Chemistry: molecular biology and microbiology inventions 12/05

20050287512 - Specimen storing device and method: A closed system for withdrawing, measuring, and isolating discrete quantities of liquid specimen for cryogenic preservation and recovery includes a fluoroplastic storage tube, an aspirating device, such as a syringe, and an impermeable barrier. The aspirating device can be used to meter exact amounts of specimen into the tube, and...

20050287514 - Compositions and methods useful for hcv infection: The present invention provides compositions comprising cells that can effectively produce HCV after HCV infection, compositions for culturing the cells, methods for making the composition and methods for infecting the cells in the composition with HCV. The present invention also provides methods for assaying HCV production and methods for evaluating...

20050287516 - Medium and method for preserving platelets, red blood cells, and other non-nucleus cells and platelets-containing composition: The present invention provides a platelet-containing composition prepared by contacting platelets with a medium for preserving. The medium comprises anticoagulant, cryoprecipitate and thrombin. The present invention also provides a method for long-term preservation of platelets, comprising the steps of: (a) adding anticoagulants, cryoprecipitate, thrombin in normal saline; (b) adding a...

20050287513 - Method for preserving intracellular molecular detail: A process and composition is described that allows the operator to fix and preserve tissue culture grown cells such that their intracellular molecular detail is retained for up to four years. This enables increased reproducibility of staining for antigens and small molecule targets for use in the areas of basic...

20050287515 - Removal of bacterial dna from therapeutic fluid formulations: This invention discloses methods and devices useful for eliminating or reducing the adverse effects associated with the use of a variety of therapeutic fluids. It is proposed herein that the source of such side effects (e.g. fever and septic shock) is the oligonucleotides present in the fluid and that using...

20050287518 - Labeled peptides, proteins and antibodies and processes and intermediates useful for their preparation: The invention provides peptide synthons having protected functional groups for attachment of desired moieties (e.g. functional molecules or probes). Also provided are peptide conjugates prepared from such synthons, and synthon and conjugate preparation methods including procedures for identifying the optimum probe attachment site. Biosensors are provided having environmentally sensitive dyes...

20050287519 - Methods, compositions and compound assays for inhibiting amyloid-beta protein production: A method for identifying compounds that inhibit amyloid-beta precursor protein processing in cells, comprising contacting a test compound with a KINASE polypeptide, or fragment thereof, and measuring a compound-KINASE property related to the production of amyloid-beta peptide. Cellular assays of the method measure indicators including phosphorylated kinase substrate and/or amyloid...

20050287520 - Screening method and device, and new drug screening method and device: The present invention realizes a screening method and device able to obtain the entire image of a cell as a detailed and accurate (with a good SN ratio) image at high speed by observing the cell image while a Nipkow system confocal scanner is used and the focal position of...

20050287517 - T1r hetero-oligomeric taste receptor: Newly identified mammalian taste-cell-specific G protein-coupled receptors which function as hetero-oligomeric complexes in the sweet taste transduction pathway, and the genes and cDNA encoding said receptors are described. Specifically, T1R G protein-coupled receptors active in sweet taste signaling as hetero-oligomeric complexes, and the genes and cDNA encoding the same, are...

20050287522 - Detection of protein translocation by beta-galactosidase reporter fragment complementation: Methods and compositions are provided for detecting molecular translocations, particularly protein translocations within and between subcellular copartments, using at least two components that exhibit a localization-dependent difference in complementation activity. In particular, alpha-complementing β-galactosidase fragments are provided. These β-galactosidase reporter fragments display significantly enhanced enzymatic activity when one fragment is...

20050287524 - Differential serological diagnosis of equine infectious anemia virus infected and vaccinated horses using recombinant s2 protein: We describe here the development and optimization of an embodiment of a new serologic EIAV diagnostic ELISA assay to detect serum antibodies to the EIAV S2 protein that are produced in infected horses, but not in horses inoculated with the EIAVUKΔS2 vaccine virus. An embodiment of the test S2 protein...

20050287523 - Functionalized platform for individual molecule or cell characterization: A system for characterization of a single molecule or cell sample by directing a beam onto the sample to produce energy emanating from the sample. A periodic sample holder with through-pores containing the sample is positioned to receive the beam. At least one pore is provided in the sample holder...

20050287521 - Oligonucleotides from sequences coding for the surface component of ptlv envelope proteins and uses thereof: The invention relates to the use of oligonucleotides from the nucleotide sequences coding for the amino-terminal region of the surface component (SU) of envelope proteins of PTLV viruses in order to perform methods of detecting every PTLV strain or PTLV-related viruses, e.g. for the detection of novel PTLV variants or...

20050287548 - Activatable probes and methods for in vivo gene detection: Probes for detecting a target polynucleotide are provided. One aspect provides a molecular beacon probe set wherein the donor molecular beacon comprises a quantum dot and an acceptor molecular beacon comprises at least one reporter. The probes optionally comprise a protein transduction domain, targeting signal, or a combination thereof. Methods...

20050287582 - Antibodies that specifically bind to fdf03: Nucleic acids encoding various monocyte cell proteins from a primate, reagents related thereto, including specific antibodies, and purified proteins are described. Methods of using said reagents and related diagnostic kits are also provided....

20050287550 - Bacterial counterselectable hybrid systems: The present application relates to the hybrid selection methods in prokaryotes using counterselectable reporter genes....

20050287535 - Biomarkers for wound healing: The invention relates to the discovery that in chronic wounds the expression levels of certain genes are altered relative to the expression levels observed in healthy tissues....

20050287588 - Breast cancer specific gene 1: The present invention relates to a novel BCSG1 protein. In particular, isolated nucleic acid molecules are provided encoding the human BCSG1 protein. BCSG1 polypeptides are also provided, as are vectors, host cells and recombinant methods for producing the same. Also provided are diagnostic methods for detecting breast cancer....

20050287552 - Carbohydrate encapsulated nanoparticle based affinity mass spectrometry: The present invention provides methods and compositions for carbohydrate encapsulated nanoparticle based mass spectrometry. For example, the present invention provides methods of screening samples for carbohydrate binding molecules, methods of characterizing carbohydrate binding epitopes in target molecules, and MALDI matrix compositions comprising carbohydrate encapsulated nanoparticles....

20050287543 - Diagnostic and therapeutic methods and compositions involving pten and breast cancer: Patients with ErbB2-overexpressing cancers can be given an ErbB2 targeting agent as a therapeutic regimen but not all patients are responsive. The present invention concerns the diagnostic, prognostic and therapeutic methods and compositions for evaluating potential efficacy of an ErbB2 targeting agent in an ErbB2-overexpressing cancers by evaluating PTEN expression,...

20050287536 - Disposable, multi-use, dna sample collection disk apparatus for field biohazard testing, dna testing, and personal authentication: An easy-to-use, versatile, disposable, customer-replaceable, multi-use, DNA sample collection disk apparatus for use with fixed, portable, or field-based DNA sample collection, analysis, and detection systems is disclosed. General features of the invention are its' small form factor, portability, wearability, ease-of-use, and self-contained capacity to collect and analyze multiple different human...

20050287547 - Embryonic stem cell markers and uses thereof: The present invention provides methods and reagents for identification, separation and characterization of embryonic stem cells using markers previously recognized as germ cell specific. One currently preferred marker is the DAZL marker now shown to be expressed in multipotent and pluripotent stem cells. Antibodies and other agents, capable of binding...

20050287571 - Epididymis-specific defensin for evaluating and regulating male fertility: The present invention relates to the discovery that an epididymis-specific defensin, EP2D, plays an important role in regulating sperm motility and therefore male fertility. Provided are methods for evaluating male fertility based on the level of EP2D bound to sperm, kits for evaluating male fertility, and methods for enhancing or...

20050287586 - Error detection in chemical array fabrication: A method, apparatus, and computer program product, for forming an addressable array of chemical moieties on a substrate. The method may include, for each of multiple locations on the substrate, depositing a reagent drop set during a cycle so as to attach a corresponding moiety for that location. This may...

20050287569 - Espfu nucleic acids and proteins and uses thereof: Disclosed are EspFU (EspF-like polypeptide encoded by a gene of the cryptic prophage CP-933U of enterohemorrhagic E. coli) polypeptides, fragments thereof, nucleic acids that encode EspFU polypeptides, or fragments thereof, and cells including the polypeptides, fragments, and/or nucleic acids. Also disclosed are model systems, kits, and methods for screening that...

20050287529 - Flea and tick octopamine receptor nucleic acid molecules, proteins and thereof: The present invention relates to tick octopamine receptor nucleic acid molecules; to tick octopamine receptor proteins encoded by such nucleic acid molecules; to antibodies raised against such proteins; and to compounds that inhibit the activity of such proteins. The present invention also includes methods to obtain such proteins, nucleic acid...

20050287538 - Frame-shifting pcr for germline immunoglobulin genes retrieval and antibody engineering: A method for preparing an antigen-specific antibody by constructing a library of phage-displayed single chain variable fragment of an antibody with a novel frame-shifting PCR is disclosed. Also disclosed is a method for preparing a clone for producing an antigen-specific antibody....

20050287544 - Gene expression profiling of colon cancer with dna arrays: Differential gene expression associated with histopathologic features of colorectal disease can be performed with nucleic acid arrays. Such arrays can comprise a pool of polynucleotide sequences from colon tissues, and the detection of the overexpression or underexpression of polynucleotide sequences (or subsequences or complements thereof) from this pool can provide...

20050287581 - Gene sequence of alpha(1,3)galactosyltransferase: Sequence data for the sheep α(1,3)galactosyltransferase (α1,3GT) gene is provided in this disclosure, which allows the user to construct vectors for expressing or inactivating the α1,3GT gene. This in turn enables the user to control expression of the Galα(1,3)Gal xenoantigen on the surface of cells....

20050287579 - Genes for male accessory gland proteins in drosophila melanogaster: The present invention provides a number of accessory gland proteins from Drosophila. The invention also provides an accessory gland protein which is toxic to insect cells and can be used to kill or inhibit the development of insects. Methods of controlling insects are also provided....

20050287574 - Genetic diagnostic method for scd risk stratification: A method of classifying individuals as to their risk of experiencing sudden cardiac death (SCD) utilizes single nucleotide polymorphisms (SNPs). A genotype profile, which includes one or more SCD-associated SNPs, is generated for an individual. The probability of experiencing SCD is determined based on the genotype profile....

20050287559 - Genetic polymorphisms associated with vascular, methods of detection and uses thereof: The present invention is based on the discovery of genetic polymorphisms that are associated with vascular diseases and in particular coronary artery disease and stenosis. In particular, the present invention relates to nucleic acid molecules containing the polymorphisms, variant proteins encoded by such nucleic acid molecules, reagents for detecting the...

20050287561 - Genomic proxy microarrays to identify microbial quantitative trait loci: Methods are provided for engineering microbial organisms to perform a desired function at higher levels than naturally existing strains. The diversity within and between species of the level of (a) genomic diversity and (b) performance of the desired function are used to identify genes that can be optimized for increasing...

20050287589 - High resolution dna detection methods and devices: The present invention provides methods and devices for detecting a target nucleic acid molecule. A set of oligonucleotide probes integrated into an electric circuit, where the oligonucleotide probes are positioned such that they can not come into contact with one another, are contacted with a sample. If the sample contains...

20050287576 - Identification, monitoring and treatment of disease and characterization of biological condition using gene expression profiles: A method provides an index that is indicative of the state of a subject, as to a biological condition, based on a sample from the subject. An embodiment of this method includes: deriving from the sample a profile data set, the profile data set including a plurality of members, each...

20050287580 - Isolating biological modulators from biodiverse gene fragment libraries: The present invention provides a method for identifying a modulator or mediator of a biological activity, which activity includes antigenicity and or immunogenicity, said method comprising the step of: (i) producing a gene fragment expression library derived from defined nucleotide sequence fragments; and (ii) assaying the expression library for at...

20050287567 - Jm-27 as a marker of benign prostatic hyperplasia: A serum assay for JM-27 identifies individuals having benign prostate hyperplasia (BPH). By means of the serum assay, methods of diagnosing BPH, methods of determining the severity of BPH, methods of monitoring the course of BPH therapy and methods of monitoring the progression of BPH are performed....

20050287573 - Lined multi-well plates: A multi-well plate includes a plurality of wells, and each well has a base and one or more side walls. A liner is located adjacent the base of at least one of the plurality of wells. A method for forming a multi-well plate system includes providing a multi-well plate and...

20050287577 - Method and apparatus for capturing target substance: A method for capturing a target substance in a sample, comprising the steps of preparing particles capable of capturing the target substance in a dispersed state in a fluid,aggregating the particles, and allowing the particles to capture the target substance in the sample by bringing the sample into contact with...

20050287560 - Method for preparing substrates having immobilized molecules and substrates: A method for the efficient immobilization of molecules onto substrate surfaces that employs an isocyanate compound to form a reactive isocyanate surface, nanoparticles onto surfaces as well as silylated molecules such as silylated oligonucleotides or proteins onto unmodified surfaces such as a glass surface is provided. Also provided are compounds,...

20050287553 - Method for the quantification of methylated dna: Particular aspects of the present invention provide a method for quantification of two different variations of a DNA sequence. Particularly, the invention relates to a quantification of methylated DNA, and for this purpose, the test DNA is converted so that cytosine is converted to uracil, while 5-methylcytosine remains unchanged. The...

20050287549 - Method of genetic testing: This invention provides a method of genetic testing that enables testing of a plurality of variation sites (SNPs) in a cost-effective and simple manner, allowing realization of genetic diagnosis in clinical settings. The SNP type of the nucleic acid sample is evaluated by: allowing a nucleic acid sample having an...

20050287539 - Methods and compositions for preparing capped rna: The present invention concerns methods and compositions for increasing the yield of capped and full-length RNA transcripts produced in in vitro transcription reactions. Such methods and compositions can be used for cost-efficient, large scale production of capped full-length RNA transcripts that can be subsequently translated. Methods and compositions involve reaction...

20050287583 - Methods and kits for isolating biological target materials using silica magnetic particles: The present invention provides methods for isolating biological target materials, particularly nucleic acids, such as DNA or RNA or hybrid molecules of DNA and RNA, from other substances in a medium using silica magnetic particles. The methods of the present invention involve forming a complex of the silica magnetic particles...

20050287531 - Methods and systems for inferring bovine traits: Methods, compositions, and systems are provided for managing bovine subjects in order to maximize their individual potential performance and edible meat value, and to maximize profits obtained in marketing the bovine subjects. The methods and systems draw an inference of a trait of a bovine subject by determining the nucleotide...

20050287528 - Methods for detecting ovarian cancer: A method for detecting a kallikrein 8 polypeptide associated with ovarian cancer in a patient is provided comprising detecting kallikrein 8 polypeptide in a sample from the patient. Methods are also provided for monitoring the progression of ovarian cancer in a patient, determining whether ovarian cancer has metastasized or is...

20050287557 - Methods for identifying compounds for treating diabetes mellitus: The invention is directed to methods for identifying candidate beta cell-sensitizing compounds comprising providing a pancreatic beta islet cell population derived from a diabetic subject, contacting the beta islet cell population with one or more somatostatin receptor 5-binding compounds, and identifying those somatostatin receptor 5-binding compounds that promote insulin secretion...

20050287532 - Methods for monitoring drug activities in vivo: Methods, systems and equipment useful for monitoring in vivo activities of CCI-779 or other drugs. Numerous drug activity genes can be identified by the present invention. The expression profiles of these genes in peripheral blood mononuclear cells can be modulated by CCI-779 or other drugs. Therefore, these genes can be...

20050287578 - Methods for rna fluorescence in situ hybridization: The present invention provides improved methods for in situ hybridization (ISH) comprising: (a) obtaining a tissue sample from a subject; (b) contacting the tissue sample with a fixative under conditions to cause fixation of the tissue sample; (c) contacting nucleic acids in the tissue sample with a detectable probe under...

20050287565 - Methods, compositions and compound assays for inhibiting amyloid-beta protein production: A method for identifying compounds that inhibit amyloid-beta precursor protein processing in cells, comprising contacting a test compound with a GPCR polypeptide, or fragment thereof, and measuring a compound-GPCR property related to the production of amyloid-beta peptide. Cellular assays of the method measure indicators including second messenger and/or amyloid beta...

20050287541 - Microarray for predicting the prognosis of neuroblastoma and method for predicting the prognosis of neuroblastoma: A microarray for predicting the prognosis of neuroblastoma, wherein the microarray has 25 to 45 probes related to good prognosis, which are hybridized to a gene transcript whose expression is increased in a good prognosis patient with neuroblastoma and are selected from 96 polynucleotides consisting of the nucleotide sequences of...

20050287585 - Microarray synthesis and assembly of gene-length polynucleotides: There is disclosed a process for in vitro synthesis and assembly of long, gene-length polynucleotides based upon assembly of multiple shorter oligonucleotides synthesized in situ on a microarray platform. Specifically, there is disclosed a process for in situ synthesis of oligonucleotide fragments on a solid phase microarray platform and subsequent,...

20050287572 - Microfabricated integrated dna analysis system: Methods and apparatus for genome analysis are provided. A microfabricated structure including a microfluidic distribution channel is configured to distribute microreactor elements having copies of a sequencing template into a plurality of microfabricated thermal cycling chambers. A microreactor element may include a microcarrier element carrying the multiple copies of the...

20050287590 - Multi-functional microarrays and methods: Detection devices for multianalyte detection on a solid substrate, methods for the preparation of the devices and their use in analytical and diagnostic procedures are described. The detection devices include a solid substrate fabricated with an array of detection spots, the detection spots having an analyte sensor bound to the...

20050287562 - Novel human kinase proteins and polynucleotides encoding the same: Novel human polynucleotide and polypeptide sequences are disclosed that can be used in therapeutic, diagnostic, and pharmacogenomic applications....

20050287568 - Novel human transferase proteins and polynucleotides encoding the same: Novel human polynucleotide and polypeptide sequences are disclosed that can be used in therapeutic, diagnostic, and pharmacogenomic applications....

20050287546 - Novel proteases: The present invention relates to protease polypeptides, nucleotide sequences encoding the protease polypeptides, as well as various products and methods useful for the diagnosis and treatment of various protease-related diseases and conditions....

20050287591 - Nucleic acid amplification and detection method: Method of amplifying and detecting a nucleic acid target molecule contained in a test sample using a device having amplification primers and detectably labeled hybridization probes immobilized on the same surface of a solid support. Soluble amplicons produced in an in vitro nucleic acid amplification reaction employing the immobilized amplification...

20050287526 - Nucleic acid amplification method: A nucleic acid amplification method, and probes for use within the method are described....

20050287563 - Nucleic acid labeling compounds: Nucleic acid labeling compounds containing heterocyclic derivatives are disclosed. The heterocyclic derivative containing compounds are synthesized by condensing a heterocyclic derivative with a cyclic group (e.g. a ribofuranose derivative). The labeling compounds are suitable for enzymatic attachment to a nucleic acid, either terminally or internally, to provide a mechanism of...

20050287566 - Oligonucleotide analogues: The present invention relates to novel bicyclic and tricyclic nucleoside and nucleotide analogues as well as to oligonucleotides comprising such elements. The nucleotide analogues, LNAs (Locked Nucleoside Analogues), are able to provide valuable improvements to oligonucleotides with respect to affinity and specificity towards complementary RNA and DNA oligomers. The novel...

20050287534 - Primers and probe to identify mycobacterium tuberculosis complex: Methods and nucleic acids for rapid, reliable and sensitive detection of Mycobacterium tuberculosis (MTB) complex pathogen in a biological sample. Oligonucleotides are provided which amplify MTB DNA and which are useful in carrying out real time PCR of DNA obtained from formalin-fixed and paraffin-embedded tissue samples....

20050287570 - Probe arrays for expression profiling of rat genes: The present invention provides probe arrays for expression profiling of rat genes. Each probe array comprises a plurality of probes, each of which is directed to a rat gene that encodes a sequence selected from SEQ ID NOs: 1-8,192. Suitable probes for the present invention include polynucleotides that can hybridize...

20050287540 - Production of attenuated negative stranded rna virus vaccines from cloned nucleotide sequences: Attenuated, recombinant negative stranded RNA viruses suitable for vaccine use are produced from one or more isolated polynucleotide molecules encoding the virus. A recombinant genome or antigenome of the subject virus is modified to encode a mutation within a recombinant protein of the virus at one or more amino acid...

20050287587 - Production of glycosylated macrolides in e. coli: Methods and materials are provided for E. coli host cells containing an expression system for producing NDP 6-deoxy-sugar, which may also comprise an expression system for expressing 6-deoxyglycosyl transferase and/or an expression system for producing a polyketide and/or polyketide oxidation enzymes....

20050287545 - Protein belonging to the tnf superfamily involved in signal transduction, nucleic acids encoding same, and methods of use thereof: A method of modulating immune response in an animal is disclosed. Such a method interacting the immature dendritic cells from the animal with an antigen ex vivo so that the immature dendritic cells present the antigen on their surfaces, inducing maturation of the immature dendritic cells ex vivo, and contacting...

20050287542 - Protein binding miniature proteins: The present invention provides a protein scaffold, such as an avian pancreatic polypeptide, that can be modified by substitution of two or more amino acid residues that are exposed on the alpha helix domain of the polypeptide when the polypeptide is in a tertiary form....

20050287537 - Pullulanase variants and methods for preparing such variants with predetermined properties: The present invention relates to pullulanase variants, wherein the variants have improved properties, for example, altered pH optimum, improved thermostability, altered substrate specificity, increased specific activity or altered cleavage pattern....

20050287527 - Quantitative ranking of transient ligand binding to target biomolecules: There is provided a method of quantitatively ranking transient ligand binding to target biomolecules by means of NMR relaxation dispersion profiles. The present invention also relates to a method to identify ligand site obeying two-state and more complex binding behavior in a transient complex of a ligand with a target...

20050287554 - Regions of papilloma virus e1 helicase involved in e1 oligomerization: There is provided an amino acid sequence comprised within the PV E1 protein region A delineated by amino acids 352 and 439, and any derivative variant or fragment thereof, necessary for the oligomerization of the E1 protein. This amino acid sequence is capable of self-association and of associating with the...

20050287584 - Secreted proteins encoded by human chromosome 13: The present invention relates to polynucleotide and polypeptide molecules a for zsig46 polypeptide, a novel secreted protein located on human chromosome 13. The zsig46 polypeptides, and polynucleotides encoding them, are secreted proteins and may be used in the study of receptors for which a ligand has not yet been identified,...

20050287558 - Snps of apolipoprotein b and modulation of their expression: Compounds, compositions and methods are provided for modulating the expression of apolipoprotein B. The compositions comprise oligonucleotides, targeted to nucleic acid encoding apolipoprotein B. Methods of using these compounds for modulation of apolipoprotein B expression and for diagnosis and treatment of diseases and conditions associated with expression of apolipoprotein B...

20050287551 - Susceptibility gene for human stroke; methods of treatment: A role of the human PDE4D gene in stroke is disclosed. Methods for diagnosis, prediction of clinical course and treatment for stroke using polymorphisms in the PDE4D gene are also disclosed....

20050287575 - System and method for improved genotype calls using microarrays: An embodiment of a method for calling the genotype of a biological sequence is described that comprises receiving sets of intensity data each comprising an intensity value for each probe feature associated with a probe set disposed on a probe array; independently applying filters to the intensity values of a...

20050287533 - Target-specific compomers and methods of use: The present invention provides a novel class of molecules, termed “compomers,” that enable the indirect detection of target molecules, as well as novel target detection reagents and compomer templates that encode compomers. Compomers are linear polymers generated from the compomer template portion of a target detection reagent during the course...

20050287530 - Tcl-1b gene and protein and related methods and compositions: The TCL1 gene family, located on the human chromosome at the 14q32.1 locus, are implicated in the development of T-cell malignancies. The present invention discloses the identification and characterization of new members of this gene family, the TCL-1b, TNG1 and TNG2 genes. The TCL-1b, TNG1 and TNG2 gene sequences are...

20050287592 - Template-dependent nucleic acid polymerization using oligonucleotide triphosphates building blocks: A novel use of a template-dependent polymerase. The novel use is effected by employing the template-dependent polymerase for incorporating at least one oligonucleotide triphosphate onto a nascent oligonucleotide-3′-OH in a template-dependent manner....

20050287564 - Therapeutic polypeptides, nucleic acids encoding same, and methods of use: Disclosed herein are nucleic acid sequences that encode G-coupled protein-receptor related polypeptides. Also disclosed are polypeptides encoded by these nucleic acid sequences, and antibodies, which immunospecifically-bind to the polypeptide, as well as derivatives, variants, mutants, or fragments of the aforementioned polypeptide, polynucleotide, or antibody. The invention further discloses therapeutic, diagnostic...

20050287525 - Tsg101 as inhibitors of hiv production: The present invention provides methods for identifying peptides in a mammalian. Tsg101 protein that binds to the PTAPP motif or L domain of human immunodeficiency virus type I (HIV-1). Such peptides can be used to inhibit Tsg101-HIV Gag binding, and is therefore effective in reducing HIV particle production. The invention...

20050287593 - Use of cytokine expression to predict skin inflammation; methods of treatment: Provided are methods for diagnosing the propensity of a subject to develop skin inflammation, in particular, psoriasis. Also provided are methods of treatment with antagonists of IL-17, IL-19, and/or IL-23....

20050287556 - Use of hla-g genotyping in immune-mediated conditions: Assays, including those available in kits, are used to obtain HLA-G genotypes. Kits provide reagents and devices to type biological samples for HLA-G through use of SNPs. Genotypes are predictors of risk....

20050287555 - Uses of cationic salts for polynucleotide synthesis: Inkjet printhead solvents and methods of forming an addressable nucleotide array are disclosed....

20050287599 - Biopolymer marker indicative of disease state having a molecular weight of 1518 daltons: The instant invention involves the use of a combination of preparatory steps in conjunction with mass spectroscopy and time-of-flight detection procedures to maximize the diversity of biopolymers which are verifiable within a particular sample. The cohort of biopolymers verified within such a sample is then viewed with reference to their...

20050287597 - Compositions and methods for modulating sirtuin activity: The present invention provides treatment methods involving modulating a sirtuin activity and/or a sirtuin mRNA and/or a sirtuin polypeptide level. In some embodiments, the present invention provides treatment methods involving modulating SIRT1 activity and/or SIRT mRNA and/or polypeptide level. The present invention provides methods of inhibiting SIRT1 Tat deacetylase activity....

20050287601 - Diagnostics and therapeutics for macular degeneration-related disorders: The invention relates to methods for diagnosing and treating macular degeneration-related disorders. The invention also related to methods for identifying genes that cause macular degeneration-related disorders....

20050287595 - Macroglobulin biopolymer markers indicative of insulin resistance: The instant invention involves the use of a combination of preparatory steps in conjunction with mass spectroscopy and time-of-flight detection procedures to maximize the diversity of biopolymers which are verifiable within a particular sample. The cohort of biopolymers verified within such a sample is then viewed with reference to their...

20050287600 - Method for screening for agents preventing aggregation of potentially amyloidogenic proteins: The present invention describes a method for identifying compounds capable of preventing the aggregation of potentially amyloidogenic proteins. The invention encompasses (1) missing the protein of interest with the compound to be assayed (b) gradually increasing the temperature (c) detecting the appearance of amyloid fibres by turbidimetric measurements, and/or by...

20050287598 - Method of preparing cow brain homogenate: An assay method is disclosed which isolates and detects the presence of a disease related conformation of a protein (e.g., PrPSc) present in a sample also containing the non-disease related conformation of the protein (e.g., PrPC). The sample is treated (e.g., contacted with protease) in a manner which hydrolyzes the...

20050287596 - Novel ligands and libraries of ligands:

20050287594 - Proteomic analysis: The present invention provides methods for analyzing proteomes, as cells or lysates. The analysis is based on the use of probes that have specificity to the active form of proteins, particularly enzymes and receptors. The probes can be identified in different ways. In accordance with the present invention, a method...

20050287605 - Caspase 3 inhibitors: The invention is intended to develop a drug capable of directly or indirectly inhibiting the activation of caspase. Specifically, the invention provides a caspase 3 inhibitor comprising a protein comprising the same or substantially the same amino acid sequence as that represented by SEQ ID NO: 1, 2, 3 or...

20050287604 - Maternal antibodies as fetal cell markers to identify and enrich fetal cells from maternal blood: The present invention provides methods for identifying and/or enriching fetal cells from maternal blood, using as fetal cell markers the antibodies that the mother produces against paternally inherited fetal antigens. The fetal cell-maternal antibody complexes are identified and isolated using labelled agents that bind to the maternal antibodies. The present...

20050287602 - Method of identifying transmembrane protein-interacting compounds: A method for screening compounds for their ability to interact with transmembrane proteins is provided. Also provided is a method for determining whether proteins such as transmembrane proteins are able to oligomerise....

20050287603 - Modulation of bone development: Methods and compositions for modulating bone development are described....

20050287606 - T1 receptor-like ligand i: The present invention concerns a novel T1R-like ligand I protein. In particular, isolated nucleic acid molecules are provided encoding the T1R-like ligand I protein. T1R-like ligand I polypeptides are also provided, as are recombinant vectors and host cells for expressing the same....

20050287609 - Methods and compositions for detection of equine tapeworm infections: Methods for detection of a tapeworm in an animal are provided, comprising contacting a biological sample from the animal with at least one antibody capable of binding to an antigen derived from Anoplocephala perfoliata, allowing an antigen-antibody complex to form, separating the biological sample and the antigen-antibody complex, and detecting...

20050287610 - Method for detecting the presence or risk of prostate cancer by detecting products of psa or klk2: The present invention discloses a method for detecting the presence or diagnosing the risk of cancer, especially prostate cancer, either before or after the onset of clinical symptoms, by detecting a level or functional activity of an aberrant expression product of a gene selected from PSA or KLK2, which correlates...

20050287611 - Mhc bridging system for detecting ctl-mediated lysis of antigen presenting cells: A bridging assay that utilizes a multivalent MHC binding molecule to enumerate the number of antigen-specific CTLs in a particular sample and also determines the functional capability of the CTL population in the sample is provided. In one embodiment, the assay is used to measure the effector function of any...

20050287612 - Card-4 molecules and uses thereof: The invention provides methods of using CARD4 molecules to screen for modulators of LPS-induced cell signaling pathways. Also included are CARD-4 deficient mice, methods of modulating LPS-induced cell signaling pathways, and methods of treating or preventing bacterial infections....

20050287607 - Methods and compositions for identification of antigen-specific b cell populations: Provided are compositions and methods for identifying, enumerating and isolating B cells that react to a particular antigen. Included are tetrameric antigen-avidin complexes not comprising an MHC molecule, kits for producing the complexes, and reagents for diagnosing an autoimmune disease or monitoring progression of an autoimmune disease comprising the complexes....

20050287608 - Diagnostic methods for protein profiling: Methods for establishing a protein expression profile of a biological sample, by contacting a biological sample with a ubiquitin-binding protein such that ubiquitinated and specific non-ubiquitinated protein (the IPS) bind to the ubiquitin-binding protein; isolating the IPS proteins; and analyzing the isolated IPS proteins, wherein an expression profile is generated....

20050287614 - Assay for detecting an analyte containing or labelled with a haem moiety: A method for detecting an analyte containing or labelled with a haem moiety within a sample, said method comprising: a) contacting said sample with a magnetic bead having immobilised thereon a specific binding partner for said analyte and allowing analyte to bind to said specific binding partner; b) separating the...

20050287617 - Inhibitors of macrophage migration inhibitory factor and methods for identifying the same: m

20050287618 - Labeling immobilized proteins with dipyrrometheneboron difluoride dyes: The invention describes methods for labeling or detecting of immobilized poly(amino acids), including peptides, polypeptides and proteins, on membranes and other solid supports, using fluorescent dipyrrometheneboron difluoride dyes. Such immobilized poly(amino acids) are labeled or detected on blots or on arrays of poly(amino acids), or are attached to immobilized aptamers....

20050287616 - Method of assaying denaturation of proteins: The present invention encompasses a method for performing an antibody-antigen assay using an ion-exchange technique prior to performing an antibody-antigen assay. Actives from protein-denaturing solutions are physically removed from the test sample, preferably by using an ion-exchange resin. The present invention also includes a method of performing an antibody-antigen assay,...

20050287613 - Polyclonal-polyclonal elisa assay for detecting n-terminus-probnp: A specific and sensitive in vitro ELISA assay and diagnostic test kit is disclosed for determining levels of NT-proBNP protein in a variety of bodily fluids, non-limiting examples of which are blood, serum, plasma, urine and the like. The NT-proBNP ELISA assay test employs the sandwich ELISA technique to measure...

20050287615 - Screening assay to identify inhibitors of the murd enzyme using an activator-independent murd enzyme: A method of identifying inhibitors of an activator-independent MurD enzyme comprising contacting the enzyme with a test compound in the presence of enzyme substrates and appropriate buffers and detecting any modulation of enzyme activity by the test compound....

20050287619 - Method and reagent for measuring cholesterol in high-density lipoproteins: A method for quantitatively determining cholesterol in high-density lipoprotein, which comprises: treating a sample with cholesterol esterase and cholesterol oxidase or cholesterol esterase, an oxidized coenzyme and cholesterol dehydrogenase in an aqueous medium comprising a bile acid derivative; and measuring the formed hydrogen peroxide or a reduced coenzyme; and a...

20050287620 - Method of determining analyte level using subcutaneous electrode: A small diameter flexible electrode designed for subcutaneous in vivo amperometric monitoring of glucose is described. The electrode is designed to allow “one-point” in vivo calibration, i.e., to have zero output current at zero glucose concentration, even in the presence of other electroreactive species of serum or blood. The electrode...

20050287621 - Chip and apparatus for acetylcholinesterase inhibitor detection and method for using thereof: A chip and an apparatus for acetylcholinesterase inhibitor detection and a method for using thereof are provided. The chip of the invention comprises a substrate and a film, coated on said substrate, including a molecule with a specific ability to combine with an acetylcholinesterase inhibitor. The present invention utilizes the...

20050287622 - High throughput assays for the proteolytic activities of clostridial neurotoxins: In this application is described substrates for high-throughput assays of clostridial neurotoxin proteolytic activities. Two types of substrates are described for use in assays for the proteolytic activities of clostridial neurotoxins: (1) modified peptides or proteins that can serve as FRET substrates and (2) modified peptides or proteins that can...

20050287623 - Method for detecting reactive metabolites using stable-isotope trapping and mass spectroscopy: The invention is directed to a method for detecting reactive metabolites using isotope trapping and mass spectroscopy....

20050287624 - Modified sarcosine oxidases, modified sarcosine oxidase genes, and methods for preparing the modified sarcosine oxidases: A modified sarcosine oxidase having a lowered activity for N-ethylglycine. Such a modified oxidase may have the following physicochemical properties: (a) action: hydrolyzes 1 mol of sarcosine to produce 1 mol of glycine and 1 mol of formaldehyde; (b) substrate specificity: reactivity for N-ethylglycine is 70% or less compared with...

20050287625 - Process for expression of foreign genes in methylotrophic bacteria through chromosomal integration: Provided is a method for expressing an introduced gene or genes in a C1 metabolizing microorganism host wherein the gene(s) are integrated into the tig region of the chromosome. This method provides high level expression in a stable manner in which growth rate of the host strain is not highly...

20050287626 - Process for producing dipeptides: The present invention provides a process for producing a dipeptide which comprises culturing in a medium a microorganism which has the ability to produce a protein having the activity to form the dipeptide from one or more kinds of amino acids and which has the ability to produce at least...

20050287627 - Process for producing dipeptides or dipeptide derivatives: The present invention provides a process for producing a dipeptide or a dipeptide derivative using a phosphate donor, a substance selected from the group consisting of adenosine-5′-monophosphate, adenosine-5′-diphosphate and adenosine-5′-triphosphate, one or more kinds of amino acids or amino acid derivatives, and as enzyme sources, a protein having polyphosphate kinase...

20050287629 - Class ii human histone deacetylases, and uses related thereto: The invention provides histone deacetylase class II nucleic acids and polypeptides, methods and reagents for their use, and related compounds including small molecule libraries containing class II histone deacetylase inhibitors....

20050287631 - Compositions and methods related to a dimeric mhc class i and ii-like molecule (dsmhci and dsmhcii): Embodiments of the invention include dimeric soluble MHC molecules (dsMHCs) and compositions and methods for their use. The molecules may be used to downregulate activated T cells. The dsMHCs may be administered by intraperitoneal infusion into a tissue or an organ recipient. The administration of dsMHCs molecules may also be...

20050287642 - Efficient protein expression system: Nucleic acid expression control sequence cassettes and vectors containing the same are provided for use in making abundant quantities of recombinant polypeptides of interest. The modified transcriptional control sequences, which include a T5 promoter sequence, are highly stable and can be used in a variety of vectors, such as plasmids....

20050287637 - Engineering intracellular sialylation pathways: Methods for manipulating carbohydrate processing pathways in cells of interest are provided. Methods are directed at manipulating multiple pathways involved with the sialylation reaction by using recombinant DNA technology and substrate feeding approaches to enable the production of sialylated glycoproteins in cells of interest. These carbohydrate engineering efforts encompass the...

20050287641 - Gene encoding a nonribosomal peptide synthetase for the production of ramoplanin: The present invention relates to isolated genetic sequences encoding nonribosomal peptide synthetase (NRPS) proteins which direct the biosynthesis of the antibiotic ramoplanin in microorganisms such as Actinoplanes sp. The isolated gene sequences serve as substrates for bioengineering of antibiotic structures....

20050287630 - Human antibodies derived from immunized xenomice: Fully human antibodies against a specific antigen can be prepared by administering the antigen to a transgenic animal which has been modified to produce such antibodies in response to antigenic challenge, but whose endogenous loci have been disabled. Various subsequent manipulations can be performed to obtain either antibodies per se...

20050287640 - Human spasmolytic polypeptide in glycosylated form: l

20050287638 - Hyaluronan receptor for endocytosis, variants thereof, and methods of making and using same: A purified recombinant mammalian HARE comprising a polypeptide which is able to specifically bind at least one of HA, chondroitin and chondroitin sulfate is disclosed, as well as methods of expressing and using same. Also disclosed are functionally active variants of HARE which are able to specifically bind at least...

20050287639 - Methods of incorporating amino acid analogs into proteins: The invention provides a method of incorporating nonstandard amino acids into a protein by utilizing a modified aminoacyl-tRNA synthetase to charge the nonstandard amino acid to a modified tRNA, which forms strict Watson-Crick base-pairing with a codon that normally forms wobble base-pairing with natural tRNAs....

20050287634 - Novel genes of cell wall-degrading enzymes derived from aspergillus, and method for the production the enzymes: The purpose of the present invention is to isolate a gene encoding novel exo-, or endo-β-1,3-glucanase, to obtain a microorganism having an enhanced expression of said gene and to degrade β-1,3-glucan to its low molecular weight form by means of said enzymes. The present invention relates to a gene or...

20050287632 - Peptide amidation process: The invention provides a process for amidating a desired peptide comprising cleaving a substrate polypeptide at a X1-cysteine sequence, wherein X1 is the amino acid at the peptide carboxyl-terminus and cysteine is the first amino acid of a palladium cleavage site comprising the sequence cysteine-X2-X3, wherein X2 is any amino...

20050287636 - Proapolipoproteina-i mutant and pharmaceutical composition comprising the same for prevention and treatment of atherosclerosis and hyperlipidemia: The proapoA-I mutants of the present invention exhibit superior LDL-antioxidant activities over the wild-type proapoA-I and higher efficiencies for delivering cholesterol to hepatocytes than the previously reported apoA-I-R173C, and thus they can be effectively used for prevention and treatment of hyperlipidemia or atherosclerosis....

20050287635 - Rtd receptor: Novel polypeptides, designated RTD, which are capable of binding Apo-2 ligand are provided. Compositions including RTD chimeras, nucleic acid encoding RTD, and antibodies to RTD are also provided....

20050287628 - System and method for the production of recombinant glycosylated proteins in a prokaryotic host: A system and a method for the production of recombinant N-glycosylated target proteins. The system comprises a prokaryotic organism (e.g. Escherichia coli) into which is introduced a genetic information encoding for a metabolic apparatus capable of carrying out the requested N-glycosylation of the target protein. Said prokaryotic organism also contains...

20050287633 - Vanilloid receptor: The isolated nucleic acid encoding human vanilloid receptor, said receptor, its preparation, cells expressing said receptor and an assay for testing compounds for their potential to decrease pain in humans. The receptor is involved in detection of noxious stimuli in mammalian organisms....

20050287643 - Protein binding miniature proteins and uses thereof: In certain aspects, the present invention provides miniature proteins resulted from a protein scaffold such as an avian pancreatic polypeptide that can be modified by substitution of at least one amino acid residue. In other aspects, the present invention provides diagnostic and therapeutic uses of these miniature proteins....

20050287644 - Methods for producing and culturing rabbit-mouse hybridomas and monoclonal antibodies secreted by rabbit-mouse hybridomas: Methods for producing rabbit-mouse hybridomas are provided. Methods for culturing rabbit-mouse hybridomas are provided. Monoclonal antibodies secreted from rabbit-mouse hybridomas are provided. Epitopes recognized by anti-PRL-3 antibodies are provided....

20050287645 - Overproduction hosts for biosynthesis of polyketides: Generic overproduction host cells can be used to produce any polyketide and obviate the need for performing conventional strain improvement....

20050287646 - Streptococcus equisimilis hyaluronan synthase gene and expression thereof in bacillus subtilis: The present invention relates to a nucleic acid segment having a coding region segment encoding enzymatically active Streptococcus equisimilis hyaluronate synthase (seHAS), and to the use of this nucleic acid segment in the preparation of recombinant cells which produce hyaluronate synthase and its hyaluronic acid product. Hyaluronate is also known...

20050287647 - Plant artificial chromosomes, uses thereof and methods of preparing plant artificial chromosomes: Methods for preparing cell lines that contain plant artificial chromosomes, methods for preparation of plant artificial chromosomes, methods for targeted insertion of heterologous DNA into plant artificial chromosomes, and methods for delivery of plant chromosomes to selected cells and tissues are provided. In particular, plant artificial chromosomes that are substantially...

20050287648 - Protein transducing domain/deaminase chimeric proteins, related compounds, and uses thereof: Disclosed are compositions for chimeric proteins comprising a protein transduction domain and a deaminase domain, mimetics or analog thereof, and uses of same....

20050287649 - Animal component free meningococcal polysaccharide fermentation and seedbank development: Animal-free meninge fermentation media and process is developed based upon use of a chemically defined medium. To improve polysaccharide production, fed-batch fermentation is examined using different feed solutions and feeding strategies. A feed solution containing glucose, amino acids, and trace metal elements produces Group A polysaccharide at approximately 3 times...

20050287650 - Process for the preparation of 2,4-dideoxyhexoses and 2,4,6-trideoxyhexoses: The invention relates to a process for the preparation of a 2,4-dideoxyhexose or a 2,4,6-trideoxyhexose from a substituted or unsubstituted carbonyl compound with at least 2 carbon atoms and at least one α-hydrogen atom and a substituted or unsubstituted aldehyde in the presence of an aldolase and water, the reaction...

20050287652 - Delta-8 desaturase and its use in making polyunsaturated fatty acids: Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding a delta-8 desaturase along with a method of making long chain polyunsaturated fatty acids (PUFAs) using this delta-8 desaturase in plants and oleaginous yeast....

20050287651 - Process for producing microbial fat or oil having lowered unsaponifiable matter content and said fat or oil: A process for producing a crude oil having lowered unsaponifiable matter content and/or ester-type sterol content and comprising a highly unsaturated fatty acid as a constituent fatty acid, characterized in that a microorganism capable of producing a fat or oil comprising an unsaturated fatty acid as a constituent fatty acid...

20050287654 - Process for the solvent-based extraction of polyhydroxyalkanoates from biomass: Improved processes for extracting polyhydroxyalkanoate from a biomass containing the polyhydroxyalkanoate including the steps of: combining the biomass containing the polyhydroxyalkanoate with a solvent selected from lower chain ketones, and mixtures thereof to form a biomass liquor wherein the biomass liquor comprises less than about 25% water; mixing the biomass...

20050287653 - Solvent extraction of polyhydroxyalkanoates from biomass: Processes for the extraction of PHAs from biomass, said processes comprising: combining the biomass containing the PHAs with a single main solvent to form a biomass liquor; heating the biomass liquor to at least partially solubilize the PHAs from the biomass to form a PHA liquor; separating the biomass from...

20050287655 - Process for producing mevalonic acid: A microorganism having the ability to biosynthesize mevalonic acid from acetyl-CoA is obtained by transfecting a DNA participating in biosynthesis of mevalonic acid from acetyl-CoA into a host microorganism, preferably into a microorganism having only a non-mevalonic acid pathway, to express the DNA therein. Mevalonic acid can be efficiently produced...

20050287656 - Mutated alkaline cellulase: A mutated alkaline cellulase derived from a cellulase having the amino acid sequence represented by SEQ ID NO:1 or one having a homology of at least 90% therewith by deleting one or more amino acid residues from the 343- to 377-positions in SEQ ID NO:1 or a region corresponding thereto...

20050287657 - Method for producing recombinant adenovirus: The invention concerns a method for producing recombinant adenovirus by which viral DNA is introduced in a packaging cell culture and the viruses produced are harvested after liberation in the supernatant. The invention also concerns the viruses produced and their use....

20050287658 - Drainage bioremediation apparatuses and methods: The method of the dual chamber bioremediation apparatus involves preparing a waste drain dosing batch of bioremedial bacteria in one chamber of the apparatus while dispensing another batch from a second chamber, and alternately switching the chamber functions. The enhanced replication bioremedial apparatus comprises a bioremedial pellet dispensing wheel, an...

20050287659 - System and method for separating biomass from media in a fluidized bed reactor: A system is provided for separating biomass from media in a fluidized bed reactor containing an aqueous suspension of biomass and media. A lift, configured to extend within the reactor, has an inlet for receiving aqueous suspension and an outlet spaced from the inlet for delivering the aqueous suspension from...

20050287660 - Scalable bioreactor culture process and system for the maturation of conifer somatic embryos: A bioreactor culture system and process for producing conifer somatic embryos comprise a closed vessel, a biomass immobilization matrix, a liquid culture medium recirculating equipment, and a gas control equipment. The biomass immobilization matrix is installed in a closed vessel, a liquid culture medium is introduced in the closed vessel,...

20050287661 - Methods and systems for multiplexing ir-mediated heating on a microchip: The present invention relates to methods and systems for rapid multiplexed heating of a plurality of small volume samples on a microchip. More specifically, the present invention relates to methods and systems for non-contact temperature cycling of the samples using infrared (IR)-mediated heating of small, micro to nanoliter, volume samples,...

20050287662 - Helper-free stocks of recombinant adeno-associated virus vectors: The present invention relates to a method for producing helper-free stocks of recombinant adeno-associated virus (rAAV) which can be used to efficiently and stably transduce foreign genes into host cells or organisms. The method comprises the cotransfection of eukaryotic cells with rAAV and with helper AAV DNA in the presence...

20050287663 - Dna encoding human alpha and beta subunits of neuronal nicotinic acetylcholine receptor, cells transformed therewith, and a recombinant cell line expressing a functional human alpha7 subunit of neuronal nicotinic acetylcholine receptor: Isolated nucleic acid molecules, i.e., DNA or RNA encoding human neuronal nicotinic acetylcholine receptor α and β subunits, mammalian and amphibian cells containing said DNA, methods for producing α and β subunits and recombinant (i.e., isolated or substantially pure) α subunits and β subunits are provided. In addition, cells expressing...

20050287664 - Cellular proliferation control factors: We discovered that Shen-Dan (SD), and its ligand HEDJ/ERdj3, are important for embryonic stem cell proliferation. These factors also promote neoplastic cell proliferation. Accordingly, compositions comprising SD or HEDJ/ERdj3 are provided, as well as uses thereof....

20050287665 - Method for inducing neural differentiation: The present invention provides a method for inducing neural differentiation comprising treating a bone marrow stem cell with a neurotrophic factor and/or dibutyryl cAMP (dbcAMP), wherein the neurotrophic factor comprises glial cell line-derived neurotrophic factor (GDNF) or pituitary adenylate cyclase-activating polypeptide (PACAP)....

20050287666 - Cell culture medium comprising transition metals or trace elements: The present invention provides improved cell culture media, media supplements, methods of manufacture of said media and methods of culturing cells. Transition metals or trace elements are controlled in a manner to provide improved conditions for culturing cells. Also provided are methods for supplementing media to improve culture and the...

20050287670 - Cell culturing systems, methods and apparatus: The present disclosure provides cell-culturing methods, apparatus and systems wherein cells in cell cultures are subjected to novel shear forces, which provide improved and efficient target product production. The shear force is provided by a reactor apparatus connected to a cell culture apparatus by a pump for transporting said cell...

20050287667 - Methods and compositions for the inhibition of gene expression: The present invention relates to methods and compositions for the inhibition of gene expression. In particular, the present invention provides oligonucleotide-based therapeutics for the inhibition of oncogenes involved in cancers....

20050287669 - Nucleic acid construct and expression vector for enhancing the production of recombinant protein, and method for the massive production of recombinant protein: Disclosed herein are nucleic acid constructs and expression vectors for enhancing the production of recombinant polypeptides/proteins, and methods for the massive production of recombinant polypeptides/proteins, in which a first nucleic acid sequence encoding thioredoxin and a second nucleic acid sequence encoding hemoglobin are cloned into a host cell, thereby enhancing...

20050287668 - Rna interference compositions and screening methods for the identification of novel genes and biological pathways: The present invention provides compositions and methods for enhancing RNA interference and facilitating the use of long RNA interference molecules. Accordingly, the invention includes a variety of novel applications of RNA interference, including methods related to screening RNA interference molecules using reporter genes to identify biological pathways, genes, therapeutic compounds...

20050287672 - Avipox recombinants expressing foot and mouth disease virus genes: The present invention relates to modified poxviral vectors and to methods of making and using the same. In particular, the invention relates to recombinant avipox that expresses gene products of foot and mouth disease virus (FMDV), and to compositions or vaccines that elicit immune responses directed to FMDV gene products...

20050287671 - Soluble steroidal peptides for nucleic acid delivery: Amphiphilic lipopeptide compositions for gene delivery are disclosed. An illustrative amphiphilic lipopeptide composition includes a human protamine 2 peptide conjugated to a hydrophobic moiety. Illustrative hydrophobic moieties include sterols, bile acids, and fatty acids. The amphiphilic lipopeptide composition is mixed with a nucleic acid such that the nucleic acid binds...

20050282144 - Method and system for removing solutes from a fluid using magnetically conditioned coagulation: A method for removing a solute from a fluid using magnetically conditioned coagulation includes magnetically conditioning the fluid by applying a conditioning magnetic field to enhance the precipitation of solute particles for coagulation; adding a coagulant to the fluid before, during, or after application of the conditioning magnetic field to...

20050282143 - Use of visible light at wavelengths of 500 nm and higher to pathogen reduce blood and blood components: This invention is directed towards a method of using visible light at wavelengths of at least 500 nm and above in combination with a sensitizer having a flavin moiety to reduce any pathogens that may be contained in blood and blood components. By exposing the blood and blood components to...

20050282150 - Antibodies to cyclin e2 protein: Disclosed are nucleic acid molecules encoding novel cyclin E2 polypeptides. Also disclosed are methods of preparing the nucleic acid molecules and polypeptides, and methods of using these molecules....

20050282148 - Artificial immune system: methods for making and use: The present invention relates to methods of constructing an integrated artificial immune system that comprises appropriate in vitro cellular and tissue constructs or their equivalents to mimic the normal tissues that interact with vaccines in mammals. The artificial immune system can be used to test the efficacy of vaccine candidates...

20050282149 - Closed-loop electrical stimulation system for cell cultures: The present invention provides a sensitive system for measuring the physiological response of an in-vitro cell culture to an environmental parameter. An electrical property of the cell culture is measured as a control signal, and a parameter of a stimulus is adjusted in real time to maintain the control signal...

20050282152 - Drug testing with bio-artificial organ slices including for example those derived from liver: A drug testing system using any number of bio-artificial organs, for example liver-slices based in a culture apparatus. The apparatus has a chamber with plasma and gas valves, animal organ slices being positioned securely in the chamber so as to maximize the surface area of organ slices exposed to the...

20050282151 - Method and apparatus for sorting particles with a mems device: A micromechanical particle sorting chip uses laser light directed through at least one of a reflective and refractive surface to come to a focus in an optically transparent layer. The laser light impinges on a particle of interest, causing it to fluoresce. Upon detecting the fluorescence, a micromemchanical actuator is...

20050282145 - Method for identifying anti-neoplastic compounds: Aspects of the present invention relate to methods of evaluating substances for their effects on cellular proliferation. Particular aspects of the invention use cultures of cells containing more than one type of cell....

20050282147 - Methods for assaying gene imprinting and methylated cpg islands: Genomic imprinting is a parent of origin-dependent gene silencing that involves marking of alleles in the germline and differential expression in somatic cells of the offspring. Imprinted genes and abnormal imprinting have been implicated in development, human disease, and embryonic stem cell transplantation. We have established a model system for...

20050282146 - Methods to predict additional nodal metastases in breast cancer patients: A method and system to predict the likelihood of additional nodal metastases in breast cancer patients with a positive sentinel node biopsy....

20050282154 - Angiotensin-converting enzyme-2 as a receptor for the sars coronavirus: The present invention is based upon the identification of human angiotensin-converting enzyme-2 (ACE-2) as a functional receptor for the SARS coronavirus. Transfection of cells with ACE-2 confers upon them the ability to support viral replication. In addition, assays performed using ACE-2 together with the S protein of the SARS virus...

20050282156 - Methods for making a device for concurrently processing multiple biological chip assays: Methods for concurrently processing multiple biological chip assays by providing a biological chip plate comprising a plurality of test wells, each test well having a biological chip having a molecular probe array; introducing samples into the test wells, subjecting the biological chip plate to manipulation by a fluid handling device...

20050282153 - Quantification of microorganism: A method for quantifying a microorganism and related primers and nucleic acids....

20050282155 - Viral libraries from uncultivated viruses and polypeptides produced therefrom: Provided is a method for producing viral genomic and complementary DNA expression libraries and novel viral polypeptides without requiring virus cultivation prior to library construction. The method includes direct isolation of viral particles from the environment by differential filtration and centrifugation. The viral nucleic acids are extracted and used to...

20050282189 - 2150, human protein kinase family member and uses therefor: The invention provides isolated nucleic acids molecules, designated 2150 nucleic acid molecules, which encode novel protein kinase family members. The invention also provides antisense nucleic acid molecules, recombinant expression vectors containing 2150 nucleic acid molecules, host cells into which the expression vectors have been introduced, and nonhuman transgenic animals in...

20050282226 - Aptamer capable of specifically adsorbing to bisphenol a and method for obtaining the aptamer: The present invention provides an aptamer capable of specifically adsorbing to bisphenol A suspected to be an endocrine disrupter as a target molecule, a method for obtaining an aptamer capable of specifically adsorbing to bisphenol A by an in vitro selection method utilizing affinity chromatography using a carrier immobilizing bisphenol...

20050282179 - Biomarkers for interferon-alpha response in hepatitis c virus infected patients: The present invention relates to the identification of prognostic markers useful in determining the response of hepatitis c virus (HCV) infected patients to interferon alpha (IFN-α) treatment. The studies provide biomarkers that can be used to discern sustained responders and non-responders of IFN-α treatment. This information should enable treating physicians...

20050282203 - Caged rnas and methods of use thereof: Methods of using labeled interfering RNAs to detect and/or quantitate target mRNAs in cells are provided. Related compositions, systems, and kits are also provided. Caged interfering RNAs (e.g., photoactivatable interfering RNAs), methods of using such caged RNAs, and related systems and kits are also provided. Caged RNAs capable of repressing...

20050282168 - Cell surface molecules as markers and therapeutic agents against kidney cancers: Human cell surface molecules CD70 and CD203c are expressed at higher levels in kidney carcinomas, particularly renal cell carcinomas and clear cell renal cell carcinomas, yet are expressed at low levels in normal kidney and other diseased kidney tissue, and at low levels in other tissues. CD70 and CD203c show...

20050282201 - Cell-based detection and differentiation of lung cancer: The present invention provides a method for detecting and differentiating disease states with high sensitivity and specificity. The method allows for a determination of whether a cell-based sample contains abnormal cells and, for certain diseases, is capable of determining the histologic type of disease present. The method detects changes in...

20050282208 - Cellular phenotype: Phenotypes and the cells that exhibit those phenotypes are described. The phenotype may be established as a “snapshot” of the cells at a particular time or it may be established as a variation in features over time, or as some combination of these “static” and “dynamic” characterizations. The phenotype may...

20050282195 - Compositions, methods, and kits for (mis)ligating oligonucleotides: Methods, reagents, and kits for (mis)ligating oligonucleotide probes or for identifying at least one target nucleotide are disclosed. One can enhance the generation of misligation product using a ligase under reaction conditions and with reagents where that particular ligase is prone to misligation. Alternatively, one can decrease or avoid generating...

20050282206 - Continous flow thermal device: The present invention provides a thermal cycling device including: a heat exchanger body (3) having a longitudinal axis and longitudinally divided to provide at least two segments which are able to be heated to different temperatures so that said body has peripheral surfaces of different temperatures; a conduit (2) extending...

20050282194 - Detection of shiga toxin- or shiga-like toxin-producing organisms: The invention provides methods to detect Shiga toxin- or Shiga-like toxin-producing organisms, particularly Shiga-like toxin-producing E. coli organisms, in biological samples using real-time PCR. Primers and probes for the detection of Shiga toxin- or Shiga-like toxin-producing organisms are provided by the invention. Articles of manufacture containing such primers and probes...

20050282165 - Diagnosis of carcinoma using raig1 polypeptides: The present invention relates to the new uses of a polypeptide (retinoic acid-inducible gene 1; RAIG1, also known as hypothetical protein FLJ10899 or retinoic acid induced 3) compositions comprising the polypeptide, including vaccines, antibodies that are immunospecific for the polypeptide and agents which interact with or modulate the expression or...

20050282157 - Diagnosis of diseases associated with dna replication: Chemically modified genomic sequences of genes associated with DNA replication, to oligonucleotides and/or PNA-oligomers for detecting the cytosine methylation state of genes associated with DNA replication which are directed against the sequence are disclosed. In addition, a method for ascertaining genetic and/or epigenetic parameters of genes associated with DNA replication...

20050282192 - Diagnosis, prognosis and identification of potential therapeutic targets of multiple myeloma based on gene expression profiling: Gene expression profiling is a powerful tool that has varied utility. It enables classification of multiple myeloma into subtypes and identifing genes directly involved in disease pathogensis and clinical manifestation. The present invention used gene expression profiling in large uniformly treated population of patients with myeloma to identify genes associated...

20050282198 - Diagnostics and therapeutics for diseases associated with an il-1 inflammatory haplotype: Methods and kits for determining whether a subject has or is predisposed to developing a disease which is associated with IL-1 polymorphisms and assays for identifying therapeutics for treating and/or preventing the development of these diseases are provided....

20050282186 - Fluorescence based biosensor: A novel biosensor comprises at least one fluorophore and at least two quenchers, and is capable of selectively and specifically detecting the presence of an ion in the presence of other ions....

20050282205 - Glut5 transporters expressed in cancer cells: GLUT5 is consistently expressed at high levels in cancer cells. Disclosed herein are assays for determining whether a test material/molecule is a substrate for, and/or is transported by, the GLUT5 transporter, and therefore a candidate substrate for transport into cancer cells. The assays are useful in screening for cytotoxic agents...

20050282214 - Human protease molecules: The invention provides human protease molecules (HUPM) and polynucleotides which identify and encode HUPM. The invention also provides expression vectors, host cells, antibodies, agonists, and antagonists. The invention also provides methods for treating or preventing disorders associated with expression of HUPM....

20050282217 - Human tumor necrosis factor receptor tr10: The present invention relates to a novel protein, TR10, which is a member of the tumor necrosis factor (TNF) receptor superfamily and the TRAIL receptor subfamily. In particular, isolated nucleic acid molecules are provided encoding the human TR10 protein. TR10 polypeptides are also provided as are vectors, host cells and...

20050282212 - Identification of the gene and mutation for progressive rod-cone degeneration in dog and a method for testing same: Tools and methods are provided for determining whether or not a dog is genetically normal, is a carrier of, or is affected with or predisposed to progressive rod-cone degeneration. The method is based on the detection of a transversion from G to A at position corresponding to nucleotide position 1298...

20050282175 - Image processing method and system for microfluidic devices: A method for processing an image of a microfluidic device. The method includes receiving a first image of a microfluidic device. The first image corresponds to a first state. Additionally, the method includes receiving a second image of the microfluidic device. The second image corresponds to a second state. Moreover,...

20050282197 - Interpreting dna: Methods for establishing the genotype of a DNA sample, and methods for investigating the potential sources of a DNA sample arising from a plurality of source, are provided, the methods being based on a method including: analysing the sample to produce a data profile for the sample for a locus;...

20050282215 - Isolated cytokine receptor licr-2: The invention relates to new members of the Class II cytokine receptor family, such as LICR-2. The new member binds to AK155, and mediates STAT activation....

20050282202 - Kits and processes for removing contaminants from nucleic acids in environmental and biological samples: The invention provides methods and compositions, e.g., kits, for removing contaminants from nucleic acids in a sample, e.g., environmental or biological samples such as soil, food, plant, animal, microorganism or water samples. The invention provides methods and compositions for isolating nucleic acids from environmental and biological samples in a scaleable...

20050282176 - Method for analyzing microbial communities: The present invention provides a method for quantitatively analyzing microbial genes, species, or strains in a sample that contains at least two species or strains of microorganisms. The method involves using an isothermal DNA polymerase to randomly and representatively amplify genomic DNA of the microorganisms in the sample, hybridizing the...

20050282224 - Method for carrying out a biochemical protocol in continuous flow in a microreactor: Devices and methods for carrying out a chemical or biochemical protocol are disclosed. In one embodiment, the chemical or biochemical protocol is performed by cycling at least one thermal transfer member between at least two temperatures while liquid samples on which the chemical or biochemical protocol is to be performed...

20050282166 - Method for the expression of unknown environmental dna into adapted host cells: The present invention relates to methods for the identification or cloning of polynucleotides encoding a selected phenotype, particularly from environmental DNA. In a specific embodiment, the method comprises (i) cloning environmental DNA fragments into E coli cloning vectors to produce a metagenomic library, (ii) identifying or selecting cloning vectors in...

20050282207 - Method of stress evaluation: This invention relates to a simple method for evaluating stress of a normal healthy subject with high accuracy is provided. In this method, the expression profile of specific genes that serve as stress marker genes is analyzed, and stress of the subject is evaluated based on the analysis results....

20050282170 - Method to detect prostate cancer in a sample: The present invention relates to prostate cancer. More specifically, the present invention relates to a method to detect prostate cancer in a patient sample by detecting the RNA encoded by the gene PCA3. More particularly the present invention relates to a method for determining a predisposition, or presence of prostate...

20050282199 - Method to predict prostate cancer: A method for predicting the probability or risk of prostate cancer is provided....

20050282196 - Methods and compositions for cancer diagnosis: This invention relates generally to the field of cancer diagnostics. The invention further relates to the use of mutational load distribution analysis (MLDA) to examine changes in the distribution of genetic mutations incident to cancer....

20050282191 - Methods and compositions for evaluation of fertility: The present invention concerns compositions and methods for evaluating fertility in humans and animals. The invention may also be used to identify reproductive cancers such as testicular cancer. In various embodiments of the invention, an Sptrx-3 enzyme is used as a fertility marker. Sptrx-3 may be detected in accordance with...

20050282183 - Methods and compositions for pearl oyster cultivation: The invention provides methods and compositions useful in pearl oyster cultivation. Polynucleotide and polypeptides relating to the nacre gene of Pinctada margaritifera are provided. Antibodies related to these polypeptides, and compositions comprising polynucleotides, polypeptides and/or antibodies of the invention are also provided. The invention provides methods of using these polynucleotides,...

20050282167 - Methods and compositions for regulating adipogenesis: According to the disclosure hypoxia-mediated adipogenic inhibition involves the repression of PPARγ2 expression and its activity is a common mechanism for adipogenic inhibition by a variety of stimuli. The present disclosure relates to methods and compositions for regulating adipogenesis. The disclosure provides compositions comprising one or more DEC1/Stra13 fragments of...

20050282200 - Methods and devices related to a regulated flow electroporation chamber: The electroporation chamber and its related devices combine the features of an electroporation chamber that acts as a manifold for regulation of sample flow with those of a flow electroporation device to form a regulated flow electroporation device. The invention further comprises a novel regulated flow electroporation chamber that enables...

20050282213 - Methods and kits useful for detecting an alteration in a locus copy number: A method of identifying an alteration in a locus copy number is provided. The method is effected by determining a methylation state of at least one gene in the locus, wherein a methylation state differing from a predetermined methylation state of the at least one gene is indicative of an...

20050282184 - Methods and reagents for molecular cloning: The present invention provides compositions, methods, and kits for covalently linking nucleic acid molecules. The methods include a strand invasion step, and the compositions and kits are useful for performing such methods. For example, a method of covalently linking double stranded (ds) nucleic acid molecules can include contacting a first...

20050282174 - Methods and systems for selecting nucleic acid probes for microarrays: Methods and systems for identifying and selecting nucleic acid probes for detecting a target with a nucleic acid probe array or microarray, comprising selecting a plurality of candidate probes, forming a plurality of clusters from the plurality of candidate probes according to hybridization characteristics of the candidate probes, forming at...

20050282185 - Methods for detecting dna originating from different individuals: In a first aspect, the present invention features methods for differentiating DNA species originating from different individuals in a biological sample. These methods may be used to differentiate or detect fetal DNA in a maternal sample or to differentiate DNA of an organ donor from DNA of an organ recipient....

20050282181 - Methods for identifying functional antibodies: The invention provides methods for screening multimeric antibodies produced by mammalian cells to find those that exhibit a biological function. The methods can be used to screen large numbers of antibodies, which may be cell surface, secreted, or intracellular antibodies. Antibodies can be screened to find those that bind antigen...

20050282162 - Methods of validating snps and compiling libraries of assays: Libraries of assays and methods of compiling the libraries are provided. The assays can identify Single Nucleotide Polymorphisms (SNPs). Methods of validating SNPs are provided. Methods of constructing linkage disequilibrium maps using sets or subsets of SNPs are also provided....

20050282204 - Microarray substrate having patterned thin layer and microarray comprising the same, and methods of producing the microarray substrate and the microarray: A microarray substrate having a patterned thin layer on a substrate is provided. In the microarray substrate, the substrate has a reflectance different from that of a material of the thin layer, and the patterned thin layer includes a spot region having a thickness at which constructive interference occurs between...

20050282218 - Microparticle-based methods and systems and applications thereof: Microparticle-based analytical methods, systems and applications are provided. Specifically, the use of resonant resonant light scattering as an analytical method for determining either or both a particle's identity and the presence and optionally, the concentration of one or more particular target analytes is described. Applications of these microparticle-based methods in...

20050282219 - Microparticle-based methods and systems and applications thereof: Microparticle-based analytical methods, systems and applications are provided. Specifically, the use of resonant resonant light scattering as an analytical method for determining either or both a particle's identity and the presence and optionally, the concentration of one or more particular target analytes is described. Applications of these microparticle-based methods in...

20050282220 - Microparticle-based methods and systems and applications thereof: Microparticle-based analytical methods, systems and applications are provided. Specifically, the use of resonant resonant light scattering as an analytical method for determining either or both a particle's identity and the presence and optionally, the concentration of one or more particular target analytes is described. Applications of these microparticle-based methods in...

20050282190 - Modular design and construction of nucleic acid molecules, aptamer-derived nucleic acid constructs, rna scaffolds, their expression, and methods of use: The present invention relates to a nucleic acid molecule comprised of first and second nucleic acid elements that each bind a target molecule, and a three-way junction operably linking the first and second nucleic elements. Also disclosed is an RNA scaffold comprising first and second RNA receptor regions operably linked...

20050282222 - Nematode pan and zp receptor-like sequences: Nucleic acid molecules from nematodes encoding PAN and ZP domain containing receptor polypeptides are described. PANZP polypeptide sequences are also provided, as are vectors, host cells, and recombinant methods for production of PANZP nucleotides and polypeptides. Also described are screening methods for identifying inhibitors and/or activators, as well as methods...

20050282178 - Novel human gene with immunoregulatory and anti-proliferative properties: This invention describes the human gene homologue of the mouse gene previously identified. The human gene is located on the long arm of chromosome 17. The human gene, termed G1h, encodes for a protein, termed P1h, that substantially differs from the mouse protein. The human protein P1h can be used...

20050282161 - Novel phospholipase a2 and gene thereof: To provide a novel phospholipase A2 (PLA2) associated with psoriasis, a nucleic acid encoding the same, a method for characterizing, identifying or screening an inhibitor for the PLA2 or a medicament; and a novel method for diagnosis or examination of psoriasis or the like. A polypeptide having the amino acid...

20050282163 - Polymorphisms in elastin, fibrillin and related genes as predisposing to restenosis and to a therosclerosis: Methods are provided for assessing the risk of developing restenosis in an individual, by detecting the presence of biologically important polymorphisms in genes involved in the formation of the elastin fiber network. In particular, detection of polymorphorphisms in the elastin, fibrillin, fibrillin-1, fibrillin-2, lysyl oxidase, microfibril-associated glycoprotein, biglycan, osteopontin, and/or...

20050282211 - Probe optimization methods: The present invention provides methods for optimizing nucleic acid detection assays for use in basic research and clinical research. Specifically, the invention provides a method for empirically optimizing nucleic acid probes by testing them with samples containing genomic DNA with variations in copy number in different regions of the genome....

20050282221 - Process for manufacturing a microfluidic device with buried channels: A process for manufacturing a microfluidic device, including the steps of: forming at least one channel in a semiconductor material body; forming a dielectric diaphragm above the channel, for closing the channel; and forming heating elements for providing thermal energy inside the channel. The heating elements are formed directly on...

20050282216 - Purified polypeptides having il-13 receptor activity: This invention relates to a purified polypeptide, comprising an amino acid sequence chosen from: a) the sequence SEQ ID No. 2, b) any biologically active sequence derived from SEQ ID No. 2....

20050282171 - Purified thermostable pyrococcus furiosus dna polymerase i: Purified thermostable Pyrococcus furiosus DNA polymerase that migrates on a non-denaturing polyacrylamide gel faster than phosphorylase B and Taq polymerase and more slowly than bovine serum albumin and has an estimated molecular weight of 90,000-93,000 daltons when compared with a Taq polymerase standard assigned a molecular weight of 94,000 daltons....

20050282225 - Quantitative binding assays using green fluorescent protein as a label: A heterogeneous binding assay for an analyte in a fluid sample is developed, which uses a green fluorescent protein (GFP) label. A ligand-GFP conjugate has a specific binding affinity for an anti-ligand immobilized on a support. The anti-ligand also has a specific binding affinity for the analyte. Competition between the...

20050282182 - Reaction vessel and reaction apparatus comprising three-dimensional particle array: It is an object of the present invention to provide a reaction vessel comprising a three-dimensional particle array, with which reaction efficiency is increased by raising the probability of encounter between the target substance in the liquid sample and the reactive substance immobilized on the particle surface, and a reaction...

20050282187 - Reagents and methods useful for detecting diseases of the urinary tract: A set of contiguous and partially overlapping cDNA sequences and polypeptides encoded thereby, designated as UT116 and transcribed from urinary tract tissue, is described. These sequences are useful for the detecting, diagnosing, staging, monitoring, prognosticating, in vivo imaging, preventing or treating, or determining the predisposition of an individual to diseases...

20050282188 - Rna interference mediated inhibition of gene expression using short interfering nucleic acid (sina): This invention relates to compounds, compositions, and methods useful for modulating gene expression using short interfering nucleic acid (siNA) molecules. In particular, the instant invention features small nucleic acid molecules, such as short interfering nucleic acid (siNA), short interfering RNA (siRNA), double-stranded RNA (dsRNA), micro-RNA (miRNA), and short hairpin RNA...

20050282158 - Rolling circle replication of padlock probes: Rolling circle replication of a padlock primer is inhibited when it is hybridised to a target nucleic acid that is long or circular. The invention provides methods of addressing this problem including cutting the target nucleic acid near or preferably at the site which hybridises with the padlock probe, whereby...

20050282159 - Rpob gene of streptomyces, primer specific to streptomyces, and identification method of streptomyces having rifampin resistance or sensitivity by using the same: The present invention relates to a polynucleotide having a 306-bp fragment of an ANA polymerase gene subunit B (rpoB) of Streptomyces, and an identifying method of Streptomyces species using the same. According to the identifying method, the Streptomyces can be detected or identified accurately, economically, and easily. In addition, the...

20050282169 - Signatory sequences: The invention includes a method of labeling a biological polymer involving including within the polymer a series of monomers that encode a source of origin or other useful information regarding the biological polymer. Preferably, the biological polymer is DNA, and the series of monomers spell the name of the entity...

20050282173 - Single molecule fluorescence assay: An assay method for acquiring sequence information about a single nucleic acid molecule is provided. Probes and kits are also provided for practicing the method of the invention....

20050282160 - Sodium-independent transporter transporting small-sized neutral amino acid, gene thereof and method of analyzing transporter function by constructing fused proteins enabling the specification of the function: It is intended to provide a sodium-independent transporter which transports a small-sized neutral amino acid; its gene; fused proteins which enable the specification of the function thereof; and a method of analyzing the function of the transporter using the same. A protein having an amino acid sequence represented by SEQ...

20050282180 - Solid medium and method for dna storage: A solid medium for storage of DNA, including blood DNA, comprising a solid matrix having a compound or composition which protects against degradation of DNA incorporated into or absorbed on the matrix. Methods for storage of DNA using this solid medium, and for recovery of DNA or in situ use...

20050282193 - Space efficient polymer sets: The disclosure features a collection that comprises a plurality of polymers, typically nucleic acid molecules in a compact form. The molecules include all possible sequences or at least a certain percentage of all possible sequences, of a particular length....

20050282172 - System and method for antigen structure-independent detection of antigens captured on antibody arrays: The present invention provides a system and method for detecting antigens captured on an antibody array. The method comprises the following steps of providing the antibody array having at least two antibodies, contacting the antibody array with a sample containing at least one antigen that may be captured by the...

20050282223 - Tr3-specific binding agents and methods for their use: Biologically active TR3-specific binding agents and methods for their use are disclosed. The biologically active TR3-specific binding agents are useful for inhibiting the proliferation of cells expressing TR3. These biologically active agents are particularly useful for treating T-cell mediated diseases such as graft-versus-host disease, organ rejection, tumor growth, autoimmunity, and...

20050282227 - Treatment discovery based on cgh analysis: Methods, systems and computer readable media for discovering a combination of treatments to reduce the progress of, or eliminate a tissue malady. Gene expression values of at least one sample of tissue exhibiting the tissue malady and at least one reference sample tissue that does not exhibit the malady are...

20050282177 - Types of lymphoma and method for prognosis thereof: A method for determining the prognosis of a CD5+DLBCL patient and a CD5−DLBCL patient is provided. It is determined that, in the chromosomal DNA from a patient with lymphoma, (1) the prognosis of the CD5+DLBCL patient with amplification of 13q21.1-q31.3 region is poor; (2) the prognosis of the CD5+DLBCL patient...

20050282164 - Ultraphobic sample carrier having functional hydrophilic and/or oleophilic areas: The invention relates to a planar structure having an ultraphobic surface and at least one hydrophilic and/or oleophilic area that, in addition to being hydrophilic and/or oleophilic, has at least one additional functionality. The invention also relates to a method for producing the inventive planar structure and to the use...

20050282210 - Vaccination of skunks and/or mongooses against rabies: The present invention relates to recombinant anti-rabies vaccines and the oral administration of such vaccines to skunks and/or mongooses. Advantageously, the anti-rabies vaccine may comprise a recombinant vaccinia virus containing a rabies glycoprotein gene. The invention encompasses methods of vaccinating skunks and/or mongooses by administration of an anti-rabies vaccines which...

20050282209 - Variable length probe selection: The present invention provides novel method for increasing the efficiency and accuracy of high-throughput mutation mapping and genome resequencing by using a variable length probe selection algorithm to rationally select probes used in designing oligonucleotide arrays synthesized by Maskless Array Synthesis (MAS) technology. Also disclosed is a variable length probe...

20050282230 - Method for making monoclonal antibodies and cross-reactive antibodies obtainable by the method: A method of making monoclonal antibodies according to a mixed antigen immunization protocol is described. In addition, antibodies obtainable by the method are disclosed which specifically cross-react with two or more different receptors to which Apo-2 ligand (Apo-2L) can bind....

20050282228 - Methods and compositions for activating or inhibiting vegf-d and vegf-c: Methods for activating endothelial growth factors VEGF-C or VEGF-D with plasmin, and methods of treatment comprising administering a phamarceutical compositions comprising plasmin. Also disclosed are methods for screening for inhibitors of activation of the growth factors by plasmin, and method of treatment by blocking activation of VEGF-C/D activation by plasmin....

20050282229 - Methods and device for analyte characterization: The methods and apparatus, disclosed herein are of use for sequencing and/or identifying proteins, polypeptides and/or peptides. Proteins containing labeled amino acid residues may be synthesized and passed through nanopores. A detector operably coupled to a nanopore may detect labeled amino acid residues as they pass through the nanopore. Distance...

20050282231 - Screening assays for hedgehog agonists and antagonists: The present invention concerns the discovery that proteins encoded by a family of vertebrate genes, termed here hedgehog-related genes, comprise morphogenic signals produced by embryonic patterning centers, and are involved in the formation of ordered spatial arrangements of differentiated tissues in vertebrates. The present invention makes available compositions and methods...

20050282232 - Compositions and methods for modulating c-rel-dependent cytokine production: The present invention is directed to compositions and methods for modulating c-Rel-dependent cytokine production without materially altering the level of expression of NFκB and/or the amount of IκB. The present invention is also directed to screening for modulators of c-Rel activity as determined by assaying for altered subcellular localization of...

20050282233 - Multivalent antibody materials and methods for vegf/pdgf family of growth factors: The present invention relates to materials and methods for modulating angiogenesis. The compositions of the invention provide antibody substances specific for two or more PDGF/VEGF family members, which are useful for modulating angiogenesis and lymphangiogenesis in a subject....

20050282234 - Real time method of detecting acute inflammatory conditions: This invention relates to the diagnosis and/or monitoring of patients with inflammatory diseases or conditions, including systemic lupus erythematosus, particularly for diagnosis of the acute stage of the disease, including methods and kits for carrying out this activity. This disclosure presents the surprising discovery that levels of complement pathway components...

20050282235 - Process and device for producing reagent carriers: The invention concerns a process for producing reagent carriers having a binding capability that are suitable for determining analytes in liquids, wherein the process comprises at least one step of treating a respective reagent carrier body in particular by transferring material between a treatment device and the reagent carrier body...

20050282236 - Inhibitors of macrophage migration inhibitory factor and methods for identifying the same: e

20050282237 - Immunological analysis carrier and an immunological analysis method using the same: The present invention provides an immunological analysis carrier comprising at least one of an antibody against a target substance, an agent capable of specifically binding a target substance, a part of an antibody against a target substance and a part of an agent capable of specifically binding a target substance,...

20050282238 - High-sensitivity chemiluminescent elisa prion detection method: A high-sensitivity chemiluminescent ELISA-based prion detection method is provided, the method comprising an ELISA plate coated with monoclonal antibody for the capture of PrPsc protein and treated with an optimized blocking reagent, and a set of labeling and detection reagents. The digestion cocktail is preferably a unique combination of proteolytic,...

20050282239 - Cell-permeable enzyme activation reporter that can be loaded in a high throughput and gentle manner: A membrane traversing peptide conjugate comprises (i) a label, (ii) a target peptide, attached to the label, and (iii) a transduction domain, attached to the target peptide. The target peptide is a reactant for a chemical reaction occurring in a cell....

20050282240 - Method for identifying peptides in a biological sample: The invention provides a method for isolating a cargo peptide from a sample containing a cargo peptide-carrier protein complex. The method involves contacting a sample comprising a cargo peptide-carrier protein complex with a binding moiety selective for the carrier protein, under conditions wherein the carrier protein binds non-covalently to the...

20050282241 - Method of scavenging intermediate formed by reaction of oxidoreductase with substrate: The present invention is the method to trap reliably the reaction intermediates of an oxidoreductase, such as cytochrome-c oxidase. The present invention is a method for trapping reaction intermediates of an oxidoreductase comprising the steps of: (the first step) dissolving an oxidoreductase, a photoinduced reducing agent that releases electrons by...

20050282242 - Screening assays for antimicrobial agents: The present invention provides methods for screening antimicrobial agents that make use of mutated microbial polypeptides. Because these polypeptides have biological activity and result in increased antimicrobial drug resistance, candidate compounds that specifically target these polypeptides provide therapeutics or therapeutic lead compounds for treating infections caused by drug resistant microbial...

20050282243 - Device for susceptibility testing of bacterial pathogens and vaccine strains in poultry: Device for susceptibility testing of bacterial pathogens and vaccine strains in poultry with a microtiter plate comprising a plurality of wells and at least one well with a coating containing an antibiotic of a marker of a vaccine strain for the vaccination of poultry....

20050282244 - Method and apparatus for viable and nonviable prokaryotic and eukaryotic cell quantitation: A rapid method for the quantitation of various live cell types is described. The method may include a variety of steps including: 1) suspending the cells in a detergent-like compound, 2) isolating the washed cells by centrifugation or filtration, 3) resuspending the cells in a solution that contains a preservative,...

20050282245 - Use of a composition which regulates oxidation/reduction reactions intracellularly and/or extracellularly in a staining or sorting process: Staining mixtures comprising viable spermatozoa, a composition which regulates oxidation/reduction reactions intracellularly or extracellularly, and a DNA selective dye are disclosed. The cells contained in such suspensions tend to have a greater capacity for enduring the various process steps typically associated with the sorting of sperm cells into gender enriched...

20050282246 - Frozen tissue microarray: A tissue microarray includes frozen tissue cores that extend from the top of the tissue microarray to a release. The release is a material from which embedding material of the tissue microarray can easily be removed. A stiffener is used with the release to maintain a flat shape of the...

20050282256 - Adipocyte complement related protein zacrp13: The present invention relates to polynucleotide and polypeptide molecules for zacrp13, a novel member of the family of proteins bearing a collagen-like domain and a C1q domain. Novel zacrp13 polypeptides, polynucleotides encoding the polypeptides, and related compositions and methods are disclosed. Also disclosed are antibodies to the zacrp13 protein or...

20050282248 - Chemically modified mutant serine hydrolases show improved catalytic activity and chiral selectivity: This invention provides novel chemically modified mutant serine hydrolases that catalyze a transamidation and/or a transpeptidation and/or a transesterification reaction. The modified serine hydrolases have one or more amino acid residues in a subsite replaced with a cysteine, wherein the cysteine is modified by replacing the thiol hydrogen in the...

20050282251 - Heparanase ii, a novel human heparanase paralog: The present invention provides a cDNA encoding a heretofore unknown enzyme termed heparanase II; constructs and recombinant host cells incorporating the cDNA; the heparanase II polypeptide encoded by the gene; antibodies to the polypeptide; and methods of making and using all of the foregoing....

20050282250 - Method for the recombinant production and purification of protein kinases: A method for the recombinant production and purification of a protein kinase selected from the group consisting of tyrosine protein kinases and serine/threonine kinases by expressing a nucleic acid encoding said kinase in a microbial host cell, forming inclusion bodies containing said kinase, isolating, solubilizing, naturing, and purifying said kinase...

20050282255 - Monomeric protein of the tgf-beta family: The present invention is concerned with proteins selected from the members of the TGF-β superfamily, which are monomeric due to substitution or deletion of a cysteine which is responsible for dimer formation. The invention is also concerned with nucleic acids, encoding such monomeric proteins, vectors or host cells containing the...

20050282254 - Optimized recognition site of the alphavirus non-structural protease for tag removal and specific processing of recombinant proteins: The present invention discloses highly efficient novel recognition sites for Pro39 protease. The invention further provides a wide range of conditions for protein purification and modification with the novel recognition sites. The invention even further provides expression vectors for expression of fusion proteins in cells and method to purify fusion...

20050282249 - Recombinant hexose oxidase, a method of producing same and use of such enzyme: A method of producing hexose oxidase by recombinant DNA technology, recombinant hexose oxidase and the use of such enzyme, in particular in the manufacturing of food products such as doughs and dairy products, animal feed, pharmaceuticals, cosmetics, dental care products and in the manufacturing of lactones. Suitable sources of DNA...

20050282247 - Recombinant single chain antibody in heterotrophic algae for pathogen control and use: Expression of functionally active recombinant single chain antibody in prokaryotic and eukaryotic heterotrophic algae cells for pathogen treatment or control in aquaculture and agriculture applications is disclosed; and subsequent modification of the strains to generate transgenic algae propagated in defined conditions....

20050282252 - Rh(d)-binding proteins and magnetically activated cell sorting method for production thereof: The invention includes Rh(D) binding proteins, including antibodies, and DNA encoding such proteins. Methods of generating such proteins and DNAs are also included....

20050282253 - Therapeutic antiangiogenic endostatin compositions: Endostatin compositions capable of inhibiting endothelial cell proliferation, inhibiting angiogenesis and causing tumor regression are described. Specifically, amino acid sequences of endostatin proteins and nucleic acid sequences coding for endostatin proteins are provided....

20050282257 - Application of glucose transport mutants for production of aromatic pathway compounds: This invention describes methods for enhancing carbon flow into a pathway of a host cell to enhance the biosynthetic production of compounds therefrom, the host cells being selected based on being phenotypically Pts−/glucose+. Such host cells are capable of transporting glucose without consuming PEP, resulting in conservation of PEP which...

20050282258 - Microbial process for arginine production: The present invention relates to process for increased yield of the amino acid arginine from bacterial cultures by employing strains that have been genetically manipulated for both increased arginine biosynthesis and increased level of the Escherichia coli protein YggA or a protein that is substantially similar to Escherichia coli YggA....

20050282259 - Nucleotide sequences for encoding of the lysr2-gene: Isolated polynucleotide comprising a polynucleotide sequence chosen from the group consisting of a) polynucleotide which is identical to the extent of at least 70% to a polynucleotide which codes for a polypeptide which comprises the amino acid sequence of SEQ ID No. 2, b) polynucleotide which codes for a polypeptide...

20050282260 - Polypeptides and biosynthetic pathways for the production of monatin and its precursors: Methods and compositions that can be used to make monatin from glucose, tryptophan, indole-3-lactic acid, indole-3-pyruvate, and 2-hydroxy 2-(indol-3-ylmethyl)-4-keto glutaric acid, are provided. Methods are also disclosed for producing the indole-3-pyruvate and 2-hydroxy 2-(indol-3-ylmethyl)-4-keto glutaric acid intermediates. Compositions provided include nucleic acid molecules, polypeptides, chemical structures, and cells. Methods include...

20050282261 - Novel choline oxidases: The invention relates to novel choline oxidases, methods for obtaining the same, bodycare products, haircare products, shampoos, oral, dental and dental prosthesis care products, cosmetics, washing products, cleaning products, rinsing products, hand soaps, washing-up detergents and dishwasher detergents comprising novel choline oxidases and uses of the novel choline oxidases....

20050282262 - Substantially pure reverse transcriptases and methods of prooduction thereof: The present invention provides substantially pure reverse transcriptases, which are preferably substantially free from contamination with nucleic acids. The invention also provides methods for the production of these enzymes, and kits comprising these enzymes which may be used in synthesizing, amplifying or sequencing nucleic acid molecules, including through the use...

20050282263 - Flexible vaccine assembly and vaccine delivery platform: Herein described are various methods for making a vaccine that are made of re-assembled virus like particles (VLP). First, the VLPs are disassembled into coat proteins or encapsidation intermediate populations. Each population undergoes, for instance, chemical conjugation of unique peptide or nucleic moieties to form separate populations. Thereafter, a predetermined...

20050282264 - Apparatus for encapsulating cells: An apparatus for encapsulating cells is disclosed. According to one embodiment, the apparatus includes an indirect-pumping dispenser for dispensing a cell suspension into an encapsulation solution through an outlet of the dispenser. The apparatus also includes a dipping mechanism that is attachable to the outlet of the dispenser. The dipping...

20050282265 - Electroporation apparatus and methods: This document discloses electroporation vessels, electrocompetent cells that have been aliquoted and frozen in electroporation vessels, and a number of other apparatuses, kits, and methods for electroporation. Some embodiments of electroporation vessels described herein may include a pair of opposing walls that are downwardly angled toward one another in a...

20050282266 - Polymerase chain reactor: A polymerase chain reactor, comprises a temperature measuring system, a temperature controlling system and a human machine operational interface, whereby, the temperature in the course of the polymerase chain reaction detected by a temperature sensor disposed in a reaction tube is converted into an electric signal via analog/digital conversion, this...

20050282267 - Micro device and method for high resolusion delivery and monitoring of stimuli to a biological object, in vitro: A micro device and method are provided for examining and testing a slice of a biological object, such as brain tissue. The micro device includes a body defining a chamber and a channel in communication with the chamber. A stimulation fluid flows axially along a flow path in the channel...

20050282268 - Culture microscope and computer program controlling culture microscope: A culture microscope has an incubator chamber which controls a culture environment in which cells are cultured, an imaging optical system which photographs the cells, and a controller which controls a time-lapse photographing performed by the imaging optical system....

20050282269 - Disposable bioreactor/fermenter: The present invention is a bioreactor or fermenter formed of a container having two or more walled. One or more inlets and one or more outlets are formed in one or more of the walls of the container. At least one of the one or more inlets is formed in...

20050282270 - System for thermally cycling biological samples with heated lid and pneumatic actuator: Systems and methods for thermal cycling samples that include pneumatic automation are provided by the present teachings....

20050282271 - Glycoconjugates and methods: Methods for making the functionalized glycoconjugates include (a) contacting a cell with a first monosaccharide, and (b) incubating the cell under conditions whereby the cell (i) internalizes the first monosaccharide, (ii) biochemically processes the first monosaccharide into a second saccharide, (iii) conjugates the saccharide to a carrier to form a...

20050282275 - Adipose-derived stem cells and lattices: The present invention provides adipose-derived stem cells and lattices. In one aspect, the present invention provides a lipo-derived stem cell substantially free of adipocytes and red blood cells and clonal populations of connective tissue stem cells. The invention also provides a method of isolating stem cells from adipose tissues. The...

20050282273 - Medium and methods for culturing of avian primordial germ cells: The invention provides a novel culture medium useful for the proliferation and maintenance of avian primordial germ cells (PGCs) and encompassing a medium base, leukemia inhibitory factor, basic fibroblast growth factor, stem cell factor and insulin-like growth factor, and an avian serum. The invention also provides a method for the...

20050282274 - Osteoblast precursors from human embryonic stem cells: This invention provides populations of mesenchymal cells obtained from pluripotent stem cells by differentiating them ex vivo. Multipotent mesenchymal cells can in turn be differentiated into more specialized cell types such as osteoblasts, with properties that make them suitable for reconstituting musculoskeletal cell function in an individual. The compositions, methods,...

20050282272 - Using undifferentiated embryonic stem cells to control the immune system: This disclosure provides a system for minimizing the alloreactivity of tissue transplants. The patient is administered with undifferentiated embryonic stem cells or early progenitor cells. This induces a state of inflammatory quiescence or immune unresponsiveness, which in turn enhances engraftment of cells derived from the same stem cell line given...

20050282276 - Cell fractions containing cells capable of differentiating into neural cells: Demyelinated axons were remyelinated in the demyelinated rat model by collecting bone marrow cells from mouse bone marrow and transplanting the mononuclear cell fraction separated from these bone marrow cells....

20050282277 - Methods of increasing platelet and hematopoietic stem cell production: A method of increasing hematopoietic stem cell production is disclosed. The method includes administering a TPO mimetic compound to a subject. Pharmaceutical compositions including a TPO mimetic compound and a pharmaceutically acceptable carrier are also disclosed....

20050282278 - Plant sugar transport proteins: This invention relates to an isolated nucleic acid fragment encoding a sugar transport protein. The invention also relates to the construction of a chimeric gene encoding all or a portion of the sugar transport protein, in sense or antisense orientation, wherein expression of the chimeric gene results in production of...

20050282279 - Expression vector encoding coronavirus-like particle: The present invention relates to a expression vector for cloning the Class I viral fusion protein gene and as DNA vaccine candidate against virus infection diseases....

20050282282 - Methods of use of viral vectors to deliver antigen to dendritic cells: This invention relates to methods and compositions useful for delivering antigens to dendritic cells which are then useful for inducing T antigen specific cytotoxic T lymphocytes. This invention also provides assays for evaluating the activity of cytotoxic T lymphocytes. According to the invention, antigens are provided to dendritic cells using...

20050282280 - Oncolytic adenoviral vectors encoding gm-csf: Selectively replicating oncolytic adenoviral vectors comprising an adenoviral packaging signal a termination signal sequence, an E2F responsive promoter operably linked to an adenoviral coding region, a heterologous coding sequence encoding GM-CSF and a right ITR are provided. The oncolytic adenoviral vectors are useful for expressing GM-CSF in transduced cells and...

20050282281 - Recombinant baculoviral vector containing protein transduction domain gene: A recombinant baculoviral vector, which comprises a vesicular stomatitis virus glycoprotein (VSVG) gene and a protein transduction domain (PTD) gene derived from HIV-1 (human immunodeficiency virus-1) Tat gene, is useful in a gene therapy owing to its excellent efficiencies in the delivery of a target gene to the host cell...

20050282284 - Controlled electroporation and mass transfer across cell membranes in tissue: Electroporation is performed in a controlled manner in individual and multiple biological cells present in biological tissue by monitoring the electrical impedance, defined herein as the ratio of current to voltage in the electroporation cell. The impedance detects the onset of electroporation in the biological cell(s), and this information is...

20050282283 - Electroporation apparatus and methods: This document discloses electroporation vessels, electrocompetent cells that have been aliquoted and frozen in electroporation vessels, and a number of other apparatuses, kits, and methods for electroporation. Some embodiments of electroporation vessels described herein may include a pair of opposing walls that are downwardly angled toward one another in a...

20050277106 - Methods and active substances for protecting organs: The present invention comprises a method of protecting organs or tissue susceptible to reperfusion-induced dysfunction after ischemia. The method comprises parenterally administering to a patient a therapeutical composition containing natural alpha-1 acid glycoprotein, natural alpha-1 antitrypsin or a mixture thereof. Alternatively, organ or tissue transplants can be contacted with natural...

20050277108 - Method for extending the useful shelf-life of refrigerated red blood cells by nutrient supplementation: There are provided methods and compositions for the storage of red blood cells. The compositions are metabolic supplements that are preferably added to refrigerated red blood cells suspended in an additive solution. Red blood cells are preferably stored under conditions of oxygen-depletion. Metabolic compositions comprises pyruvate, inosine, adenine, and optionally...

20050277107 - Systems and methods for cell preservation: The present invention generally relates to devices and methods for the preservation of cells using drying, freezing, and other related techniques. In one set of embodiments, the invention allows for the preservation of cells in a dried state. In another set of embodiments, the invention allows for the preservation of...

20050277111 - Measuring method using surface acoustic wave device, and surface acoustic wave device and biosensor device: A measuring method using a surface acoustic wave device, with which even in a case where a target substance having a different viscosity is added to a buffer liquid on the surface acoustic wave device it is possible to measure a mass load accurately without suffering an effect of this...

20050277110 - Method of measuring the activity of g(alpha)i- or g(alpha)o-coupled receptors using ca2+ influx in cells: A method of measuring the activation or deactivation of G(alpha)i- or G(alpha)o-coupled receptors, and methods of identifying agonists or antagonists of such receptors....

20050277109 - Predictive assay for immune response: The present invention relates to an in vitro method for determining the ability of a vaccine composition which comprises one or more antigens or a nucleic acid molecule which encodes one or more antigens to stimulate a T cell response. In one embodiment, the method comprises the steps of: (1)...

20050277112 - Novel therapeutic processes for treating infectious agents using same epitope specific antigens, and useful compositions therefor: This invention provides novel processes for therapeutic applications, including the treatment of subjects carrying infectious agents or having impaired autoimmunity or impaired immune condition. The therapeutic applications disclosed herein are also directed at the treatment of cancerous subjects with malignant tumors containing cancerous cells or malignant or cancerous cells. Vaccination...

20050277113 - Saliva immunoassay for detection of exposure to infectious agents: A method for diagnosing the exposure to infectious agents in a patient is disclosed. The method determines the levels of antibodies against infectious agents, including bacterial, parasitic, and viral agents. It then compares the results to normal levels to determine the exposure to infectious agents....

20050277114 - System and method for detecting west nile virus: The present invention relates to a system for detecting West Nile Virus (WNV) in a sample by detecting nucleic acids having been amplified and comprising the coding region of the membrane protein of WNV. Further, a method and a kit for the detection of amplified nucleic acids comprising the coding...

20050277129 - Apoe genetic markers associated with age of onset of alzheimer's disease: Haplotypes in the APOE gene associated with age of onset of Alzheimer's Disease are disclosed. Compositions and methods for detecting and using these APOE haplotypes in a variety of clinical applications are disclosed. Such applications include articles of manufacture comprising compounds effective in delaying the age of onset of AD...

20050277145 - Apparatus with microtiter plate format for multiplexed arraying: The present invention relates generally to an apparatus and kit in the microtiter plate format for improved multiplexed microarraying. The apparatus overcomes current limitations with conventional polymeric and glass bottom microtiter plates. The invention also relates to an apparatus for conducting multiple biomolecular assays comprising a low self-fluorescent coated substrate...

20050277140 - Biomarkers of chronic pelvic pain syndrome: The present invention provides biomarkers of chronic pelvic pain syndrome for use in diagnosis, drug screening, therapy monitoring, research and therapeutic applications. In particular, the present invention provides MCP-1 and MIP-1α as biomarkers of chronic pelvic pain syndrome....

20050277124 - Cardiac conduction system cells and uses thereof: Isolation and amplification of cardiac pacemaking/conduction system cells and development of a pacemaking/conduction system in vitro using the expression of surrogate expression markers. Use of markers to identify and select for clusters of pacemaking “nodes” that are functionally coupled with adjacent contracting regions and generation of cell populations displaying electrical...

20050277154 - Combinatorial complex carbohydrate libraries and methods for the manufacture and uses thereof: A combinatorial complex carbohydrate library is provided and including a plurality of addressable complex carbohydrate structures....

20050277150 - Compositions and methods for identifying agents which regulate autolytic processes in bacteria: A nucleic acid sequence which regulates the autolytic activity of bacteria is provided. Methods for identifying and using agents which interact with the gene to inhibit bacterial growth and infectivity also are provided....

20050277116 - Compositions and methods for the identification of protein interactions in vertebrate cells: The present invention relates to a two-hybrid system for studying protein-protein interactions in mammalian host cells. The invention provides methods, reagents and kits for carrying out the two-hybrid screen. Accordingly the invention provides a bait construct that is capable of targeting a bait protein to a specific subcellular locale within...

20050277144 - Compounds and methods for fluorescent labeling: Dye reagents useful in labeling biological materials are provided along with methods for their use....

20050277143 - Constrained cis-diol-borate bioconjugation system: The invention pertains to bioconjugation systems comprising sterically constrained cis-diols and borates....

20050277115 - Corynebacterium glutamicum genes encoding proteins involved in homeostasis and adaptation: Isolated nucleic acid molecules, designated HA nucleic acid molecules, which encode novel HA proteins from Corynebacterium glutamicum are described. The invention also provides antisense nucleic acid molecules, recombinant expression vectors containing HA nucleic acid molecules, and host cells into which the expression vectors have been introduced. The invention still further...

20050277121 - Crude biological derivatives competent for nucleic acid detection: The invention relates generally to the fields of making biological unit lysates or admixtures of body fluids and of RNA analysis. More specifically, it relates to direct methods for the detection of a specific sequence of RNA in a biological unit, for example a virus, cell or tissue sample, or...

20050277126 - Cytokine receptor chain: The present invention is directed to methods for treating asthma....

20050277122 - Devices and methods for contacting fluid with a chemical array: The subject invention provides devices and methods for contacting a fluid with a chemical array. Device embodiments include a reservoir plate that includes a support having a plurality of holes therethrough alignable with a chemical array when the reservoir plate and chemical array are operatively positioned relative to each other....

20050277137 - Diagnostic multimarker serological profiling: The present invention provides a novel multianalyte LabMAP™ profiling technology that allows simultaneous measurement of multiple markers. In particular, a method is provided for diagnosing the presence of pancreatic cancer in a patient by measuring serum levels of markers in a blood marker panel comprising at least IP-10, HGF, IL-8,...

20050277117 - Drug discovery method: A method of obtaining information about a chemically active area of a target molecule, for example for drug discovery, comprising: providing a set of substantially rigid chemical gauges; reacting said target with a plurality of gauges of said set of gauges, assaying a binding of said gauges with said target...

20050277135 - Genetic diagnosis using multiple sequence variant analysis: The present invention is in the field of nucleic acid-based genetic analysis. More particularly, it discloses novel insights into the overall structure of genetic variation in all living species. The structure can be revealed with the use of any data set of genetic variants from a particular locus. The invention...

20050277125 - High-density reaction chambers and methods of use: Methods and devices for performing multiple simultaneous reactions on a reaction surface are disclosed. Methods and devices for simultaneously interrogating multiple patient samples with multiple diagnostic reagents are disclosed....

20050277127 - High-throughput method of dna immunogen preparation and immunization: A high-throughput process of generating expression-competent, antigen-encoding immunogen DNA through amplification methodology including ligation-assisted PCR is described, as well as the use of the DNA for methods of DNA immunization. Also described is an adjuvant plasmid to enhance antibody production....

20050277147 - Identifying chromosomal abnormalities in cells obtained from follicular fluid: The present disclosure describes methods for identifying chromosomal abnormalities in cells obtained from follicular fluid to identify, for example, gonadal mosaicism or low-grade gonadal mosaicism. The chromosomal abnormalities are identified using genetic analysis that can detect chromosomal, DNA or gene expression abnormalities in the cells....

20050277128 - Looped probe design to control hybridization stringency: The present invention provides a method for the generation of novel libraries of encoded magnetic particles from sub-libraries of by the generation of novel sub- libraries of magnetic nanoparticles and encoded particles. The sub-libraries are functionalized on demand are useful in the formation of arrays. The present invention is especially...

20050277131 - Mass spectrometry-based identification of proteins: The invention provides a method to C-terminally label proteins in a complex sample and identify those proteins, e.g., using mass spectrometry....

20050277134 - Method for amplifying nucleic acid and analysis of single-nucleotide polymorphism using the same: A method amplifies a nucleic acid by preparing an oligonucleotide being capable of complementarily hybridizing with a specific region of a target nucleic acid containing at least one mutation site, the oligonucleotide having at least one sequence being non-complementary to any of possible sequences of the at least one mutation...

20050277148 - Method for preparing correlation diagram or multiple alignment among nucleic acid sequences and program thereof: Means is provided by which correlation analysis among a plurality of nucleic acid sequences can be conducted in a high-speed manner on the basis of the considerations of a complementary strand of an analysis object sequence and highly accurate results can be obtained. Before conducting a correlation analysis, the directions...

20050277152 - Method for separation and purification of nucleic acid and unit for separation and purification of nucleic acid: An object of the present invention is to provide a method for separation and purification of nucleic acid, which has excellent separating properties, high washing efficiency, and easy processability, which uses mass-producible solid phases with substantially uniform separating capacities, and wherein procedures can be automated, and a unit for separation...

20050277146 - Method of amplifying template dna molecule using strand-displacing dna polymerase capable of carrying out isothermal amplification: A method of amplifying a template DNA molecule in an isothermal reaction that can reduce background noise is provided. A method of amplifying a template DNA molecule using a strand-displacing DNA polymerase capable of carrying out isothermal amplification includes a step of conducting an amplification reaction with the addition of...

20050277155 - Method of immobilizing probes, in particular for producing biochips: The invention relates to a solid support comprising sites intended for the immobilization of oligonucleotide probes or of proteins or of biological cells having ligands, the sites being provided with chemical species bound to the support by a chemical binding function. The chemical species have a diol group connected to...

20050277139 - Methods and apparatus for the detection and validation of micrornas: The present invention provides methods and apparatus for detecting, sequencing, cloning and otherwise validating nucleic acids, including microRNAs. The methods make use of a plurality of sequence-specific recognition reagents which can also be used for classification of biological samples, and to characterize the expression of certain microRNAs....

20050277118 - Methods for identifying subjects at risk of melanoma and treatments thereof: Provided herein are methods for identifying risk of melanoma in a subject, reagents and kits for carrying out the methods, methods for identifying candidate therapeutics for treating melanoma, and therapeutic methods for treating melanoma in a subject. These embodiments are based upon an analysis of polymorphic variations in a BRAF...

20050277132 - Nematode gs-like sequences: Disclosed is a nucleic acid molecule from nematodes encoding for glutamine synthetase (GS) polypeptides. The GS-like polypeptide sequence is also provided, as are vectors, host cells, and recombinant methods for production of GS-like nucleotides and polypeptides. The invention further relates to screening methods for identifying inhibitors and/or activators, as well...

20050277120 - Novel dna threading intercalators: s

20050277151 - Ntr3, a novel member of the tnf- receptor supergene family: The present invention provides a purified polynucleotide encoding a novel receptor, designated NTR3, which belongs to the TNF receptor gene superfamily; to purified NTR3 polypeptide molecules; to antibodies that bind NTR3; to materials comprising such molecules; and to methods of using such molecules....

20050277149 - Pharmaceutically tractable secondary drug targets, methods of identification and their use in the creation of small molecule therapeutics: The present invention relates to a method of identifying one or more secondary drug targets and their use in the identification of drug or drug candidates, particularly for the treatment of cancer. The yeast-based synthetic lethal screens were used to functionally identify and validate new gene targets to kill tumor...

20050277130 - Post protein hydrolysis removal of a potent ribonuclease inhibitor and the enzymatic capture of dna: The present invention concerns compositions and methods of extracting infectious pathogens from a volume of blood. In one embodiment, the method includes the steps of creating a fibrin aggregate confining the pathogens and introducing a fibrin lysis reagent to expose the pathogens for analysis. The present invention also concerns materials...

20050277141 - Process for analyzing a complex biological state of biosystem and support permitting its direct interpretation: A process for analyzing a complex biological state of a biosystem from a sample of nucleic acids isolated from the biosystem including hybridization of the nucleic acids with probes fixed on a support, including: (a) contacting marked polynucleotide sequences prepared from nucleic acids of the sample with a first set...

20050277136 - Rapid identification of nocardia farcinica: Provided herein are Nocardia farcinica-specific primers comprising the nucleotide sequence of SEQ ID NO:1-39. Provided herein is a polynucleotide represented by SEQ ID NO:41 and SEQ ID NO:40. Further provided is a method of identifying a Nocardia farcinica infection in a subject with the primer identified by SEQ ID NO:1-39,...

20050277138 - Reactive functional groups: The present invention relates to compositions and methods for the preparation of modified nucleic acids. In particular, the present invention provides novel reagents and chemistries for the generation of linkers, modified phosphoramidites, and modified solid supports....

20050277142 - Recombinant cell line for the detection of dioxin-like compounds based on the expression of luciferase gene: Disclosed herein are recombinant cell lines harboring therein an exogenous luciferase gene, in particular transfected mouse hepatoma cell lines Hepa1c1c7-M4P2 and Hepa1c1c7-MP, in which a DNA fragment having nucleotide sequences “cacgc” and “gcgtg” that may be specifically present in dioxin-response elements is located upstream of the luciferase gene, the DNA...

20050277133 - Rna interference mediated treatment of polyglutamine (polyq) repeat expansion diseases using short interfering nucleic acid (sina): The present invention concerns compounds, compositions, and methods for the study, diagnosis, and treatment of diseases and conditions associated with polyglutamine repeat (polyQ) allelic variants that respond to the modulation of gene expression and/or activity. The present invention also concerns compounds, compositions, and methods relating to diseases and conditions associated...

20050277119 - Substituted azaporphyrins as fluorescence labels: The present invention relates to marker components, fluorescent probes, oligonucleotides, hybridization assays, and immunoassays using such products, and methods for making such products. According to the present invention, detectably labeled marker components are provided that comprise a fluorescent moiety coupled to two small solubilizing groups, one on each side of...

20050277153 - Sulfotransferase sult2a1 sequence variants: Isolated sulfotransferase nucleic acid molecules that include a nucleotide sequence variant and nucleotides flanking the sequence variant are described, as well as sulfotransferase allozymes. Methods for determining if a mammal is predisposed to a heart condition or cancer also are described....

20050277123 - Variants of nedd4l associated with hypertension and viral budding: Disclosed are compositions and methods related to NEDD4L, a ubiquitin ligase, and hypertension as well as viral budding. A systematic search for genetic polymorphism was conducted by resequencing exon and intron boundaries in human genomic DNA. Isoforms encoding a Ca2+-dependent lipid binding C2 domain at the N-terminus of NEDD4L were...

20050277156 - Novel brain natriuretic peptide variants and methods of use thereof: Novel BNP variants. The novel BNP variants according to the present invention may optionally be used for diagnosis of a BNP variant-detectable disease as described herein....

20050277158 - Method and kit for donor specific complement-fixing antibodies crossmatch: Provided is a flow cytometry method and kit composition for semi-quantitatively determining complement fixing antibodies (CFAbs). The said antigen specific CFAbs in the sample will react with the antigens on the solid carriers or the surfaces of target cells as well as the labeled complement or labeled anti-complement antibody at...

20050277157 - Organ transplant rejection and associated conditions: The application discloses markers associated with organ transplant rejection and associated conditions, and in particular provides materials and methods for the diagnosis, prognosis or treatment of chronic rejection of transplanted organ such as heart and kidney. Examples of the markers include ribosomal protein L7, β-transducin, 1-TRAF (also known as TANK)...

20050277162 - Detection and modulation of cancer stem cells: The invention relates to method for detecting and modulating the expression and activity of REX-1. As described herein, REX-1 is expressed in certain cancer cells, including cancer stem cells. The invention also provides methods for detecting and/or treating cancer....

20050277161 - Phosphopeptide antigens associated with mhc molecules: The present invention describes novel tumor-specific phosphorylated peptides, nucleic acids encoding those peptides, and antibodies generated against said peptides. The genes, peptides, and antibodies described herein may be used as diagnostic indicators of the presence of cancer and/or used in therapeutics to treat cancer....

20050277163 - Methods of use of one step immunochromatographic device for streptococcus a antigen: A method to determine the presence or absence of Streptococcus Group A antigen in a sample, comprising the following steps: extracting the antigen from the sample in an assay chamber with two or less extraction reagents, wherein the two reagents may be added to the assay chamber in no particular...

20050277159 - Nanoparticle and microparticle based detection of cellular products: Embodiments of the present invention relate to devices and methods for detecting cellular products using detection particles having product-specific detection reagents and having a characteristic spectral feature. In particular, devices and methods are provided for measuring secreted cellular products including cytokines. Detection substrates, include microwells having product-specific capture reagents thereon...

20050277160 - Peptide capable of binding to nanographite structures: It is intended to provide a peptide or a phage recognizing nanographite structures and thus enabling efficient recognition, binding, separation and alignment of nanographite structures such as carbon nanohoms or carbon nanotubes, an artificial protein or a chimeric molecule comprising the above-described peptide bonded to a functional peptide, a protein,...

20050277164 - Blood glucose tracking apparatus and methods: A measurement module for glucose testing includes a glucose testing measurement module housing, a test strip receptacle formed in the housing, and a connector portion formed in the housing and shaped to permit mechanical removable attachment of the housing to a hand-held computer. Electronics determine the amount of glucose present...

20050277165 - Novel treatment: A method of screening compounds to identify those compounds which inhibit the Asp 2 mediated cleavage of a polypeptide or protein substrate, the method comprising: providing a reaction system comprising Asp 2 and substrate; and measuring the extent of cleavage of the substrate in the presence of test compound as...

20050277166 - Method for measuring polyamines in erythrocytes, diagnostic method and kit for measuring polyamines in erythrocytes: A method for measuring polyamines in erythrocytes in which a polyamine oxidase having a substrate specificity to spermine and spermidine or a polyamine oxidase having a substrate specificity to spermine only is used, an eluate in erythrocytes separated and purified from blood is reacted with these enzymes, and hydrogen peroxide...

20050277167 - Prolyl 3-hydroxylases: The invention provides prolyl 3-hydroxlase nucleic acids and proteins, methods of using such nucleic acids and proteins, and transgenic animals....

20050277168 - Apparatus and methods for scanning and producing biological specimen film strips: A biological specimen film reel, a biological specimen film assembler, and a biological specimen scanner are provided. The biological specimen film reel comprises a reel carrying a film having a plurality of biological specimens disposed thereon. The biological specimen film assembler comprises a film manipulator and a specimen transfer device....

20050277169 - Active microcosm: screening antimicrobial producing microorganisms: The Active Microcosm approach offers a new method for detecting antimicrobial producing microorganisms in natural samples prior to plating and culture isolation. The method involves inoculating portions of natural substrates; soil, litter, water etc., onto different agar nutrient media contained in the wells of a standard 96-well membrane filter plate....

20050277170 - Device and method for detecting antibiotic inactivating enzymes: The present invention provides a method of determining the antibiotic susceptibility of a microorganism comprising the following steps. First, a culture of the microorganism whose susceptibility is to be determined is admixed with an antibiotic to which susceptibility is to be assayed, and a permeabilizing agent for the microorganism present...

20050277171 - Enzymatic method to produce 7-dehydropregnenolone, vitamin d3-like compounds and derivatives thereof: Provided herein are enzymatic methods of producing steroid compounds such as 7-dehydropregnenolone, hydroxy derivatives thereof and vitamin D2- and D3-like compounds and derivatives thereof. Also provided are the derivatives of the vitamin D3-like compounds so generated via the action of cytochrome P450scc enzyme on substrates 7-dehydrocholesterol, vitamin D2 or vitamin...

20050277176 - Human and mouse beta-defensins, antimicrobial peptides: The present invention employs an iterative application of BLAST and Hidden Markov Model (HMM) based searches which identified 34 β-defensin genes in the human genome and 48 in the mouse genome. The present invention relates to novel antimicrobial peptides and derivatives thereof as well as the β-defensin genes encoding the...

20050277173 - Method for producing human antibodies with properties of agonist, antagonist, or inverse agonist: A method for obtaining agonist, antagonist and inverse agonist, to a given physiological receptor is disclosed. For the method, use is made of in silico design synthetic immunogens, which are caused to act in vitro on human lymphocyte-containing cell populations. A preferred receptor is human CD152, particularly the regions of...

20050277174 - Mutant tyrosine repressor, a gene encoding the same, and a method for producing l-dopa: A mutant tyrosine repressor that does not require tyrosine to induce expression of tyrosine phenol-lyase gene is obtained by introducing a mutation into a tyrosine repressor. A microorganism which is able to express large amounts of tyrosine phenol-lyase is obtained by introducing the mutant tyrosine repressor into the microorganism. The...

20050277172 - Novel variant hypocrea jecorina cbh1cellulases: Described herein are variants of H. jecorina CBH I, a Cel7 enzyme. The present invention provides novel cellobiohydrolases that have improved thermostability and reversibility....

20050277175 - Truncated adamts molecules: The invention provides truncated biologically active ADAMTS polypeptides, particularly those with hyalectenase activity, and more particularly those with aggrecanase activity, that exhibit greater stability and homogeneity and higher expression yields than their full-length counterparts. The invention also provides nucleic acid molecules encoding such truncated biologically active ADAMTS polypeptides and methods...

20050277178 - Embedded expression of heterologous proteins: A method for expressing a heterologous protein in a prokaryotic host cell, comprising the steps of: (a) providing a DNA vector having: (1) a control region operatively linked to a first genetic element encoding a carrier protein capable of expression in the host cell, and (2) a second genetic element...

20050277179 - L-tyrosine-producing bacterium and a method for producing l-tyrosine: The present invention describes the production of L-tyrosine by culturing in a medium an Escherichia bacterium which has L-tyrosine-producing ability and which carries a mutant prephenate dehydrogenase which is desensitized to feedback inhibition by L-tyrosine, producing and accumulating L-tyrosine in the medium or in the bacterial cells, and collecting L-tyrosine...

20050277180 - Hydrolytic process of oil and fat: wherein ΔP represents a maximum pressure loss [MPa] through said packed layer during said hydrolytic reaction, L represents a thickness [m] of said packed layer, dp represents a weight-basis average particle size [m] of particles of said packed, immobilized enzyme, and ε represents a void volume [−] of said packed...

20050277181 - Compositions and methods for detecting pathogen infection: The present invention generally features therapeutic and diagnostic compositions and methods for increasing or decreasing the binding of a lysozyme polypeptide to a Treponema pallidum P17 polypeptide (Tp17) or a Tp17-like polypeptide. More particularly, the invention relates to compositions and methods for detecting, treating, or preventing a pathogen infection or...

20050277182 - Apparatus and method for injecting substance into cell: A surface of a cell is irradiated with laser beam to make an opening in the cell membrane, and a drug fluid is inserted in the cell through the opening....

20050277183 - Electroporation cuvette: Cuvettes and methods of using cuvettes in electroporation are provided. The cuvette comprises a cuvette body, and an opening, wherein the cuvette body comprises an electrode including a pair of parallel spaced electrode plates, a cavity and a well disposed inside the electrode plates. The cuvette is configured to produce...

20050277184 - Stirring system for cell culture: A stirring system (1) is disclosed for use in a cell culture system which handles a large number of cell culture vessel assemblies (2). Each cell culture vessel assembly includes a platen (23) on which are disposed a plurality of stirring rods (25), and which is transversely movable across the...

20050277185 - Binding assay device with reservoir: A binding assay device can provide an array of parallel channels with reservoirs for accommodating varying volumes of liquid samples for assay. The device generally can comprise a substantially planar base and a substantially planar top plate with open channels formed in its bottom surface. One embodiment includes a removable...

20050277186 - Imaging system for examining biological material: Improved biological material imaging systems and related methods are provided by using an imaging system for imaging biological materials, the imaging system comprising a sensor having one or more receptors, and an image transfer medium to scale the one or more receptors to an object field of view....

20050277187 - Creation of shear in a reactor: An apparatus for performing a biological or biochemical reaction that, in certain embodiments, has the ability to apply shear stress to a component of a liquid sample and includes a biological or biochemical reactor comprising a container having a volume of less than about 2 mL and containing a liquid...

20050277188 - Fermentation flask for cultivating microorganisms: A container is provided for cell growth by artificial cultivation of selected biological material in a growth liquid for use in an oscillating incubator. The container has a circular bottom with rounded corners joining a sidewall that is inclined at an angle toward a longitudinal axis of the container to...

20050277189 - Method for stimulating self-renewal of neural stem cells and enhancing neurogenesis: The present invention provides a mechanism for obtaining an almost unlimited source of stem cells. A method for the expansion of a population of stem cells that retain their developmental capacity comprises co-culture of a stem cell population with a trophic support, for example, endothelial cells or conditioned medium derived...

20050277190 - Pancreatic stem cells: Pancreatic progenitor cells isolated from the pancreas of a mammal. The invention also includes pancreatic cells or neural cells differentiated from the pancreatic progenitor cells....

20050277191 - Fermentation flask for cultivating microorganisms, a growth media and method of use: A container is provided for cell growth by artificial cultivation of selected biological material in a growth media for use in an oscillating incubator. The container has a circular bottom with rounded corners joining a sidewall that is inclined at an angle toward a longitudinal axis of the container to...

20050277194 - Complementing cell lines: A packaging cell line that complements recombinant adenoviruses based on serotypes from subgroup B, preferably adenovirus type 35. The cell line is preferably derived from primary, diploid human cells that are transformed by adenovirus E1 sequences either operatively linked on one DNA molecule or located on two separate DNA molecules,...

20050277192 - Hepatitis c virus nonstructural protein 4a (ns4a) is an enhancer element: Aspects of the present invention concern the discovery of an enhancer that regulates the expression of an associated gene. More particularly, it was found that the nonstructural protein 4A (NS4A) from the hepatitis C virus (HCV) modulates the expression and immunogenicity of an associated nucleic acid....

20050277193 - Vectors and methods for gene transfer: The present invention provides a recombinant adenovirus comprising coat proteins that lack native binding. In particular, the present invention provides a recombinant adenovirus comprising a penton base protein and a fiber protein, wherein the penton base protein and the fiber protein lack native binding. The present invention further provides a...

20050272026 - Apparatus and method for cell analysis: A cell analysis apparatus is described that includes a test sample preparation part for preparing a test sample by treating an analyte with a dye for staining an intracellular substance of the cells in the analyte, a detector for detecting scattered light and fluorescence from each cell in the test...

20050272025 - Method of detecting and preventing alzheimer's disease, particularly at prodromal and early stages: A method of detecting Alzheimer's disease includes detecting a disruption or alteration in normal sub-cellular distribution of G-protein receptor kinases (GRKs), particularly GRK2 and GRK5. The disruption is caused by abnormal accumulation of soluble β-amyloid. The prevention or suppression of the disease progression at prodromal or early stages includes correction...

20050272028 - Myostatin and mimetics thereof: An isolated mimetic of functional myostatin comprising part of the amino acid sequence of functional myostatin, such that the mimetic is capable of inhibiting muscle growth....

20050272027 - Real-time clinical diagnostic systems for fluorescent spectrum analysis of tissue cells and methods thereof: Real-time clinical diagnostic expert systems for fluorescent spectrum analysis of tissue cells and methods thereof. An exemplary system includes a set of optical fibers, wherein the first optical fiber introduces an incident light to an subject epidermal tissue, and the second optical fiber receives an auto-fluorescent signal, a set of...

20050272029 - Hepatitis c viral-like particle purification: Methods for obtaining HCV complexes and HCV-like particles comprising HCV structural genes are provided. In one method, cells containing HCV-like particles are lysed with digitonin in the presence of protease inhibitors. Polyethylene glycol is slowly added to the lysate, to provide a precipitate that comprises complexes of the HCV structural...

20050272032 - Internal control for in situ hybridization: The invention provides a method for monitoring the quality of in situ hybridization analysis of a nuclear DNA target in a tissue or cell sample using a mitochondrial DNA probe as an internal control. The invention also provides a reagent for in situ hybridization detection of a nuclear DNA target...

20050272030 - Nucleic acid constructs for gene expression: Nucleic acid constructs comprising viral genomic nucleic acid which comprises at least two endogenous gene expression regulatory units which each comprise an endogenous promoter where the endogenous promoters of the units are active at the same phase in the viral life cycle of the virus the viral genomic nucleic acid...

20050272031 - Viral variants and uses therefor: Disclosed are viral variants exhibiting reduced sensitivity to particular agents including nucleoside analogues and immunological mediators such as immunoglobulins and immune cells. Also provided are hepatitis B virus (HBV) variants which exhibit a level of replication fitness in the presence of a nucleoside analogue similar to or greater than in...

20050272082 - Alternatively spliced isoform of acetyl-coa carboxylase 2 (acc2): The present invention features nucleic acids and polypeptides encoding novel splice variant isoforms of acetyl-CoA carboxylase 2 (ACC2). The polynucleotide sequence of ACC2sv1 is provided by SEQ ID NO 3. The amino acid sequence of ACC2sv1 is provided by SEQ ID NO 4. The present invention also provides methods for...

20050272087 - Apparatus and method for finding genes associated with diseases: A method of finding genes associated with a disease, includes: finding all potential gene symbols; folding at least one alias into official gene symbols; and computing the relevance of each official symbol to the disease. The method may further include, eliminating non-gene symbols by use of contextual clues....

20050272049 - Arrays of magnetic particles: The present invention provides a method for the generation of novel libraries of encoded magnetic particles from sub-libraries of by the generation of novel sub-libraries of magnetic nanoparticles and encoded particles. The sub-libraries are functionalized on demand are useful in the formation of arrays. The present invention is especially useful...

20050272047 - Automated process for washing array substrates: A method of washing an array substrate is disclosed. The method includes contacting the array substrate with a first wash solution and triggering an automatic wash process. The automatic wash process includes a) separating the array substrate from the first wash solution, b) contacting the array substrate with a second...

20050272062 - Bacteria detecting instrument, bacteria detecting method, and bacteria detecting kit: A micro array instrument is used in which an oligonucleotide based on a species- or genus-specific nucleotide sequence of subject bacteria is immobilized on a surface of a substrate. By confirming whether the oligonucleotide immobilized on the substrate has hybridized with a probe prepared from a test sample, bacteria contained...

20050272070 - Base specific cleavage of methylation-specific amplification products in combination with mass analysis: Methods, combinations and kits are provided for identifying the methylation state of a target nucleic acid molecule, the methylation state of a nucleotide locus in a target nucleic acid molecule, or for identifying the locus of one or more methylated or unmethylated nucleotides in a target nucleic acid molecule. Methylation...

20050272050 - Cd40 splice variants, compositions for making and methods of using the same: Provided are substantially pure CD40 splice variants that include altered internal sequences and unique tail sequences relative to previously described CD40 protein sequences. Also provided are fragments of the CD40 splice variants comprising at least 10 amino acids including at least 4 amino acids of the unique tail sequence; the...

20050272060 - Chimeric cryle delta endotoxin and methods of controlling insects: The present invention relates to a chimeric δ endotoxin protein Cry 1E of SEQ ID No. 1 with extraordinarily high insecticidal property and a chimera gene of SEQ ID No. 2 encoding the said chimeric protein, and also a method of treating insect infested plants using said chimera protein....

20050272038 - Compositions and methods for diagnosis and therapy of cancers: The present invention relates to a method for improved diagnosis of cervical lesions based on detection of gene products encoded by the INK4a gene locus. According to the present invention an improvement in diagnosis may be achieved by assessing the presence or absence or the level of overexpression of at...

20050272074 - Compositions and methods for reverse transcription: The present invention provides compositions and methods for high fidelity cDNA synthesis. In particular, the composition of the present invention contains a first enzyme exhibiting a reverse transcriptase activity and a second enzyme comprising a 3′-5′ exonuclease activity....

20050272067 - Compositions, splice variants and methods relating to cancer specific genes and proteins: The present invention relates to newly identified nucleic acid molecules and polypeptides present in normal and neoplastic cells, including fragments, variants and derivatives of the nucleic acids and polypeptides. The present invention also relates to antibodies to the polypeptides of the invention, as well as agonists and antagonists of the...

20050272089 - Critical genes and polypeptides of haemophilus influenzae and methods of use: The present invention provides methods of identifying agents that bind polypeptides critical for the survival of Haemophilus spp., preferably H. influenzae. The present invention also provides critical polypeptides and the polynucleotides encoding the critical polypeptides....

20050272077 - Cytokine-, stress-, and oncoprotein-activated human protein kinase kinases: Disclosed are human mitogen-activated (MAP) kinase kinase isoforms (MKKs). MKKs mediate unique signal transduction pathways that activate human MAP kinases p38 and JNK, which result in activation of other factors, including activating transcription factor-2 (ATF2) and c-Jun. The pathways are activated by a number of factors, including cytokines and environmental...

20050272088 - Dark quenchers for donor-acceptor energy transfer: The present invention provides a family of dark quenchers, termed Black Hole Quenchers (“BHQs”), that are efficient quenchers of excited state energy but which are themselves substantially non-fluorescent. Also provided are methods of using the BHQs, probes incorporating the BHQs and methods of using the probes....

20050272065 - Differential enzymatic fragmentation by whole genome amplification: The present invention provides methods for detecting the presence of methylation at a locus within a population of nucleic acids....

20050272083 - Egfr mutations: The present invention relates to mutations in Epidermal Growth Factor Receptor (EGFR) and methods of detecting such mutations as well as prognostic methods method for identifying a tumors that are susceptible to anticancer therapy such as chemotherapy and/or kinase inhibitor treatment. The methods involve determining the presence of a mutated...

20050272071 - Encoding and decoding reactions for determining target polynucleotides: The present invention is directed to methods, reagents, and kits for detecting the presence or absence of (or quantifying) target polynucleotide sequences in at least one sample using encoding and decoding reactions. When a particular target polynucleotide is present in a sample, a reaction product is formed in the encoding...

20050272061 - Expression profiling in non-small cell lung cancer: The present invention relates to L genes and gene products that are differentially expressed in cancer tissues and cell lines. In a particular aspect of the invention, L genes and gene products are differentially expressed in lung cancer tissues and cell lines. In accordance with the present invention, L nucleic...

20050272035 - Functional screening method: The present invention provides high-throughput functional genomic methods for determining gene and protein function in a cellular context. Also provided are methods for identifying chemical modulators of gene and protein/enzyme activity. Assays are generated in concert with screening in an iterative process which expands the scope of biological coverage with...

20050272056 - Gene identification method: A method for the identification of genes that are essential for the maintenance of specific cell phenotypes is disclosed. The method includes the initial step of identifying a cell type with a phenotype of interest. Gene inactivation is performed on an aliquot of cells of the cell type of interest....

20050272054 - Genetic polymorphisms associated with cardiovascular disorders and drug response, methods of detection and uses thereof: The present invention is based on the discovery of genetic polymorphisms that are associated with cardiovascular disorders, particularly acute coronary events such as myocardial infarction and stroke, and genetic polymorphisms that are associated with responsiveness of an individual to treatment of cardiovascular disorders with statin. In particular, the present invention...

20050272064 - Halohydrin dehalogenases and related polynucleotides: The present invention relates to novel halohydrin dehalogenase polypeptides and the polynucleotides that encode them. These polypeptides are useful in the production of 4-substituted-3-butyric acid derivatives and vicinal cyano, hydroxyl substituted carboxylic acid esters. The invention also provides related vectors, host cells and methods....

20050272084 - Homogeneous luminescence energy transfer bioassay: This invention concerns a luminescence energy transfer based homogeneous bioassay comprising a first group labelled with an energy donor and a second group labelled with an energy acceptor, wherein the donor is a luminescent lanthanide label, said label being able to up-convert a lower-energy excitation to a higher-energy emission, the...

20050272045 - Hpr6 mutants and uses thereof: Methods and agents that interfere with Hpr6 function in non-sarcoma tumor cells are disclosed. Anti-Hpr6 agents are used to enhance the killing effect of anti-cancer agents in non-sarcoma tumor cells and to teat non-sarcoma tumors....

20050272036 - Ketones: Compounds of formula (I): wherein variable groups are as defined within; for use in the inhibition of 11βHSD1 are described....

20050272072 - Ligand-dependent protein splicing: Ligand-dependent inteins allow for modulation of a protein's activity in vivo. Upon binding of the ligand to the ligand-dependent intein inserted into the protein of interest, the hybrid protein undergoes protein splicing removing the intein. The activity of the spliced protein is then restored. A 4-hydroxytamoxifen-dependent intein based on the...

20050272044 - Method and kit for the detection and/or quantification of homologous nucleotide sequences on arrays: The invention relates to a method and a kit for the specific identification and/or quantification of one or several among at least 7 organisms or parts thereof, in a biological sample being possibly contaminated by at least 4 other organisms, by detecting at least one nucleotide sequence specific of each...

20050272040 - Method for increasing synaptic growth or plasticity: The present invention relates to a method of modulating synaptic growth or plasticity by increasing the expression of genes found to be induced by BNDF. Such genes include c-fos proto-oncogene, early growth response protein 1, activity-regulated cytoskeletal associated, fos-related antigen 2, G1/S-specific cyclin D1, voltagegated potassium channel protein, sodium channel...

20050272059 - Method for providing microarrays: An interactive method of providing an array of nucleic acid sequences in which a remote user enters a query to generate a listing of desired sequence probes, which are then selected and returned to the host for use in producing a custom microarray designed by a remote user....

20050272063 - Method for treating synovial sarcoma: Methods of detecting synovial sarcoma using differentially expressed genes are disclosed. Also disclosed are methods of identifying agents for treating synovial sarcoma. Further, a method for treating or preventing a disease that is associated with Frizzled homologue 10 (FZD10) in a subject is provided....

20050272033 - Method of testing wheat: A method of testing the presence/absence of wheat in a food by performing PCR with the use of primers which have been designed on the basis of data obtained from a part of a gene of wheat. This method is highly useful in detecting a trace component contained in a...

20050272055 - Method of treating lethal shock induced by toxic agents and diagnosing exposure to toxic agents by measuring distinct pattern in the levels of expression of specific genes: A method for administering a therapeutic agent which inhibits the mechanistic pathways necessary to maintain the progression of lethal shock. The therapeutic agent is administered in the form of a drug, antisense or protein depending on the gene expression....

20050272085 - Methods for forensic and congenic screening: The present invention provides a method to rapidly provide genotype screening of a plurality of samples for a microsatellite loci. The screening results have forensic and congenic applications....

20050272086 - Methods for genomic analysis: The present invention relates to methods for identifying variations that occur in the human genome and relating these variations to the genetic basis of disease and drug response. In particular, the present invention relates to identifying individual SNPs, determining SNP haplotype blocks and patterns, and, further, using the SNP haplotype...

20050272043 - Methods for identifying risk of breast cancer and treatments thereof: Provided herein are methods for identifying risk of breast cancer in a subject and/or a subject at risk of breast cancer, reagents and kits for carrying out the methods, methods for identifying candidate therapeutics for treating breast cancer, and therapeutic methods for treating breast cancer in a subject. These embodiments...

20050272080 - Methods of analysis of degraded nucleic acid samples: The invention provides arrays for analysis of compromised nucleic acid samples, for example, nucleic acids obtained from formalin fixed paraffin embedded samples and methods to analyzed these compromised samples. Arrays are disclosed in which the probe selection region used to select probes for the array is the 300 bases of...

20050272034 - Methods of detecting ovarian neoplasia: Methods for diagnosing ovarian neoplasia, monitoring ovarian cancer therapy, and monitoring progression of disease in subjects based on the level of the proform of major basic protein in biological samples from the subjects are described....

20050272051 - Methods of preventing or treating recurrence of myocardial infarction: Linkage of myocardial infarction (MI) with a locus on chromosome 12q23 is disclosed. In particular, the LTA4H gene within this locus is shown by association analysis to be a susceptibility gene for MI. Methods for preventing and/or treating the recurrence of MI, in particular are described....

20050272046 - Methods, apparatus and compositions for improved measurements with optical biosensors: The invention provides an apparatus for interrogating an optical biosensor. The apparatus includes (a) locating means for positioning one or more optical biosensors; (b) calibration means for generating a reference measurement; (c) one or more biosensors for generating one or more sample measurements, the biosensors being substantially planar and removable...

20050272081 - Methods, compositions and apparatus for making nucleic acid molecules having a selected affinity to a target molecule: The present invention is directed to methods, compositions, kits and devices for making a nucleic acid having selected affinity to a target molecule. One embodiment of the present invention is a method of making a replicatable nucleic acid template having a selected affinity to a target. The method comprises the...

20050272069 - Methylthioadenosine phosphorylase compositions and methods of use in the diagnosis and treatment of proliferative disorders: Disclosed are novel nucleic acid and peptide compositions comprising methylthioadenosine phosphorylase (MTAP) and methods of use for MTAP amino acid sequences and DNA segments comprising MTAP in the diagnosis of human cancers and development of MTAP-specific antibodies. Also disclosed are methods for the diagnosis and treatment of tumors and other...

20050272052 - Molecular genetic profiling of gleason grades 3 and 4/5 prostate cancer: Many genes are affected in prostate cancers which have not been previously identified. This includes genes that have been up-regulated or down-regulated. Monitoring the expression levels of these genes is useful to identify the existence of prostate cancer. Also, monitoring the expression levels of these genes is useful to predict...

20050272092 - Novel gene having reverse transcriptas motif: The present invention aims to provide a novel gene having a reverse transcriptase motif. The invention isolates a novel gene having a reverse transcriptase motif, and gives its complete base sequence determined. The invention also provides a protein encoded by the gene, and an antibody against the protein. The use...

20050272075 - Novel methods for quantification of micrornas and small interfering rnas: The invention relates to ribonucleic acids, probes and methods for detection, quantification as well as monitoring the expression of mature microRNAs and small interfering RNAs (siRNAs). The invention furthermore relates to methods for monitoring the expression of other non-coding RNAs, mRNA splice variants, as well as detecting and quantifying RNA...

20050272066 - Novel ubiquitin ligases as therapeutic targets: The present invention relates to the discovery, identification and characterization of nucleotides that encode novel substrate-targeting subunits of ubiquitin ligases. The invention encompasses nucleotides encoding novel substrate-targeting subunits of ubiquitin ligases: FBP1, FBP2, FBP3, FBP4, FBP5, FBP6, FBP7, FBP8, FBP9, FBP10, FBP11, FBP12, FBP13, FBP14, FBP15, FBP16, FBP17, FBP18, FBP19,...

20050272039 - Nucleic acid analysis chip and nucleic acid analyzer: Means for performing direct quantitative analysis of the components of nucleic acids of cell 103 placed in a well as culture container (102) of a cell culture microchamber, comprising means for optically measuring each of the culture containers (102) of cell culture microchamber, means for effecting optical heating, means for...

20050272090 - Nucleic acid binding of multi-zinc finger transcription factors: A method of identifying transcription factors comprising providing cells with a nucleic acid sequence at least comprising a sequence CACCT (SEQ ID NO:1) as bait for the screening of a library encoding potential transcription factors and performing a specificity test to isolate said factors. Preferably, the bait comprises twice the...

20050272053 - Oligonucleotides labeled with a plurality of fluorophores: An embodiment of the invention discloses new methods for designing labeled nucleic acid probes and primers by labeling oligonucleotides with a plurality of spectrally identical or similar dyes and optionally with one or more quencher dyes. Oligonucleotides labeled in accordance with some embodiments of the invention exhibit a detectable increase...

20050272078 - Peptic nucleic acid probes for analysis of enterococcus faecium: Disclosed are PNA based probes that include a nucleobase sequence suitable for the analysis of Enterococcus faecium. In one embodiment, the probe is complementary to a target sequence of Enterococcus faecium 23 rRNA or rDNA or its complement. The invention has a wide range of important applications including use to...

20050272076 - Photolabile protecting groups: wherein R1═H, halogen, NO2, CN, OCH3, an alkyl, alkoxy or alkoxyalkyl residue having 1 to 4 C atoms, preferably a methyl, ethyl, propyl or butyl residue or an optionally substituted aryl residue or aliphatic acyl residue having 2 to 5 atoms, R2 to R7═H, NO2, CN, OCH3, a branched or...

20050272073 - Ploidy classification method: Image analysis methods and apparatus are used for determination of the ploidy of cells. The methods may involve segmenting an image to identify one or more discrete regions occupied by cells or nuclei, determining the presence of a particular ploidy indicator feature within the region(s), and providing a value of...

20050272091 - Polynucleotides and polypeptides basb033 from neisseria meningitidis and their uses: The invention provides BASB033 polypeptides and polynucleotides encoding BASB033 polypeptides and methods for producing such polypeptides by recombinant techniques. Also provided are diagnostic, prophylactic and therapeutic uses....

20050272041 - Purified plant expansin proteins and dna encoding same: This invention relates to a new class of proteins called expansins, and methods related thereto are presented. This class of proteins can be characterized as catalysts of the extension of plant cell walls and the weakening of the hydrogen bonds in pure cellulose. Two proteins have been isolated from washed...

20050272048 - Ricin-like toxin variants for treatment of cancer, viral or parasitic infections: The present invention provides a protein having an A chain of a ricin-like toxin, a B chain of a ricin-like toxin and a heterologous linker amino acid sequence, linking the A and B chains. The linker sequence contains a cleavage recognition site for a disease specific protease such as a...

20050272058 - Secreted and transmembrane polypeptides and nucleic acids encoding the same: The present invention is directed to novel polypeptides and to nucleic acid molecules encoding those polypeptides. Also provided herein are vectors and host cells comprising those nucleic acid sequences, chimeric polypeptide molecules comprising the polypeptides of the present invention fused to heterologous polypeptide sequences, antibodies which bind to the polypeptides...

20050272042 - Sequential addition of short dna oligos in dna-polymerase-based synthesis reactions: A method of fabricating a DNA molecule of user-defined sequence. The method comprises the steps of preselecting a multiplicity of DNA sequence segments that will comprise the DNA molecule of user-defined sequence, separating the DNA sequence segments temporally, and combining the multiplicity of DNA sequence segments with at least one...

20050272057 - Small segments of dna determine animal identity and source: Methods and compositions to trace an animal to a country or a farm of origin or to identify an animal based on SNPTrack analyses are described. Methods of developing SNPTracks that include a plurality of markers with one or more SNPs and SNPTrack analysis kits are described....

20050272037 - System and method for solution based multiparameter analysis of analytes: There is described a system for multiparameter analysis of analytes. The system comprises: 1) primary supports (1) with a largest dimension (3) of 500 μm or less suspended in use in a fluid solution; 2) each primary support (1) comprises an identification means (2) for identification thereof; 3) at least...

20050272079 - Thermal microvalves: The movement and mixing of microdroplets through microchannels is described employing silicon-based microscale devices, comprising microdroplet transport channels, reaction regions, electrophoresis modules, and radiation detectors. The discrete droplets are differentially heated and propelled through etched channels. Electronic components are fabricated on the same substrate material, allowing sensors and controlling circuitry...

20050272068 - Uch-l1 expression and cancer therapy: Methods are provided of treating cancer with one or more farnesyl transferase inhibitors. Methods are provided for identifying cancers that are particularly susceptible to treatment with one or more farneseyl transferase inhibitors by identifying cancers that express low levels of UCH-L1....

20050272093 - Assays for screening compounds which interact with cation channel proteins, mutant prokaryotic cation channel proteins, and uses thereof: Assays for screeing potential drugs or agents that can interact and potentially bind to cation channel proteins, and potentially have uses in treating conditions related to the function of cation channel proteins is provided, along with prokaryotic cation channel proteins mutated to mimic eukaryotic cation channels, which can then be...

20050272095 - Methods of identifying biomarkers: The invention provides methods of isolating and identifying biological markers of diabetes and metabolic syndrome and complications of these disease states. The biomarkers are useful in the evaluation, diagnosis and monitoring of these diseases....

20050272094 - Purified antigen for alzheimer's disease and methods of obtaining and using same: The invention relates, among other things, a preparation comprising Alzheimer's disease antigen (A68), as well as methods of obtaining this purified antigen, and methods of using this purified antigen, for instance, for diagnosing Alzheimer's disease and for detectiing human autoantibodies to the Alzheimer disease antigen. The antigen preparation according to...

20050272096 - Amphiphilic mediated sample preparation for micro-flow cytometry: A flow cytometer includes a flow cell for detecting the sample, an oil phase in the flow cell, a water phase in the flow cell, an oil-water interface between the oil phase and the water phase, a detector for detecting the sample at the oil-water interface, and a hydrophobic unit...

20050272099 - Androgen receptor complex-associated protein: The invention relates to new proteins that bind to and aide the transactivation activity of androgen receptors, and nucleic acids encoding them....

20050272097 - Methods and compositions for detecting and treating autoimmune diseases: Diseases caused by or affected by specific antibodies and/or T lymphocytes that complex with self-molecules in a subject are detected by identifying antigen specific antibodies and/or effector T lymphocytes against the antigen, in a biological fluid of an affected subject. This identification opens up treatment possibilities, for example, by desensitization....

20050272098 - Quantitation of endothelial microparticles: The present invention provides a method for determining the presence of atherosclerosis in a patient and a method for measuring atherosclerotic burden in a patient diagnosed with atherosclerosis. The methods use a two-color flow cytometric technique to quantitate the absolute number of endothelial microparticles (EMPs) in vitro using a known...

20050272103 - Cell separation matrix: A novel modified matrix system, mimicking a metastatic environment, that can be used to capture and detect viable cancer and normal cells from tissue fluid samples derived from cancer subjects and which provides effective cell separation for diagnostic and therapeutic applications in treating patients with metastatic diseases....

20050272102 - Method for diagnosis of prostate cancer: Methods and kits useful for diagnosis of prostate cancer are disclosed, based on levels of Human Carcino Antigen in semen or other biological samples....

20050272105 - Bacterial biosensors: A real-time, portable peptide-containing potentiometric biosensor that can directly identify bacterial spores. Two peptides for specific recognition of B. subtilis and B. anthracis Sterne may be immobilized by a polysiloxane monolayer immobilization (PMI) technique. The sensors translate the biological recognition event into a potential change by detecting, for example, B....

20050272104 - Method for detection of mycobacterium tuberculosis antigens in biological fluids: A method for detection of mycobacterium tuberculosis antigens in biological fluids provides immunoassay methods, diagnostic kits, and an immunochromatoraphic assay device for detection of Mycobacterium tuberculosis antigens in biological specimens, preferably body fluids and tissues. The preferred body fluids are blood, serum, plasma, urine, pulmonary fluid, sputum, cerebrospinal fluid, and...

20050272106 - Methods and kits for detection of multiple pathogens: The present invention relates to methods of simultaneously detecting multiple infectious organisms, including methods of simultaneously determining the presence or absence in a sample of one or more members of a group of pathogens including at least one viral pathogen and at least one non-viral pathogen. The present invention also...

20050272100 - Separation and quantification of biotinylated macromolecules: The present invention provides a biotin-binding membrane comprising covalently-linked biotin-binding agents, such as avidin and streptavidin, and a kit comprising the membrane. Also provided are methods for the detection and quantification of biotinylated molecules....

20050272101 - Method for the early detection of renal injury: A method and kit for detecting the immediate or early onset of renal disease and injury, including renal tubular cell injury, utilizing NGAL as an immediate or early on-set biomarker in a sample of blood serum. NGAL is a small secreted polypeptide that is protease resistant and consequently readily detected...

20050272107 - Intracellular antibodies: The invention related to intracellular single domain immunoglobulins, and to a method for determining the ability of an immunoglobulin single domain to bind to a target in an intracellular environment, comprising the steps of: a) providing a first molecule and a second molecule, wherein stable interaction of the first and...

20050272109 - Method for immobilizing conjugates in diagnostic tests: The invention concerns a method for the detection of an analyte in a sample using analyte-specific conjugates which have at least one heterologous group for an analyte-independent binding to a control zone. The present invention additionally provides new conjugates and reagent kits....

20050272108 - Stabilized two component system for chemiluminescent assay in immunodiagnostics: The present invention provides stabilized chemiluminescent formulations for use in in vitro diagnostics, including competitive as well as sandwich-type immunological assays. The stabilized assay system may be composed of two components, where the first component may contain a chemiluminescent organic compound, an enhancer, a homogenizing agent, and a suitable buffer...

20050272110 - Supersensitive immunoassays: An assay exhibiting improved sensitivity for achieving a factor of ten times (10×) better sensitivity than current sensitivity levels achieved by prior-art immunoassays, as exemplified by ELISA. Three main implementations are described: IA/MPD, Super-ELISA and Reverse Geometry Immunoassay, including specific panel biomarker relationships for the early detection and recognition patterns...

20050272111 - Renilla reniformis fluorescent proteins, nucleic acids encoding the fluorescent proteins and the use thereof in diagnostics, high throughput screening and novelty items: Isolated and purified nucleic acids encoding green fluorescent proteins from Renilla reniformis and the green fluorescent protein encoded thereby are also provided. Mutants of the nucleic acid molecules and the modified encoded proteins are also provided. Compositions and combinations comprising the green fluorescent proteins and/or the luciferase are further provided....

20050272112 - Direct assay of cholesterol in skin removed by tape stripping: Skin cholesterol is measured by applying an adhesive tape onto a selected area of the skin to adhere the tape to the selected skin area and stripping the tape off the selected skin area to obtain a sample representative of the outer stratum corneum layer of the skin, the sample...

20050272113 - Saliva test for hemolytic streptococcus: A reagent for the detection of an extracellular enzymatically active protein produced by a beta-hemolytic streptococcus bacteria found in a host biological fluid includes a proteinaceous substrate or a cholesterol-containing membrane substrate for the extracellular protein. The substrate is nonspecific within the groups of beta-hemolytic streptococcus bacterium and is in...

20050272114 - Substrates for covalent tethering to proteins: A mutant hydrolase optionally fused to a protein of interest is provided. The mutant hydrolase is capable of forming a bond with a substrate for the corresponding nonmutant (wild-type) hydrolase which is more stable than the bond formed between the wild-type hydrolase and the substrate and has at least two...

20050272115 - Examination of harmful bacterium in food or drink: The present invention relates to a method capable of detecting proliferative bacteria in food and beverage, particularly acid or neutral beverage at low cost within a short time, a detection kit which enables simple measurement, and a technology for examination of harmful bacteria in food and beverage with respect to...

20050272119 - 2', 5'-oligoadenylate phosphodiesterase: Polypeptides having 2′,5′-oligoadenylate phosphodiesterase activity, antibodies against the polypeptides, polynucleotides coding for the polypeptides, recombinant plasmid DNAs into which the polynucleotides have been inserted, host cells transformed by the recombinant plasmid DNAs, processes for screening for 2′,5′-oligoadenylate phosphodiesterase activity inhibitor substances, and processes for screening for 2′,5′-oligoadenylate phosphodiesterase expression regulation...

20050272128 - Antibody glycosylation variants having increased antibody-dependent cellular cytotoxicity: The present invention relates to the field of glycosylation engineering of proteins. More particularly, the present invention relates to glycosylation engineering to generate proteins with improved therapeutic properties, including antibodies with increased antibody-dependent cellular cytotoxicity....

20050272120 - Compositions and methods for the diagnosis and treatment of tumor: The present invention is directed to compositions of matter useful for the diagnosis and treatment of tumor in mammals and to methods of using those compositions of matter for the same....

20050272117 - Method: A method for optimized refolding of a protein which method comprises selecting for the protein at least three control factors which affect refolding of the protein and at least three levels for each control factor, making a Taguchi matrix of the control factors and levels, conducting experiments as required by...

20050272125 - Method for making acylated polypeptides: The present invention related to a method of producing polypeptides in transformed host cells by expressing a precursor molecule of the desired polypeptide which are to be acylated in a subsequent in vitro step. The invention is also related to DNA-sequences, vectors and transformed host cells for use in the...

20050272118 - Novel composition and methods for the treatment of immune related diseases: The present invention relates to compositions containing a novel protein and methods of using those compositions for the diagnosis and treatment of immune related disease...

20050272122 - Novel human g-protein coupled receptor: The present invention relates to novel identified polynucleotides, polypeptides encoded by them and to the use of such polynucleotides and polypeptides, and to their production. More particularly, the polynucleotides and polypeptides of the present invention relate to the G-protein coupled receptor family, referred to as IGS4-family. The invention also relates...

20050272123 - Novel human g-protein coupled receptor: The present invention relates to novel identified polynucleotides, polypeptides encoded by them and to the use of such polynucleotides and polypeptides, and to their production. More particularly, the polynucleotides and polypeptides of the present invention relate to the G-protein coupled receptor family, referred to as IGS4-family. The invention also relates...

20050272127 - Novel polypeptide and gene encoding the same: A polypeptide having the amino acid sequence as set forth in SEQ ID NO: 2, 4, or 6, a DNA encoding the same, and an antibody against said polypeptide, and the use thereof. The above amino acid sequence has a homology with chondromodulin-I that has an effect of controlling the...

20050272116 - Polymer substrate for recombinant protein purification and environmental remediation: A polymer substrate for recombinant protein purification is provided. A process for purifying recombinant proteins is also provided. In general, the present invention is directed to a chelating composition which may be used in a self-supported purification medium for recombinant protein purification. In another embodiment, a process for removal of...

20050272124 - Polypeptide production in animal cell culture: A method of producing a polypeptide in fed batch cell culture is provided which involves an initial cell growth phase and a distinct production phase. In the initial growth stage, animal cells having nucleic acid encoding the polypeptide are cultured at a starting osmolality of about 280-330 mOsm in the...

20050272121 - Site specific incorporation of heavy atom-containing unnatural amino acids into proteins for structure determination: Translation systems and other compositions including orthogonal aminoacyl tRNA-synthetases that preferentially charge an orthogonal tRNA with an iodinated or brominated amino acid are provided. Nucleic acids encoding such synthetases are also described, as are methods and kits for producing proteins including heavy atom-containing amino acids, e.g., brominated or iodinated amino...

20050272126 - Testis-specific differentiation-regulatory factor: A gene expressed specifically in the testis has been unexpectedly isolated in the course of studies of the expression of a gene encoding an unknown protein that triggers cell death. The isolated gene was a novel gene sequence that had no significant homologue in the database. This gene was also...

20050272129 - Transgenic fungi expressing bcl-2 and methods of using bcl-2 or portions thereof for improving biomass production, survival, longevity and stress resistance of fungi: Methods for enhancing growth in fungi and, more particularly, transgenic fungi expressing the Bcl-2 gene having improved biomass production, survival, longevity and stress resistance in solid and liquid culture are disclosed. Also disclosed are methods of using the transgenic fungi of the present invention for biocontrol....

20050272130 - Methods and compositions relating to prion-only transmission of yeast strains: Disclosed are compositions and methods related to prion-only transmission of yeast strains....

20050272131 - Methods to make and use antibodies of improved cross-reactivity: The present invention relates to methods of generating antibodies of improved cross-reactivity against antigens that give rise to immunotypic variations in infectious organisms. The methods include immunizing animals with multiple immunogen preparations that are derived from the antigen of interest. The present invention also includes methods of use of the...

20050272132 - Production of polyketides and other natural products: The present invention relates to production of polyketides and other natural products and to libraries of compounds and individual novel compounds. One important area is the isolation and potential use of novel FKBP-ligand analogues and host cells that produce these compounds. The invention is particularly concerned with methods for the...

20050272133 - Biosynthetic gene cluster for the production of a complex polyketide: A polyketide synthase complex composed of polyketide synthase with 15 total modules, a non-ribosomal peptide synthetase with 1 module, and a cytochrome P450 hydroxylase is described. Also provided are novel Streptomyces species and methods of modified Streptomyces species. Further described are novel compounds, 36-ketomeridamycin, C9-deoxomeridamycin, and C9-deoxoprolylmeridamcyin and uses thereof....

20050272134 - Production of a fermentation product: A process of producing fermentation product comprising the steps of, (i) forming an acidified suspension of particulate plant derived material comprising a first polysaccharide which is more readily hydrolysable and a second polysaccharide which is more difficult to hydrolyse, (ii) allowing the first polysaccharide to undergo hydrolysis by action of...

20050272135 - Processs for production and purification of fermentation derived organic acids: A method of producing and purifying an organic acid by producing an aqueous solution of the ammonium salt of the organic acid through fermentation and/or bioconversion and neutralization. The solution is heated to thermally crack the ammonium salt of the organic acid producing a vapor phase of ammonia and water...

20050272136 - Process for producing 3-hydroxycyclohexanone: There is provided a process for producing 3-hydroxycyclohexanone, which is characterized by the steps of reacting 1,3-cyclohexanedione with an enzyme having an ability to reduce 1,3-cyclohexanedione to 3-hydroxycyclohexanone....

20050272137 - Fermentation with a phytase: The present invention relates to an improved fermenation process wherein phytic acid-containing material is fermented in the presence of a phytase....

20050272138 - Method of preparing cross-linked enzyme particles: The invention relates to a method of preparing cross-linked enzyme particles using a cross-linking agent. According to the invention, the enzyme particles are formed and subsequently cross-linked using a cross-linking agent having at least n reactive groups where N≧3 and a molecular weight of >2,000 Da. The method according to...

20050272139 - Bacillus pumilus safr-032 isolate: Spore-forming microbes recovered from spacecraft surfaces and assembly facilities were exposed to simulated martian UV irradiation. The effects of UVA (315-400 nm), UVA+B (280-400 nm), and full spectrum (200-400 nm) on the survival of microorganisms were studied at UV intensities expected to strike the surfaces of Mars. Microbial species isolated...

20050272140 - Methods for generating hypermutable yeast: Yeast cells are mutagenized to obtain desirable mutants. Mutagenesis is mediated by a defective mismatch repair system which can be enhanced using conventional exogenously applied mutagens. Yeast cells with the defective mismatch repair system are hypermutable, but after selection of desired mutant yeast strains, they can be be rendered genetically...

20050272141 - Anti-graffiti paint formulations and removal: A paint formulation which contains specific enzymes which remain inactive in an applied surface coating of the paint formulation until specifically activated by a subsequently-applied applicator solution to facilitate removal of the surface coating. When the applicator is applied to the surface coating, the enzymes catalyse the hydrolysis of the...

20050272142 - Fluid vessel: A fluid vessel includes a base member in the form of a plate of which one surface has a concave channel and a cover plate which is bonded to the base plate so as to close an opening surface of the channel. The base member and the cover plate are...

20050272145 - Hand-held fluorescence polarimeter: A small, portable, hand-sized apparatus for detecting microorganisms or chemicals in liquid samples by fluorescence polarization. The apparatus operates using a low power excitation light source, such as an LED, in order to irradiate a sample with polarized light. Detection of emitted polarized light from the sample is detected in...

20050272144 - Micro-reactor for improving efficiency of liquid mixing and reaction: A micro-reactor is provided with a flow jointing section to joint flows of at least two kinds of liquids including a reacting reagent by feeding the two kinds of liquids with a micro pump; a mixing flow path connected to a downstream end of the flow jointing section so as...

20050272143 - Solid support comprising a functionalized electricity conductor or semiconductor surface, method for preparing same and uses thereof: The invention concerns a solid support comprising a functionalized electricity conductor or semiconductor surface coated with a functionalized electrografted organic layer wherein at least 90% of the number of functional groups of interest is accessible. The invention also concerns the method for preparing such a support and uses thereof, in...

20050272146 - Disposable bioreactor systems and methods: Accordingly, in one embodiment of the invention, a bioreactor system is presented and includes a disposable container for housing biomaterials for processing, the disposable container including at least one input port, at least one exhaust port, at least one harvest port, and the integrity of the sterile environment is protected...

20050272148 - Method of collecting placental stem cells: A method of collecting embryonic-like stem cells from a placenta which has been treated to remove residual cord blood by perfusing the drained placenta with an anticoagulant solution to flush out residual cells, collecting the residual cells and perfusion liquid from the drained placenta, and separating the embryonic-like cells from...

20050272147 - Methods for ex vivo propagation of somatic hair follicle stem cells: The present invention is directed to methods for readily propagating somatic hair follicle stem cells or melanocyte stem cells. The methods comprise enhancing guanine nucleotide (GNP) biosynthesis, thereby expanding guanine nucleotide pools. This in turn conditionally suppresses asymmetric cell kinetics in the explanted cells. The methods of the invention include...

20050272149 - Therapeutic delivery of adenosine into a tissue: The present invention relates to generation of embryonic stem cells and neural cells having deficient adenosine kinase gene on both alleles. The present invention further relates to methods of cell-based therapeutic delivery of agents and cells to a host tissue site for treatment of diseases....

20050272150 - Cultivation of hair inductive cells: The present invention relates to culturing cells which may be used in hair induction. In one aspect of the invention there is provided a method for cultivation of hair inductive cells, comprising the step of culturing the hair inductive cells in a culture medium comprising a medium conditioned by conditioning...

20050272151 - Dendritic cell line: The invention relates to plasmacytoid dendritic human cell lines (pDC), methods for the production thereof and to said cells. More specifically, said invention relates to the plasmacytoid dendritic human cell line called GEN2.2 which is deposited in CNCM under number CNCM I-2938. The use of said cells and pharmaceutical compounds...

20050272152 - Stem cell populations and methods of use: The invention provides populations of expanded CD34-expressing cells and methods of use. Particular embodiments provide for defined culture media useful for growing these cells, and grafts comprising these cells. The invention finds use in methods for reconstituting, repairing, and regenerating tissue damage....

20050272153 - Bone tissue engineering by ex vivo stem cells ongrowth into three-dimensional trabecular metal: Adult autologous stem cells cultured on a porous, three-dimensional tissue scaffold-implant for bone regeneration by the use of a hyaluronan and/or dexamethasone to accelerate bone healing alone or in combination with recombinant growth factors or transfected osteogenic genes. The scaffold-implant may be machined into a custom-shaped three-dimensional cell culture system...

20050272154 - Gene expression with covalently modified polynucleotides: A process and compound wherein nucleic acids can be modified with a host of molecules and maintain their ability to be expressed. A modifying chemical attachment of polyions to polynucleotides can be used to facilitate the change of tertiary structure of the nucleic acid and in some cases condensation of...

20050272155 - Methods and compositions for enhancing cell adhesion properties: The present invention provides a modified cell having adhesion properties that are increased as compared to the adhesion properties of an unmodified cell, comprising a) a recombinant nucleic acid encoding an integrin β3 subunit; b) a recombinant nucleic acid encoding an integrin αv subunit; c) a recombinant nucleic acid encoding...

20050272156 - System and method for electroporating a sample: A system and method are described for electroporating a sample that utilizes one or more sets of electrodes that are spaced apart in order to hold a surface tension constrained sample between the electrodes. The first electrode is connected to the lower body of the system while the second electrode...

20050266391 - Methods for preserving tissue: The invention generally relates to methods for preserving tissue, preventing reperfusion injury to implanted tissue, preventing transplant rejection or preserving a cell to be transplanted, comprising contacting tissue or a cell with an effective amount of a JNK Inhibitor. The invention further relates to compositions useful for the preservation of...

20050266390 - Processes for removing cells and cell debris from tissue and tissue constructs used in transplantation and tissue reconstruction: Methods for decellularizing mammalian tissue for use in transplantation and tissue engineering. The invention includes methods for simultaneous application of an ionic detergent and a nonionic detergent for a long time period, which may exceed five days. One method utilizes SDS as the ionic detergent and Triton-X 100 as the...

20050266392 - Central control system of specimen preservation devices: An object is to provide electrical locks in a plurality of culture devices represented by incubators and in a plurality of preservation devices represented by chemical coolers, in order to build a system based on the idea of one chamber one donor; and to provide a central control system wherein...

20050266393 - Circulating flow device for assays of cell cultures, cellular components and cell products: In vitro culture devices and methods are described. The subject methods and devices provide a means whereby cells and/or subcellular material are grown or held in a culture device that maintains the cells and/or subcellular material in a physiologically representative environment, thereby improving the predictive value of toxicity and metabolism...

20050266396 - Dielectric relaxation spectroscopy apparatus and methods of use: Methods and apparatus for measuring the dielectric relaxation properties of a sample are disclosed. Methods are disclosed for amplifying or controlling the dielectric relaxation properties of a sample by adding particles, such as functionally coated particles. In certain methods, the particles amplify or control the dielectric relaxation properties of the...

20050266394 - Magnetophoretic cell clarification: One aspect of the present invention relates to magnetophoretic devices for cell clarification. In one embodiment, the magnetophoretic device is a counter-current device, comprising a plurality of pairs of magnets selected from the group consisting of permanent magnets and electromagnets; a chain rotated by a motor; tubing; a feed inlet;...

20050266395 - Method and system for morphology based mitosis identification and classification of digital images: A method and system for morphology based mitosis identification and classification of digital images. Luminance parameters such as intensity, etc. from a digital image of a biological sample (e.g., tissue cells) to which a chemical compound (e.g., a marker dye) has been applied are analyzed and corrected if necessary. Morphological...

20050266401 - Compositions and methods for binding agglomeration proteins: Amplibody compositions and methods used to interact with agglomeration proteins are disclosed herein. Compositions herein comprise one or more DNA or RNA molecules having affinity for at least one agglomeration protein. The nucleic acid component is a naturally or non-naturally occuring molecule with twenty or more ribonucleotide bases. For RNA,...

20050266402 - Compositions and methods for binding agglomeration proteins: Amplibody compositions and methods used to interact with agglomeration proteins are disclosed herein. Compositions herein comprise one or more DNA or RNA molecules having affinity for at least one agglomeration protein. The nucleic acid component is a naturally or non-naturally occuring molecule with twenty or more ribonucleotide bases. For RNA,...

20050266399 - Hcv regulated protein expression: The present invention relates to novel host cell targets for antiviral, preferably anti-hepatitis C virus (HCV), compounds identified by analysis of HCV replicon-regulated polypeptide expression, to prognostic markers for the prediction of the outcome of a viral infection, to in vitro methods for the prediction of the outcome of a...

20050266398 - Method and device for rapid detection and quantitation of macro and micro matrices: The present invention provides a method and device for rapidly detecting the presence of analytes in a sample. Quantitative and qualitative measurements of analyte concentration in a sample may be rapidly obtained. A sample including the analyte and analyte metabolites produced by the analyte are introduced into a vessel that...

20050266397 - Methods for identification of coronaviruses: The present invention provides a method for rapid identification and quantitation of bacteria by amplification of a segment of bacterial nucleic acid followed by analysis by mass spectrometry. The compositions provide for characterization of the molecular masses and base compositions of bacterial nucleic acids which are used to rapidly identify...

20050266400 - Novel sequences encoding hepatitis c virus glycoproteins: The present invention concerns a modified nucleic acid molecule comprising a nucleotide sequence coding for a full length hepatitis C virus (HCV) glycoprotein selected from the group consisting of E1 glycoprotein and E1/E2 glycoprotein heterodimer, this molecule having at least one nucleotide alteration, wherein, due to this alteration, at least...

20050266470 - 25501, a human transferase family member and uses therefor: The invention provides isolated nucleic acids molecules, designated 25501 nucleic acid molecules, which encode novel transferase family members. The invention also provides antisense nucleic acid molecules, recombinant expression vectors containing 25501 nucleic acid molecules, host cells into which the expression vectors have been introduced, and nonhuman transgenic animals in which...

20050266469 - Alternatively spliced isoforms of checkpoint kinase 1 (chk1): The present invention features nucleic acids and polypeptides encoding two novel splice variant isoforms of checkpoint kinase 1 (CHK1). The polynucleotide sequences of CHK1sv1 and CHK1sv2 are provided by SEQ ID NO 3 and SEQ ID NO 5, respectively. The amino acid sequences for CHK1sv1 and CHK1sv2 are provided by...

20050266405 - Analysis of apoptotic bodies in bodily fluids: This invention provides reagents and methods for detecting apoptotic bodies in bodily fluid, including blood, blood plasma, and serum. The invention provides methods for labeling apoptotic bodies and protein, nucleic acid, and phospholipids components thereof. The invention further provides methods for extracting, isolating or separating apoptotic bodies from fractions of...

20050266472 - Apparatus and method for detecting bio molecule using inductance device: An apparatus and method for detecting bio molecule using an inductor element that can detect the bonding reaction between a sample and a biochip by measuring the change of the electrical characteristics of a circuit including the inductance device. The method includes forming a signal conversion unit with at least...

20050266419 - Apparatus and method for detecting genetic mutations and single nucleotide polymorphisms: A method and means of identifying nucleic acid oligomers is disclosed. A sample is split into parts and the parts are flowed through chromatography columns containing nucleic acid oligomer probes bound to a binding medium. Analyte oligomers are transiently hybridized to complementary probe oligomers during chromatography. Detection and analysis of...

20050266489 - Automated process for isolating and amplifying a target nucleic acid sequence using a rotatable transport mechanism: An automated analyzer for performing multiple diagnostic assays simultaneously includes multiple stations, or modules, in which discrete aspects of the assay are performed on fluid samples contained in reaction receptacles. The analyzer includes stations for automatically preparing a specimen sample, incubating the sample at prescribed temperatures for prescribed periods, preforming...

20050266478 - Biochips including ion transport detecting structures and methods of use: The present invention recognizes that the determination of an ion transport function or property using direct detection methods, such as patch-clamps, whole cell recording or single channel recording, are preferable to methods that utilize indirect detection methods, such as FRET based detection system. The present invention provides biochips and methods...

20050266467 - Biomarkers for multiple sclerosis and methods of use thereof: Disclosed are biomarkers, the expression of which is differentially regulated in subjects with multiple sclerosis (MS) as compared to subjects that do not have MS. Also described are methods of identification of such biomarkers, and methods of using such biomarkers as targets for the development and identification of therapeutic compounds...

20050266427 - Biomolecule/polymer conjugates: The present invention is directed to methods for immobilizing natural or synthetic biomolecules to surfaces. The methods comprise covalently linking the natural or synthetic biomolecule to a mono- or bifunctional polymer covalently and/or electrostatically immobilizing the biomolecule/polymer conjugate to an unmodified or modified surface. The biomolecule is an oligonucleotide, a...

20050266434 - Biosynthetic gene cluster for ambruticins: Domains of ambruticin polyketide synthase and modification enzymes and polynucleotides encoding them are provided. Methods to prepare ambruticin in pharmaceutically useful quantities are described, as are methods to prepare ambruticin analogs and other polyketides using the polynucleotides encoding ambruticin synthase domains or modifying enzymes....

20050266464 - C1q family member proteins with altered immunogenicity: The present invention relates to non-naturally occurring variant C1q Super Family member proteins with reduced immunogenicity. More specifically, the present invention relates to variant adiponectin and CTRP1 proteins with reduced immunogenicity....

20050266414 - Carrier tests for albinism in the cat: The present invention provides compositions and methods for detecting mutations associated with albinism in the cat....

20050266421 - Claudin polypeptides, polynucleotides, and methods of making and use thereof: This invention relates to new members of the human Claudin polypeptide family, to methods of making such polypeptides, and to methods of using them to treat Claudin-associated conditions and to identify agents that alter Claudin polypeptide activities....

20050266479 - Cloning, expression and characterization of the spg4 gene responsible for the most common form of autosomal dominant spastic paraplegia: The invention concerns the identification and characterization of the SPG4 gene encoding spastin, and some mutations thereof responsible for the most frequent form of autosomal dominant familial spastic paraplegia, to the cloning and characterization of its cDNA and the corresponding polypeptides. The invention also concerns vectors, transformed cells and transgenic...

20050266407 - Combinatorial decoding of random nucleic acid arrays: The invention provides methods and compositions for combinatorially decoding arrays....

20050266471 - Common marmoset-derived beta-actin gene and use thereof: The present invention provides a common marmoset-derived beta-actin gene and use thereof....

20050266474 - Common marmoset-derived glyceraldehyde-3-phosphate dehydrogenase gene and use thereof: The present invention provides a common marmoset-derived glyceraldehyde-3-phosphate dehydrogenase gene and use thereof....

20050266409 - Compositions and methods for diagnosing, preventing, and treating cancers: Compositions and methods for diagnosing, preventing, and treating cancers. In one embodiment, genes differentially expressed in colon, lung, breast and prostate cancer tissues relative to corresponding cancer-free tissues are identified. These genes or their products can be used as markers for the detection of respective cancers. Modulators of these genes...

20050266437 - Compositions, kits, and methods for identification, assessment, prevention and therapy of breast and ovarian cancer: The invention relates to newly discovered nucleic acid molecules and proteins associated with breast or ovarian cancer. Compositions, kits, and methods for detecting, characterizing, preventing, and treating human breast or ovarian cancers are provided....

20050266426 - Confluence regulated adhesion molecules useful in modulating vascular permeability: The invention relates to new polypeptide in isolated form belonging to a subfamily of the human Immunoglobulin Superfamily, which polypeptide shows at least 70% sequence homology with the amino acid sequence of the murine Confluency Regulated Adhesion Molecules 1 or 2 (CRAM-1 or CRAM-2) as depicted in FIG. 3 upper...

20050266485 - Cytokine receptor zcytor19: Novel polypeptides, polynucleotides encoding the polypeptides, and related compositions and methods are disclosed for zcytor19, a novel class II cytokine receptor. The polypeptides may be used within methods for detecting ligands that stimulate the proliferation and/or development of hematopoietic, lymphoid and myeloid cells in vitro and in vivo. Ligand-binding receptor...

20050266404 - Detection method of nucleic acid hybridization: The present invention relates to a detection method of DNA hybridization comprising: (a) preparing a oligo-plate by fixing capture probes onto a working electrode surface, (b) hybridizing the capture probes with target probes, (c) reacting a nucleic acid binding material specific to single-stranded nucleic acid or a double-stranded nucleic acid,...

20050266430 - Diagnostic method for cancer characterized in the detection of the deletion of g-csf exon 3: Disclosed are a method, a composition, a microarray, an antibody and a kit for diagnosis and prognosis of cancer, based on detection of deletion of the exon 3 region of G-CSF gene or levels of a mutated G-CSF protein having a deletion of an amino acid sequence corresponding to the...

20050266460 - Dna encoding hyaluronan synthase from pasteurella multocida and methods of use: The present invention relates to a nucleic acid segment having a coding region segment encoding enzymatically active Pasteurella multocida hyaluronate synthase (PmHAS), and to the use of this nucleic acid segment in the preparation of recombinant cells which produce hyaluronate synthase and its hyaluronic acid product....

20050266476 - Dual bead assays including covalent linkages for improved specificity and related optical analysis discs: The invention provides compositions and methods for determining whether a target agent is present in a biological sample. The invention provides a sensitive and consumer friendly system for performing biological assays. More particularly, the invention is directed to the use of a biodisc in a dual bead assay designed to...

20050266456 - Field-switch sequencing: The present invention provides novel compositions, methods and apparatus for DNA sequencing that can be performed, e.g., in a two-electrode chamber. The present invention also provides a method for sequencing a nucleic acid comprising immobilizing a plurality of complexes comprising a target nucleic acid, a primer nucleic acid, and a...

20050266422 - Fluoroalkoxy, nucleosides, nucleotides, and polynucleotides: The present invention related to fluoroalkoxy (“—OCF3”) nucleosides, nucleotides, and polynucleotides comprising fluoroalkoxy nucleotides. The present invention also relates to methods of synthesizing fluoroalkoxy nucleosides, nucleotides, and polynucleotides comprising fluoroalkoxy nucleotides. The present invention also relates to compounds, compositions, and methods for the study, diagnosis, and treatment of traits, diseases...

20050266438 - Genetic networks regulated by attenuated gh/igf1 signaling and caloric restriction: The invention is based on the discovery that the growth hormone-insulin-like growth factor-1 genetic signaling pathway and caloric restriction in conjunction extend lifespan and delay the onset of age-related diseases....

20050266432 - Haplotype markers for diagnosing susceptibility to immunological conditions: The invention provides a method of identifying a SNP haplotype that correlates with an HLA type, MHC allele or susceptibility to a particular disease or condition including the steps of (a) providing the identity of the nucleotide for each of a set of single nucleotide polymorphism (SNPs) in the major...

20050266487 - High fidelity thermostable ligase and uses thereof: The present invention is directed to a mutant thermostable ligase having substantially higher fidelity than either T4 ligase or Thermus thermophilus ligase. The ligase of the present invention is a mutant of a wild-type thermostable ligase having a histidine adjacent a KXDG motif, where the mutant thermostable ligase has a...

20050266492 - High resolution typing system for pathogenic mycobacterium tuberculosum: MLVA methods for strain discrimination among Mycobacterium tuberculosum strains are disclosed. Nine VNTR loci have been identified from genomic sequences of Mycobacterium tuberculosum strains and primer pairs suitable for amplifying the VNTR by PCR are disclosed. Polymorphisms at these loci were used to resolve genotypes into distinct groups. This sub-typing...

20050266446 - Human itpase-related gene variants associated with lung cancers: The invention relates to the nucleic acid sequences of two novel human ITPase-related gene variants (ITPA1 and ITPA2) and the polypeptides encoded thereby. The invention also relates to the process for producing the polypeptides encoded by the ITPA1 and ITPA2. The invention further relates to the use of the nucleic...

20050266442 - Immortalized human tuberous sclerosis null angiomyolipoma cell and method of use thereof: Disclosed are immortailized cells and cell lines that do not express the Tuberous Sclerosis Complex(TSC)-2 gene. Also sisclosed are methods of detecting TSC-related disorders using differentially expressed genes....

20050266465 - Interferon-alpha polypeptides and conjugates: The present invention provides interferon-alpha polypeptides and conjugates, and nucleic acids encoding the polypeptides. The invention also includes compositions comprising these polypeptides, conjugates, and nucleic acids; cells containing or expressing the polypeptides, conjugates, and nucleic acids; methods of making the polypeptides, conjugates, and nucleic acids; and methods of using the...

20050266433 - Magnetic device for isolation of cells and biomolecules in a microfluidic environment: The present invention features a new and useful magnetic device and methods of its use for isolation, enrichment, and purification of cells, proteins, DNA, and other molecules. In general the device includes magnetic regions or obstacles to which magnetic particles can bind. The chemical groups, i.e., capture moieties, on the...

20050266462 - Magnetic glass particles, method for their preparation and uses thereof: This invention relates to magnetic particles having a glass surface which are substantially spherical. This invention also relates to methods for making them, as well as to suspensions thereof and their uses for the purification of DNA or RNA in particular in automated processes....

20050266429 - Magnetic pigment: Magnetic particles with an outer glass surface being essentially poreless or having pores of a diameter of less then 10 nm as well as ferromagnetic particles with a glass surface are preferentially useful for the isolation of biological material from samples. They provide a quick and reliable purification....

20050266480 - Mammalian cytokine-like factor 7: Novel mammalian zcyto7 polypeptides, polynucleotides encoding the polypeptides, and related compositions and methods including antibodies and anti-idiotypic antibodies....

20050266406 - Maxs as modifiers of the axin pathway and methods of use: Human MAX genes are identified as modulators of the AXIN pathway, and thus are therapeutic targets for disorders associated with defective AXIN function. Methods for identifying modulators of AXIN, comprising screening for agents that modulate the activity of MAX are provided....

20050266455 - Method and microelectronic device for multi-site molecule detection: A method and apparatus are provided for identifying molecular structures within a sample substance using an integrated circuit array sensor having a plurality of test sites upon which the sample substance is applied. Each test site includes a probe formed therein to bond with an associated target molecular structure. An...

20050266408 - Method for analysing a polynucleotide: A method of analysing a polynucleotide target involves incubating the target with an oligonucleotide probe, generally an array of immobilised oligonucleotide probes, to form a duplex, and using ligase or polymerase to extend one chain of the duplex. A point mutation or variable number tandem repeat section may be analysed....

20050266450 - Method for analyzing a target nucleic acid fragment and a kit for analyzing a target nucleic acid fragment: An object of the present invention is to provide a method for analyzing a target nucleic acid fragment which can be simply and swiftly carried out by using a small apparatus, a kit for analyzing a target nucleic acid fragment using the method for analysis, and a dry analytical element...

20050266403 - Method for analyzing base sequence of nucleic acid: Provided is a method for performing a hybridization reaction that comprises the steps of providing a sample containing a target single-stranded nucleic acid and a probe array; heat-denaturing the probe array in a solution containing the sample; and reducing temperature to the extent suitable for a double-strand formation reaction, wherein...

20050266475 - Method for determining an analyte: o

20050266463 - Method for generating hypermutable plants: Blockade of mismatch repair in a plant can lead to hypermutation and a new genotype and/or phenotype. One approach used to generate hypermutable plants is through the expression of dominant negative alleles of mismatch repair genes in transgenic plants or derived cells. By introducing these genes into cells and transgenic...

20050266447 - Method for identifying activators of gene transcription: Methods and compositions for identifying a nucleotide sequence that encodes a polypeptide that directly or indirectly modulates the activity of a transcriptional regulatory region are provided. The methods permit the efficient screening of a large number of candidate nucleotide sequences for potential modulators of transcription. In particular, a population of...

20050266413 - Method for identifying location on an optical disk: A method is disclosed for positioning an optical labeling mechanism substantially close to a particular track of a labeling surface on an optical disk. The method can include the operation of positioning the optical labeling mechanism adjacent to the particular track. A further operation can be measuring a first surface...

20050266451 - Method for in vitro molecular evolution of protein function: The present invention relates to a method for in vitro creation of molecular libraries evolution of protein function. Particularly, it relates to variability and modification of protein function by shuffling polynucleotide sequence segments. A protein of desired characteristics can be obtained by incorporating variant peptide regions (variant motifs) into defined...

20050266415 - Method for sampling gas-borne matter: A method for sampling gas-borne matter using a collection device comprising an inlet, an outlet, and a slide provided adjacent the inlet includes drawing a gas through the collection device such that gas-borne matter is collected on the slide, sealing at least one of the inlet and the outlet, and...

20050266468 - Method for the direct detection of diagnostic rna: Methods for the direct detection of diagnostic target RNA have been developed, which obviate the need for time consuming RNA purification and isolation procedures....

20050266412 - Method of amplifying infectious protein: Infectious proteins such as prions present in a sample are amplified by adding a recombinant form (or portion thereof) of the infectious protein to the sample. The sample with the recombinant protein therein is maintained under cell free conditions which promote amplification for 20 hours or less and then assayed...

20050266449 - Method of detecting a hybrid, formed of at least two species, on a substrate: The present invention relates to a label-free method of detecting a hybrid, such as a double stranded nucleic acid, a protein—DNA complex or an antibody—antigen-complex, formed by at least two species, such as two single stranded nucleic acids, a protein and a nucleic acid, or an antibody and an antigen,...

20050266448 - Method of nucleic acid analysis, nucleic acid analysis device and disk for nucleic acid analysis: There is provided a method or structure of carrying out optical measurement for the change of the state of the reactive sample solution at two or more measurement points of the channel simultaneously and continuously in a flow system reaction in which the reactive proceeds according to the elapsed time...

20050266488 - Method to improve translation of polypeptides by using untranslated regions from heat-shock proteins: Untranslated regions associated with the heat shock response can be used to obtain increased efficiency of translation of polypeptides that are not necessarily normally associated with the heat shock response. This allows the development of greatly improved expression systems. The invention is also useful, for example, in the treatment of...

20050266458 - Methods and kits for methylation detection: Ligation-based methods and kits are disclosed for determining the degree of methylation of one or more target nucleotides. In certain embodiments, the methylation status of one or more target nucleotides is determined by generating misligation products. In certain embodiments, at least one target nucleotide is amplified prior to the ligation...

20050266417 - Methods for identifying target nucleic acid molecules: The present invention relates to methods for identifying target nucleic acid molecules differing by one or more single-base changes, insertions, deletions, or translocations; and identifying one or more target mRNA molecules differing by one or more splice site variations in a plurality of mRNA molecules. Also disclosed is a method...

20050266425 - Methods for producing and identifying multispecific antibodies: The present invention relates to a high efficiency method of expressing multispecific antibodies in eukaryotic cells. The invention is further drawn to a method of producing immunoglobulin heavy and light chain libraries, particularly using the trimolecular recombination method, for expression in eukaryotic cells. The invention further provides methods of selecting...

20050266411 - Methods for rapid forensic analysis of mitochondrial dna: The present invention provides methods for rapid forensic analysis of mitochondrial DNA by amplification of a segment of mitochondrial DNA containing restriction sites, digesting the mitochondrial DNA segments with restriction enzymes, determining the molecular masses of the restriction fragments and comparing the molecular masses with the molecular masses of theoretical...

20050266486 - Methods for treating obesity by inhibiting expression of obese gene product receptor variant: The present invention relates to a variant form of the receptor for the obese gene product. In particular, the invention relates to methods of detecting this receptor variant in cells and tissues of obese individuals. In addition, it relates to methods of inhibiting or down-regulating expression of this variant in...

20050266410 - Methods of human leukocyte antigen typing by neighboring single nucleotide polymorphism haplotypes: The disclosure relates to novel approaches to mapping the MHC region and provides novel methods of genotyping the HLA loci. A haplotype map of the region and methods of using the map are also disclosed....

20050266418 - Methods, compositions, and kits comprising linker probes for quantifying polynucleotides: The present invention is directed to methods, reagents, kits, and compositions for identifying and quantifying target polynucleotide sequences. A linker probe comprising a 3′ target specific portion, a loop, and a stem is hybridized to a target polynucleotide and extended to form a reaction product that includes a reverse primer...

20050266466 - Microarray-based method for amplifying and detecting nucleic acids during a continuous process: The invention concerns methods, in the context of slide-based evaluation, for efficiency amplifying and detecting nucleic acids during a continuous process. The invention mainly concerns methods which consist in performing a polymerase chain reaction occurs, whereto a competitor element is added at the beginning of the reaction. The invention also...

20050266416 - Molecular nanomotor: A molecular nanomotor useful for translocating polynucleotides. The nanomotor is a multimolecular complex fueled by ATP hydrolysis. One of the motor components is an ATP-binding RNA molecule that participates in ATPase activity....

20050266420 - Multigene predictors of response to chemotherapy: The present invention provides the identification of genes that are expressed in tumors that are responsive to a given therapeutic agent and whose expression (either increased expression or decreased expression) correlates with responsiveness to that therapeutic agent. One or more of the genes of the present invention can be used...

20050266453 - Mutagenesis methods using ribavirin and/or rna replicases: The use of RNA-replicases for introducing mutations into and selecting for improved RNA molecules is described. The use of ribavirin, or a derivative/analogue thereof, in methods for introducing one or more mutations during replication or transcription of a target nucleic acid molecule is also described. These methods can be used...

20050266436 - Mutant b-type dna polymerases exhibiting improved performance in pcr: The present invention relates to thermostable mutants of B-type DNA polymerases comprising a Y-GG/A amino acid motif between the N-terminal 3′-5′-exonuclease domain and the C-terminal polymerase domain whereas the tyrosine of the Y-GG/A amino acid motif is mutated and whereas these mutant DNA polymerases are suitable for PCR....

20050266457 - Mutant receptors and their use in a nuclear receptor-based inducible gene expression system: This invention relates to the field of biotechnology or genetic engineering. Specifically, this invention relates to the field of gene expression. More specifically, this invention relates to novel substitution mutant receptors and their use in a nuclear receptor-based inducible gene expression system and methods of modulating the expression of a...

20050266493 - Novel genes, compositions, kits, and methods for identification, assessment, prevention, and therapy of colon cancer: The invention relates to newly discovered nucleic acid molecules and proteins associated with colon cancer. Compositions, kits, and methods for detecting, characterizing, preventing, and treating human colon cancers are provided....

20050266454 - Novel human kinase proteins and polynucleotides encoding the same: Novel human polynucleotide and polypeptide sequences are disclosed that can be used in therapeutic, diagnostic, and pharmacogenomic applications....

20050266452 - Novel human kinases and polynucleotides encoding the same: Novel human polynucleotide and polypeptide sequences are disclosed that can be used in therapeutic, diagnostic, and pharmacogenomic applications....

20050266483 - Novel method of diagnosing, monitoring, staging, imaging and treating colon cancer: The present invention provides new methods for detecting, diagnosing, monitoring, staging, prognosticating, imaging and treating colon cancer....

20050266423 - Novel nucleic acids and polypeptides: The present invention provides novel nucleic acids, novel polypeptide sequences encoded by these nucleic acids and uses thereof....

20050266441 - Novel oligonucleotide arrays and their use for sorting, isolating, sequencing, and manipulating nucleic acids: The invention provides hyperactive mutant recombinases and hybrid mutant recombinases, and methods for their identification. Also provided are nucleic acids encoding hyperactive mutant recombinases and hybrid recombinases, as well as vectors and host cells. Host cells include eukaryotic cells capable of expressing said recombinases and carrying out site-specific recombination in...

20050266443 - Novel tumor suppressor gene and compositions and methods for making and using the same: The present invention relates to the identification and cloning of ARTS1, a novel tumor suppressor gene. The invention further encompasses isolated proteins encoded by ARTS1, methods of making and using the same, methods of diagnosing the presence of, or prediposition for, a cancer associated with a defective ARTS1 gene or...

20050266484 - Nphs2 gene involved in the steroid-resistant nephrotic syndrome, protein encoded by said gene and diagnostic and therapeutic uses: The invention concerns a novel gene, called NPHS2 gene coding for a protein involved in the cortico-resistant nephrotic syndrome, and diagnostic and therapeutic uses of the novel identified nucleotide sequences and amino acids....

20050266459 - Nucleic acid probes and nucleic acid analog probes: The present invention relates to probes for use in various applications. For example, these probes may be used to detect, quantify, identify, or analyze nucleic acid molecules or other molecules that bind to the probes. The invention also encompasses compositions and kits containing the probes and methods of using the...

20050266431 - Nucleic acids, polypeptides, single nucleotide polymorphisms and methods of use thereof: Disclosed herein is a nucleic acid sequence that encodes a novel polypeptide. Also disclosed is a polypeptide encoded by the nucleic acid sequence, and antibodies, which immunospecifically-bind to the polypeptide, as well as derivatives, variants, mutants, or fragments of the aforementioned polypeptide, polynucleotide, or antibody. The invention further discloses therapeutic,...

20050266481 - Polymorphisms in the region of the human hemochromatosis gene: Polymorphic sites in the region surrounding the HH gene are provided. These polymorphisms are useful as surrogate markers in diagnostic assays for hemochromatosis....

20050266440 - Pufa polyketide synthase systems and uses thereof: The invention generally relates to polyunsaturated fatty acid (PUFA) polyketide synthase (PKS) systems isolated from or derived from non-bacterial organisms, to homologues thereof, to isolated nucleic acid molecules and recombinant nucleic acid molecules encoding biologically active domains of such a PUFA PKS system, to genetically modified organisms comprising PUFA PKS...

20050266428 - Purification methods: (iii) at least one linker of the formula (I) wherein R1 and R2 may be the same or different and are independently selected from the group consisting of: H, OH, C1-C6 alkyl, C2-C6 alkenyl, halogen, C1-C6 alkoxy, C2-C6 alkenyloxy and aryloxy, or R1 or R2 when taken together with an...

20050266445 - Purified mammalian ctla-8 antigens and related reagents: CTLA-8 antigen from a mammal, reagents related thereto including purified proteins, specific antibodies, and nucleic acids encoding said antigen. Methods of using said reagents and diagnostic kits are also provided....

20050266477 - Reagents and methods useful for detecting diseases of the gastrointestinal tract: A set of contiguous and partially overlapping cDNA sequences and polypeptides encoded thereby, designated as CS194 and transcribed from GI tract tissue, is described. These sequences are useful for the detecting, diagnosing, staging, monitoring, prognosticating, preventing or treating, or determining the predisposition of an individual to diseases and conditions of...

20050266424 - Real-time sequence determination: A sequencing methodology is disclosed that allows a single DNA or RNA molecule or portion thereof to be sequenced directly and in substantially real time. The methodology involves engineering a polymerase and/or dNTPs with atomic and/or molecular tags that have a detectable property that is monitored by a detection system....

20050266473 - Reconstituted histone methyltransferase complex and methods of identifying modulators thereof: The present invention provides a reconstituted complex including EED, EZH2 and SUZ12 wherein the reconstituted complex has histone methyltransferase (HMTase) activity for lysine 27 of histone H3 (H3-K27). The reconstituted complex may further include RbAp48, AEBP2 or both. Also disclosed are methods of producing the reconstituted complex, methods of identifying...

20050266482 - Regulation of human cyslt2-like gpcr protein: Reagents which regulate human CysLT2-like GPCR protein and reagents which bind to human CysLT2-like GPCR gene products can play a role in preventing, ameliorating, or correcting dysfunctions or diseases including, but not limited to peripheral and central nervous system disease, asthma and cardiovascular disease....

20050266491 - Renilla reniformis fluorescent proteins, nucleic acids encoding the fluorescent and the use thereof in diagnostics, high throughput screening and novelty items: Isolated and purified nucleic acids encoding green fluorescent proteins from Renilla reniformis and the green fluorescent protein encoded thereby are also provided. Mutants of the nucleic acid molecules and the modified encoded proteins are also provided. Compositions and combinations comprising the green fluorescent proteins and/or the luciferase are further provided....

20050266461 - Secreted and transmembrane polypeptides and nucleic acids encoding the same: The present invention is directed to novel polypeptides and to nucleic acid molecules encoding those polypeptides. Also provided herein are vectors and host cells comprising those nucleic acid sequences, chimeric polypeptide molecules comprising the polypeptides of the present invention fused to heterologous polypeptide sequences, antibodies which bind to the polypeptides...

20050266490 - Secreted and transmembrane polypeptides and nucleic acids encoding the same: The present invention is directed to novel polypeptides and to nucleic acid molecules encoding those polypeptides. Also provided herein are vectors and host cells comprising those nucleic acid sequences, chimeric polypeptide molecules comprising the polypeptides of the present invention fused to heterologous polypeptide sequences, antibodies which bind to the polypeptides...

20050266494 - System and method for computer network ordering of biological testing: A system and method for networked ordering biological tests is provided including communicating biological test sample information and test information over the Internet....

20050266439 - Thermostable rna ligase from thermus phage: An RNA ligase derived from bacteriophage TS2126 which infects Thermus scotoductus, nucleic acids comprising nucleotide sequences of open reading frame (ORF) and polypeptides encoded by the nucleic acids, are described....

20050266444 - Use of representations of dna for genetic analysis: It is an object of the present invention to provide a solution to problems associated with the use of microarray technology for the analysis DNA. The present invention provides compositions and methods for the use of simple and compound representations of DNA in microarray technology. The present invention is also...

20050266435 - Vr1 receptors and uses thereof: The present invention relates to nucleic and amino acid sequences encoding vanilloid receptors which are vanilloid-insensitive. The present invention provides mutant vanilloid receptors which are insensitive to vanilloid, but which are capable of responding to low pH, heat and other receptor modulators. The invention particularly provides mutant vanilloid insensitive human...

20050266496 - Heterocyclic compounds, combinatorial libraries thereof and methods of selecting drug leads: Heterocyclic compounds having a relatively flexible backbone are used to create combinatorial libraries that permit screening for lead compounds and selection of drug candidates for a variety of uses in human and veterinary medicine as well as in agriculture. The compounds of the library generally differ in ring size and...

20050266499 - Method and apparatus for testing for presence or absence of select biological substances: A fluid flow test device comprising: a chromatography path of aqueous wicking material on which is deposited at an upstream site at a selected delivery time an aqueous biological sample to be tested for the presence or absence of a target material; a capture material bound to the wicking material...

20050266495 - Method and program for library generation: A library generation device determines a characteristic approximation function based on a characteristic extraction table to calculate characteristic data corresponding to a client's conditions in accordance with the characteristic approximation function. A library for verification including the calculated characteristic data and format information of a reference library is generated in...

20050266498 - Method for screening biomolecule activity regulator: A method for screening a low molecular weight substance which binds to a specific site of a biomolecule and may be usable as a starting material in drug development. This method comprises screening a substance that interacts with a specific region of a biomolecule having an activity, to regulate the...

20050266497 - Method of assessing the risk of atherosclerosis: A method for aiding in the determination of whether a living or deceased human is afflicted with or will likely have atherosclerosis respectfully, the method comprising determining the level of SPRR3 protein in a intimae or body fluid sample by using molecular localization analysis technique comparing the determined level of...

20050266500 - Real time detection of intermolecular interaction: Intermolecular binding can be detected by formation of a “paratope” which results in an immediate generation of a signal. The substances to be tested for interaction are bound to demitopes, wherein said demitopes are components of a paratope which binds a reporter which provides said signal when bound. Known interactions...

20050266504 - Methods for diagnosing and treating alzheimers disease and parkinson's disease: The present invention relates to a method for diagnosing Alzheimer's disease and Parkinson's disease in a subject by analyzing the expression of Semaphorin 3 and downstream effectors. It also provides a method for identifying a substance useful in the prevention or treatment of Alzheimer's disease and Parkinson's disease, and a...

20050266502 - Methods, compositions and compound assays for inhibiting amyloid-beta protein production: A method for identifying compounds that inhibit amyloid-beta precursor protein processing in cells, comprising contacting a test compound with a GPCR polypeptide, or fragment thereof, and measuring a compound-GPCR property related to the production of amyloid-beta peptide. Cellular assays of the method measure indicators including second messenger and/or amyloid beta...

20050266501 - Reagent for detecting risk factor of alzheimer's disease, detection kit therefor and method of detecting risk factor of alzheimer's disease using the same: A reagent for detecting a risk factor of Alzheimer's disease which contain, as the active ingredients, an antibody specific to apolipoprotein E4 and an antibody against all of apolipoprotein E2, apolipoprotein E3 and apolipoprotein E4; a detection method; and a detection kit. Thus, apolipoprotein E4 genotype can be economically and...

20050266503 - Simultaneous assay of target and target-drug binding: Whole cell, simultaneous target and drug-target assay using differentially labeled antibodies and flow cytometry. First antibody binds to total target and second antibody binds to the drug binding site of the target, thus drug binding will competitively inhibit the second antibody allowing for a competitive inhibition assay of drug-target binding....

20050266505 - Proteomic analysis: Activity-based compositions for analyzing metalloproteases are disclosed, where the compositions include a chemical compound including a hydroxamate moiety and a benzophenone moiety. Methods for synthesizing these compounds are also disclosed, as well as methods of using them for determining the bioactivity of a compositions comprising active compounds toward a metalloproteases...

20050266507 - Antibody pair screening methods: The invention provides methods for identifying antibody preparations that can form a pair of antibodies that optimally detect a target antigen, for example, in a sandwich immunoassay. These methods provide high affinity and epitope-specific antibodies....

20050266506 - Method for diagnosing or monitoring carbohydrate metabolism disorders: This invention provides a method for diagnosing geriatric diseases associated with insulin resistance, such as type II diabetes, at an early stage and a method for monitoring the therapeutic effects of agents for type II diabetes by quantitatively assaying GBP28 with the use of a monoclonal antibody that can assay...

20050266508 - Detection of potassium ions using ion-sensitive enzymes: The invention relates generally to the field of potassium ion detection. In particular, the invention provides methods and kits for assaying for potassium ions in a sample, using inter alia, a potassium-dependent urea amidolyase (UAL)....

20050266509 - Interaction of nmda receptor with protein tyrosine phosphatase: The present invention relates to the identification of a binding between NMDA receptor (NMDA-R) subunits and the protein tyrosine phosphatase PTPMEG. The present invention provides methods for screening a PTP agonist or antagonist that modulates NMDA-R signaling. The present invention also provides methods and compositions for treatment of disorders mediated...

20050266510 - T cell anergy is reversed by active ras and regulated by diacylglycerol kinase: T cell anergy has been correlated with defective Ras signaling. However, neither a causal relationship nor the mechanism of Ras hypoactivation have been established. Using adenoviral transduction of CAR Tg T cells to enable genetic manipulation in nonproliferating cells, we show that Ras61L restores IL-2 production and MAP kinase signaling...

20050266511 - High throughput sarcomeric assay: The present invention provides high throughput screening systems for identifying compounds that modulate the biological activity of a biochemically functional sarcomere. The method can be performed in plurality simultaneously with fluorescence or absorbance readouts....

20050266512 - Detection of proteases and screening for protease inhibitors: The present disclosure provides a method for detecting a protease, which is simple, sensitive, and capable of high throughput. The method detects a protease in a sample by measuring lysis of a liposome due to activation of a modified or inactive channel-forming agent. Also disclosed is a method for screening...

20050266513 - Method for identifying compounds that inhibit the activity of myc: Method for identifying compounds useful for the therapy of cancers in which the activity of Myc is causally involved. The method is based on measuring the activity of the E3 ligase HectH9, which is required for ubiquitination and activation of Myc....

20050266515 - Glyceraldehyde 3-phosphate dehydrogenase-s (gapdhs), a glycolytic anzyme expressed only in male germ cells, is a target for male contraception: Methods for identifying modulators of a male germ cell-specific glyceraldehyde 3-phosphate dehydrogenase (GAPDHS) are disclosed. Also disclosed are methods for screening potential modulators for an ability to modulate biological functions of a GAPDHS polypeptide....

20050266514 - Procedure for anti-limulus-factors-independent determination of lipopolysaccharide- and/or lipid-a- and/or glucan- reactivity, suitable test system for this as well as its usage: Described is an anti-Limulus-factors-independent procedure to measure the lipopolysaccharide (LPS)- and/or lipid-A and/or glucan-reactivity in biologic liquids with the aid of a modified Limulus-test. Anti-Limulus-factors-independence is obtained by strongly-oxidative treatment of the sample....

20050266516 - System for rapid analysis of microbiological materials in liquid samples: A system for the rapid analysis of microbiological parameters includes a specimen container for containing a liquid sample, a housing having an enclosable chamber shaped for receiving the specimen container, an incubating system mounted within the housing for incubating microbiological materials within the liquid sample, and a spectrophotometer system mounted...

20050266517 - Transformant containing transaldolase gene: The present invention relates to a novel transaldolase gene, and to a polypeptide encoded by the gene, a recombinant DNA obtained by ligating the gene, a transformant carrying the recombinant DNA, and a process for producing the polypeptide, aromatic amino acids, aromatic vitamins, L-histidine, riboflavin, nucleic acids, nucleic acid-associated substances,...

20050266518 - Isoprenoid production: Isolated polynucleotides encoding polypeptides having the activity of enzymes in the mevalonate pathway are provided. These sequences are useful for recombinantly producing isoprenoid compounds, such as carotenoids, in particular zeaxanthin. Expression vectors, cultured cells, and methods of making isoprenoid compounds are also provided....

20050266524 - Beta integrin gene and protein: Recombinant materials for the production of β integrin protein characteristic of Manduca sexta are useful for screening pesticides for this moth....

20050266528 - Composition exhibiting a von willebrand factor (vwf) protease activity comprising a polypeptide chain with the amino acid sequence aaggilhlellv: The invention relates to vWF cleaving entities having a molecular weight of 180 kD, 170 kD, 160 kD, 120 kD or 110 kD and an N-terminal amino acid sequence of AAGGILHLELLV, vWF cleaving complexes and methods for their production....

20050266525 - Expression control sequence: An expression control sequence which controls expression of a target gene linked downstream of the expression control sequence depending on an intracellular concentration of an amino acid, wherein in a bacterial cell which harbors a DNA construct comprising the expression control sequence, a promoter linked upstream of the expression control...

20050266526 - Glycoprotein synthesis: Methods for making glycoproteins, both in vitro and in vivo, are provided. One method involves incorporating an unnatural amino acid into a protein and attaching one or more saccharide moieties to the unnatural amino acid. Another method involves incorporating an unnatural amino acid that includes a saccharide moiety into a...

20050266523 - Glycorandomization and the production of novel erythronolide and coumarin analogs: The present invention provides combinatorial methods for rapidly generating a diverse library of glycorandomized structures, comprising incubating one or more aglycons and a pool of NDP-sugars in the presence of a glycosyltransferase. The glycosyltransferase may be one that is associated with or involved in production of natural secondary metabolites, or...

20050266522 - Human amine receptor: A Human amine receptor polypeptide and DNA (RNA) encoding such polypeptide and a procedure for producing such polypeptide by recombinant techniques is disclosed. Also provided are methods for detecting compounds which bind to and activate and bind to and inhibit such polypeptide and the use of compounds for treating diseases...

20050266527 - Human g-protein receptor hibef51: Human G-protein Coupled receptor (HIBEF51) polypeptides and DNA (RNA) encoding such polypeptides and a procedure for producing such polypeptides by recombinant techniques is disclosed. Also disclosed are methods for utilizing such polypeptides for identifying antagonists and agonists to such polypeptides and methods of using the agonists and antagonists therapeutically to...

20050266519 - Methods and compositions for modulating and detecting activin dimer and dimer formation: Methods and compositions for modulating activin dimer formation, such as the formation of activin dimers formed by the dimerisation of activin subunits βA, βB, βC, βD, or βE, or combinations thereof, are provided. The invention also relates to methods and compositions for detecting an activin monomer or dimer using, for...

20050266529 - Novel human g-protein coupled receptor: The present invention relates to novel identified polynucleotides, polypeptides encoded by them and to the use of such polynucleotides and polypeptides, and to their production. More particularly, the polynucleotides and polypeptides of the present invention relate to the G-protein coupled receptor family, referred to as IGS4-family. The invention also relates...

20050266520 - Process for the synthesis of peptides: A process is provided for the solid-phase synthesis of a peptide amide which comprises attaching a α-nitrogen protect Cα-carboxamide amino acid to a solid support by its side chain, removing the α-nitrogen protecting group, assembling a peptide chain on said α-nitrogen and then cleaving the assembled peptide amide from the...

20050266521 - Production method for iturin a and its homologues: (1) A method for producing iturin A and its homologues by cultivating a Bacillus microbe that produces iturin A and its homologue in a medium containing 2 mass % or more of soybean pulverisate or its extract as a nitrogen source to allow the microbe to accumulate iturin A and...

20050266530 - Cynomolgus prostate specific antigen: Isolated polynucleotides encoding Cynomolgus monkey prostate specific antigen and polypeptides obtainable from the polynucleotides and uses are disclosed....

20050266531 - Compositions and methods for inhibiting liver stellate cell growth: The present invention provides compositions and methods for selectively inhibiting the proliferation of stellate cells, which are important for the development of liver fibrosis upon liver injury. The invention describes conditioned media from immortalized hepatocytes as containing a death factor that induces apoptosis of activated liver stellate cells. This pro-apoptotic...

20050266532 - Albumin fusion proteins: The present invention encompasses albumin fusion proteins. Nucleic acid molecules encoding the albumin fusion proteins of the invention are also encompassed by the invention, as are vectors containing these nucleic acids, host cells transformed with these nucleic acids vectors, and methods of making the albumin fusion proteins of the invention...

20050266533 - Albumin fusion proteins: The present invention encompasses albumin fusion proteins. Nucleic acid molecules encoding the albumin fusion proteins of the invention are also encompassed by the invention, as are vectors containing these nucleic acids, host cells transformed with these nucleic acids vectors, and methods of making the albumin fusion proteins of the invention...

20050266534 - Nucleotide sequences which code for the csta gene: The invention relates to an isolated polynucleotide having a polynucleotide sequence which codes for the cstA gene, and a host-vector system having a coryneform host bacterium in which the cstA gene is present in attenuated form and a vector which carries at least the cstA gene according to SEQ ID...

20050266535 - Nucleotide sequences which code for the mete gene: e

20050266536 - Process for the fermentative preparation of l-amino acids using coryneform bacteria: A process for the preparation of L-amino acids, in which the following steps are carried out: (a) fermentation of the coryneform bacteria which produce the desired L-amino acid and in which at least the gene which codes for trehalose phosphatase and/or the gene which codes for maltooligosyl-trehalose synthase and/or the...

20050266538 - Process for producing arachidonic acid-containing lipids and dihomo-gamma-linolenic acid containing lipids: A process for producing lipids containing arachidonic acid comprising culturing a microorganism in which ω3 desaturase activity has been decreased or is lacking at a temperature lower than the optimum growth temperature from the start of culturing or after culturing at the optimum growth temperature, said microorganism being obtained by...

20050266537 - Process for producing poly-unsaturated fatty acids by oleaginous yeasts: The present invention is directed to a process of producing novel fatty acids in oleaginous yeast by producing oleaginous yeast by introducing into the yeast genes coding for enzymes selected from the group consisting of D5-desaturase, D6-desaturase, D12-desaturase, D15-desaturase and elongase; and culturing the yeast in the medium containing high...

20050266539 - Apparatus for the production of alcohol: The aim of the invention is to produce alcohol from plant-based raw materials while reducing energy consumption. Said aim is achieved by separating the husk protion of cereals during grinding and feed said husk portion to a dryer (21) which dries distiller's wash as a carrier medium for distiller's wash....

20050266540 - Ethanol production from biological wastes: The invention provides methods, apparatuses, and kits for producing ethanol and other alcohols. The methods involve fermenting organic material in a fermentation mixture to a biogas comprising methane; converting at least a portion of the biogas to synthesis gas comprising CO and H2; and contacting at least a portion of...

20050266541 - Methods and compositions for evolving microbial hydrogen production: The invention provides methods and compositions for engineering cells to generate large amounts of hydrogen. Genes that are involved in hydrogen production pathways and genes that are upregulated when cells are exposed to conditions conducive to the generation of hydrogen are mutagenized according to disclosed protocols. Microbes containing nucleic acid...

20050266542 - Methods for refining concentrated enzyme solutions: Concentrated enzyme solutions are refined by a process comprising the steps of: (A) providing a concentrated enzyme solution comprised of insoluble solids; (B) separating the insoluble solids to produce a solids-free supernatant solution and (C) contacting the supernatant solution with a strongly basic anion exchanger carried out with a bed...

20050266543 - Heterologous expression of an aspergillus kawachi acid-stable alpha amylase and applications in granular starch hydrolysis: The present invention relates to a granular starch hydrolyzing enzyme composition comprising an acid stable alpha amylase (asAA) having granular starch hydrolyzing activity. The invention also relates to a one-step method for producing an alcohol which comprises contacting a granular starch substrate with an acid-stable alpha amylase (asAA) having granular...

20050266544 - Methods and compositions for increasing viral vector production in packaging cell lines: Methods and compositions are disclosed to increase viral titer of vector producing cell lines and to reduce potential for re-infection by inhibiting deactivation of helper virus. According to the invention, methylation of helper virus and concomitant helper virus inactivation is directly correlated with increased super-infection and decreased vector production. Novel...

20050266545 - Lactic acid bacteria with immunoregualting activites: The invention provides novel lactic acid bacteria having immunoregulating activities and usable in food and drinks, medicaments, and other applications. Specifically, the invention provides novel lactic acid bacteria separated from “Shibazuke,” one kind of traditional Kyoto pickles, and having immunoregulating activities. The lactic acid bacteria of the present invention belong...

20050266546 - Biodegradable polymers: The invention relates to methods of solubilizing and recycling polymers using irradiation, wherein the polymers comprise photoreactive moieties. These polymers have many applications including use in disposable consumer products such as beverage bottles, eating utensils and diapers....

20050266547 - Cell culture chamber for a cell culture system: A cell culture chamber (20) for a closed cell culture system serving to continuously supply different cells with liquid nutrient media, growth factors, gases or the like essentially consists of a membrane plate (1) with a membrane (2) for accommodating at least one cell culture and with a number of...

20050266548 - Biocatalyst chamber encapsulation system for bioremediation and fermentation with improved rotor: The invention comprises novel culture methods and devices in which biocatalysts are substantially immobilized contained, suspended and/or incubated in a chamber. At least one injection element provides a fluid flow to a perimeter of the chamber and the fluid force of the fluids flowing into the chamber works with the...

20050266549 - Molecular switch for regulating mammalian gene expression: Expression vectors are disclosed that are comprised of (a) one or more silencer elements and conditionally inducible elements to form silencer-inducible regions and (b) promoters in operative linkage upstream of at least one expressed region. The expression vector thereby regulates expression of at least one downstream region by conditional silencing...

20050266550 - Tc-83-derived alphavirus vectors, particles and methods: The present disclosure provides TC-83 VEE-derived replicons, alphaviral replicon particles and immunogenic compositions containing TC-83 alphaviral replicon particles which direct the expression of at least one antigen when introduced into a suitable host cell. The TC-83 VEE-derived ARPs described herein are improved in that they are subject to a lower...

20050266551 - Method for the controlled release of calcium from intracellular calcium stores of oocytes: A method for the controlled release of calcium from intracellular calcium stores of oocytes, by i) establishing an optimum profile for release of calcium from intracellular calcium stores of oocytes, and ii) applying the following cycles to the oocytes: (A) a pulse perfusion cycle which comprises a step of perfusing...

20050266552 - Reagents and methods for identification of rnai pathway genes and chemical modulators of rnai: The present invention provides reagents such as cells, cell lines, and vectors, that can be used to identify mammalian genes whose expression products (RNA or protein) play a role in RNA interference (RNAi) and/or to identify chemical modulators of RNAi, or for other purposes. The invention further provides a variety...

20050266553 - Methods of regulating differentiation in stem cells: The present invention provides methods, media and compositions capable of modulating the differentiation of stem cells. Applicants have discovered that agonists of lysophospholipid receptors and ligands of class III tyrosine kinase receptors are useful in preventing the spontaneous differentiation of stem cells. The ligands and agonists may be used alone,...

20050266554 - Pdx1 expressing endoderm: Disclosed herein are cell cultures comprising PDX1-positive endoderm cells and methods of producing the same. Also disclosed herein are cell populations comprising substantially purified PDX1-positive endoderm cells as well as methods for enriching, isolating and purifying PDX1-positive endoderm cells from other cell types. Methods of identifying differentiation factors capable of...

20050266555 - Progenitor cells, methods and uses related thereto: The present invention relates to a substantially pure population of viable pancreatic progenitor cells, and methods for isolating such cells. The present invention further concerns certain therapeutic uses for such progenitor cells, and their progeny....

20050266556 - Isolation, expansion and use of clonogenic endothelial progenitor cells: A hierarchy of endothelial colony forming cells (EPCs) was identified from mammalian cord blood, umbilical vein and aorta. A newly isolated cell named high proliferative potential—endothelial colony forming cell (HPP-ECFC) was isolated and characterized. Single cell assays were developed that test the proliferative and clonogenic potential of endothelial cells derived...

20050266557 - Method of expanding cord blood cells: Based on previous evidence suggesting positive effects of fever on in vivo hematopoiesis, the effect of hyperthermia on the expansion and differentiation of megakaryocytes (MKs) in ex vivo cultures of CB CD34-enriched cells has now been tested. Cells were cultured at 37° C. or 39° C. for 14 days in...

20050266558 - Compositions isolated from forage grasses and methods for their use: Isolated polynucleotides encoding polypeptides that regulate flowering are provided, together with expression vectors and host cells comprising such isolated polynucleotides. Methods for the use of such polynucleotides and polypeptides are also provided....

20050266560 - Plant chromosome compositions and methods: The present invention provides for the identification and cloning of functional plant centromeres in Arabidopsis. This will permit construction of stably inherited minichromosomes which can serve as vectors for the construction of transgenic plant and animal cells. In addition, information on the structure and function of these regions will prove...

20050266559 - Shade responsive promoter, promoter control elements, and combinations, and uses thereof: The present invention is directed to promoter sequences and promoter control elements, polynucleotide constructs comprising the promoters and control elements, and methods of identifying the promoters, control elements, or fragments thereof. The invention further relates to the use of the present promoters or promoter control elements to modulate transcript levels....

20050266563 - Methods and compositions relating to neuronal cell and tissue differentiation: The invention relates to methods for isolating and purifying specific types of neurons, such as cortical or other projection neurons including corticospinal motor neurons, subcerebral projection neurons, and callosal projection neurons. The invention also relates to genes that are specific for particular neuronal subtypes, and the use of such genes...

20050266562 - Myeloid cell promoter and constructs containing same: The present invention provides novel myeloid cell specific promoters, and cis-acting elements that influence the activity of a myeloid cell specific promoters, as well as promoter-heterologous gene constructs and transfected myeloid cells which include the novel promoters and/or the novel cis-acting elements. The present invention also provides a method of...

20050266561 - Use of interfering rna in the production of transgenic animals: The invention provides cells and animals, as well as methods of producing cells and animals, that express at least one interfering RNA molecule to regulate the expression of a specific gene or family of genes. The invention further provides novel iRNA molecules, as well as DNA templates for producing iRNA...

20050266567 - Methods for generating high titer helper-free preparations of released recombinant aav vectors: This invention provides methods and compositions for producing high titer, substantially purified preparations of recombinant adeno-associated virus (AAV) that can be used as vectors for gene delivery. At the onset of vector production, AAV producer cells of this invention typically comprise one or more AAV packaging genes, an AAV vector...

20050266565 - Novel lentiviral vectors for site-specific gene insertion: Murine leukemia virus (MLV) and lentivirus vectors have been used previously to deliver genes to hematopoietic stem cells (HSCs) in human gene therapy trials. However, these vectors integrate randomly into the host genome, leading to disruption or inactivation of vital host genes. The present invention discloses a novel lentiviral vector...

20050266566 - Recombinant sendai virus: A method for regenerating Sendai virus particles by transfecting the Sendai virus genome to a host expressing all genes for the initial viral replication has been developed, enabling the genetic manipulation of Sendai virus and effective utilization of said virus as the vector....

20050266564 - Tetracycline-regulated gene expression in hsv-1 vectors: The present invention is directed to HSV-1 vectors which rely on the tetracycline repressor and operator as a means for regulating expression. The vectors utilize VP-16 responsive promoters of HSV to control expression of the tetracycline repressor. The vectors are of particular interest as vehicles for recombinantly expressing genes in...

20050266568 - Molecule-releasing apparatus and molecule-releasing medhod: A molecule-releasing apparatus includes a releasing unit configured to release molecules, wherein the releasing unit comprises at least one conductive member and releases molecules electrically interacting with the conductive member to which a potential is applied, from the conductive member by changing the potential so as to remove the interaction....

20050266569 - Regulator/promoter for tunable gene expression and metabolite sensing: A method for the facile and inexpensive inducible expression of heterologous genes has been discovered. The yhcS regulator gene has been found to be inducible by aromatic carboxylic acids and to alter the expression of operons in the LysR gene family, including the yhcRQP operon, common in enteric bacteria. Heterologous...