FreshPatents.com Logo
stats FreshPatents Stats
1 views for this patent on FreshPatents.com
2013: 1 views
Updated: August 12 2014
newTOP 200 Companies filing patents this week


    Free Services  

  • MONITOR KEYWORDS
  • Enter keywords & we'll notify you when a new patent matches your request (weekly update).

  • ORGANIZER
  • Save & organize patents so you can view them later.

  • RSS rss
  • Create custom RSS feeds. Track keywords without receiving email.

  • ARCHIVE
  • View the last few months of your Keyword emails.

  • COMPANY DIRECTORY
  • Patents sorted by company.

Follow us on Twitter
twitter icon@FreshPatents

Carbohydrate-lipid constructs and their use in preventing or treating viral infection

last patentdownload pdfdownload imgimage previewnext patent


20120277172 patent thumbnailZoom

Carbohydrate-lipid constructs and their use in preventing or treating viral infection


The invention relates to selected carbohydrate-lipid constructs and their use as mimics of ligands for receptors expressed by a virus. In particular, the invention relates to the use of selected carbohydrate-lipid constructs in methods of inhibiting virus infection and/or promoting clearance of virus from infected subjects. Carbohydrate-lipid constructs selected for use in these methods where the virus is Human Immunodeficiency Virus (HIV) are provided.
Related Terms: Human Immunodeficiency Virus

Browse recent Kode Biotech Limited patents - Auckdland, NZ
Inventor: Stephen Michael Henry
USPTO Applicaton #: #20120277172 - Class: 514 25 (USPTO) - 11/01/12 - Class 514 
Drug, Bio-affecting And Body Treating Compositions > Designated Organic Active Ingredient Containing (doai) >O-glycoside

view organizer monitor keywords


The Patent Description & Claims data below is from USPTO Patent Application 20120277172, Carbohydrate-lipid constructs and their use in preventing or treating viral infection.

last patentpdficondownload pdfimage previewnext patent

TECHNICAL FIELD

The invention relates to selected carbohydrate-lipid constructs and their use as mimics of ligands for receptors expressed by a virus.

In particular, the invention relates to the use of selected carbohydrate-lipid constructs in methods of inhibiting viral infection and/or promoting clearance of virus from infected subjects.

Carbohydrate-lipid constructs selected for use in these methods where the virus is Human Immunodeficiency Virus (HIV) are provided.

BACKGROUND ART

Infection with HIV and Acquired Immune Deficiency Syndrome (AIDS) continues to increase worldwide, despite intense research to control its spread. Furthermore, the emergence of new viral infections presents additional challenges to public health.

Therapies to treat infection that target viruses may be limited in efficacy due to resistance and genetic variance of the virus.

HIV infection is mediated by the viral fusion glycoprotein gp120-gp41 binding the cell surface expressed receptor CD4. This binding is the basis of the viral targeting of T lymphocytes and monocyte macrophages. The receptor gp120 shows an affinity in vitro for several glycosphingolipids (GSLs) (Bhat et al (1993); Fantini et al (1998); Mylvaganam and Lingwood (1999a)).

A need exists for glycolipid mimics that are dispersible in biocompatible media and can be used to modify the interaction between naturally occurring membrane incorporated glycoconjugates, such as GSLs, and the receptors expressed by a virus. Such water soluble glycolipid mimics have been recognized as having potential for use in the preventative treatment of individuals at risk of infection from viruses such as HIV.

Lund et al (2006) have investigated the effect of the water soluble glycolipid mimic adamantylGb3 on HIV infection of cells in culture. In previous studies adamantylGb3 had been demonstrated to be a superior ligand for the receptor gp120 (Mahfoud et al (2002)).

A dose dependent inhibition of infection of Jurkat T cells by HIV-1 pre-incubated with adamantylGb3 has been demonstrated in vitro (Lund et al 2006). The in vivo inhibition of infection by HIV-1 was not reported, but the water soluble glycolipid mimic was indicated to have no effect on Jurkat T cell viability. Transient changes in CD4 surface expression were observed. Lund et al (2006) attributed the dose dependent inhibition of infection to an inhibition of attachment of the pre-treated HIV-1 to the Jurkat T cells. The adamantylGb3 treated virus remained non-host cell attached and virions could not be found within the Jurkat cells.

Further studies on HIV-1 infection of primary lymphoid cells in vitro provided results consistent with those observed for Jurkat T cells as host cells. Infection by both wild type and drug resistant HIV-1 infection was inhibited by the pre-treatment of the water soluble glycolipid mimic adamantylGb3. However, pre-incubation of cells with adamantylGb3 was ineffective.

Lund et al (2006) noted the effective concentration range required to inhibit HIV-1 infection would be difficult to maintain clinically, but suggested the formulation of adamantylGb3 within a cream might provide a topical ointment for the prevention of mucosal HIV infection.

It is an object of the invention to provide receptor binding carbohydrate-lipid constructs that are effective to inhibit viral infection of the cells of a subject.

It is a further object of the invention to provide receptor binding carbohydrate-lipid constructs that are effective to promote clearance of virus from an infected subject.

These objects are to be read disjunctively with the object of to at least provide a useful choice.

[Followed by Page 4] DISCLOSURE OF INVENTION

In a first aspect the invention provides a method of inhibiting infection of the cells of a subject by a virus by administering to the subject an amount of carbohydrate-lipid construct of the formula F-S1-S2-L where: F is selected from the group consisting of glycotopes of ligands for one or more receptors expressed by the virus; S1-S2 is a spacer linking F to L; and L is a lipid selected from the group consisting of diacyl- and dialkyl-glycerolipids, including glycerophospholipids.

Preferably, the amount is effective to inhibit binding of the receptor expressed by the virus to a cell surface expressed ligand.

Preferably, the receptor is expressed by the human immunodeficiency virus (HIV).

S1-S2 is selected to provide a carbohydrate-lipid construct that is dispersible in water.

Preferably, S1 is a C2-5-aminoalkyl selected from the group consisting of: 2-aminoethyl; 3-aminopropyl; 4-aminobutyl; and 5-aminopentyl.

Preferably, S2 is selected from the group consisting of: —CO(CH2)3CO—; —CO(CH2)4CO— (adipate); and —CO(CH2)5CO—.

Preferably, L is selected from the group consisting of: diacylglycerolipids, phosphatidate, phosphatidyl choline, phosphatidyl ethanolamine, phosphatidyl serine, phosphatidyl inositol, phosphatidyl glycerol, and diphosphatidyl glycerol derived from one or more of trans-3-hexadecenoic acid, cis-5-hexadecenoic acid, cis-7-hexadecenoic acid, cis-9-hexadecenoic acid, cis-6-octadecenoic acid, cis-9-octadecenoic acid, trans-9-octadecenoic acid, trans-11-octadecenoic acid, cis-11-octadecenoic acid, cis-11-eicosenoic acid or cis-13-docsenoic acid. More preferably, the lipid is derived from one or more cis-destaurated fatty acids. Most preferably, L is selected from the group consisting of: 1,2-O-dioleoyl-sn-glycero-3-phosphatidylethanolamine (DOPE) and 1,2-O-distearyl-sn-glycero-3-phosphatidylethanolamine (DSPE); and rac-1,2-dioleoylglycerol (DOG).

Preferably, L is a glycerophospholipid and the construct includes the substructure:

where X is H or C, * is other than H and n is an integer 2 to 5.

More preferably, L is a glycerophospholipid and the construct includes the substructure:

where: X is H; R1 is a Cp-alkyl glycoside, R2 and R3 are independently selected from the group consisting of: trans-3-hexadecenal, cis-5-hexadecenal, cis-7-hexadecenal, cis-9-hexadecenal, cis-6-octadecenal, cis-9-octadecenal, trans-9-octadecenal, trans-11-octadecenal, cis-11-octadecenal, cis-11-eicosenal and cis-13-docsenal; n is 2 to 5; and p is 2 or

Download full PDF for full patent description/claims.

Advertise on FreshPatents.com - Rates & Info


You can also Monitor Keywords and Search for tracking patents relating to this Carbohydrate-lipid constructs and their use in preventing or treating viral infection patent application.
###
monitor keywords



Keyword Monitor How KEYWORD MONITOR works... a FREE service from FreshPatents
1. Sign up (takes 30 seconds). 2. Fill in the keywords to be monitored.
3. Each week you receive an email with patent applications related to your keywords.  
Start now! - Receive info on patent apps like Carbohydrate-lipid constructs and their use in preventing or treating viral infection or other areas of interest.
###


Previous Patent Application:
Warmi®, natural alternative for the treatment of menopause
Next Patent Application:
Stabilized anthocyanin compositions
Industry Class:
Drug, bio-affecting and body treating compositions
Thank you for viewing the Carbohydrate-lipid constructs and their use in preventing or treating viral infection patent info.
- - - Apple patents, Boeing patents, Google patents, IBM patents, Jabil patents, Coca Cola patents, Motorola patents

Results in 0.68907 seconds


Other interesting Freshpatents.com categories:
Computers:  Graphics I/O Processors Dyn. Storage Static Storage Printers

###

Data source: patent applications published in the public domain by the United States Patent and Trademark Office (USPTO). Information published here is for research/educational purposes only. FreshPatents is not affiliated with the USPTO, assignee companies, inventors, law firms or other assignees. Patent applications, documents and images may contain trademarks of the respective companies/authors. FreshPatents is not responsible for the accuracy, validity or otherwise contents of these public document patent application filings. When possible a complete PDF is provided, however, in some cases the presented document/images is an abstract or sampling of the full patent application for display purposes. FreshPatents.com Terms/Support
-g2-0.2547
     SHARE
  
           

FreshNews promo


stats Patent Info
Application #
US 20120277172 A1
Publish Date
11/01/2012
Document #
13459399
File Date
04/30/2012
USPTO Class
514 25
Other USPTO Classes
International Class
/
Drawings
9


Human Immunodeficiency Virus


Follow us on Twitter
twitter icon@FreshPatents