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  Affinity based system for detecting particulates in a fluidUSPTO Application #: 20060194264Title: Affinity based system for detecting particulates in a fluid Abstract: This invention provides methods and apparatus for detecting and quantifying particulate matter suspended in a fluid. Specifically, the invention provides an integrated, affinity-binding based, analytical system comprising a platform for performing an affinity-binding based assay for specifically binding particulates including microbial cells, and a detection means for detecting the particulates specifically bound to a defined surface or chamber comprising the platform. Methods for using the analytical systems of the invention are also provided. (end of abstract)
Agent: Mcdonnell Boehnen Hulbert & Berghoff LLP - Chicago, IL, US Inventors: Norman F. Sheppard, Alec Mian, Gregory Kellogg, Stephen G. Kieffer-Higgins, Bruce L. Carvalho USPTO Applicaton #: 20060194264 - Class: 435007900 (USPTO) Related Patent Categories: Chemistry: Molecular Biology And Microbiology, Measuring Or Testing Process Involving Enzymes Or Micro-organisms; Composition Or Test Strip Therefore; Processes Of Forming Such Composition Or Test Strip, Involving Antigen-antibody Binding, Specific Binding Protein Assay Or Specific Ligand-receptor Binding Assay, Assay In Which An Enzyme Present Is A Label The Patent Description & Claims data below is from USPTO Patent Application 20060194264. Brief Patent Description - Full Patent Description - Patent Application Claims
[0001] This application is a divisional of U.S. Ser. No. 10/726,337, filed Dec. 3, 2003, now U.S. Pat. No. 6,953,550, issued Oct. 11, 2005, which is a divisional of U.S. Ser. No. 09/989,259, filed Nov. 20, 2001, now U.S. Pat. No. 6,656,430, issued Dec. 2, 2003, which is a divisional of U.S. Ser. No. 09/614,834, filed Jul. 12, 2000, now U.S. Pat. No. 6,319,468, issued Nov. 20, 2001, which is a divisional application of U.S. Ser. No. 08/995,056, filed Dec. 19, 1997, now U.S. Pat. No. 6,143,247, issued Nov. 7, 2000, which claims priority to U.S. Provisional Application Ser. No. 60/034,327, filed Dec. 20, 1996. BACKGROUND OF THE INVENTION [0002] 1. Field of the Invention [0003] This invention relates to methods and apparatus for detecting, characterizing and quantifying particulate matter suspended in a fluid. More specifically, the invention provides an integrated, affinity-binding based analytical system for detecting particulates, particularly cells, suspended in a fluid, especially a biological fluid. In particular, the invention provides a platform for performing an affinity-binding based assay for specifically binding particulates including cells, and a detection means for detecting the particulates specifically bound to a defined surface or chamber comprising the platform. In addition, the invention provides such analytical systems to facilitate cell accumulation in a specific cell accumulation area or chamber of the platform, allowing particulate counting and characterization using the platform, as well as high throughput screening of test compounds to determine the capacity of the compound to affect cell viability, metabolism or physiology. Devices for manipulating the platforms of the invention are provided comprising detection means operatively arranged relative to the platform, as well as devices that provide detecting means for manually-manipulated platforms. Methods for using the platforms of the invention are also provided. [0004] 2. Background of the Related Art [0005] Determining the type, concentration and properties of particulates in a fluid is important in a variety of contexts. Dust and dirt particles in water, oil or other industrial fluids can negatively impact on the performance and useful lifetime of complex machinery. Pyrogens, including bacterial cells, in pharmaceutical products, or manufacturing facilities making such products, can compromise the safety and reliability of available drugs. Similarly, cells, particularly bacterial cells, that are themselves disease-causing (such as Salmonella spp.) or that make toxins (such as botulism toxin) are hazardous, and advantageously are screened in manufacturing and other settings where foodstuffs or other consumables are produced. Finally, mammalian cells, including sperm cells and hematopoietic cells, are usefully analyzed in the corresponding biological fluids for diagnostic and treatment monitoring purposes. [0006] Certain methods and apparatus for detecting biological molecules and cells are known in the prior art. [0007] U.S. Pat. No. 3,615,222 issued Oct. 26, 1971 to Mead discloses a specific binding method for detecting a component of a biological fluid. [0008] U.S. Pat. No. 3,743,482 issued Jul. 3, 1973 to Eisentraut discloses a method for determining thyroid function U.S. Pat. No. 3,907,502 issued Sep. 23, 1975 to Brink discloses a method for identifying Bence Jones proteins. [0009] U.S. Pat. No. 4,546,460 issued Oct. 8, 1985 to Ando discloses a videodisc autofocus device. [0010] U.S. Pat. No. 5,009,997 issued Apr. 23, 1991 to Shah et al. discloses two-site immunometric sandwich assay. [0011] U.S. Pat. No. 5,091,318 issued Feb. 25, 1992 to Anawis et al. discloses binding of allergens to a solid phase. [0012] International Application, Publication No. WO92/07243, published on Apr. 30, 1992 in the name of Cellpro, disclose the use of a biological particle separator. [0013] U.S. Pat. No. 5,137,031 issued Aug. 11, 1992 to Guirguis discloses a urine testing apparatus and cell collection. [0014] U.S. Pat. No. 5,278,048 issued Jan. 11, 1994 to Parce et al. discloses an apparatus for detecting the effect of a test compound on a living cell. [0015] U.S. Pat. No. 5,296,375, issued Mar. 22, 1994 to Kricka et al. disclose microplatforms for detecting the presence of an analyte in a fluid. [0016] U.S. Pat. No. 5,304,487, issued Apr. 19, 1994 to Kricka et al. disclose microplatforms for detecting the presence of an analyte in a fluid. [0017] International Application, Publication No. WO94/16543, published on Jul. 21, 1994 in the name of Schutze et al., disclose the use of a laser optical trap for manipulating living cells. [0018] U.S. Pat. No. 5,338,689 issued Aug. 16, 1994 to Yves et al. discloses a method for detecting antigens and antibodies. [0019] U.S. Pat. No. 5,403,720 issued Apr. 4, 1995 to Sato et al. discloses a method for detecting microorganisms. [0020] U.S. Pat. No. 5,427,946, issued Jun. 27, 1995 to Kricka et al. disclose microplatforms for detecting the presence of an analyte in a fluid. [0021] U.S. Pat. No. 5,451,504 issued Sep. 19, 1995 to Fitzpatrick et al. discloses a membrane strip for detecting the presence of an analyte in a sample. [0022] European Application, Publication No. EP634654, published on Oct. 4, 1995 in the name of Ventura disclose an apparatus for measuring purified water quality. [0023] U.S. Pat. No. 5,460,940 issued Oct. 24, 1995 to Yves et al. discloses a method for detecting antigens and antibodies. Continue reading... Full patent description for Affinity based system for detecting particulates in a fluid Brief Patent Description - Full Patent Description - Patent Application Claims Click on the above for other options relating to this Affinity based system for detecting particulates in a fluid patent application. ###  How KEYWORD MONITOR works... a FREE service from FreshPatents1. Sign up (takes 30 seconds). 2. Fill in the keywords to be monitored. 3. Each week you receive an email with patent applications related to your keywords. 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