Chemistry: molecular biology and microbiology inventions 01/06

20060019233 - Delivery of high cell mass in a syringe and related methods of cryopreserving cells: This invention relates to methods and apparatus for cryopreserving biological materials for extended periods of time. In an exemplary embodiment, the method comprises suspending biological materials in a cryosolution, freezing the biological materials in the apparatus, and removing the frozen biological materials from the apparatus to thaw them for use....

20060019234 - Modern blood banking employing improved cell preservation composition: An improved anticoagulant is based on a higher level of citric acid than is usual (at least about 0.2% weight by volume). The higher citrate is combined with an amino acid as a counterion. The amino acid prevents cellular damage often caused by elevated citrate levels. The amino acid citrate...

20060019237 - In vitro wound healing assay and device: Devices for creating model wounds in vitro are provided, along with kits and systems including the devices. Methods for making such devices and for modeling wounds are also provided....

20060019236 - Membrane fusion assay: The present invention relates to an assay for detecting membrane fusion. More particularly the present invention relates to assays for detecting exocytosis and/or endocytosis wherein membrane fusion is detected by an increase in fluorescence of a lipophilic fluorescent dye due to fluorescence resonance energy transfer (FRET) occurring from one lipophilic...

20060019235 - Molecular and functional profiling using a cellular microarray: Cells are profiled with respect to their expression of cell surface molecules, and ability to respond to external stimulus in the microenvironment. External stimuli include cell-cell interactions, response to factors, and the like. The cells are arrayed on a planar or three-dimensional substrate through binding to immobilized or partially diffused...

20060019242 - Detection of herpes simplex virus: The invention provides methods to detect herpes simplex virus (HSV) in biological samples and further to distinguish between HSV-1 and HSV-2. Primers and probes for the differential detection of HSV-1 and HSV-2 are provided by the invention. Articles of manufacture containing such primers and probes for detecting HSV are further...

20060019245 - Hcv variants: HCV variants are described. The variants include polynucleotides comprising non-naturally occurring HCV sequences and HCV variants that have a transfection efficiency and ability to survive subpassage greater than HCV that have wild-type polyprotein coding regions. Expression vectors comprising the above polynucleotides and HCV variants are also described, as are the...

20060019243 - Method and device for mixing samples on a support: A method for detecting analytes in a liquid is provided in which the liquid is subjected to a mixing treatment on an area of a support which has in particular immobilized reactants, wherein in the mixing treatment the liquid is impinged upon by a stream of gas that sweeps across...

20060019238 - Method for identifying protein-protein interactions: The properties of yeast help, a type I ER membrane protein which is involved in the unfolded protein response (UPR), have been exploited to develop â. system for the detection and study of interactions between extracellular and/or membrane proteins. In the system, proteins of interest are fused to the lumenal...

20060019239 - Method of preventing infections from bioterrorism agents with immunostimulatory cpg oligonucleotides: The present disclosure relates to a method of preventing or treating an infection caused by a bioterrorism agent, specifically to a method of increasing an immune response to a bioterrorism agent using an oligodeoxynucleotide including a CpG motif, and a method of enhancing the immunogenicity of a vaccine against a...

20060019241 - Na+ and ci-coupled transport system for endogenous opioid peptides: The present invention provides the identification and characterization of an endogenous opioid peptide transporter, and uses thereof....

20060019244 - Planar optical waveguide based sandwich assay sensors and processes for the detection of biological targets including protein markers, pathogens and cellular debris: An assay element is described including recognition ligands bound to a film on a single mode planar optical waveguide, the film from the group of a membrane, a polymerized bilayer membrane, and a self-assembled monolayer containing polyethylene glycol or polypropylene glycol groups therein and an assay process for detecting the...

20060019240 - Posh nucleic acids, polypeptides and related methods: The application discloses novel polypeptides and nucleic acids involved in a variety of biological processes, including viral reproduction. Related methods and compositions are also described....

20060019246 - Synthetic c-glycolipid and its use for treating cancer, infectious diseases, and autoimmune diseases: wherein X is O or NH; R′ is a hydrocarbon chain; R3 and R4 are hydrogen, OH or a monosaccharide; R5 is hydrogen or a monosaccharide; Q′ is optionally present and may be a C1-10 hydrocarbon; X′ is optionally present and may be O, S or NR8; and Q3 may...

20060019262 - Activation induced deaminase (aid): The present invention identifies that the expression of Activation Induced Deaminase (AID) or its homologues in cells confers a mutator phenotype and thus provides a method for generating diversity in a gene or gene product as well as cell lines capable of generating diversity in defined gene products. The invention...

20060019253 - Amplicon melting analysis with saturation dyes: Methods are provided for nucleic acid analysis wherein a target nucleic acid that is at least partially double stranded is mixed with a dsDNA binding dye having a percent saturation of at least 50% to form a mixture. In one embodiment, the nucleic acid is amplified in the presence of...

20060019285 - Analysis of intracellular modifications: Improved methods of determining the intracellular state of a protein as well as modifications of the protein are provided by introducing a surrogate fusion protein comprising a member of an enzyme fragment complementation complex and a target protein. After exposing cells transformed with the surrogate fusion protein to a change...

20060019299 - Bio-molecules detecting apparatus using electromagnetic induction and detecting method using the same: Provided are a bio-molecules detecting apparatus using electromagnetic induction and a detecting method using the same. An exemplary apparatus includes: a cantilever of which one end is fixed and the other end is set up to be movable; a first metal formed on a plane of the cantilever and receiving...

20060019259 - Characterization of biopolymers by resonance tunneling and fluorescence quenching: The present invention provides a method and apparatus for determining the identity of a monomeric residue of a biopolymer. The apparatus comprises a substrate having a nanopore, a potential-producing element for producing a ramped potential across electrodes adjacent to the nanopore, and a quenchable excitable moiety adjacent to the nanopore....

20060019291 - Cloning system for construction of recombinant expression vectors: Cloning systems are provided for constructing expression vectors. In one aspect of the invention, a kit is provided for constructing one or more recombinant expression vectors. The kit comprises: a linear driver DNA comprising a promoter sequence, a donor recombination site, and at least one selectable marker, the linear driver...

20060019283 - Common marmoset-derived hprt gene and use thereof: The present invention provides a common marmoset-derived hypoxanthine-guanine phosphoribosyltransferase gene and use thereof....

20060019289 - Compositions and methods for gene expression analysis: The present disclosure provides methods and composition to detect or quantitate one or more target sequences....

20060019257 - Compositions and methods for increasing protein production: Compositions and methods for increasing protein production are provided....

20060019256 - Compositions and methods for treating and diagnosing cancer: The present invention relates to compositions and methods for treating, characterizing, and diagnosing cancer. In particular, the present invention provides gene expression profiles associated with solid tumor stem cells, as well as novel stem cell cancer markers useful for the diagnosis, characterization, and treatment of solid tumor stem cells....

20060019273 - Detection card for analyzing a sample for a target nucleic acid molecule, and uses thereof: The present invention relates to a detection card made of a first planar element having a detection chip. The detection chip has two or more electrically separated conductors fabricated on a substrate and capture probes attached to the conductors, such that a gap exists between the capture probes on the...

20060019306 - Detection of ochratoxin a producing fungi: Nucleotide or amino acid sequences that may be used in the detection and/or identification fo an ochratoxigenic fungus or in the construction of an atoxigenic strain of an ochratoxigenis fungus. The fungus may be of the genus Aspergillus, species carbonarius, niger, alliaceus, or foetidux. The fungus may also be of...

20060019305 - Diagnosis and treatment of ptp related disorders: The present invention relates to PTP05 polypeptides and PTP10 polypeptides, nucleic acids encoding such polypeptides, cells, tissues and animals containing such nucleic acids, antibodies to such polypeptides, assays utilizing such polypeptides, and methods relating to all of the foregoing. Methods for treatment, diagnosis, and screening are provided for PTP05 and/or...

20060019272 - Diagnosis of disease and monitoring of therapy using gene expression analysis of peripheral blood cells: Disclosed are methods to diagnose a patient that has a pulmonary disease, and particularly, pulmonary arterial hypertension, using biomarkers that are differentially regulated in the peripheral blood cells of patients with such disease as compared to individuals that do not have the disease. Also disclosed are methods to diagnose a...

20060019249 - Diagnostic conjugate useful for intracellular imaging and for differentiating between tumor-and non-tumor cells: Described is a diagnostic conjugate comprising (a) a transmembrane module (TPU), (b) an address module (AS), preferably an antisense peptide nucleic acid (PNA), and (c) a signalling module (SM). Said conjugate is useful for intracellular imaging, preferably via MRI, and, e.g., for differentiating between tumor- and non-tumor cells....

20060019277 - Digital amplification for detection of mismatch repair deficient tumor cells: The detection of mutations in fecal DNA represents a promising, non-invasive approach for detecting colorectal cancers in average risk populations. One of the first practical applications of this technology involves the examination of microsatellite markers to sporadic cancers with mismatch repair deficiencies. As such cancers nearly always occur in the...

20060019252 - Genes and polypeptides relating to hepatocellular or colorectal carcinoma: The present application provides novel human genes WDRPUH and KRZFPUH and PPIL1 whose expression is markedly elevated in a great majority of HCCs and colorectal cancers, respectively, compared to corresponding non-cancerous tissues, as well as novel human gene APCDD1 whose expression is elevated in primary colon cancers and down-regulated in...

20060019295 - Genomic barcoding for organism identification: The invention disclosed herein relates to the comparison of whole genomes to identify short oligonucleotide sequences that are specific to a single organism. In some embodiments of the invention, combinations of species-specific oligonucleotides are used to produce specific amplification products. In some embodiments, isolate-specific oligonucleotides are used to detect and...

20060019270 - Global dna methylation assessment using bisulfite pcr: The present invention provides a method and assay for determining global methylation using the methylation status of repetitive DNA elements as a surrogate marker. Also provided by the invention is a method for determining efficacy of a DNA methylation inhibiting drug by using changes in repetitive DNA methylation as a...

20060019297 - Glyceraldehyde-3-phosphate dehydrogenase and phosphoglycerate mutase regulatory sequences for gene expression in oleaginous yeast: The regulatory sequences associated with the Yarrowia lipolytica glyceraldehyde-3-phosphate dehydrogenase (gpd) and phosphoglycerate mutase (gpm) genes have been found to be particularly effective for the expression of heterologous genes in oleaginous yeast. The promoter regions of the invention, intron and enhancer have been shown to drive high-level expression of genes...

20060019286 - High throughput methods relating to microrna expression analysis: The invention relates to methods and compositions for microRNA expression analysis using microarrays....

20060019290 - Identification of markers in esophageal cancer, colon cancer, head and neck cancer, and melanoma: Methods for identifying expression of markers indicative of the presence of esophageal, a squamous cell cancer, a squamous cell cancer of the head and neck, colon cancer and melanoma are provided. Also provided are articles of manufacture useful in such methods and compositions containing primers and probes useful in such...

20060019284 - Identification of polynucleotides for predicting activity of compounds that interact with and/or modulate protein tyrosine kinases and/or protein tyrosine kinase pathways in lung cancer cells: The present invention describes polynucleotides that have been discovered to correlate to the relative intrinsic sensitivity or resistance of cells, e.g., lung cell lines, to treatment with compounds that interact with and modulate, e.g., inhibit, protein tyrosine kinases, such as, for example, members of the Src family of tyrosine kinases,...

20060019278 - Marker for prenatal diagnosis and monitoring: The present invention relates to new methods for diagnosing a pregnancy-associated disorder by analyzing fetal DNA present in the mother's blood. More specifically, this invention relies on the discovery that the maspin gene is differentially methylated in fetal DNA and in maternal DNA and provides these new diagnostic methods, which...

20060019302 - Method and apparatus for applying fluids to a biological sample: The invention is directed to a method of contacting a biological sample with a solution, comprising the steps of moving a curved surface wetted with the solution in proximity to the biological sample whereby the distance separating the wetted curved surface and the biological sample is sufficient to form a...

20060019247 - Method and apparatus for nucleic acid sequencing and identification: The methods and apparatus 100 disclosed herein are of use for sequencing and/or identifying nucleic acids 230, 310. Nucleic acids 230, 310 containing labeled nucleotides 235, 245, 315 may be synthesized and passed through nanopores 255, 310. Detectors 257, 345 operably coupled to the nanopores 255, 310 may detect the...

20060019282 - Method and unit for detecting an interaction such as hybridization, bioassay plate provided with a number of such detecting units, system for detecting an interaction such as hybridization, and reagent kit: Disclosed is a method for the detection of an interaction between substances. According to the method, a salt-containing HEPES buffer is allowed to exist in a reaction region that provides a place of interaction for the interaction. The concentration of the salt in the salt-containing HEPES buffer may be adjusted...

20060019300 - Method for in vitro molecular evolution of protein function: The invention provides a method for generating a polynucleotide sequence or population of sequences from parent single stranded polynucleotide sequences encoding one or more protein motifs, comprising the steps of a) providing single stranded DNA constituting plus and minus strands of parent polynucleotide sequences; b) digesting the single stranded polynucleotide...

20060019264 - Method for isolation of independent, parallel chemical micro-reactions using a porous filter: The present invention relates to methods and apparati for conducting densely packed, independent chemical reactions in parallel in a substantially two-dimensional array. Accordingly, this invention also focuses on the use of this array for applications such as DNA sequencing, most preferably pyrosequencing, and DNA amplification....

20060019260 - Method for tapping the immunological repertoire: The present invention relates to a method for isolating from the immunological gene repertoire a gene coding for a receptor having the ability to bind a preselected ligand. Receptors produced by the gene isolated by the method, particularly catalytic receptors, are also contemplated....

20060019250 - Method for the detection of carcinoembryonic agent and cancer diagnosis method based thereon: The present invention provides a method of detecting carcinoembryonic antigen (CEA) comprising detecting a DNA sequence comprising the sequence: GATATAGCAGCCCTGGTGTAGTTTCTTCATTTCAGGAAGACTG. The invention also provides a method of diagnosis of disseminated cancer comprising obtaining a cell sample, extracting DNA from the cell and detecting the presence of the sequence: GATATAGCAGCCCTGGTTSGTTCTTCATTTCAGGAAGACTG....

20060019251 - Method for the qualitative and quantitative determination of genetic material: The invention relates to a method for qualitatively and quantitatively determining genetic material which contains a known target sequence which is characteristic for the material and which can be present alongside, or in a mixture with, other genetic material, in which a suspension which contains the genetic material possessing the...

20060019303 - Method to identify and analyze genes having modified expression in stimulated t cells: A method of identifying genes involved in the stimulation of primary T cells comprising the steps of: a) contacting experimental cells with a stimulating agent; b) preparing RNA from said experimental cells at one or more stimulation phases; c) measuring the level of gene expression in the cells; d) comparing...

20060019263 - Methods and apparatuses for analyzing polynucleotide sequences: Methods for high speed, high throughput analysis of polynucleotide sequences, and apparatuses with which to carry out the methods are provided in the invention....

20060019258 - Methods and compositions for detection of small interfering rna and micro-rna: The invention provides a method of distinguishing small RNA from mRNA by contacting a biological isolate with a phosphate reactive reagent having a label moiety under conditions wherein the label moiety is preferentially added to the 5′ phosphate of small RNA over the 5′ cap structure of mRNA and distinguishing...

20060019276 - Methods and devices for nucleic acid sequence determination: Methods of the invention comprise methods and devices for nucleic acid sequence determination. Generally, the invention relates to preparing a substrate for sequencing a target nucleic acid....

20060019267 - Methods and kits for analyzing polynucleotide sequences: The present invention features methods for analyzing a sequence of a target polynucleotide by detecting incorporation of a nucleotide into its complementary strand, where the polynucleotides may be bound at high density and at single molecule resolution. The invention also features labeling moieties and blocking moieties, which facilitate chain termination...

20060019279 - Methods for target molecule detection using siderophores and related compositions: The invention provides methods for isolating a target molecule from a sample. In an embodiment, the method involves contacting a sample with a capture agent, the agent comprising a siderophore and a transition metal cation, under conditions wherein the agent is capable of binding a target molecule to form a...

20060019296 - Methods of indentifying modulators of the fgf receptor: The present invention provides fragments of SNT and FGFR which can form a binding complex that is amenable to structural determinations by NMR spectroscopy. The three-dimensional structural data is also included as part of the invention. In addition, the present invention provides methodology for related structure based rational drug design...

20060019301 - Methods of producing mutant polynucleotides: The present invention relates to methods of producing mutants of a polynucleotide and to mutant polynucleotides and artificial variants encoded by the mutant polynucleotides....

20060019288 - Methods, reaction mixtures, and kits for ligating polynucleotides: The present teachings pertain to methods, reaction mixtures, and kits for ligating polynucleotides. In some embodiments, a heat-activatable ligation agent, a phosphorylation agent, and a decontamination agent are included in the same ligation reaction mixture with at least one probe set, at least one linker set, and at least one...

20060019268 - Molecular markers of cisplatin resistance in cancer and uses thereof: The present invention relates generally to the fields of molecular biology and medicine. More particularly, it concerns cancer treatments and the identification and use of markers for cancer resistance to a platinum-drug based therapy therapy. In certain embodiments, the present invention provides diagnostic and/or prognostic methods involving a collection of...

20060019248 - Mutant sh-3 binding protein compositions and methods: The invention provides mutant SH3-binding protein (SH3BP2) nucleic acids, polypeptides, and agents which selectively bind to the mutant SH3BP2 molecules and which do not bind to the wild type SH3BP2 molecules. Methods for selecting agents which inhibit mutant SH3BP2 expression, as well as diagnostic and therapeutic methods which utilize the...

20060019280 - Mutations in kit confer imatinib resistance in gastrointestinal stromal tumors: The present invention relates to methods and compositions concerning resistance to a drug for cancer comprising aberrant KIT signal, such as aberrant KIT sequence or expression. In a specific embodiment, the cancer is also initially responsive to imatinib therapy, such as in gastrointestinal stromal tumors (GISTs). In particular embodiments, a...

20060019293 - Mutations of the 5' region of the human 5-ht1a gene, associated proteins of the 5' region and a diagnostic test for major depression and related mental illnesses: Clinical response to antidepressant compounds correlates with a selective down-regulation of presynaptic 5-HT1A receptors in serotonergic raphe neurons. Thus regulation of the 5-HT1A receptor gene could play a crucial role in the treatment or etiology of major depression. The promoter and repressor activities of the human 5-HT1A receptor gene have...

20060019274 - Nano-pcr: methods and devices for nucleic acid amplification and detection: Methods, devices, and compositions are described that provide for amplification of nucleic acid sequences without reliance upon temperature cycling, thus freeing the methods from conventional benchtop thermal cycling devices. Denaturation of double stranded nucleic acids, primer annealing, and precision control over primer extension by polymerase can be accomplished by applying...

20060019266 - Novel nucleotide mixture for improved nucleic acid amplification performance: The present invention relates to modification of amplification buffer used in amplification reactions. The modifications result in a significant improvement in results of amplification In particular, the present invention provides methods and buffers for performing an amplification reaction utilizing a buffer comprising nucleotide triphosphates comprising treating the buffer to substitute...

20060019275 - Nucleic acids encoding proteins involved in sensory transduction: The invention provides isolated nucleic acid and amino acid sequences of sensory cell specific polypeptides, antibodies to such polypeptides, methods of detecting such nucleic acids and polypeptides, and methods of screening for modulators of sensory cell specific polypeptides....

20060019255 - Nucleotide sequences specific to brucella and methods for the detection of brucella: Described herein are the identification of nucleotide sequences specific to Brucella that serves as a marker or signature for identification of this bacterium. In addition, forward and reverse primers and hybridization probes derived from these nucleotide sequences that are used in nucleotide detection methods to detect the presence of the...

20060019254 - Nucleotide sequences specific to yersinia pestis and methods for the detection of yersinia pestis: Described herein are the identification of nucleotide sequences specific to Yersinia pestis that serves as a marker or signature for identification of this bacterium. In addition, forward and reverse primers and hybridization probes derived from these nucleotide sequences that are used in nucleotide detection methods to detect the presence of...

20060019261 - Rna-binding protein: The present invention provides Synaptotagmin-binding and yet RNA-binding proteins and genes encoding the proteins. The invention relates to a recombinant protein selected from the group consisting of the following (a) and (b), as well as a gene encoding the protein: (a) a protein comprising the amino acid sequence as shown...

20060019287 - Secreted and transmembrane polypeptides and nucleic acids encoding the same: The present invention is directed to novel polypeptides and to nucleic acid molecules encoding those polypeptides. Also provided herein are vectors and host cells comprising those nucleic acid sequences, chimeric polypeptide molecules comprising the polypeptides of the present invention fused to heterologous polypeptide sequences, antibodies which bind to the polypeptides...

20060019292 - Sequence specific recombinase-based methods for producing intron containing vectors and compositions for use in practicing the same: Methods are provided for producing a vector that includes at least one splicable intron. In the subject methods, intron containing vectors are produced from donor and acceptor vectors that each include a site specific recombinase site, where the subject donor and acceptor vectors further include splice donor and acceptor sites...

20060019298 - Sequencing of modified nucleic acid molecules: Modified nucleic acids are used for many purposes in research, diagnostic, and treatment protocols. However, direct sequencing of such molecules is generally considered to require special conditions. Methods of making a cDNA using a modified nucleic acid molecule and sequencing methods of sequencing a modified nucleic acid molecule are described....

20060019304 - Simultaneous analysis of multiple genomes: The invention provides methods for multiplexing readouts from multiple hybridization-based assays that each comprise one or more hybridization or annealing steps and one or more enzymatic processing steps. In one aspect, the invention permits simultaneously analysis of a plurality of genomes by separately hybridizing a set of probes with the...

20060019271 - Submergence-induced protein-like factors: A pure polypeptide containing an amino acid sequence at least 80% identical to SEQ ID NO:5. The polypeptide, when expressed in a cell, increases susceptibility of the cell to hepatitis C virus infection....

20060019269 - Susceptibility gene for myocardial infarction, stroke, and paod, methods of treatment: Linkage of myocardial infarction (MI) and a locus on chromosome 13q12 is disclosed. In particular, the FLAP gene within this locus is shown by genetic association analysis to be a susceptibility gene for MI and ACS, as well as stroke and PAOD. Pathway targeting for treatment and diagnostic applications in...

20060019265 - Transmission-based luminescent detection systems: A luminescent detection system that employs transmission-based detection is provided for use with a chromatographic-based assay device. Unlike conventional systems, the detection system of the present invention is portable, simple to use, and inexpensive. For example, the system may be selectively controlled to reduce reliance on expensive optical components, such...

20060019281 - Tumor suppressor car-1: The present invention relates to a new tumor suppressor, designated CAR-1, the gene for which is located on the short arm of human chromosome 1. This gene is directly implicated in colon, kidney and breast cancers, and the CAR-1 ubiquitous expression of the corresponding transcript suggests that it may be...

20060019294 - Tyrosine kinase substrate (tks) proteins: The present invention relates to novel Tks 107, Tks 113, Tks 118 and Tks 202 polypeptides, nucleotide sequences encoding polypeptides, as well as various products and methods useful for the diagnosis and treatment of various related diseases and conditions....

20060019316 - Apparatus and method for automated protein design: The present invention relates to apparatus and methods for quantitative protein design and optimization....

20060019308 - Cd38 modulated chemotaxis: The present invention relates to methods for modulating the migratory activity of cells expressing CD38 for the treatment of disorders including, but not limited to, inflammation, ischemia, asthma, autoimmune disease, diabetes, arthritis, allergies, infection with pathogenic organisms, such as parasites, and transplant rejection. Such cells include, for example, neutrophils, lymphocytes,...

20060019310 - Cell regulatory genes, encoded products, and uses related thereto: Disclosed herein are methods for diagnosing cancer in a biological sample comprising measuring the level of a p63 gene product in the sample....

20060019312 - Cis-interactions between mhc molecules and co-expressed proteins, related neurological and non-neurological disorders and related therapeutic/diagnostic utilities: The present invention relates to the interactions between MHC Class I molecules and Co-expressed Proteins within the same cell (i.e., cis interaction). These interactions affect overt functional cellular processes by way of influencing protein trafficking and/or signal transduction. For example, in the nervous system, MHC Class I/Co-expressed Protein Interactions can...

20060019314 - Increasing life span by modulation of smek: The Smek (Suppressor of mek null) gene is described and characterized. Smek acts in the stress response pathway of animals by binding to and enhancing the transcription of FOXO, thereby providing the link between the stress response pathway and the insulin/IGF-1 pathway. Given the link between both the stress response...

20060019307 - Interleukin-8 homologous polypeptides and therapeutic uses thereof: The present invention is directed to novel polypeptides having structural homology to IL-8 and to nucleic acid molecules encoding those polypeptides. Also provided herein are vectors and host cells comprising those nucleic acid sequences, chimeric polypeptide molecules comprising the polypeptides of the present invention fused to heterologous polypeptide sequences, antibodies...

20060019313 - Method for detecting molecular surface interactions: A method for determining interaction of an analyte with a binding agent immobilized to a solid support surface by contacting the surface with a fluid sample containing the analyte, and detecting binding events at the solid support surface, wherein the sample is based on a complex medium containing at least...

20060019315 - Multimarker panel for diabetes type 1 and 2: The present invention relates to a method and means for diagnosing or risk stratification of co-morbidities, particularly cardiovascular complications in diabetes patients. The invention particularly relates to a method for diagnosing whether a diabetes patient is suffering from a cardiovascular complication or is at risk of suffering from a cardiovascular...

20060019311 - Process for detecting prp using a macrocyclic adjuvant ligand: A process for detecting the forms of the prion pathogens responsible for subacute, transmissible, spongiform encephalopathies, including a macrocyclic adjuvant ligand (AML), free or bound to a support, that is added to a biological sample capable of containing PrPsc, the resulting suspension then being reacted with an anti-PrPsc antibody, and...

20060019309 - Self-assembling peptide surfaces for cell patterning and interactions: This invention describes self assembled monolayers (SAMs) manufactured by imprinting reactive peptides onto solid supports. The invention further relates to methods of preparing and using these improved SAMs....

20060019317 - Assay systems and methods for detecting molecules that interact with membrane channels: The invention provides an assay system for detecting a molecule that interacts with a membrane channel, the assay system including cell membranes including one or more membrane channels; support bodies including scintillant and a coupling agent that associates with the cell membrane; a ligand that is selected to bind the...

20060019318 - Conformational and topological protein regulation: Methods and compositions are provided for identifying novel conformers of proteins not known to exist as different conformers. By using chimeric genes replacing a native signal sequence with a different signal sequence resulting in the production of a conformer, one can compare the native protein with the product of the...

20060019319 - Methods and apparatuses for conducting assays: Disclosed are methods for conducting assays of samples, such as whole blood, that may contain cells or other particulate matter. Also disclosed are systems, devices, equipment, kits and reagents for use in such methods. One advantage of certain disclosed methods and systems is the ability to rapidly measure the concentration...

20060019322 - Compositions and methods relating to prostate specific genes and proteins: The present invention relates to newly identified nucleic acids and polypeptides present in normal and neoplastic prostate cells, including fragments, variants and derivatives of the nucleic acids and polypeptides. The present invention also relates to antibodies to the polypeptides of the invention, as well as agonists and antagonists of the...

20060019321 - Planar optical waveguide based sandwich assay sensors and processes for the detection of biological targets including early detection of cancers: An assay element is described including recognition ligands adapted for binding to carcinoembryonic antigen (CEA) bound to a film on a single mode planar optical waveguide, the film from the group of a membrane, a polymerized bilayer membrane, and a self-assembled monolayer containing polyethylene glycol or polypropylene glycol groups therein...

20060019320 - Wnt mediated erbb signalling, compositions and uses related thereto: The present invention makes available assays and reagents inhibiting ErbB signals produced by Wnt protein comprising contacting a cell expressing ErbB and Wnt receptor (Frizzled) with a Wnt antagonist in a sufficient amount to reduce the sensitivity of the cell to ErbB signaling....

20060019324 - Pknb kinase and pstp phosphatase and methods of identifying inhibitory substances: The present invention relates to a pknB kinase and a pstP phosphatase as well as their use for identifying antibacterial substances....

20060019323 - Recombinant adenylate cyclase of bordetella sp. for diagnostic and immunomonitoring uses, method of diagnosing or immunomonitoring using said recombinant adenylate cyclase, and kit for diagnosing or immunomonitoring comprising said recombinant adenylate c: Diagnostic testing and immunomonitoring that uses genetically detoxified Bordetella pertussis CyaA as a delivery system are effective in tracking any immune responses, such as those generated by infectious and non-infectious diseases, or vaccinations, for example. T cells previously stimulated by a given antigen can be restimulated in vitro by the...

20060019325 - Screening assay: Screening assays for identifying an agent that modulates the activity of a phosphate catalyzing enzyme....

20060019326 - Use of three-dimensional microfabricated tissue engineered systems for pharmacologic applications: The present invention generally relates to a combination of the fields of tissue engineering, drug discovery and drug development. It more specifically provides new methods and materials for testing the efficacy and safety of experimental drugs, defining the metabolic pathways of experimental drugs and characterizing the properties (e.g., side effects,...

20060019327 - Transcutaneous analyte sensor: e

20060019328 - Glucose dehydrogenase: Modified water-soluble glucose dehydrogenases having pyrrolo-quinoline quinone as a coenzyme are provided wherein at least one amino acid residue is replaced by another amino acid residue in a specific region. Modified water-soluble PQQGDHs of the present invention have improved affinity for glucose....

20060019329 - Method of screening for protein inhibitors and activators: Chemical agents which are inhibitors or activators of a protein whose expression affects a phenotypic characteristic of the cell, especially a cultural or morphological characteristic, can be identified by their more pronounced effect on cells producing higher, usually non-naturally occurring, levels of the protein, than on cells producing little or...

20060019331 - Method and system for generating a telephone alert indicating the presence of an analyte: A system is provided for detecting a presence or amount of an analyte in a sample. The system may include a detection device detecting a characteristic of a fluid in communication with the sample to generate an electrical signal corresponding to the characteristic of the fluid and a processor coupled...

20060019330 - Staphylococcus detection: The invention relates to methods of detecting staphylococcus....

20060019332 - Deparaffinization compositions for dewaxing tissue specimens: Compositions and methods are provided for dewaxing wax-embedded biological specimens prior to histochemical analysis. The compositions and methods provided can effectively remove wax or improved wax-based embedding materials, particularly paraffin-based, from specimens during preparation for histochemical or other diagnostic analyses, while minimizing danger to users, achieving compatibility with automated use,...

20060019333 - Control of reactor environmental conditions: The present disclosure generally relates to chemical, biological, and/or biochemical reactor chips and/or reaction systems such as microreactor systems, as well as methods for constructing and using such systems. In some cases, humidity control materials are utilized to provide beneficially high rates of gas exchange. The humidity control materials may...

20060019334 - Production of flavonoids by recombinant microorganisms: Methods and compositions are provided for production of flavonoids in microbial hosts. The compositions comprises a set of genes which encode for enzymes involved in one or more steps in the biosymthetic pathway for the conversion of phenylpropanoids to various flavonoids. The method comprises the steps of introducing the set...

20060019341 - Aminopeptidase: A gram-negative bacterial cell is described that is deficient in a chromosomal gene present in a wild-type such cell which gene shares at least 80% sequence identity with the native sequence of the yfcK gene and encodes an aminopeptidase. Alternatively, a gram-negative bacterial cell is deficient in a chromosomal gene...

20060019347 - Biosynthetic polypeptides utilizing non-naturally encoded amino acids: Modified biosynthetic polypeptide molecules, methods for manufacturing, and uses thereof are provided....

20060019344 - C-erbb-2 external domain: gp75: Disclosed are methods and compositions for identifying malignant tumors that overexpress the c-erbB-2 oncogene. Assays useful for diagnosis and prognosis of neoplastic disease are provided which detect the external domain of c-erbB-2, the glycoprotein gp75 and quantitate the level of gp75 in the biological fluids of mammals carrying a tumor...

20060019340 - Cd44 polypeptides, polynucleotides encoding same, antibodies directed thereagainst and method of using same for diagnosing and treating inflammatory diseases: An isolated polypeptide is provided. The isolated polypeptide comprising an antigen recognition domain capable of specifically binding a CD44 polypeptide as set forth in SEQ ID NO: 2 and incapable of binding a CD44 polypeptide selected from the group consisting of: SEQ ID NO: 4 or 6....

20060019335 - Compositions and methods to promote c-iap autodegradation: The present invention generally relates to a method that may be employed as a means to regulate apoptosis. More particularly, the current invention relates to the use of Smac nucleic acid sequences and amino acid sequences expressed therefrom, which promote the ubiquitylation-mediated, auto-degradation of c-IAP....

20060019337 - Dual expression vector system and screening methods: The present invention features vectors that contain a promoter effective for expression in bacterial cells and a promoter effective for expression in insect cells. The dual promoter system allows use of the same vector in both host cell systems so that construction of only a single vector is needed to...

20060019336 - Factor vii or viia - like molecules: Conjugates of Factor VII (FVII) and Factor VIIa (FVIIA) are provided, as are methods for preparing them. Methods for producing novel polypeptides contributing to the production of such conjugates are provided. Methods of treatment by administering a FVII or FVIIa conjugate are provided....

20060019345 - Human gabab receptor 1 promoters: The present invention relates to nucleic acid molecules constituting GABAB receptor 1 promoters P1a and/or P1b, and to methods for screening for compounds which are modulators of GABAB receptor 1 transcription, said methods comprising the use of nucleic acid molecules constituting GABAB receptor P1a and/or P1b promoters....

20060019346 - Identification of a novel bitter taste receptor t2r76 that specifically responds to brucine and prop bitter ligands: Isolated nucleic acids encoding T2R76 polypeptides, recombinantly expressed T2R76 polypeptides, heterologous expression systems for recombinant expression of T2R76 polypeptides, assay methods employing the same, and methods for altering taste perception via administration of a T2R76 modulator. These T22R76 polypeptides can be expressed alone or co-expressed with another T2R polypeptide, preferably...

20060019342 - Increasing the production of recombinant antibodies in mammalian cells by site-directed mutagenesis: The present invention relates to a reliable, reproducible method for improving the producibility of an antibody. More specifically, this invention provides a method for modifying the heavy chain of an antibody to improve its producibility in eukaryotic cells. Additionally, the method of the invention may improve both antibody producibility and...

20060019339 - Measurement of bioactive hepcidins: The present invention concerns a method for the oxidative refolding of a hepcidin polypeptide to a form that is mature, bioactive and folded as in the native configuration and molecular mass; a method for measuring the level of native, bioactive hepcidin in a vertebrate animal; a method for measuring the...

20060019348 - Method of mass producing silk protein and gene recombinant silk-like protein with added functionality: A method of producing silk or silk-like protein wherein silk or a silk-like polymer comprising at least one protein selected from among domestic silkworm fibroin, wild silkworm fibroin, elastin and fibronectin, and essentially comprising the aforesaid domesticated silkworm fibroin or silkworm fibroin is designed. The minimum unit of the silk...

20060019343 - Pichia methanolica secretory signal: Novel Pichia methanolica secretory signal polypeptides, polynucleotides encoding the polypeptides, and related compositions and methods of using are disclosed. Methods of producing large amounts of recombinant proteins by employing DNA constructs having a polypeptide of interest preceded by a novel Pichia methanolica secretory signal sequence....

20060019349 - Position dependent recognition of gnn nucleotide triplets by zinc fingers: The specificity of binding of a zinc finger to a triplet or quadruplet nucleotide target subsite depends upon the location of the zinc finger in a multifinger protein and, hence, upon the location of its target subsite within a larger target sequence. The present disclosure provides zinc finger amino acid...

20060019338 - Primate prokineticin and prokineticin receptor polypeptides, related compositions and methods: The invention provides an isolated squirrel monkey prokineticin receptor 2 (PKR2) polypeptide containing the amino acid sequence referenced as SEQ ID NO:2; an isolated chimpanzee PKR2 containing the amino acid sequence referenced as SEQ ID NO:4; and an isolated rhesus monkey prokineticin receptor 1 (PKR1) referenced as SEQ ID NO:30....

20060019350 - Recombinant negative strand rna virus expression system and vacccines: Recombinant negative-strand viral RNA templates are described which may be used with purified RNA-directed RNA polymerase complex to express heterologous gene products in appropriate host cells and/or to rescue the heterologous gene in virus particles. The RNA templates are prepared by transcription of appropriate DNA sequences with a DNA-directed RNA...

20060019351 - Enhanced secretion of a polypeptide by a microorganism: Described herein are methods for the enhanced production of secreted proteins. The secretion of a protein of interest having a substantially non-polar carboxy tail is enhanced by the placement of charged amino acid residues at the carboxy terminus either by adding to the native peptide or by replacing, i.e., substituting,...

20060019352 - Methods of increasing the production of cobalamins using cob gene expression: Novel polypeptides involved in the biosynthesis of cobalamines and/or cobamides, in particular coenzyme B12, genetic material responsible for expressing these polypeptides, and a method for preparing them, are described. A method for amplifying the production of cobalamines, and particularly coenzyme B12, using recombinant DNA techniques, are also described....

20060019353 - Protein-nucleic acid conjugate for producing specific nucleic acid: This invention provides inter alia an in vitro process for producing multiple specific nucleic acid copies in which the copies are produced under isostatic conditions, e.g., temperature, buffer and ionic strength, and independently of any requirement for introducing an intermediate structure for producing the copies. In other aspects, the invention...

20060019354 - Compositions and methods of using a regulator of biopolymer production: The present invention provides nucleic acid sequences and variants thereof capable of modulating exopolysaccharide production in Sphingomonas, and provides methods of using such nucleic acid sequences to generate bacteria that hyper-produce exopolysaccharide in slime form....

20060019355 - L-amino acid-producing microorganism and method for producing l-amino acid: L-amino acids are produced by culturing a microorganism which has an ability to produce the L-amino acid, but has been modified so that expression of the ybjE gene has been enhanced. The L-amino acid is collected from the culture medium or from the microorganism....

20060019356 - Polynucleotides encoding polypeptides involved in intermediates metabolism of the central metabolic pathway in methylophilus methylotrophus: The present invention provides polypeptides and polynucleotides involved in central intermediates metabolism in Methylophilus methylotrophus and methods of producing amino acids in microorganisms having enhanced or attenuated expression of these polypeptides and/or polynucleotides....

20060019357 - Nucleotide sequences which code for the rpsl gene: An isolated polynucleotide comprising a polynucleotide sequence chosen from the group consisting of a) polynucleotide which is identical to the extent of at least 70% to a polynucleotide which codes for a polypeptide which comprises the amino acid sequence of SEQ ID No. 2, b) polynucleotide which codes for a...

20060019358 - Process for the preparation of fexofenadine:

20060019359 - Method for producing optically active 1,2-diols by microorganism cuturing: wherein R is a methyl, ethyl, propyl, chloromethyl, or hydroxyethyl group, in a culture medium whose Ca2+ concentration at the beginning of culturing is controlled and which contains a racemic mixture of the compound as a carbon source; and a second step of recovering the optically active compound remaining in...

20060019360 - Process for producing ethanol: A process for producing ethanol including a combination of biochemical and synthetic conversions results in high yield ethanol production with concurrent production of high value coproducts. An acetic acid intermediate is produced from carbohydrates, such as corn, using enzymatic milling and fermentation steps, followed by conversion of the acetic acid...

20060019361 - Immobilization of cells in a matrix formed by biocompatible charged polymers under laminar flow conditions: This invention relates to a method for immobilizing cells under laminar flow conditions in a matrix formed by biocompatible charged polymers, to a flow device for the use with the method of the present invention, and to uses of the polymer matrices containing the cells immobilized using the method of...

20060019362 - Encapsulation of cells in biologic compatible scaffolds by coacervation of charged polymers: This invention relates to a method for the encapsulation of cells in biologic compatible three dimensional scaffolds and the use of such cells encapsulated in a scaffold. The cells are embedded in a charged polymer that is complex coacervating with an oppositely charged polymer within biologic compatible scaffolds. The polymer...

20060019364 - Acetyl coa carboxylase 2 sequences and methods: The present invention relates generally to novel nucleotide and amino acid sequences, and more particularly to novel human acetyl CoA carboxylase 2 (ACC2) and rat ACC2 sequences. The sequences provided herein can be expressed in a recombinant format. Methods of isolating the ACC2 sequence are also provided, which can be...

20060019363 - Cytosolic phospholipase a2-beta enzymes: The invention provides a novel calcium-independent cytosolic phospholipase A2-Beta enzyme, polynucleotides encoding such enzyme and methods for screening unknown compounds for anti-inflammatory activity mediated by the arachidonic acid cascade....

20060019365 - Ikk-alpha proteins, nucleic acids and methods: The invention provides methods and compositions relating to an IκB kinase, IKK-α, and related nucleic acids. The polypeptides may be produced recombinantly from transformed host cells from the disclosed IKK-α encoding nucleic acids or purified from human cells. The invention provides isolated IKK-α hybridization probes and primers capable of specifically...

20060019366 - Reversibly modified thermostable enzyme compositions and methods of making and using the same: The present invention provides reversibly modified thermostable enzyme compositions and methods for making the same. The present invention also provides methods of using the reversibly modified thermostable enzyme compositions, as well as kits and systems comprising the reversibly modified thermostable enzymes....

20060019367 - Method of producing microbial transglutaminase: The present invention provides a neutral metalloprotease from actinomycetes which selectively cleaves a pro-structure part of a microbial protransglutaminase and a gene encoding said neutral metalloprotease. An active microbial transglutaminase having the pro-structure part cleaved can be obtained by culturing a microorganism into which a gene encoding the neutral metalloprotease...

20060019368 - Constructs for homogenously processed preparations of beta site app-cleaving enzyme: The present invention is directed to engineered polypeptides having BACE activity. In certain embodiments, the polypeptides also comprise an engineered cleavage site. Also provided are polypeptides comprising a prodomain, an engineered cleavage site, and a protease domain; the polypeptides are properly folded and are cleaved at the engineered cleavage site...

20060019369 - Recombinant vector containing infectious human cytomegalovirus genome with preserved wild-type characteristics of clinical isolates: A recombinant vector containing infectious genome of human cytomegalovirus (HCMV) and being useful for the production of reconstituted HCMV virus retaining phenotypic characteristics of a clinical virus isolate including the ability to grow on endothelial cells and to induce microfusion is characterized in that it is obtainable by inserting DNA...

20060019370 - Phage-resistant microorganisms and genetic determinants of phage resistance: There are described new bacterial strains, plasmids derived thereof, a gene sequence included in the plasmids encoding a phage resistance system protein, and a method to confer phage resistance to microorganism cultures....

20060019371 - Macrolide analogs: Combinatorial libraries of polyketides can be obtained by suitable manipulation of a host modular polyketide synthase gene cluster such as that which encodes the PKS for erythromycin. The combinatorial library is useful as a source of pharmaceutically active compounds. In addition, novel polyketides and antibiotics are prepared using this method....

20060019373 - Biosensor with rf signal transmission: A device (1) and method for measuring and or detecting the presence of biomolecules. The device comprises a resonance circuit arranged to operate and emit a resonance frequency (f). The resonance circuit comprises or is coupled to a sensor element (5) for detecting the binding of biomolecules (6a) to binding...

20060019372 - Laser system: A laser system and interferometer are disclosed comprising a laser source for generating a laser beam, and first and second adjustable elements wherein the first and second adjustable elements have limited rotational motion so rotation of the first adjustable element causes deviation of a laser beam in one plane and...

20060019374 - Optical biosensor: An optical biosensor has a total reflection plate so as to totally internally reflect and transmit an incident light, a first grating and a second grating disposed separately on the total reflection plate, and a sensing membrane that is containing an enzyme and a chromogenic reagent and is sandwiched by...

20060019375 - Device for culturing cells, particularly human or animal cells: The invention relates to a device (30) for culturing cells of the most diverse type, particularly human or animal cells. According to the invention, a culture is prepared from cells of at least one specified type in a defined environment, and the cells of the relevant culture are supplied with...

20060019376 - Fermentation chamber and mixing apparatus: The claimed invention provides a fermentation chamber that is inexpensive to produce and use and is capable of providing sufficient agitation of the liquid medium to ensure proper mixing and/or aeration without damaging delicate components. The invention further provides a mixing apparatus for agitating one or more such fermentation chambers....

20060019377 - Flask: The present invention provides a flask having a top piece 20 and a bottom piece 22 with optical properties on the interior surface 24 of which cells are grown. The top piece 20 and the bottom piece 22 are joined by a removable elastomeric seal 26. By disengaging the elastomeric...

20060019378 - Cell culture device and manufacturing method thereof: A low-cost cell culture device with low cytotoxicity and improved cell adhesion is described, manufactured by a simplified coating process. At least the portion for cell culture on the surface of the cell culture device is coated with at least one of ε-poly-L-lysine, further polymerized derivatives of ε-poly-L-lysine and their...

20060019379 - Apparatus and method for cell disruption: An apparatus for disrupting cells or viruses comprises a container having a chamber for holding the cells or viruses. The container includes at least one flexible wall defining the chamber. The apparatus also includes a transducer for impacting an external surface of the flexible wall to generate pressure waves in...

20060019380 - Genetic vaccines directed against bacterial exotoxins: The invention provides a gene transfer vector comprising a humanized nucleic acid sequence encoding an immunogenic portion of one or more exotoxins of Bacillus anthracis and a heterologous sorting signal. The invention also provides a method of producing an immune response against Bacillus anthracis in a host comprising administering to...

20060019381 - Hyperthermic inducible expression vectors for gene therapy and methods of use thereof: Methods and compositions are provided for transgene expression in target cells. Expression constructs using an inducible amplification system to drive expression of a therapeutic gene or other gene of interest in mammalian host cells are provided, as well as methods therefor. Inducible expression of the transgenes at high levels under...

20060019382 - Transcriptionally-activated aav inverted terminal repeats (itrs) for use with recombinant aav vectors: This invention provides transcriptionally-activated AAV ITRs (inverted terminal repeats) which are small and transcriptionally active and uses thereof to optimize the expression of relatively large transgenes packaged in recombinant AAV vectors....

20060019383 - Chemical inhibitors of mismatch repair: Dominant negative alleles of human mismatch repair genes can be used to generate hypermutable cells and organisms. By introducing these genes into cells and transgenic animals, new cell lines and animal varieties with novel and useful properties can be prepared more efficiently than by relying on the natural rate of...

20060019385 - Apparatus and methods for producing and using high-density cells and products therefrom: Disclosed and claimed is apparatus and methods for the growth of cells to high density, products therefrom and uses thereof. Also disclosed and claimed is the use of this method for the growth to high-density insect cells, such as the Spodoptera frugiperda Sf900+ cell line (ATCC: CRL 12579). Further disclosed...

20060019384 - Clonal cell lines derived from bti-tn-5b1-4: Improved insect cell lines, two of which are designated H5CL-B and H5CL-F (both of which are derived from the parental cell line BTI-TN-5B1-4, ATCC CRL 10859), possess the properties of increased production of baculovirus particles, increased expression of foreign proteins using a baculovirus expression system, and increased resistance to cell...

20060019386 - Protein domain related to deafness, osteoarthritis and abnormal cell proliferation: The present invention relates to genetic diagnosis and therapy of diseases of the nervous system (NS). More particularly, it relates to methods to induce neural precursor cells (NPCs) and to the identification of a domain that determines the functionality of polypeptides belonging to the atonal family and its use in...

20060019387 - Liver stem cell: The invention provides a primary liver stem cell and a cell doublet consisting of a hepatocyte and the stem cell, both of which are derived from normal liver tissue. Methods of isolating the cells, genetically altering the cells, and using the cells for transplantation are also within the invention....

20060019388 - Bioreactor for growing cell or tissue cultures: The invention relates to a bioreactor comprising a chamber for containing cells or tissue cultures within a culture medium. The bioreactor also comprises a detector capable of detecting a change in one or more metabolites associated with growth of the cell or tissue cultures within the chamber and a chamber...

20060019389 - Porous plasma protein matrices and methods for preparation thereof: The present invention relates to porous freeze-dried plasma protein matrices having an open channel structure and a concentration gradient of plasma proteins crosslinked by the action of thrombin, and methods of producing said matrices. The compositions of the present invention are useful clinically, per se or as cell-bearing implants....

20060019390 - Patterning substrate and cell culture substrate: The present invention intends primarily to provide such as a cell culture patterning substrate that is used to adhere cells in a highly precise pattern on a base material to culture and a cell culture substrate on which cells is adhered in a highly precise pattern. To attain the above-mentioned...

20060019391 - Liquid-gas-phase exposure reactor for cell culturing: Initiation of growth and cultivation of cells can be performed by introducing the cells into culture compartments of a liquid-gas-phase exposure bioreactor containing a supply chamber in which there are disposed hollow-filament membranes having an inside diameter of no larger than 5 mm, wherein an inner volume of said hollow-filament...

20060019392 - Novel bone mineralization proteins, dna, vectors, expression systems: The present invention is directed to isolated nucleic acid molecules that encode LIM mineralization protein, or LMP. The invention further provides vectors comprising nucleotide sequences that encode LMP, as well as host cells comprising those vectors. Moreover, the present invention relates to methods of inducing bone formation by transfecting osteogenic...

20060019394 - Bovine immunodeficiency virus (biv) based vectors: This invention pertains to BIV constructs encompassing BIV combination vectors, BIV vectors and BIV packaging vectors and particularly the invention pertains to a three vector system comprising: a) a BIV vector construct including a DNA segment from a BIV genome, a packaging sequence to package RNA into virions; a promoter...

20060019395 - Lentiviral vectors and uses thereof: The invention relates to compositions containing polynucleotide vectors capable of expressing a nucleic acid encoding a fusion polypeptide on the surface of a viral particle and/or a eukaryotic cell....

20060019393 - Minimal lentiviral vector system: A lentiviral vector system is described. The system comprises a lentiviral transfer vector and a packaging construct. The transfer vector comprises (a) a 5′ LTR; (b) a 3′ LTR comprising a polyadenylation signal; (c) a minimal packaging signal, (d) (i) at least one heterologous upstream enhancer (UE) sequences, and/or (ii)...

20060019396 - Retroviral vectors with enhanced efficiency of transgene expression and safety: The present application discloses a MoMSV/MoMLV hybrid-based retroviral vector, wherein gag and pol genes are completely removed....

20060019397 - Self-improving classification system: A self-improving classification system classifies specimens based on class identifiers. The system stores specimen profiles in a database that is updated with additional specimen profiles and with follow-up data that corrects classification of specimens that were initially incorrectly classified. Algorithms use the updated database to discover new class identifiers, modify...

20060014134 - Low-temperature storage of suspension samples in suspended sample chambers: A cryostorage device (100) is described that includes at least one data storage device (200) and at least one sample receptacle device (300) with at least one sample chamber (301, 302, etc.) for the uptake of a suspension sample, the at least one sample chamber (301, 302, etc.) being connected...

20060014135 - Assay for distinguishing live and dead cells: Image analysis methods and apparatus are used for distinguishing live and dead cells. The methods may involve segmenting an image to identify the region(s) occupied by one or more cells and determining the presence of a particular live-dead indicator feature within the region(s). In certain embodiments, the indicator feature is...

20060014136 - Method of screening for protection from microbial infection: Screening assays that allow for the identification of agents that modulate host cell response to microbial infection. The methods of the invention provide the ability to measure the reduction in toxicity to the host cell, which is directly related to the ability of a compound to alter the cell's responses...

20060014137 - System for cell-based screening: The present invention provides systems, methods, screens, reagents and kits for optical system analysis of cells to rapidly determine the distribution, environment, or activity of fluorescently labeled reporter molecules in cells for the purpose of screening large numbers of compounds for those that specifically affect neurite outgrowth....

20060014142 - Compositions and methods for detection of hepatitis a virus nucleic acid: Nucleic acid oligomeric sequences and in vitro nucleic acid amplification and detection methods for detecting the presence of HAV RNA sequences in samples are disclosed. Kits comprising nucleic acid oligomers for amplifying and detecting HAV nucleic acid sequences are disclosed....

20060014139 - Five-helix protein: Five-Helix protein, which comprises the three N-helices and at least two, but not three, of the three C-helices of the trimer-of-hairpin structure of HIV gp41, separated by linkers, such as amino acid residue linkers, is disclosed. Six-Helix protein, which includes the three N-helices and the three C-helices of the trimer-of-hairpin...

20060014141 - Method of detecting and quantifying cytomegalovirus: Combinations of oligonucleotide primers which allow specific amplification of the mRNA of the CMV β2.7 gene, RNA amplification using these combinations of oligonucleotide primers and a detection method which uses a fluorescent intercalative dye-labeled oligonucleotide probe to monitor the RNA amplification....

20060014140 - Molecular methods and compositions for detecting and quantifying respiratory viruses: The present invention relates to new methods and nucleic acid sequences for the detection and quantification of human metapneumovirus....

20060014138 - Phage microarray profiling of the humoral response to disease: The present invention relates to compositions and methods for cancer diagnostics, including but not limited to, cancer markers. In particular, the present invention provides methods and compositions for phage microarray profiling of cancer (e.g., prostate, lung, or breast cancer). The present invention further provides novel markers useful for the diagnosis,...

20060014143 - Preparation of potent macrophage activating factors derived from cloned vitamin d binding protein and its domain and their therapeutic usage for cancer, hiv-infection and osteopetrosis: Vitamin D-binding protein (Gc protein) and its small domain (approximately ⅕ of the Gc peptide also known as domain III) were cloned via a baculovirus vector. The cloned Gc protein and the cloned domain (Cd) peptide were treated with immobilized β-galactosidase and sialidase to yield macrophage activating factors, GcMAFc and...

20060014167 - Amplification of trace amounts of nucleic acids: Methods of reducing background during amplification of small amounts of nucleic acids employ careful analysis of sources of low level contamination. Ultraviolet light can be used to reduce nucleic acid contaminants in reagents and equipment. “Primer-dimer” background can be reduced by judicious design of primers. We have shown clean signal-to-noise...

20060014191 - Analog probe complexes: The present invention relates to the detection of target sequences. The present description discloses compositions and methods involving analog nucleic acids, such as PNA and L-DNA, for the detection of nucleic acids. Additionally, hybrid detectable markers are provided....

20060014200 - Apparatus for analysis of a nucleic acid amplification reaction: An apparatus for determining a threshold value (e.g., a threshold cycle number or a time value) in a nucleic acid amplification reaction comprises a detection mechanism for measuring, at a plurality of different times during the amplification reaction, at least one signal whose intensity is related to the quantity of...

20060014172 - Aptamer-nanoparticle conjugates and method of use for target analyte detection: The present invention provides aptamer probes, nanoparticle-aptamer conjugate probes, aptamer arrays, methods of detecting target analytes in a sample comprising detecting binding of a target analyte with aptamer probes, method of detection, and kits....

20060014187 - Association of single nucleotide polymorphisms in ppargamma with osteoporosis: The current invention is based on the discovery that the Prol2Ala and VN102 single nucleotide polymorphisms in the PPARγ2 provides a method of determining a susceptibility to osteoporosis by detecting the presence of PPARγ of the alleles....

20060014161 - Combination comprising biochip and optical detection device: The invention relates to a method and apparatus for the routine analysis of samples that contain biomolecules. The invention uses biochips or arrays in the format of conventional 35 mm film slides. These film slide arrays allow employing commercially available detection, storage and processing equipment. The use of array technology...

20060014164 - Computer software to assist in identifying snps with microarrays: The present invention is a method to assist in the identification of single nucleotide polymorphisms (SNP) from microarray hybridization data. Data from hybridization protocols run on microarrays often have variations in the data resulting from variations in hybridization conditions and efficiencies and variations in optical intensities. An algorithm is described...

20060014189 - Controls for determining reaction performance in polynucleotide sequence detection assays: The present teachings relate to methods, compositions, and kits for detecting one or more target polynucleotide sequences in a sample. In some embodiments of the present teachings, probes are hybridized to complementary target polynucleotides and are ligated together to form a ligation product. Some embodiments of the present teachings comprise...

20060014194 - Cyclic peptides for treatment of cachexia: e

20060014181 - Detection of dna mismatches and oxidative lesions: The present invention describes methods for directly labeling the 3′-phosphate end at a nucleotide site. Further, as internal 3′-phosphate termini on DNA duplexes are also associated generally with oxidative lesions, these methods provide a general strategy for labeling, and therefore, detecting the frequency of oxidative DNA lesions. The present invention...

20060014150 - Development of therapeutics for the treatment of endotoxin-meidated diseases: The subject invention comprises a method for identifying an evolutionarily meaningful nucleotide change in a primate's TLR4 polynucleotide. It further comprises methods for identifying agents that interact with the corresponding evolutionarily meaningful amino acid change so as to modulate the function of the TLR4 polypeptide, thereby attenuating activation of the...

20060014147 - Diagnostics and therapeutics for diseases associated with oxytocin receptor (oxtr): The invention provides a human OXTR which is associated with the hematological diseases, cardiovascular diseases, disorders of the peripheral and central nervous system, cancer diseases, respiratory diseases, metabolic diseases. The invention also provides assays for the identification of compounds useful in the treatment or prevention of hematological diseases, cardiovascular diseases,...

20060014163 - Dna-binding polymers: Methods and compositions are provided for forming complexes between dsDNA and novel DNA-binding polymers comprising N-terminal thiophene-containing moieties which exhibit selectivity for T-A base pairs. By appropriate choice of target sequences and DNA-binding polymers, complexes comprising polymer-DNA are obtained with high association constants. The formation of complexes can be used...

20060014202 - Electrical connection structure, production method thereof, and electric wiring method: An electrical connection structure that is able to electrically connect wiring to a biopolymer, a production method of the electrical connection structure, and an electric wiring method which is able to perform wiring on a nanometer-scale. A first aspect of the production method of the present invention uses a carbon...

20060014203 - Electrical connection structure, production method thereof, and electric wiring method: An electrical connection structure that is able to electrically connect wiring to a biopolymer, a production method of the electrical connection structure, and an electric wiring method which is able to perform wiring on a nanometer-scale. A first aspect of the production method of the present invention uses a carbon...

20060014183 - Extendable probes: The invention relates to probes which are extendable useful as PCR probes and in probe libraries. The invention further relates to prevention of replication of a primer extension product in PCR reactions....

20060014175 - Functionalized fluorescent nanocrystal detection system: The present invention include fluorescent nanocrystals which have high fluorescence intensity, are water soluble, exhibit physical and chemical stability, and whose spectral properties are detectably modified as the size of functional groups bonded to the nanocrystal surface change when contacted with target molecules in a sample. The molecules in the...

20060014180 - Human phosphatase ret31, and variants thereof: The present invention provides novel polynucleotides encoding human phosphatase polypeptides, fragments and homologues thereof. Also provided are vectors, host cells, antibodies, and recombinant and synthetic methods for producing said polypeptides. The invention further relates to diagnostic and therapeutic methods for applying these novel human phosphatase polypeptides to the diagnosis, treatment,...

20060014162 - Identification and characterization of racemases, definition of protein signatures, and a test for detecting d-amino acid and for screening molecules capable of inhibiting the activity of racemase, especially proline racemase: This invention provides identification and characterization of racemases and definition of protein signatures of these racemases. This invention also provides identification of nucleic acid molecules encoding a peptide consisting of a motif characteristic of the protein signatures, and to the peptides consisting of these motifs. Antibodies specific for the peptides...

20060014148 - Igg fc/hiv-gp120/c3d fusion protien: The present invention relates, in general, to an immunogen and, in particular, to an immunogen for inducing antibodies that neutralize a wide spectrum of HIV primary isolates. The invention also relates to a method of inducing anti-HIV antibodies using same....

20060014197 - In vivo screening methods for identifying inhibitors of rna polymerases: In vivo screening methods for identifying inhibitors of RNA polymerase (RNAP) are provided by the present invention. In certain embodiments, methods according to the invention include steps of: (a) expressing a test RNAP in a cell containing a first reporter; (b) expressing a control RNAP in the cell of step...

20060014179 - Inferring function from shotgun sequencing data: Methods are described for detecting genes that encode toxic proteins using maps derived from shotgun libraries by determining the presence of gaps in clone start sites on either side of open reading frames. The method is exemplified by identifying a previously unknown restriction endonuclease gene....

20060014185 - Integrated nucleic acid analysis: The present invention relates to an integrated method of nucleic acid analysis, and more particularly to a simplified sample pre-treatment, which renders the method more easily automated, where the sample is provided on or applied onto a solid matrix and the subsequent amplification and detection steps are performed in one...

20060014195 - Investigation of dna samples: A method of investigating a DNA sample is provided involving taking at least two sub-samples from the sample, amplifying the sub-samples, analysing the sub-samples to obtain identity information and amount of that identity about one or more alleles indicated as present in the sub-samples in respect of 5 or more...

20060014198 - Method for apparatus for detecting luminescence light from a porous support structure: Method and apparatus for detecting chemical and/or biochemical reactions and/or bindings. A two-dimensional support structure which has, distributed over at least one surface region, a multiplicity of pores which extend continuously from one surface of the support structure to the opposite surface, is provided wherein the pores are bounded by...

20060014174 - Method for the detection and identification of circulating micrometastatic cancer cells: The invention relates to an method for the detection of cancer cells of epithelium origin expressing the Ep-Cam antigen and identification of circulating micrometastatic cancer cells in peripheral blood tissues and other body fluids of patients suffering from cancer of epithelial origin, using a sample of total blood, body fluids...

20060014146 - Method of creating a library of bacterial clones with varying levels of gene expression: The present invention relates to a method of creating DNA libraries that include an artificial promoter library and/or a modified ribosome binding site library and transforming bacterial host cells with the library to obtain a population of bacterial clones having a range of expression levels for a chromosomal gene of...

20060014168 - Method of diagnosis of inclusion body myopathy-paget bone disease-frontotemporal dementia syndrome: The present invention is based on the discovery of a genetic basis for inclusion body myopathy-Paget bone disease-frontotemporal dementia syndrome (IBMPFD). We have determined that genetic alterations in the gene encoding vasolin containing protein (VCP) is responsible for IBMPDF syndrome. In particular, we have identified six missense mutations within VCP...

20060014145 - Methods and articles for high throughput analysis of biomolecular interactions: Methods, compositions articles of manufacture, and kits for characterizing biomolecular interactions are provided. The interactions may involve various combinations of proteins, lipids, carbohydrates and small molecules. For example, interactions between polynucleotide segments and polynucleotide binding protein(s) may be characterized....

20060014182 - Methods and compositions for amplification of rna sequences: The invention provides methods for linear and exponential amplification of RNA. They are particularly suitable for amplifying a plurality of RNA species in a sample. The methods are based on hybridization of polynucleotide comprising a propromoter sequence to a primer extension product to generate an intermediate polynucleotide capable of driving...

20060014152 - Methods and reaction mixture reagent for increasing the specificity and fidelity of polymerase reactions: A method for reducing the efficiency of primer extension by polymerase enzymes when the 3′ end of a primer or growing nucleic acid chain does not hybridize perfectly with the target, for increasing the selectivity of single nucleotide mutation or gene analyses, for suppressing false positive results and for enhancing...

20060014190 - Methods for analyzing short tandem repeats and single nucleotide polymorphisms: Methods for genotyping a sample comprising nucleic acid are provided. The methods comprise analyzing STR and SNP loci....

20060014186 - Methods for genotype screening of a strain disposed on an adsorbent carrier: The present invention provides a method to rapidly provide genotype screening of a plurality of biological samples disposed on an adsorbent carrier in a designated well of a microwell container for remote user by a screening laboratory. Additionally, this invention relates to a genotyping kit including at least one swab...

20060014192 - Methods for genotype screening using magnetic particles: This invention relates to a method of an assay of genomic nucleic acid. Additionally, this invention relates to various methods to detect or screen for designated genetic sequences or portion thereof derived from a biological sample. More particularly, this invention relates to the use of magnetically responsive magnetic particles that...

20060014184 - Methods for identification, and compounds useful for the treatment of degenerative & inflammatory diseases: The present invention relates to in vivo and in vitro methods, agents and compound screening assays for inhibiting extra-cellular matrix degradation, including joint degenerative inhibiting and/or anti-inflammatory pharmaceutical compositions, and the use thereof in treating and/or preventing a disease involving extra-cellular matrix degradation in a subject....

20060014169 - Methods for obtaining directionally truncated polypeptides: Methods, compositions and kits are disclosed for obtaining directionally truncated polypeptides by inserting a transposon. Preferably the transposon comprises a selectable marker and an ori, and optionally a promoter, a ribosome binding site and a translation start codon, into a target sequence in vitro or in vivo. Amplification products, varying...

20060014154 - Methods for repairing degraded dna: The present invention provides methods and kits for repair of degraded DNA which may then be used as a template for efficient amplification by a number of different amplification reactions. The method relies upon a series of enzymatic activities provided by DNA repair enzymes....

20060014149 - Methods for rolling circle amplification and signal trapping of libraries: A method for isolating a polynucleotide that encodes a polypeptide of interest which comprises a signal sequence for secretion or partial secretion, utilizing rolling circle amplification and signal trapping of libraries....

20060014160 - Methods for the diagnosis and treatment of breast cancer: The present invention provides a method for diagnosing breast cancer in a subject. The present invention also provides a method for treating breast cancer in a subject. Finally, the present invention provides a method for assessing the efficacy of breast cancer therapy in a subject who has undergone or is...

20060014155 - Methods for the production of sensor arrays using electrically addressable electrodes: Methods for building sensor arrays using electrical signals to selectively functionalize individual electrodes in an array of electrically addressable electrodes are provided. These methods are useful for providing sensor arrays for use in chemical and biochemical assays. The method is based on the sequential electrochemical reduction of functional groups on...

20060014201 - Methods of and kits and compositions for diagnosing colorectal tumors and metastasis thereof: In vi tro methods of determining whether or not an individual has metastasized colorectal cancer cells are disclosed. In vitro methods of determining whether or not tumor cells are colorectal in origin are disclosed. In vitro kits for practicing the methods of the invention and to reagents and compositions useful...

20060014159 - Methods of determining precise herg interactions and designing compounds based on said interactions: The present invention discloses methods of determining highly precise interactions between the HERG ion channel and various compounds. The methods of the present invention utilize the nonsense codon suppression methods combined with heterologous in vivo expression in Xenopus oocytes....

20060014165 - Methods of diagnosis and treatment for asthma and other respiratory diseases based on haplotype association: Methods for diagnosis of asthma or a susceptibility to asthma based on detection of at-risk haplotypes associated with MAP3K9 are disclosed. Also methods for treatment of asthma or a susceptibility to asthma based on detection of at-risk haplotypes associated with MAP3K9 are disclosed. In particular, pathway targeting for treating individuals...

20060014204 - Methods of inducing formation of functional and organized lymphatic vessels: Methods of inducing formation of functional and organized lymphatic vessels are described. Specifically, the methods relate to using Tie2 agonists to induce formation of functional and organized lymphatic vessels. The methods also relate to treating defects, diseases, and disorders characterized by lymphatic vessel malfunction, disorganization, and damage....

20060014199 - Molecular detection of chromosome aberrations: The invention relates to the field of cytogenetics and the application of genetic diagnostic techniques in pathology and hematology. Specifically, the invention relates to nucleic acid probes that can be used in hybridization techniques for the detection of chromosomal aberrations and other gene rearrangements such as immunoglobulin and T-cell receptor...

20060014196 - Nanogripper device and method for detecting that a sample is gripped by nanogripper device: A fixed electrode and a movable electrode to be used to drive each arm are formed at a drive unit. As a voltage is applied between the fixed electrode and the movable electrode, the movable electrode is caused to move by coulomb force, thereby driving the arm 3 in a...

20060014166 - Novel nucleotide and amino acid sequences, and assays and methods of use thereof for diagnosis of endometriosis: Novel markers for endometriosis that are both sensitive and accurate. These markers are differentially expressed in endometriosis specifically, as opposed to normal tissue. The measurement of these markers, alone or in combination, in patient samples provides information that the diagnostician can correlate with a probable diagnosis of endometriosis. The markers...

20060014205 - Novel polymerase compositions and uses thereof: The subject invention provides novel compositions containing a mixture of (a) an enzyme that possesses substantial 3′-5′ exonuclease activity (b) a DNA polymerase with less 3′-5′ exonuclease activity than the enzyme with substantial 3′-5′ exonuclease activity. Preferably, the DNA polymerase for inclusion in the compositions are DNA polymerases that substantially...

20060014153 - Novel proteins and nucleic acids encoding same: Disclosed herein are nucleic acid sequences that encode novel polypeptides. Also disclosed are polypeptides encoded by these nucleic acid sequences, and antibodies, which immunospecifically-bind to the polypeptide, as well as derivatives, variants, mutants, or fragments of the aforementioned polypeptide, polynucleotide, or antibody. The invention further discloses therapeutic, diagnostic and research...

20060014156 - Nucleic acid detecting or quantifying processes: This invention provides novel compositions and processes for analyte detection, quantification and amplification. Nucleic acid arrays and libraries of analytes are usefully incorporated into such compositions and processes. Universal detection elements, signaling entities and the like are employed to detect and if necessary or desirable, to quantify analytes. Amplification of...

20060014193 - Nucleoside phosphoramidates and nucleoside phosphoramidases: The invention provides isolated nucleic acid sequences encoding polypeptides having nucleoside phosphoramidase activity, and methods of screening for nucleoside phosphoramidate compounds that are cleaved by a phosphoramidase or for phosphoramidases that are able to cleave phosphoramidate compounds. The invention also provides methods of delivering a nucleoside monophosphate....

20060014151 - Optical dna sensor, dna reading apparatus, identification method of dna and manufacturing method of optical dna sensor: The advantage is to provide a DNA reading apparatus which can sense fluorescence even if the sensitivity of a CCD image sensor or a photomul is low and can be constructed in a compact size. An optical DNA sensor having: a solid imaging device, and a plurality types of DNA...

20060014173 - Pr/set-domain containing nucleic acids, polypeptides, antibodies and methods of use: The present invention provides an isolated nucleic acid molecule encoding a PFM/SET polypeptide. Also provided is an isolated nucleic acid molecule encoding a functional fragment of a PFM/SET polypeptide that contains a PR, SET, PRAZ or PKZL domain of a PFM/SET polypeptide of the invention. Further provided by the invention...

20060014144 - Pseudonucleotide comprising an intercalator: The present invention relates to intercalator pseudonucleotides. Intercalator pseudonucleotides according to the invention are capable of being incorporated into the backbone of a nucleic acid or nucleic acid analogue and they comprise an intercalator comprising a flat conjugated system capable of co-stacking with nucleobases of DNA. The invention also relates...

20060014176 - Reactor for chemical synthesis: The present invention relates to the design and use of a multipurpose reactor that may be adapted to accommodate a wide variety of solid phase and liquid phase reaction chemistries....

20060014171 - Senp1 as a marker for cancer: The present invention provides methods of detecting cancer cells by detecting the quantity of SENP1 and/or telomerase in a sample....

20060014157 - Sensitivity test to predict efficacy of anti-cancer therapies: The present disclosure provides methods for determining the sensitivity of cancerous cells to various anti-cancer therapies and predicting the efficacy of these therapies. Specifically, the present disclosure provides methods for predicting the efficacy of one or more candidate anti-cancer therapies in a patient, based on determining the sensitivity of the...

20060014177 - Stable protein storage and stable nucleic acid storage in recoverable form: The invention provides compositions and methods for storage of biomolecules. The biomolecules are stored via absorption to a substrate. Absorbed biomolecules can be eluted or recovered from the substrate at a future time, and optionally be subjected to a subsequent analysis or application. Biomolecules absorbed to a substrate for storage...

20060014188 - System to detect inducible translocation: The present invention relates to a novel method for screening to identify/detect polypeptides capable of translocating into or out of the nucleus of a cell in response to induction by an external stimulus or stimuli. Kits for practicing the method of the invention are also encompassed by the present invention....

20060014158 - Use of a fibroblast growth factor-binding protein for the treatment and diagnosis of diabetic wound healing problems: The present invention relates to the use of fibroblast growth factor-binding protein (FGF-BP) polypeptides, and functional variants of these polypeptides, respectively, or of nucleic acids encoding these polypeptides or variants, or of functional variants of these nucleic acids, and/or of a cell which is expressing an FGF-BP polypeptide or functional...

20060014170 - Use of an extraction control in a method of extracting nucleic acids: The present invention relates to a method of ensuring the effectiveness of the extraction workup from a biological sample of nucleic acid. The inventive method is able to distinguish between possible defects in the extraction of nucleic acid from a sample and possible defects in a subsequent amplification step. The...

20060014178 - Use of mrf-2 for screening and therapies: Mrf-2 is essential for accumulation of lipid stores in postnatal life. Homozygous loss of the ARID gene Mrf-2 resulted in a high rate of neonatal mortality that was partially strain-dependent in mice. Loss of Mrf-2 expression did not affect embryonic survival, embryonic growth or birth weight. Lipid accumulation was severely...

20060014206 - Assay methods for state-dependent calcium channel agonists/antagonists: Methods of identifying activators and inhibitors of voltage-gated ion channels are provided in which the methods employ cells transfected with a voltage-gated ion channel of interest and a corollary channel to control the membrane potential of the cells by changing extracellular ion concentration. This allows for more convenient, more precise...

20060014211 - Detection of mesothelin-/megakaryocyte potentiating factor-related peptides for assessment of the peritoneum and the peritoneal cavity: The invention relates to methods and kits for assessing occurrence in patient mesothelial fluid of peptides having amino acid sequences related to those of mesothelin, megakaryocyte potentiating factor, and other peptides that have been associated with occurrence in the serum of mesothelioma patients. The methods and kits can be used...

20060014208 - Functional assays for identifying compounds that modulate t1r2/t1r3 (sweet) taste: Functional assays for identifying compounds that activate or modulate the activation of the T1R2/T1R3 (sweet) taste receptor are provided. These assays detect the effect of one or more compounds on the activation of T1R2/T1R3 (sweet) taste receptor or on the activation of T1R2/T1R3 sweet taste receptor by another, compound e.g.,...

20060014216 - Inhibition of tau-tau-association: A composition and use thereof for the prophylaxis or treatment of Alzheimer's disease, motor neurone disease, Lewy body disease, Pick's disease or progressive supranuclear palsy. The composition includes an agent which modulates or inhibits tau-tau association and which does not inhibit tau-tubulin binding. In the method the agent is administered...

20060014209 - Method for differentiation of alzheimer's disease into subgroups: A method for diagnosing distinct subgroups of Alzheimer's Disease, the method comprising the steps of obtaining a sample of cerebrospinal fluid and determining the level of ubiquitin, the level of Aβ1-42, and the level of tau present in the sample. Based on the levels of each composition in the cerebrospinal...

20060014207 - Method of measuring lipid in specific lipoprotein: A method of measuring a lipid in a specific lipoprotein characterized by using a polycyclic polyoxyalkylene derivative at least in the step of determining the specificity of the measurement of the target lipid....

20060014213 - Methods for rapid screening of mad cow disease and other transmissible spongiform encephalopathies: Methods for diagnosing altered neuropathology in an animal are disclosed, wherein said methods comprise imaging brain, spinal cord, or other neural tissue of the animal, analyzing the appearance of the tissue, and determining whether the appearance of the tissue is altered relative to corresponding unaltered tissue. Also disclosed are methods...

20060014215 - Process for detecting prpsc using an antibiotic from the family of aminoglycosides: A process of concentration by precipitation of PrPsc for detecting or diagnosing PrPsc, wherein a tissue or biological fluid stemming from or obtained from an animal or human organism is brought together with an antibiotic from the family of aminoglycosides, preferably streptomycin or one of its derivatives. The use of...

20060014212 - Proteome epitope tags and methods of use thereof in protein modification analysis: Disclosed are reagents and methods for reliably detecting the presence and measuring the amount of proteins, including proteins with various post-translational modifications (phosphorylation, glycosylation, methylation, acetylation, etc.) in a sample by the use of one or more capture agents that recognize and interact with recognition sequences uniquely characteristic of a...

20060014210 - Serial derivatization of peptides for de novo sequencing using tandem mass spectrometry: Serial derivatization by chemical reactions of analytes for mass spectrometry is disclosed. The derivatizations enhance the use of MS techniques for analyzing protein samples, particularly when the sequence of a polypeptide is determined by tandem MS/MS. Accurate mass analysis techniques are described for use in sequencing polypeptides, together with the...

20060014217 - Sphingomyelin detecting probe: An object of the present invention is to provide a protein useful as a sphingomyelin detecting probe, which specifically recognizes sphingomyelin and has low cytotoxicity. The present invention provides a protein which has an amino acid sequence having, as the amino acid sequence from the 1st to the 48th amino...

20060014214 - Urine preservation system: An improved method of preserving a molecule in a bodily fluid comprises: (1) providing a preservative solution comprising: (a) an amount of a divalent metal chelator selected from the group consisting of ethylenediaminetetraacetic acid (EDTA), (ethylenebis(oxyethylenenitrilo))tetraacetic acid (EGTA), and 1,2-bis(2-aminophenoxy)ethane-N,N,N′,N′-tetraacetic acid (BAPTA) and salts thereof in the range of from...

20060014218 - Diagnostics and therapeutics for diseases associated with neuropeptide ff receptor 1 (npff1): The invention provides a human NPFF1 which is associated with the hematological diseases, cardiovascular diseases, disorders of the peripheral and central nervous system, respiratory diseases like COPD and asthma and genito-urological disorders. The invention also provides assays for the identification of compounds useful in the treatment or prevention of hematological...

20060014219 - Method for measuring ion channel activity: The present invention discloses a human embryo kidney cell or cell line transfected with a GABA-gated chloride channel derived from an insect of the order of Lepidoptera, Diptera, Coleoptera, Homoptera, Acarina, Thysanaptera, Heteroptera, Hymenoptera or Isoptera and its use in a one-pot, high-throughput method for the measurement of the change...

20060014222 - Antibodies: The use of an ScFv Ab (ScFv Ab) capable of recognising a disease associated molecule (DAM) in the manufacture of a medicament for the prevention and/or treatment of a disease condition associated with a DAM is described. The ScFv Ab has therapeutic, diagnostic and prognostic applications....

20060014224 - Method for detecting, screening and/or monitoring a cancer in an individual: The invention relates to a method for screening and/or detecting and/or monitoring a cancer in an individual, said method comprising determining a first parameter represented by the concentration of TIMP-1 in at least one excreta, e.g. saliva, from the individual. The invention provides a method that without the need to...

20060014223 - Method for diagnosing cancer by detecting gpc3: Provided is a method for diagnosing cancer by detecting a novel cancer marker. Cancer can be diagnosed by detecting soluble glypican 3 in a test sample....

20060014225 - Vimentin directed diagnostics and therapeutics for multidrug resistant neoplastic disease: Disclosed are methods for treating or preventing a neoplastic or a multidrug resistant neoplasm in a subject using cell surface vimentin targeted therapeutic....

20060014226 - Nucleic acid and amino acid sequences of hemoglobin-response genes in candida albicans and the use of reagents derived from these sequences in the diagnosis of disseminated candida albicans infection: Three hemoglobin-response genes in the pathogenic yeast Candida albicans are disclosed. The expression of these genes is specifically induced when the organism is exposed to hemoglobin during disseminated infections. The invention relates to the nucleic acid and amino acid sequences of these hemoglobin-response genes. The invention also relates to diagnostic...

20060014228 - Bioluminescent bioreporter integrated circuit devices and methods for detecting estrogen: Bioelectronic devices for the detection of estrogen inlcude a collection of eukaryotic cells which harbor a recombinant lux gene from a high temperature microorganism wherein the gene is operably linked with a heterologous promoter gene. A detectable light-emitting lux gene product is expressed in the presence of the estrogen and...

20060014227 - Methods, compositions, and kits for the detection of bacteria in a sample: The present invention includes compositions, kits, and methods useful for the detection of bacteria. These agents and methods are primarily directed to a method of detecting the presence of bacteria in a sample, involving incubating the sample with an agent that binds to bacteria, such as, e.g., an agent specific...

20060014221 - Detection of urinary mesothelin-/megakaryocyte potentiating factor-related peptides for assessment of mesothelioma: The invention relates to methods and kits for assessing occurrence in patient urine of peptides having amino acid sequences related to those of mesothelin, megakaryocyte potentiating factor, and other peptides that have been associated with occurrence in the serum of mesothelioma patients. The methods and kits can be used to...

20060014220 - Multipurpose linker compounds and ligands and process for producing the same: A versatile linker compound has a structure represented by following general formula (1), wherein Y has a structure represented by O or NH, and X has a structure serving as a multi-branched moiety including four hydrocarbon derivative chains each of which has an aromatic amino group at an end thereof,...

20060014229 - Method and reagent for measuring cholesterol in high density lipoproteins: A method for quantitatively determining cholesterol in high-density lipoprotein in a sample, which comprises: reacting a sample with i) cholesterol esterase and cholesterol oxidase or ii) cholesterol esterase, an oxidized coenzyme and cholesterol dehydrogenase in an aqueous medium comprising i) nonionic surfactant, polyanion and albumin or ii) a combination of...

20060014230 - Beta-lactamase detecting reagent composition, detection kit and detection method: The present invention provides a reagent composition for detecting β-lactamase including as a β-lactamase detection substrate 3-[2,4-dinitrostyryl]-7-(2-thienylacetamido]-3-cephem-4-carboxylic acid, or 7-[2-(2-aminothiazol-4-yl)-2-(1-carboxy-1-methylethoxy-imino)acetamido]-3-(2,4-dinitrostyryl)-3-cephem-4-carboxylic acid, and at least one β-lactamase inhibitor selected from the group consisting of clavulanic acid, aztreonam, ethylenediaminetetraacetic acid, and cloxacillin, which composition can detect β-lactamases rapidly and easily with high...

20060014231 - Methods and compositions to promote bone homeostasis: Methods are disclosed for identifying osteogenic promoting compounds by contacting test compounds with a target gene polypeptide or fragment thereof, which target gene is identified as involved in the osteogenesis process, and measuring a compound-polypeptide osteogenesis property. Also disclosed are methods of promoting osteogenesis by contacting progenitor cells with an...

20060014232 - Immobilization method: Various biomolecules can be firmly immobilized to a substrate by a method which comprises contacting a solution containing biomolecules provided with at least one tag with an immobilization substrate which has (i) binding sites for the biomolecule tag or tags, and (ii) activated reactive groups which are capable of forming...

20060014234 - Method for the enrichment and characterization of phosphorylated peptides or proteins: A method for the separation and assay of phosphorylated peptides or proteins from complex mixtures of proteins and peptides is described. The method uses a diazo moiety linked by an organic group to a substrate. Kits for performing the assay are also described. The method and kits are particularly useful...

20060014233 - Methods for measuring adamts13 activity and protein on platelets and in plasma: Provided are assays to detect ADAMTS13 activity using peptide substrates. These assays can be used for diagnostic applications and to evaluate treatment of thrombotic thrombocytopenic purpura (TTP). Also provided is a novel form of ADAMTS13 found on platelets....

20060014235 - Quantifying exposure to stress: The present invention provides an in vitro method for quantifying exposure to psychological stress which relies on measuring the retained ability of neutrophils, preferably neutrophils in a whole blood sample, to exhibit challenge-induced superoxide anion production. Using such methodology, coping capacity of individuals for particular psychological stressors may be assessed....

20060014236 - System for detection of biological agents: The illustrative embodiment of the present invention is a system and a method for the detection and limited identification of biological agents. The system is small, light weight, requires little power to operate and uses few consumables. The system can be configured for use in either stationary or mobile applications....

20060014237 - Sensor comprising multi-wavelength leds: The illustrative embodiment of the present invention is a system and a method for the detection and identification of biological agents. The system incorporates elements that enable it to obtain an air sample, extract particulates from the air sample onto a stationary-phase collection media, expose the particulates to electromagnetic radiation,...

20060014238 - Method and system for automated detection of immunohistochemical (ihc) patterns: A method and system for automated detection of immunohistochemical (IHC) patterns. The method and system is able to automatically differentiate an epithelial cell part from a non-epithelial cell part of a digital image of a tissue sample to which an IHC compound has been applied. The method and system help...

20060014239 - Method and device for the biotechnological production of valuable products: Disclosed are a method and a device for the biotechnological production of valuable products, in which a medium is fed to a bioreactor and is subjected to a fermentation process, the valuable product is gathered as a filtered permeate and/or concentrated retentate via a cross-flow filtration system that is mounted...

20060014240 - Extraction and purification method of active constituents from stem of lonicera japonica thunb., its usage for anti-inflammatory and analgesic drug: Disclosed is a method for extracting and purifying active constituents from honeysuckle (Lonicera japonica Thunb.) and its use. More particularly, this invention relates to an extraction and purification method of active constituents including sweroside from for honeysuckle stem (stem of honeysuckle where leaves are removed) by removing tannins, hardly soluble...

20060014251 - Alternatively spliced isoform of calcium channel, voltage dependent, alpha-ii subunit (cacna1i): The present invention features nucleic acids and polypeptides encoding a novel splice variant isoform of calcium channel, voltage dependent, alpha-1I subunit (CACNA1I). The polynucleotide sequence of CACNA1Isv1 is provided by SEQ ID NO 3. The amino acid sequence of CACNA1Isv1 is provided by SEQ ID NO 4. The present invention...

20060014246 - Canine cold- and menthol-sensitive receptor 1: The present invention provides nucleic acid and polypeptide sequences describing a novel canine cold- and menthol-sensitive receptor, herein named as canine CMR1 (cCMR1). The isolated nucleic acid or polypeptide molecule of the invention can be used in detection assays and screening assays....

20060014250 - Descendants of bacteria devoid of n terminal formylation useful for the production of proteins and peptides: Eubacterial cells which produce non-formylated proteins, polypeptides and/or peptides and methods for making such non-formylated products. The eubacterial cells may be produced by inactivating or deleting the def (deformylase) and/or fmt (Met-tRNA transformylase) gene(s) from the def-fmt operon of the corresponding wild-type strain....

20060014241 - Extended glucagon-like peptide-1 analogs: The invention encompasses GLP-1 peptides with modifications at various positions coupled with an extended C-terminus that provides increased stability....

20060014252 - Flk-1 binding protein: A protein designated flk-1bp binds the vascular endothelial cell surface receptor flk-1. Isolated DNA encoding flk-1bp is provided, along with expression vectors and transformed host cells useful in producing flk-1bp polypeptides. Antibodies that are immunoreactive with flk-1bp are generated using the polypeptides disclosed herein....

20060014245 - Fusion proteins containing recombinant cytotoxic rnases: Recombinant immunotoxins containing a cytotoxic RNAse fused to an antibody or antibody fragment may be produced in mammalian cell culture. Surprisingly, immunotoxins containing a cytotoxic RNAse fused to the N-terminus of one antibody variable domain can be prepared and retain the ability to specifically bind antigen. The immunotoxins may be...

20060014243 - Human g-protein chemokine receptor hsatu68: Human G-protein chemokine receptor polypeptides and DNA (RNA) encoding such polypeptides and a procedure for producing such polypeptides by recombinant techniques is disclosed. Also disclosed are methods for utilizing such polypeptides for identifying antagonists and agonists to such polypeptides and methods of using the agonists and antagonists therapeutically to treat...

20060014249 - Human g-protein coupled receptor: A human G-protein coupled receptor polypeptide and DNA (RNA) encoding such polypeptide and a procedure for producing such polypeptide by recombinant techniques is disclosed. Also disclosed are methods for utilizing such polypeptide for identifying antagonists and agonists to such polypeptide. Antagonists and agonists may be used therapeutically to inhibit or...

20060014247 - Method and dna constructs for increasing the production level of carbohydrate degrading enzymes in filamentous fungi: The invention is related to a method and DNA constructs for obtaining in a filamentous fungus host a higher production level of a carbohydrate degrading (CD) enzyme, having in its original state a catalytic module (CAT) and a carbohydrate binding module (CBM) separated by a linker region. The DNA construct...

20060014242 - Methods of modifying feeding behavior, compounds useful in such methods, and dna encoding a hypothalamic atypical neuropeptide y/peptide yy receptor (y5): In addition, this invention provides an isolated nucleic acid molecule encoding a Y5 receptor, an isolated Y5 receptor protein, vectors comprising an isolated nucleic acid molecule encoding a Y5 receptor, cells comprising such vectors, antibodies directed to the Y5 receptor, nucleic acid probes useful for detecting nucleic acid encoding Y5...

20060014244 - Novel nucleic acid sequences encoding adenylate kinase, phospholipid scramblase-like, dna fragmentation factor-like, phosphatidylserine synthase-like, and atpase-like molecules and uses therefor: The invention provides isolated nucleic acids molecules that encode novel polypeptides. The invention also provides antisense nucleic acid molecules, recombinant expression vectors containing the nucleic acid molecules of the invention, host cells into which the expression vectors have been introduced, and nonhuman transgenic animals in which a sequence of the...

20060014248 - Tnf super family members with altered immunogenicity: The present invention relates to non-naturally occurring variant Tumor Necrosis Factor Super Family member proteins with reduced immunogenicity. More specifically, the present invention relates to variant BAFF, RANKL, TRAIL, CD40L and APRIL proteins with reduced immunogenicity....

20060014253 - Lamprey gnrh-iii polypeptides and methods of making thereof: Lamprey GnRH-III polypeptides for the four species of fish lamprey are disclosed. Also disclosed is a procedure for producing such polypeptides by recombinant techniques. Also disclosed are methods for utilizing such polypeptides for sterilizing fish....

20060014254 - Albumin fusion proteins: The present invention encompasses albumin fusion proteins. Nucleic acid molecules encoding the albumin fusion proteins of the invention are also encompassed by the invention, as are vectors containing these nucleic acids, host cells transformed with these nucleic acids vectors, and methods of making the albumin fusion proteins of the invention...

20060014255 - Methods for lyophilizing competent cells: This invention relates to a method for producing cells which are competent for transformation and which may be stably stored for extended periods of time at various temperatures. The method involves growing cells in a growth conducive medium, rendering said cells competent, and lyophilizing said competent cells. The invention further...

20060014257 - Rsf1010 derivative mob' plasmid containing no antibiotic resistance gene, bacterium comprising the plasmid and method for producing useful metabolites: A Mob− plasmid having a RSF1010 replicon, comprising a gene coding for Rep protein and said plasmid has been modified to inactivate gene related to mobilization ability. The present invention also describes a bacterium having an ability to produce useful metabolites, comprising the plasmid and said bacterium lack active thymidylate...

20060014256 - Sodium chondroitin sulfate, chondroitin-sulfate-containing material and processes for producing the same: After a solution obtained by extracting protein from cartilages of fishes which are enzyme treated is filtered, alcohol is added to a filtrate to deposit sodium chondroitin sulfate. The sodium chondroitin sulfate manufactured thus can be used for a raw material of medicines, medicine derivatives, medicine additives, cosmetics, and food...

20060014258 - Dna coding for a protein which imparts l-homoserine resistance to escherichia coli bacterium, and a method for producing l-amino acids: A bacterium which has an ability to produce an amino acid and in which a novel rhtB gene encodes a protein having an activity of making a bacterium having the protein L-homoserine-resistant is enhanced, is cultivated in a culture medium to produce and cause accumulation of the amino acid in...

20060014259 - Process for the preparation of l-amino acids with amplification of the zwf gene: The invention relates to a process for the preparation of L-amino acids. The process involves fermenting an L-amino acid producing coryneform bacteria in a culture medium, concentrating L-amino acid in the culture medium or in the cells of the bacteria, and isolating the L-amino acid produced. The bacteria has an...

20060014260 - Lower cellulase requirements for biomass cellulose hydrolysis and fermentation: A semi-continuous simultaneous saccharification and fermentation (SSF) process for the bioconversion of cellulose into ethanol and other organic chemicals is disclosed. The process provides for substantially higher substrate conversion at a given enzyme loading (or alternatively a lower enzyme loading used to achieve the same conversion) in a reactor operated...

20060014261 - Fermentative process for making inorganic nanoparticles: A method for producing mixed metal oxide compounds includes the steps of: providing a supply of a metal reducing bacteria; providing a culture medium suitable for growth of the bacteria; providing a first mixed metal oxide phase comprising at least a first and a second metal, at least one of...

20060014262 - Beta1,3-galactosyltransferase and dna encoding the same: The present invention provides a protein having δ1,3-galactosyltransferase activity, a DNA encoding the protein, a transformant comprising the DNA, a process for producing the protein using the transformant, and a process for producing a galactose-containing complex carbohydrate using the transformant....

20060014263 - Extraction and purification of phosphodiesterase: A process for purifying a phosphodiesterase 1 (PDE-1) from a cell including heating an extract of a cell formed from a solution including at least on divalent cation, to increase the specific activity of PDE-1 in the extract....

20060014264 - Cre/lox system with lox sites having an extended spacer region: The invention provides a novel Cre/lox system with lox sites having an extended spacer region. In particular, the invention provides Cre mutant polypeptides that can catalyze site-specific recombination at lox sites typically having from one to three additional base pairs in the spacer region. The Cre/lox system can be utilized...

20060014265 - Mutant alpha-amylases: Novel variant α-amylase enzymes are disclosed in which the residues corresponding to R179 and G180 in Bacillus stearothermophilus (SEQ ID NO.2) are deleted. The disclosed variant α-amylase enzymes show altered or improved stability and/or activity profiles....

20060014266 - Purification of arp2/3 complex and compositions containing purified arp2/3 complex: Methods for rapidly purifying Arp2/3 complex are provided. Compositions containing purified Arp2/3 complex that is characterized by having equal stoichiometry of the subunits are also disclosed....

20060014268 - Method for producing highly unsaturated fatty acids and lipid containing same: A method for producing highly unsaturated fatty acids comprising culturing a microorganism, belonging to the genus Mortierella and having resistance to a carbon source, in a medium having a carbon source concentration of at least 4% by weight, and collecting highly unsaturated fatty acids from the cultured products. Culturing the...

20060014267 - Novel method for growing cordyceps sinensis on a substrate and novel method for hybridizing different strains of cordyceps sinensis: Strains of Cordyceps sinensis, are grown in a substrate at 20 to 22 ° C. at sea level atmospheric pressure for 28 to 30 days in diffuse light, and thereafter in an atmosphere containing approximately 50% of oxygen of the sea level atmosphere, at approximately 3 ° C. with exclusion...

20060014269 - Device and method for providing a hybridization chamber and for influencing air bubbles in the same: A device (1), appropriate process units (18) and/or systems (38) for providing a gap-shaped hybridization space (2) to hybridize nucleic acid samples, proteins or tissue sections over a slide (3). Said items of device (1) are able to move in relation to the slide (3) and comprise an annular sealing...

20060014271 - Fabrication of a completely polymeric microfluidic reactor for chemical synthesis: An inexpensive apparatus and method of fabricating completely polymeric (e.g., SU-8, PMMA, and PEEK) microfluidic reactors suitable for the synthesis of chemicals, particularly nanoparticles (e.g., mono, bi, tri, alloy, core-shell, polymeric, and metal-polymer nano-particles), is disclosed. A high precision process uses polymeric microfluidic patterning techniques and a new microfluidic sealing...

20060014270 - System, device and method for detection of several individual analystes in a solution, and a disposible flow cell for use therein: A system, device and method for detection of several individual analytes in a test solution aliquot (83) with an array of individually operated piezoelectric crystal microbalances are described. The system comprises a connecting station (100) that receives a plurality of individually specific piezoelectric crystal microbalance flow-trough cells (10), each containing...

20060014272 - Reaction vessel and reaction product extracting apparatus: An object is to provide a reaction vessel in which, after a reaction has been conducted in the state with a cover member mounted on a reaction vessel main body, the reaction product contained in the reaction solution within the reaction vessel can be acquired without removing the cover member...

20060014273 - Cell-cultivation microchamber: A microchamber comprising a glass substrate (101) which is transparent to a specific wavelength, an absorbent region (102) which absorbs the specific wavelength, and a melting substance region (103) which does not absorb the specific wavelength, is solid at room temperature and melts when heated, which regions are layered on...

20060014274 - Fiber cassette and modularly designed cassette system: A fiber cassette has a housing that is delimited by two congruent base surfaces and at least one circumferential surface and has an interior having at least one cavity. At least one layer of fibers is arranged in the interior of the housing essentially parallel to at least one center...

20060014275 - Cell culture carrier and jig for cell culture: The present invention provides a cell culture carrier and a method for cell culture, which are capable of growing multi-layered cells or stably growing cells with a high density. To solve the above described disadvantage, the cell culture carrier of the present invention comprises cup-stacked type carbon nano tubes produced...

20060014276 - Chimeric adenoviruses: The present invention provides methods and vector systems for the generation of chimeric recombinant adenoviruses. These hybrid adenoviruses contain a genome that is derived from different adenovirus serotypes. In particular, novel hybrid adenoviruses are disclosed with improved properties for gene therapy purposes. These properties include: a decreased sensitivity towards neutralizing...

20060014277 - Novel human kielin-like proteins and polynucleotides encoding the same: Novel human polynucleotide and polypeptide sequences are disclosed that can be used in therapeutic, diagnostic, and pharmacogenomic applications....

20060014279 - Feeder independent extended culture of embryonic stem cells: Previous methods for culturing human embryonic stem cells have required either fibroblast feeder cells or a medium which has been exposed to fibroblast feeder cells in order to maintain the stem cells in an undifferentiated state. It has now been found that if an antagonist of bone morphogenic protein is...

20060014278 - Method for programmed differentiation of stem cells: Provided is a method for programming the differentiation of stem cells and for the identification of the signals responsible for cell lineage establishment of differentiated cells....

20060014280 - Compositions and methods for enrichment of neural stem cells using ceramide analogs: The present invention provides compositions and methods for human neural cell production. More particularly, the present invention provides cellular differentiation methods employing amphiphilic lipid compounds, preferably ceramide analogs of the β-hydroxyalkylamine type and optionally employing an essentially serum free MEDII conditioned medium for the generation of human neural cells from...

20060014281 - Neural stem cells: A homogenous, symmetrically dividing population of adherent neural stem cells is obtained from ES cells or foetal or adult brain isolates, using an activator of a signalling pathway downstream of a receptor of the EGF receptor family, optionally in combination with an activator of a signalling pathway downstream of an...

20060014282 - Cell and tissue engineering utilizing lif: Leukemia Inhibitory Factor (“LIF”), an LIF analogue, an LIF mimetic and a product capable of stimulating the expression of endogenous LIF, and mixtures thereof are useful, for (i) promoting the multiplication, in vitro, of a population of human skin stem cells and/or epidermal progenitors while maintaining them in an undifferentiated...

20060014283 - Inorganic bone graft materials using animal bone and method for preparing thereof: The present invention relates to inorganic bone graft materials and a method for preparing the bone graft materials. Particularly, the present invention relates to a method for preparing bone graft materials comprises the following steps: collecting cancellous bones from an animal; removing lipids and organic materials from the cancellous bones...

20060014284 - Biomatrix and method for producting the same: The invention relates to a biomatrix as well as to a method of manufacturing same. In order to provide a biologically and mechanically satisfying replacement for diseased cartilage for example, a biomatrix is proposed in which the matrix is condensed or solidified. A corresponding method is moreover proposed....

20060014285 - Methods and compositions for inhibiting biofilms: The present invention discloses compounds, compositions, and methods of using such compounds and compositions to inhibit, reduce, prevent, and remove biofilms. The invention further relates to methods of inhibiting biofilms on various substrates, such as medical devices....

20060014287 - Isolation and characterization of muscle regenerating cells: Populations enriched for myogenic progenitors are obtained by selection on the basis of expression of specific cell surface markers. The muscle progenitor cells are characterized as being CD45− and CD34+, and may further be characterized as lacking expression of Mac-1 (CD11b) and positive for expression of CXCR4 (CD184) and β1-integrin...

20060014286 - Ligand-controlled regulation of endogenous gene expression: Disclosed herein are compositions comprising an engineered zinc finger protein and a nuclear hormone receptor transcriptional control domain, polynucleotides encoding one or more components of the compositions, methods of making the compositions and methods of using these compositions, for example to modulate gene expression and/or to generate transgenic plants or...

20060014288 - Nerve regeneration: The present application describes a method of regenerating nerve, which steps include generating a recombinant viral or plasmid vector comprising a DNA sequence encoding a member of a transforming growth factor superfamily of proteins operatively linked to a promoter; transfecting in vitro a population of cultured cells with the recombinant...

20060014289 - Methods and compositions for enhancing delivery of double-stranded rna or a double-stranded hybrid nucleic acid to regulate gene expression in mammalian cells: Cholesterol moieties are linked to specific ends of double-stranded RNA, preferably a small, interfering (si)RNA or to a dsHybrid. The dsHybrid has one strand comprised of DNA and one strand comprised of RNA. Preferably the sense strand is the DNA strand and the antisense strand is the RNA strand of...

20060014290 - Inhibition of biofilm formation: The present invention relates to compositions and methods for reducing or inhibiting biofilm comprising modulating expression of a cysB gene in a cell. The invention also provides methods for modulating the expression of a cysB, cysD, cysI, cysJ, cysK, and ybiK. The invention further provides methods for identifying gene(s) involved...

20060014291 - L-rhamnose-inducible expression systems: The present invention relates to methods for expressing nucleic acid sequences in prokaryotic host cells, where at least one DNA construct which is capable of episomal replication in said host cells and which comprises a nucleic acid sequence to be expressed under the transcriptional control of an L-rhamnose-inducible promoter, where...

20060008790 - Methods and apparatuses for the automated production, collection, handling, and imaging of large numbers of serial tissue sections: An automatic taping lathe-microtome that produces a continuous ribbon of tissue by lathing an extremely thin strip off the surface of a cylindrical block containing a multitude of embedded tissue samples. Mechanisms are included for sandwiching this fragile ribbon of tissue between a pair of support tapes. Viewing holes are...

20060008791 - Dehydrating agent and method for dehydrating moist article using the agent and dehydrated article obtained by the method: The present invention has the object to dehydrate hydrous matters without denaturing or deteriorating them by using a dehydrating agent comprising an anhydrous cyclotetrasaccharide, and provides a dehydrating agent comprising the cyclotetrasaccharide; a method for dehydrating hydrous matters through a step of incorporating, contacting or coexisting the cyclotetrasaccharide into, with,...

20060008792 - Process for improvement of virus-safe biological fluids: The present invention relates to a process of disinfecting biological materials. In particular, a novel process is provided for removing detergent and/or solvent added to biological materials for the inactivation of viral contaminants. Safe, efficient, and economical methods for removing virucidal agents such as solvent-detergent from virus-inactivated pooled plasma by...

20060008794 - Hox code functions as a growth synchronizing moving reference frame: The determination of position during metazoan growth appears complex, in part, because we observe that growth does not occur at a constant rate, as temperatures fluctuate, so do the growth rates. One positioning method, a moving reference frame (MRF), is used in engineering, and navigation, to assign positions when velocity...

20060008795 - Method and an apparatus for determining well or fully differentiated mammal cells: The invention relates to a method for the in-vitro determination, especially typification, of well or fully differentiated mammal cells comprising the steps of: a) irradiating the cells with infrared light and recording the absorption spectrum obtained; b) carrying out a Fourier transformation of the absorption spectrum from (a) to obtain...

20060008796 - Methods for screening cellular proliferation using isotope labels: The present invention relates to methods for measuring the proliferation and destruction rates of cells by measuring deoxyribonucleic acid (DNA) synthesis. In particular, the methods utilize non-radioactive stable isotope labels to endogenously label DNA synthesized through the de novo nucleotide synthesis pathway in a cell. The amount of label incorporated...

20060008793 - Protein chips detecting system which can simultaneously detect multi target: The present invention provides a protein chips detecting system which can simultaneously detect multi target, and the method for manufacturing the system. The invention increases the stability and sensitivity of the system by improving the present protein chips system and the manufacture....

20060008797 - Methods of suppressing microglial activation: Methods of suppressing the activation of microglial cells in the Central Nervous System (CNS), methods of ameliorating or treating the neurological effects of cerebral ischemia or cerebral inflammation, and methods of combating specific diseases that affect the CNS by administering a compound that binds to microglial receptors and prevents or...

20060008798 - Rabbit monoclonal antibodies to hepatitis b surface antigens and methods of using the same: Reagents, methods and immunodiagnostic test kits for the accurate detection of hepatitis B virus (HBV) infection are disclosed. The methods and kits employ novel rabbit monoclonal antibodies directed against HBV surface antigens (HBsAg) with mutations in the “a” determinant region of HBsAg....

20060008841 - Aptamer capable of specifically adsorbing to verotoxin-1 and method for obtaining the aptamer: The present invention provides a method for obtaining an aptamer capable of specifically adsorbing to Verotoxin-1 produced by Enterohemorrhagic Escherichia coli by an in vitro selection method utilizing affinity chromatography, particularly, a method including use of an affinity column immobilizing thereon a peptide having an amino acid sequence of Verotoxin-1,...

20060008821 - Biomaterial inspection chip: Within an inside of a bead chip forming a reactor flow pass therein, rinsing liquid flow passes are also provided in one body. For the purpose of promoting reaction upon a bead array, a turbulence generator or an flow separation stop guide is provided within the reactor flow pass, in...

20060008818 - Carbon electrode surface for attachment of dna and protein molecules: Methods for conducting an assay to detect nucleic acid hybridization are disclosed. In particular, the fabrication of a carbon electrode suited to nucleic acid hybridization detection is described. In some preferred embodiments, a micro array of carbon electrodes is constructed using photolithography. The final step in the photolithography process involves...

20060008820 - Cell cycle genes in plants: This invention relates to an isolated nucleic acid fragment encoding a cyclin dependent kinase inhibitor (CDKI). The invention also relates to the construction of a chimeric gene encoding all or a substantial portion of the CDKI, in sense or antisense orientation, wherein expression of the chimeric gene results in production...

20060008836 - Composite arrays: A polynucleotide array, and methods of making and using such arrays. The array may include a first set of multiple features each of which has first polynucleotide molecules of at least 400 nucleotides in length, and a second set of features each of which has second polynucleotide molecules of no...

20060008837 - Compositions and methods for detecting estrogenic agents in a sample: The luxA, B, C, D, and E genes from Photorhabdus luminescens have been introduced into Saccharomyces cerevisiae bioluminescent yeast cells....

20060008808 - Compositions and methods for enhancing an immune response: The invention provides compositions and methods of enhancing an immune response in a subject. The compositions comprise eosinophil derived-neurotoxin (EDN) and/or human pancreatic ribonuclease (hPR), which can be used as adjuvants to elicit an enhanced immune response in a subject....

20060008813 - Compositions and methods of synthesis and use of novel nucleic acid structures: The present invention is directed to a method to produce 8-amino-2′deoxyadenosine by treating 8-azido-2′deoxyadenosine with an aqueous solution of methylamine or dimethylamine....

20060008815 - Compositions, methods, and systems for inferring canine breeds for genetic traits and verifying parentage of canine animals: Methods and systems are provided for managing companion animal subjects in order to maximize their individual health and potential performance and to maximize profits obtained in breeding and marketing the companion animal subjects. The methods and systems draw an inference of a phenotype for a genetic trait of a companion...

20060008823 - Dna profiling and snp detection utilizing microarrays: The present invention provides methods for rapidly identifying and distinguishing between different DNA sequences utilizing short tandem repeat (STR) analysis and DNA microarrays. Specifically, these methods facilitate the deduction of a target molecule's identity, length, and number of STRs. In an embodiment, a labeled STR target sequence is hybridized to...

20060008828 - Genotype specific detection of chlamydophila psittaci: The present invention describes novel methods for the specific detection and identification of Chlamydophila psittaci genotypes. According to one embodiment the method makes use of quantitative PCR with internal probes and optionally competitor probes which increase specificity. The invention also describes a strain of Cp. psittaci with a novel genotype...

20060008811 - Genotyping assay to predict gamma glutamyl hydrolase (ggh) activty government interest: Single nucleotide polymorphisms (SNPs) in the gene encoding gamma glutamyl hydrolase (GGH) associated with reduced GGH activity are disclosed. The primary SNP is a change from a cytosine to a thymine at a position corresponding to nucleotide 511 of Genbank sequence accession no. NM 003878. Methods and kits for detecting...

20060008803 - Identification of tissue/cell specific marker genes and use thereof: A cartilage array comprises a plurality of different polynucleotide probe spots stably associated with a solid surface of a carrier, whereby each of said spots is made of a unique polynucleotide that corresponds to one specific cartilage marker gene. Said specific cartilage marker genes preferably are at least in part...

20060008805 - Information processing system using base sequence-related information: This invention constructs a highly safe system for processing information for providing semantic information and/or information associated with the semantic information useful for each individual organism through effective utilization of differences in nucleotide sequence-related information among individual organisms. This system comprises steps of: (a) obtaining positional information representing a position...

20060008834 - Life sciences business systems and methods: Improved life sciences business systems and methods are disclosed. One or more genomes are scanned for single nucleotide polymorphisms. The polymorphisms are assigned to haplotype blocks, and representative SNPs from the haplotype blocks are used in association studies for pharmaceutical and diagnostic developments....

20060008804 - Marker genes: Methods are disclosed for fast and accurate readout of kidney toxicity before it occurs and before it is demonstrated by histopathology examination. Ultimately, this approach shall allow earlier compound selection. The twelve genes identified, namely Calbindin-D28k, KIM-1, OPN, EGF, Clusterin, VEGF, OAT-K1, Aldolase A, Aldolase B, Podocin, Alpha-2u and C4,...

20060008814 - Method for determining a risk of atherosclerosis or future development of atherosclerotic complications: A method for determining a risk of atherosclerosis or future development of atherosclerotic complications by measuring an amount of a soluble Fcγ receptor IIIaMφ derived from macrophage (hereafter abbreviated as sFcγRIIIaMφ) in a blood sample from patients by using an antibody specifically recognizing FcγRIIIMφ, and determining the risk on the...

20060008826 - Method for determining alleles: The present invention provides methods and kits for separating and identifying alleles, and thereby the haplotype, in genomic DNA samples. The method generally involves hybridizing primers specific to polymorphic sites within the alleles to the DNA sample, elongating the primers by one or more nucleic acids, separating the elongated primers...

20060008833 - Method for long, error-reduced dna synthesis: A method for synthesizing a long, error-corrected DNA construct is disclosed. In the method, error-containing subregions of a long DNA sequence are replaced by repair oligonucleodides that are short enough that the probability of any one of them containing an error is less than one. Repeated repair cycles lead to...

20060008806 - Method for producing novel dna sequence with biological activity: A method of obtaining an oligonucleotide capable of carrying out a predetermined biological function. A heterogeneous pool of oligonucleotides, x+y+z nucleotides in length, is first generated. Each oligonucleotide has a 5′ randomized sequence, x nucleotides in length, a central preselected sequence, y nucleotides in length, and a 3′ randomized sequence,...

20060008809 - Method for quantitative pcr data analysis system (qdas): This invention provides a method for the quantitation and quality assurance of QPCR data. In particular, the invention provides a method for extracting data curves from a QPCR assay, estimating the shape and characteristics of an amplification curve and producing a quality score metric. The invention also provides a robust...

20060008802 - Method for the identification of inhibitors of the binding of are-containing mrna and a hur protein: The present invention provides for a method for identifying an agent that has an inhibitory effect on the complex-formation of an ARE-containing mRNA and an HuR protein....

20060008824 - Methods and compositions for clonal amplification of nucleic acid: Described herein are methods and compositions relating to amplifying nucleic acid. In certain embodiments, the invention provides methods for labeling and amplifying a nucleic acid molecule....

20060008810 - Methods and compositions for detecting rhinoviruses: The invention provides methods and compositions for rapid, sensitive, and highly specific nucleic acid-based (e.g., DNA based) detection of human rhinovirus (HRV) in a sample. In general, the methods involve detecting a target nucleic acid having a target sequence of a conserved 5′ untranslated region of the HRV genome. The...

20060008825 - Methods and compositions for determining responsiveness to antibody therapy: Methods and compositions are provided for determining whether a subject suffering from a neoplastic condition is responsive to an antineoplastic therapy, such as antibody therapy, e.g., Rituximab. In practicing the subject methods, the subject is genotyped to determine whether the subject has a least one favorable FcγR polymorphism, e.g., the...

20060008817 - Methods and compositions for generating recombinant nucleic acid molecules: A method of generating a double stranded (ds) recombinant nucleic acid molecule covalently linked in both strands by contacting two or more ds nucleotide sequences with a topoisomerase under conditions such that both termini of at least one end of a first ds nucleotide sequence are covalently linked by the...

20060008831 - Methods and systems for predicting protein-ligand coupling specificities: The invention provides methods and systems for predicting or evaluating protein-ligand coupling specificities. A pattern recognition model can be trained by selected sequence segments of training proteins which have a specified ligand coupling specificity. Each selected sequence segment is believed to include amino acid residue(s) that may contribute to the...

20060008832 - Methods for identifying a candidate for treatment of obesity: Methods of identifying human subjects for treatment of obesity or an obesity-related condition with ciliary neurotrophic factor (CNTF) or a CNTF-related molecule, such as Axokine®, based on the presence of human leukocyte antigen (HLA) DRβ1 allele 1501 or the absence of HLA DRβ1 allele 0701....

20060008801 - Molecules associating to c-terminal domain in receptor cell: Concerning intracellular signal transduction mechanism, there has been drawn a novel hypothesis that, even in the case where phosphorylation does not occur in the intracellular C-terminal domain of a receptor, an unknown molecule associates with the Pro-C terminal domain of a G protein-coupled receptor for each chemokine and thus leukocyte...

20060008829 - Multimarker panel based on pigf for diabetes type 1 and 2: The present invention relates to a method and means for diagnosing or risk stratification of co-morbidities, particularly cardiovascular complications in diabetes patients. The invention particularly relates to a method for diagnosing whether a diabetes patient is suffering from a cardiovascular complication or is at risk of suffering from a cardiovascular...

20060008807 - Multiparameter analysis of comprehensive nucleic acids and morphological features on the same sample: A highly sensitive assay is disclosed which utilizes a method for gene specific primed amplification of mRNA libraries from rare cells and rare transcripts found in blood. The assay allows detection of rare mRNA (10 copies/cell) found in 1 to 10 cells isolated through immunomagnetic enrichment. The assay is an...

20060008819 - Novel 38594, 57312, 53659, 57250, 63760, 49938, 32146, 57259, 67118, 67067, 62092, fbh58295fl, 57255, and 57255alt molecules and uses therefor: The invention provides isolated nucleic acids molecules, designated 38594, 57312, 53659, 57250, 63760, 49938, 32146, 57259, 67118, 67067, 62092, FBH58295FL, 57255, and 57255alt nucleic acid molecules, which encode transporter molecules, including sugar transporters, organic anion transporters, amino acid transporters, and phospholipid transporters. The invention also provides antisense nucleic acid molecules,...

20060008830 - Nucleic acid end-labeling reagents: Compounds and methods are provided for covalent end-labeling of polynucleotides. Incorporation of a nucleic acid affinity group improves the efficiency of reaction of aldehyde reactive groups with the nucleic acid leading to more efficient labeling....

20060008835 - Pna monomer and precursor: wherein E is nitrogen or C—R′; J is sulfur or oxygen; R′, R1, R2, R3, R4 is independently H, halogen, alkyl, nitro, nitrile, alkoxy, halogenated alkyl, halogenated alkoxy, phenyl or halogenated phenyl, R5 is H or protected or unprotected side chain of natural or unnatural α-amino acid; and B is...

20060008816 - Promoter, promoter control elements, and combinations, and uses thereof: The present invention is directed to promoter sequences and promoter control elements, polynucleotide constructs comprising the promoters and control elements, and methods of identifying the promoters, control elements, or fragments thereof. The invention further relates to the use of the present promoters or promoter control elements to modulate transcript levels....

20060008838 - Prostate cancer diagnosis and outcome prediction by expression analysis: Methods identifying prostate cancer, methods for prognosing and diagnosing prostate cancer, methods for identifying a compound that modulates prostate cancer development, methods for determining the efficacy of a prostate cancer therapy, and oligonucleotide microarrays containing probes for genes involved in prostate cancer development are described....

20060008799 - Rapid haplotyping by single molecule detection: The present invention includes a method for rapid haplotyping a DNA or RNA segment. Two or more target sites on a segment of DNA or RNA are labeled with separate distinguishable luminescent hybridization probes, where the targets are selected genetic markers. A dilute solution is formed containing the labeled DNA...

20060008822 - Single-stranded and double-stranded oligonucleotides comprising a 2-arylpropyl moiety: One aspect of the present invention relates to a double-stranded oligonucleotide comprising at least one aralkyl ligand. In certain embodiments, an aralkyl ligand is bound to only one of the two oligonucleotide strands comprising the double-stranded oligonucleotide. In certain embodiments, an aralkyl ligand is bound to both of the oligonucleotide...

20060008840 - Substrate preparation process: The present invention provides novel processes for the large scale preparation of arrays of polymer sequences wherein each array includes a plurality of different, positionally distinct polymer sequences having known monomer sequences. The methods of the invention combine high throughput process steps with high resolution photolithographic techniques in the manufacture...

20060008812 - System having device for preventing air bubbles in a hybridization chamber and corresponding method: The present invention relates to a system (1) having hybridization chambers (5) for hybridizing nucleic acid samples, proteins, or tissue sections immobilized on slides (27), each hybridization chamber (5) being defined as an essentially gap-shaped chamber, which is essentially fillable with a liquid, between one of these slides (27) and...

20060008800 - Unc-13 in the modulation of neurotransmission and secretion events: The present invention relates to a method for identifying and/or obtaining a molecule which is capable of modifying secretion processes comprising the steps of a) contacting an unc-13 molecule or (a) part(s) or (a) fragment(s) thereof with a candidate molecule; b) measuring and/or detecting a response; and c) comparing said...

20060008839 - Utr tag assay for gene function discovery: The methods of the invention provide a means for rapid analysis of gene function in a variety of systems. The invention allows screening of large libraries of nucleotide sequences for involvement in physiological pathways of interest. The methods of the invention also provide an efficient means of identifying and isolating...

20060008846 - Antagonism of tgf-beta superfamily receptor signaling: Members of the TGF-β superfamily control many physiologic and pathophysiologic processes in multiple tissues and signal via type II and type I receptor serine kinases. Type II activin receptors are promiscuous and known to bind 12 TGF-β ligands including activins, myostatin, BMPs and nodal. Methods are described for the screening...

20060008844 - C-met kinase binding proteins: Polypeptides comprising monomer domains that bind to c-Met, or portions thereof, are provided....

20060008850 - Combinatorial synthesis of peg oligomer libraries: A simple chain-extending approach was established for the scale-up of the monoprotected monodisperse PEG diol materials. Reactions of THP-(OCH2CH2)n—OMs (n=4, 8, 12) with a large excess of commercially available H—(OCH2CH2)n—OH (n=1-4) under basic conditions led to THP-(OCH2CH2)n—OH (n=5-15). Similarly, Me-(OCH2CH2)n—OH (n=4-11, 13) were prepared from Me-(OCH2CH2)n—OMs (n=3, 7, 11). For...

20060008843 - Determination of protein function: For purposes of determining the function of a protein, an automated system captures images of cells, each cell located in a predetermined well. After a given cell is exposed to a protein of interest, the system measures the responses of the cell over time, evaluating a variety of cellular parameters....

20060008852 - Marine actinomycete taxon for drug and fermentation product discovery: The invention is the discovery of an actinomycete genus, given the name Salinospora gen. nov., that displays an obligate requirement of seawater (Na+) for growth and unique 16S rRNA signature nucleotides. The invention is also the use of the genus for the production and discovery of active biomolecules such as...

20060008847 - Mechanical cartridge with test strip fluid control features for use in a fluid analyte meter: A structure for controlling fluid flow from a sample receiving pad into a lateral flow assay test strip, including: a lateral flow assay test strip; a sample pad abutting the lateral flow assay test strip; a pinch wall positioned to direct fluid flow from the sample pad to the lateral...

20060008842 - Method of measuring neprilysin activity: The present invention provides a method of measuring the activity of neprilysin, etc. More specifically, the present invention provides a method of measuring the activity of neprilysin in nerve cells; a method of screening a protein, a peptide or a compound enhancing the activity or expression of neprilysin nerve cells...

20060008849 - Methods for measuring chloride channel conductivity: The invention provides non-radioactive methods to assay for functional chloride channels. The methods colorimetrically detect the amount of iodide conducted by a chloride channel. They can be easily adapted for high throughput assays or screenings....

20060008851 - Methods for rapid and quantitative proteome analysis: The invention provides methods for identifying a polypeptide. The method can include determining two or more characteristics associated with the polypeptide, or a fragment thereof, one of the characteristics being mass of a fragment of the polypeptide, the fragment mass being determined by mass spectrometry; comparing the characteristics associated with...

20060008845 - Polypeptide compositions formed using a coiled-coil template and methods of use: The invention relates to the use of constrained coiled-coil polypeptides to mimic α-helical structural elements of native proteins. These constrained peptidyl mimetics are used to generate and/or identify ligands which selectively bind the α-helical segment contained in the native protein....

20060008848 - Stabilized compounds having secondary structure motifs: The present invention provides novel stabilized crosslinked compounds having secondary structure motifs, libraries of these novel compounds, and methods for the synthesis of these compounds libraries thereof. The synthesis of these novel stabilized compounds involves (1) synthesizing a peptide from a selected number of natural or non-natural amino acids, wherein...

20060008854 - Method of determining time of death: A method of determining time of death is disclosed. Cardiac troponin I (cTnI) is the most specific marker for the determination of myocardial infarction (MI). Cardiac troponin I has a distinctive temporal degradation profile after death, which is key to its use as a time of death marker in forensic...

20060008853 - Monoclonal antibodies directed against the microtubule-associated protein tau and method of detecting the same: A monoclonal antibody which forms an immunological complex with a phosphorylated epitope of an antigen belonging to human abnormally phosphorylated tau protein. The tau protein can be obtained from a brain homogenate, itslf isolated from the cerebral cortex of a patient having Alzheimer's disease. Further, a method of detecting the...

20060008855 - Promiscuous g-protein compositions and their use: Disclosed are compositions and methods for their use, such as in identifying G-protein coupled receptors and ligands and compounds that modulate signal transduction. The compositions and methods employ promicuous G-proteins. Activation of the promiscous G-protein can be detected in a variety of assays, including assays in which activation is indicated...

20060008857 - Cap-binding protein: A novel polypeptide which is useful in screening of an agent for improving insulin resistance and an agent for improving glucose metabolism, a polynucleotide encoding the polypeptide, an expression vector comprising the polynucleotide, and a cell transformed with the expression vector are disclosed. The polypeptide is a protein expressed in...

20060008858 - Osteoblast factor(s) that regulates human prostate cancer migration to and invasion of bone: Human osteogenic cells secrete biological activities that stimulate cancer cells to migrate to and/or invade tissues. Conditioned medium (CM) produced by human pre-osteoblasts and osteoblasts induced migration and tissue invasion of human prostate cancer cells. Thus, conditioned media and/or proteins isolated therefrom may be used to identify metastasis-inducing factors. Also...

20060008859 - Transfusion registry network providing real-time interaction between users and providers of genetically characterized blood products: Disclosed is a registry system, including member institutions, in which transfusion donors and recipients are registered following genotyping, which would typically take place in a member institution, or a member institution would have access to the genotyping information, if performed outside. The registry database can be accessed and searched by...

20060008856 - Mass tags for quantitative analysis: The present invention relates generally to novel protein modification reagents for fractionation and quantitative (differential) profiling of proteins in a complex mixture. The reagents react with amino acids or other protein components or structures and function as mass tags. The present invention provides methods of making the protein modification reagents...

20060008860 - Microbial atp extraction and detection system: The present invention is directed to compositions and methods for single-step extraction and detection of ATP levels from microbial cells. The disclosed compositions are formulated to efficiently elicit bioluminescent detection of ATP among a broad variety of different microorganisms using a common single-step reagent composition. Additional luminescence-based methods are provided...

20060008861 - Incorporation of anaerobic bacteria in feed formulation: Anaerobic bacteria are incorporated in a feed such that they provide a benefit to the consuming animal. Anaerobic bacteria can be viable and colonize the gut to provide this benefit by displacing harmful bacteria, secreting a particular agent (e.g., enzyme, antibiotic or bioactive compound), binding or sequestering harmful organisms or...

20060008862 - Real time methylumbelliferone-based assay: A method is provided for determining the activity of an enzyme which releases methylumbelliferone (MU) from an MU-containing substrate wherein the enzyme has a pH optimum below the pKa of MU comprising: contacting a sample suspected of containing the enzyme with the MU-containing substrate at a pH suitable for activity...

20060008863 - Electrochemical sensing using an enzyme electrode: Electrodes for use in electrochemical assays to determine whether a candidate drug is metabolised by an oxidative drug-metabolising enzyme (DME) are described. In a first type of electrode the DME is immobilised at the surface of the electrode. In a second type of electrode, a surface of the electrode is...

20060008864 - Methods to measure compound specificity: A system is presented that uses a microassay which measures the activity of a tubulin ligand on mammalian brain (standard) and cancer cell (pathogen) tubulin, and by calculation of the Tubulin Ligand Index a comparison can be made as to the potency of the compound towards cancer cell tubulin and...

20060008866 - Apparatus and method for detecting human fecal contamination on hands and other objects using an illumination imaging device: According to the present invention there is disclosed a system and a method for detecting the presence of human fecal contamination on objects, such as a protein source, a worker's hands, work surfaces, tools or utensils. In one embodiment, there is included a supporting structure which supports a diffuse light...

20060008867 - Microorganism culture medium: A microorganism culture medium comprising a derivative A′ of a chromogen A, and a chromogen B, where the chromogen A is a chromogen being oxidized to a colorant, and the chromogen B is a chromogen being reduced to a colorant. The derivative A′ acts as a substrate of an enzyme...

20060008865 - Screening apparatus for water treatment with membranes: A static screen used upstream of a membrane assembly within a water treatment system has a screening surface with a number of openings distributed over its area. Liquid flows through the screening surface to reach the membrane assembly. Various shapes of screening surfaces are described including undulating panels and geometric...

20060008868 - Production method of biochemical coal: A production method of biochemical coal, comprises: a) providing at least a first carbon-containing substance and a second carbon-containing substance; b) pulverizing said first carbon-containing substance and said second carbon-containing substance; c) mixing a first proportion amount of said first carbon-containing substance, a second proportion amount of said second carbon-containing...

20060008869 - Process for production of biopterin compound: The present invention provides a transformed cell which is transformed by at least one gene of enzymes participating in biosynthesis of tetrahydrobiopterin and a process for the production of a biopterin compound using the same. In accordance with the present invention, the biopterin compound can be produced in large quantities...

20060008871 - Extract of e. coli cells having mutation in ribosomal protein s12, and method for producing protein in cell-free system using the extract: It is intended to provide an extract, kit and a process for synthesizing a protein in a cell-free system. The extract is characterized by being prepared from mutant Escherichia coli cells having a mutation in the ribosomal protein S12 gene. In a preferred embodiment, the mutation is such a mutation...

20060008870 - Method for universal enzymatic production of bioactive peptides: The invention provides methods for making peptides from a polypeptide containing at least one copy of the peptide using clostripain to excise the peptide from the polypeptide. The methods enable the use of a single, highly efficient enzymatic cleavage to produce any desired peptide sequence....

20060008873 - Cloning and sequencing of allergens of dermatophagoides (house dust mite): Isolated DNA encoding allergens of Dermatophagoides (house dust mites) particularly of the species Dermatophagoides farinae and Dermatophagolides pteronyssinus, which are protein allergens or peptides which include at least one epitope of the protein allergen. In particular, DNA encoding two major D. farinae allergens, Der f I and Der f II...

20060008877 - Expression systems with sec-system secretion: Improved methods and prokaryotic expression systems for producing recombinant proteins using Sec-system secretion signal peptides are described using Pseudomonas fluorescens Sec secretion systems. Specific novel Sec-system secretion signal peptides are described, as are fusion proteins and coding sequences for improved secretion of recombinant proteins and peptides....

20060008876 - Me-5, me-2, and epp2: human protein antigens reactive with autoantibodies present in the serum of women suffering from endometriosis: Three human endometrial antigens, ME-5, ME-2 and EPP2, and combination thereof have been discovered that are the targets of antibodies present in the serum of women suffering from endometriosis. These protein products and the human antibodies reactive with them are used as tools for diagnostic assays that monitor patients with...

20060008872 - Method of improving efficacy of biological response-modifying proteins and the exemplary muteins: Disclosed is a protein variant which substitutes valine for phenylalanine residue in a binding domain having a biological response-modifying function by binding to a receptor, ligand or substrate. Also, the present invention discloses a DNA encoding the protein variant, a recombinant expression vector to which the DNA is operably linked,...

20060008878 - Monomeric red fluorescent proteins: Disclosed are sequences encoding monomeric variants of DsRed fluorescent proteins and methods of use....

20060008880 - Mouse trace amine associated receptors and polypeptides: The present invention provides a fingerprint sequence which is specific and selective for trace amine associated receptors (TAAR) forming a subfamily of G protein coupled receptors. The invention also provides the novel mouse polypeptides identified as members of this family, nucleic acids encoding said polypeptides, and vectors and host cells...

20060008875 - Novel tfiih subunit: The present invention pertains to a nucleic acid sequence encoding a human TFIIH 8 kDa subunit and related sequences. The nucleic acids may be used in methods for producing a TFIIH subunit, as well as in methods for diagnosing or treating transcription and NER deficiencies, in particular in some forms...

20060008874 - Plant transcriptional regulators of abiotic stress: The invention relates to plant transcription factor polypeptides, polynucleotides that encode them, homologs from a variety of plant species, variants of naturally-occurring sequences, and methods of using the polynucleotides and polypeptides to produce transgenic plants having advantageous properties, including improved cold and other osmotic stress tolerance, as compared to wild-type...

20060008879 - Polypeptides having alpha-glucosidase activity and polynucleotides encoding same: The present invention relates to isolated polypeptides having alpha-glucosidase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides....

20060008881 - Mammalian diabetes-mediating proteins: Provided are mammalian secreted and non-secreted diabetes mediating proteins, including protective and deleterious diabetes-mediating proteins, as well as polynucleotides encoding same, drug screening methods for identifying a test compound capable of altering the expression of a diabetes-mediating protein, and methods of preventing or ameliorating diabetes by administering a compound capable...

20060008882 - Antibodies to chemokine alpha-5: The present invention relates to a novel CKα-5 protein which is a member of the alpha chemokine family. In particular, isolated nucleic acid molecules are provided encoding the human CKα-5 protein. CKα-5 polypeptides are also provided, as are vectors, host cells and recombinant methods for producing the same. Antibodies and...

20060008883 - Methods of generating variant proteins with increased host string content and compositions thereof: The present invention relates to novel methods for generating variant proteins with increased host string content, and proteins that are engineered using these methods....

20060008884 - Hybrid adenovirus/adeno-associated virus vectors and methods of use thereof: The invention provides recombinant vectors including adenovirus/adeno-associated virus (Ad/AAV) vectors and mini-adenovirus (mAd) vectors. Further, the invention provides cells containing these vectors, and methods for making and using the vectors and cells. The compositions and methods of the invention are useful in transferring nucleotide sequences of interest into a cell,...

20060008885 - Method for glucose production using endoglucanase core protein for improved recovery and reuse of enzyme: The present invention pertains to a method of converting cellulose to glucose by treating a pretreated lignocellulosic substrate with an enzyme mixture comprising cellulase enzyme and endoglucanase core proteins, wherein the endoglucanase core proteins are present in the enzyme mixture at an amount relative to all endoglucanases from about 35...

20060008886 - Portable shooting house: A hunting house, comprising: an elongated tongue 150, a removable axle 350, a framework 700, and a camouflaged exterior 650 fitted over said framework 700....

20060008887 - Production of carboxylic acid and carbonic acid derivatives using a thermostable esterase: The present invention relates to processes for the production of acyl compounds using an esterase having thermostable properties, and to products of such processes....

20060008888 - Polypeptide: We describe a food additive comprising a PS4 variant polypeptide, in which the PS4 variant polypeptide is derivable from a parent polypeptide having non-maltogenic exoamylase activity, in which the PS4 variant polypeptide comprises an amino acid substitution at position 223 with reference to the position numbering of a Pseudomonas saccharophilia...

20060008889 - Dsp-10 dual-specificity phosphatase: Compositions and methods are provided for the treatment of conditions associated with cell proliferation, cell differentiation and cell survival. In particular, the dual-specificity phosphatase DSP-10, and polypeptide variants thereof that stimulate dephosphorylation of DSP-10 substrates, are provided. The polypeptides may be used, for example, to identify antibodies and other agents...

20060008890 - Polypeptide: We describe a food additive comprising a PS4 variant polypeptide, in which the PS4 variant polypeptide is derivable from a parent polypeptide having non-maltogenic exoamylase activity, in which the PS4 variant polypeptide comprises an amino acid substitution at position 121 with reference to the position numbering of a Pseudomonas saccharophilia...

20060008891 - Cathepsin g-related peptides as modulators of formylpeptide receptors (fpr): The present invention relates, e.g., to a method for identifying a peptide of CaG, or an active variant thereof, that modulates a CaG-FPR response (e.g., chemotaxis), comprising screening a candidate CaG peptide or active variant thereof for its ability to modulate a CaG-FPR induced response. Methods for identifying peptide mimetics...

20060008892 - Methods of and pharmaceutical compositions for improving implantation of embryos: Methods of improving embryo implantation are disclosed and comprise contacting a receptive uterus and/or an embryo with an effective amount of heparanase and implanting the embryo in the receptive uterus....

20060008893 - Nanoengineered biophotonic hybrid device: An improved method for the design and development of high performance hybrid devices having biological and nonbiological components. A figure of merit is developed for the biological component or components. The component is subjected to various environmental variables as it or its biological source organism is grown. The biological component...

20060008894 - Yeast model for amyloidogenic protein toxicity: The present invention relates to an engineered yeast strain, comprising a transgene encoding one or more amyloidogenic proteins or mutant(s) thereof, characterised in that it lacks one or more functional genes responsive to oxidative stress. The invention further relates to the use of this yeast strain for pharmaceutical screening, for...

20060008896 - Electronic analyte assaying device: The invention is an electronically processed single-step test device for detecting the presence of a preselected analyte in a fluid. The device includes a hollow rectangular outer casing, disposed within co-joined upper and lower sections of the casing are assay material, an electronic processing system, and a LCD display. The...

20060008897 - Lens assembly for biological testing: The invention relates to an optical detection system for a thermal cycling device including at least one light source, a light detection device for detecting light received from a plurality of biological samples, and a lens having first and second surfaces formed on the lens, the second surface substantially opposed...

20060008895 - Microarray chip for detection of immunoglobulin: Disclosed is a microarray chip for allergy-related immunoglobulin detection, especially for quantitative detection of total IgE and allergen-specific immunoglobulins (such as specific IgE, specific IgG, and specific IgM), which comprises a solid substrate, a reactive layer fabricated on the solid substrate, and at least one allergen or substance capable of...

20060008898 - Industrial composting system: An industrial composting system is provided. Organic matter to be composted is received in a first hopper at a receiving station and structuring elements are poured in a second hopper. The contents of both hoppers are discharged together on a transfer conveyor in a controlled manner. The resulting composting mix...

20060008899 - Method and device for carrying out biochemical reactions with a high throughput: Device, method and kit for carrying out biochemical reactions, in particular for cell-free polypeptide biosynthesis composed of an external housing which encloses an inner housing with incorporated wells and a supply chamber, wherein the wells of the inner housing each contain a producing system during the biochemical reaction, the supply...

20060008900 - Pigment epithelium-derived factor: characterization, genomic organization and sequence of the pedf gene: Nucleic acids encoding the neurotrophic protein known as pigment epithelium-derived factor (PEDF), a truncated version of PEDF referred to as rPEDF, and equivalent proteins, vectors comprising such nucleic acids, host cells into which such vectors have been introduced, recombinant methods for producing PEDF, rPEDF, and equivalent proteins, the rPEDF protein...

20060008901 - Secreted and transmembrane polypeptides and nucleic acids encoding the same: The present invention is directed to novel polypeptides and to nucleic acid molecules encoding those polypeptides. Also provided herein are vectors and host cells comprising those nucleic acid sequences, chimeric polypeptide molecules comprising the polypeptides of the present invention fused to heterologous polypeptide sequences, antibodies which bind to the polypeptides...

20060008902 - Method of forming mesenchymal stem cells from embryonic stem cells: This invention relates to methods of producing a substantially homogenous population of mesenchymal stem cells derived from embryonic stem cells. Also, disclosed is a homogenous population of mesenchymal stem cells capable of further differentiating into a variety of specific cell types, characterized by various morphological factors and cell-specific markers. The...

20060008903 - Method and apparatus for collecting cells for macromolecular analysis: A method for rapidly collecting cells from a surface, such as a surface bearing fingerprints, for subsequent macromolecular analysis involves dispensing a predetermined amount of an aqueous solution onto the surface, and subjecting the aqueous solution to ultrasound waves to promote a detachment of the cells from the surface. Extraction...

20060008904 - Cell culture method and apparatus thereof: By rotating a cell-culture container while controlling the temperature and the gaseous atmosphere, cells are cultured under the application of hydrostatic pressure due to centrifugal force, and according to this cell culture, cells are efficiently activated and cultured while inhibiting dedifferentiation....

20060008905 - Collagen/glycosaminoglycan compositions for use as terminally sterilizable matrices: Compositions of cross-linked collagen and a glycosaminoglycan are provided which retain characteristics rendering them useful as tissue engineering matrices or scaffolds following terminal sterilization. Also provided are methods for producing these compositions and terminally sterilized matrices or scaffolds from these compositions as well as methods of using these matrices or...

20060008906 - Electrofusionof cells and apparatus therefore: The present invention relates to a method and apparatus for fusing first and second cells. In particular, the method includes positioning the first and second cells between two electrodes (35) in a fluid filled container (40) using a pipette system (33), with the first and second cells being held separated...

20060008907 - Control of gene expression via light activated rna interference: A method for controlling the spacing, timing and degree of gene expression that includes selecting a target mRNA, obtaining siRNA corresponding to the target mRNA, modifying the siRNA with a photo-labile group to inhibit RNA interference, introducing the modified siRNA into a cell, and selectively irradiating the cell with light...

20060008908 - Method and composition for longevity assurance: This present invention provides compositions and methods for Longevity Assurance and treatment of disorders associated with age-related diseases. The compositions and methods further address age-related or incorrect or abnormal regulation of cellular homeostasis by controlling mechanisms of systemic, intracellular and extracellular ionic physiology through the administration of alkaline salts. The...

20060008910 - Lipid encapsulated interfering rna: The present invention provides lipid-based formulations for delivering, e.g., introducing, nucleic acid-lipid particles comprising an interference RNA molecule to a cell, and assays for optimizing the delivery efficiency of such lipid-based formulations....

20060008909 - Liposomal formulations comprising dihydrosphingomyelin and methods of use thereof: The present invention includes novel liposomes comprising dihydrosphingomyelin. The invention also includes compositions comprising these liposomes and a therapeutic agent, in addition to methods and kits for delivering a therapeutic agent or treating a disease, e.g., a cancer, using these compositions....

20060008911 - Methods and compositions for regulating gene expression in plant cells: Novel chimeric plant promoter sequences are provided, together with plant gene expression cassettes comprising such sequences. Novel transcription factors are provided, together with nucleic acid sequences encoding such transcription factors and plant gene expression cassettes comprising such nucleic acid sequences. Methods for using the chimeric plant promoter sequences and novel...

20060003308 - Electrocompetent cells prepackaged for electroporation: Electrocompetent cells are prepackaged in a sterilized cuvette, and the cell contents frozen, such that electroporation can be performed simply by first thawing the cuvette contents, then electrically connecting the cuvette to a power source suitable for electroporation. The cuvette contains, in addition to the cells, a suspending medium, and...

20060003309 - Method of frozen donor egg banking: A method is provided for a frozen egg bank comprising a) retrieving egg cells from an egg donor; b) cryopreserving said eggs in a cryopreservation solution; c) quarantining said eggs; and d) testing the egg donor for an infectious agent following a quarantine period of time. Optionally the method may...

20060003312 - Circulating stem cells and uses related thereto: The disclosure provides, inter alia, methods for enhancing the contribution of circulating stem cells to a target tissue. Such methods may be useful for treating a variety of disorders. In a preferred embodiments, circulating stem cell contribution is enhanced by causing damage to the target tissue, or by administering an...

20060003310 - Device for performing cell assays: A device for performing cell assays has at least one assay site. The site comprises a pair of stimulating electrodes and a cell confinement cavity for confining at least one cell in an extracellular fluid medium. The cell confinement cavity and the stimulating electrodes are arranged so that, in use,...

20060003311 - In vitro screening of cellular events using 3d cell culture systems: The present invention relates to in vitro cell culture conditions wherein transfected cells containing promoter-reporter constructs are cultivated in a 3D tissue-like environment. The referred 3D culture conditions may eventually implement appropriate biomaterial or scaffolds....

20060003313 - Methods for identifying factors for differentiating definitive endoderm: Disclosed herein are methods of identifying one or more differentiation factors that are useful for differentiating cells in a cell population comprising definitive endoderm cells into cells which are capable of forming tissues and/or organs that are derived from the gut tube....

20060003319 - Compositions and methods for determining resistance to inhibitors of virus entry using recombinant virus assays: The invention provides a method for determining whether a human immunodeficiency virus is resistance to a viral entry inhibitor. The methods are particularly useful for determining resistance to inhibitors that act by a non-competitive mechanism. In certain aspects, the methods comprise determining whether an HIV population is resistant to an...

20060003317 - Drug discovery method: The present invention relates to drug discovery methods, particularly antiviral drug discovery methods....

20060003320 - Exploring fluorophore microenvironments: Methods, apparatus, and system, implementing and using techniques for detecting a presence of one or more target analytes in particular regions of interest of one or more samples. One or more samples including objects and one or more target analytes are provided. Some of the target analytes are labeled with...

20060003321 - Expression monitoring for human cytomegalovirus (hcmv) infection: Certain human genes have been found to be induced or repressed in host cells infected with HCMV. A large set of such genes has been identified. These have diagnostic use in determining the extent of tissue damage caused by the infection as well as in determining the stage of disease...

20060003316 - Immunogenic compositions derived from poxviruses and methods of using same: Immunogenic compostions composed of poxvirus immunogens and related methods are disclosed. Specifically, immunogenic compostions useful in eliciting immune responses in animals are disclosed. In one embodiment the immunogenic compostions include viral antigens derived from vaccinia and/or variola that elicit cross-reactive immune responses. The immunogens can be made synthetically, by using...

20060003318 - Influenza sensor: A sensor for the detection of tetrameric multivalent neuraminidase within a sample is disclosed, where a positive detection indicates the presence of a target virus within the sample. Also disclosed is a trifunctional composition of matter including a trifunctional linker moiety with groups bonded thereto including (a) an alkyl chain...

20060003315 - Membrane-anchored beta2 microglobulin covalently linked to mhc class 1 peptide epitopes: The invention provides a polynucleotide comprising a sequence encoding a polypeptide comprising a beta2-microglobulin molecule linked through its carboxyl terminal to a polypeptide stretch that allows the anchorage of the beta2-microglobulin molecule to the cell membrane, and through its amino terminal to at least one antigenic peptide comprising a MHC...

20060003314 - Methods for peptide and protein display in nucleic acid arrays: Virus displayed peptides or proteins can be integrated and presented in a nucleic acid array format. The present invention makes it possible to generate naked nucleic acidvirion protein display complexes in which the individual nucleic acid template is freely and covalently linked to the very same virion proteins it coded...

20060003373 - Automated process for isolating and amplifying a target nucleic acid sequence: An automated analyzer for performing multiple diagnostic assays simultaneously includes multiple stations, or modules, in which discrete aspects of the assay are performed on fluid samples contained in reaction receptacles. The analyzer includes stations for automatically preparing a specimen sample, incubating the sample at prescribed temperatures for prescribed periods, preforming...

20060003322 - Bioinformatically detectable group of novel regulatory genes and uses thereof: The present invention relates to a first group of novel genes, here identified as “genomic address messenger” or “GAM” genes, and a second group of novel operon-like genes, here identified as “genomic record” or “GR” genes. GAM genes selectively inhibit translation of known ‘target’ genes, many of which are known...

20060003341 - Cloned genes encoding reverse transcriptase lacking rnase h activity: The invention relates to a gene which encodes reverse transcriptase having DNA, polymerase activity and substantially no RNase H activity. The invention also relates to vectors containing the gene and hosts transformed with the vectors of the invention. The invention, also relates to a method of producing reverse transcriptase having...

20060003356 - Compositions and methods for the diagnosis and treatment of kidney disease: Polymorphisms associated with the CD2AP protein are disclosed. Compositions and methods for the diagnosis and treatment of kidney disease are also disclosed....

20060003337 - Detection of small rnas: Methods for detecting an RNA such as a small RNA comprising up to about 40 nucleotides are disclosed. The methods comprise forming a ligation mixture comprising a sample suspected of comprising the small RNA, a ligase and a target probe set. The target probe set comprises a first target probe...

20060003362 - Ent1 transporters expressed in cancer cells: ENT1 is consistently expressed at high levels in cancer cells. Disclosed herein are assays for determining whether a test material/molecule is a substrate for, and/or is transported by, the ENT1 transporter, and therefore a candidate substrate for transport into cancer cells. The assays are useful in screening for cytotoxic agents...

20060003342 - Fetal rna in amniotic fluid to determine gene expression in the developing fetus: The present invention provides improved methods of prenatal diagnosis, monitoring, screening and/or testing. The invention is based, at least in part, on the discovery that amniotic fluid is a rich source of cell-free fetal RNA. Methods of isolation and analysis of fetal RNA are described, that can lead to information...

20060003364 - Glut1 transporters expressed in cancer cells: GLUT1 is consistently expressed at high levels in cancer cells. Disclosed herein are assays for determining whether a test material/molecule is a substrate for, and/or is transported by, the GLUT1 transporter, and therefore a candidate substrate for transport into cancer cells. The assays are useful in screening for cytotoxic agents...

20060003363 - Glut3 transporters expressed in cancer cells: GLUT3 is consistently expressed at high levels in cancer cells. Disclosed herein are assays for determining whether a test material/molecule is a substrate for, and/or is transported by, the GLUT3 transporter, and therefore a candidate substrate for transport into cancer cells. The assays are useful in screening for cytotoxic agents...

20060003380 - Human tumor necrosis factor receptor tr-17: The present invention relates to a novel protein, TR17, which is a member of the tumor necrosis factor (TNF) receptor superfamily. In particular, isolated nucleic acid molecules are provided encoding the human TR17. TR17 polypeptides are also provided as anti TR17 antibodies and vectors, host cells and recombinant methods for...

20060003372 - Integration of direct binding label-free biosensors with mass spectrometry for functional and structural characterization of molecules: The invention provides methods for the detection, quantification, identification and structural analysis of one or more molecules. Mass spectrometry (MS) is not a universal detector as all molecules do not ionize equally well leading to poor signal to quantity information. MS can be optimized to identify the specific mass of...

20060003331 - Isolation of antisense oligonucleotides: The present invention relates to a method of isolating fully thioated single stranded antisense oligonucleotides from a biological solution, which method comprises the steps of contacting the biological solution with an immobilised metal ion adsorption chromatography (IMAC) resin to adsorb the antisense oligonucleotides to said resin and subsequently contacting the...

20060003352 - Mass tag pcr for mutliplex diagnostics: This invention provides a mass tag-based method for simultaneously detecting in a sample the presence of one or more of a plurality of different target nucleic acids. This invention also provides related kits...

20060003328 - Method and system for enhancing data quality: The quality (e.g., resolution) of image data, video data, and sound data representing a scene or signal is enhanced by a quality enhancement function trained on high quality and low quality representations of a portion of the same scene or signal. The enhancement function is thus optimized to the most...

20060003360 - Method for analyzing variation of nucleic acid and method for analyzing gene expression: A method for determining the type of a base at a target site in a target nucleic acid, comprising a step of reacting a target nucleic acid sample with a probe nucleic acid which contains a selection sequence complementary to the 3′ side-nucleotide sequence adjacent to the target site and...

20060003332 - Method for multiplex pna-fish: This invention is related to multiplex PNA-FISH assays....

20060003324 - Method for producing gene libraries: The presented invention refers to a method for the creation of gene libraries wherein a defined number of adjacent nucleotides is exchanged and gene libraries are produced which code for protein variants having more manifold amino acid exchanges and a more homogenous distribution of mutations than can be obtained using...

20060003346 - Method for treating hepatitis: Hepatitis can be treated by administering to a patient in need thereof an effective amount of a compound that neutralizes the effects of secreted TNF-alpha....

20060003325 - Method of detecting base mutation: The present invention provides a nucleotide mutation detection method wherein primers having a nucleotide sequence complementary to part of the nucleic acids to be detected and also having a nucleotide sequence, which is complementary to the nucleotide sequence just upstream from a nucleotide site corresponding to the mutation site of...

20060003374 - Method of making pna oligomers: wherein E is nitrogen or C—R′; J is sulfur or oxygen; R′, R1, R2, R3, R4 is independently H, halogen, alkyl, nitro, nitrile, alkoxy, halogenated alkyl, halogenated alkoxy, phenyl or halogenated phenyl, R5 is H or protected or unprotected side chain of natural or unnatural α-amino acid; and B is...

20060003354 - Methods and compositions for the diagnosis of cornelia de lange syndrome: Compositions and methods for the diagnosis of Cornelia de Lange Syndrome are disclosed....

20060003350 - Methods and compositions to detect bacteria using multiplex pcr: A novel multiplex polymerase chain reaction assay for detecting a bacteria (e.g. Helicobacter pylori) in a specimen uses multiple oligonucleotide primer pairs based on the sequences of multiple loci of the bacteria. In one application, up to five loci in the genomic DNA sequences of Helicobacter pylori were amplified. Two...

20060003351 - Methods and kits for identifying target nucleotides in mixed populations: Ligation-based methods and kits are disclosed for identifying at least two target nucleotides in a mixed population sample, that is a sample that contains or potentially contains target nucleic acid sequences from more than one source. Typically, two ligation reaction compositions are formed, ligation products generated, and the ligation products...

20060003371 - Methods and reagents for improved selection of biological molecules: Coated Ferromagnetic Density Particles or Density Particles with binding agents bound thereto capable of binding biological molecules and methods of use and apparatus for means are disclosed. Coated particles coupled to specific binding agents can be used for molecular biology and proteomic applications in research and diagnostics....

20060003335 - Methods for diagnosing acute megakaryoblastic leukemia: The present invention is directed to methods and compositions for use in the diagnosis of acute megakaryoblastic leukemia. More particularly, it is shown that mutations in exon 2 of GATA-1 correlated with a predisposition to acute megakaryoblastic leukemia. Methods and compositions for exploiting this finding are described....

20060003359 - Methods of screening for cell proliferation or neoplastic disorders: The invention relates to methods and compositions for identifying subjects having, or predisposed to having, a neoplastic or cell proliferation or neoplastic disorder. The methods are applicable to any type of tissue sample and can be conducted on otherwise normal tissue....

20060003344 - Methods related to a single nucleotide polymorphism of the g protein coupled receptor, gpr40: This invention relates to genotyping methods, methods of treatment, diagnostic tests and kits and methods of characterizing an agent, related to a single nucleotide polymorphism of the GPR40 gene....

20060003326 - Modified l-nucleic acid: The present invention relates to a modified L-nucleic acid, comprising a L-nucleic acid part and a non-L-nucleic acid part, whereby the L-nucleic acid part is conjugated to the non-L-nucleic acid part and the conjugation of the L-nucleic acid part with the non-L-nucleic acid past leads to a slowed elimination out...

20060003340 - Multi-allelic molecular detection of sars-associated coronavirus: The subject invention relates to a multiple-allelic RT-real-time polymerase chain reaction (PCR) assay for coronaviruses including the SARS virus. Multiple target sequences within the SARS-CoV, S, E, M and N genes are identified. The use of the four different targets enhances the likelihood that the fundamental genetic drift of the...

20060003366 - Multiplexing assays for analyte detection: High density multiplexing assays for proteins, nucleic acids, and other molecules are described based upon using mass spectrometry detection. A solid-state support assay format is utilized, whereby, the bead carries both a coding molecule to track the sample or analyte and the molecule(s) necessary to perform an affinity-based assay. After...

20060003368 - Nicotinamide n-methyltransferase as a marker for colorectal cancer: The present invention relates to the diagnosis of colorectal cancer. It discloses the use of the protein nicotinamide N-methyltransferase (NNMT) in the diagnosis of colorectal cancer. It relates to a method for diagnosis of colorectal cancer from a liquid sample, derived from an individual by measuring NNMT in said sample....

20060003376 - Non-destructive quality control method for microarray substrate coatings via labeled doping: A non-destructive method of determining the uniformity of a coating on a substrate comprises coating the substrate with a homogenous coating mixture comprising a labeled compound and a chemically functional compound to which other chemical moieties can be bound, and detecting the presence and locations of the labeled compound on...

20060003375 - Novel polypeptide - human retinoic acid-responsive protein 53.57 and a polynucleotide encoding the same: A novel polypeptide-human retinoic acid-responsive protein 53.57 and a polynucleotide encoding the same, as well as a method of producing the polypeptide by DNA recombinant technique. The present invention also discloses methods of using the polypeptide in treatment of various disease, such as arhythmia, bronchial asthma, peptic ulcer, diabetes mellitus,...

20060003348 - Omi pdz modulators: The invention provides modulators of Omi PDZ-ligand interaction, and methods of identifying and using these modulators....

20060003336 - One-step enzymatic and amine detection technique: A technique for detecting the presence or quantity of an enzyme (or enzyme inhibitor) and/or an amine within a test sample is provided. For example, in one embodiment, a diagnostic test kit is employed that utilizes reactive complexes that each includes a substrate joined (e.g., covalently bonded, physically adsorbed, etc.)...

20060003378 - Peptidoglycan recognition proteins: The present invention related to three novel recognition protein expressed by keratinocytes, wound-healing tissues and chondrosarcoma tissue. More specifically, isolated nucleic acid molecules are provided encoding human peptidoglycan recognition protein-related proteins, referred to herein as PGRP-K (Keratinocytes), PGRP-W (Wound-healing), and PGRP-C (Chondrosarcoma) of FIGS. 1A-B, FIGS. 2A-C, and FIG. 3,...

20060003327 - Peripheral blood cell markers useful for diagnosing multiple sclerosis and methods and kits utilizing same: Markers of multiple sclerosis and methods and kits utilizing same for diagnosing multiple sclerosis in an individual are provided....

20060003358 - Polymorphic kinase anchor proteins and nucleic acids encoding the same: Polymorphic A-kinase anchor proteins (AKAPs) and nucleic acids encoding the proteins are provided herein. Methods of detecting polymorphic AKAPs and nucleic acids encoding the AKAPs, and kits for use in the detection methods are also provided. Further provided herein are methods of identifying subjects having or at risk of developing...

20060003367 - Polynucleotides encoding a novel human kupffer cell receptor protein, bgs-18: The present invention provides novel polynucleotides encoding BGS-18 polypeptide, fragments and homologues thereof. Also provided are vectors, host cells, antibodies, and recombinant and synthetic methods for producing said polypeptides. The invention further relates to diagnostic and therapeutic methods for applying these novel BGS-18 polypeptide to the diagnosis, treatment, and/or prevention...

20060003333 - Preparation of defined highly labeled probes: A method for producing a single-stranded unitized nucleic acid probe comprising the acts of: (a) contacting an oligonucleotide primer having a 5′ recognition end having a length of between about 6 to 50 nucleotides and having a 3′ priming end having a length of between about 6 to 50 nucleotides...

20060003343 - Primer and probe design for efficient amplification and detection of hcv 3' non-translating region: The present invention provides selected oligonucleotides corresponding to portions of the 3′ non-translating region of Hepatitis C virus. The invention also includes methods of detecting and of quantitating HCV nucleic acids in a sample using these oligonucleotides....

20060003334 - Protein (poly)peptides libraries: The present invention relates to synthetic DNA sequences which encode one or more collections of homologous proteins/(poly)peptides, and methods for generating and applying libraries of these DNA sequences. In particular, the invention relates to the preparation of a library of human-derived antibody genes by the use of synthetic consensus sequences...

20060003369 - Provision of dna investigating tools: A method of evaluating primers for use in the amplification of DNA sequences, a method of producing a mixture of primers for such a purpose and a method of amplifying a plurality of DNA sequences using a mixture of primers is provided in which the evaluation involves performing one or...

20060003353 - Recombinant dna processes using a dntp mixture containing modified nucleotides: Amplification mixtures, kits, amplicons, kits and processes are provided for amplifying a nucleic acid. In particular, provided are processes which utilize an amplification mixture comprising a polymerase, a deoxynucleotidetriphosphate (dNTP) mixture which contains modified dNTPs, a first primer and a second primer. Further provided are dNTP mixtures which contain modified...

20060003345 - Rna bioassay: The present invention relates to methods for characterizing the pathological state of the kidney, methods of characterizing an agent, methods of evaluating the kidney protective and therapeutic characteristics of an agent and methods of monitoring the effect of a pharmaceutical agent on the kidney of a subject, related to measuring...

20060003355 - Secreted and transmembrane polypeptides and nucleic acids encoding the same: The present invention is directed to novel polypeptides and to nucleic acid molecules encoding those polypeptides. Also provided herein are vectors and host cells comprising those nucleic acid sequences, chimeric polypeptide molecules comprising the polypeptides of the present invention fused to heterologous polypeptide sequences, antibodies which bind to the polypeptides...

20060003370 - Secreted and transmembrane polypeptides and nucleic acids encoding the same: The present invention is directed to novel polypeptides and to nucleic acid molecules encoding those polypeptides. Also provided herein are vectors and host cells comprising those nucleic acid sequences, chimeric polypeptide molecules comprising the polypeptides of the present invention fused to heterologous polypeptide sequences, antibodies which bind to the polypeptides...

20060003347 - Single-molecule in vitro evolution: The invention relates to a method for isolating one or more genetic elements encoding a gene product having a desired activity, comprising the steps of: a) providing a population of genetic elements and expressing the genetic elements to produce their respective gene product(s), such that each molecule of gene product...

20060003379 - Smad6 and uses thereof: The invention includes nucleic acids encoding the Smad6 protein, including fragments and biologically functional variants thereof. Also included are polypeptides and fragments thereof encoded by such nucleic acids, and antibodies relating thereto. Methods and products for using such nucleic acids and polypeptides also are provided....

20060003361 - Smvt transporters expressed in cancer cells: SMVT is consistently expressed at high levels in cancer cells. Disclosed herein are assays for determining whether a test material/molecule is a substrate for, and/or is transported by, the SMVT transporter, and therefore a candidate substrate for transport into cancer cells. The assays are useful in screening for cytotoxic agents...

20060003357 - Solid phase technique for selectively isolating nucleic acids: A method of isolating target nucleic acid molecules from a solution comprising a mixture of different size nucleic acid molecules, in the presence or absence of other biomolecules, by selectively facilitating the adsorption of a particular species of nucleic acid molecule to the functional group-coated surface of magnetically responsive paramagnetic...

20060003330 - Streptococcus pneumoniae pbp2x mini-protein and uses thereof: Modified recombinant protein derived from Streptococcus pneumoniae PBP2x, referred to as mini-PBP2x, consisting of a concatenation of the fragments corresponding, respectively, to the amino acids located between positions 74 to 90, 186 to 199, 218 to 228 and 257-750, with reference to the sequence of the PBP2x protein of the...

20060003338 - System and methods for the management and treatment of vascular graft disease: Various embodiments of the present invention are directed to a system and methods for minimizing risk factors that contribute to the development of vascular graft disease and vascular graft failure. In one embodiment, a microarray-based gene expression analysis may be employed to select pre-implanted vessel candidates suitable as grafts in...

20060003377 - Therapeutic and diagnostic proteins comprising a socs box: The present invention relates generally to therapeutic and diagnostic agents. More particularly, the present invention provides therapeutic molecules capable of modulating signal transduction such as but not limited to cytokine-mediated signal transduction. The molecules of the present invention are useful, therefore, in modulating cellular responsiveness to cytokines as well as...

20060003323 - Therapeutic polypeptides, nucleic acids encoding same, and methods of use: Disclosed herein are nucleic acid sequences that encode novel polypeptides. Also disclosed are polypeptides encoded by these nucleic acid sequences, and antibodies that immunospecifically bind to the polypeptide, as well as derivatives, variants, mutants, or fragments of the novel polypeptide, polynucleotide, or antibody specific to the polypeptide. Vectors, host cells,...

20060003349 - Tm leveling compositions: Modified oligonucleotides are provided containing bases selected from unsubstituted and 3-substituted pyrazolo[3,4-d]pyrimidines and 5-substituted pyrimidines, and optionally have attached minor groove binders and reporter groups....

20060003329 - Treating cancer: Provided is a method for screening for effective agents for the treatment of cancer which method comprises selecting a putative agent that is likely to disrupt a function mediated by a critical normal gene product, which function is required for the successful division and continued cell survival of cancer cells,...

20060003339 - Two-hybrid system: Methods of detecting targets in a sample are provided. Methods of quantitating targets in a sample are provided. Methods of determining the specificity of a first molecule for a second molecule are provided. Methods of detecting inhibitors or enhancers of an interaction between a first molecule and a second molecule...

20060003365 - Use of gene expression profiling to predict survival in cancer patient: Gene expression profiling in multiple myeloma patients identifies genes that distinguish between patients with subsequent early death or long survival after treatment. Poor survival is linked to over-expression of genes such as ASPM, OPN3 and CKS1B which are located in chromosome 1q. Given the frequent amplification of 1q in many...

20060003382 - Compositions and methods for analyte detection: The invention relates to novel methods and compositions for the detection of analytes using the nuclear reorganization energy, λ, of an electron transfer process. In particular, the present invention provides probes of the outer-sphere environment of a redox center and methods of correlating changes in electrochemical properties to characterize the...

20060003384 - Detection of measurement of antibodies to antigenic proteins in biological tissues or samples: The present invention relates to methods and compositions for detecting and/or measuring serum antibodies to antigenic proteins in a sample, comprising adding a labeled antigenic protein or fragment thereof to a sample derived from serum and expected to contain serum antibodies and measuring differences in at least one characteristic between...

20060003383 - Fluorogenic enzyme activity assay methods and compositions using fragmentable linkers: Substrate compound-containing micelles and various compositions, kits and methods for their preparation and use are provided....

20060003385 - Mass intensity profiling system and uses thereof: The present invention is directed to computer automated methods and systems for identifying and characterizing biomolecules in a biological sample. Mass spectrometry measurements are obtained on biomolecules in a sample. These measurements are analyzed to determine the abundance of the biomolecules in the sample, and the abundance measurements are coupled...

20060003386 - Metallopeptide combinatorial libraries and applications: Method of making metallopeptide combinatorial libraries and metallopeptides are provided, for use in biological, pharmaceutical and related applications. The combinatorial libraries are made of peptides, peptidomimetics and peptide-like constructs, and include a metal ion-binding region thereof which includes at least one orthogonal sulfur-protecting group, in which the peptide, peptidomimetic or...

20060003381 - Methods for assembling protein microarrays: The invention provides methods for assembling protein arrays. In one aspect, the methods comprise use of charged or polar array surfaces in the construction of the array. The methods also comprise use of viscous solutions for the deposition of the polypeptides on the array surface in the construction of the...

20060003387 - Cell display libraries: Eukaryotic cell display libraries for use in panning processes comprising expressed biomolecules for specific and selective binding and enrichment to solid material surfaces including, for example, metal, magnetic, and semiconducting surfaces. Display can be regulated. Peptide and protein display on yeast cells are preferred. Solid materials can be fabricated in...

20060003388 - Sensitive intracellular calcium assay: A sensitive intracellular calcium assay is disclosed comprising conveniently a reagent comprised of a dye precursor capable of entering cells and being hydrolyzed to a dye, whereby the dye complexes with calcium in said cells and provides a luminescent signal, an antibody specific for the dye and conjugated with a...

20060003391 - Reagents and methods for use in cancer diagnosis, classification and therapy: Methods and reagents for classifying tumors and for identifying new tumor classes and subclasses. Methods for correlating tumor class or subclass with therapeutic regimen or outcome, for identifying appropriate (new or known) therapies for particular classes or subclasses, and for predicting outcomes based on class or subclass. New therapeutic agents...

20060003392 - Methods of diagnosing inflammatory bowel disease: The present invention provides methods for diagnosing the presence or severity of inflammatory bowel disease (IBD) in an individual and methods for stratifying IBD by determining the level of one or more IBD markers in a sample from the individual and calculating an index value using an algorithm based upon...

20060003393 - Pathogenicity determinants which can be used as targets for developing means for preventing and controlling bacterial infections and/or systemic dissemination: The invention relates to a method for identifying and selecting a gene required for the proliferation in vivo of a pathogenic microorganism, comprising:—using a strain of the pathogenic microorganism,—generating mutants for inactivation in the genes encoding these factors,—determining the virulence of these mutants on an expiremental model of infection, and...

20060003389 - Detection of post-translationally modified peptides with liquid crystals: A method for differentiating between a post-translationally modified peptide and a peptide contained in a sample, comprising: (a) contacting the sample with a peptide attachment surface to create a peptidized surface, wherein the sample includes at least one functional group; (b) contacting the peptidized surface with a recognition reagent that...

20060003390 - Method for immobilizing conjugates in diagnostic tests: The invention concerns a method for the detection of an analyte in a sample using analyte-specific conjugates which have at least one heterologous group for an analyte-independent binding to a control zone. The present invention additionally provides new conjugates and reagent kits....

20060003394 - Enzymatic detection techniques: A diagnostic test kit for detecting the presence or quantity of an enzyme or enzyme inhibitor is provided. The diagnostic kit utilizes reactive complexes to facilitate the detection of the enzyme or enzyme inhibitor. The reactive complexes include a substrate joined (e.g., covalently bonded, physically adsorbed, etc.) to a reporter...

20060003395 - Measurement method using biosensor: It is an object of the present invention to provide a method for simultaneously carrying out the detection or measurement of a substance interacting with a physiologically active substance immobilized on a biosensor and the analysis of the effects of the above substance on the biological activity of the physiologically...

20060003396 - Screening arrangement for screening immunoassay tests and agglutination tests: Immunoassay tests or agglutination tests run on samples of bodily fluid to detect the presence of particular compounds such as drugs in the body may be screened in the screening device. A test membrane is inserted into the screening device and illuminated. The reflected image is detected and the digitised...

20060003398 - Subcutaneous glucose electrode: A small diameter flexible electrode designed for subcutaneous in vivo amperometric monitoring of glucose is described. The electrode is designed to allow “one-point” in vivo calibration, i.e., to have zero output current at zero glucose concentration, even in the presence of other electroreactive species of serum or blood. The electrode...

20060003397 - Test element: The invention concerns test elements and methods for the optical detection of an analyte in a sample. The test element includes a support having at least one transparent portion, and a one-layer film positioned on the support. The film has a thickness when dry of less than about 10 μm....

20060003399 - High throughput actin polymerization assay: Methods for assaying for actin polymerization are described, as are the use of the assays to screen for modulators of actin polymerization. The screening methods can be utilized to identify active agents that interact with actin, the polymerization state of actin and other proteins or cellular components whose function is...

20060003400 - Methods and compositions for characterizing a redox reagent system enzyme: Methods and compositions for characterizing a redox reagent system enzyme are provided. In practicing the subject methods, a sample that includes a redox reagent system enzyme and a known amount of substrate is applied to an electrochemical cell that includes an enzyme-free reagent composition having a redox reagent system mediator....

20060003401 - Method for preparing a water-soluble carbon nanotube wrapped with self-assembly materials: A water-soluble carbon nanotube (CNT) wrapped with self-assembly material, and a method for preparation thereof, in which a mixture of the self-assembly material and CNT is provided, and the mixture induces a self-assembly of the self-assembly material on the CNT, thereby wrapping the CNT with the self-assembly material. Water-soluble CNT...

20060003402 - Continuous flow reaction systems with controlled addition of reactants: An apparatus for performing continuous flow chemical reactions such as oxidation, oxidative dehydrogenation and partial oxidation processes involving a reactor design characterized by controlled/optimized addition of a reactant with the objective of: (i) avoiding the explosion regime of the reactant mixture (e.g., hydrocarbon/oxidant mixture); (ii) maximizing the selectivity of the...

20060003403 - Carotenoid ketolase genes with improved ketocarotenoid yield: Protein engineered CrtO ketolases are provided having increased carotenoid ketolase activity. Methods using the present CrtO ketolases are also provided for increasing ketocarotenoid production in suitable production hosts....

20060003408 - Acid-stable alpha amylases having granular starch hydrolyzing activity and enzyme compositions: The present invention relates to an acid-stable alpha amylase (asAA) derived from a strain of Aspergillus kawachi, which has granular starch hydrolyzing (GSH) activity, the heterologous expression of the asAA having GSH activity in filamentous fungal host cells and enzyme compositions including the same which optionally include glucoamylase....

20060003416 - Means and methods for producing a protein through chromatin openers that are capable of rendering chromatin more accessible to transcription factors: Described are means and methods for providing a cell with a protein expression unit, the method comprising providing a nucleic acid sequence comprising the unit with a nucleic acid sequence encoding a binding site for a member of a chromatin modification system for rendering chromatin more accessible for transcription (opener),...

20060003421 - Method for purifying a fermentation-derived product: Process for purifying a fermentation-derived product....

20060003404 - Method for specific integration of t7 rna polymerase gene in the chromosome of corynebacterial and the resultant corynebacteria-t7 promoter based shuttle vector system: The present invention relates to method for obtaining optimum expressed proteins in a transformed gram positive bacteria by specific integration of T7 RNA polymerase gene into the chromosome of a gram positive bacteria exhibiting resistance to aminoglycosides, said method comprising:- digesting an E. coli plasmid with a restriction enzyme- digesting...

20060003405 - Method of expressing recombinant protein in cho cells: Method of expressing recombinant protein in CHO cells, by using an expression vector comprising the murine IgG 2A gene locus....

20060003407 - Methods for preparing highly active april ligand polypeptides: The present invention relates to improved methods for producing biologically active truncated APRIL ligand polypeptides and analogs thereof. The invention further relates to truncated APRIL ligand polypeptides and analogs thereof that retain a high biological activity and may be isolated in high yields, as well as the nucleotide sequences that...

20060003420 - Monomeric and dimeric fluorescent protein variants and methods for making same: The present invention relates generally to variant fluorescent proteins, and more specifically to monomeric and dimeric forms of Anthozoan fluorescent proteins. In one aspect, the present invention provides variants of fluorescent proteins, where the variants have a reduced propensity to tetramerize, and form dimeric or monomerc structures. The invention also...

20060003411 - Mutated d-aminotransferase and method for producing optically active glutamic acid derivatives using the same: A D-aminotransferase can be modified so as to efficiently produce (2R, 4R)-monatin having high sweetness intensity from 4-(indol-3-ylmethyl)-4-hydroxy-2-oxoglutaric acid by substituting an amino acid at least at one of positions (positions 100, 180 to 183,243 and 244) involved in efficiently producing the (2R, 4R)-monatin in an amino acid sequence of...

20060003413 - Novel human protein kinase, phosphatase, and protease family members and uses thereof: The invention provides isolated nucleic acids molecules, designated 53070, 15985, 26583, 21953, m32404, 14089, and 23436 nucleic acid molecules, which encode novel human protein kinase family members, serine/threonine protein kinase family members, serine/threonine phosphatase family members, prolyl oligopeptidase family members, trypsin family members, trypsin serine protease family members, and ubiquitin...

20060003409 - Novel molecules of the card-related protein family and uses thereof: Novel CARD-12 polypeptides, proteins, and nucleic acid molecules are disclosed. In addition to isolated CARD-12 proteins, the invention further provides CARD-12, fusion proteins, antigenic peptides and anti-CARD-12 antibodies. The invention also provides CARD-12 nucleic acid molecules, recombinant expression vectors containing a nucleic acid molecule of the invention, host cells into...

20060003417 - Peptides acting as both glp-1 receptor agonists and glucagon receptor antagonists and their pharmacological methods of use: The invention provides polypeptides that act both as an agonist of the GLP-1 receptor and an antagonist of the glucagon receptor. Such polypeptides are useful for treating individuals with type 2 diabetes or other metabolic disorders....

20060003418 - Peptides acting as both glp-1 receptor agonists and glucagon receptor antagonists and their pharmacological methods of use: The invention provides polypeptides that act both as an agonist of the GLP-1 receptor and an antagonist of the glucagon receptor. Such polypeptides are useful for treating individuals with type 2 diabetes or other metabolic disorders....

20060003419 - Peptides acting as both glp-1 receptor agonists and glucagon receptor antagonists and their pharmacological methods of use: The invention provides polypeptides that act both as an agonist of the GLP-1 receptor and an antagonist of the glucagon receptor. Such polypeptides are useful for treating individuals with type 2 diabetes or other metabolic disorders....

20060003406 - Process of purifying vancomycin hydrochloride: Provided is a method of purifying a vancomycin from a fermentation broth of a microorganism containing vancomycin, including passing the fermentation broth of a microorganism containing vancomycin through a strong acid cation exchange resin, a weak base anion exchange resin, alumina and a hydrophobic absorbent resin sequentially....

20060003410 - Prostaglandin reductase: An isolated polypeptide containing the sequence of SEQ ID NO: 1 or a functional equivalent of SEQ ID NO:1, in which the polypeptide reduces 15-keto prostaglandin but not leukotriene B4. Also disclosed is an antibody that binds specifically to the polypeptide and a double-stranded ribonucleic acid for inhibiting expression of...

20060003412 - Protein engineering with analogous contact environments: The invention relates to novel methods for engineering protein sequences using structural and homology information....

20060003414 - Signal peptide for producing a polypeptide: The present invention relates to a method for producing a polypeptide comprising using a signal peptide, to nucleic acid constructs comprising a first nucleotide sequence encoding the signal peptide and a second nucleotide sequence encoding a polypeptide which is foreign to the first nucleotide sequence. Furthermore, it also relates to...

20060003415 - Stable acanthamoeba protein expression systems: The present invention relates to stable expression systems for production of a protein comprising: a) a host organism selected from the group consisting of the species of Acanthamoeba; b) a DNA vector which comprises a selection marker gene which codes for a protein which, after transformation of the host organism,...

20060003422 - Method for the identification of substances mimicking mammal epitopes: A method for the preparation of specific monoclonal immunological binding molecules having binding capacity to mammal epitopes, comprising the following steps: a) isolation of structures containing said epitopes to obtain an epitope preparation; b) immunization of non-mammals with the epitope preparation to obtain an immune response; c) immortalization of the...

20060003423 - Process for the preparation of l-amino acids using strains of the enterobacteriaceae family which contain an enhanced succ or sucd gene: The invention relates to a process for the preparation of L-amino acids, in particular L-threonine, in which the following steps are carried out: a) fermentation of microorganisms of the Enterobacteriaceae family which produce the desired L-amino acid and in which at least one or more of the genes chosen from...

20060003424 - Method of constructing amino acid producing bacterial strains, and method of preparing amino acids by fermentation with the constructed amino acid producing bacterial strains: A method of producing coryneform bacteria having an improved amino acid- or nucleic acid-productivity comprises the steps of introducing a mutation in a promoter sequence of amino acid- or nucleic acid-biosynthesizing genes on a chromosome of a coryneform bacterium to make it close to a consensus sequence or introducing a...

20060003425 - Method for zymotic production of fine chemicals (meta) containing sulphur: The invention relates to methods for the fermentative production of sulfur-containing fine chemicals, in particular L-methionine, by using bacteria which express a nucleotide sequence coding for a methionine synthase (metA) gene....

20060003426 - Novel aldolase, and method for producing optically active ihog and monatin: The present invention relates to a method for producing optically active IHOG, which can in turn be used for the production of monatin. The present invention further relates to a method for producing optically active monatin, and aldolase used for these methods. As such, the present invention enables the synthesis...

20060003427 - Enzymatic synthesis of n (alpha)-acyl-l-arginine esters: The invention relates to a process for preparing a Na-acyl-L-arginine ester, derived from fatty acids and esterified dibasic amino acids, according to the following formula (I), where: X— is Br—, Cl—, or HSO4- R1: is linearalkyl chain from an saturated fatty acid, or hydroxy-acid from 8 to 14 atoms of...

20060003428 - Enzymatic resolution of an alpha-substituted carboxylic acid or an ester thereof by carica papaya lipase: Disclosed herein is a process for enzymatically resolving a mixture of R- and S-enantiomers of an α-substituted carboxylic acid or an ester or thioester thereof, in which a Carica papaya lipase is used as a biocatalyst to effect the resolution as desired....

20060003429 - Biocatalytic synthesis of quinic acid and conversion to hydroquinone: A bioengineered synthesis scheme for the production of quinic acid from a carbon source is provided. Methods of producing quinic acid from a carbon source based on the synthesis scheme as well as conversion of quinic acid to hydroquinone are also provided....

20060003430 - Lysophosphatidic acit acetyltransferases: An isolated nucleic acid fragment encoding an LPAAT isozyme is disclosed. Construction of a chimeric gene encoding all or a portion of the LPAAT isozyme, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the LPAAT isozyme in a transformed host...

20060003431 - Structure of protein kinase c theta: A three-dimensional structure of protein kinase C theta (PKCθ) can be used in methods of designing an agent that interacts with PKCθ. The agent can be an inhibitor of PKCθ activity....

20060003432 - Novel purified polypeptides from enterococcus faecalis: The present invention relates to novel drug targets for pathogenic bacteria. Accordingly, the invention provides purified protein comprising the amino acid sequence set forth in SEQ ID NO: 4. The invention also provides biochemical and biophysical characteristics of the polypeptides of the invention....

20060003433 - Xylanases, nucleic acids encoding them and methods for making and using them: The invention relates to xylanases and to polynucleotides encoding the xylanases. In addition, methods of designing new xylanases and methods of use thereof are also provided. The xylanases have increased activity and stability at increased pH and temperature....

20060003434 - Mammalian endonucleases and methods of use: Isolated mammalian Mus81-Eme endonuclease complexes comprise an Mus81 protein portion and an Eme protein portion. A method of identifying a chemical compound that modulates mammalian cellular response to DNA damage comprises contacting a chemical compound to be tested with a biochemical mixture containing an isolated mammalian Mus81-Eme1 endonuclease complex, a...

20060003435 - Production by yeasts of aspartic proteinases from plant origin with sheep's, cow's, goat's milk, etc. clotting and proteolytic activity: This invention is valid for recombinant enzymes produced from transformed yeast with coding genes for plant-origin aspartic acid proteinases. These proteinases have considerable sheep's, cow's and goat's milk clotting activity. They can be produced in large quantities by cultivating transformed yeast in a liquid medium. They are secreted into the...

20060003436 - Chamber with adjustable volume for cell culture and organ assist: The invention features modular chambers for culturing cells in which the volume of a chamber can be adjusted without compromising the seal or sterility of the chamber. The invention is based on the principle that the volume of a chamber formed between two plates sandwiching a compressible gasket and a...

20060003437 - Target detecting device and target capturer, device and method for molecular adsorption or desorption, and device and method for protein detection: The present invention provides, for example, a target detecting device comprising a target capturer, means for releasing the target capturer, light irradiating means and light detecting means, the target capturer at least partially containing a region interactive with an electrically conductive member, being capable of capturing a target, and being...

20060003440 - Device and process unit for providing a hybridization chamber: A device provides a hybridization chamber for hybridizing nucleic acid samples, proteins or tissue sections on a slide, and is an essentially rectangular body movable opposite the slide. The device includes a surface, lines for supplying and/or removing media, a specimen supply line and an agitating device. The agitation device...

20060003438 - Growth of inorganic thin films using self-assembled monolayers as nucleation sites: Systems and methods for preparing inorganic-organic interfaces using organo-transition metal complexes and self-assembled monolayers as organic surfaces. In one embodiment, a silicon wafer is cleaned and reacted with stabilized pirhana etch to provide an oxide surface. The surface is reacted with the trichlorosilyl end of alkyltrichlorosilanes to prepare self assembling...

20060003439 - Microfluidic system: The present invention provides microfluidic technology enabling rapid and economical manipulation of reactions on the femtoliter to microliter scale....

20060003442 - Cell culture scaffold, methods of manufacturing the scaffold and cell culture methods: A cell culture scaffold containing a gel having a network structure comprised of a synthetic polymer such that cultured cells spread in a shorter time and the number of adsorbed cultured cells per unit area is larger than in the case of using a gel having a network structure comprised...

20060003444 - Cells for detection of influenza and parainfluenza viruses: The invention provides cell lines which are useful for the rapid detection and production of influenza and parainfluenza viruses. In particular, the invention relates to transgenic mink lung cells which show increased sensitivity to infection by influenza A, influenza B, or parainfluenza 3 viruses, or which are capable of enhanced...

20060003441 - Method for cultivating cells, particularly human or animal cells: The invention relates to a method for cultivating cells of the most diverse type, particularly human or animal cells. According to the invention, a culture is prepared from cells of at least one specified type in a defined environment, and the cells of the relevant culture are supplied with assigned,...

20060003443 - Recombinase-expressing cells: In accordance with the present invention, there are provided cells for expressing recombinase Cre in the presence of recombinase FLP in a FLP-dependent manner, methods of expressing recombinase Cre by introducing recombinase FLP into the above cells, methods of producing recombinant viral vectors using cells that express recombinase Cre in...

20060003445 - Method and device for detecting feline immunodeficiency virus: A method and device for determining a feline immunodeficiency virus infection or vaccination in an animal. The method is an assay involving contacting a biological sample from a felid with an env FIV polypeptide and determining the binding of antibodies in the sample to the polypeptide. The assay is optimized...

20060003446 - Mesoderm and definitive endoderm cell populations: The present invention provides cell populations that are enriched for mesendoderm and mesoderm, and cell populations that are enriched for endoderm. The cell populations of the invention are useful for generating cells for cell replacement therapy....

20060003447 - Dry powder cells and cell culture reagents and methods of production thereof: The present invention relates generally to nutritive (e.g., cell culture) medium, medium supplement, media subgroup and buffer formulations. Specifically, the present invention provides powdered nutritive medium, medium supplement and medium subgroup formulations, particularly cell culture medium supplements (including powdered sera such as powdered fetal bovine serum (FBS)), medium subgroup formulations...

20060003448 - Dry powder cells and cell culture reagents and methods of production thereof: The present invention relates generally to nutritive (e.g., cell culture) medium, medium supplement, media subgroup and buffer formulations. Specifically, the present invention provides powdered nutritive medium, medium supplement and medium subgroup formulations, particularly cell culture medium supplements (including powdered sera such as powdered fetal bovine serum (FBS)), medium subgroup formulations...

20060003449 - Plant artificial chromosome compositions and methods: The present invention provides for the identification and cloning of functional plant centromeres in Arabidopsis. This will permit construction of stably inherited plant artificial chromosomes (PLACs) which can serve as vectors for the construction of transgenic plant and animal cells. In addition, information on the structure and function of these...

20060003450 - Lineage specific cells and phogenitor cells: A method for generating a culture that is purified or enriched in respect of cells of a selected lineage is described in which a selectable marker, which is differentially expressed in cells of the selected lineage compared with its expression in other cells, is introduced into a multipotential cell and...

20060003451 - Cell lines and constructs useful in production of e1-deleted adenoviruses in absence of replication competent adenovirus: Novel cell lines useful for trans-complementing E1-deleted adenoviral vectors are described. The cell lines are capable of providing high yields of E1-deleted adenoviral vectors in the absence of replication-competent adenovirus over multiple passages....

20060003453 - High-efficiency aav helper functions: The present invention provides methods and compositions for producing high titer preparations of recombinant AAV (“rAAV”) virions. The compositions of the present invention include AAV helper function systems and host cells. The present invention also includes methods of using AAV helper function vectors that effect the production of only small...

20060003452 - Vector packaging cell line: The invention relates to a method of increasing vector transduction in target cells. The invention provides for the recombinant engineering of a packaging cell line to be capable of expressing one or more membrane proteins which facilitate binding to, and activation of, a target cell. The invention also provides for...

20060003454 - Non-dividing donor cells for gene transfer: The present invention relates to compositions comprising conjugation-competent non-dividing cells for delivery of genes to bacterial cells by conjugation. The present invention also relates to methods of making, using, and storing such conjugation competent non-dividing cells....