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  Casein hydrolyzate, process for producing the same and use thereofUSPTO Application #: 20070299014Title: Casein hydrolyzate, process for producing the same and use thereof Abstract: The present invention relates to a casein hydrolysate containing free amino acids and in vivo indigestible peptides having minimally suppressed in vivo enzymatic digestibility, and expected to express functions, such as hypotensive effect, in living organism, and to a method for preparing such a hydrolysate, and use thereof. The casein hydrolysate of the present invention contains free amino acids and peptides, such as in vivo indigestible peptides including Xaa-Pro and Xaa-Pro-Pro, obtained by hydrolyzing animal milk casein to have an average chain length of not longer than 2.1 in terms of the number of amino acid residues, and has ACE inhibitory activity or hypotensive effect. (end of abstract)
Agent: Darby & Darby P.C. - New York, NY, US Inventors: Naoyuki Yamamoto, Seiichi Mizuno, Shingo Nishimura, Hideo Nishimura, Takanobu Gotou, Keiichi Matsuura, Tadashi Shinoda USPTO Applicaton #: 20070299014 - Class: 514018000 (USPTO) Related Patent Categories: Drug, Bio-affecting And Body Treating Compositions, Designated Organic Active Ingredient Containing (doai), Peptide Containing (e.g., Protein, Peptones, Fibrinogen, Etc.) Doai, Cyclopeptides, 3 Or 4 Peptide Repeating Units In Known Peptide Chain The Patent Description & Claims data below is from USPTO Patent Application 20070299014. Brief Patent Description - Full Patent Description - Patent Application Claims
FIELD OF ART [0001] The present invention relates to casein hydrolysate which is obtained by hydrolysis of animal milk casein, is expected to exhibit various functions including angiotensin converting enzyme inhibitory activity and hypotensive effect, and is useful for various food and medicine, as well as to a method for preparing such casein hydrolysate, and use thereof. BACKGROUND ART [0002] A variety of peptides have been reported having various functions, such as hypotensive effect, anti-bacterial activity, calcium solubilizing effect, and immunomodulating effect, and these peptides are in use in food and medicine. [0003] For example, hypotensive peptides have been proposed, many of which have angiotensin converting enzyme (abbreviated as ACE hereinbelow) inhibitory activity. ACE converts a precursor, angiotensin I, to angiotensin II having vasoconstrictive activity in living organism, to thereby raise the blood pressure. Thus peptides having ACE inhibitory activity are expected to exhibit hypotensive effect by inhibiting ACE to suppress production of angiotensin II in living organism. In the development of hypotensive peptides, researches are usually directed first to peptides having ACE inhibitory activity, so that hypotensive effect of the proposed peptides are mainly indicated by ACE inhibitory effect as reference index. A great number of agents having hypotensive effect evaluated by the ACE inhibitory activity as reference index, have been proposed to date, and are in use for prevention and treatment of hypertension. [0004] In production of functional peptides, such as peptides having hypotensive effect evaluated by the ACE inhibitory effect, in particular, in production of functional peptides by digestion of food protein with enzymes, complicated steps are usually required after the enzymatic digestion, such as concentration, purification, and isolation of the digested products, for improving expression of the functional effects. [0005] As peptides that are absorbed through the alimentary canal into blood to express their functions in living organism, in vivo indigestible peptides are expected to be advantageous that have high absorbability and digestion resistance against various digestive enzymes in living organism. However, it is not known in detail which peptides contribute to enhancement of the in vivo digestion resistance. Thus development of enzymatic digestion products for food or medicine and methods for producing such products are desired, which products have high in vivo digestion resistance, and can effectively express their desired functions without undergoing complicated processes such as concentration, purification, or isolation after the enzymatic digestion. [0006] For example, Patent Publication 1 discloses a method for producing a low molecular weight peptide mixture mainly composed of dipeptides and tripeptides, having an average chain length of not longer than 3, and having excellent intestinal absorbability. The peptide mixture is prepared from soybean protein by simultaneous or sequential action of two or more enzymes having endoprotease activity but substantially no exoprotease activity, with not higher than 5% free amino acids being generated. Patent Publication 2 discloses functional food utilizing soybean protein digestion products and method for producing the same. The digestion products contain as active ingredients a dipeptide and tripeptides consisting of Ala-Tyr, Gly-Tyr-Tyr, Ala-Asp-Phe, and Ser-Asp-Phe, prepared by digesting heat denatured soybean protein with enzymes such as proteases derived from Aspergillus oryzae. The digestion products preferably have an average peptide chain length of 2 to 4, and contain 20 to 30 wt % free amino acids. [0007] However, these enzymatic digestion products of soybean protein have components that are quite different from those of enzymatic digestion products of animal milk casein. Thus the above patent publications suggest nothing about methods for preparing, from animal milk casein as a staring material, casein digestion products and casein hydrolysate that have a high concentration of active ingredients and excellent absorbability in living organism, and may be used without necessarily undergoing complicated processes such as concentration, purification, and isolation. [0008] On the other hand, Patent Publications 3 and 4 propose methods for preparing peptides having various functions by digesting animal milk casein with enzymes such as proteases and peptidases, and particular functional peptides obtained by the methods. [0009] The enzymatic digestion products disclosed in these publications are, however, for obtaining particular peptides as active ingredients. Thus there is no teaching in these publications as to hydrolysis of animal milk casein to have an average chain length of not longer than 2.1, specific process of the hydrolysis, and effectiveness of the casein hydrolysate having the particular average chain length. [0010] Patent Publication 1: JP-5-252979-A [0011] Patent Publication 2: JP-2003-210138-A [0012] Patent Publication 3: JP-6-128287-A [0013] Patent Publication 4: JP-2001-136995-A SUMMARY OF THE INVENTION [0014] It is an object of the present invention to provide a casein hydrolysate containing in vivo indigestible peptides and free amino acids having minimized in vivo enzymatic digestibility, and expected to express functions, such as hypotensive effect, in living organism. [0015] It is another object of the present invention to provide a method for preparing the casein hydrolysate mentioned above, which allows easy and efficient production of the above casein hydrolysate without necessarily involving complicated processes. [0016] It is yet another object of the present invention to provide an agent having ACE inhibitory activity or hypotensive effect that contains in vivo indigestible peptides and free amino acids, is expected to have excellent hypotensive effect in living organism, and is useful for various functional food or medicine. [0017] The present inventors have made intensive researches for achieving the above objects, to find out that, by hydrolyzing animal milk casein to have an average chain length of not longer than 2.1 in terms of the number of amino acid residues, a casein hydrolysate containing free amino acids and low molecular weight peptides, such as tripeptides and dipeptides, may be obtained, wherein free amino acids and in vivo indigestible peptide molecules having a residue Pro at the carboxyl terminal are efficiently formed. [0018] The present inventors have also found out that the in vivo indigestible peptides having a residue Pro at the carboxyl terminal are expected to have high digestion resistance against in vivo peptidases, so that it is quite possible for such indigestible peptides to fully exhibit their functions in living organism, and that the casein hydrolysate has a high content of peptides having good in vivo absorbability, such as dipeptides and tripeptides, so that the casein hydrolysate is capable of fully exhibiting various functions, such as hypotensive effect, in living organism, to thereby completing the present invention. [0019] Further, the present inventors have made researches for enzymes that efficiently produce the case in hydrolysate of the present invention from various known enzymes to find out that a particular class of enzymes is particularly capable of efficiently producing the casein hydrolysate of the present invention. [0020] According to the present invention, there is provided a casein hydrolysate comprising free amino acids and peptides obtained by hydrolyzing animal milk casein to have an average chain length of not longer than 2.1 in terms of the number of amino acid residues. More specifically, the present invention provides the casein hydrolysate mentioned above wherein the peptides comprises in vivo indigestible peptides consisting of dipeptides having a sequence Xaa-Pro and tripeptides having a sequence Xaa-Pro-Pro, and wherein a content of the dipeptides having a sequence Xaa-Pro is not lower than 5 wt % of a total amount of the peptides and the free amino acids in the hydrolysate, and a content of the tripeptides having a sequence Xaa-Pro-Pro is not lower than 1 wt % of a total amount of the peptides and the free amino acids in the hydrolysate. As used herein, Xaa may be any amino acid. [0021] According to the present invention, there is also provided a method for preparing the casein hydrolysate mentioned above comprising the step of (A) hydrolyzing animal milk casein to have an average chain length of not longer than 2.1 with a group of enzymes capable of digesting animal milk casein into a casein hydrolysate having an average chain length of not longer than 2.1 in terms of the number of amino acid residues. Continue reading... Full patent description for Casein hydrolyzate, process for producing the same and use thereof Brief Patent Description - Full Patent Description - Patent Application Claims Click on the above for other options relating to this Casein hydrolyzate, process for producing the same and use thereof patent application. ###  How KEYWORD MONITOR works... a FREE service from FreshPatents1. Sign up (takes 30 seconds). 2. Fill in the keywords to be monitored. 3. Each week you receive an email with patent applications related to your keywords. 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