Chemistry: molecular biology and microbiology inventions 01/06

20060019233 - Delivery of high cell mass in a syringe and related methods of cryopreserving cells: This invention relates to methods and apparatus for cryopreserving biological materials for extended periods of time. In an exemplary embodiment, the method comprises suspending biological materials in a cryosolution, freezing the biological materials in the apparatus, and removing the frozen biological materials from the apparatus to thaw them for use....

20060019234 - Modern blood banking employing improved cell preservation composition: An improved anticoagulant is based on a higher level of citric acid than is usual (at least about 0.2% weight by volume). The higher citrate is combined with an amino acid as a counterion. The amino acid prevents cellular damage often caused by elevated citrate levels. The amino acid citrate...

20060019237 - In vitro wound healing assay and device: Devices for creating model wounds in vitro are provided, along with kits and systems including the devices. Methods for making such devices and for modeling wounds are also provided....

20060019236 - Membrane fusion assay: The present invention relates to an assay for detecting membrane fusion. More particularly the present invention relates to assays for detecting exocytosis and/or endocytosis wherein membrane fusion is detected by an increase in fluorescence of a lipophilic fluorescent dye due to fluorescence resonance energy transfer (FRET) occurring from one lipophilic...

20060019235 - Molecular and functional profiling using a cellular microarray: Cells are profiled with respect to their expression of cell surface molecules, and ability to respond to external stimulus in the microenvironment. External stimuli include cell-cell interactions, response to factors, and the like. The cells are arrayed on a planar or three-dimensional substrate through binding to immobilized or partially diffused...

20060019242 - Detection of herpes simplex virus: The invention provides methods to detect herpes simplex virus (HSV) in biological samples and further to distinguish between HSV-1 and HSV-2. Primers and probes for the differential detection of HSV-1 and HSV-2 are provided by the invention. Articles of manufacture containing such primers and probes for detecting HSV are further...

20060019245 - Hcv variants: HCV variants are described. The variants include polynucleotides comprising non-naturally occurring HCV sequences and HCV variants that have a transfection efficiency and ability to survive subpassage greater than HCV that have wild-type polyprotein coding regions. Expression vectors comprising the above polynucleotides and HCV variants are also described, as are the...

20060019243 - Method and device for mixing samples on a support: A method for detecting analytes in a liquid is provided in which the liquid is subjected to a mixing treatment on an area of a support which has in particular immobilized reactants, wherein in the mixing treatment the liquid is impinged upon by a stream of gas that sweeps across...

20060019238 - Method for identifying protein-protein interactions: The properties of yeast help, a type I ER membrane protein which is involved in the unfolded protein response (UPR), have been exploited to develop â. system for the detection and study of interactions between extracellular and/or membrane proteins. In the system, proteins of interest are fused to the lumenal...

20060019239 - Method of preventing infections from bioterrorism agents with immunostimulatory cpg oligonucleotides: The present disclosure relates to a method of preventing or treating an infection caused by a bioterrorism agent, specifically to a method of increasing an immune response to a bioterrorism agent using an oligodeoxynucleotide including a CpG motif, and a method of enhancing the immunogenicity of a vaccine against a...

20060019241 - Na+ and ci-coupled transport system for endogenous opioid peptides: The present invention provides the identification and characterization of an endogenous opioid peptide transporter, and uses thereof....

20060019244 - Planar optical waveguide based sandwich assay sensors and processes for the detection of biological targets including protein markers, pathogens and cellular debris: An assay element is described including recognition ligands bound to a film on a single mode planar optical waveguide, the film from the group of a membrane, a polymerized bilayer membrane, and a self-assembled monolayer containing polyethylene glycol or polypropylene glycol groups therein and an assay process for detecting the...

20060019240 - Posh nucleic acids, polypeptides and related methods: The application discloses novel polypeptides and nucleic acids involved in a variety of biological processes, including viral reproduction. Related methods and compositions are also described....

20060019246 - Synthetic c-glycolipid and its use for treating cancer, infectious diseases, and autoimmune diseases: wherein X is O or NH; R′ is a hydrocarbon chain; R3 and R4 are hydrogen, OH or a monosaccharide; R5 is hydrogen or a monosaccharide; Q′ is optionally present and may be a C1-10 hydrocarbon; X′ is optionally present and may be O, S or NR8; and Q3 may...

20060019262 - Activation induced deaminase (aid): The present invention identifies that the expression of Activation Induced Deaminase (AID) or its homologues in cells confers a mutator phenotype and thus provides a method for generating diversity in a gene or gene product as well as cell lines capable of generating diversity in defined gene products. The invention...

20060019253 - Amplicon melting analysis with saturation dyes: Methods are provided for nucleic acid analysis wherein a target nucleic acid that is at least partially double stranded is mixed with a dsDNA binding dye having a percent saturation of at least 50% to form a mixture. In one embodiment, the nucleic acid is amplified in the presence of...

20060019285 - Analysis of intracellular modifications: Improved methods of determining the intracellular state of a protein as well as modifications of the protein are provided by introducing a surrogate fusion protein comprising a member of an enzyme fragment complementation complex and a target protein. After exposing cells transformed with the surrogate fusion protein to a change...

20060019299 - Bio-molecules detecting apparatus using electromagnetic induction and detecting method using the same: Provided are a bio-molecules detecting apparatus using electromagnetic induction and a detecting method using the same. An exemplary apparatus includes: a cantilever of which one end is fixed and the other end is set up to be movable; a first metal formed on a plane of the cantilever and receiving...

20060019259 - Characterization of biopolymers by resonance tunneling and fluorescence quenching: The present invention provides a method and apparatus for determining the identity of a monomeric residue of a biopolymer. The apparatus comprises a substrate having a nanopore, a potential-producing element for producing a ramped potential across electrodes adjacent to the nanopore, and a quenchable excitable moiety adjacent to the nanopore....

20060019291 - Cloning system for construction of recombinant expression vectors: Cloning systems are provided for constructing expression vectors. In one aspect of the invention, a kit is provided for constructing one or more recombinant expression vectors. The kit comprises: a linear driver DNA comprising a promoter sequence, a donor recombination site, and at least one selectable marker, the linear driver...

20060019283 - Common marmoset-derived hprt gene and use thereof: The present invention provides a common marmoset-derived hypoxanthine-guanine phosphoribosyltransferase gene and use thereof....

20060019289 - Compositions and methods for gene expression analysis: The present disclosure provides methods and composition to detect or quantitate one or more target sequences....

20060019257 - Compositions and methods for increasing protein production: Compositions and methods for increasing protein production are provided....

20060019256 - Compositions and methods for treating and diagnosing cancer: The present invention relates to compositions and methods for treating, characterizing, and diagnosing cancer. In particular, the present invention provides gene expression profiles associated with solid tumor stem cells, as well as novel stem cell cancer markers useful for the diagnosis, characterization, and treatment of solid tumor stem cells....

20060019273 - Detection card for analyzing a sample for a target nucleic acid molecule, and uses thereof: The present invention relates to a detection card made of a first planar element having a detection chip. The detection chip has two or more electrically separated conductors fabricated on a substrate and capture probes attached to the conductors, such that a gap exists between the capture probes on the...

20060019306 - Detection of ochratoxin a producing fungi: Nucleotide or amino acid sequences that may be used in the detection and/or identification fo an ochratoxigenic fungus or in the construction of an atoxigenic strain of an ochratoxigenis fungus. The fungus may be of the genus Aspergillus, species carbonarius, niger, alliaceus, or foetidux. The fungus may also be of...

20060019305 - Diagnosis and treatment of ptp related disorders: The present invention relates to PTP05 polypeptides and PTP10 polypeptides, nucleic acids encoding such polypeptides, cells, tissues and animals containing such nucleic acids, antibodies to such polypeptides, assays utilizing such polypeptides, and methods relating to all of the foregoing. Methods for treatment, diagnosis, and screening are provided for PTP05 and/or...

20060019272 - Diagnosis of disease and monitoring of therapy using gene expression analysis of peripheral blood cells: Disclosed are methods to diagnose a patient that has a pulmonary disease, and particularly, pulmonary arterial hypertension, using biomarkers that are differentially regulated in the peripheral blood cells of patients with such disease as compared to individuals that do not have the disease. Also disclosed are methods to diagnose a...

20060019249 - Diagnostic conjugate useful for intracellular imaging and for differentiating between tumor-and non-tumor cells: Described is a diagnostic conjugate comprising (a) a transmembrane module (TPU), (b) an address module (AS), preferably an antisense peptide nucleic acid (PNA), and (c) a signalling module (SM). Said conjugate is useful for intracellular imaging, preferably via MRI, and, e.g., for differentiating between tumor- and non-tumor cells....

20060019277 - Digital amplification for detection of mismatch repair deficient tumor cells: The detection of mutations in fecal DNA represents a promising, non-invasive approach for detecting colorectal cancers in average risk populations. One of the first practical applications of this technology involves the examination of microsatellite markers to sporadic cancers with mismatch repair deficiencies. As such cancers nearly always occur in the...

20060019252 - Genes and polypeptides relating to hepatocellular or colorectal carcinoma: The present application provides novel human genes WDRPUH and KRZFPUH and PPIL1 whose expression is markedly elevated in a great majority of HCCs and colorectal cancers, respectively, compared to corresponding non-cancerous tissues, as well as novel human gene APCDD1 whose expression is elevated in primary colon cancers and down-regulated in...

20060019295 - Genomic barcoding for organism identification: The invention disclosed herein relates to the comparison of whole genomes to identify short oligonucleotide sequences that are specific to a single organism. In some embodiments of the invention, combinations of species-specific oligonucleotides are used to produce specific amplification products. In some embodiments, isolate-specific oligonucleotides are used to detect and...

20060019270 - Global dna methylation assessment using bisulfite pcr: The present invention provides a method and assay for determining global methylation using the methylation status of repetitive DNA elements as a surrogate marker. Also provided by the invention is a method for determining efficacy of a DNA methylation inhibiting drug by using changes in repetitive DNA methylation as a...

20060019297 - Glyceraldehyde-3-phosphate dehydrogenase and phosphoglycerate mutase regulatory sequences for gene expression in oleaginous yeast: The regulatory sequences associated with the Yarrowia lipolytica glyceraldehyde-3-phosphate dehydrogenase (gpd) and phosphoglycerate mutase (gpm) genes have been found to be particularly effective for the expression of heterologous genes in oleaginous yeast. The promoter regions of the invention, intron and enhancer have been shown to drive high-level expression of genes...

20060019286 - High throughput methods relating to microrna expression analysis: The invention relates to methods and compositions for microRNA expression analysis using microarrays....

20060019290 - Identification of markers in esophageal cancer, colon cancer, head and neck cancer, and melanoma: Methods for identifying expression of markers indicative of the presence of esophageal, a squamous cell cancer, a squamous cell cancer of the head and neck, colon cancer and melanoma are provided. Also provided are articles of manufacture useful in such methods and compositions containing primers and probes useful in such...

20060019284 - Identification of polynucleotides for predicting activity of compounds that interact with and/or modulate protein tyrosine kinases and/or protein tyrosine kinase pathways in lung cancer cells: The present invention describes polynucleotides that have been discovered to correlate to the relative intrinsic sensitivity or resistance of cells, e.g., lung cell lines, to treatment with compounds that interact with and modulate, e.g., inhibit, protein tyrosine kinases, such as, for example, members of the Src family of tyrosine kinases,...

20060019278 - Marker for prenatal diagnosis and monitoring: The present invention relates to new methods for diagnosing a pregnancy-associated disorder by analyzing fetal DNA present in the mother's blood. More specifically, this invention relies on the discovery that the maspin gene is differentially methylated in fetal DNA and in maternal DNA and provides these new diagnostic methods, which...

20060019302 - Method and apparatus for applying fluids to a biological sample: The invention is directed to a method of contacting a biological sample with a solution, comprising the steps of moving a curved surface wetted with the solution in proximity to the biological sample whereby the distance separating the wetted curved surface and the biological sample is sufficient to form a...

20060019247 - Method and apparatus for nucleic acid sequencing and identification: The methods and apparatus 100 disclosed herein are of use for sequencing and/or identifying nucleic acids 230, 310. Nucleic acids 230, 310 containing labeled nucleotides 235, 245, 315 may be synthesized and passed through nanopores 255, 310. Detectors 257, 345 operably coupled to the nanopores 255, 310 may detect the...

20060019282 - Method and unit for detecting an interaction such as hybridization, bioassay plate provided with a number of such detecting units, system for detecting an interaction such as hybridization, and reagent kit: Disclosed is a method for the detection of an interaction between substances. According to the method, a salt-containing HEPES buffer is allowed to exist in a reaction region that provides a place of interaction for the interaction. The concentration of the salt in the salt-containing HEPES buffer may be adjusted...

20060019300 - Method for in vitro molecular evolution of protein function: The invention provides a method for generating a polynucleotide sequence or population of sequences from parent single stranded polynucleotide sequences encoding one or more protein motifs, comprising the steps of a) providing single stranded DNA constituting plus and minus strands of parent polynucleotide sequences; b) digesting the single stranded polynucleotide...

20060019264 - Method for isolation of independent, parallel chemical micro-reactions using a porous filter: The present invention relates to methods and apparati for conducting densely packed, independent chemical reactions in parallel in a substantially two-dimensional array. Accordingly, this invention also focuses on the use of this array for applications such as DNA sequencing, most preferably pyrosequencing, and DNA amplification....

20060019260 - Method for tapping the immunological repertoire: The present invention relates to a method for isolating from the immunological gene repertoire a gene coding for a receptor having the ability to bind a preselected ligand. Receptors produced by the gene isolated by the method, particularly catalytic receptors, are also contemplated....

20060019250 - Method for the detection of carcinoembryonic agent and cancer diagnosis method based thereon: The present invention provides a method of detecting carcinoembryonic antigen (CEA) comprising detecting a DNA sequence comprising the sequence: GATATAGCAGCCCTGGTGTAGTTTCTTCATTTCAGGAAGACTG. The invention also provides a method of diagnosis of disseminated cancer comprising obtaining a cell sample, extracting DNA from the cell and detecting the presence of the sequence: GATATAGCAGCCCTGGTTSGTTCTTCATTTCAGGAAGACTG....

20060019251 - Method for the qualitative and quantitative determination of genetic material: The invention relates to a method for qualitatively and quantitatively determining genetic material which contains a known target sequence which is characteristic for the material and which can be present alongside, or in a mixture with, other genetic material, in which a suspension which contains the genetic material possessing the...

20060019303 - Method to identify and analyze genes having modified expression in stimulated t cells: A method of identifying genes involved in the stimulation of primary T cells comprising the steps of: a) contacting experimental cells with a stimulating agent; b) preparing RNA from said experimental cells at one or more stimulation phases; c) measuring the level of gene expression in the cells; d) comparing...

20060019263 - Methods and apparatuses for analyzing polynucleotide sequences: Methods for high speed, high throughput analysis of polynucleotide sequences, and apparatuses with which to carry out the methods are provided in the invention....

20060019258 - Methods and compositions for detection of small interfering rna and micro-rna: The invention provides a method of distinguishing small RNA from mRNA by contacting a biological isolate with a phosphate reactive reagent having a label moiety under conditions wherein the label moiety is preferentially added to the 5′ phosphate of small RNA over the 5′ cap structure of mRNA and distinguishing...

20060019276 - Methods and devices for nucleic acid sequence determination: Methods of the invention comprise methods and devices for nucleic acid sequence determination. Generally, the invention relates to preparing a substrate for sequencing a target nucleic acid....

20060019267 - Methods and kits for analyzing polynucleotide sequences: The present invention features methods for analyzing a sequence of a target polynucleotide by detecting incorporation of a nucleotide into its complementary strand, where the polynucleotides may be bound at high density and at single molecule resolution. The invention also features labeling moieties and blocking moieties, which facilitate chain termination...

20060019279 - Methods for target molecule detection using siderophores and related compositions: The invention provides methods for isolating a target molecule from a sample. In an embodiment, the method involves contacting a sample with a capture agent, the agent comprising a siderophore and a transition metal cation, under conditions wherein the agent is capable of binding a target molecule to form a...

20060019296 - Methods of indentifying modulators of the fgf receptor: The present invention provides fragments of SNT and FGFR which can form a binding complex that is amenable to structural determinations by NMR spectroscopy. The three-dimensional structural data is also included as part of the invention. In addition, the present invention provides methodology for related structure based rational drug design...

20060019301 - Methods of producing mutant polynucleotides: The present invention relates to methods of producing mutants of a polynucleotide and to mutant polynucleotides and artificial variants encoded by the mutant polynucleotides....

20060019288 - Methods, reaction mixtures, and kits for ligating polynucleotides: The present teachings pertain to methods, reaction mixtures, and kits for ligating polynucleotides. In some embodiments, a heat-activatable ligation agent, a phosphorylation agent, and a decontamination agent are included in the same ligation reaction mixture with at least one probe set, at least one linker set, and at least one...

20060019268 - Molecular markers of cisplatin resistance in cancer and uses thereof: The present invention relates generally to the fields of molecular biology and medicine. More particularly, it concerns cancer treatments and the identification and use of markers for cancer resistance to a platinum-drug based therapy therapy. In certain embodiments, the present invention provides diagnostic and/or prognostic methods involving a collection of...

20060019248 - Mutant sh-3 binding protein compositions and methods: The invention provides mutant SH3-binding protein (SH3BP2) nucleic acids, polypeptides, and agents which selectively bind to the mutant SH3BP2 molecules and which do not bind to the wild type SH3BP2 molecules. Methods for selecting agents which inhibit mutant SH3BP2 expression, as well as diagnostic and therapeutic methods which utilize the...

20060019280 - Mutations in kit confer imatinib resistance in gastrointestinal stromal tumors: The present invention relates to methods and compositions concerning resistance to a drug for cancer comprising aberrant KIT signal, such as aberrant KIT sequence or expression. In a specific embodiment, the cancer is also initially responsive to imatinib therapy, such as in gastrointestinal stromal tumors (GISTs). In particular embodiments, a...

20060019293 - Mutations of the 5' region of the human 5-ht1a gene, associated proteins of the 5' region and a diagnostic test for major depression and related mental illnesses: Clinical response to antidepressant compounds correlates with a selective down-regulation of presynaptic 5-HT1A receptors in serotonergic raphe neurons. Thus regulation of the 5-HT1A receptor gene could play a crucial role in the treatment or etiology of major depression. The promoter and repressor activities of the human 5-HT1A receptor gene have...

20060019274 - Nano-pcr: methods and devices for nucleic acid amplification and detection: Methods, devices, and compositions are described that provide for amplification of nucleic acid sequences without reliance upon temperature cycling, thus freeing the methods from conventional benchtop thermal cycling devices. Denaturation of double stranded nucleic acids, primer annealing, and precision control over primer extension by polymerase can be accomplished by applying...

20060019266 - Novel nucleotide mixture for improved nucleic acid amplification performance: The present invention relates to modification of amplification buffer used in amplification reactions. The modifications result in a significant improvement in results of amplification In particular, the present invention provides methods and buffers for performing an amplification reaction utilizing a buffer comprising nucleotide triphosphates comprising treating the buffer to substitute...

20060019275 - Nucleic acids encoding proteins involved in sensory transduction: The invention provides isolated nucleic acid and amino acid sequences of sensory cell specific polypeptides, antibodies to such polypeptides, methods of detecting such nucleic acids and polypeptides, and methods of screening for modulators of sensory cell specific polypeptides....

20060019255 - Nucleotide sequences specific to brucella and methods for the detection of brucella: Described herein are the identification of nucleotide sequences specific to Brucella that serves as a marker or signature for identification of this bacterium. In addition, forward and reverse primers and hybridization probes derived from these nucleotide sequences that are used in nucleotide detection methods to detect the presence of the...

20060019254 - Nucleotide sequences specific to yersinia pestis and methods for the detection of yersinia pestis: Described herein are the identification of nucleotide sequences specific to Yersinia pestis that serves as a marker or signature for identification of this bacterium. In addition, forward and reverse primers and hybridization probes derived from these nucleotide sequences that are used in nucleotide detection methods to detect the presence of...

20060019261 - Rna-binding protein: The present invention provides Synaptotagmin-binding and yet RNA-binding proteins and genes encoding the proteins. The invention relates to a recombinant protein selected from the group consisting of the following (a) and (b), as well as a gene encoding the protein: (a) a protein comprising the amino acid sequence as shown...

20060019287 - Secreted and transmembrane polypeptides and nucleic acids encoding the same: The present invention is directed to novel polypeptides and to nucleic acid molecules encoding those polypeptides. Also provided herein are vectors and host cells comprising those nucleic acid sequences, chimeric polypeptide molecules comprising the polypeptides of the present invention fused to heterologous polypeptide sequences, antibodies which bind to the polypeptides...

20060019292 - Sequence specific recombinase-based methods for producing intron containing vectors and compositions for use in practicing the same: Methods are provided for producing a vector that includes at least one splicable intron. In the subject methods, intron containing vectors are produced from donor and acceptor vectors that each include a site specific recombinase site, where the subject donor and acceptor vectors further include splice donor and acceptor sites...

20060019298 - Sequencing of modified nucleic acid molecules: Modified nucleic acids are used for many purposes in research, diagnostic, and treatment protocols. However, direct sequencing of such molecules is generally considered to require special conditions. Methods of making a cDNA using a modified nucleic acid molecule and sequencing methods of sequencing a modified nucleic acid molecule are described....

20060019304 - Simultaneous analysis of multiple genomes: The invention provides methods for multiplexing readouts from multiple hybridization-based assays that each comprise one or more hybridization or annealing steps and one or more enzymatic processing steps. In one aspect, the invention permits simultaneously analysis of a plurality of genomes by separately hybridizing a set of probes with the...

20060019271 - Submergence-induced protein-like factors: A pure polypeptide containing an amino acid sequence at least 80% identical to SEQ ID NO:5. The polypeptide, when expressed in a cell, increases susceptibility of the cell to hepatitis C virus infection....

20060019269 - Susceptibility gene for myocardial infarction, stroke, and paod, methods of treatment: Linkage of myocardial infarction (MI) and a locus on chromosome 13q12 is disclosed. In particular, the FLAP gene within this locus is shown by genetic association analysis to be a susceptibility gene for MI and ACS, as well as stroke and PAOD. Pathway targeting for treatment and diagnostic applications in...

20060019265 - Transmission-based luminescent detection systems: A luminescent detection system that employs transmission-based detection is provided for use with a chromatographic-based assay device. Unlike conventional systems, the detection system of the present invention is portable, simple to use, and inexpensive. For example, the system may be selectively controlled to reduce reliance on expensive optical components, such...

20060019281 - Tumor suppressor car-1: The present invention relates to a new tumor suppressor, designated CAR-1, the gene for which is located on the short arm of human chromosome 1. This gene is directly implicated in colon, kidney and breast cancers, and the CAR-1 ubiquitous expression of the corresponding transcript suggests that it may be...

20060019294 - Tyrosine kinase substrate (tks) proteins: The present invention relates to novel Tks 107, Tks 113, Tks 118 and Tks 202 polypeptides, nucleotide sequences encoding polypeptides, as well as various products and methods useful for the diagnosis and treatment of various related diseases and conditions....

20060019316 - Apparatus and method for automated protein design: The present invention relates to apparatus and methods for quantitative protein design and optimization....

20060019308 - Cd38 modulated chemotaxis: The present invention relates to methods for modulating the migratory activity of cells expressing CD38 for the treatment of disorders including, but not limited to, inflammation, ischemia, asthma, autoimmune disease, diabetes, arthritis, allergies, infection with pathogenic organisms, such as parasites, and transplant rejection. Such cells include, for example, neutrophils, lymphocytes,...

20060019310 - Cell regulatory genes, encoded products, and uses related thereto: Disclosed herein are methods for diagnosing cancer in a biological sample comprising measuring the level of a p63 gene product in the sample....

20060019312 - Cis-interactions between mhc molecules and co-expressed proteins, related neurological and non-neurological disorders and related therapeutic/diagnostic utilities: The present invention relates to the interactions between MHC Class I molecules and Co-expressed Proteins within the same cell (i.e., cis interaction). These interactions affect overt functional cellular processes by way of influencing protein trafficking and/or signal transduction. For example, in the nervous system, MHC Class I/Co-expressed Protein Interactions can...

20060019314 - Increasing life span by modulation of smek: The Smek (Suppressor of mek null) gene is described and characterized. Smek acts in the stress response pathway of animals by binding to and enhancing the transcription of FOXO, thereby providing the link between the stress response pathway and the insulin/IGF-1 pathway. Given the link between both the stress response...

20060019307 - Interleukin-8 homologous polypeptides and therapeutic uses thereof: The present invention is directed to novel polypeptides having structural homology to IL-8 and to nucleic acid molecules encoding those polypeptides. Also provided herein are vectors and host cells comprising those nucleic acid sequences, chimeric polypeptide molecules comprising the polypeptides of the present invention fused to heterologous polypeptide sequences, antibodies...

20060019313 - Method for detecting molecular surface interactions: A method for determining interaction of an analyte with a binding agent immobilized to a solid support surface by contacting the surface with a fluid sample containing the analyte, and detecting binding events at the solid support surface, wherein the sample is based on a complex medium containing at least...

20060019315 - Multimarker panel for diabetes type 1 and 2: The present invention relates to a method and means for diagnosing or risk stratification of co-morbidities, particularly cardiovascular complications in diabetes patients. The invention particularly relates to a method for diagnosing whether a diabetes patient is suffering from a cardiovascular complication or is at risk of suffering from a cardiovascular...

20060019311 - Process for detecting prp using a macrocyclic adjuvant ligand: A process for detecting the forms of the prion pathogens responsible for subacute, transmissible, spongiform encephalopathies, including a macrocyclic adjuvant ligand (AML), free or bound to a support, that is added to a biological sample capable of containing PrPsc, the resulting suspension then being reacted with an anti-PrPsc antibody, and...

20060019309 - Self-assembling peptide surfaces for cell patterning and interactions: This invention describes self assembled monolayers (SAMs) manufactured by imprinting reactive peptides onto solid supports. The invention further relates to methods of preparing and using these improved SAMs....

20060019317 - Assay systems and methods for detecting molecules that interact with membrane channels: The invention provides an assay system for detecting a molecule that interacts with a membrane channel, the assay system including cell membranes including one or more membrane channels; support bodies including scintillant and a coupling agent that associates with the cell membrane; a ligand that is selected to bind the...

20060019318 - Conformational and topological protein regulation: Methods and compositions are provided for identifying novel conformers of proteins not known to exist as different conformers. By using chimeric genes replacing a native signal sequence with a different signal sequence resulting in the production of a conformer, one can compare the native protein with the product of the...

20060019319 - Methods and apparatuses for conducting assays: Disclosed are methods for conducting assays of samples, such as whole blood, that may contain cells or other particulate matter. Also disclosed are systems, devices, equipment, kits and reagents for use in such methods. One advantage of certain disclosed methods and systems is the ability to rapidly measure the concentration...

20060019322 - Compositions and methods relating to prostate specific genes and proteins: The present invention relates to newly identified nucleic acids and polypeptides present in normal and neoplastic prostate cells, including fragments, variants and derivatives of the nucleic acids and polypeptides. The present invention also relates to antibodies to the polypeptides of the invention, as well as agonists and antagonists of the...

20060019321 - Planar optical waveguide based sandwich assay sensors and processes for the detection of biological targets including early detection of cancers: An assay element is described including recognition ligands adapted for binding to carcinoembryonic antigen (CEA) bound to a film on a single mode planar optical waveguide, the film from the group of a membrane, a polymerized bilayer membrane, and a self-assembled monolayer containing polyethylene glycol or polypropylene glycol groups therein...

20060019320 - Wnt mediated erbb signalling, compositions and uses related thereto: The present invention makes available assays and reagents inhibiting ErbB signals produced by Wnt protein comprising contacting a cell expressing ErbB and Wnt receptor (Frizzled) with a Wnt antagonist in a sufficient amount to reduce the sensitivity of the cell to ErbB signaling....

20060019324 - Pknb kinase and pstp phosphatase and methods of identifying inhibitory substances: The present invention relates to a pknB kinase and a pstP phosphatase as well as their use for identifying antibacterial substances....

20060019323 - Recombinant adenylate cyclase of bordetella sp. for diagnostic and immunomonitoring uses, method of diagnosing or immunomonitoring using said recombinant adenylate cyclase, and kit for diagnosing or immunomonitoring comprising said recombinant adenylate c: Diagnostic testing and immunomonitoring that uses genetically detoxified Bordetella pertussis CyaA as a delivery system are effective in tracking any immune responses, such as those generated by infectious and non-infectious diseases, or vaccinations, for example. T cells previously stimulated by a given antigen can be restimulated in vitro by the...

20060019325 - Screening assay: Screening assays for identifying an agent that modulates the activity of a phosphate catalyzing enzyme....

20060019326 - Use of three-dimensional microfabricated tissue engineered systems for pharmacologic applications: The present invention generally relates to a combination of the fields of tissue engineering, drug discovery and drug development. It more specifically provides new methods and materials for testing the efficacy and safety of experimental drugs, defining the metabolic pathways of experimental drugs and characterizing the properties (e.g., side effects,...

20060019327 - Transcutaneous analyte sensor: e

20060019328 - Glucose dehydrogenase: Modified water-soluble glucose dehydrogenases having pyrrolo-quinoline quinone as a coenzyme are provided wherein at least one amino acid residue is replaced by another amino acid residue in a specific region. Modified water-soluble PQQGDHs of the present invention have improved affinity for glucose....

20060019329 - Method of screening for protein inhibitors and activators: Chemical agents which are inhibitors or activators of a protein whose expression affects a phenotypic characteristic of the cell, especially a cultural or morphological characteristic, can be identified by their more pronounced effect on cells producing higher, usually non-naturally occurring, levels of the protein, than on cells producing little or...

20060019331 - Method and system for generating a telephone alert indicating the presence of an analyte: A system is provided for detecting a presence or amount of an analyte in a sample. The system may include a detection device detecting a characteristic of a fluid in communication with the sample to generate an electrical signal corresponding to the characteristic of the fluid and a processor coupled...

20060019330 - Staphylococcus detection: The invention relates to methods of detecting staphylococcus....

20060019332 - Deparaffinization compositions for dewaxing tissue specimens: Compositions and methods are provided for dewaxing wax-embedded biological specimens prior to histochemical analysis. The compositions and methods provided can effectively remove wax or improved wax-based embedding materials, particularly paraffin-based, from specimens during preparation for histochemical or other diagnostic analyses, while minimizing danger to users, achieving compatibility with automated use,...

20060019333 - Control of reactor environmental conditions: The present disclosure generally relates to chemical, biological, and/or biochemical reactor chips and/or reaction systems such as microreactor systems, as well as methods for constructing and using such systems. In some cases, humidity control materials are utilized to provide beneficially high rates of gas exchange. The humidity control materials may...

20060019334 - Production of flavonoids by recombinant microorganisms: Methods and compositions are provided for production of flavonoids in microbial hosts. The compositions comprises a set of genes which encode for enzymes involved in one or more steps in the biosymthetic pathway for the conversion of phenylpropanoids to various flavonoids. The method comprises the steps of introducing the set...

20060019341 - Aminopeptidase: A gram-negative bacterial cell is described that is deficient in a chromosomal gene present in a wild-type such cell which gene shares at least 80% sequence identity with the native sequence of the yfcK gene and encodes an aminopeptidase. Alternatively, a gram-negative bacterial cell is deficient in a chromosomal gene...

20060019347 - Biosynthetic polypeptides utilizing non-naturally encoded amino acids: Modified biosynthetic polypeptide molecules, methods for manufacturing, and uses thereof are provided....

20060019344 - C-erbb-2 external domain: gp75: Disclosed are methods and compositions for identifying malignant tumors that overexpress the c-erbB-2 oncogene. Assays useful for diagnosis and prognosis of neoplastic disease are provided which detect the external domain of c-erbB-2, the glycoprotein gp75 and quantitate the level of gp75 in the biological fluids of mammals carrying a tumor...

20060019340 - Cd44 polypeptides, polynucleotides encoding same, antibodies directed thereagainst and method of using same for diagnosing and treating inflammatory diseases: An isolated polypeptide is provided. The isolated polypeptide comprising an antigen recognition domain capable of specifically binding a CD44 polypeptide as set forth in SEQ ID NO: 2 and incapable of binding a CD44 polypeptide selected from the group consisting of: SEQ ID NO: 4 or 6....

20060019335 - Compositions and methods to promote c-iap autodegradation: The present invention generally relates to a method that may be employed as a means to regulate apoptosis. More particularly, the current invention relates to the use of Smac nucleic acid sequences and amino acid sequences expressed therefrom, which promote the ubiquitylation-mediated, auto-degradation of c-IAP....

20060019337 - Dual expression vector system and screening methods: The present invention features vectors that contain a promoter effective for expression in bacterial cells and a promoter effective for expression in insect cells. The dual promoter system allows use of the same vector in both host cell systems so that construction of only a single vector is needed to...

20060019336 - Factor vii or viia - like molecules: Conjugates of Factor VII (FVII) and Factor VIIa (FVIIA) are provided, as are methods for preparing them. Methods for producing novel polypeptides contributing to the production of such conjugates are provided. Methods of treatment by administering a FVII or FVIIa conjugate are provided....

20060019345 - Human gabab receptor 1 promoters: The present invention relates to nucleic acid molecules constituting GABAB receptor 1 promoters P1a and/or P1b, and to methods for screening for compounds which are modulators of GABAB receptor 1 transcription, said methods comprising the use of nucleic acid molecules constituting GABAB receptor P1a and/or P1b promoters....

20060019346 - Identification of a novel bitter taste receptor t2r76 that specifically responds to brucine and prop bitter ligands: Isolated nucleic acids encoding T2R76 polypeptides, recombinantly expressed T2R76 polypeptides, heterologous expression systems for recombinant expression of T2R76 polypeptides, assay methods employing the same, and methods for altering taste perception via administration of a T2R76 modulator. These T22R76 polypeptides can be expressed alone or co-expressed with another T2R polypeptide, preferably...

20060019342 - Increasing the production of recombinant antibodies in mammalian cells by site-directed mutagenesis: The present invention relates to a reliable, reproducible method for improving the producibility of an antibody. More specifically, this invention provides a method for modifying the heavy chain of an antibody to improve its producibility in eukaryotic cells. Additionally, the method of the invention may improve both antibody producibility and...

20060019339 - Measurement of bioactive hepcidins: The present invention concerns a method for the oxidative refolding of a hepcidin polypeptide to a form that is mature, bioactive and folded as in the native configuration and molecular mass; a method for measuring the level of native, bioactive hepcidin in a vertebrate animal; a method for measuring the...

20060019348 - Method of mass producing silk protein and gene recombinant silk-like protein with added functionality: A method of producing silk or silk-like protein wherein silk or a silk-like polymer comprising at least one protein selected from among domestic silkworm fibroin, wild silkworm fibroin, elastin and fibronectin, and essentially comprising the aforesaid domesticated silkworm fibroin or silkworm fibroin is designed. The minimum unit of the silk...

20060019343 - Pichia methanolica secretory signal: Novel Pichia methanolica secretory signal polypeptides, polynucleotides encoding the polypeptides, and related compositions and methods of using are disclosed. Methods of producing large amounts of recombinant proteins by employing DNA constructs having a polypeptide of interest preceded by a novel Pichia methanolica secretory signal sequence....

20060019349 - Position dependent recognition of gnn nucleotide triplets by zinc fingers: The specificity of binding of a zinc finger to a triplet or quadruplet nucleotide target subsite depends upon the location of the zinc finger in a multifinger protein and, hence, upon the location of its target subsite within a larger target sequence. The present disclosure provides zinc finger amino acid...

20060019338 - Primate prokineticin and prokineticin receptor polypeptides, related compositions and methods: The invention provides an isolated squirrel monkey prokineticin receptor 2 (PKR2) polypeptide containing the amino acid sequence referenced as SEQ ID NO:2; an isolated chimpanzee PKR2 containing the amino acid sequence referenced as SEQ ID NO:4; and an isolated rhesus monkey prokineticin receptor 1 (PKR1) referenced as SEQ ID NO:30....

20060019350 - Recombinant negative strand rna virus expression system and vacccines: Recombinant negative-strand viral RNA templates are described which may be used with purified RNA-directed RNA polymerase complex to express heterologous gene products in appropriate host cells and/or to rescue the heterologous gene in virus particles. The RNA templates are prepared by transcription of appropriate DNA sequences with a DNA-directed RNA...

20060019351 - Enhanced secretion of a polypeptide by a microorganism: Described herein are methods for the enhanced production of secreted proteins. The secretion of a protein of interest having a substantially non-polar carboxy tail is enhanced by the placement of charged amino acid residues at the carboxy terminus either by adding to the native peptide or by replacing, i.e., substituting,...

20060019352 - Methods of increasing the production of cobalamins using cob gene expression: Novel polypeptides involved in the biosynthesis of cobalamines and/or cobamides, in particular coenzyme B12, genetic material responsible for expressing these polypeptides, and a method for preparing them, are described. A method for amplifying the production of cobalamines, and particularly coenzyme B12, using recombinant DNA techniques, are also described....

20060019353 - Protein-nucleic acid conjugate for producing specific nucleic acid: This invention provides inter alia an in vitro process for producing multiple specific nucleic acid copies in which the copies are produced under isostatic conditions, e.g., temperature, buffer and ionic strength, and independently of any requirement for introducing an intermediate structure for producing the copies. In other aspects, the invention...

20060019354 - Compositions and methods of using a regulator of biopolymer production: The present invention provides nucleic acid sequences and variants thereof capable of modulating exopolysaccharide production in Sphingomonas, and provides methods of using such nucleic acid sequences to generate bacteria that hyper-produce exopolysaccharide in slime form....

20060019355 - L-amino acid-producing microorganism and method for producing l-amino acid: L-amino acids are produced by culturing a microorganism which has an ability to produce the L-amino acid, but has been modified so that expression of the ybjE gene has been enhanced. The L-amino acid is collected from the culture medium or from the microorganism....

20060019356 - Polynucleotides encoding polypeptides involved in intermediates metabolism of the central metabolic pathway in methylophilus methylotrophus: The present invention provides polypeptides and polynucleotides involved in central intermediates metabolism in Methylophilus methylotrophus and methods of producing amino acids in microorganisms having enhanced or attenuated expression of these polypeptides and/or polynucleotides....

20060019357 - Nucleotide sequences which code for the rpsl gene: An isolated polynucleotide comprising a polynucleotide sequence chosen from the group consisting of a) polynucleotide which is identical to the extent of at least 70% to a polynucleotide which codes for a polypeptide which comprises the amino acid sequence of SEQ ID No. 2, b) polynucleotide which codes for a...

20060019358 - Process for the preparation of fexofenadine:

20060019359 - Method for producing optically active 1,2-diols by microorganism cuturing: wherein R is a methyl, ethyl, propyl, chloromethyl, or hydroxyethyl group, in a culture medium whose Ca2+ concentration at the beginning of culturing is controlled and which contains a racemic mixture of the compound as a carbon source; and a second step of recovering the optically active compound remaining in...

20060019360 - Process for producing ethanol: A process for producing ethanol including a combination of biochemical and synthetic conversions results in high yield ethanol production with concurrent production of high value coproducts. An acetic acid intermediate is produced from carbohydrates, such as corn, using enzymatic milling and fermentation steps, followed by conversion of the acetic acid...

20060019361 - Immobilization of cells in a matrix formed by biocompatible charged polymers under laminar flow conditions: This invention relates to a method for immobilizing cells under laminar flow conditions in a matrix formed by biocompatible charged polymers, to a flow device for the use with the method of the present invention, and to uses of the polymer matrices containing the cells immobilized using the method of...

20060019362 - Encapsulation of cells in biologic compatible scaffolds by coacervation of charged polymers: This invention relates to a method for the encapsulation of cells in biologic compatible three dimensional scaffolds and the use of such cells encapsulated in a scaffold. The cells are embedded in a charged polymer that is complex coacervating with an oppositely charged polymer within biologic compatible scaffolds. The polymer...

20060019364 - Acetyl coa carboxylase 2 sequences and methods: The present invention relates generally to novel nucleotide and amino acid sequences, and more particularly to novel human acetyl CoA carboxylase 2 (ACC2) and rat ACC2 sequences. The sequences provided herein can be expressed in a recombinant format. Methods of isolating the ACC2 sequence are also provided, which can be...

20060019363 - Cytosolic phospholipase a2-beta enzymes: The invention provides a novel calcium-independent cytosolic phospholipase A2-Beta enzyme, polynucleotides encoding such enzyme and methods for screening unknown compounds for anti-inflammatory activity mediated by the arachidonic acid cascade....

20060019365 - Ikk-alpha proteins, nucleic acids and methods: The invention provides methods and compositions relating to an IκB kinase, IKK-α, and related nucleic acids. The polypeptides may be produced recombinantly from transformed host cells from the disclosed IKK-α encoding nucleic acids or purified from human cells. The invention provides isolated IKK-α hybridization probes and primers capable of specifically...

20060019366 - Reversibly modified thermostable enzyme compositions and methods of making and using the same: The present invention provides reversibly modified thermostable enzyme compositions and methods for making the same. The present invention also provides methods of using the reversibly modified thermostable enzyme compositions, as well as kits and systems comprising the reversibly modified thermostable enzymes....

20060019367 - Method of producing microbial transglutaminase: The present invention provides a neutral metalloprotease from actinomycetes which selectively cleaves a pro-structure part of a microbial protransglutaminase and a gene encoding said neutral metalloprotease. An active microbial transglutaminase having the pro-structure part cleaved can be obtained by culturing a microorganism into which a gene encoding the neutral metalloprotease...

20060019368 - Constructs for homogenously processed preparations of beta site app-cleaving enzyme: The present invention is directed to engineered polypeptides having BACE activity. In certain embodiments, the polypeptides also comprise an engineered cleavage site. Also provided are polypeptides comprising a prodomain, an engineered cleavage site, and a protease domain; the polypeptides are properly folded and are cleaved at the engineered cleavage site...

20060019369 - Recombinant vector containing infectious human cytomegalovirus genome with preserved wild-type characteristics of clinical isolates: A recombinant vector containing infectious genome of human cytomegalovirus (HCMV) and being useful for the production of reconstituted HCMV virus retaining phenotypic characteristics of a clinical virus isolate including the ability to grow on endothelial cells and to induce microfusion is characterized in that it is obtainable by inserting DNA...

20060019370 - Phage-resistant microorganisms and genetic determinants of phage resistance: There are described new bacterial strains, plasmids derived thereof, a gene sequence included in the plasmids encoding a phage resistance system protein, and a method to confer phage resistance to microorganism cultures....

20060019371 - Macrolide analogs: Combinatorial libraries of polyketides can be obtained by suitable manipulation of a host modular polyketide synthase gene cluster such as that which encodes the PKS for erythromycin. The combinatorial library is useful as a source of pharmaceutically active compounds. In addition, novel polyketides and antibiotics are prepared using this method....

20060019373 - Biosensor with rf signal transmission: A device (1) and method for measuring and or detecting the presence of biomolecules. The device comprises a resonance circuit arranged to operate and emit a resonance frequency (f). The resonance circuit comprises or is coupled to a sensor element (5) for detecting the binding of biomolecules (6a) to binding...

20060019372 - Laser system: A laser system and interferometer are disclosed comprising a laser source for generating a laser beam, and first and second adjustable elements wherein the first and second adjustable elements have limited rotational motion so rotation of the first adjustable element causes deviation of a laser beam in one plane and...

20060019374 - Optical biosensor: An optical biosensor has a total reflection plate so as to totally internally reflect and transmit an incident light, a first grating and a second grating disposed separately on the total reflection plate, and a sensing membrane that is containing an enzyme and a chromogenic reagent and is sandwiched by...

20060019375 - Device for culturing cells, particularly human or animal cells: The invention relates to a device (30) for culturing cells of the most diverse type, particularly human or animal cells. According to the invention, a culture is prepared from cells of at least one specified type in a defined environment, and the cells of the relevant culture are supplied with...

20060019376 - Fermentation chamber and mixing apparatus: The claimed invention provides a fermentation chamber that is inexpensive to produce and use and is capable of providing sufficient agitation of the liquid medium to ensure proper mixing and/or aeration without damaging delicate components. The invention further provides a mixing apparatus for agitating one or more such fermentation chambers....

20060019377 - Flask: The present invention provides a flask having a top piece 20 and a bottom piece 22 with optical properties on the interior surface 24 of which cells are grown. The top piece 20 and the bottom piece 22 are joined by a removable elastomeric seal 26. By disengaging the elastomeric...

20060019378 - Cell culture device and manufacturing method thereof: A low-cost cell culture device with low cytotoxicity and improved cell adhesion is described, manufactured by a simplified coating process. At least the portion for cell culture on the surface of the cell culture device is coated with at least one of ε-poly-L-lysine, further polymerized derivatives of ε-poly-L-lysine and their...

20060019379 - Apparatus and method for cell disruption: An apparatus for disrupting cells or viruses comprises a container having a chamber for holding the cells or viruses. The container includes at least one flexible wall defining the chamber. The apparatus also includes a transducer for impacting an external surface of the flexible wall to generate pressure waves in...

20060019380 - Genetic vaccines directed against bacterial exotoxins: The invention provides a gene transfer vector comprising a humanized nucleic acid sequence encoding an immunogenic portion of one or more exotoxins of Bacillus anthracis and a heterologous sorting signal. The invention also provides a method of producing an immune response against Bacillus anthracis in a host comprising administering to...

20060019381 - Hyperthermic inducible expression vectors for gene therapy and methods of use thereof: Methods and compositions are provided for transgene expression in target cells. Expression constructs using an inducible amplification system to drive expression of a therapeutic gene or other gene of interest in mammalian host cells are provided, as well as methods therefor. Inducible expression of the transgenes at high levels under...

20060019382 - Transcriptionally-activated aav inverted terminal repeats (itrs) for use with recombinant aav vectors: This invention provides transcriptionally-activated AAV ITRs (inverted terminal repeats) which are small and transcriptionally active and uses thereof to optimize the expression of relatively large transgenes packaged in recombinant AAV vectors....

20060019383 - Chemical inhibitors of mismatch repair: Dominant negative alleles of human mismatch repair genes can be used to generate hypermutable cells and organisms. By introducing these genes into cells and transgenic animals, new cell lines and animal varieties with novel and useful properties can be prepared more efficiently than by relying on the natural rate of...

20060019385 - Apparatus and methods for producing and using high-density cells and products therefrom: Disclosed and claimed is apparatus and methods for the growth of cells to high density, products therefrom and uses thereof. Also disclosed and claimed is the use of this method for the growth to high-density insect cells, such as the Spodoptera frugiperda Sf900+ cell line (ATCC: CRL 12579). Further disclosed...

20060019384 - Clonal cell lines derived from bti-tn-5b1-4: Improved insect cell lines, two of which are designated H5CL-B and H5CL-F (both of which are derived from the parental cell line BTI-TN-5B1-4, ATCC CRL 10859), possess the properties of increased production of baculovirus particles, increased expression of foreign proteins using a baculovirus expression system, and increased resistance to cell...

20060019386 - Protein domain related to deafness, osteoarthritis and abnormal cell proliferation: The present invention relates to genetic diagnosis and therapy of diseases of the nervous system (NS). More particularly, it relates to methods to induce neural precursor cells (NPCs) and to the identification of a domain that determines the functionality of polypeptides belonging to the atonal family and its use in...

20060019387 - Liver stem cell: The invention provides a primary liver stem cell and a cell doublet consisting of a hepatocyte and the stem cell, both of which are derived from normal liver tissue. Methods of isolating the cells, genetically altering the cells, and using the cells for transplantation are also within the invention....

20060019388 - Bioreactor for growing cell or tissue cultures: The invention relates to a bioreactor comprising a chamber for containing cells or tissue cultures within a culture medium. The bioreactor also comprises a detector capable of detecting a change in one or more metabolites associated with growth of the cell or tissue cultures within the chamber and a chamber...

20060019389 - Porous plasma protein matrices and methods for preparation thereof: The present invention relates to porous freeze-dried plasma protein matrices having an open channel structure and a concentration gradient of plasma proteins crosslinked by the action of thrombin, and methods of producing said matrices. The compositions of the present invention are useful clinically, per se or as cell-bearing implants....

20060019390 - Patterning substrate and cell culture substrate: The present invention intends primarily to provide such as a cell culture patterning substrate that is used to adhere cells in a highly precise pattern on a base material to culture and a cell culture substrate on which cells is adhered in a highly precise pattern. To attain the above-mentioned...

20060019391 - Liquid-gas-phase exposure reactor for cell culturing: Initiation of growth and cultivation of cells can be performed by introducing the cells into culture compartments of a liquid-gas-phase exposure bioreactor containing a supply chamber in which there are disposed hollow-filament membranes having an inside diameter of no larger than 5 mm, wherein an inner volume of said hollow-filament...

20060019392 - Novel bone mineralization proteins, dna, vectors, expression systems: The present invention is directed to isolated nucleic acid molecules that encode LIM mineralization protein, or LMP. The invention further provides vectors comprising nucleotide sequences that encode LMP, as well as host cells comprising those vectors. Moreover, the present invention relates to methods of inducing bone formation by transfecting osteogenic...

20060019394 - Bovine immunodeficiency virus (biv) based vectors: This invention pertains to BIV constructs encompassing BIV combination vectors, BIV vectors and BIV packaging vectors and particularly the invention pertains to a three vector system comprising: a) a BIV vector construct including a DNA segment from a BIV genome, a packaging sequence to package RNA into virions; a promoter...

20060019395 - Lentiviral vectors and uses thereof: The invention relates to compositions containing polynucleotide vectors capable of expressing a nucleic acid encoding a fusion polypeptide on the surface of a viral particle and/or a eukaryotic cell....

20060019393 - Minimal lentiviral vector system: A lentiviral vector system is described. The system comprises a lentiviral transfer vector and a packaging construct. The transfer vector comprises (a) a 5′ LTR; (b) a 3′ LTR comprising a polyadenylation signal; (c) a minimal packaging signal, (d) (i) at least one heterologous upstream enhancer (UE) sequences, and/or (ii)...

20060019396 - Retroviral vectors with enhanced efficiency of transgene expression and safety: The present application discloses a MoMSV/MoMLV hybrid-based retroviral vector, wherein gag and pol genes are completely removed....

20060019397 - Self-improving classification system: A self-improving classification system classifies specimens based on class identifiers. The system stores specimen profiles in a database that is updated with additional specimen profiles and with follow-up data that corrects classification of specimens that were initially incorrectly classified. Algorithms use the updated database to discover new class identifiers, modify...

20060014134 - Low-temperature storage of suspension samples in suspended sample chambers: A cryostorage device (100) is described that includes at least one data storage device (200) and at least one sample receptacle device (300) with at least one sample chamber (301, 302, etc.) for the uptake of a suspension sample, the at least one sample chamber (301, 302, etc.) being connected...

20060014135 - Assay for distinguishing live and dead cells: Image analysis methods and apparatus are used for distinguishing live and dead cells. The methods may involve segmenting an image to identify the region(s) occupied by one or more cells and determining the presence of a particular live-dead indicator feature within the region(s). In certain embodiments, the indicator feature is...

20060014136 - Method of screening for protection from microbial infection: Screening assays that allow for the identification of agents that modulate host cell response to microbial infection. The methods of the invention provide the ability to measure the reduction in toxicity to the host cell, which is directly related to the ability of a compound to alter the cell's responses...

20060014137 - System for cell-based screening: The present invention provides systems, methods, screens, reagents and kits for optical system analysis of cells to rapidly determine the distribution, environment, or activity of fluorescently labeled reporter molecules in cells for the purpose of screening large numbers of compounds for those that specifically affect neurite outgrowth....

20060014142 - Compositions and methods for detection of hepatitis a virus nucleic acid: Nucleic acid oligomeric sequences and in vitro nucleic acid amplification and detection methods for detecting the presence of HAV RNA sequences in samples are disclosed. Kits comprising nucleic acid oligomers for amplifying and detecting HAV nucleic acid sequences are disclosed....

20060014139 - Five-helix protein: Five-Helix protein, which comprises the three N-helices and at least two, but not three, of the three C-helices of the trimer-of-hairpin structure of HIV gp41, separated by linkers, such as amino acid residue linkers, is disclosed. Six-Helix protein, which includes the three N-helices and the three C-helices of the trimer-of-hairpin...

20060014141 - Method of detecting and quantifying cytomegalovirus: Combinations of oligonucleotide primers which allow specific amplification of the mRNA of the CMV β2.7 gene, RNA amplification using these combinations of oligonucleotide primers and a detection method which uses a fluorescent intercalative dye-labeled oligonucleotide probe to monitor the RNA amplification....

20060014140 - Molecular methods and compositions for detecting and quantifying respiratory viruses: The present invention relates to new methods and nucleic acid sequences for the detection and quantification of human metapneumovirus....

20060014138 - Phage microarray profiling of the humoral response to disease: The present invention relates to compositions and methods for cancer diagnostics, including but not limited to, cancer markers. In particular, the present invention provides methods and compositions for phage microarray profiling of cancer (e.g., prostate, lung, or breast cancer). The present invention further provides novel markers useful for the diagnosis,...

20060014143 - Preparation of potent macrophage activating factors derived from cloned vitamin d binding protein and its domain and their therapeutic usage for cancer, hiv-infection and osteopetrosis: Vitamin D-binding protein (Gc protein) and its small domain (approximately ⅕ of the Gc peptide also known as domain III) were cloned via a baculovirus vector. The cloned Gc protein and the cloned domain (Cd) peptide were treated with immobilized β-galactosidase and sialidase to yield macrophage activating factors, GcMAFc and...

20060014167 - Amplification of trace amounts of nucleic acids: Methods of reducing background during amplification of small amounts of nucleic acids employ careful analysis of sources of low level contamination. Ultraviolet light can be used to reduce nucleic acid contaminants in reagents and equipment. “Primer-dimer” background can be reduced by judicious design of primers. We have shown clean signal-to-noise...

20060014191 - Analog probe complexes: The present invention relates to the detection of target sequences. The present description discloses compositions and methods involving analog nucleic acids, such as PNA and L-DNA, for the detection of nucleic acids. Additionally, hybrid detectable markers are provided....

20060014200 - Apparatus for analysis of a nucleic acid amplification reaction: An apparatus for determining a threshold value (e.g., a threshold cycle number or a time value) in a nucleic acid amplification reaction comprises a detection mechanism for measuring, at a plurality of different times during the amplification reaction, at least one signal whose intensity is related to the quantity of...

20060014172 - Aptamer-nanoparticle conjugates and method of use for target analyte detection: The present invention provides aptamer probes, nanoparticle-aptamer conjugate probes, aptamer arrays, methods of detecting target analytes in a sample comprising detecting binding of a target analyte with aptamer probes, method of detection, and kits....

20060014187 - Association of single nucleotide polymorphisms in ppargamma with osteoporosis: The current invention is based on the discovery that the Prol2Ala and VN102 single nucleotide polymorphisms in the PPARγ2 provides a method of determining a susceptibility to osteoporosis by detecting the presence of PPARγ of the alleles....

20060014161 - Combination comprising biochip and optical detection device: The invention relates to a method and apparatus for the routine analysis of samples that contain biomolecules. The invention uses biochips or arrays in the format of conventional 35 mm film slides. These film slide arrays allow employing commercially available detection, storage and processing equipment. The use of array technology...

20060014164 - Computer software to assist in identifying snps with microarrays: The present invention is a method to assist in the identification of single nucleotide polymorphisms (SNP) from microarray hybridization data. Data from hybridization protocols run on microarrays often have variations in the data resulting from variations in hybridization conditions and efficiencies and variations in optical intensities. An algorithm is described...

20060014189 - Controls for determining reaction performance in polynucleotide sequence detection assays: The present teachings relate to methods, compositions, and kits for detecting one or more target polynucleotide sequences in a sample. In some embodiments of the present teachings, probes are hybridized to complementary target polynucleotides and are ligated together to form a ligation product. Some embodiments of the present teachings comprise...

20060014194 - Cyclic peptides for treatment of cachexia: e

20060014181 - Detection of dna mismatches and oxidative lesions: The present invention describes methods for directly labeling the 3′-phosphate end at a nucleotide site. Further, as internal 3′-phosphate termini on DNA duplexes are also associated generally with oxidative lesions, these methods provide a general strategy for labeling, and therefore, detecting the frequency of oxidative DNA lesions. The present invention...

20060014150 - Development of therapeutics for the treatment of endotoxin-meidated diseases: The subject invention comprises a method for identifying an evolutionarily meaningful nucleotide change in a primate's TLR4 polynucleotide. It further comprises methods for identifying agents that interact with the corresponding evolutionarily meaningful amino acid change so as to modulate the function of the TLR4 polypeptide, thereby attenuating activation of the...

20060014147 - Diagnostics and therapeutics for diseases associated with oxytocin receptor (oxtr): The invention provides a human OXTR which is associated with the hematological diseases, cardiovascular diseases, disorders of the peripheral and central nervous system, cancer diseases, respiratory diseases, metabolic diseases. The invention also provides assays for the identification of compounds useful in the treatment or prevention of hematological diseases, cardiovascular diseases,...

20060014163 - Dna-binding polymers: Methods and compositions are provided for forming complexes between dsDNA and novel DNA-binding polymers comprising N-terminal thiophene-containing moieties which exhibit selectivity for T-A base pairs. By appropriate choice of target sequences and DNA-binding polymers, complexes comprising polymer-DNA are obtained with high association constants. The formation of complexes can be used...

20060014202 - Electrical connection structure, production method thereof, and electric wiring method: An electrical connection structure that is able to electrically connect wiring to a biopolymer, a production method of the electrical connection structure, and an electric wiring method which is able to perform wiring on a nanometer-scale. A first aspect of the production method of the present invention uses a carbon...

20060014203 - Electrical connection structure, production method thereof, and electric wiring method: An electrical connection structure that is able to electrically connect wiring to a biopolymer, a production method of the electrical connection structure, and an electric wiring method which is able to perform wiring on a nanometer-scale. A first aspect of the production method of the present invention uses a carbon...

20060014183 - Extendable probes: The invention relates to probes which are extendable useful as PCR probes and in probe libraries. The invention further relates to prevention of replication of a primer extension product in PCR reactions....

20060014175 - Functionalized fluorescent nanocrystal detection system: The present invention include fluorescent nanocrystals which have high fluorescence intensity, are water soluble, exhibit physical and chemical stability, and whose spectral properties are detectably modified as the size of functional groups bonded to the nanocrystal surface change when contacted with target molecules in a sample. The molecules in the...

20060014180 - Human phosphatase ret31, and variants thereof: The present invention provides novel polynucleotides encoding human phosphatase polypeptides, fragments and homologues thereof. Also provided are vectors, host cells, antibodies, and recombinant and synthetic methods for producing said polypeptides. The invention further relates to diagnostic and therapeutic methods for applying these novel human phosphatase polypeptides to the diagnosis, treatment,...

20060014162 - Identification and characterization of racemases, definition of protein signatures, and a test for detecting d-amino acid and for screening molecules capable of inhibiting the activity of racemase, especially proline racemase: This invention provides identification and characterization of racemases and definition of protein signatures of these racemases. This invention also provides identification of nucleic acid molecules encoding a peptide consisting of a motif characteristic of the protein signatures, and to the peptides consisting of these motifs. Antibodies specific for the peptides...

20060014148 - Igg fc/hiv-gp120/c3d fusion protien: The present invention relates, in general, to an immunogen and, in particular, to an immunogen for inducing antibodies that neutralize a wide spectrum of HIV primary isolates. The invention also relates to a method of inducing anti-HIV antibodies using same....

20060014197 - In vivo screening methods for identifying inhibitors of rna polymerases: In vivo screening methods for identifying inhibitors of RNA polymerase (RNAP) are provided by the present invention. In certain embodiments, methods according to the invention include steps of: (a) expressing a test RNAP in a cell containing a first reporter; (b) expressing a control RNAP in the cell of step...

20060014179 - Inferring function from shotgun sequencing data: Methods are described for detecting genes that encode toxic proteins using maps derived from shotgun libraries by determining the presence of gaps in clone start sites on either side of open reading frames. The method is exemplified by identifying a previously unknown restriction endonuclease gene....

20060014185 - Integrated nucleic acid analysis: The present invention relates to an integrated method of nucleic acid analysis, and more particularly to a simplified sample pre-treatment, which renders the method more easily automated, where the sample is provided on or applied onto a solid matrix and the subsequent amplification and detection steps are performed in one...

20060014195 - Investigation of dna samples: A method of investigating a DNA sample is provided involving taking at least two sub-samples from the sample, amplifying the sub-samples, analysing the sub-samples to obtain identity information and amount of that identity about one or more alleles indicated as present in the sub-samples in respect of 5 or more...

20060014198 - Method for apparatus for detecting luminescence light from a porous support structure: Method and apparatus for detecting chemical and/or biochemical reactions and/or bindings. A two-dimensional support structure which has, distributed over at least one surface region, a multiplicity of pores which extend continuously from one surface of the support structure to the opposite surface, is provided wherein the pores are bounded by...

20060014174 - Method for the detection and identification of circulating micrometastatic cancer cells: The invention relates to an method for the detection of cancer cells of epithelium origin expressing the Ep-Cam antigen and identification of circulating micrometastatic cancer cells in peripheral blood tissues and other body fluids of patients suffering from cancer of epithelial origin, using a sample of total blood, body fluids...

20060014146 - Method of creating a library of bacterial clones with varying levels of gene expression: The present invention relates to a method of creating DNA libraries that include an artificial promoter library and/or a modified ribosome binding site library and transforming bacterial host cells with the library to obtain a population of bacterial clones having a range of expression levels for a chromosomal gene of...

20060014168 - Method of diagnosis of inclusion body myopathy-paget bone disease-frontotemporal dementia syndrome: The present invention is based on the discovery of a genetic basis for inclusion body myopathy-Paget bone disease-frontotemporal dementia syndrome (IBMPFD). We have determined that genetic alterations in the gene encoding vasolin containing protein (VCP) is responsible for IBMPDF syndrome. In particular, we have identified six missense mutations within VCP...

20060014145 - Methods and articles for high throughput analysis of biomolecular interactions: Methods, compositions articles of manufacture, and kits for characterizing biomolecular interactions are provided. The interactions may involve various combinations of proteins, lipids, carbohydrates and small molecules. For example, interactions between polynucleotide segments and polynucleotide binding protein(s) may be characterized....

20060014182 - Methods and compositions for amplification of rna sequences: The invention provides methods for linear and exponential amplification of RNA. They are particularly suitable for amplifying a plurality of RNA species in a sample. The methods are based on hybridization of polynucleotide comprising a propromoter sequence to a primer extension product to generate an intermediate polynucleotide capable of driving...

20060014152 - Methods and reaction mixture reagent for increasing the specificity and fidelity of polymerase reactions: A method for reducing the efficiency of primer extension by polymerase enzymes when the 3′ end of a primer or growing nucleic acid chain does not hybridize perfectly with the target, for increasing the selectivity of single nucleotide mutation or gene analyses, for suppressing false positive results and for enhancing...

20060014190 - Methods for analyzing short tandem repeats and single nucleotide polymorphisms: Methods for genotyping a sample comprising nucleic acid are provided. The methods comprise analyzing STR and SNP loci....

20060014186 - Methods for genotype screening of a strain disposed on an adsorbent carrier: The present invention provides a method to rapidly provide genoty