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Method of modifying target region in host dna and selectable marker cassette

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Title: Method of modifying target region in host dna and selectable marker cassette.
Abstract: A method of modifying a target region in a host DNA using a donor DNA: wherein the donor DNA having regions homologous to a 5′-side region outside of the target region in the host DNA, a 3′-side region outside of the target region in the host DNA and a first homologous recombination region inside of the target region in the host DNA, respectively, in this order, and further having a first selectable marker gene, an expression-inducing promoter and a second selectable marker gene expressed under the control of the expression-inducing promoter between the region homologous to the 3′-side region and the region homologous to the first homologous recombination region; which method has the steps of: a first step of performing homologous recombination between the donor DNA and the host DNA at the regions of the 5′-side region and the first homologous recombination region, to conduct selection of a host integrated with the donor DNA based on expression of the first selectable marker gene; and a second step of performing homologous recombination, within the host DNA integrated with the donor DNA by the first step, between two regions of the 3′-side region derived from the host DNA and the 3′-side region derived from the donor DNA, to conduct selection of a host whose target region is modified based on expression of the second selectable marker gene under an expression-inducing condition for the expression-inducing promoter; and a selectable marker cassette for use in the method. ...


Browse recent Kao Corporation patents - Tokyo, JP
Inventors: Katsutoshi ARA, Takuya MORIMOTO, Naotake OGASAWARA
USPTO Applicaton #: #20110275158 - Class: 435490000 (USPTO) - 11/10/11 - Class 435 


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Related Patent Categories: Chemistry: Molecular Biology And Microbiology, Process Of Mutation, Cell Fusion, Or Genetic Modification, Introduction Of A Polynucleotide Molecule Into Or Rearrangement Of Nucleic Acid Within A Microorganism (e.g., Bacteria, Protozoa, Bacteriophage, Etc.), The Polynucleotide Is An Unbranched Linear Fragment
The Patent Description & Claims data below is from USPTO Patent Application 20110275158, Method of modifying target region in host dna and selectable marker cassette.

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stats Patent Info
Application #
US 20110275158 A1
Publish Date
11/10/2011
Document #
File Date
08/30/2014
USPTO Class
Other USPTO Classes
International Class
/
Drawings
0


Homologous
Marker
Marker Gene
Promoter
Recombination
Selection


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