FreshPatents.com Logo
stats FreshPatents Stats
27 views for this patent on FreshPatents.com
2014: 1 views
2013: 4 views
2012: 6 views
2011: 10 views
2010: 6 views
Updated: June 10 2014
newTOP 200 Companies filing patents this week


    Free Services  

  • MONITOR KEYWORDS
  • Enter keywords & we'll notify you when a new patent matches your request (weekly update).

  • ORGANIZER
  • Save & organize patents so you can view them later.

  • RSS rss
  • Create custom RSS feeds. Track keywords without receiving email.

  • ARCHIVE
  • View the last few months of your Keyword emails.

  • COMPANY DIRECTORY
  • Patents sorted by company.

Follow us on Twitter
twitter icon@FreshPatents

Bioreactor

last patentdownload pdfimage previewnext patent


Title: Bioreactor.
Abstract: The present invention relates to a bioreactor comprising a first fluid distribution chamber and a first fluid collection chamber, the reactor adapted to receive at least one conduit in fluid communication between the first fluid distribution chamber and the first fluid collection chamber; wherein the reactor includes a second fluid distribution means including a plurality of distributors arranged to distribute the second fluid between the first fluid distribution chamber and the first fluid collection chamber. The invention extends to a removable insert for a bioreactor comprising a first fluid distribution plate; a first fluid collection plate; and a second fluid distribution means including a plurality of distributors arranged to distribute the second fluid between the first fluid distribution plate and the first fluid collection plate. ...


USPTO Applicaton #: #20090311777 - Class: 4352972 (USPTO) - 12/17/09 - Class 435 
Chemistry: Molecular Biology And Microbiology > Apparatus >Bioreactor >Including Semipermeable Membrane Or Filter >Including Perfusion Means

view organizer monitor keywords


The Patent Description & Claims data below is from USPTO Patent Application 20090311777, Bioreactor.

last patentpdficondownload pdfimage previewnext patent

BACKGROUND OF THE INVENTION

This invention relates to a bioreactor. In particular this invention relates to a bioreactor including including membrane conduits and being adapted to received a first and second fluid.

In the biotechnology and biopharmaceutical industry, the most relevant bio-origin compounds are produced using bioprocesses involving specific cell culture systems operated and controlled within a cell culture bioreactor or module.

In general, these cell-culturing systems are characterized by several process limitations as well as fundamental-physical constraints delimiting the maximal production capacity of these established generic technologies. These limitations are expressed fundamentally as limits in mass transfer capabilities of these technologies. Examples of such known technologies include pneumatic reactors, solid state reactors and membrane conduit bioreactors.

It is obvious that the limitations mentioned above have a negative impact on the cost-effectiveness of these reactors and on the efficacy with which they function.

Furthermore, usually a specific process demands a specific type of reactor and therefore it can be extremely costly to have to purchase new process-specific reactors as they are required.

A need exist for an improved bioreactor.

A further need exists for improved reactors which are either adapted to the purpose required or which can be adapted to such purpose in a commercially viable manner.

SUMMARY

OF THE INVENTION

According to a first aspect to the present invention there is provided a bioreactor comprising: a first fluid distribution chamber; and a first fluid collection chamber, the reactor adapted to receive at least one conduit in fluid communication between the first fluid distribution chamber and the first fluid collection chamber; wherein the reactor includes a second fluid distribution means including a plurality of distributors arranged to distribute the second fluid between the first fluid distribution chamber and the first fluid collection chamber.

The bioreactor may include a plurality of conduits in fluid communication between the first fluid distribution chamber and the first fluid collection chamber. The conduits are preferably membrane conduits. In a preferred embodiment of the present invention the conduits are axially elongate having first and second ends (resembling drinking straws). Such conduits are described in U.S. Pat. No. 5,945,002 (Leukes et al.) and US Publication No. 2004/0191855 A1 (Leukes et al.), the contents of both of which are included herein by reference.

The first end of the conduits are preferably adapted to engage in the first fluid distribution chamber and the second end of the conduit is adapted to engage with the first fluid collection chamber.

The first fluid may be a liquid, for example a liquid nutrient. The second fluid may be a gas, for example, oxygen, nitrogen or a mixture thereof.

Preferably the distributors distribute the second fluid amongst the conduits. The distributors preferably distribute the second fluid in a direction transverse to a longitudinal axis of the conduits. The distributors preferably extend between the first fluid distribution chamber and the first fluid collection chamber and may be substantially parallel with the conduits. There are preferably at least two distributors, more preferably at least three distributors, more preferably at least four distributors, more preferably at least five distributors, more preferably at least six distributors, more preferably at least seven distributors and more preferably at least eight distributors.

The chambers preferably include perforated plates. The conduits may engage with or within the perforations. The conduits may engage by epoxy sealants and/or clamping plates.

The chambers are preferably spaced from each other with the conduits extending between them.

The bioreactor may include a skin defining a lumen between the chambers. The bioreactor may include a second outer skin. The outer skin may be adapted to receive temperature modifying fluids in a space between the two skins.

The second fluid distribution means preferably includes a chamber. The second fluid distribution means may include a manifold.

The bioreactor may include spacer means, for example, bars separating the first fluid distribution chamber from the first fluid collection chamber.

In one embodiment of this aspect of the present invention the perforated plates, conduits and second fluid distribution means comprise a removable insert. The insert may engage with a framework of the bioreactor.

According to a second aspect to the present invention there is provided a removable insert for a bioreactor comprising a first fluid distribution plate; a first fluid collection plate; and a second fluid distribution means including a plurality of distributors arranged to distribute the second fluid between the first fluid distribution plate and the first fluid collection plate.

According to a third aspect to the present invention there is provided a bioreactor comprising a frame adapted to receive a removable insert, the bioreactor comprising a first fluid inlet and distribution means, a second fluid inlet and distribution means, a first fluid collection means and outlet and a second fluid outlet.

Preferably the second fluid distribution means includes a plurality of distributors arranged to distribute the second fluid between the first fluid distribution plate and the first fluid collection plate.

Preferably the removable insert comprises a lumen and means for effecting fluid communication between the first fluid distribution means and first fluid collection means. The means for effecting fluid communication is preferably a conduit, most preferably a membrane conduit. In a preferred embodiment of the present invention the conduits are axially elongate having first and second ends (resembling drinking straws). The first end of the conduits are adapted to engage in the first fluid distribution means and the second end of the conduit is adapted to engage with the first fluid collection means.

The first fluid distribution means preferably comprises a distribution reservoir (chamber) defined by the frame, a distribution plate and a base. The distribution plate defines at least one perforation in which a first end of the membrane conduits is adapted to engage. The same is true for the first fluid collection means which preferably comprises a collection reservoir (chamber) defined by the frame, a collection plate and a cap. The collection plate defines perforations into which the second ends of the conduits engage. It will be appreciated that the ends of the membrane conduits may first be engaged, co-axially or otherwise, in pots or the like which in turn engage with the perforations in the distribution or collection plates. Epoxy sealants may also be used. In addition, sealing plates may also be used

The cap and the base are preferably removably attachable to the frame and/or insert and may be configured to accommodate the first and/or second fluid inlets and/or outlets.

The distribution plate and/or the collection plate are preferably included on the insert.

In a preferred embodiment, the first fluid inlet is in fluid communication with the distribution reservoir. Similarly, the first fluid outlet is in fluid communication with the collection reservoir. In use the first fluid enters the distribution reservoir via the first fluid inlet, passes through the perforations in the distribution plate, through the membrane conduits, through the perforations in the collection plate and into the collection reservoir where the first fluid exits the bioreactor via the first fluid outlet.

Preferably the first fluid is a liquid, for example a liquid (nutrient) medium suitable to sustain growth of micro-organisms and the second fluid is a gas, for example air. Examples of micro-organisms which may be sustained by the medium include bacteria and fungi including but not limited to Streptomyces coelicolor (aerobic process mode) and Lactococcus lactis (anaerobic process mode).

The membrane conduits may be comprised of a polymer material or a ceramic material. Preferably the conduits are comprised of a ceramic material, more preferably Al2O3. This allows for autoclave sterilization, and chemical (for example H2O2) cleaning without damage to the membrane conduits or housing. The membrane conduits are typically rigid (as opposed to flexible, in the case of polymer membrane conduits), which facilitates assembly of the insert and bioreactor, whereby touching of the membrane conduits can be minimised. The ceramic membrane conduit wall allows for good attachment of micro-organisms (as hereinbefore described), and the environment might stimulate differentiation in soil adapted organisms.

Preferably a plurality of the membrane conduits join the first fluid distribution and collection means. The membrane conduits may be pre-selected depending on the particular application of the bioreactor. Consistent spacing of the membrane conduits may be accurately achieved, and the spacing may be optimized for each application. Spacing of the membrane conduits is effected by suitable placing of perforations in the distribution and collection plates.

Preferably the second fluid distribution means is included within the removable insert. The second fluid distribution means preferably comprises a manifold in fluid communication the second fluid inlet and with fluid distributors located within the lumen of the insert. The fluid dispensing means may be at least one axially extending conduit positioned adjacent and preferably substantially parallel the membrane conduit(s). The fluid distributors may be adapted to effect transverse movement of the second fluid relative to a longitudinal axis of the membrane conduit(s). This may be achieved by a series of outlets located along the length of the fluid distributors. The outlets are preferably dimensioned such that substantially equal entry velocities of the second fluid into the insert lumen is effected across all outlets of the fluid dispensing means. Alternatively, the outlets may be so dimensioned such that substantially equal exit velocities of the second fluid from the distributors is effected across the outlets.

This may be achieved by making the outlets such that the resistance provided by the outlet is substantially greater than the resistance between each outlet.

The insert may include or be contained within a sleeve. The sleeve preferably allows visual inspection of the membrane conduits.



Download full PDF for full patent description/claims.

Advertise on FreshPatents.com - Rates & Info


You can also Monitor Keywords and Search for tracking patents relating to this Bioreactor patent application.
###
monitor keywords



Keyword Monitor How KEYWORD MONITOR works... a FREE service from FreshPatents
1. Sign up (takes 30 seconds). 2. Fill in the keywords to be monitored.
3. Each week you receive an email with patent applications related to your keywords.  
Start now! - Receive info on patent apps like Bioreactor or other areas of interest.
###


Previous Patent Application:
Stirred tank bioreactor
Next Patent Application:
Supply system for cell culture module
Industry Class:
Chemistry: molecular biology and microbiology
Thank you for viewing the Bioreactor patent info.
- - - Apple patents, Boeing patents, Google patents, IBM patents, Jabil patents, Coca Cola patents, Motorola patents

Results in 0.54496 seconds


Other interesting Freshpatents.com categories:
QUALCOMM , Monsanto , Yahoo , Corning ,

###

Data source: patent applications published in the public domain by the United States Patent and Trademark Office (USPTO). Information published here is for research/educational purposes only. FreshPatents is not affiliated with the USPTO, assignee companies, inventors, law firms or other assignees. Patent applications, documents and images may contain trademarks of the respective companies/authors. FreshPatents is not responsible for the accuracy, validity or otherwise contents of these public document patent application filings. When possible a complete PDF is provided, however, in some cases the presented document/images is an abstract or sampling of the full patent application for display purposes. FreshPatents.com Terms/Support
-g2-0.2355
     SHARE
  
           

FreshNews promo


stats Patent Info
Application #
US 20090311777 A1
Publish Date
12/17/2009
Document #
12296889
File Date
03/27/2007
USPTO Class
4352972
Other USPTO Classes
International Class
12M1/12
Drawings
10



Follow us on Twitter
twitter icon@FreshPatents