intramolecular dimer. The invention...

20060068452 - Differential protein expression patterns related to disease states: The present invention is a method for determining protein expression profiles in disease or an altered biological state. The method is based on the use of two-dimensional (2D) gel electrophoresis where the gel images are assigned two different colors. The gels are then compared by an overlay procedure that allows...

20060068453 - Survivin, a protein that inhibits cellular apoptosis, and its modulation: The present invention provides the amino acid of a protein that inhibits cellular apoptosis, herein termed the Survivin protein and nucleic acid molecules that encode Survivin. Based on this disclosure, the present invention provides isolated Survivin protein, isolated Survivin encoding nucleic acid molecules, methods of isolating other members of the...

20060068448 - Fungal antigens and process for producing the same: There can be provided a fungal antigen which is an insoluble fraction obtainable from fungal cells of which cell wall has been substantially removed or at least partially removed; a process for producing the same; a nucleic acid encoding the fungal antigen; a biologic product containing the fungal antigen; a...

20060068449 - N-terminal and c-terminal markers in nascent proteins: This invention relates to non-radioactive markers that facilitate the detection and analysis of nascent proteins translated within cellular or cell-free translation systems. Nascent proteins containing these markers can be rapidly and efficiently detected, isolated and analyzed without the handling and disposal problems associated with radioactive reagents. Methods are described for...

20060068450 - Microbead-filled microsystem and production method thereof: The invention relates to a micro-system intended to receive beads and to obtain a precise positioning of the beads at preset locations in the micro-system. This micro-system comprises a tank that has a cavity, the cavity being fitted with blocking elements allowing the beads to be ordered and stacked in...

20060068454 - Method of inactivating blood coagulation factor and blood coagulation factor-inactivated sample: A method of inactivating a blood coagulation factor is described. The method comprises a step of contacting a sample containing at least one of factors V and VIII with a compound having an iminodiacetate group, whereby at least one of factors V and VIII in the sample is changed into...

20060068455 - Gaba detection methods and applications: Disclosed herein are silica glass sensors comprising more than one active biological material in a single sol-gel layer. The silica glass sensors may be used to assay complex biological mechanisms in cells and tissues cultivated thereon. Also disclosed are silica glass sensors having GABA-T, SSADH, or both, for detecting GAD,...

20060068456 - Methods for screening and identifying compounds: The present invention relates to methods for assessing enzyme activity utilizing a phase partition system. In addition, the present invention also relates to methods for screening and identifying compounds that may be used, for example, for the treatment of diabetes, diabetes-related disorders, obesity, cardiovascular disease, cancer, and other diseases or...

20060068457 - Substrates for tafi (a): The invention relates to compounds of general formula (I) and acid addition salts thereof, where the various symbols have the meanings given in the description and claims, the production and use thereof as substrate for the detection of TAFIa, a fibrinolysis inhibiting enzyme. The detection occurs by using the absorption...

20060068458 - Coupled enzymatic reaction system using a formate dehydrogenase derived from candida boidinii: The present process relates to a coupled enzymatic reaction system which is implemented in a two-phase system consisting of organic phase and aqueous phase. The system operates with cofactor-dependent enzymes, the cofactor being continually regenerated enzymatically by an FDH derived from C. boidinii....

20060068459 - Methods for detecting xanthrochromia: The invention provides methods and compositions for making a diagnosis of Xanthrochromia in patient-derived samples of cerebrospinal fluid....

20060068460 - Biochemical synthesis apparatus: A biochemical synthesis apparatus comprises a receptacle for containing a medium, and a support on which a microorganism can be placed. The support can be placed in contact with the medium, so as to allow access of the microorganism to the medium. The support with the microorganism can be removed...

20060068462 - Method: A method of producing one or more of a carbohydrate ester, a protein ester, a protein subunit ester or a hydroxyl acid ester, which method comprises admixing an acyl donor, an acyl acceptor and water to produce a high water environment comprising 5-98% water, wherein said acyl donor is a...

20060068461 - Transglycosylation method and protein having transglycosylation activity: The present invention provides a transglycosylation method and a protein catalyzing a transglycosylation reaction. A reaction of transferring a galactosylβ1→3N-acetylgalactosaminyl group from a sugar donor containing a sugar chain having a galactosylβ1→3N-acetylgalactosaminyl group bound thereto at the α-position, to a sugar chain acceptor, is carried out in the presence of...

20060068468 - Actrii receptor polypeptides, methods and compositions: In certain aspects, the present invention provides compositions and methods for modulating (promoting or inhibiting) growth of a tissue, such as bone, cartilage, muscle, fat, and/or neuron. The present invention also provides methods of screening compounds that modulate activity of an ActRII protein and/or an ActRII ligand. The compositions and...

20060068470 - Antifreeze proteins from basidiomy cetes: The present invention provides antifreeze proteins produced by a basidiomycete. The antifreeze protein has a high antifreeze activity such as a thermal hysteresis activity or an ice-recrystallization inhibition activity....

20060068469 - Bacterial vector systems: The present invention provides bacterial vectors and fusion proteins containing a TTSS polypeptide, compositions of such fusion proteins including polynucleotides, and methods of delivering one or more genes into a target cell that involve contacting the cell with a composition that includes such a fusion protein. Compositions and methods of...

20060068472 - Compositions to ameliorate protein misfolding and aggregation: The invention relates to polynucleotides comprising polynucleotide sequences corresponding to the tor-1, tor-2, ooc-5, DYT1, and DYT2 genes and parts thereof that encode polypeptide sequences and parts thereof possessing varying degrees of torsin activity, and methods of screening and amplifying polynucleotides encoding polypeptide sequences which encode polypeptides having varying degrees...

20060068463 - Natural killer cell activating factor ii: The invention relates to NKAF II polypeptides, polynucleotides encoding the polypeptides, methods for producing the polypeptides, in particular by expressing the polynucleotides, and agonists and antagonists of the polypeptides. The invention further relates to methods for utilizing such polynucleotides, polypeptides, agonists and antagonists for applications, which relate, in part, to...

20060068466 - Novel g protein-coupled receptor: The present invention is directed to novel G protein-coupled receptors that are found predominantly in the dorsal root ganglia. The invention encompasses both the receptor proteins as well as nucleic acids encoding the proteins. In addition, the present invention is directed to methods and compositions which utilize the receptors....

20060068464 - Regulation of human g protein coupled receptor: Reagents which regulate human G protein-coupled receptor and reagents which bind to human GPCR gene products can play a role in preventing, ameliorating, or correcting dysfunctions or diseases including, but not limited to, infections such as bacterial, fungal, protozoan, and viral infections, particularly those caused by HIV viruses, pain, cancers,...

20060068467 - Rhodococcus nitrile hydratase: The invention relates to a Rhodococcus polynucleotide cluster which contains nucleotide sequences which encode polypeptides having the activity of a nitrile hydratase, of an auxiliary protein P15K which activates this enzyme and of a cobalt transporter, to transformed microorganisms in which the nucleotide sequences encoding these proteins are present in...

20060068471 - Soluble zcytor 11 cytokine receptors: Novel polypeptide combinations, polynucleotides encoding the polypeptides, and related compositions and methods are disclosed for soluble zcytor11 receptors that may be used as novel cytokine antagonists, and within methods for detecting ligands that stimulate the proliferation and/or development of hematopoietic, lymphoid and myeloid cells in vitro and in vivo. Ligand-binding...

20060068465 - Ubiquitin-specific protease occurring in the brain and dna encoding the same: A ubiquitin-specific protease occurring in the brain and a DNA encoding it, which are useful for research on the molecular mechanism of the neuroplasticity expression and so on....

20060068473 - Lactobacillus n-deoxyribosyl transferases, corresponding nucleotide sequences and their uses: The invention concerns novel polypeptides and their fragments, isolated from Lactobacillus, having at least a N-deoxyribosyl transferase activity, the polynucleotides encoding said polypeptides, cloning and/or expression vectors including said polynucleotides, cells transformed by said vectors and specific antibodies directed against said polypeptides. The invention also concerns a method for enzymatic...

20060068474 - Endothelial-monocyte activating polypeptide iii: A human EMAP III polypeptide and DNA (RNA) encoding such polypeptide and a procedure for producing such polypeptide by recombinant techniques is disclosed. Also disclosed are methods for utilizing such polypeptide for preventing and/or treating neoplasia. Diagnostic assays for identifying mutations in nucleic acid sequence encoding a polypeptide of the...

20060068475 - Pre-treatment of bales of feedstock: The present invention is directed to a method of pre-treating a lignocellulosic feedstock. The lignocellulosic feedstock comprises cereal straw, stover, or grass. One or more than one bale of lignocellulosic feedstock is conveyed into a pre-treatment reactor. Steam and acid are added to the bales and are maintained at a...

20060068476 - Method for the production by fermentation of sulphur-containing fine chemicals (metf): The invention relates to methods for the fermentative production of sulfur-containing fine chemicals, in particular L-methionine, by using bacteria which express a nucleotide sequence coding for a methionine synthase (metF) gene....

20060068477 - Methods for the production of products in host cells: The invention provides methods and host cells for the production of ascorbic acid intermediates. The invention also provides host cells having a modification in a polynucleotide that uncouples the catabolic pathway from the oxidative pathway by deleting the encoding for an endogenous enzymatic activity that phosphorylates D-glucose at its 6th...

20060068479 - Porcine forssman synthetase protein, cdna, genomic organization, and regulatory region: The present invention provides porcine Forssman synthetase (FSM synthase) (Globoside α-N-acetylgalactosaminyltransferase) protein, cDNA, and genomic DNA sequence. Furthermore, the present invention includes porcine animals, tissue and organs as well as cells and cell lines derived from such animals, tissue and organs, which lack expression of functional FSM synthetase. Such animals,...

20060068478 - Site specific incorporation of keto amino acids into proteins: Compositions and methods of producing components of protein biosynthetic machinery that include orthogonal tRNAs, orthogonal aminoacyl-tRNA synthetases, and orthogonal pairs of tRNAs/synthetases, which incorporate keto amino acids into proteins are provided. Methods for identifying these orthogonal pairs are also provided along with methods of producing proteins with keto amino acids...

20060068480 - Polypeptides and polypeptide analogues: m

20060068481 - Human kinases: The invention provides human kinases (PKIN) and polynucleotides which identify and encode PKIN. The invention also provides expression vectors, host cells, antibodies, agonists, and antagonists. The invention also provides methods for diagnosing, treating, or preventing disorders associated with aberrant expression of PKIN....

20060068483 - Extracellular serine protease: The present invention provides a DNA encoding a TADG-14 protein selected from the group consisting of: (a) isolated DNA which encodes a TADG-14 protein; (b) isolated DNA which hybridizes to isolated DNA of (a) above and which encodes a TADG-14 protein; and (c) isolated DNA differing from the isolated DNAs...

20060068482 - Taspase1 and methods of use: A novel protease, Taspase1, is described. Taspase1 is involved in the cleavage of the MLL protein, which is required for proper HOX gene regulation. Diagnostic methods utilizing Taspase1 are provided, as well as inhibitors of Taspase1. Methods of using the inhibitors of Taspase1 are also described. For example an inhibitor...

20060068484 - Enteropathogenic e. coli is a separate genus pathogenic bacteria: The invention is intended for treatment of diseases in accordance with immunity deficiency (HIV/AIDS diseases and other), oncological ailments, diseases connected with aggresive autoantibody (Diabetes and other autoimmune diseases), tuberculosis and etc. Purpose of the invention is the reinforcement of immunological reactivity organism. To this effect it is offered to...

20060068485 - Novel human synaptotagmin and polynucleotides encoding the same: Novel human polynucleotide and polypeptide sequences are disclosed that can be used in therapeutic, diagnostic, and pharmacogenomic applications....

20060068486 - Pet waste disposal assembly and method: An assembly adapted to facilitate the disposing and treating of a biodegradable material that produces an unpleasant odor when it is watered and stirred and fragmented. The assembly includes a container and a fitting for accepting and retaining the insertive coupler of a standard garden hose. In addition, a pressure...

20060068489 - Apparatus and method of assay in utilizing attenuated total reflection: An assay apparatus is loaded with a sensor unit as surface plasmon resonance (SPR) biosensor, which includes a transparent dielectric medium and thin film. A first surface of the thin film is a thin film/dielectric interface. Its sensing surface causes reaction of sample fluid. An optical assay unit assays reaction...

20060068487 - Nanostructured surface for microparticle analysis and manipulation: The present invention provides an apparatus, comprising a first mechanical structure having a first rigid surface, an area of the first rigid surface having a nanostructured surface. The apparatus also includes a second mechanical structure having a second rigid surface and opposing the first mechanical structure. The second rigid surface...

20060068488 - Screening apparatus: A screening apparatus detects images of a plurality of wells provided at a plate to perform screening in a new drug process. The screening apparatus has a plurality of detecting portions which are fixedly arranged and individually detect images of wells different from each other, a movable stage on which...

20060068490 - Flow-through chemical and biological sensor: The invention provides a mixing flow apparatus. The mixing flow apparatus consists of a waveguide and a mixing flow chamber; the waveguide having a higher index of refraction material than its surroundings for propagation of a signal, and the mixing flow chamber having a body forming a flow chamber with...

20060068491 - Microdevice for performing method of separating and purifying nucleic acid: A microdevice for performing a method for separating and purifying a nucleic acid, the microdevice comprising: at least one opening; and at least one channel for passing a sample solution, wherein the method comprises: (A) a step of bringing a nucleic acid-containing sample solution into contact with a nucleic acid-adsorbing...

20060068492 - Hybrid bioreactor for cell culture: A hybrid bioreactor for cell culture is disclosed. To simultaneously apply compressive strain for cell differentiation and shear strain for cell proliferation to cells, the hybrid bioreactor includes a plurality of reactor tube assemblies (100), a compressive strain motor (5), a shear strain motor (25), a lower anchor mount (20)...

20060068493 - Protein activity screening of clones having dna from uncultivated microorganisms: Disclosed is a process of screening clones having DNA from an uncultivated microorganism for a specified protein, e.g. enzyme, activity by screening for a specified protein, e.g. enzyme, activity in a library of clones prepared by (i) recovering DNA from a DNA population derived from at least one uncultivated microorganism;...

20060068494 - Wnt4 in supporting lymphopoiesis: The T lineage differentiative potential of lymphoid progenitor cells in the lymph nodes is thwarted by the absence of Wnt signaling, resulting in blockade of the DN1→DN2 transition with accumulation of pre-DN2 cells. Wnt4 transcripts are deficient in the lymph nodes relative to the thymus. When cultured with stromal cells...

20060068495 - Process of cell electrofusion: The invention relates to the use of an electrical field applied to a mixture containing a first type of cell (C1), a second type of cell (C2), a bispecific ligand able to bind to C1 and/or to C2 and non-covalent complexes formed between C1, C2 and the bispecific ligand, for...

20060068496 - Differentiation of stem cells: Disclosed are compositions and methods for identifying specific cell types....

20060068497 - Methods for isolating novel antimicrobial agents from hypermutable mammalian cells: Dominant-negative alleles of human mismatch repair genes can be used to generate hypermutable cells and organisms. By introducing these genes into mammalian cells new cell lines with novel and useful properties can be prepared more efficiently than by relying on the natural rate of mutation or introduction of mutations by...

20060057552 - Method of conserving biological prostheses, conserved biological prostheses and conserving solution: The invention concerns a method of conserving biological prostheses, wherein the method includes the following steps: (a) treating biological prostheses with a solution which contains a mixture of epoxide compounds which are at least in part of different lengths; (b) treating the biological prosthesis treated in accordance with step (a)...

20060057553 - Chimeric viral vectors for gene therapy: The invention relates to a single nucleic acid vector comprising both adenoviral and retroviral sequences for gene therapy. The vectors described herein (See FIG. 2) are capable of transducing all cis and trans components of a retroviral vector for the generation of high titer recombinant retroviral vectors. The chimeric vectors...

20060057556 - Contiguous capillary electrospray sources and analytical devices: Contiguous capillaries useful for separating and electrospraying a fluid comprising analyte and electrolyte are provided. The contiguous capillaries have spray tips at one end of the capillaries and electrically conductive portions in proximity to the spray tips. Methods for making the contiguous capillaries and their use as electrospray sources are...

20060057559 - High-throughput cell migration screening assay: The invention relates to methods for identifying and using agents, including small organic molecules, antibodies, peptides, cyclic peptides, nucleic acids, antisense nucleic acids, sphingolipid analogs, and ribozymes, that modulate cell activation or migration, e.g., lymphocyte migration, via modulation of the expression and/or activity of migration molecules such as, for example,...

20060057555 - Method and system for controlling the development of biological entities: A method and system are presented for use in controlling the processing of components, e.g. biological entities. Each component is assigned with a unique machine readable identification mark. Data records are provided representative of matching sets of the identification marks relating to at least two associated components. Each component-containing holder...

20060057557 - Method for identifying an image of a well in an image of a well-bearing component: A method for identifying images of wells in an image of a well-bearing object such as multiwell plates or picowell carriers is provided. An observation component, such as a camera, is used to approach focus of a focal point of a well-bottom. An image of the focal point is acquired....

20060057558 - Method to measure the effect of topically applied agents using skin maintained in organ culture: The method employs a piece of skin of area greater than 1 cm2 cultured under conditions that maintain its viability and substantially normal structure for sufficient time for topically applied test material to potentially exert an effect. The skin includes the majority of the epidermal layer plus an appropriate amount...

20060057554 - Sample collecting device and mass spectrometry of device: A sample collection device comprising a support bearing an inert absorbing matrix for a fluid sample is described. The device may or may not have a lancet. Also described for a sample device is a method of using a mass spectrometer in a laboratory where the sample in its matrix...

20060057560 - System and method for denaturing and fixing collagenous tissue: A method for modifying a geometry of a collagenous tissue mass includes heating the collagenous tissue mass to a temperature sufficient to cause denaturation, and introducing a biocompatible fixative, such as genepin, into the collagenous tissue mass....

20060057562 - Method, composition and kit for antigenic binding of norwalk-like viruses: A method for detecting a Norwalk-Like Virus (NLV) in a biological sample, comprising the steps of: obtaining a biological sample suspected of containing a NLV; contacting the biological sample with at least one human histo-blood group antigen to allow formation of a complex of the NLV with the antigen; and...

20060057563 - Peptides derived from capsid antigens of the epstein-barr virus and the use thereof: In the area of virus diagnosis, in particular Epstein-Barr virus (EBV) diagnosis, methods for the detection of EBV and agents suitable for this purpose are provided, including peptides which are derived from p18-VCA and permit discrimination between acute and past EBV infection....

20060057561 - Virus detection method, primers therefor and screening kit: Discloses a novel detection and typing method for viruses, such as human papillomaviruses, based on real-time PCR using self-probing amplicon fluorescent primers. The method comprises: (IA) contacting the sample with a self-probing amplicon (‘virus self-probing amplicon’) comprising (i) a virus primer capable of hybridising to at least one target viral...

20060057582 - 83 human secreted proteins: The present invention relates to novel human secreted proteins and isolated nucleic acids containing the coding regions of the genes encoding such proteins. Also provided are vectors, host cells, antibodies, and recombinant methods for producing human secreted proteins. The invention further relates to diagnostic and therapeutic methods useful for diagnosing...

20060057596 - Activity-based assay for ricin-like toxins: A method of detecting N-glycosylase activity in a sample involves incubating an oligodeoxyribonucleotide substrate containing a deoxyadenosine or deoxyuridine residue with the sample to be tested such that the N-glycosylase, if present, hydrolyzes the deoxyadenosine or deoxyuridine residue to result in an N-glycosylase product having an abasic site. A primer...

20060057624 - Alternatively spliced pre-mrna transcripts in neurodegenerative disease: The present invention provides a method of diagnosing a neurodegenerative disease in a mammalian subject, preferably a human subject. The method comprises obtaining RNA from the mammalian subject, and assaying the RNA for an increase in the amount of ΔFosB mRNA or in ratio of the amount of ΔFosB mRNA...

20060057617 - Amplification process: A method for conducting a nucleic acid amplification reaction, said method comprising forming an amplification reaction mixture in the presence of sufficient of a pyrophosphate salt to prevent primer extension taking place, digesting said pyrophosphate salt with a pyrophosphatase enzyme (PPase), and subjecting said reaction mixture to conditions such that...

20060057570 - Antisense modulation of hematopoietic cell protein tyrosine kinase expression: Antisense compounds compositions and methods are provided for modulating the expression of hematopoietic cell protein tyrosine kinase. The compositions comprise antisense compounds, particularly antisense oligonucleotides, targeted to nucleinc acids encoding hematopoietic cell protein tyrosine kinase. Methods of using these compounds for modulation of hematopoietic cell protein tyrosine kinase expression and...

20060057621 - Assay for detecting tuberculosis in nonhuman primates: Systems, methods, and compositions for a diagnostic method for detecting TB in non-human primates that is easy to use, sensitive, and specific. The method utilizes recombinant mycobacterial antigens, such as polyfusion proteins. The method utilizes an antigen-antibody-antigen arrangement to detect TB infection in nonhuman primates. The method can detect IgM...

20060057626 - Assessment of ctla-4 polymorphisms in ctla-4 blockade therapy: The invention provides methods for predicting responsiveness of a subject to therapy with a CTLA-4 blocking agent. The methods involve assaying for at least one CTLA-4 polymorphism in the subject and predicting responsiveness of the subject to therapy with a CTLA-4 blocking agent based on presence or absence of a...

20060057580 - Biochip with maximization of the reactor number: This invention concerns a biochip, consisting primarily of one or several dieplates and one or several substrates with or without probe immobilized, and comprising a maximized number of reactors, wherein: a. said maximization of said reactor number is performed by minimizing structure-covered area on the substrate and/or maximizing effective area...

20060057589 - Biomarkers for toxic algae: The present invention is directed toward biomarkers that identify characteristics of algae. The invention is further directed toward biomarkers that serve to identify algae species and strains of algae species as well as detect the presence of algal toxins. Additional embodiments feature methods utilizing algal biomarkers and polypeptides that can...

20060057565 - Combinatorial fluorescence energy transfer tags and uses thereof: This invention provides a combinatorial fluorescence energy transfer tag which comprises a plurality of fluorescent molecules, comprising one or more energy transfer donor and one or more energy transfer acceptor, linked through a molecular scaffold wherein the fluorescent molecules are separated along the scaffold to produce a unique fluorescene emission...

20060057605 - Compositions for use in identification of viral hemorrhagic fever viruses: The present invention provides oligonucleotide primers, compositions, and kits containing the same for rapid identification of viruses that cause viral hemorrhagic fevers by amplification of a segment of viral nucleic acid followed by molecular mass analysis....

20060057595 - Compositions, methods, and kits for identifying and quantitating small rna molecules: Compositions, methods, and kits for identifying and for quantitating polynucleotide targets are disclosed. These compositions, methods, and kits are particularly useful when the polynucleotide target is a small RNA molecule, including without limitation microRNA (miRNA), small interfering RNA (siRNA), and certain other classes of non-coding RNA molecules. The forward and...

20060057603 - Detecting bacillus anthracis: The present invention utilizes unique DNA signatures to identify Bacillus anthracis. The DNA signatures can also be used in other types of detection. These signatures have been found to be unique to Bacillus anthracis, and can be used to understand how these DNA regions are being used to make this...

20060057578 - Determination of an analyte in a liquid medium: The present invention concerns a magneto-controlled method and system for the determination of an analyte in a liquid medium. The method and system of the invention are based on the use of functionalized magnetic particles, e.g. magnetic particles that carry a recognition agent, such that in the presence of the...

20060057618 - Determining data quality and/or segmental aneusomy using a computer system: A method and/or system for making determinations regarding samples from biologic sources including statistical methods for making meaning grouping of observed data and/or for determining an overall quality measure of an assay....

20060057629 - Device for injecting pcr solution into pcr channels of pcr chip, and pcr chip unit including the device: Provided is a device for injecting a PCR solution into PCR channels of a PCR chip. The device includes: first through-holes corresponding to each of the inlets of the PCR chip in which PCR channels are formed; and second through-holes corresponding to each of the outlets of the PCR chip....

20060057577 - Diagnostics and therapeutics for diseases associated with homo sapiens formyl peptide receptor-like 2: The invention provides a human FPRL2 which is associated with the cardiovascular diseases, cns disorders, hematological diseases, genito-urinary diseases, cancer and respiratory diseases. The invention also provides assays for the identification of compounds useful in the treatment or prevention of cardiovascular diseases, cns disorders, hematological diseases, genito-urinary diseases, cancer and...

20060057584 - Endometriosis-related markers and uses thereof: The present invention relates to markers of endometriosis which are differentially expressed in the endometrial cells of females with endometriosis compared to endometriosis-free females. The invention also relates to methods for determining likelihood of endometriosis in female subjects, to methods for grading endometriosis in females suffering from endometriosis and to...

20060057601 - Genomic markers of hepatitis b virus associated with hepatocellular carcinoma: The present invention provides methods of predicting a pre-disposition of HBV-infected individuals to develop hepatacellular carcinoma (HCC)....

20060057564 - Identification and mapping of single nucleotide polymorphisms in the human genome: The invention relates to the role of genes in human diseases. More particularly, the invention relates to compositions and methods for identifying genes that are involved in human disease conditions. The invention provides identification and mapping of a very large number of SNPs throughout the entire human genome. This contribution...

20060057611 - Log-linear amplification: The present disclosure provides methods and composition to detect or quantitate one or more target sequences in a reaction that couples a linear amplification reaction to an exponential amplification reaction....

20060057622 - Low cycle amplification of rna molecules: The compositions and methods afford an almost unlimited linear RNA amplification from a few cells with minimal differences in the relative abundance of amplified RNAs and their parent mRNA (sample distortion)....

20060057597 - Method and system for processing nanoparticles using a self assembly mechanism to form combined species: A method for processing nanoparticles using a self assembly mechanism. The method includes flowing a first reactant species through a first channel region, which has a predetermined dimension including a first width and a first depth. The method includes flowing a second reactant species through a second channel region, which...

20060057567 - Method for constructing a chimeric dna library using a single strand speific dnase: The method for constructing a chimeric DNA library of the present invention uses matching regions of heterologous DNA sequences as internal primers and enables highly efficient DNA reassembly between heterologous DNAs having relatively low similarity. Therefore, the inventive method can be effectively used for developing a useful gene having better...

20060057593 - Method for detecting cystic fibrosis: The present invention relates to methods for amplifying various regions of the cystic fibrosis transmembrane regulator (CFTR) gene. Methods are provided for amplifying one or all 27 exons of the CFTR gene and a portion of the CFTR promoter region in a single tube. The method can identify the presence...

20060057569 - Method for detecting hybridization events in nucleic acids: The invention relates to materials and methods for detecting the presence of and/or quantifying a target polynucleotide in a sample by using probe polynucleotides attached to the surface of an array, which probes are at least partially complementary to the target polynucleotide. A kit for carrying out the invention is...

20060057613 - Method for distinguishing methicillin resistant s. aureus from methicillin sensitive s. aureus in a mixed culture: The present invention provides isolated oligonucleotides and methods for detecting a methicillin resistant Staphylococcus aureus in a sample, including a sample that comprises nucleic acid molecules of higher biological complexity than that of amplified nucleic acid molecules....

20060057604 - Method for electrically detecting oligo-nucleotides with nano-particles: A method for detecting a target oligo-nucleotides includes the following steps: providing a substrate mounted with at least a pair of detecting electrodes separated with a gap; coating a surface activation agent on the substrate; providing a plurality of nano-particles and immobilizing them between the two detecting electrodes on the...

20060057579 - Method for predicting a drug transport capability by abcg2 polymorphisms: The present invention relates to a polypeptide which excretes drugs such as cancer chemotherapeutic agents from a cell and to a gene coding therefor. More specifically, the present invention relates to a method for predicting a drug transport capability of a mammalian cell by determining a single nucleotide polymorphism(s) of...

20060057591 - Method for preventing viral infection: There is provided a method for preventing or treating viral infections using members of the annexin proteins family. In particular annexins 1 and 5 as well as inhibitors of heterotetrameric annexin 2, such as antibodies, significantly reduce the production of virus progeny....

20060057628 - Method for prognosticating the progress of breast cancer and compounds useful for prevention or treatment thereof: This invention concerns in vitro method for prognosticating the progress of breast cancer, comprising detecting or quantifying the level of 17β-hydroxysteroid dehydrogenase (17HSD) type 1 enzyme in breast tumor tissue sample, wherein the presence of said 17HSD type 1 enzyme is indicative of severe progress of breast cancer. Furthermore, the...

20060057575 - Method for two-dimensional conformation-dependent separation of non-circular nucleic acids: Methods are provided for separating linear nucleic acid fragments based on their conformation. The methods are based on novel two-dimensional gel electrophoresis techniques. In one aspect the method comprises electrophoresing a sample of nucleic acids in a first dimension said sample through a gel matrix under a first set of...

20060057568 - Method of identifying compounds that alter bag-1 mediated down-regulation of glucocorticoid-receptor transactivation: The present invention relates to new methods of identifying compounds that inhibit or reduce bag-1 mediated down-regulation of gluocorticoid-receptor (GR) tansactivation. These methods rely on the surprising findings, that first, a (poly)peptide comprising the motif X-Lys-Lys-Lys-Y-Arg-Arg-Arg also present is the cochaperone bag-1 is sufficient for DNA binding of this cochaperone...

20060057588 - Method of separating tumor cells with and without lymphotropic metastatic potential in a human carcinoma: A method for separating tumor cells with lymphotropic metastatic potential from those without lymphotropic metastatic potential in a human carcinoma. Cells of the carcinoma are transplanted in each of a plurality of fresh athymic mice. At least one of the athymic mice which does not develop a palpable tumor at...

20060057572 - Method of testing food: A method for detecting the presence/absence of a specific substance in foods by performing PCR using primers which are designed on the basis of data obtained from a part of a gene of the specific substance. This method is highly useful in detecting a trace component contained in a food...

20060057566 - Methods and compositions for analyzing nucleic acid molecules utilizing sizing techniques: Tags and linkers specifically designed for a wide variety of nucleic acid reactions are disclosed, which are suitable for a wide variety of nucleic acid reactions wherein separation of nucleic acid molecules based upon size is required....

20060057573 - Methods and reagents for detecting target binding by nucleic acid ligands: The present invention provides novel methods and reagents for detecting the binding of protein targets to nucleic acid ligands. Using Universal Protein Stains (UPS), proteins bound by nucleic acid ligands may be labeled with a detectable moiety. The methods and reagents are particularly useful for the detection of protein targets...

20060057600 - Methods for detecting dna viruses: A DNA virus in a sample is detected by contacting the sample with host cells that have been transiently transfected with a reporter sequence under the control of a virus-specific promoter, and then detecting the reporter....

20060057571 - Methods for detecting nucleic acids while using solid phase bound primers and cleaving the products of a cyclic amplification reaction (trap release primer amplification (tramp): The invention relates to methods for detecting nucleic acids with the aid of an amplification reaction whereby involving a novel structure of solid phase bound starter molecules (primers) with a cyclic amplification reaction on the solid phase and a transformation of the amplification products into the aqueous phase. The solid...

20060057612 - Methods for diagnosing osteoporosis or a susceptibility to osteoporosis based on haplotype association: Methods for diagnosis of osteoporosis or a susceptibility to osteoporosis based on detection of at risk haplotypes associated with BMP2 are disclosed....

20060057592 - Methods for identifying primase trinucleotide initiation sites and identification of inhibitors of primase activity: Methods and kits for the identification of a primase trinucleotide initiation site and for the identification of compounds which modulate bacterial primase activity are provided....

20060057609 - Methods for predicting methotrexate polyglutamate levels using pharmacogenetics: The present invention provides methods for determining a level of methotrexate polyglutamates (MTXPGs) in an individual undergoing methotrexate (MTX) therapy and for optimizing dose efficacy of MTX therapy in an individual by genotyping the individual at a polymorphic site in at least one folate pathway gene (e.g., a reduced folate...

20060057576 - Microcapillary hybridization chamber: The invention provides a microcapillay hybridization chamber made of a narrow bore tubing with probe segments. Each probe segment has oligonucleotide probes covalently attached to the inner wall of the tubing and the oligonucleotide probes within each segment have identical, known sequences. Many oligonucleotide probe segments can be present within...

20060057581 - Microfabricated fluidic device for fragmentation: A microfabricated device for fragmenting nucleic acids present in a fluid sample, the device comprising an inlet port (20), a fragmentation cell (1), and an outlet port (10) downstream from said inlet port (20), said cell (1) being in fluid communication with said ports, and wherein said outlet port is...

20060057630 - Mll translocations specify a distinct gene expression profile, distinguishing a unique leukemia: The present invention relates to the diagnosis of mixed lineage leukemia (MLL), acute lymphoblastic leukemia (ALL), and acute myelogenous leukemia (AML) according to the gene expression profile of a sample from an individual, as well as to methods of therapy and screening that utilize the genes identified herein as targets....

20060057619 - Mutant met and uses therefor: The present invention provides compositions and methods for detecting mutations in the proto-oncogene Met....

20060057631 - Nano-structured device for analysis or separation, and its preparation and application: This invention involves the nano-structured support used for separation or/and analysis, especially the chip substrate, ELISA plate substrate, planar chromatography strip and chromatography gel. Besides, it involves the functionalized nano-structured support of high sensibility for separation or/and analysis, especially the analysis-chip, ELISA plate, planar chromatography reagent strip and chromatography gel....

20060057585 - Nanostepper/sensor systems and methods of use thereof: Nanostepper/sensor systems and methods for analyzing a polymer are provided....

20060057583 - Novel compositions and methods for controlling the extendability of various components used in copying or amplification steps: This invention relates to novel compositions and methods for controlling the extendability of various components used in copying or amplification steps to provide an enhancement of the specificity of the nucleic acids produced by these processes as well as potentially increasing the amount of such products. By these means, the...

20060057587 - Oligonucleotide arrays comprising background probes: The invention relates to chemical arrays comprising a plurality of features comprising biopolymers at known locations on the substrate, wherein at least one feature comprises specific hybridization probes designed or selected to bind to specific target sequences and background probes for evaluating non-specific binding of target. Methods, systems, and kits...

20060057594 - Preconditioning for eda cell library: A characterized cell library for EDA tools includes one or more mathematical models for each cell, and one or more preconditioning functions (and/or inverse preconditioning functions) for each mathematical model. Each mathematical model represents a performance parameter (e.g., delay, power consumption, noise) or a preconditioned performance parameter of the cell....

20060057608 - Producing, cataloging and classifying sequence tags: The described method provides, methods, and kits to produce, identify, catalog and classify a comprehensive collection of nucleic acid targets produced from a nucleic acid sample. The method, referred to as Cataloging and Classification of Sequence Tags, involves generating a set of target nucleic acid fragments; coupling the target nucleic...

20060057606 - Reagents containing terminal-phosphate-labeled nucleotides for nucleic acid sequencing: The present invention is directed to a method of sequencing a target nucleic acid molecule having a plurality of bases. In its principle, the temporal order of base additions during the polymerization reaction is measured on a molecule of nucleic acid, i.e. the activity of a nucleic acid polymerizing enzyme...

20060057615 - Risk prediction for hypertension, elevated plasma triglyceride and metabolic syndrome: The lipoprotein lipase (LPL) gene is associated with hypertension, elevated plasma triglyceride and metabolic syndrome. A haplotype in the intron 3-intron 4 region (the C/AA/T haplotype), and a haplotype in the intron 5-intron 6 region (the GTAG haplotype), are particularly useful in predicting the risk of an individual for these...

20060057590 - Rna probes: The invention is based in part on the generation of a double stranded RNA molecule substantially covering the whole transcribed region of a gene, and cleaving this using an RNA endonuclease to generate small RNA molecules which are already or may be subsequently labelled. The invention provides small labelled ribonucleic...

20060057602 - Rtvp based compositions and methods for the treatment of prostate cancer: This invention relates to a gene encoding RTVP that has been shown to be up-regulated by p53 using differential display-PCR and subsequently by co-transfection studies. RTVP-1 mRNA is abundant in normal mouse and human prostatic epithelial cells and primary tumors, but is significantly down regulated in metastatic mouse and human...

20060057625 - Scaffold-based artificial receptors and methods: The present invention relates to scaffold artificial receptors, methods of and compositions for making them, and methods of using them. Each artificial receptor includes a plurality of building blocks. The plurality of the building blocks are coupled to a scaffold....

20060057627 - Selection scheme for enzymatic function: The present invention includes compositions and methods of selecting functional genetic elements that includes selecting in vivo an autogene operon with one or more mutagenized functional genetic elements and one or more selectable elements, wherein survival of the autogene depends on expression of the one or more mutagenized functional genetic...

20060057623 - Senp1 as a marker of cancer development and target for cancer therapy: The present invention relates to compositions and methods for the use of SENP1 as a marker for cancer diagnosis, specifically prostate cancer. Still further, it relates to the use of inhibitors of SENP1 to inhibit cell proliferation of a neoplasm or tumor cell and to treat a hyperproliferative disease....

20060057616 - Sensitive detection of bacteria by improved nested polymerase chain reaction targeting the 16s ribosomal rna gene and identification of bacterial species by amplicon sequencing: A method for identifying an RNA form of a bacteria, comprising reverse transcribing RNA material; conducting PCR using primers for a first highly conserved genetic sequence generic of the bacteria; conducting nested PCR using primers for a second highly conserved genetic sequence within the first genetic sequence of the bacteria;...

20060057607 - Small rnas and bacterial strains involved in quorum sensing: Quorum-sensing bacteria communicate with extracellular signal molecules called autoinducers to allow community-wide synchronization of gene expression. The present invention relates to the identification the Vibrio harveyi and Vibrio cholerae protein Hfq as mediating interactions between small, regulatory RNAs (sRNAs) and specific messenger RNA (mRNA) targets. Accordingly, the present invention provides...

20060057599 - System for autonomous monitoring of bioagents: An autonomous monitoring apparatus for monitoring air, water, soil, or other substance for bioagents. A collector gathers a quantity of the air, water, soil, or other substance being monitored. A sample preparation system prepares a sample of the selected potential bioagent particles. The sample is analyzed by a system for...

20060057610 - System for producing synthetic promoters: The present application discloses a method for making and selecting a transcription enhancing combined promoter cassette, which includes constructing a library of randomly combined transcription regulatory elements, which comprise double stranded DNA sequence elements that are recognized by transcription regulators; inserting the combined transcription regulatory elements upstream of a minimum...

20060057614 - Tethering neuropeptides and toxins for modulation of ion channels and receptors: The present invention provides a method for tethering peptides, including neuropeptides and peptide toxins, including naturally occurring and mutant or altered peptides, to a surface, including the cell surface. The tethered peptides are not naturally tethered or attached to a surface, including a cell surface, or through a cell membrane....

20060057574 - Tissue specific expression: The present invention relates to a synthetic regulatory element for tissue specific gene expression. Furthermore, the invention relates to a tumor cell specific expression system for the expression of therapeutic genes as well as a pharmacological composition....

20060057586 - Transcript mapping method: A transcript mapping method according to an embodiment of the invention is described hereinafter and combines short tag based (SAGE and MPSS) efficiency with the accuracy of full-length cDNA (flcDNA) for comprehensive characterization of transcriptomes. This method is also referred to as Gene Identification Signature (GIS) analysis. In this method,...

20060057620 - Treatment of hiv infection: A method