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Ip-10 based immunological monitoring

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Title: Ip-10 based immunological monitoring.
Abstract: The present invention relates to an immunological method and, more particularly, a method for measuring cell-mediated immune reactivity (CMI) in mammals based on the production of IP-10. The invention further discloses an assay and a kit for measuring CMI to an antigen using whole blood or other suitable biological samples. The methods of the present invention are useful in therapeutic and diagnostic protocols for human, livestock and veterinary and wild life applications, thus the invention further relates to a method for diagnosing an infection in a mammal. ...


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Inventors: Morten Ruhwald, Pernille Ravn, Jesper Eugen-Olsen
USPTO Applicaton #: #20120015386 - Class: 435 792 (USPTO) - 01/19/12 - Class 435 
Chemistry: Molecular Biology And Microbiology > Measuring Or Testing Process Involving Enzymes Or Micro-organisms; Composition Or Test Strip Therefore; Processes Of Forming Such Composition Or Test Strip >Involving Antigen-antibody Binding, Specific Binding Protein Assay Or Specific Ligand-receptor Binding Assay >Assay In Which An Enzyme Present Is A Label >Heterogeneous Or Solid Phase Assay System (e.g., Elisa, Etc.)

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The Patent Description & Claims data below is from USPTO Patent Application 20120015386, Ip-10 based immunological monitoring.

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CROSS-REFERENCE TO RELATED APPLICATIONS

This application is a divisional of and claims the benefit of priority to U.S. patent application Ser. No. 12/438,515, filed Oct. 14, 2009, which is a National Phase application of and claims the benefit of priority to International Application No. PCT/DK2007/000399, filed on Sep. 5, 2007, designating the United States of America and published in the English language, which is an International Application of and claims the benefit of priority to Danish Patent Application No. PA200601145, filed Sep. 5, 2006, and Danish Patent Application No. PA200700262, filed Feb. 20, 2007. The disclosures of the above-referenced applications are hereby expressly incorporated by reference in their entireties.

FIELD OF INVENTION

The present invention relates generally to an immunological assay and, more particularly, an assay for measuring cell-mediated immune reactivity (CMI). Even more particularly, the present invention provides an assay and a kit for measuring a cell-mediated response to an antigen using whole blood or other suitable biological samples. The assay is useful in therapeutic and diagnostic protocols for human, livestock and veterinary and wild life applications.

Measurement of cell-mediated immune responses is important for immune diagnosis of many infectious and autoimmune diseases, as a marker for immunocompetence, and for detection of T-cell responses to endogenous and exogenous antigens (i.e. infections and vaccines).

The present invention provides a method for measuring CMI in a mammal by incubating a sample from the mammal which comprises T-cells or other cells of the immune system with an antigen. Production of IP-10 is then detected. The presence or level of immune effecter is then indicative of the level of cell mediated responsiveness of the subject.

BACKGROUND OF THE INVENTION

Tuberculosis

The discovery of mycobacterium tuberculosis (MTB)-specific immunodominant antigens has led to a significant new avenue for the diagnosis of tuberculosis (TB). Early work had shown the potential to replace the Tuberculin Skin Test (TST) by a test that assayed the in vitro production of interferon gamma (IFN-γ) by T cells in response to defined MTB antigens. Around the same time, a major advance was the discovery of the highly immunogenic antigens, early secreted antigenic target 6 (ESAT-6) and culture filtrate protein 10 (CFP-10) and TB7.7 that improved specificity significantly. These antigens are encoded within the region of difference 1 (RD1) of the pathogen and are consequently absent from all Bacille Calmette Guerin (BCG) vaccine strains and most non-tuberculous mycobacteria (exceptions include Mycobacterium kansasii, Mycobacterium marinum Mycobacterium szulgai). IFN-γ responses to overlapping peptides of the RD1 encoded antigens ESAT-6, CFP-10, TB7.7 form the basis for the detection of MTB infection in two licensed and commercially available tests.

QuantiFERON-TB Gold (Cellestis Limited, Carnegie, Victoria, Australia), a whole blood enzyme-linked immunoassay (ELISA) has European CE mark approval and recently received American Food and Drug Administration (FDA) approval for the detection of both latent TB infection and disease.

T-SPOT.TB (Oxford Immunotec, Oxford, UK), an enzyme-linked immunospot assay (ELISPOT) that uses peripheral blood mononuclear cells has European CE mark approval and was approved for use in Canada in 2005. T-SPOT.TB only uses ESAT-6 and CFP10.

However, the limitations of the currently available tests are: 1) The sensitivity may be impaired in immunosuppressed individuals (such as HIV positive or patients receiving immunosupressing medication), 2) In some situations a relatively large volume of blood is necessary (3 ml per QuantiFERON test and 8 ml for the T-SPOT.TB), which may limit its use in infants and severely ill and anaemic children, 3) The tests do not discriminate between active, latent and recent infection 4) The tests have not been demonstrated to be able to predict who will progress from recent or latent TB to active TB.

Most of the test limitations are due to measurement of the effect parameter IFN-γ at very low levels, close to the limit of even the most sensitive method (in the QuantiFERON test down to 0.35 IU/ml (17.5 pg/ml) and in the T-SPOT.TB 5 spots/field). Decreasing cut-off to enhance sensitivity will eventually result in impaired specificity of the tests. A recent publication based on the QuantiFERON test has shown that that repeated testing of people with test results in the lower range of IFN-γ varies around the cut-off level which underlines the potential risk of false positive and false negative results of the QuantiFERON (QFT) test (Pai, M. et al).

To overcome the evident fragility of the tests, sensitivity could be improved by using additional M. tuberculosis specific antigens and this has been done in the third generation of the QuantiFERON tests (QFT), the QuantiFERON In tube test (QFT-IT) which now comprises an additional antigen named TB7.7(p4) and potentially sensitivity is improved, but it still depends on measurements at very low IFN-γ levels.

This approach has been tried by others, i.e. recently is was shown that the Monokine Induced by IFN-γ (MIG/CXCL9) was specifically expressed in vitro after stimulation with M. tuberculosis specific antigens (ESAT-6/CFP10) and PPD. The sensitivity of CXCL9, however was very low and lower than that of IFN-γ. Another smaller study based on intracellular cytokine cytometry in CD4+ T cells following ESAT-6 stimulation, tested if expression of IFN-γ, IL-2, IL-4, IL-10 or the activation marker CD40L could distinguish TB from non-TB disease. None of these markers were found to be comparable or superior to IFN-γ (Abramo C, et al) (Hughes, A. et al).

Yet no sensitive and specific markers to replace IFN-γ for diagnosis of TB infection have been identified in the presently published literature. Various have disclosed IP-10 in connection to infections, but not as a marker in the diagnosis of infection with a prior antigen stimulation.

Chlamydia

The diagnosis of genital chlamydial infections evolved rapidly from the 1990s. Nucleic acid amplification tests (NAAT), such as polymerase chain reaction (PCR), transcription mediated amplification (TMA), and the DNA strand displacement assay (SDA) now are the mainstays. The most commonly used and widely studied chlamydia NAATs in the US and many other industrialized countries are Aptima (Gen-Probe), Probe-Tec (Becton-Dickinson), and Amplicor (Roche). The Aptima Combo II assay tests simltaneously for C. trachomatis and Neisseria gonorrhoeae, the cause of gonorrhea. NAAT for chlamydia may be performed on swab specimens collected from the cervix (women) or urethra (men)

At present, the NAATs have regulatory approval only for testing urogenital specimens. The NAATs have largely replaced culture, the historic gold standard for chlamydia diagnosis, and the non-amplified probe tests, such as Pace II (Gen-Probe). The latter test is relatively insensitive, successfully detecting only 60-80% of infections in asymptomatic women, and occasionally giving falsely positive results. Culture remains useful in selected circumstances and is currently the only assay approved for testing non-genital specimens.

Chlamydia diagnosis is thus based on complicated and resource demanding technology such as PCR, not readily available in the developing world. A fast and easy technology would improve diagnostic measures for this important disease,

CA 2,478,138 discloses that elevated blood levels of the chemokine CXCL10 polypeptide are associated with respiratory illnesses (e.g. SARS, influenza and community-acquired pneumonia) and are useful in diagnosis of patients. Methods are provided for diagnosis and treatment of patients suffering from respiratory illnesses.

WO 05/091969 discloses markers for TSE (Transmissible Spongiform Encephalopaties) that are present prior to formation of detectable pathological prion protein are useful to detect this infection prior to clinical signs. IP-10 is just one of several markers disclose and this application does not disclose any antigen stimulation.

US2004-038201 discloses distinct gene expression programs activated in response to different pathogens in macrophages. IP-10 is again just one in many markers mentioned and this application does not disclose any antigen stimulation.

Annalisa Azzurri et al. discloses IFN-γ-inducible protein 10 and pentraxin 3 plasma levels are tools for monitoring inflammation and disease activity in Mycobacterium Tuberculosis infection. The article shows that IP-10 plasma level is spontaneously increased in patients with TB, again this reference does not disclose any antigen stimulation.

WO 03/063759 discloses a method of identifying a heat shock protein (Hsp) derived peptide useful for diagnosis or therapy. The effect of the compounds of this invention was tested on pheripheral blood monocytes by measuring the IP-10 level in response to subsequent stimulation with LPS. Direct stimulation with the test compounds without subsequent stimulation with LPS, did not give rise to an increase in the IP-10 level.

WO 07/039,400 discloses a method and kit for the diagnosis of Immune Restoration Syndrome which is associated with tuberculosis (TB-IRS) in patients infected with tuberculosis as well as HIV/TB co-infected patients. In order to diagnose TB-IRS the inventors detected the level of Th1 response against PPD and/or a 16 KDA protein in comparison to the Th1 response against ESAT-6, CFP-10, 85B (negative control) and used a rise in the Th1 response as an indicative of TB-IRS.

To overcome the problems of impaired sensitivity and low levels of detectable IFN-γ by the currently available tests using antigen stimulation, the present inventors suggest the use of an alternative biomarker than IFN-γ.

SUMMARY

OF THE INVENTION

The present invention proposes a novel diagnostic principle. A test system that can detect infection with e.g. tuberculosis eishmania or chlamydia, based on measuring the chemokine IP-10 following stimulation of immune cells with antigenic proteins/peptides.

The described test system IP-10 is more sensitive than tests based on IFN-γ as effect parameter, it improves testing and diagnosing. The test can be performed using lower amounts of blood because the samples can be diluted at time of incubation or before analysis. The test can be performed at shorter incubation times. Furthermore, the test system may allow discriminating between various stages of infection such as e.g. active, recent and latent TB infection.

In short the present invention can be described as an immunological method comprising the steps of incubating a sample obtained from a mammal with at least one antigen, determining the IP-10 level in said sample and comparing said determined IP-10 level with a reference-level, thereby determining whether mammal has previously encountered the antigen generating immunological reactivity to the antigen, or previously encountered other antigens generating immunological cross reactivity to the antigen.

DETAILED DESCRIPTION

The present invention provides an assay of the potential or capacity of a subject to mount a CMI response. The assay is based on measuring immune effecter molecule production by cells of the immune system in response to antigenic stimulation. The immune effecters may be detected using ligands such as antibodies specific for the effecters or by measuring the level of expression of genes encoding the effecters.

The present invention provides, therefore, means to determine the responsiveness of CMI in a subject and, in turn, provides means for the diagnosis of infectious diseases, pathological conditions, level of immunocompetence and a marker of T-cell responsiveness to endogenous or exogenous antigens

One aspect of the invention relates to an assay of the potential or capacity of a subject to mount an IP-10 response. The assay is based on measuring IP-10 production by cells of the immune system in response to antigenic stimulation. The IP-10 production may be detected using ligands such as antibodies specific for IP-10 or by measuring the level of expression of genes encoding IP-10. The present inventors have demonstrated the test principle using two different types of infection: Tuberculosis and Chlamydia. In the case of tuberculosis a test based on M. Tuberculosis specific stimulation and subsequent determination of IP-10, can identify persons infected with M. tuberculosis. In the case of Chlamydia a test based on stimulation with C.Trachomatis extract can identify persons infected with Chlamydia.

The described test system measures higher levels of the biomarker IP-10, than the levels of IFN-γ, the marker the currently available assays are based on. The test system based on IP-10 is as specific as and more sensitive than tests based on e.g. IFN-γ as effect parameter, it improves testing and diagnosing of immunocompromised individuals (Example 10), the test can be performed using lower amounts of blood because the samples can be diluted at time of incubation or before analysis (Examples 9 and 13). It also improves the speed of diagnosis, as IP-10 is produced in significant amounts after few hours of incubation as shown in example 11. In the case of tuberculosis, the test system may allow discriminating between active, recent and latent TB infection and, in addition the test system is potentially capable of identifying persons at risk of progressing to active TB. The test system is based on IP-10 detection using an immunoassay (i.e. ELISA or Luminex) and the test system can potentially be developed into a field friendly immunochromatographic test applicable in low resource settings, where the test result is presented by a colour reaction detectable to the naked eye.

The assay described in the present invention solves a series of problems. The currently available assays measure the effect parameter IFN-γ at very low levels, close to the limit of even the most sensitive detection method (in the case of tuberculosis tests, the QuantiFERON test has a cut-off level for positive test at 0.35 international units/ml (17.5 pg/ml) and in the T-SPOT.TB test 5 spot forming units/field). Decreasing cut-off to enhance sensitivity will eventually result in impaired specificity of the tests. Publications based on the Quantiferon test have shown that that repeated testing of people with test results in the lower range of IFN-γ varies around the cut-off level which underlines the potential risk of false positive and false negative results of the Quantiferon (QFT) test. In addition the current test may give false negative results in immunosuppressed individuals who are unable to mount an IFN-γ response above the cut-off level. As the amount of IP-10 release is much higher after antigen stimulation when compared to IFN-γ: the sensitivity is higher, fewer tests are deemed false negative, test results are more reproducible, and, less indeterminate test results are expected in immunosuppressed individuals.

Furthermore, because antigen induced IP-10 is secreted in such high concentrations it is possible to dilute the sample before or after the incubation step. This means that the amount of sample material (e.g. whole blood) needed to perform the test can be reduced e.g. in the case of whole blood down to or even at or below 0.25 ml, such as 0.20 ml, e.g. 0.15 ml, such as 0.1 ml, e.g 0.05 ml. In a preferred embodiment the test can be performed down to or even below 0.1 ml. Thus a “mini assay” suitable for patients with low blood volume (e.g. children/infants or anaemics) can be developed. Furthermore, using e.g. blood from e.g. a finger-prick, the mini assay can be made even more user friendly as vein puncture is avoided.

Furthermore, in the case of tuberculosis none of the currently available tests can discriminate between Active and Latent infection. Surprisingly the inventors found the concentration of IP-10 in the un-stimulated, but incubated sample material i.e. the nil sample (e.g. whole blood), is higher among patients with active disease compared to healthy individuals with latent disease. The present inventors propose that the IP-10 concentration in sample material incubated with an inactive solution (Nil) in combination with an antigen-specific test can be used as marker for active infection (e.g. tuberculosis) versus latent infection (e.g. tuberculosis).

The Assay

Thus, one aspect of the present invention relates to an immunological method comprising the steps of a) incubating a sample obtained from a mammal with at least one test-antigen

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stats Patent Info
Application #
US 20120015386 A1
Publish Date
01/19/2012
Document #
13245521
File Date
09/26/2011
USPTO Class
435/792
Other USPTO Classes
435 34
International Class
/
Drawings
5



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