Bacterial biosensors

Title: Bacterial biosensors

Related Patent Categories: Chemistry: Molecular Biology And Microbiology, Measuring Or Testing Process Involving Enzymes Or Micro-organisms; Composition Or Test Strip Therefore; Processes Of Forming Such Composition Or Test Strip, Involving Antigen-antibody Binding, Specific Binding Protein Assay Or Specific Ligand-receptor Binding Assay, Involving A Micro-organism Or Cell Membrane Bound Antigen Or Cell Membrane Bound Receptor Or Cell Membrane Bound Antibody Or Microbial Lysate, Bacteria Or Actinomycetales

Brief Patent Description - Full Patent Description - Patent Claims

The Patent Description & Claims data below is from USPTO Patent Application 20050272105, Bacterial biosensors.

What is claimed is:

1. A bacterial biosensor comprising: a) a substrate having a layer of polysiloxane affixed thereto; and b) bacterial specific selector molecules, adsorbed onto the polysiloxane layer.

2. The bacterial biosensor of claim 1 wherein the bacterial specific selector molecules are peptides.

3. The bacterial biosensor of claim 2 wherein the substrate is an electrode including Indium-Tin-Oxide (ITO) glass.

4. The bacterial biosensor of claim 3 wherein the ITO glass is treated with NaOH prior to affixing the polysiloxane thereto.

5. The bacterial biosensor of claim 1 wherein the bacterial specific selector molecules are selected from the group consisting of: peptides, proteins, enzymes, antibodies, lectin, nucleic acids, and bacterial tissue.

6. The bacterial biosensor of claim 5 wherein the selector molecules are hydrophilic.

7. The bacterial biosensor of claim 5 wherein the selector molecules are hydrophobic.

8. The bacterial biosensor of claim 5 wherein the selector molecules are B. Subtilis specific peptides.

9. The bacterial biosensor of claim 5 wherein the selector molecules are B. anthracis specific peptides.

10. The bacterial biosensor of claim 5 wherein the selector molecules are B. anthracis Sterne specific peptides.

11. The bacterial biosensor of claim 1 wherein the polysiloxane layer comprises a trichlorosilane compound.

12. The bacterial biosensor of claim 11 wherein the trichlorosilane compound is one selected from a group consisting of: octenyltrichlorosilane, cyclohexlmethyltrichlorosilane, bromopropyltrichlorosilane, trichlorosilane, tert-butyltrichlorosilane, ethoxytrichlorosilane, methyltrichlorosilane, and pentyltrichlorosilane.

13. The bacterial biosensor of claim 1 wherein the selector molecules are synthetic molecules that exhibit selective binding.

14. The bacterial biosensor of claim 1 wherein the selector molecules are cationic.

15. The bacterial biosensor of claim 1-wherein the selector molecules are anionic.

16. The bacterial biosensor of claim 1 wherein the substrate is a solid-state electronic device selected from the group consisting of: diodes, field effect transistors, insulated gate field effect transistors, and metal oxide semiconductor field effect transistors.

17. The bacterial biosensor of claim 2 wherein the substrate is an electrode including Metal-Oxide (MO) glass.

18. A bacterial biosensor comprising: a) a substrate having a layer of polymer applied thereto; and b) bacterial specific selector molecules wherein said biosensor is constructed by exposing the substrate to a suitable monomer in the presence of the bacterial specific selector molecules such that when the monomer is polymerized, the selector molecules are substantially immobilized thereto.

19. The bacterial biosensor of claim 18 wherein the bacterial specific selector molecules are peptides.

20. The bacterial biosensor of claim 19 wherein the substrate is an electrode including Indium-Tin-Oxide (ITO) glass.

21. The bacterial biosensor of claim 20 wherein the ITO glass is treated with NaOH.

22. The bacterial biosensor of claim 19 wherein the bacterial specific selector molecule is one selected from the group consisting of: peptides, proteins, enzymes, antibodies, lectin, nucleic acids, and bacterial tissue.

23. The bacterial biosensor of claim 22 wherein the selector molecules are hydrophilic.

24. The bacterial biosensor of claim 22 wherein the selector molecules are hydrophobic.

25. The bacterial biosensor of claim 22 wherein the selector molecules are B. Subtilis specific peptides.

26. The bacterial biosensor of claim 22 wherein the selector molecules are B. anthracis specific peptides.

27. The bacterial biosensor of claim 22 wherein the selector molecules are B. anthracis Sterne specific peptides.

28. The bacterial biosensor of claim 19 wherein the substrate is a solid-state electronic device.

29. The bacterial biosensor of claim 19 wherein the selector molecules are synthetic molecules that exhibit selective binding to specific bacterium.

30. The bacterial biosensor of claim 19 wherein the selector molecules include polylysine.

31. The bacterial biosensor of claim 9 wherein the selector molecules are cationic.

32. The bacterial biosensor of claim 9 wherein the selector molecules are anionic.

Brief Patent Description - Full Patent Description - Patent Claims

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