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Phytase variantsPhytase variants description/claimsThe Patent Description & Claims data below is from USPTO Patent Application 20090029004, Phytase variants. Brief Patent Description - Full Patent Description - Patent Application Claims
CROSS-REFERENCE TO RELATED APPLICATIONS
This application is a continuation of U.S. application Ser. No. 10/358,960 filed on Feb. 5, 2003, which claims, under 35 U.S.C. 119, priority from or the benefit of Danish application nos, PA 2002 00193 and PA 2002 01449 filed Feb. 8, 2002 and Sep. 30, 2002, respectively, and U.S. provisional application Nos. 60/356,392 and 60/416,348, filed Feb. 12, 2002 and Oct. 4, 2002, respectively, the contents of which are fully incorporated herein by reference. SEQUENCE LISTINGThis application present application contains information in the form of a sequence listing, which is appended to the application and also submitted on a data carrier accompanying this application. The contents of the data carrier are fully incorporated herein by reference. BACKGROUND OF THE INVENTION1. Field of the Invention The present invention relates to variants of a parent phytase, in particular variants of improved thermostability and/or specific activity. The invention also relates to DNA sequences encoding such variants, their production in a recombinant host cell, as well as methods of using the variants, in particular within the field of animal feed. A preferred parent phytase is the Peniophora phytase comprising SEQ ID NO, 2. 2. Description of Related Art EP 897010 entitled “Modified phytases” discloses, i.a., certain variants of an Aspergillus fumigatus phytase. EP 897985 entitled “Consensus phytases” discloses, i.a., a fungal consensus phytase which may be designed on the basis of, i.a., a multiple alignment of several ascomycete phytases. WO 99/48380 entitled “Thermostable phytases in feed preparation and plant expression” relates to certain aspects of using thermostable phytases. Examples of thermostable phytases are the various consensus phytases listed at p. 30 below the bold line, WO 00/43503 entitled “Improved phytases” relates i.a. to certain phytase variants of increased thermostability, which may be designed by a process similar to the one described in EP 897985. Examples of thermostable phytases are shown at p. 54-55 below the bold line. These phytases all have a percentage of identity to SEQ ID NO: 2 of below 75%. WO 98/28408 discloses a phytase derived from Peniophora lycii. WO 99/49022 discloses certain variants of Peniophora lycii phytase. It is an object of the present invention to provide novel and improved phytase variants, in particular of improved thermostability and/or increased specific activity. SUMMARY OF THE INVENTIONThe present invention relates to a variant of a parent phytase, comprising a substitution in at least one position of at least one region selected from the group of regions consisting of: 20; 26; 28-31; 37-48; 55-69; 73-83; 91-119; 123-126; 135-142; 152-163; 169-199; 204-222; 229-238; 248-266; 284-289; 300-308; 321-335; 343-350; 384-398; 407-412; and 419-430; wherein (a) the variant has phytase activity; and (b) each position corresponds to a position of SEQ ID NO, 2; and (c) the variant has a percentage of identity to SEQ ID NO: 2 of at least 75%, and (d) with the proviso that the variant is not a variant of the Peniophora lycii CBS 686.96 phytase selected from the group consisting of: G26S; Y28N; D29S; F31Y; E42D,K,A; T45R; V46I; W59F; S62D; A64K; R65Q,E,G,A; S66T; R67A,I,Q,K; Q68Y,V,I; K74R,A; A99N; D100S L102V; P103E; F104L; N107T,Q; S109M; H110S,V Q111E,N; T112A,S; M116F,I,L; A135S; D137Y; Q138D,S,G; D142A; ( )154fH; S155N; G156P; E157Q,S; E169S; E170A,P; G171A; ( )171aS; T174P,A; N177S; N178S,T; M179T,V,L; N182A,V; V184G; D185E; ( )185eT; G186S,A; D187R,K,S, ( )187aV,T; E188Q,S,A,V; S189E; V195L; N199A,P; L204N; A207D,H; S216T; D217E; L219Y,T; T220Y,N; L221F; M222L; L230V; ( )235eE,Q; V248R,I; S249Q,A; E251D; Y254A,L,G; D255N; D257K; Y259F; T262H; P264A; G286R,H; Q287K,S; A288P; T321Q,S,G; M322I; V323I; P324A; A327S,F,I,L; F332Y; N333P; E344R; R346P, W348F; V349L,A,S; D350S,V; G388A; V393R; E395K,T; and L396R. The present invention also relates to isolated nucleic acid sequences encoding the phytase variant and to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences as well as methods for producing and using the phytase variants. Continue reading about Phytase variants... Full patent description for Phytase variants Brief Patent Description - Full Patent Description - Patent Application Claims Click on the above for other options relating to this Phytase variants patent application. ###  How KEYWORD MONITOR works... a FREE service from FreshPatents1. Sign up (takes 30 seconds). 2. Fill in the keywords to be monitored. 3. Each week you receive an email with patent applications related to your keywords. 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